LOCUS       DAZKMZ010000001       130086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000001 DAZKMZ010000000
VERSION     DAZKMZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 130086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.001"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8M5_000001"
     CDS             212..604
                     /locus_tag="ACG8M5_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0357293.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8M5_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8M5_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0357294.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8M5_000003"
     CDS             2698..2943
                     /locus_tag="ACG8M5_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357295.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8M5_000004"
     CDS             3165..4775
                     /locus_tag="ACG8M5_000004"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0357296.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8M5_000005"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8M5_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357297.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8M5_000006"
     CDS             5067..5936
                     /locus_tag="ACG8M5_000006"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0357298.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8M5_000007"
     CDS             6101..7372
                     /locus_tag="ACG8M5_000007"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0357299.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8M5_000008"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8M5_000008"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0357300.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8M5_000009"
     CDS             8763..9026
                     /locus_tag="ACG8M5_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0357301.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8M5_000010"
     CDS             9471..9920
                     /locus_tag="ACG8M5_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0357302.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8M5_000011"
     CDS             9977..11395
                     /locus_tag="ACG8M5_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0357303.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8M5_000012"
     CDS             11395..12255
                     /locus_tag="ACG8M5_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0357304.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8M5_000013"
     CDS             12429..13202
                     /locus_tag="ACG8M5_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0357305.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8M5_000014"
     CDS             13349..13714
                     /locus_tag="ACG8M5_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0357306.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8M5_000015"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8M5_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0357307.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8M5_000016"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8M5_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357308.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8M5_000017"
     CDS             15560..16216
                     /locus_tag="ACG8M5_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0357309.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8M5_000018"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8M5_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0357310.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8M5_000019"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8M5_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0357311.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8M5_000020"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8M5_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0357312.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8M5_000021"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8M5_000021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0357313.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8M5_000022"
     CDS             21888..22202
                     /locus_tag="ACG8M5_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0357314.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8M5_000023"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8M5_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0357315.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8M5_000024"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8M5_000024"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0357316.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8M5_000025"
     CDS             23546..23875
                     /locus_tag="ACG8M5_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0357317.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8M5_000026"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8M5_000026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0357318.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8M5_000027"
     CDS             24863..25687
                     /locus_tag="ACG8M5_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0357319.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8M5_000028"
     CDS             25680..26027
                     /locus_tag="ACG8M5_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0357320.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8M5_000029"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8M5_000029"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0357321.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8M5_000030"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8M5_000030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0357322.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8M5_000031"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8M5_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0357323.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8M5_000032"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8M5_000032"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0357324.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8M5_000033"
     CDS             31684..32451
                     /locus_tag="ACG8M5_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357325.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8M5_000034"
     CDS             32441..34483
                     /locus_tag="ACG8M5_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357326.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8M5_000035"
     CDS             34764..34913
                     /locus_tag="ACG8M5_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0357327.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8M5_000036"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8M5_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0357328.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8M5_000037"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8M5_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0357329.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8M5_000038"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8M5_000038"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0357330.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8M5_000039"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8M5_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0357331.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8M5_000040"
     CDS             39330..40412
                     /locus_tag="ACG8M5_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0357332.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8M5_000041"
     CDS             40412..41215
                     /locus_tag="ACG8M5_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0357333.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8M5_000042"
     CDS             41285..42025
                     /locus_tag="ACG8M5_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0357334.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8M5_000043"
     CDS             42036..43040
                     /locus_tag="ACG8M5_000043"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0357335.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACG8M5_000044"
     CDS             complement(43085..44929)
                     /locus_tag="ACG8M5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357336.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="ACG8M5_000045"
     CDS             45140..45658
                     /locus_tag="ACG8M5_000045"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0357337.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="ACG8M5_000046"
     CDS             45786..46469
                     /locus_tag="ACG8M5_000046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0357338.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="ACG8M5_000047"
     tRNA            46778..46851
                     /locus_tag="ACG8M5_000047"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="ACG8M5_000048"
     CDS             47010..47369
                     /locus_tag="ACG8M5_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0357339.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="ACG8M5_000049"
     CDS             47492..49123
                     /locus_tag="ACG8M5_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0357340.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="ACG8M5_000050"
     CDS             complement(49185..49826)
                     /locus_tag="ACG8M5_000050"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0357341.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="ACG8M5_000051"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="ACG8M5_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0357342.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="ACG8M5_000052"
     CDS             50443..50787
                     /locus_tag="ACG8M5_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0357343.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="ACG8M5_000053"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="ACG8M5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0357344.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="ACG8M5_000054"
     CDS             51527..54334
                     /locus_tag="ACG8M5_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0357345.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="ACG8M5_000055"
     CDS             54525..55016
                     /locus_tag="ACG8M5_000055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0357346.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /locus_tag="ACG8M5_000056"
     CDS             55119..56108
                     /locus_tag="ACG8M5_000056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0357347.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="ACG8M5_000057"
     CDS             56141..56944
                     /locus_tag="ACG8M5_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0357348.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="ACG8M5_000058"
     CDS             56965..57879
                     /locus_tag="ACG8M5_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0357349.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="ACG8M5_000059"
     CDS             57962..58336
                     /locus_tag="ACG8M5_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0357350.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="ACG8M5_000060"
     CDS             58475..58720
                     /locus_tag="ACG8M5_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0357351.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="ACG8M5_000061"
     CDS             58892..59956
                     /locus_tag="ACG8M5_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0357352.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="ACG8M5_000062"
     CDS             60202..60627
                     /locus_tag="ACG8M5_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0357353.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="ACG8M5_000063"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="ACG8M5_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0357354.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="ACG8M5_000064"
     CDS             61166..62020
                     /locus_tag="ACG8M5_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0357355.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /gene="xseA"
                     /locus_tag="ACG8M5_000065"
     CDS             62021..63358
                     /gene="xseA"
                     /locus_tag="ACG8M5_000065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0357356.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="ACG8M5_000066"
     CDS             63363..63587
                     /locus_tag="ACG8M5_000066"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0357357.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="ACG8M5_000067"
     CDS             63589..64479
                     /locus_tag="ACG8M5_000067"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0357358.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="ACG8M5_000068"
     CDS             64488..65306
                     /locus_tag="ACG8M5_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0357359.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="ACG8M5_000069"
     CDS             65413..65862
                     /locus_tag="ACG8M5_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0357360.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="ACG8M5_000070"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="ACG8M5_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0357361.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="ACG8M5_000071"
     CDS             complement(67617..67733)
                     /locus_tag="ACG8M5_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0357362.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="ACG8M5_000072"
     CDS             complement(67806..68762)
                     /locus_tag="ACG8M5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0357363.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="ACG8M5_000073"
     CDS             68933..69301
                     /locus_tag="ACG8M5_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0357364.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="ACG8M5_000074"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="ACG8M5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0357365.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="ACG8M5_000075"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="ACG8M5_000075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0357366.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="ACG8M5_000076"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="ACG8M5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0357367.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="ACG8M5_000077"
     CDS             71289..73481
                     /locus_tag="ACG8M5_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0357368.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /gene="mraY"
                     /locus_tag="ACG8M5_000078"
     CDS             73507..74469
                     /gene="mraY"
                     /locus_tag="ACG8M5_000078"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0357369.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /gene="murD"
                     /locus_tag="ACG8M5_000079"
     CDS             74483..75844
                     /gene="murD"
                     /locus_tag="ACG8M5_000079"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0357370.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="ACG8M5_000080"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="ACG8M5_000080"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0357371.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="ACG8M5_000081"
     CDS             76973..78193
                     /locus_tag="ACG8M5_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0357372.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="ACG8M5_000082"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="ACG8M5_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0357373.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="ACG8M5_000083"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="ACG8M5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0357374.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="ACG8M5_000084"
     CDS             80955..81632
                     /locus_tag="ACG8M5_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0357375.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="ACG8M5_000085"
     CDS             81646..82245
                     /locus_tag="ACG8M5_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0357376.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82326..82547
                     /locus_tag="ACG8M5_000086"
     CDS             82326..82547
                     /locus_tag="ACG8M5_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0357377.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="ACG8M5_000087"
     CDS             82596..83378
                     /locus_tag="ACG8M5_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0357378.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="ACG8M5_000088"
     CDS             83399..84106
                     /locus_tag="ACG8M5_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0357379.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="ACG8M5_000089"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="ACG8M5_000089"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0357380.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /gene="zwf"
                     /locus_tag="ACG8M5_000090"
     CDS             complement(87215..88741)
                     /gene="zwf"
                     /locus_tag="ACG8M5_000090"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0357381.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="ACG8M5_000091"
     CDS             complement(88756..89403)
                     /locus_tag="ACG8M5_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0357382.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="ACG8M5_000092"
     CDS             complement(89587..89916)
                     /locus_tag="ACG8M5_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0357383.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="ACG8M5_000093"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="ACG8M5_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0357384.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="ACG8M5_000094"
     CDS             90837..91850
                     /locus_tag="ACG8M5_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0357385.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="ACG8M5_000095"
     CDS             91850..93127
                     /locus_tag="ACG8M5_000095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0357386.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="ACG8M5_000096"
     CDS             93190..95892
                     /locus_tag="ACG8M5_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0357387.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="ACG8M5_000097"
     CDS             96044..96361
                     /locus_tag="ACG8M5_000097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0357388.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="ACG8M5_000098"
     CDS             96391..96711
                     /locus_tag="ACG8M5_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0357389.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="ACG8M5_000099"
     CDS             96819..98279
                     /locus_tag="ACG8M5_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0357390.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="ACG8M5_000100"
     CDS             98347..98568
                     /locus_tag="ACG8M5_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357391.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="ACG8M5_000101"
     CDS             98599..98781
                     /locus_tag="ACG8M5_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0357392.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="ACG8M5_000102"
     CDS             98781..99194
                     /locus_tag="ACG8M5_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0357393.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="ACG8M5_000103"
     CDS             99317..100498
                     /locus_tag="ACG8M5_000103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357394.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="ACG8M5_000104"
     CDS             complement(100569..100733)
                     /locus_tag="ACG8M5_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357395.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="ACG8M5_000105"
     tRNA            100887..100960
                     /locus_tag="ACG8M5_000105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(101029..102168)
                     /locus_tag="ACG8M5_000106"
     CDS             complement(101029..102168)
                     /locus_tag="ACG8M5_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0357396.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            102340..102474
                     /locus_tag="ACG8M5_000107"
     CDS             102340..102474
                     /locus_tag="ACG8M5_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357397.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(102467..103102)
                     /locus_tag="ACG8M5_000108"
     CDS             complement(102467..103102)
                     /locus_tag="ACG8M5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357398.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103215..103850)
                     /locus_tag="ACG8M5_000109"
     CDS             complement(103215..103850)
                     /locus_tag="ACG8M5_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0357399.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103884..104345)
                     /locus_tag="ACG8M5_000110"
     CDS             complement(103884..104345)
                     /locus_tag="ACG8M5_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0357400.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104475..104906)
                     /locus_tag="ACG8M5_000111"
     CDS             complement(104475..104906)
                     /locus_tag="ACG8M5_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0357401.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104924..105244)
                     /locus_tag="ACG8M5_000112"
     CDS             complement(104924..105244)
                     /locus_tag="ACG8M5_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0357402.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105543..106319
                     /locus_tag="ACG8M5_000113"
     CDS             105543..106319
                     /locus_tag="ACG8M5_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0357403.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106334..106537
                     /locus_tag="ACG8M5_000114"
     CDS             106334..106537
                     /locus_tag="ACG8M5_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357404.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106553..106891
                     /locus_tag="ACG8M5_000115"
     CDS             106553..106891
                     /locus_tag="ACG8M5_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0357405.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106878..107057
                     /locus_tag="ACG8M5_000116"
     CDS             106878..107057
                     /locus_tag="ACG8M5_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357406.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107100..107570)
                     /locus_tag="ACG8M5_000117"
     CDS             complement(107100..107570)
                     /locus_tag="ACG8M5_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357407.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107657..108355
                     /locus_tag="ACG8M5_000118"
     CDS             107657..108355
                     /locus_tag="ACG8M5_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0357408.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108533..108874
                     /locus_tag="ACG8M5_000119"
     CDS             108533..108874
                     /locus_tag="ACG8M5_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357409.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108867..109538
                     /locus_tag="ACG8M5_000120"
     CDS             108867..109538
                     /locus_tag="ACG8M5_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0357410.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109544..110236
                     /locus_tag="ACG8M5_000121"
     CDS             109544..110236
                     /locus_tag="ACG8M5_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0357411.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            110233..110982
                     /locus_tag="ACG8M5_000122"
     CDS             110233..110982
                     /locus_tag="ACG8M5_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357412.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110994..111263
                     /locus_tag="ACG8M5_000123"
     CDS             110994..111263
                     /locus_tag="ACG8M5_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357413.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111269..111433
                     /locus_tag="ACG8M5_000124"
     CDS             111269..111433
                     /locus_tag="ACG8M5_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357414.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111426..111725
                     /locus_tag="ACG8M5_000125"
     CDS             111426..111725
                     /locus_tag="ACG8M5_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357415.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111725..112027
                     /locus_tag="ACG8M5_000126"
     CDS             111725..112027
                     /locus_tag="ACG8M5_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0357416.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112067..112432
                     /locus_tag="ACG8M5_000127"
     CDS             112067..112432
                     /locus_tag="ACG8M5_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357417.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112922..113062
                     /locus_tag="ACG8M5_000128"
     CDS             112922..113062
                     /locus_tag="ACG8M5_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357418.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113059..113241
                     /locus_tag="ACG8M5_000129"
     CDS             113059..113241
                     /locus_tag="ACG8M5_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357419.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            113254..113448
                     /locus_tag="ACG8M5_000130"
     CDS             113254..113448
                     /locus_tag="ACG8M5_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357420.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113481..113693)
                     /locus_tag="ACG8M5_000131"
     CDS             complement(113481..113693)
                     /locus_tag="ACG8M5_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357421.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            113821..114021
                     /locus_tag="ACG8M5_000132"
     CDS             113821..114021
                     /locus_tag="ACG8M5_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357422.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            114018..114290
                     /locus_tag="ACG8M5_000133"
     CDS             114018..114290
                     /locus_tag="ACG8M5_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0357423.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114291..114476
                     /locus_tag="ACG8M5_000134"
     CDS             114291..114476
                     /locus_tag="ACG8M5_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357424.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114454..114810
                     /locus_tag="ACG8M5_000135"
     CDS             114454..114810
                     /locus_tag="ACG8M5_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357425.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            114770..115246
                     /locus_tag="ACG8M5_000136"
     CDS             114770..115246
                     /locus_tag="ACG8M5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0357426.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115392..116120)
                     /locus_tag="ACG8M5_000137"
     CDS             complement(115392..116120)
                     /locus_tag="ACG8M5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0357427.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            116168..116446
                     /locus_tag="ACG8M5_000138"
     CDS             116168..116446
                     /locus_tag="ACG8M5_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357428.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            116448..116834
                     /locus_tag="ACG8M5_000139"
     CDS             116448..116834
                     /locus_tag="ACG8M5_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0357429.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            116831..117211
                     /locus_tag="ACG8M5_000140"
     CDS             116831..117211
                     /locus_tag="ACG8M5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0357430.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            117323..117775
                     /locus_tag="ACG8M5_000141"
     CDS             117323..117775
                     /locus_tag="ACG8M5_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0357431.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            117772..119499
                     /locus_tag="ACG8M5_000142"
     CDS             117772..119499
                     /locus_tag="ACG8M5_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0357432.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            119563..120750
                     /locus_tag="ACG8M5_000143"
     CDS             119563..120750
                     /locus_tag="ACG8M5_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0357433.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            120722..121291
                     /locus_tag="ACG8M5_000144"
     CDS             120722..121291
                     /locus_tag="ACG8M5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0357434.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            121304..122668
                     /locus_tag="ACG8M5_000145"
     CDS             121304..122668
                     /locus_tag="ACG8M5_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0357435.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            122670..122951
                     /locus_tag="ACG8M5_000146"
     CDS             122670..122951
                     /locus_tag="ACG8M5_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0357436.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            122929..123255
                     /locus_tag="ACG8M5_000147"
     CDS             122929..123255
                     /locus_tag="ACG8M5_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0357437.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            123245..123583
                     /locus_tag="ACG8M5_000148"
     CDS             123245..123583
                     /locus_tag="ACG8M5_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357438.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            123573..123938
                     /locus_tag="ACG8M5_000149"
     CDS             123573..123938
                     /locus_tag="ACG8M5_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0357439.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            123945..124571
                     /locus_tag="ACG8M5_000150"
     CDS             123945..124571
                     /locus_tag="ACG8M5_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0357440.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            124571..125035
                     /locus_tag="ACG8M5_000151"
     CDS             124571..125035
                     /locus_tag="ACG8M5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357441.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            125230..127539
                     /locus_tag="ACG8M5_000152"
     CDS             125230..127539
                     /locus_tag="ACG8M5_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0357442.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            127551..128255
                     /locus_tag="ACG8M5_000153"
     CDS             127551..128255
                     /locus_tag="ACG8M5_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0357443.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            128252..>130086
                     /locus_tag="ACG8M5_000154"
     CDS             128252..>130086
                     /locus_tag="ACG8M5_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0357444.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        43160 a        21840 c        27231 g        37855 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgatcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
   100981 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
   101041 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
   101101 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
   101161 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
   101221 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
   101281 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
   101341 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
   101401 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
   101461 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
   101521 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
   101581 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
   101641 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
   101701 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
   101761 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
   101821 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
   101881 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
   101941 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
   102001 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
   102061 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
   102121 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
   102181 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
   102241 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
   102301 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
   102361 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
   102421 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
   102481 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
   102541 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
   102601 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
   102661 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
   102721 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
   102781 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
   102841 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
   102901 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
   102961 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
   103021 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
   103081 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
   103141 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
   103201 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
   103261 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
   103321 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
   103381 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
   103441 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
   103501 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
   103561 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
   103621 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
   103681 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
   103741 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
   103801 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
   103861 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
   103921 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
   103981 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
   104041 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
   104101 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
   104161 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
   104221 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
   104281 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
   104341 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
   104401 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
   104461 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
   104521 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
   104581 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
   104641 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
   104701 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
   104761 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
   104821 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
   104881 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
   104941 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
   105001 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
   105061 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
   105121 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
   105181 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
   105241 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
   105301 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
   105361 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
   105421 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
   105481 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
   105541 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
   105601 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
   105661 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
   105721 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
   105781 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
   105841 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
   105901 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
   105961 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
   106021 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
   106081 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
   106141 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
   106201 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
   106261 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
   106321 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
   106381 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
   106441 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
   106501 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
   106561 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
   106621 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
   106681 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
   106741 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
   106801 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
   106861 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
   106921 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
   106981 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
   107041 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
   107101 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
   107161 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
   107221 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
   107281 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
   107341 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
   107401 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
   107461 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
   107521 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
   107581 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
   107641 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
   107701 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
   107761 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
   107821 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
   107881 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
   107941 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
   108001 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
   108061 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
   108121 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
   108181 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
   108241 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
   108301 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
   108361 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
   108421 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
   108481 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
   108541 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
   108601 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
   108661 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
   108721 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
   108781 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
   108841 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
   108901 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
   108961 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
   109021 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
   109081 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
   109141 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
   109201 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
   109261 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
   109321 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
   109381 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
   109441 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
   109501 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
   109561 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
   109621 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
   109681 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
   109741 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
   109801 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
   109861 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
   109921 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
   109981 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
   110041 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
   110101 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
   110161 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
   110221 tggaggggaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
   110281 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
   110341 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
   110401 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
   110461 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
   110521 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
   110581 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
   110641 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
   110701 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
   110761 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
   110821 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
   110881 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
   110941 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
   111001 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
   111061 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
   111121 gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga acgcaattaa
   111181 gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg caaaaacatc
   111241 ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga
   111301 attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt ggctcaacac
   111361 atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
   111421 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
   111481 ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
   111541 aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag
   111601 taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat attatttggg
   111661 atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat gtggaggaga
   111721 aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg gattggatca
   111781 agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag ttgccgcttc
   111841 tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag tagtaacctt
   111901 gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt ttgcttatga
   111961 cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt ccagtgacct
   112021 gaaataattc cattaatgga agaaataact gaataggagg atagacatga ttgatgcaaa
   112081 acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc taccatttct
   112141 taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt atgttgtaat
   112201 tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta ttaactgttg
   112261 ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg aatattgtcg
   112321 tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc taaacatcaa
   112381 cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct aaccaccaat
   112441 atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt aaggttcttt
   112501 acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat ccaaagctct
   112561 cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag aacctgagag
   112621 ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt gtaaacacat
   112681 aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt cagtaggaaa
   112741 aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc tgcaattaaa
   112801 ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag aaagtcaagc
   112861 agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg aaggcgatag
   112921 tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg atcaaatcga
   112981 attagctaga caatactttg ttccatatgc agatacctgg tccaagcata agaatgacat
   113041 catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga ggaagccaaa
   113101 tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca aggagttaag
   113161 ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt gcttttgctc
   113221 catctagcag aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa
   113281 acagggaaaa tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa
   113341 acttacacga acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat
   113401 cagattatgc cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc
   113461 agccgtgtgg gctggagttg ctatttcttt ttaataatga gtttttctaa atttaattca
   113521 ttctcacgag tttgcgtcca ataatcagaa taatatttta attgtttaag agtaggaatg
   113581 tcgtcttcgg taaaattaga aaataattct ggagaattat ctttttttaa ttgttctaat
   113641 cctgattctt taaaacgttg ttcaacgagt tttactaaat tgttgatatt catgataaat
   113701 caccttttta aatttaattt cgatttgatt aatcgataca agtattatat caaataaaaa
   113761 agctgattta gggggtctta caaaaacact aaaatttttt caaaaagagg aggaacggtg
   113821 gtgaaagtca gagaacaatt caagatagtg agcgacgaag gtaatgttta caaaataaag
   113881 atcgttaata ttaatgaata tcgagaagca gatcacaaat atttggttga cttatacatg
   113941 aacgataaat atttgggaga aggattttgt ggagattcac tttttgatga tgagcgaatt
   114001 ttgaaggagg aaacagaatg atccacgaac taaaaatact accagaatat tttgaagcag
   114061 tgacgagcgg acgcaaacaa ttcgagatcc gcaagaatga tcgtgacttc aaagttggtg
   114121 atcagttaat actacgtgaa tataaaaaat acattaaaca tgaattacct ggactaacga
   114181 gaccaacatt tagttacact ggtgactcat acaaagcaga gatcacttat attactgatt
   114241 acatgcagaa gtccggatat gtagtgttgg ggattaaagg ggtgaattaa atgaaagtat
   114301 acatcttagc tatcacagag ggcacatgga tgttcccagt cggttccgga aaaatctata
   114361 aatcaaaaac agctgcttat aaagcatttg aaaaatacaa aaaagaaaac ggtggcggca
   114421 ctaacgcaaa gattctagtg gctgataact ggcatgaaga aggtgaacgt aattgattta
   114481 cctgttcatt ttcgtggtac taactatttt tatgctagta atcttattca aaaaaagatg
   114541 agtaaagacg aacgaaaaat tctggccaac ttttatttag tattaaaaat cgaacaaaat
   114601 gtgttttcgg aaaggagaat tgtatgggaa aaacaaaatc aaagattaag aagaaaaaac
   114661 gacgtttgga acaaaaggca atccagaatg gaacggctaa gaaaaaataa aaaagctacc
   114721 cctttcgaga tagcagcgca atactattct accataaggg gtggcgtttg tgagatttca
   114781 ttggttaaaa gattaccaag aactagatga gcagattctt tacttaaagt ggaatcttaa
   114841 taaaagtaag cttgaattga atcgatgggt ctgtggtgat ttagcaaacg tccgcatcga
   114901 aaagaattcg agaacttctt ctttagaaga gaatatccaa aagatagaaa atgaattgga
   114961 attgttgatc gagcaaaaag aagaaatgtt attgctgata gatagctttt ctggtatcga
   115021 taatcaaatt gttaagatga aatatgttga tcaaatgagc ttagaagata ttgctgaatc
   115081 tgttgggtat agctcatcgt atgtcagaca gcgacatgca gaaattagaa agacattgaa
   115141 ctttttagat gagtatgaac acagacaagc tgaccgattg aaaaaagaaa acgaaattga
   115201 tttttacaat agcgagaagt acaaagaaca actctcttta ttctaaaatt acactgttca
   115261 ctaaatgttc ggtcattgta gctatgtaaa cattgttagc agcatgatat tctattagtg
   115321 tcaaaaaaat atgaaagagc caagatatcc caaccgtttt attaattggt atctgtggct
   115381 cttttattct tttaattagt agttggcata aagttatttc catttataaa ttgatcagct
   115441 aaatcagcat cataatcagc cgcgataaat tgcgctgctg atttaataaa tgcatccata
   115501 tcatgaacat ctttattagt atgtctacga acatagtgcg tttcatcatt accaatccaa
   115561 gtagcagctt tggctaatct ttggagttta ggaaaatcat tcaaataatt ttcaattgtt
   115621 ttacctaata acattttttt tattttatct tcatcctctg aatttttaga tattgcataa
   115681 tctttaatta gaaattctag agcttttcta taaccaactc cagcaatttg ttccaattgc
   115741 tcagatttag cttgtgtggc ttgagtataa atatcaacaa atgtaggaga aactttttca
   115801 atattttctg gtaaatctat tttaatcggc ggacggtaat aatctttaat taaaatattt
   115861 tcagaatatc cttcatcttc attaaacgta tttataaatt gtaaactata aaatttgttg
   115921 cactcatcat ttatacactg tactaatacg ccaaagattc tatcattatc gaacgcacta
   115981 tatgatgaaa ccccaaaata cacatacgga gtcattatat ttccgcaatg cggacaacta
   116041 tttggcaaat aaattgtctc tgtattttga cgatcacgat acttaatatt aacttcttga
   116101 agcattaaaa cacctcgcat cttttttata aatcatacca caaaatatct gataggagag
   116161 taaacaaatg aatgattttc atgaagcggt acttagtatt gaagtagaat caagtttggc
   116221 taaagtttat aagaaagcaa ttgaagcaga gaatagtccg tacagagaaa attggaatgg
   116281 taatcatgca catgtgcaag tggagaatga tgattacaga actggaatga atacgttagt
   116341 tatttcttta ttgtcacaca ctctacccaa tctgcaggaa acgattgaat ggtatgagcg
   116401 aatgggcgca aaagtaatcc gtacaaatta caaaggagag aattaaaatg gcaatgatca
   116461 aaattagaac atccattagt ggaacagagt attgggattc agaaaagaaa aggacagtag
   116521 ttgttccgaa agatcaagaa ccaaattttg aagtaaaaga agaaaaagga attttgagtg
   116581 atggcaaatc atttgttgct gtcaatggcg aactcatagc tcatagcagt gaaattcttg
   116641 atagcgatgg aaacactgct gctaattttg atggagacga agctactaat gatcagtcca
   116701 ctgaagatac ggatgaactg gacaatatga ctgcaaaaga attgcgtgca tatgctaaga
   116761 aacatggtat tgatattcct ggtgctatcc gtgcaaaagg cgatatcata aatcttattc
   116821 gtgaagtaga atgaaatact gtcagtttga cggatgcacg aacaaaatag caaagggtat
   116881 ctactgtact gaacacaaga gatcaagcag atcacgcaag aagaagcagc aagcaaagtc
   116941 tgtttatcat catgagaaca aaccattcta tcgaacgcaa gcatggaaag atatgcgtca
   117001 atttatttat gaaagagaag gtggtcattg tcagcggtgt ggtcagttca tatttggaaa
   117061 gagagcacac gtccatcaca ttgtaccaat caaagacaac gaactgctta agcttgatcc
   117121 aaacaatctc atgctattat gttcaaaatg tcatccaatt gttgaaaacg aaacggaaga
   117181 caaaaaagtt tttccttcgt atttcaattg aagcccccct attcattttc aaaatttttt
   117241 cgcgtgggga gatagggtag cggggagtca cgcgcatcgt taggtcaaat ttttcaaaaa
   117301 acaaaggggg gtgtatacaa aaatgacgac taaagcgcaa cgtaaagcga ttattgatga
   117361 aaaagtaagt gctgaaaaag ctcgtatctt agaaataatg aatttgtctg atttgtacac
   117421 catcactctt gatccattaa tcgaatcata cttggatatt tttgaaattt accaacacaa
   117481 atatttattg tggaaggaaa aaggatttcc ggagacgcaa aaattcacga ataaatcagg
   117541 agctactaat caatcaaaac atccattggc acaacaagta gaaacttggg cagataagaa
   117601 gatgaaagct ctagatttat taggattaac aaacaaagca aaaactggta gactaattgc
   117661 tggaggatcg accgcaagaa aagatgaaga aattacacgc ccagaagtaa agccagtaga
   117721 tgaactagca gcgcacagaa ataaatggcg taagaaggca ggtgctgaaa aatgattgaa
   117781 cctggtgtaa attatgctga tttatttgca aaagaagtaa gaaagaaacc taagaagtat
   117841 cctaagaccg ttcgtttagc aatagatcgc tggtatcagt ggaaaaaaag aaaagatatt
   117901 tggtttgatg tagatcgcgc gaatgaaatg atggattggg tagaatcatt tattgttcac
   117961 acaaaaggag aaatggtggg aaaaccgttt cttttagaac cctgggaaaa atttatttat
   118021 tcttggattt atggttgggt taaagaaaat gaaaaaggcc aaattgtacg cgttactcgt
   118081 gaggcttatg tccagattcc aaaaaagaat ggtaaaacat tgattgctgt cggttcatta
   118141 ggatatgcaa tgtacggaga aggggcgtta tcagttgatt gctatgcttg cgcatctgat
   118201 tttgctcaag cgcaatatgc tgccaaacct tttgcagtta caatcttaaa caatccaatc
   118261 ctacttgaag gaactaaaat atttaggggg ccaaagggaa ctgtttctag tattacttat
   118321 gactatattc atgaagatat ggcttactca aataaattta ttgttcaaac caagaatatt
   118381 gataatatcg agggatccaa tccatatttt gtattgaacg atgaattgca taaacaggag
   118441 aaaatggaac agtatgacaa ttttaagtct gctcagatat ctttaccaca gccattgatg
   118501 tttaatatct caactgctgg gaaaggttcg tcgtctgttg gaatgcgtgt atatcgcgaa
   118561 gcaaaagaag tgttgaaacg tgatgataat gattcaaact ttgttctaat ctatgagcca
   118621 aataaaaatt atgattggac ggatagaaaa gtctgggaaa tgtgcaatcc taactgggga
   118681 atatcagtcg atttatctgc tttagaatcg gcatttaaaa cagcgcaacg gtcagctcat
   118741 tccaaagctg aatttctaac gaaacacttg gatgtatttg tgaacggcgc ggataatttc
   118801 tttgaacagg atcaagtaga gccatgttta gttaccacac aagaacttgg tgatttaagt
   118861 ggcgaaccat gttatatcgg tttagattta tcacgtacac gagatttgac ctgtgtatct
   118921 ttaaacttcc caacatggga tgaagatggt aaagcggttc ttaaagtaaa gcaactttat
   118981 tttattccga atgaagattt agagtttcga gaaaaagaag ataatgttcc ttacagtgat
   119041 ttggctgaac aaggtttcgt tgaattttgc gacggaaaaa tgattgatca agatcagatt
   119101 ttgcagtaca tagaggactg catgaattta tatgatattc agcaagtgaa ttatgatcca
   119161 gcgatgagtg acaaacttgt tgagaaatta gaaaacttag gattggaatg cgttgaagtt
   119221 gctcaatatc ctaaagtatt gaatgctcct tttgacgatg tcgaacggtt gttttatgag
   119281 aaacgaattc aatttgataa tccattattc ctttattgca ctttgaacgt tgtagcaatt
   119341 actaacatca atggacaaaa agcgccaagt aaacgtcaat caaagaaaaa gattgatggg
   119401 ttcgtggcgt ttttgtgtgg tcataaggaa acgatgaatc aaatgacaga tattgattca
   119461 gatgagttag atgattacct aagttccatc tatagataaa tagaaaggcg gtgaaaaaaa
   119521 ttgaaattac gtgatcggtt atcgaatgca gtctattctt ttatggaaaa acgtggatat
   119581 atcgaggata tttttggaca ctacacacgt tatggtcaaa gatatgtgac ggattcatct
   119641 atcatggaat cttctgatgt ttatgaatta gttcaagaca tatctaatca ggtggcattg
   119701 gccacaccaa ttgttattgg tcccgacggc aacgaagtca aagatcatca cttactaaat
   119761 attttgaaga gtcccaatga ttatttgaca ggattcgaat ttaccaaatt ggaaacaaat
   119821 actttattga tcaatggtga gacattccca ttaacggata gggatcagct tcatttggcg
   119881 tacggtgtaa caactaaaat caatgaacga cttcaagaag aatttgaaat gaatggtcaa
   119941 aaaatacctg gtcaaatgat tcgacacatt aagaacatcg gaactgattc attaaaaggt
   120001 gctggaataa ttgatcttgc aagaaacact ctagaaggcg ttctgagcgc tgaaaaagtt
   120061 ttgacggaca aatatactaa aggcggttta cttgcgttca tgcttaaact agacgcccac
   120121 atcaatccaa ataatagcgc ccaaacaaaa attgtcaaag ctatattgga tcaactggaa
   120181 ggaacgcaaa atgagagtga tcattctgtt aagatgattc ctttgggaaa aggatattcc
   120241 atcgagacat taaaaagtcc tgttgatgac gcggcaattt tgaattattt aggtgtttac
   120301 aaaaaagact taggaaaatt tctaggaatc aatgttgata cgtatcaatc gctgatgaag
   120361 acagatattg aaaaagcgat gatgtatctg cacaacaaag caatcaaacc aatattgaaa
   120421 aacaagagcg aacattacac cgctcttttt tttatgccta attctggcta tagagtggaa
   120481 tggaaaatta atattttgga ttttgttcct tactcaacaa aaacaaatat tggctacaac
   120541 atcgttcgta ccgggattac aagtccagat aacgtggcag aaatgcttgg ttttcctaaa
   120601 cagaatactc cagaaacaca agctatctat atttcaaatg acttatctag gattggccag
   120661 aaaaatgcaa cagatgattc cttaccaacg aatgatcaag acttgaaagg aggtgatgga
   120721 aatgaagaag aaggaaattc gcacgattga catcaccaat ctttcaacac gttccgatga
   120781 agaaactcat acgaggacca ttagcggcta cgctgctgta ttcaacagtc aaacactttt
   120841 atgggatgac ttaagcgaag tgattgttcc tggtgcattc tcaaaggcaa ttagtaattc
   120901 ggatgttcgt tgtttgttca atcacgattg gtccaatgtg ctaggacgta ctaaaagcgg
   120961 aacccttcgc cttgaagaag atgaacgtgg tttgaaattc gaagttgatt taccggacac
   121021 aacggtagca agggacttag ttaaatctat ggaacgagga gacatcaacc aatgtagttt
   121081 tggttttgtg ccgactgaag aaacgtggga ctacaattct gaaccaatgc ttcgaacaat
   121141 ccatgaagtg gaattatacg aggtttctat tgtgcctttg ccagcatacg aagatacaga
   121201 agctgcatta agaagtcgtg atgaattaga aaaaaacgtc gaagaaagaa aaaaattaat
   121261 caaaaaaatt aatcaagcgc tagaagcgta ggaggaaaaa tacatggata aagaattatt
   121321 gaaaaaaatg aaggcacgtc gtgagcaacg attgactgaa ttacgtgaaa aagttgaatc
   121381 aggagaatta cgcgaagcag atttagaagc cgtgaaagaa gaaattgaca gtgttattga
   121441 tgaattgaac ggaattaaag acgaattagg cgcagattct ggaactgatg aaacagacga
   121501 caatacagat gatcaatcga atagtacgga ttcagacgaa agccgttctg gtgaagacaa
   121561 cgatcaagaa gaagattcag acagtgaaga tagttcagaa aatcgttctg gaatgattac
   121621 tcaacagcaa cgagatggat tacttggatc aattaagaac ggattggagg cacgtgcaaa
   121681 aatgaccaag aaacaaaaag atcaacaact acgaaaagca tttgctaatt ttgtagctgg
   121741 aaatatttct gaagcagaag ctcgagcttt agggattgaa gctggcaacg gttcagttac
   121801 tgtcccagaa gtaattgcat ctgaagttat tacttatgct caagaagaaa atttacttcg
   121861 taaatacgga acagtggtgc gaacatcagg agatgtcaaa tatccaattc ttgtgaagaa
   121921 agcagatgcg aatgtaaaca agaaagagcg ttcaactgat attgctgaaa cagctattca
   121981 gtttgatgaa attttgctta atcctgccga attcgatgct ttggcaacag taactaaaaa
   122041 attattaaaa atgtctggtg ttccagttga agatattgtt gtggaagaat tgaaaaaagc
   122101 ttatgtgcgt aaagaaatca attatatgtt caatggtgat gacgctggaa atgaaaatcc
   122161 tggtgcatta gccaaaaagg ctgtagcatt taaaaaacct ttagatctaa ctgcttcagg
   122221 tgctgggcaa aaattatatg atgcattaat cgaatttaaa aatacaccag tgacagaagt
   122281 gatgaaaaag ggacgcttta ttattaatcg agctgctttg actgccattg aaaaaatgaa
   122341 aacagatgat ggatttcctt tgttgcggcc attcacacaa gcagaaggtg gaattggtta
   122401 ccaattagtt ggctatcctg tggattggac agatgcagca gataaaaagg gtgaaccaga
   122461 cacgccagtt ttatattttg gcgatttttc tgcatttaaa attcaagaag ttattggtgc
   122521 cttggaaatt caaaaacttg ttgaaaaatt ctctggtaaa aatcaaattg gattccaaat
   122581 ttacaacttg ctagatggtc aattggttta ttctccattt gaaccggcag tatatcgcta
   122641 cgaaattaca aaaccagttg gtggttaaga tggaagagca aactaaagaa ttgtctttag
   122701 aggaaaaatt caaatcacat attcattttg aagagggcat ggatgattct ttgctctctt
   122761 tttatttaaa tatggcaaaa gattatgtca aaacagcaac tggtggccaa caagaatatc
   122821 ttattttgat ggttgccggc attgcctatg aatatagagt ttcagaggat gaactcgaca
   122881 aagctatgaa tgccatgacg ccatttatcg tgcaaggagc gattcaaaat gccgaagaga
   122941 cagaccaata atctcagatg gaaagccgaa ttgctagaca tcaaaacagg aacagacgga
   123001 aacgatcgtc caactacaat ttacgaattt aagcgtctaa tattctatga agaactcggt
   123061 gtgacttctc aagaaaaata tttatcacag caagccaaga cagacgttgt cagacgaatt
   123121 aaagtcagat gggataaatc catcacagag aaattaagtg cgctcaaaat tgattctgta
   123181 acgtataaca ttactcgcat ttatacgaat cccgatgcaa gagaaatgga gttgagttta
   123241 gcttatgtcg attagctttg atgaattgaa aacagcgctg aaatcaacaa agttagcagt
   123301 gttcagagac aaagccagat tagggacgat gtatccatac attgtgtact caaatgtgag
   123361 taacagcaaa aaaatggcat ccggtaaagt ctataaaaaa ttaccttatt accaagtctc
   123421 tttttttaca cttggaacag aacaggattt agttgttatt gaacaagcgc tgcaaaatgc
   123481 tggcattcca tattcagatt ttacaggtat acaaggtgat gagaacgacg ataccgtaac
   123541 caactattac acatatgtga ggtgtgtgga aaatgccaag taataagaat ggtttttctg
   123601 aaatatcgga ttatttagga aacctttcaa aagttgatcc gaaaaaatta tcgttggaat
   123661 ctttagaaga agcagctaac ttttatctaa agcagttgct tcctaatatt cctaagtcgc
   123721 tcttaaaaaa gaaacatatg cgcgatcaaa taaaagtagt agttgaaaac gatcaagtga
   123781 aagttcaatt tgaagagacg gccttttact ggcgctttgc cgaaaatggc acaacaaatc
   123841 aacgtgccca acattttgca agtggtacgt atgaacaaaa caaagaaaaa attgaagaaa
   123901 ttatgactag aaaaatactt gatttatggg aagggtgatt ttaattggga aaacaagata
   123961 ctttttattt tgaaggattg gacgacattt taattgcgat gatggcaacg ccagattctg
   124021 ttggaactgc accgacttat agtgaagttt tacgattgcc aattgccaca aaaatcggtg
   124081 tcaaagggaa tggaacagca ttagaaaaat gggcatccag caaaatgttt cgtcgtgtgt
   124141 cgcgagaaac aaaacatgaa cttgcgttgg atcacgtggg tattccgata gaagtgatgg
   124201 atgaaatcaa aggattagtt gctaaaagcg gagtaacgtt tagcaaaaat accgcacgag
   124261 aatttccgta cttcgccttt ggatttattg gaaatattga aaatggcggt aaaaaagcag
   124321 tttggtatcc taatacacaa ctatccaatg tgattgacga agagtatgcc actgcagagg
   124381 aagaaacaaa aattgacgat gtaactgcaa atctggtttc catcggactg aaatacaata
   124441 atgtgatgta tgcaagtttt gattcaaata gagacggagc ttctctagaa ttatttaata
   124501 aattcattgc tcaacctgta tacgatgaag aacagtggaa aacattggct aaagttggag
   124561 gtgcgggtta atggctcggt tatctgatta tggaattcac gttgaagact taaaaaattc
   124621 tgctgttgta attattcaag gcgcagaatt ccctatttca tttactatgc aaacgatgga
   124681 atttatagca gatgtgtatg gtggagatta ttcgcaattt gaatctgata tgaacgccat
   124741 gctatctaaa aaagaaggac aaatttcttc tgctaactta tcgcctagtg atctaaaaat
   124801 catgcgtgcc ttgatttatg ccatgctgcg tactggtggt ttagaggaag atccagaaac
   124861 tattttcaaa tttttgggaa tgagtggaga ggtgttgtct gcttatagta cctgtatgga
   124921 aatttttgct agccagacat ttcaggtgga agacctaaaa aaatccaaga agccacaaga
   124981 ctttcaaaaa gcgcaagcaa aaagaaagaa aaacaaaaag aatcggaaga gataggaact
   125041 ccttggagtt tttatattta cgttgctctc actctattga attggagtga gagtttcttt
   125101 ttaaagtcta cacctaactt gtggctcaaa tcttatttac agtggttaca acaaaacacc
   125161 gattttgaac cacctcaatc tgtaactatg gataaatcgc cttggtggta gaaaggagcg
   125221 ctaagataaa tggctggtaa agaatctgat gtcgttctta attttaaaac gaatggcgaa
   125281 gtcagttatt cgaaaacaat caaagaaatc aacaaagaaa tgaacttagc cgctgccgag
   125341 tacaaaaacc aagtgtctgc gatggacaag gatgcaactc aaacagaaaa attgcgagcg
   125401 gctaaacaaa aattagaaaa acaattagga ttggctgaac aacgatctca aatgctgaga
   125461 gaggaatatg aaaaatctgt caaagaaacc ggtgaatatt ctgctgaatc agaaaaactt
   125521 tataaacaat tactcaattc tgaaaccgga gaaaataaac ttcgtacagc attagaacag
   125581 acgaacgatg cacttaaaga acaaggtgac gtttctgttg atacagcaaa aaaactccag
   125641 aaaatcgaag aaacaggtga gaaagtaaaa ggcgttggtg aaaaaatgtc tgttggagta
   125701 accgcgccta ttgtagcggc aggagcagca ggacttgcag catttggtga agttgacgag
   125761 gcacttgata ccatcattac aaaaaccgga gcaacaggtg atcaagctga tagactttca
   125821 cagtctttca aaaacgttgg ttcaaatact catttacctt tacaaacggt tggggaagct
   125881 attggtgagg taaatacaca atttggattc atggataaaa aactggaaga ttcaaccaat
   125941 tatctcctac agtacgctga aatcaatgat acagatgttt cgcaatcagc aatatctgct
   126001 cgacaagcta ttgatgctta tggactagaa tatgatgatt tgaattctgt ccttgatgta
   126061 acaacgaaaa catcgcagaa tactggtcaa tctgtagacg acttgatgca aaaagcaatt
   126121 gatggcgcac cacaaattaa acaattaggg ttgagctttg gtgaaggggt cactttactt
   126181 ggacaatttg agcaatctgg tgttgattca agcgcagctt taagtagttt atctaaagca
   126241 acagtggctt atgcgaaaga cggaaaatct ttaagtcaag gactagaaga actacaagac
   126301 aaggtaaaaa atgcaggttc tgaaacagaa gccatcaatg cagcagcaga agtattcggg
   126361 actaaaggcg gtcctagaat ggccgatgca attcgtagag gtactttgaa cctggaagat
   126421 ttagcaaaaa ctgctggaga aagtggggga gctgtaggag acacattcga tgctactctc
   126481 gacccaattg atcaagccga tcaagctatg aacaatgcaa aattagcaat ggctgacgta
   126541 ggtgaagcag tccaagtaag ccttttgcct ttttttgaaa aagcaacttc cttattacag
   126601 gaattctcta aatggtgggg gtctctagat caagatacaa agaattggat cataacaatt
   126661 gctggtatag cggcagcagt tggaccagta ctaatcgttc ttggttcact tatgggatct
   126721 gttacaaaaa tcgttgggga agttaaaagt tttataggag tttggcaagg acttgctggt
   126781 ttatttggaa tgtcgggagg ctggtttgca ttagcagtta tagctattgg cgcattagtt
   126841 actggactaa tttgggcata taataacgtt aaatggtttc atgatggagt gaattctttc
   126901 ttcaaaggtg tttcagatgt agctgttgaa atatttaatt ttttaagtgg ttattttggt
   126961 ggatttttcg atggtgttat agcaaatttc aataattttt ataatgctgg aaaacgaatt
   127021 ttcagtggtt ttctagattt tatcactgga gtatttacag ggaattggtc aaaagcatgg
   127081 caaggtctag ttgatatttt tggaggtatt ttcgatggta ttgttgctgt agggaaagct
   127141 cctattaatg ccatgattgg attgatcaat ggatttattg gtggattgaa taatataaag
   127201 ataccaaaat gggttcctgg tgtaggtgga aaatcatttt ctatttcaaa actgccttat
   127261 ttagcacaag gcggtcatct aatcaatgga caagcgattg ttggtgaagc tggacctgag
   127321 ttactgactg ccaagaatgg taaaaccaca gttactccat tatcagacga agaaaaacgc
   127381 aaagggatca gtggaagagt ttctggaggt agcattgaac agcatataca cattggcaaa
   127441 gtcgatgcaa acaatccatc tgagttagat cgtatgaatc gtaaatttgc taaagcaaac
   127501 aaacaggcta ttcgtgattt gggaggtgtt cctatttgag tcgacaattt atgaatccag
   127561 atgaaccaaa ttttatttgg aaaaatcgga atgcagtaat tgatatgaat tgcatcattg
   127621 aggcagagct tccagatatt atgccgaaca agcgatacga aacttacacg attcagggcc
   127681 gaagcggaga attgacggag acgtttgatg actatgaacc ttttgatttg gaaatagaag
   127741 ggatcactat tccacattcg aaactgagag aagtcaaacg atggctcact ggtaaaagcc
   127801 aattaatcac acataatgat ccagacaagt atctagaggc tatctgcagc atggataaag
   127861 aagtcccttt cgaaaacgag tggggcttct tttatacatt cgatgttact tttcgttgtc
   127921 agccactcaa aagaaaaatc ggtgaagcac ctaaaatttt tcattctagt gttatggata
   127981 tttttgatca tggtgacgaa attgcacatc cctatctgga aatagaatcg aacggaggag
   128041 atataacgat cgctgtcggt gataaaagcc taacaatact taatacgtta gctgcaacaa
   128101 tcgtggttga tactcagctt ggaaaagcaa tacaagaagg tttgaattta tttacgcgtg
   128161 gtgattggcc agtattgcag cctgaatgga atcaggtaaa aatatccgga aaatttaaag
   128221 aagtgcggtt ttggaatagg agtgtgtatc tgtgacgcaa gaatttattt atgcttataa
   128281 aaaaatgcca gatgatttga gcgtgaacgg agcgtcgtta gttgattggg aagatttgcc
   128341 agaaatcaat cgtgtgctga atggccaata tcgtttttat ggtaactatt caagaagcgg
   128401 acagtatcgc tcttatttaa aaaaaggaaa ttttataaaa gcgaaggtac cagatggatc
   128461 atggcaatat tttgaaattt ataatgtcaa aaagaatttg acctccgttt ctgtaacagc
   128521 tcggcatatt ggttttatgg ccaataagaa ctttattgtc aagtcgttta cggataacgg
   128581 caatgggtct cagatcatga ccaatcttaa aaacagtttg gcatttgatc aaaaatttaa
   128641 ctatctttcg aatgtaggga caacgcacca atttacagca agacaagtcg cgccagttga
   128701 agcaatcatt ggttcaaata acggtaatca aaatttgact ggcgtgacta gcgcggaact
   128761 tgatatggat aactatgatt tgaaactggt aaaacagatt ggatcagata atggttttcg
   128821 gattgatttt ggcatcaatt tagaagcaat cgaagaagaa attgatgaag aatcaattgt
   128881 caatagtttg tatttggttg gcggtgttcc tgataacgat tatgatgaag acaaagaacc
   128941 tatcgaatat ggttatttag aaattgatgg agtaaccaac gagaatcgac gtattgccaa
   129001 acgtgaaaac ggagattgta aaacagttga tgaattgatc aaatggggca aaaccttatt
   129061 tgacaatgat cgtatccatg agccaaaagc tactcataca gtcagtatgg tggcgttgga
   129121 acacacactc gaatacggtg acatgtatca agagctagcg tctttacact ttggtgatgt
   129181 cgctcatgtt agggcgaaag agctagatat cgagataaaa gagcgtatgg tcgaatacac
   129241 ttatttccca acgcttggga aatataagga tattgtttta ggtaatgatt tatcactgta
   129301 cacttcggca gtaaatactc aagcacaaga attgaaaaag aaaatcgaca atcgaacaga
   129361 aacgttagtt caaaatgtgt tgaatgcgac ggcatggatc actggtaatt ctggtgggca
   129421 tgtcgttttt cgtccagaaa aagctccatc agagattctt atcatggata ctccaaatgt
   129481 ggcgaatgca aagcgagtgt ggcgttggaa tttaaatggc ttaggttatt cagacaacgg
   129541 agtaaatggc ccgttcggta ttgctatcac atcaaaagga gaaattgttg ctgacttcat
   129601 caaagtagga acaataaatg cagaagtatt cgaaacttcc tttaatgctt atggtgatgt
   129661 gttaaaactt gtaaaaggca cgcttcaaat ttggaatgaa aacaaaaaaa tcatggagct
   129721 aaccaaaaaa ggtatggaat tctggaatag aaaagaatcc attggaacga tcggaacaac
   129781 agattcagca ggaaatccat ttccaaatgc tgtaacacct acacctcttg aggaaaattc
   129841 tttggtaata cgtacaaatg gcgatggaaa atacatttta atttctccaa ctgcagaaaa
   129901 aggatttgtg ttattaggaa acggaaaagc atattatttc ggtgatttag atattcaggg
   129961 aaaacttact gttagaggta aagaagtgat accaggacaa aatggcggtc catctggcgg
   130021 aggagaaaca cctggtgggt atcctgatga attaaaaaca gatgcagaaa agagagcttg
   130081 gagaat
//
LOCUS       DAZKMZ010000002       126470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000002 DAZKMZ010000000
VERSION     DAZKMZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 126470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 126470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.002"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..997
                     /locus_tag="ACG8M5_000155"
     CDS             116..997
                     /locus_tag="ACG8M5_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0357445.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1010..2620
                     /locus_tag="ACG8M5_000156"
     CDS             1010..2620
                     /locus_tag="ACG8M5_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357446.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2697..3356)
                     /locus_tag="ACG8M5_000157"
     CDS             complement(2697..3356)
                     /locus_tag="ACG8M5_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0357447.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3639..4304)
                     /locus_tag="ACG8M5_000158"
     CDS             complement(3639..4304)
                     /locus_tag="ACG8M5_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0357448.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4451..5056)
                     /gene="dhaL"
                     /locus_tag="ACG8M5_000159"
     CDS             complement(4451..5056)
                     /gene="dhaL"
                     /locus_tag="ACG8M5_000159"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0357449.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5084..6094)
                     /gene="dhaK"
                     /locus_tag="ACG8M5_000160"
     CDS             complement(5084..6094)
                     /gene="dhaK"
                     /locus_tag="ACG8M5_000160"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0357450.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6069..6464)
                     /gene="dhaM"
                     /locus_tag="ACG8M5_000161"
     CDS             complement(6069..6464)
                     /gene="dhaM"
                     /locus_tag="ACG8M5_000161"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0357451.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6618..6938
                     /locus_tag="ACG8M5_000162"
     CDS             6618..6938
                     /locus_tag="ACG8M5_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0357452.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7050..7907)
                     /locus_tag="ACG8M5_000163"
     CDS             complement(7050..7907)
                     /locus_tag="ACG8M5_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0357453.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(8015..9319)
                     /locus_tag="ACG8M5_000164"
     CDS             complement(8015..9319)
                     /locus_tag="ACG8M5_000164"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0357454.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9316..9825)
                     /locus_tag="ACG8M5_000165"
     CDS             complement(9316..9825)
                     /locus_tag="ACG8M5_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357455.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10000..12135)
                     /locus_tag="ACG8M5_000166"
     CDS             complement(10000..12135)
                     /locus_tag="ACG8M5_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0357456.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12218..13873)
                     /locus_tag="ACG8M5_000167"
     CDS             complement(12218..13873)
                     /locus_tag="ACG8M5_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0357457.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13875..14189)
                     /locus_tag="ACG8M5_000168"
     CDS             complement(13875..14189)
                     /locus_tag="ACG8M5_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0357458.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14359..14619
                     /locus_tag="ACG8M5_000169"
     CDS             14359..14619
                     /locus_tag="ACG8M5_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0357459.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14633..14947
                     /locus_tag="ACG8M5_000170"
     CDS             14633..14947
                     /locus_tag="ACG8M5_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0357460.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15677..16231)
                     /locus_tag="ACG8M5_000171"
     CDS             complement(15677..16231)
                     /locus_tag="ACG8M5_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0357461.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16357..18286)
                     /locus_tag="ACG8M5_000172"
                     /pseudo
     CDS             complement(16357..18286)
                     /locus_tag="ACG8M5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18592..19308)
                     /locus_tag="ACG8M5_000173"
     CDS             complement(18592..19308)
                     /locus_tag="ACG8M5_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0357462.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19309..21132)
                     /gene="glpO"
                     /locus_tag="ACG8M5_000174"
     CDS             complement(19309..21132)
                     /gene="glpO"
                     /locus_tag="ACG8M5_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0357463.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21146..22642)
                     /gene="glpK"
                     /locus_tag="ACG8M5_000175"
     CDS             complement(21146..22642)
                     /gene="glpK"
                     /locus_tag="ACG8M5_000175"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0357464.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22778..24238)
                     /locus_tag="ACG8M5_000176"
     CDS             complement(22778..24238)
                     /locus_tag="ACG8M5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357465.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24228..25523)
                     /locus_tag="ACG8M5_000177"
     CDS             complement(24228..25523)
                     /locus_tag="ACG8M5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0357466.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25855..26532)
                     /locus_tag="ACG8M5_000178"
     CDS             complement(25855..26532)
                     /locus_tag="ACG8M5_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0357467.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26562..27653)
                     /locus_tag="ACG8M5_000179"
     CDS             complement(26562..27653)
                     /locus_tag="ACG8M5_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0357468.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27754..28188)
                     /locus_tag="ACG8M5_000180"
     CDS             complement(27754..28188)
                     /locus_tag="ACG8M5_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0357469.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28772..30229)
                     /locus_tag="ACG8M5_000181"
     CDS             complement(28772..30229)
                     /locus_tag="ACG8M5_000181"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0357470.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30226..32121)
                     /locus_tag="ACG8M5_000182"
     CDS             complement(30226..32121)
                     /locus_tag="ACG8M5_000182"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0357471.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32179..33954)
                     /locus_tag="ACG8M5_000183"
     CDS             complement(32179..33954)
                     /locus_tag="ACG8M5_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0357472.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34004..35500)
                     /locus_tag="ACG8M5_000184"
     CDS             complement(34004..35500)
                     /locus_tag="ACG8M5_000184"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0357473.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35694..37316)
                     /locus_tag="ACG8M5_000185"
     CDS             complement(35694..37316)
                     /locus_tag="ACG8M5_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0357474.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37644..38957)
                     /locus_tag="ACG8M5_000186"
     CDS             complement(37644..38957)
                     /locus_tag="ACG8M5_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0357475.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39202..39426)
                     /locus_tag="ACG8M5_000187"
     CDS             complement(39202..39426)
                     /locus_tag="ACG8M5_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357476.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39446..39868)
                     /locus_tag="ACG8M5_000188"
     CDS             complement(39446..39868)
                     /locus_tag="ACG8M5_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0357477.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40010..40720)
                     /locus_tag="ACG8M5_000189"
     CDS             complement(40010..40720)
                     /locus_tag="ACG8M5_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0357478.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40738..41235)
                     /locus_tag="ACG8M5_000190"
     CDS             complement(40738..41235)
                     /locus_tag="ACG8M5_000190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357479.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41272..41724)
                     /locus_tag="ACG8M5_000191"
     CDS             complement(41272..41724)
                     /locus_tag="ACG8M5_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0357480.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41859..42464)
                     /locus_tag="ACG8M5_000192"
     CDS             complement(41859..42464)
                     /locus_tag="ACG8M5_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0357481.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42914..43534)
                     /locus_tag="ACG8M5_000193"
     CDS             complement(42914..43534)
                     /locus_tag="ACG8M5_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0357482.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43536..44405)
                     /locus_tag="ACG8M5_000194"
     CDS             complement(43536..44405)
                     /locus_tag="ACG8M5_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0357483.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44428..44835)
                     /locus_tag="ACG8M5_000195"
     CDS             complement(44428..44835)
                     /locus_tag="ACG8M5_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0357484.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44854..46143)
                     /locus_tag="ACG8M5_000196"
     CDS             complement(44854..46143)
                     /locus_tag="ACG8M5_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0357485.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46201..47540
                     /locus_tag="ACG8M5_000197"
     CDS             join(46201..46642,46642..47540)
                     /locus_tag="ACG8M5_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0357486.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYRVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            47609..48211
                     /locus_tag="ACG8M5_000198"
     CDS             47609..48211
                     /locus_tag="ACG8M5_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0357487.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            48233..48721
                     /locus_tag="ACG8M5_000199"
     CDS             48233..48721
                     /locus_tag="ACG8M5_000199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357488.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            49043..50416
                     /locus_tag="ACG8M5_000200"
     CDS             49043..50416
                     /locus_tag="ACG8M5_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357489.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            50400..50837
                     /locus_tag="ACG8M5_000201"
     CDS             50400..50837
                     /locus_tag="ACG8M5_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0357490.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(51117..52862)
                     /locus_tag="ACG8M5_000202"
     CDS             complement(51117..52862)
                     /locus_tag="ACG8M5_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0357491.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            53058..54134
                     /locus_tag="ACG8M5_000203"
     CDS             53058..54134
                     /locus_tag="ACG8M5_000203"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0357492.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54287..55639
                     /gene="murF"
                     /locus_tag="ACG8M5_000204"
     CDS             54287..55639
                     /gene="murF"
                     /locus_tag="ACG8M5_000204"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0357493.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            55961..57472
                     /locus_tag="ACG8M5_000205"
     CDS             55961..57472
                     /locus_tag="ACG8M5_000205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0357494.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            57602..57952
                     /gene="acpS"
                     /locus_tag="ACG8M5_000206"
     CDS             57602..57952
                     /gene="acpS"
                     /locus_tag="ACG8M5_000206"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0357495.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            57968..59089
                     /gene="alr"
                     /locus_tag="ACG8M5_000207"
     CDS             57968..59089
                     /gene="alr"
                     /locus_tag="ACG8M5_000207"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0357496.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            59100..59462
                     /locus_tag="ACG8M5_000208"
     CDS             59100..59462
                     /locus_tag="ACG8M5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0357497.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(59638..59907)
                     /gene="rpsN"
                     /locus_tag="ACG8M5_000209"
     CDS             complement(59638..59907)
                     /gene="rpsN"
                     /locus_tag="ACG8M5_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0357498.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            60097..61044
                     /locus_tag="ACG8M5_000210"
     CDS             60097..61044
                     /locus_tag="ACG8M5_000210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0357499.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            61350..64055
                     /locus_tag="ACG8M5_000211"
     CDS             61350..64055
                     /locus_tag="ACG8M5_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0357500.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            64653..66476
                     /locus_tag="ACG8M5_000212"
     CDS             64653..66476
                     /locus_tag="ACG8M5_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0357501.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(66618..66758)
                     /locus_tag="ACG8M5_000213"
                     /pseudo
     CDS             complement(66618..66758)
                     /locus_tag="ACG8M5_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            67279..67542
                     /locus_tag="ACG8M5_000214"
     CDS             67279..67542
                     /locus_tag="ACG8M5_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0357502.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            67542..67808
                     /locus_tag="ACG8M5_000215"
     CDS             67542..67808
                     /locus_tag="ACG8M5_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0357503.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            68009..68497
                     /locus_tag="ACG8M5_000216"
     CDS             68009..68497
                     /locus_tag="ACG8M5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0357504.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(69038..69709)
                     /locus_tag="ACG8M5_000217"
     CDS             complement(69038..69709)
                     /locus_tag="ACG8M5_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357505.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(69706..70623)
                     /locus_tag="ACG8M5_000218"
     CDS             complement(69706..70623)
                     /locus_tag="ACG8M5_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0357506.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(70620..71261)
                     /locus_tag="ACG8M5_000219"
     CDS             complement(70620..71261)
                     /locus_tag="ACG8M5_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357507.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(71265..72439)
                     /locus_tag="ACG8M5_000220"
                     /pseudo
     CDS             complement(71265..72439)
                     /locus_tag="ACG8M5_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            72632..73663
                     /gene="queA"
                     /locus_tag="ACG8M5_000221"
     CDS             72632..73663
                     /gene="queA"
                     /locus_tag="ACG8M5_000221"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0357508.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            74282..76921
                     /locus_tag="ACG8M5_000222"
     CDS             74282..76921
                     /locus_tag="ACG8M5_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0357509.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(77089..77787)
                     /locus_tag="ACG8M5_000223"
     CDS             complement(77089..77787)
                     /locus_tag="ACG8M5_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0357510.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            78005..79150
                     /gene="tgt"
                     /locus_tag="ACG8M5_000224"
     CDS             78005..79150
                     /gene="tgt"
                     /locus_tag="ACG8M5_000224"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0357511.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            79247..79627
                     /gene="yajC"
                     /locus_tag="ACG8M5_000225"
     CDS             79247..79627
                     /gene="yajC"
                     /locus_tag="ACG8M5_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0357512.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            79932..82529
                     /gene="adhE"
                     /locus_tag="ACG8M5_000226"
                     /gene_synonym="adhC"
     CDS             79932..82529
                     /gene="adhE"
                     /locus_tag="ACG8M5_000226"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0357513.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            82758..83864
                     /locus_tag="ACG8M5_000227"
     CDS             82758..83864
                     /locus_tag="ACG8M5_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0357514.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            83881..84909
                     /locus_tag="ACG8M5_000228"
     CDS             83881..84909
                     /locus_tag="ACG8M5_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0357515.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            84915..85394
                     /locus_tag="ACG8M5_000229"
     CDS             84915..85394
                     /locus_tag="ACG8M5_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0357516.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            85410..86705
                     /locus_tag="ACG8M5_000230"
     CDS             85410..86705
                     /locus_tag="ACG8M5_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0357517.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            86716..87540
                     /locus_tag="ACG8M5_000231"
     CDS             86716..87540
                     /locus_tag="ACG8M5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0357518.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            87705..88604
                     /locus_tag="ACG8M5_000232"
     CDS             87705..88604
                     /locus_tag="ACG8M5_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357519.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(88730..89599)
                     /locus_tag="ACG8M5_000233"
     CDS             complement(88730..89599)
                     /locus_tag="ACG8M5_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0357520.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            89752..91212
                     /gene="rhaB"
                     /locus_tag="ACG8M5_000234"
     CDS             89752..91212
                     /gene="rhaB"
                     /locus_tag="ACG8M5_000234"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0357521.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            91209..92465
                     /gene="rhaA"
                     /locus_tag="ACG8M5_000235"
     CDS             91209..92465
                     /gene="rhaA"
                     /locus_tag="ACG8M5_000235"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0357522.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            92479..93324
                     /gene="rhaD"
                     /locus_tag="ACG8M5_000236"
     CDS             92479..93324
                     /gene="rhaD"
                     /locus_tag="ACG8M5_000236"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0357523.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            93326..93637
                     /gene="rhaM"
                     /locus_tag="ACG8M5_000237"
     CDS             93326..93637
                     /gene="rhaM"
                     /locus_tag="ACG8M5_000237"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0357524.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            93719..94870
                     /gene="fucO"
                     /locus_tag="ACG8M5_000238"
     CDS             93719..94870
                     /gene="fucO"
                     /locus_tag="ACG8M5_000238"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0357525.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            94997..95755
                     /locus_tag="ACG8M5_000239"
     CDS             94997..95755
                     /locus_tag="ACG8M5_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0357526.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            95855..96499
                     /locus_tag="ACG8M5_000240"
     CDS             95855..96499
                     /locus_tag="ACG8M5_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0357527.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            96506..97513
                     /locus_tag="ACG8M5_000241"
     CDS             96506..97513
                     /locus_tag="ACG8M5_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0357528.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            97529..98371
                     /gene="kduI"
                     /locus_tag="ACG8M5_000242"
     CDS             97529..98371
                     /gene="kduI"
                     /locus_tag="ACG8M5_000242"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0357529.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            98391..99194
                     /locus_tag="ACG8M5_000243"
     CDS             98391..99194
                     /locus_tag="ACG8M5_000243"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0357530.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(99443..100507)
                     /gene="fni"
                     /locus_tag="ACG8M5_000244"
     CDS             complement(99443..100507)
                     /gene="fni"
                     /locus_tag="ACG8M5_000244"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0357531.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(100504..101589)
                     /locus_tag="ACG8M5_000245"
     CDS             complement(100504..101589)
                     /locus_tag="ACG8M5_000245"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0357532.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(101602..102579)
                     /gene="mvaD"
                     /locus_tag="ACG8M5_000246"
     CDS             complement(101602..102579)
                     /gene="mvaD"
                     /locus_tag="ACG8M5_000246"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0357533.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(102572..103516)
                     /gene="mvk"
                     /locus_tag="ACG8M5_000247"
     CDS             complement(102572..103516)
                     /gene="mvk"
                     /locus_tag="ACG8M5_000247"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0357534.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            103832..104491
                     /locus_tag="ACG8M5_000248"
     CDS             103832..104491
                     /locus_tag="ACG8M5_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0357535.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            104576..105481
                     /locus_tag="ACG8M5_000249"
     CDS             104576..105481
                     /locus_tag="ACG8M5_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0357536.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(105483..106115)
                     /locus_tag="ACG8M5_000250"
     CDS             complement(105483..106115)
                     /locus_tag="ACG8M5_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0357537.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    106266..106391
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            106435..106959
                     /gene="infC"
                     /locus_tag="ACG8M5_000251"
     CDS             106435..106959
                     /gene="infC"
                     /locus_tag="ACG8M5_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0357538.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            107031..107231
                     /gene="rpmI"
                     /locus_tag="ACG8M5_000252"
     CDS             107031..107231
                     /gene="rpmI"
                     /locus_tag="ACG8M5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0357539.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            107284..107643
                     /gene="rplT"
                     /locus_tag="ACG8M5_000253"
     CDS             107284..107643
                     /gene="rplT"
                     /locus_tag="ACG8M5_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0357540.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            107895..109232
                     /locus_tag="ACG8M5_000254"
     CDS             107895..109232
                     /locus_tag="ACG8M5_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0357541.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            109280..111409
                     /locus_tag="ACG8M5_000255"
     CDS             109280..111409
                     /locus_tag="ACG8M5_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0357542.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            111384..112073
                     /locus_tag="ACG8M5_000256"
     CDS             111384..112073
                     /locus_tag="ACG8M5_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357543.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(112481..113167)
                     /locus_tag="ACG8M5_000257"
     CDS             complement(112481..113167)
                     /locus_tag="ACG8M5_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0357544.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(113303..113497)
                     /locus_tag="ACG8M5_000258"
     CDS             complement(113303..113497)
                     /locus_tag="ACG8M5_000258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357545.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(113547..113987)
                     /locus_tag="ACG8M5_000259"
     CDS             complement(113547..113987)
                     /locus_tag="ACG8M5_000259"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0357546.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(113991..114836)
                     /locus_tag="ACG8M5_000260"
     CDS             complement(113991..114836)
                     /locus_tag="ACG8M5_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0357547.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            114985..116403
                     /gene="pepV"
                     /locus_tag="ACG8M5_000261"
     CDS             114985..116403
                     /gene="pepV"
                     /locus_tag="ACG8M5_000261"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0357548.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(116460..117407)
                     /locus_tag="ACG8M5_000262"
     CDS             complement(116460..117407)
                     /locus_tag="ACG8M5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0357549.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(117630..117980)
                     /locus_tag="ACG8M5_000263"
     CDS             complement(117630..117980)
                     /locus_tag="ACG8M5_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0357550.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            118180..119259
                     /gene="pepA"
                     /locus_tag="ACG8M5_000264"
     CDS             118180..119259
                     /gene="pepA"
                     /locus_tag="ACG8M5_000264"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0357551.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            119403..119723
                     /locus_tag="ACG8M5_000265"
     CDS             119403..119723
                     /locus_tag="ACG8M5_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0357552.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            119745..120209
                     /locus_tag="ACG8M5_000266"
     CDS             119745..120209
                     /locus_tag="ACG8M5_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0357553.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            120414..121019
                     /gene="ytpR"
                     /locus_tag="ACG8M5_000267"
     CDS             120414..121019
                     /gene="ytpR"
                     /locus_tag="ACG8M5_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0357554.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(121058..122347)
                     /locus_tag="ACG8M5_000268"
     CDS             complement(121058..122347)
                     /locus_tag="ACG8M5_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0357555.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            122775..123254
                     /gene="rlmH"
                     /locus_tag="ACG8M5_000269"
     CDS             122775..123254
                     /gene="rlmH"
                     /locus_tag="ACG8M5_000269"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0357556.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            123577..125212
                     /locus_tag="ACG8M5_000270"
                     /pseudo
     CDS             123577..125212
                     /locus_tag="ACG8M5_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            125466..125780
                     /locus_tag="ACG8M5_000271"
                     /pseudo
     CDS             125466..125780
                     /locus_tag="ACG8M5_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(126085..>126470)
                     /locus_tag="ACG8M5_000272"
     CDS             complement(126085..>126470)
                     /locus_tag="ACG8M5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0357557.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT        38562 a        24392 c        24911 g        38605 t
ORIGIN      
        1 aagaatatta tgtactaaaa taataaaaaa agttttataa aaaggtataa aaccttttta
       61 ttaaactatt taatcttgtt tagtatatat ttttagaaaa aggagtgggg tttttatggc
      121 agaaatcgtc aaagtcacac atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga
      181 tgtctctttc accgtacatt caggtgaagt cgttggattt atcggaccaa atggttctgg
      241 aaaatccacg acgattcgga cacttttagg gattataaaa aaagacggcg gaacggcaac
      301 gatttttggg caagatgtat gggaagatag tttatctatc catgaacgtc tttcttatgt
      361 tccaggcgat atctctttgt ggggaaattt gactggagga gcgatcatcg atctcttcat
      421 gaagcttcat ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct
      481 tgatcctaag aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag tgggcttgat
      541 tgctgctttg gctgttgatt cggatctcta tttatttgat gagcctactt ctggtttaga
      601 tcctttgatg gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc
      661 catcctactt tcttctcata ttctcagtga agtcgagcga ctagcggata aagtcgtgat
      721 catacgcgaa ggaacactcg atgaagaagg aacactcgat gaattgcgtc atttgactcg
      781 ttcgaccatc actatagaaa cagaagcaga gaatagtcac ttagcacaat tgaacggtgt
      841 ccatgacttt gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg aagctttcga
      901 tgtgatttta gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac
      961 tcttgaggat ttattcatga gacattatga aggatgagga ggaagcgaaa tgactgataa
     1021 attttcacgc tggtccaccc ttttttctca aaaccttagc cgtgactgga aaaagatcct
     1081 gatatggcta gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt
     1141 gacttccgga caaggattag ctggattata cgaaaccttg caaaacccgg caatgattgc
     1201 aatggtcgga ccaactccag cttcgaatgc tgctgagtat actttaggag cgatgtatgc
     1261 aaatgaaatg ttgctttttt gcagtttgtt tgctgctgtg atctctgttt tacacgtggt
     1321 gagccaaacg agaaaaaaag aagaacttgg tctgattgaa cttattcgtt cctttcgagt
     1381 cggacgtcaa gcaaactctt tagctgtcat cgctgaaagt attctgatca atttgctttt
     1441 agcaatatcg atcagctgta cgatgatttt tttccaagcg gattcgatca ctgcagaagg
     1501 tgcttggctt tttgggctat ctgtaggtag tatggggatt ttggggagtg ccgttgcact
     1561 gtttgctgca caactgatgc caagttcagc cagttcgaat ggtctgtctt tagcgttttt
     1621 aggtggactt taccttctac gagcaataac agatgtagct gattcctccc tttcgatgtg
     1681 gaatccttta ggctggtctt atttgaccta tccatttaca actaacaact ggattccttt
     1741 gctttatttg tttttgttca gtctaatctt gataatcagt tcatttgttt tagaggaaca
     1801 tcgagatttg aacaccggct atatccccga acgtgaagga aaaccgtatg ctaaacgttc
     1861 tcttttatcc ttgcgtggtt tattattcta tctaaataaa gggatgacaa tcagttggct
     1921 tatcactttt ggtttgatgg gggcggcata cggctctatc tacgggaata tgcaacagtt
     1981 tttagaaagt aacgacttaa tgaaacagat gtttatccaa tctggcgtct caattgaaaa
     2041 aaactttaca agcacgatca tggtagtctt gatttgtcta gctgctattt taccaattgc
     2101 tagcatcaat cgtttattta cagaagaaac ccacggacgt atgaatcagc tgttcgcaac
     2161 aaaaatttct aggaaccaac tttattggac caatatgtta ctagctattc tatttgctat
     2221 attggcaatc ctgattagtt ctggtgcact agggctttct gctttggctt cgatgaagga
     2281 tgcgaaagac atggatctat tgatgtttct taaagccggg ttcaatttcc tgcctgccgt
     2341 tttagtgttt atcggaatta gtggctttct attcggatgg cttcctcgaa tcggtaaact
     2401 tgcttatatc tacttagctt actcctttgc actcaattac tttggcggaa ttttggattt
     2461 accagaatgg ttttccaaaa cagctgtccc tagctgggtt ccacggatgc caaacgattc
     2521 ttttgatctt tccgtgttcc ttacgttatc atgtatcagc cttgcactgt tcgtccttgg
     2581 ttctattggt tataggcgaa gagacttact agaaggataa taaaaaagca tgtacaattt
     2641 catgctagcc tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt ttttatttac
     2701 aagtcttcta actgctcttt atcaatgatc cagacttctc gattctttct ttttacataa
     2761 ccattctcct ctaataattt aaattttcta gataaagttt cgggggtcgt accgataaat
     2821 gcagcaagtt ctttcatttt cattggcagg aaaaagtgat ccttccctgt cgctttagct
     2881 aagtcaagca gatagacagc gactcgttct tctacttttt ccatcatcaa aaaagttgtc
     2941 tgctgctgta cttttgttaa tttctcaacg ttgattgcta aaagtttcag actaagggcg
     3001 ggataatgca gtaagagttc cgagaaatct ttttgtctca atatgcaaat ttccgttttt
     3061 tctagtgctt cgccaaataa agactcattt ggaacgccta gcaactggct ctctccctca
     3121 tatccgcccg gctctattac tcgtaaaagc tgttcctttc cgctagcaga taattgatac
     3181 actttcatac tgccggaggc cacgatcact aattgaggtt ctccattcgg cgcaagaatc
     3241 tgttctcctt tttccacata gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc
     3301 tgtactaagt gattgaacaa aggaacaaat gaaacacata aatgatcatg actcataatc
     3361 tctcctcctt ctaaacagct atttctaata aagaaacata acttccctta ttccaactga
     3421 taattttctt ttatcatagc ataaacgtca agcgaaccag ataccagcaa atagagtgga
     3481 tttctttttg tcccttgctt tattttcctt cttttgagac cttaagtaat tgattcctat
     3541 caatttttta aatatttact ttttgaataa tcaaaaacaa accttcactc gcaagccgag
     3601 agtaaggttt gtttttcgca ttgcttattt cgtgttgtct attgaaattt ttccgcacct
     3661 agcttttgat atagtggtgc tagttcttgt aaggtttcag cggttatttg ctcaacgtgt
     3721 tcgatttctt gcggatagaa gaatagttta cccatttgaa aggttcggtc tctcatcgct
     3781 tttttcaact cttcttgttt ctttgtatcg gacaaatagt cagacaaagc taagtggtcg
     3841 tcaagttcat gttctggttg cggccatatg taataatcct tgatatcgac tgttttgctc
     3901 cactctttca gtatcgcaag caactggtta tacgctgcaa tgctaccata tcgttcttcg
     3961 cttttataat gttggtacat caaatagatt tggtattgtt tttgattcaa caaaactgct
     4021 agacaggcat tctcgtcttg acgagattcg cttcggtacg cgctccaaaa atgactgcgt
     4081 aaattccagc cattcgtcca gctttcgatt ttaggtttgc tcatgccgta catatcaggc
     4141 aaaagtgcag ctgtctggag ctgaatctct ttccattttt gccagtgttc tttatattct
     4201 ttcttgattt gttcaatctt atctgttgga tggctctctt tgagctgttt gaatgcgtag
     4261 attggtcgat caaacaactg ataatctgat tttccaagtt ccattgtttc gcctctttcc
     4321 gttatctttt gtattctacc tgaattttgc gatatggaca aatagaggtt gtgaaaaagc
     4381 tgttctgcat caagtcataa gaacagccaa acaaaaaact gcgatgagca tcaccgcagt
     4441 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
     4501 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
     4561 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
     4621 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
     4681 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
     4741 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
     4801 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
     4861 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
     4921 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
     4981 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
     5041 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
     5101 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
     5161 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
     5221 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
     5281 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
     5341 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
     5401 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     5461 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     5521 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     5581 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     5641 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     5701 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     5761 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     5821 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     5881 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     5941 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     6001 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     6061 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     6121 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     6181 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     6241 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     6301 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     6361 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     6421 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     6481 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     6541 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     6601 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     6661 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     6721 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     6781 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     6841 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     6901 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     6961 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     7021 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     7081 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     7141 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     7201 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     7261 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     7321 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     7381 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     7441 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     7501 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     7561 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     7621 tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta
     7681 tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg
     7741 gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa
     7801 ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata
     7861 ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc
     7921 cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca
     7981 caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt
     8041 tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga
     8101 tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt
     8161 tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga
     8221 ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt
     8281 ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga
     8341 atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt
     8401 taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg
     8461 cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga
     8521 taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga
     8581 tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat
     8641 acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt
     8701 taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac
     8761 gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga
     8821 aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta
     8881 aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat
     8941 ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt
     9001 ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca
     9061 atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca
     9121 ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat
     9181 gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg
     9241 gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     9301 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     9361 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     9421 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     9481 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     9541 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     9601 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     9661 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     9721 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     9781 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     9841 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     9901 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     9961 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
    10021 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
    10081 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
    10141 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
    10201 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
    10261 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
    10321 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
    10381 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
    10441 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
    10501 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
    10561 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
    10621 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
    10681 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
    10741 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
    10801 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
    10861 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
    10921 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
    10981 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
    11041 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
    11101 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
    11161 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
    11221 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
    11281 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
    11341 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
    11401 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
    11461 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
    11521 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
    11581 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
    11641 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
    11701 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
    11761 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
    11821 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
    11881 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
    11941 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
    12001 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
    12061 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
    12121 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac
    12181 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
    12241 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
    12301 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
    12361 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
    12421 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
    12481 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
    12541 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
    12601 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
    12661 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
    12721 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
    12781 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
    12841 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
    12901 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
    12961 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
    13021 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
    13081 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
    13141 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
    13201 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
    13261 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
    13321 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
    13381 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
    13441 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
    13501 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
    13561 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
    13621 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
    13681 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
    13741 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
    13801 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
    13861 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
    13921 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
    13981 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
    14041 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
    14101 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
    14161 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
    14221 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
    14281 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
    14341 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
    14401 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    14461 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    14521 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    14581 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    14641 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    14701 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    14761 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    14821 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    14881 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    14941 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    15001 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    15061 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    15121 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    15181 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    15241 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    15301 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    15361 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    15421 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    15481 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    15541 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt
    15601 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    15661 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    15721 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    15781 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    15841 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    15901 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    15961 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    16021 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    16081 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    16141 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    16201 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    16261 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    16321 ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    16381 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    16441 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    16501 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    16561 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    16621 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    16681 acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    16741 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    16801 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca
    16861 actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt
    16921 catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc
    16981 cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg
    17041 ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag
    17101 cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt
    17161 caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa
    17221 cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt
    17281 tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat
    17341 ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt
    17401 ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa
    17461 agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg
    17521 cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta
    17581 acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc
    17641 ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag
    17701 gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt
    17761 tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt
    17821 gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt
    17881 tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca
    17941 acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa
    18001 gaaaatccgc atattcgatc aaatcggttg gctgcagaat caaccctttt ttgacttgtt
    18061 ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga
    18121 tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga
    18181 ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct
    18241 ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc
    18301 ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca
    18361 cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag
    18421 gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta
    18481 gataccagct acactcacaa aacatacccc attcttctag gtcttattta ttttccactt
    18541 aactatagca agacacattt tggttgtcaa actttagtat gttttataga tttacaccat
    18601 taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca
    18661 tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg
    18721 aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc
    18781 gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg
    18841 tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt
    18901 tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc
    18961 tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc
    19021 tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat
    19081 cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata
    19141 gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg
    19201 tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat
    19261 cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc
    19321 ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc
    19381 cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat
    19441 ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc
    19501 tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact
    19561 caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc
    19621 gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt
    19681 tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg
    19741 atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg
    19801 tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa
    19861 agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc
    19921 tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac
    19981 tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat
    20041 cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag
    20101 tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt
    20161 gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc
    20221 ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac
    20281 catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt
    20341 cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt
    20401 aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc
    20461 tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc
    20521 gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc
    20581 agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac
    20641 acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg
    20701 tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc
    20761 cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg
    20821 caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc
    20881 accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg
    20941 aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc
    21001 tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc
    21061 gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt
    21121 gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt
    21181 tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc
    21241 cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg
    21301 ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat
    21361 tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt
    21421 tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt
    21481 gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca
    21541 agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg
    21601 caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc
    21661 tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa
    21721 aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca
    21781 ataaattatt tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg
    21841 taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt
    21901 gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg
    21961 tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta
    22021 gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac
    22081 tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag
    22141 aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat
    22201 gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt
    22261 tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact
    22321 gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta
    22381 ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg
    22441 attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat
    22501 gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt
    22561 gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca
    22621 tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac
    22681 tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct
    22741 ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg
    22801 tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat
    22861 attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt
    22921 cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt
    22981 gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg
    23041 caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct
    23101 cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca
    23161 gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct
    23221 catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt
    23281 agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg
    23341 tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat
    23401 actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca
    23461 ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat
    23521 cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt
    23581 acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg
    23641 cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc
    23701 ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata
    23761 gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt
    23821 gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact
    23881 cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat
    23941 agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac
    24001 ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt
    24061 tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg
    24121 acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt
    24181 ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag
    24241 gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat
    24301 agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg
    24361 tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca
    24421 gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct
    24481 acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg
    24541 cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt
    24601 gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact
    24661 agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc
    24721 gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca
    24781 ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa
    24841 acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc
    24901 aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact
    24961 aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa
    25021 agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct
    25081 gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt
    25141 tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga
    25201 tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg
    25261 actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg
    25321 tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt
    25381 aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat
    25441 atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac
    25501 ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa
    25561 cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat
    25621 gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact
    25681 agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata
    25741 aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa
    25801 agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt
    25861 ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt
    25921 aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc
    25981 tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc
    26041 ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga
    26101 ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc
    26161 atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat
    26221 tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg
    26281 acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc
    26341 gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact
    26401 gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact
    26461 taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat
    26521 caatactttc attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc
    26581 aagaaattat ataatgggct tggcaaataa agctctttat atgctttttc aaaaggtttc
    26641 gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc
    26701 atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga
    26761 ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact
    26821 tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat
    26881 gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct
    26941 tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat
    27001 ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa
    27061 cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag
    27121 aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat
    27181 tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca
    27241 ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca
    27301 ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata
    27361 atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt
    27421 tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca
    27481 gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct
    27541 tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg
    27601 ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt
    27661 cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt
    27721 agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc
    27781 atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga
    27841 ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt
    27901 aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa
    27961 tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt
    28021 ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag
    28081 acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac
    28141 gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc
    28201 attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc
    28261 gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt
    28321 ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt
    28381 tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    28441 ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg
    28501 catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc
    28561 taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga
    28621 actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga
    28681 cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa
    28741 gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt gattggatga
    28801 cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt
    28861 catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga
    28921 cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt
    28981 ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc
    29041 catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt
    29101 gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc
    29161 cccattcact tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga
    29221 ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa
    29281 caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga
    29341 cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat
    29401 cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac
    29461 aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa
    29521 tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt
    29581 atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt
    29641 gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc
    29701 gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc
    29761 caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct
    29821 caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat
    29881 tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc
    29941 atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat
    30001 ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct
    30061 gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg
    30121 tatacacatc agacacagat aatccttttt catctacatc ccatgcacct tcacattgat
    30181 tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc
    30241 taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa
    30301 tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc
    30361 ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc
    30421 actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc
    30481 tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc
    30541 cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa
    30601 agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc
    30661 aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa
    30721 aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac
    30781 aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc
    30841 accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta
    30901 aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa
    30961 tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa
    31021 taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt
    31081 atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc
    31141 aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa
    31201 gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc
    31261 tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt
    31321 tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac
    31381 agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag
    31441 aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc
    31501 tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa
    31561 aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc
    31621 tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc
    31681 acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga
    31741 gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt
    31801 catttgtttg gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa
    31861 cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc
    31921 tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct
    31981 gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat
    32041 ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt
    32101 cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca
    32161 atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat
    32221 atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg
    32281 tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag
    32341 acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat
    32401 tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta
    32461 atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat
    32521 ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc
    32581 ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt
    32641 tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat
    32701 actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat
    32761 cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata
    32821 agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa
    32881 ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg
    32941 tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg
    33001 gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag
    33061 attcttctct atttctttgt ttttttcatc attaatcatc aaataattta actctaatag
    33121 tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa
    33181 cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa
    33241 aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc
    33301 tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg
    33361 catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt
    33421 ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag
    33481 tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat
    33541 cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg
    33601 catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc
    33661 catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga
    33721 ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc
    33781 gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc
    33841 ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg
    33901 gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga
    33961 aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc
    34021 tatattcaag gtttttacca ttagactgga taatttgttt ataatattca aaactatctt
    34081 ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat
    34141 atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc caccataagt
    34201 aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta
    34261 gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc
    34321 caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga
    34381 cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca
    34441 aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt
    34501 tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc
    34561 actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa
    34621 tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa
    34681 attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag
    34741 aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag
    34801 cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg
    34861 tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag
    34921 gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt
    34981 agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc
    35041 accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg
    35101 gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct
    35161 catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa
    35221 aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat
    35281 caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt
    35341 tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca
    35401 cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc
    35461 cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc
    35521 aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg
    35581 gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa
    35641 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    35701 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    35761 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    35821 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    35881 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    35941 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    36001 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    36061 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    36121 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    36181 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    36241 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    36301 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    36361 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    36421 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    36481 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    36541 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    36601 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    36661 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    36721 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    36781 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    36841 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    36901 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    36961 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    37021 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    37081 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    37141 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    37201 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    37261 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    37321 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    37381 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    37441 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    37501 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    37561 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    37621 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    37681 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    37741 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    37801 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    37861 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    37921 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    37981 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    38041 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    38101 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    38161 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    38221 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    38281 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    38341 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    38401 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    38461 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    38521 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    38581 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    38641 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    38701 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    38761 acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    38821 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    38881 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    38941 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    39001 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta
    39061 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
    39121 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    39181 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
    39241 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    39301 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    39361 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    39421 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    39481 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
    39541 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
    39601 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
    39661 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    39721 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    39781 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
    39841 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    39901 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    39961 ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg
    40021 cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga
    40081 ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg
    40141 attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac
    40201 gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa
    40261 gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc
    40321 ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag
    40381 acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt
    40441 gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg
    40501 tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa
    40561 ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt
    40621 cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa
    40681 atacttccca gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta
    40741 gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg
    40801 ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc
    40861 ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc
    40921 tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat
    40981 ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta
    41041 acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc
    41101 ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga
    41161 attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac
    41221 atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat
    41281 ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt
    41341 taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt
    41401 ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata
    41461 gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc
    41521 taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt
    41581 ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt
    41641 taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc
    41701 gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc
    41761 acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa
    41821 ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt
    41881 ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac
    41941 tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct
    42001 tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct
    42061 ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt
    42121 tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca
    42181 ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt
    42241 ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc
    42301 gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc
    42361 cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga
    42421 gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt
    42481 ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa
    42541 tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg
    42601 acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc
    42661 accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga
    42721 cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt
    42781 cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc
    42841 tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta
    42901 agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag
    42961 tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt
    43021 tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc
    43081 ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat
    43141 gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg
    43201 ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt
    43261 tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca
    43321 aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt
    43381 ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg
    43441 catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag
    43501 gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga
    43561 ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt
    43621 gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta
    43681 gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta
    43741 ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg
    43801 acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg
    43861 aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca
    43921 aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct
    43981 tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca
    44041 tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca
    44101 tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg
    44161 tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg
    44221 actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc
    44281 gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta
    44341 aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta
    44401 tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc
    44461 aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc
    44521 acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc
    44581 gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata
    44641 caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg
    44701 ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc
    44761 tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt
    44821 aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa
    44881 gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt
    44941 gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc
    45001 atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc
    45061 tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa
    45121 catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat
    45181 gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg
    45241 aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac
    45301 accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat
    45361 gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt
    45421 gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc
    45481 gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa
    45541 aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat
    45601 atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc
    45661 gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa
    45721 atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat
    45781 gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt
    45841 cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat
    45901 agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa
    45961 acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa
    46021 cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat
    46081 caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc
    46141 catgttttga tgtgcacccc aaaagttaga ctagaaatct aattaaaagg ggtgcttttt
    46201 ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt aaataacgaa
    46261 ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt tagacattgg
    46321 gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa aagctacact
    46381 acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc tatccaagaa
    46441 actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat agagcaatat
    46501 gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat tagtatgaat
    46561 tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga agttattcgt
    46621 ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga acaagatttg
    46681 agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt aaattgaatc
    46741 tattactttc aattgcacaa ttagcgaagt caacttatta ttacagggta aaaaaattag
    46801 ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta aaagaatcaa
    46861 gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga tatacaatca
    46921 atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa atccgaataa
    46981 agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg ttaaaacgaa
    47041 atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa ttcaagataa
    47101 aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa ataattagtt
    47161 atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa caagcaatta
    47221 aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg cagtatcaga
    47281 tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg tctagaaaag
    47341 gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa tcagagtttt
    47401 tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat gagtatatta
    47461 ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca gtagaatatc
    47521 ggaaacagtc cattaaatag ttcaactttt ggggttcagt tcataaggag gcagttttaa
    47581 taaatgaaaa aagatgtggg gaatatagat gtctaaaaaa ggatcagttt tacaaaaatg
    47641 tcttcagatc atgcccatta ttggtctgtt ttttttcttt gtgttgattc tttatgctta
    47701 tcatcatggg atttttcgat cgagcacgtc tttgcaagcg tttatccagc aatttggcaa
    47761 gtatgcagtg atgatatttg ttctgttgca agtgatccaa gtgatcattc ctattttgcc
    47821 gggagggatc tcctcagtag cgggcatgct gatgtttggc aatggatttg ggcttttata
    47881 tagttgtatt ggcctagtga ttggggaagc aatcggattt ttgcttgtcc gttactatgg
    47941 cgtagccttt gtccagctta ttttatcgcc aaaaaagtat cagaaatttg atgaattgct
    48001 gacaagaaaa acgaaagata ttaaaaaagt actagttgtt accttgctgc ttccctttgc
    48061 gccagacgac ttgatctgtc tagtagctgg tttgacgaaa ttatctttta gggaatttat
    48121 gcagattgtg attttcttga aaccgtggtc ggtgggtgtg tatagcatga tcatgctctt
    48181 tttattccat caagcgcaag gtcatttata atgtaaaaaa ggacgtgggt gtatggaaaa
    48241 aataaaagta acagaaaatg aactggatga gctgatggca gtcatacaag aagtatggcc
    48301 ggaagttttt gtgccgatca ttggtcaaaa acaagtggat tacatgctga aaacttatca
    48361 gtcaaaaaaa cagattcaaa aagaactgac agaaggtgtg tcctactttt tattaaaaag
    48421 cgaagaaaag actgtcggtt acactgctta tgaagagata aatggaaaaa tttatttaag
    48481 taaattatat ttgctaaata aagtacgtgg tcaaggattg acttcttcag tttttcgctg
    48541 gtatgaagaa cttgctgcgc aaacaaagga aagagagatc tttttgcgag tgaatcaggg
    48601 gaatcatcaa gcaatcgagg tttataagca tgaaggattt gagattacag aagaacttat
    48661 ttcagatatc ggtcaaggat ttcaaatggt cgattataaa atgaaaaaag ctctcgcata
    48721 aacataggaa ctgtgttaaa gtaaatttat gcgacgggtc gagaaggact ctggtttaca
    48781 gagctcctaa ttgattttgg aggcacagga taattgattt ctatctttat cacctgccgg
    48841 atgcaggggt gtcgggcaag acttttgtgg agaagggctt gtctgcttga gtaatcaagt
    48901 accgtaaaaa tgcagaaaga agcttatgct tctttctgta ttttttttta caagaatata
    48961 ttctgtaaaa ttggtttatt tcccctttaa tgctaaaatc gaagagcaga ttagttataa
    49021 gactgtttaa ggagccattg tcatgttcga aaaactcaaa cgatttgggc gtaattttac
    49081 acaaacaacg ccctttatct taagtaacac ggttattttt attccttatc ttttattttt
    49141 gggactaaca gactcgcata attggctgac gattttgcca tttacgctgt tttatgcctt
    49201 tcggatgact ggactatttt tagtcaacag tctacgattt ggtctagata gttacacgtt
    49261 gctgatgatc gctctgctga ttggtggtgc tggttcattg cttggaatat taggcgtatt
    49321 ttattttcct ttattttatt tttcaagtgt tttgctaggg ctgagtgcag catggcttgt
    49381 acctgctaat accacggtca attttcacga aaagacgcaa ggttttatca atatggatgg
    49441 gaaaaagtac ttatttgcag ctatctggct atttctcctg tatcaagcga ttgagttgcc
    49501 tgcaccgact cagattgcag caagctttac tatttataca cttttttacg tcatggccta
    49561 ccatacggtt agtcattatc cgcgttacga actggatttc aaagacatca aaagaaatct
    49621 ggtagctacg cgagagcttg ttttattttt gatattcttt ggcttattgt ttcttttgag
    49681 aaatgcccga ttgttattcg atgaaaggct ttttgatctt gctatttatg gtttcttgct
    49741 gctgttcatc ttgttcgtgt ttgttttagg acaccagaag aaggaatgga agttgccaag
    49801 ctggctaaat ctgtttactt ttctaaatgg aatgctaggg aatttccttt ttttatttag
    49861 cagtttttat gttggcatac tgtacggatt agaaaaactt tcgattttta tgtatttgcc
    49921 ttatgcagca ggcttgattg cagcaaaaat agtcggacca tggattttga aacgtttaac
    49981 atggtctcca ttggctatgc aattgcttgg actgagttct tctttattgg tcctcttgat
    50041 tccaaatggt tttgcgatag gtgtctttct gcttagtttc tggcacgtag taacaggcag
    50101 ctggctaaat aaggaatatt atcaaactga tacagctatt ccaatggatc gccggatcgt
    50161 tgtcaagtat actactcaaa ataaagggag tgtgatccac cagtttttat tgatgacgct
    50221 tttgtttgtt ttagcaaaag aaatgggaga gccaatccat ctattattga tcacgctgaa
    50281 ccatgaatca gtgccgatag aaagtgtaca actgcttatc ttggcaaaat ggatcaatgt
    50341 tggtctttta ctgagcggat tagtaactgt ttatcattta tggaagaagg cgaaaaagaa
    50401 tgaggctatt cattgatgga gatggttctc cagtaaaaga agatgtcata aaattaggag
    50461 aatcctatgg tcttgacgtg gttatcgtga ccagtgtgga ccattatacg accaaagaat
    50521 acgctaatca tgtccaaatc gtctacgtag accgcggagc tgatagtgcg gattataaaa
    50581 tcgtcggtat ggttaagaag aatgactttt tgatcacgca agattatggt ttagcttcac
    50641 ttgtattgcc gaagggtgtg cgagtgttcc atcactctgg aaaagaattt gacgaagcga
    50701 atatcggcac gcttctagaa caacgttatc atagtggaaa gttaagaaaa gctggcataa
    50761 ggacaaaggg tccaaaaccc tttacggaga aagatcatat gtcttttcgt caagcgttaa
    50821 aaaaggcaat tgactaaggt ttaccaagta cagaagatag taggtaacga aggtatttta
    50881 tttatgctcg aatacatcaa aaagggaaca gggaaacagg ataaatgtgt tccatgttcc
    50941 ctttttgatt gttcttatta ctgggtgcag aacagctttt tcacattttt tattctcggt
    51001 gtttcagaag tagcctcttc gaaaaataag ccgaattttc acaaaaattt gagaaacaat
    51061 ttatgtaaaa ccccttctta tttcttgaag ctgattcttt ctgtcacaac ctttttctac
    51121 tttttcctct tcttatacat ttggatcaat gcatcgattg ccaaaaaaat aatgacgaat
    51181 gcggcaatag catacgtaaa tatccctaat aaggaaacga cctctgcgtt ttctacaggt
    51241 gctgcttgga caagcaggga ggaccctacg atcaatgcag ctaaaatcat accgaaaaca
    51301 agccgattga tcatactttc gatcctgctc aataatttgt cttgcttttt caattccaaa
    51361 ttgacttttc cttgtcccga agtccacatt tctaatagat ggtggagctg ttgagggatc
    51421 ttaggcgcag ccttcatccc ttccaataag tctaatccgc tttgtttcaa tgtatcttct
    51481 acatccagat gagtgagaaa ataatgttga gcaaaaggcg aagcgacttc catgagggaa
    51541 acttcaggat cgaggacgcg gatcacccct tctaatgtcc caaatgcttt cagcaaaagc
    51601 gtgatatcac gatcgaactg cagattattt tgatgacaaa tcgtcacgac atgtgtgaat
    51661 acttcttgca agttgatttc atcaatagga gaatcataat actgttcaag aaaacttgtc
    51721 agttcttggt ggaaccgact ttcatcaaac gagccttctt gctgacaaag tcgaaggacc
    51781 gctttttcga ttcgataagc atctttcgta tacagcgcta aaacggcttg tgtgagtttt
    51841 tgcctaagtc ctgcagaaag atgccccatc ataccaaaat cgatgtagtt gatggtatat
    51901 ggcgcaacag ggtcttctgc agaagtggag gctcgaaaag caaaggaacc gaactctttt
    51961 tcatggactg tttcagtttt tcggctagct tgtgtctgtt cttcttttgt tagtacatgg
    52021 aatagcagat tccctggatg aggatcagca tggaaaaaac catcatcaaa cacctgtttc
    52081 ataaagttct caactaaaag ctttgcaact tcttgtttca gctgcttgtt ttgaattcca
    52141 gtgataaaag tttcagtttt gttttcggcg ttcattaaat gattcaagtt tttaccagac
    52201 atttcttcca tcacgatcac ttttttcgaa caaaaagcgt cataaatctt ggggctgcgg
    52261 atctctttcc agccattgtt cttttgatag aattgttcgc cattttgact ttcttttaag
    52321 aagtccattt cattgatcaa ggaacgtttt acttcttgga gtaccccttt tagatcaact
    52381 acgctagttt caggaatgta tttgatcaaa gggatcgctc gttcaaatag ttgaagatca
    52441 agtcggatct cttcggcgat gttagggtgt tggatcttaa cgactaccgc gtcaccgttc
    52501 ttcagcttgg cacgatgagc ctgccccata gaagcagagg caaaaggcga ttttgaaaaa
    52561 tcatcgaata tatctgataa tgaaagagaa agttctgttt cgatcgtctt tttgaccgtt
    52621 gaaaatgtgt cgctaggtac actatcttgt aatgttttga atgtttgagt gaaggcagaa
    52681 gaaagcaaat cttctcgaac agaaagcatc tgtcctatct tgatgaaggt ggggcctagt
    52741 tcttcaaagg ctttttttac ctgttcggga tttttttgct gtacaaagtt tggcactaca
    52801 ttgtaattga tgaacactcg aatgatttct gccaagcggc tgcgtggatt tgtttgcgcc
    52861 atcatcatcc ctccttttct ttatcataac tttttataaa aaacgggaca aataatctag
    52921 ctgacaaaaa ataaatgacg ctcgttcttt tagaagctgg ctcgtttttt tataaataag
    52981 ttattcgttt atttttgttt tgtgctaaaa tatgagagta aatcactgaa cgatttagaa
    53041 tacaggagga caatcttttg aagattactt tactatatgg cggacgcagc gcagagcatg
    53101 aagtgtccat tctttccgca ttttcagttt taaatgccat ttattataat tattaccaag
    53161 ttcaactcgt atttattaca aaagaaggac aatgggtcaa aggtccatta ctaacagaaa
    53221 aacctgccag caaagatgtc ttgcatcttt catgggaccc aagtggacag acagaggaag
    53281 gctttacagg aaaagtgatc aatccgggcg aaatcaaaga agaaggagcc atcgtttttc
    53341 cagttttaca tgggccaaac ggggaagatg gaacgatcca aggcttctta gagacattga
    53401 atatgcctta tgtcggcgca ggcgtattga ccagtgcatg tgccatggat aaaatcatga
    53461 ccaagtatat tttacaagct gctggtgtgc cgcaagttcc ttatgtacca gtacttaaga
    53521 atcaatggaa agaaaatcct aaaaaagtat ttgatcaatg tgaaggttct ttgctttatc
    53581 cgatgtttgt caaaccggcg aatatgggtt ctagtgtcgg cattacaaaa gcagaaaacc
    53641 gagaagagct gcaaaatgct ttagcaacag cctatcagta tgattctcga gcaatcgttg
    53701 aacaaggaat tgaagcgcgc gaaatcgaag ttgctgtatt aggaaatgaa gacgttcgga
    53761 cgactttgcc tggtgaagtc gtaaaagacg tagcattcta tgattatgaa gcaaaatata
    53821 tcaataataa aatcgaaatg cagattccag ccgaagtgcc agaagaagtt tatcaaaaag
    53881 cgcaagagta cgcgaagtta gcttacacga tgttaggtgg aagcggattg agccggtgcg
    53941 atttcttttt gacaaataaa aatgaattat tcctgaatga attaaactct atgccaggat
    54001 ttacggagtt cagtatgtac ccactcttat gggaaaatat gggcttgaaa tacggtgatt
    54061 tgattgaaga actgatccag ttaggaatga atcgatacca tcagcgtcaa tctttttttg
    54121 aaaaaaatga ataaagagaa ataaagaaga ggctggagtg attgcgtaac cgcgttcatt
    54181 ctagcttcta aatacgaata gagaaattta cttggaagtg agagtcatgc gaacccgccg
    54241 tttaggccaa acgctttttt cacaaccttt tttaggagag atctcaatga atctaacaat
    54301 acaagaaatt gcacaagccg tgggcagcga atgctctgca ctgacaaaga ttaccaatgt
    54361 cgaatttgac agccggaaga taaccgaagg aagtttattc gttcctcttg ctggtacgcg
    54421 agatggacat gaatttatcc agcaagcgat cacaaacgga gcatctgctt cattttggag
    54481 cttgcctgtt gaacaagctc caaaaaattt tccagttatt cctgtagcag atcctttgaa
    54541 agccatgcag caattggcaa cctattactt ggaaaaagta ggcgcagaag tcgttgcaat
    54601 tacaggaagc aatggtaaaa ccaccacgaa agatctaacc gcgtctgtct tgtctgaaaa
    54661 atacaaaaca tataagacgc aaggaaatta caacaataat attggtcttc cttacacgat
    54721 tttgcacatg ccccaagata cagaaaagat cgttttggaa atgggcatgg atcacgccaa
    54781 tgaaatcacg gaactttcac ttatggctca accaaaagct gcagcaatca caatgatcgg
    54841 tgaagcacac atagaaaatc ttgggtcgcg agaaggaatc gctaaagcca aaatggaaat
    54901 cactgacgga cttgtatcag acggattact cgttgtgccc gcagatgaac cgttgctgac
    54961 acctttaacg gcaacattag tacaaacagt cacaacattc ggtattggaa atggcgatgt
    55021 tcatgcggaa gtgacagcaa aaggaaaagc acaaaccgat tttgtagtag aaggagaaac
    55081 atttacgatc ccgctgcctg gaagttataa tgtaacgaat gctttgattg cgtatacgat
    55141 cgggcgcttc tttggactgt ccatacctga gatacgccaa gggctcgccc atgtttctat
    55201 cactcaaaac agaaccgaat ggctaacggc aggaaatggt gcagctattt tgagcgatgt
    55261 ctacaatgca aatccaacag ccatgggact ggtattagat acttttaaaa atctgccaac
    55321 aaaaggacgc agaatcgctg tgttagcaga tatgctggaa ttagggaaag agtctttaaa
    55381 tatgcatgcg caaatgagtg aacatatcga tcctgagaaa ttcgccatga ttttccttta
    55441 tggggaacaa atgcatgcac taaaagaaga attgaaccaa aaataccccc aactacctgt
    55501 gttctatttt gaaaaagaaa agaataaatt gattgatgct atcaaacaaa atcttgaagc
    55561 aacagatagt attatgctaa aaggcagcaa cagcatgggc ttatttgaag tggtagaaca
    55621 acttcagcaa atgaaataaa aatctgtaaa cttgcagaaa acgaaaaact atgttagaat
    55681 aacctattgc cgaaagatga agagaaattg gactggtttt gccatagaaa caacttgttg
    55741 aagccagatg tcataataat gataaagctg aacgcgaccc gtttggcttt tctaatgcct
    55801 aaacttcgca aagtgatttt tcggtggtgc cacaggggct aagaatggat aaaaacgtgc
    55861 gtggccactg tcaaaaagaa acaatacact ttgaccgaac aaatcgacgg ggtagcaaaa
    55921 aagctacatc ctcagttaac aaccatatag gaggattcat ttgaaattta aagaactaga
    55981 attatcacca gaattgttga aatcagtcga acgcgctggt tttgaagaag caacgcctat
    56041 ccaagcagaa acgattccac tagcattagc tggaaaagac gttattggac aagcacagac
    56101 aggtacaggg aaaacagctg catttggatt accaatgtta gaaaaaatcg atccagatcg
    56161 tcacgaatta caaggtttag ttatcgcacc aacacgtgaa ttagctatcc aaacacaaga
    56221 agaattatat cgtttagggc gcgacaaaaa agtacgtgta caagccgttt acggcggagc
    56281 agatattggc cgtcagatcc gtggcttgaa agatcgtcca catatcgttg tgggtacacc
    56341 aggacgtatg cttgaccaca ttaaccgcca cacattgaaa cttggaaccg ttcaaacatt
    56401 agttttggat gaagcagatg aaatgttgaa tatgggcttc ttggaagata tcgaaaaaat
    56461 catttcacaa gttcctgatc aaagacaaac attacttttc tcagcaacga tgccgccagc
    56521 tatcaagaat attggcgtta aattcatgaa aaatccacat catgtgaaga tcaaagcgaa
    56581 agaaatgaca gctgacttga tcgatcaata ctatgtccgc gcaaaagaat acgaaaaatt
    56641 cgatatcatg actcgcttgt ttgatgttca aacacctgaa ttaacaatcg tcttcggacg
    56701 tacaaaacgc cgtgtagacg aattggcacg cggattagaa gcacgcggtt accgtgcaga
    56761 aggaatccat ggcgatttgt ctcaacaaaa acgaatgagc gtattgcgtt catttaagag
    56821 cggtcattta gatatccttg tagcgacaga cgttgctgca cgtggattag atatttcagg
    56881 tgtcactcat gtgtacaact atgatatccc tcaagatcca gaaagctacg ttcaccgtat
    56941 cggacgtaca ggccgtgccg gaaaaggcgg tatgtctgtg actttcgtta caccaaatga
    57001 aatggactac ttgcatgtga ttgaaaactt gacgaaaaaa cggatgacga ctttacgtcc
    57061 accaaccgaa aaagaagcat tcaaaggtca attaggtgct gcagttgaac aaatcgaaac
    57121 aaaacttgca gaaaatggtc tagataaata cctgcaaaca gcagataaat tattagaaga
    57181 atattctgca caagatttag ttgcactttt gctaaaaacg acagcaaaag atccagctga
    57241 tgcagtgcca gtgaagatca caccagaacg cccattgcca atgcagaaaa aaggatacaa
    57301 taaaaatggc aaacgtggtg gcggaaacaa ccgtaatcgt cgtgacggcg gtaattatcg
    57361 tggaaacaaa tcaaaaggcg gttatagcaa aaataataac agccaaaaag atggcggtaa
    57421 acgtcacaat gataaaaaac gcggatttgt aatccgttca aataacgatt aaacataaaa
    57481 tgaaagtttc tcttttttaa gagaaacttt catttttgtt atttgtggac ttatttgtgg
    57541 caaaatggtt ataatttggt agaatgagag tgttctaaat ttattgaaaa gagaaaagca
    57601 tatgataaaa ggaattggga tcgatgcagt cgaacttcct aggatcactc ggctgatcga
    57661 agaaaaacca aaattcattg cacgaatctt gacctctgat gaaatgaaac tattccagtc
    57721 cttgcctttt catcgccaag tagaattttt aggcgggcgt tacgcttgca aagaggcttt
    57781 ctctaaagct tggggaactg ggatcggaaa agttaccttt caagatgtcg agattttaaa
    57841 aaatgaaaat ggtcagcctg ttgtgacgcg ttcacctcat gaaggcaatg tatgggtcag
    57901 tattactcat acaaatgaaa cagcttttgc acaaattatt ttggaagact agaaagaagg
    57961 aaaaacgatg gttgtcgctt ggcaccgccc aacgaaagca gtgatacata aaaaagctat
    58021 aactgaaaat gtagcaaatg aagttgcaag acttccgcaa ggaaaagaat tgtttgcagt
    58081 agtaaaagcg aacggatacg gtcatggagc tattgaaaca gctgaggcag cagttgcagg
    58141 tggagcttcg ggcttttgtg tttcaaattt agatgaagga gtcgagttga gagaagctgg
    58201 atttactcag cccatcctta tcttgaatat ggttccttac gacgcactga ctgtagctgt
    58261 tgctcatgat ttatcagtaa ctgcaggtac acgggaatgg ttgcaagcag cagctgctgt
    58321 tttagagaaa agtaagctag agacgcctct ttcgattcat ctaaaagcag acactgggat
    58381 gggtcgtatc ggattttgta cacctgaaga agtaaaagag gcagctgcat tcatcaaaga
    58441 aagtcgtgta ttagaatggg aaggtttatt tacccatttt tctacagcag atcaggcaga
    58501 tgatacatat tggaatctgc aaaaagaacg ctttatagaa gtgctgaaaa aacttcccga
    58561 acttcctcga tatgtacatg tcagcaatag tgcaacggct ttatggcatg atgaaacaat
    58621 cggcaatatg attcgttatg gagtagcaat gtacggactt aatccctctg ggcacgcact
    58681 accggaagtt tatccattgc agccagcact tgagctggtt tctgaattga ttcaagtcaa
    58741 aaaactccca gcaggtgaag gaatcggcta tggagaaaca tatatcactc ctgaagcaga
    58801 gtggatcgga acgataccga tcggttatgc agatggctgg ccacgaaaga tgcagggttt
    58861 ttctctattg gtagaaggga actactgtga aacgattggg cgtgtatgta tggatcaact
    58921 gatgatccga ttgcctcaag aatttcctgt cggaacaaaa gtgacgctta tcggcaaaaa
    58981 tgcagacaaa gaaatcacta tgcaggatat agcagatcaa ttaggtacga tccactatga
    59041 agtggcttgc ggattaggac agcggatacc aagagaatat caagaataag gaggatccta
    59101 tggtaaaaag aggagatatt tattttgctg atctttctcc agtcgtggga tcggagcaag
    59161 gcggaacacg cccagttttg gttattcaaa ataatctagg taaccatttt agcccgacga
    59221 tcattgttgc ggccataacg gcaaaaatgg ctaaacctaa gcttccgact cacattggga
    59281 tcaaagctac tggaacagga atagaaagag actccgttat cctgttggag cagatcagaa
    59341 cgatcgataa atcaagattg aaagaaaagg tctgccattt ggatcgaccc attatggcag
    59401 aagttgaccg agcattgaaa attagtgtgg gtatcgaagc actgactcct gttactaaat
    59461 aaatatagcg aagtaaccat gcagccttta catataaaaa aggaccagct gtgcgtaaaa
    59521 aaagatacgt ctgcttctag taccagcatt tctgctagaa gaagcgaacg tatctttttt
    59581 gatgatccaa ggagtggtcc atgcgccctc ctcctttaca ttacagccag acttggatta
    59641 ccagctggct ttttttactc ctggaatcaa acctttatgt gctaattcac ggaattttat
    59701 acgggacatc ccgaatttac gcatgtatcc gcgagggcgt ccatcgatta aatcacgatt
    59761 tttcaatcgt tctggacgag catctaaagg aagtttacgc aagccttcgt aatctttttg
    59821 cgcttttaat tcctggcgta aagcagcata gcgttcaatc gtttcttgct gtttttttgc
    59881 tttggcaatc tttgattttt tagccatcaa caaattcctc caattatttt ttattagcgt
    59941 aaatcgtaat ctttacgctt ttagataaat aatttaacac gttacgcgaa aaaagacaac
    60001 tattttgttt tgaatcatgc gccaaaacga aaagacattt tttttgtcaa aataaaggca
    60061 taaacgtaaa cttaaagaaa aagggagatg gtgaccatgc gaagatttgc aattaacggt
    60121 tacggagaag cacaagatgt ttttgaagaa atcgatgctc atcccagaga agtcggttct
    60181 ggacatgttc gtgtagaaat aaaagcattc agtgtgaatc cttatgatgt tgccttgcgt
    60241 ttaggagaaa tgaaagagat acgaacattg aaattccctt atgtaccagg taatgatggc
    60301 gctggtattg tgacagagat tggttcagat gtcacaactg ttcatgtagg tgatcgagta
    60361 gctgtccatg cagtcggcgg gacttacgga gaagaagtcg tgttgccatc agcaaaagta
    60421 gcaaaaattc cagataaaat gagttgggaa gaagcagccg gaatggtcac gccaggaatt
    60481 acggcttata atctaatcaa ccatttgacg gaaatccagc cgacagatac tgtaatgata
    60541 ctaggagcat caggtgcggt cgggtcaagt ctgatccagc ttctccatga aaagggaatc
    60601 cgaatcttaa ccagtgcatc cagtaaaaat gaggaaaagg taaaaaaatt aggagcttca
    60661 gcatttgctg cttatgataa aacaaatcca ggtctgcaat ttgctgatca ggcagacctt
    60721 gtaatcgatg cgacgaaagg aagtatcaaa ggagaaaccg gcatccagat catgaaacca
    60781 ggcggcaggt atgttgcatt aaatgacctc ccagatctag atttgcggca aaaaaaggaa
    60841 ggcttttatg aatcgtttgt tccaagaaag gaatatctag atgcagaagc ctttgctggc
    60901 ataatcaaag cctaccaaaa aggagccttt catgttttta tttcaatgaa tctgtctgct
    60961 tcacttaagc atgtcatcca agcacatcag ctagttgaag gacatcctcc tgctgggaaa
    61021 atcatcctgt cattcgaaaa ataagaaata caggaataaa aaacgaaaaa agctgaaaca
    61081 tttttgtagg ggcaaagaca aaaaatgttt tagctttttt gcgtatacgt ataatagata
    61141 aaaactttgg cataaaatta tctttccctt ttatttcatg ataaagtaaa ggctgtaatc
    61201 cttaattatt tctcaatatg taagagatta caatgagaca attgccgtga tatgtccaaa
    61261 aattcgactt ttttgaaaaa agccgattga aaaaagtatg aaatacatgg taaagtaatt
    61321 cagctgaaaa aagaagaagg gggagaacta tgcagtcaat caaaaatacg cttcgattat
    61381 atttgcttga ctggaaacgg attttcaaaa acccgattgc aacacttctg atcatcgcga
    61441 tcatgatcat cccttcgcta tatgcgtggt ttaacatcaa agcattatgg gatccatatg
    61501 gaaatactgg agagctgcca attgctgttt acagtgcaga caagccagca gagtttcaag
    61561 gtaaagaagt ggccattggg gagcaagtca tcgaatcgct tcataaaaat aaacaattag
    61621 gatggcaatt cgttgattca aaagaacaat tagaagacgg agtccgttcc ggaaagtatt
    61681 acgcaggtat ttacttgcca aaggatttct cagaagatct gctgagtttt actagtggtg
    61741 atatcaagaa accaaaaatc gaatatacgg taaatgaaaa aatcaatgcg attgcaccga
    61801 aaatcaccga taaaggagca agttcgatcc aatcgcaaat cacaaatgaa tttatcaaaa
    61861 cagccagttc gactttattg aaagtattca atgagatagg ttacgacatc gatacgaacc
    61921 tagtaagtat caataaagtt aaagatatga ttttatcaac agatgaaaat ttggacacga
    61981 tcgacggcta cacaaaacaa gtcctcgaat tacaaagtca actacctgaa atcaaggaaa
    62041 aactgaataa agcaaatgaa ttcgtggatt acatccctaa agtagatgag atgggtgaaa
    62101 aagtcgttgc attgaatgac aaaatgcctg agttgaaaga tcaagcaaaa atcatccttg
    62161 atttgcaaga aaaaatccca gagatccaaa atgccggtaa gcaactagca gagatcgacg
    62221 gcgattttgc ttcgatccaa gagacaatga accaaggaat cgatgaagca aaacaaggct
    62281 tgacgatcat ccaacaagta caagatatct tgccagatgt gaaaaattta gaaaatcagg
    62341 caagtgatct agcaaaccaa acaaaatcag gtgcctctca gctgaaagat gcgataccaa
    62401 gcatcacaaa cagtgtagat gttacattga agtcaattgg acaagtggca acaacgacag
    62461 gttcgttaat agatactgta agaacagcta ttgaagatgg taagctgact gatgatgaaa
    62521 agaaaaacat cagcaatgta ttgaacggct ttattgacgg aatatctcaa caacaaaatg
    62581 caatcgataa actggtcgag ttcctgacta aattgcaaac aagtgctgga aatacgaaat
    62641 tacagccaat tattgatcag ctaaatcaat taaaaccctt gctaagcaat ctgcaaaacc
    62701 gcctatctgc tttgaacaca gctgttcaaa atgaagacac gaaggctatt gatgaattgc
    62761 tagatcaaat tgatgccgtt gttaataata tcaatggcat cattgctggt atcaatacag
    62821 gtcaaatcag ttccactgtc acttctctac tagaccaagt catcgatacg attgggactg
    62881 cacaaggcgc actgagcaaa gcggaccaga tcgactttga ttcgcttctc agctcaacga
    62941 aagcaactgt aacgaatgca gtggcaattt tagaaaaata tcaaaaagaa atgcctgcaa
    63001 ttggacaaga aatccatgat gccaatacaa tgctgaatgg aaatatggat accatcgtca
    63061 atggcatcaa tgaaggggca gacctataca acaatgaatt accgatcatt cagcaaaaat
    63121 taggattggc cgctgatttt atccaaaatg attggccagc actaaaaaat gaaatcaccg
    63181 gtacattgac aacagtaaat gaaaaaatgc cagaagtcga aacagctgtg aacgctgctg
    63241 cggacttgat caacaatgac tggccaaata tcaaagctgg tatccaaaaa gcagcagacg
    63301 cgatccgaaa aggcgaacaa gaagtcgatc taggcgaagt catcaaatta ttgaaactag
    63361 acgcaacaaa agaaagtgac ttctttacac aacctgttga gctacaaacc aatgcgcttt
    63421 atccaatcgc aaataacggt tcagctagca ccccgtttta tactgcacta tgtctgtggg
    63481 tcggcgctgt tcttctttca agtgttgcta cgacagaata ctatttagga aagaaagacc
    63541 gaaacaattt tacgaaacga gaacaattcg ttgctcgtat gctgacattt ttgacaatgg
    63601 cgattgcgca aagcttgatc gttacattag ggaatatttt cttactagga gtagatgtcc
    63661 aaaatccggt ctacagcgtg tcatttgctg tcttgatcgc cttagcgttc atgatgatcg
    63721 tctatgtctt ggctgcatta ttcgataatg tcggtaaagg gatcgctatt atcatcttag
    63781 tactgtcgat ctctggcgga ggcgggaact atccgatcca agtctcaggg aaattcttcc
    63841 aaatgatcaa tccgttcttg ccatttactc atgcggtaaa cttgttacgg gaatctgcag
    63901 gaggaattta ttggccaaat gctacgaaag caatctggat catgatcggt ttgtttgttg
    63961 tctttggaat cgtaggaacg gcaatctatc cgttcatcga agaaaaaatg acgaaattca
    64021 aagaagcggc gcatgagagc catatttttc attaaaaaaa gaggttgtga aatcagtgtt
    64081 ttgacctgag tattagagaa aaaaatggaa aaatagttcc tcatattttg ccgttttttt
    64141 gagctaatac cgagggtcag ctgattgaac accgtgtacc aagaggttgt aaaaaagctg
    64201 ctctgcatca agtaataaga acgaccaaat aaaaacagct tcttatgttt ttaatttggt
    64261 tgggcttatt accgcagatg caagctttta aacaccgttt atcaagaggt tgtgacaact
    64321 gcgctatgct tcgagtagta agaatgatgg gtagaaagca gccttacatc tctcttccaa
    64381 aagttaagat atcaaaaagg atagaacttg attttctaag tcaagttcta tccttttatt
    64441 atagaattca aagaaaaagc ccataaaaaa atgttagatt tttgctgtat taaagcgttt
    64501 gaaaaagcgc ttgatatttt tctgtttttt tccaattatt ttcttaaagt ttattgactt
    64561 ttaaggtcag tgagcgtact ataacatctt gtttactata aattttcgac tataaaactt
    64621 gaagtaaaat tgtgtgtaaa ggagtttaga aattggatta cttaaaaagg ttgttagtag
    64681 gtaagccttt aaaatctgct gaaaatgatg agcacaaatt aacccgattt gctgctttgg
    64741 cattattgtc gtctgatgcg ttatcatcta ttgcatatgg tacggaacaa atcgttgtcg
    64801 ttttagtcgc tttgtctgct gcagccatct ggtattcgct acccattgcc gcttttgtta
    64861 ttattttatt gatttcatta acgctatcat atcgtcaaat catccatgct tatcctcatg
    64921 gcgggggagc atatgtcgtc agcagtgaga atcttggaaa gaatgctggc ttgatttccg
    64981 gtggctcttt attgatcgac tacatgttga cagtagcagt atctgtctca gcaggtgctg
    65041 aagcgatcac atcagcggtg ccagcactct acggtcatca agtcgctatt tctgtgacaa
    65101 tcgttttgct actgatgatg ctgaatcttc gtgggctgag ggaatctgca tcgttcctat
    65161 tatttccggt gtacacattt atccttgtga tctcgttact gatcgttgtt ggtttatata
    65221 atatcgtgac aggtgctgtg ccattgcaag ctactgcact tccaggagcg gctgtgccgg
    65281 gtgtttctat cgctttgatt ttacgagcat tttcttctgg ttcttcctct ttaactgggg
    65341 tggaggcgat tagtaatgct gtaccattct ttaagaagcc aagagcaaaa aatgccgctg
    65401 ctacattgac gatgatggca ttgatcttag gtttcttctt cgtggggatc acatttatca
    65461 actattggta tgggatcgtt cctgaaaaag aagtcactgt tttgtctcaa attggtaaag
    65521 ctgtctttgg acatggtatt ttgtattacg tccttcaatt tgctacagcg ctgattttag
    65581 cagttgctgc taacaccggt ttttctgctt ttccagttct tgcatacaat ctggcaaaag
    65641 ataaattcat gcctcacatg tatcaagatc gtggggatcg actaggctat tccaatggga
    65701 ttatcacact agcgctaggt tcgatcgtat tgctgtttat tttccatggc tcaacagaac
    65761 gcttgattcc gttatactct atcggcgtgt tcattccatt tgctttatct caaacaggga
    65821 tggtcgtcaa atggaaaaaa gaaggaaaaa gatggttaag caaatcgatt gccaatatta
    65881 cgggtgcgtt catctcatat gcaatcattg cgattttgtt tgtctatcgg ttaggggaca
    65941 tttggccttt cttcatcatt atgccaatcg ttatgttcat tttttataag atccatgatc
    66001 attataaaaa agtagcagaa cagcttcgtt tagaaaatga tgcaaaacta catgattatg
    66061 acgggaatac cgttcttgta ttagtcggga atgtgacacg ggtaaatatc ggtgcgttga
    66121 attacgcacg ttcgatcggt gattatgtcg ttgcgatgca tgtttcgcta gacgaagatg
    66181 tcgaaaaaga aaaagaaatc gaagcagaat ttaaaaagca tttcccagat gtccgttttt
    66241 caatcgtcca ctcttcttat cgctcgattg aaaatccaat tatccgttat gtagatatcg
    66301 taagtaaaaa tgcagcgaaa caaaactata caactactgt gttgattcct caatttgtac
    66361 caaacagacg ctggcagaat attctccata accaaacaag tttgcggtta agattgcgat
    66421 tgtcttggcg tgaaaatatc gttgtcagca cctatagcta ccatttgaag aaataaacca
    66481 agttttaaaa tcaaaaacag accctctagc tcttaattga agctagaggg tctgttttgt
    66541 cttattctca gctaaaggtg ccctctataa gccattaaag atgggaacag cgtatgaaat
    66601 gttattattc ttcctattta gctgtaaaac attttgattt tgatgtcttg atcataattt
    66661 ccgaatcctt tgccaaataa tgtacagcct cctatatttt cagcttcttc ctttacctcg
    66721 actcttaagt gccatgtgtc ttcataagta ggaatctcag cctcgtcaag tgaccatttt
    66781 ttgttaccat aactcagtgc catttttaat tggtgattca caaacccaac ggtctaagca
    66841 agtatcaaat cgatagtgaa catgaatgac aggagacgaa agaggcaatc atttttgagg
    66901 cagtagaaga cttgacggcc aaaatcagtt ttgaataacc gttacaaatc tttcgtgcgt
    66961 atcctcaggt tattcaagat atcaatcaag tcacactgca acgaggaacc ttcgtgtatt
    67021 ggtgttgttg catagactca caggctttgg attcgacctt tgctaaagat gatacgttgt
    67081 taccaattac tttacgtttg gctaccgcta gtcgtaaagg agtaagaagt gagcgtggac
    67141 aaagatcctc cctaaaaagg ggtctttttc tctatgtaaa aaagaggaaa agtttgctga
    67201 atttaagtgc tttattgtat ttttttgacg tacttaatca agtacgctat aattatatca
    67261 gaaaaaggag gagagacaat ggctaataca acgatgaatc ctagcactgc tcgtaaaaat
    67321 ttttatcagt tattgaaaga ggtaaatgaa aatcatactg aaatcgagat tattagtgat
    67381 cgtagtggaa acaatgctgt attgattggg ctggaagact ggagagcgat tcaagaaacc
    67441 ctttttcttg agcaaacagg tactttagat aaagtacgag accgcgaaaa agacgacagt
    67501 agctttacga atatcgatga tattgattgg gaagctcttt gatgagtaac tatacagtcg
    67561 caatcaaaaa ctctgcgaaa gtggatttaa gaaaaatcaa gcaaacgaat ttaaagaaac
    67621 aatttgaaga agtgattcag acattgaagg aagatccata tatgcccacg cagtcttttg
    67681 aaaagctgag acctacacat gaaggcagat actcacgcag gctgaataga cagcatcgag
    67741 tggtttacaa agttgatgag gaaaataaag ttgtagaaat ttattctgcc tggacacatt
    67801 atgagtgaag tgagaagagc caaccttttt agatagttaa caaagagtct ccccaaaaag
    67861 gagactcttt ttttgttcta aaaccaacct tcttggattt attgcatgat ttctttaatt
    67921 tgttcctata tctccacctt tgaagggtca ttgtttgagt ttaggaatgg aatgttgacg
    67981 ggtttatgta aaacaaggag gaagaagtgt gacaaggaaa atcagtaaaa ttggagcatc
    68041 gttgttagtg ttggcagtgg caacgaccat cgattatcgc caaagtgagg cgaagacaat
    68101 tggaaatgtc gtttatcgag aaacagccaa aagtatgttg aaaaaagcaa tggacaatca
    68161 accggagtcg tcttattggt tcccagagga ttcgttggca tggtcttttg gaaaagatac
    68221 agatacgaaa tacaatacca gtgtcgttcc tttagcagag cgggtatcca aagcgacatt
    68281 gccgaacatg aatgtcactc aaacggaaga agttaaagtc gtggcgcttt cggccatgaa
    68341 tagcagtatc aacggcaatg catcacgagg gatcgatatc ttttacgcca atgtcttttc
    68401 ttattggcaa tatatcgatc aattggttta cgggggtgct cttctggtga aggatcatcg
    68461 tatcgccaag tctagttgcg acggatgcag tccataagaa tcgtgtaccg gttttaggaa
    68521 ccgcaaacct cccatggcga gaagctggaa tggctagata tgtttttagc caaggatgag
    68581 gatggcaagt tcccgattgt ataaaagatg atcgaagtta ccgatacggc agtcaccagt
    68641 tttgatgatg cagctgaagg catcgaacaa tcgaggactg atggaaaagg cttaaatgaa
    68701 agccacgcca aaggatgtaa gaattgatgc ccaattcaaa gaacaagcag acgagtattt
    68761 agagatcatg tggtatgaat tccatgacga gtgatgggaa aatggattgg caaaatgcct
    68821 tagcggataa gaaaaaaact tagtcggtgg atgcagatat gaacccgttg gcagtatgaa
    68881 gttggagtat acggaatgga taaatagcta agaaaagcaa ctctttgccc ataaggaggg
    68941 aagaatcaga gtggattatt gccttaaaaa tgacagaact aaaaaagcct ttgcgaccaa
    69001 caaatggtcg cagaagcttt tgttttttac aataacatca gcgtgatgta cggctggctg
    69061 gatctagccg tctttccaaa atacctaaaa gccagtccgt gatgatcgcc attaatgcag
    69121 ttggcaatgc acctgctaaa ataatcgatg tgccctctgt tgcattggtt cctcggataa
    69181 tgatatctcc aagaccacca gcaccgacga atgcaccgat cgcggttatc ccgatggcaa
    69241 caactaacgc gtttcttatg cctgccatga taacagaaac agagagtggt aactctacca
    69301 tgaatagtcg ttgtctagca gtcatcccca tgcctttgcc gacatcaagg atgtttttgt
    69361 ctacttggat catcccggta tacgtattct tgatgatcgg taagagggaa tacaaaaata
    69421 cagtaacaat cacgacattg actcccaggc ctaatccaat gattaaaatg gagatcatgg
    69481 ctagagaagg gatcgtctgg atgatattcg ccaaacgaat cacccagttt gctaatttac
    69541 gtctcctaga aatcatgatc ccaaccggaa tcccgacaac tgctgcaaag aggacaccat
    69601 agatagaaat caggaagtga cggataaatt gtgcaaagat ataggagcca ttttcctgga
    69661 aataatagaa aaattgttga aagacattca ttttatccaa ttgcttcact tcccttcaaa
    69721 atagtggttt tcttgtaaga aacgttgtgc tactactgat ggttccacta gattattatc
    69781 tgcttcgtag ttcaattcct gcattttttc cgtactgatg gtatgaccta acttatttaa
    69841 ggcttctttt aaatgaggcg tatcagctaa tatcttgttg tctacaactg gagcagcatc
    69901 atatggtggg aagaattgac gatcatcttc caacatgact agatcataac tggcgatccg
    69961 tccatccgta gagtagccaa gaacaatatc catttttcct gcttcgactg cgtcataaac
    70021 aagtccaatc tgcataggta atatcgaatt gaaggagaag ccataagttt cttggaagcc
    70081 gtcataaccg tctcctttac gattgatcca agaagtatct acaccagcta ctaattcatc
    70141 cgctacattt ttcagatcgc tgactttaga tagatggtat ttttgtgcgg tgtcttttcg
    70201 aacaagaaag acatacgtat tttcaaaact ataggacggg aaccacgttt gaccgtatct
    70261 tttctcaaat tcatctttca cggtagcaaa cgcttttttc ggatcttttt caggaggaag
    70321 gttcaatgta gtcgttaaat ctgttcctgt gtagcgagcc gctgaaattg aagcatctcc
    70381 gttcatcatc gcttgatggt tgatagttgt agtagccaga ttgttgatga ttgcggtatt
    70441 tttatctgta taatgttcaa ccattccggc aactagactt gctaagatct gtgcttctga
    70501 agtgattcca cctgtgatcg aaatggtgtc ttcatctgta ttgctggcaa gaccagggaa
    70561 ggaacaactc gcaagtataa gaagagagaa aagaaccatg gtcattttta gtatacgttt
    70621 cattcttcat tctcctctct aagtgcgatc ggtgtcagcc ggtgttccaa gagacctaga
    70681 agccaatcag ccaatagtgc taaaattgtt acagggatcg tacctgccaa aatgagatct
    70741 ggttggtaat tattcagtcc actaaaaatc aaatcgccta aaccaccagc gccgatataa
    70801 gaggcaagtg tagcccatgc aatcacgtaa acggcagcta aacgaatacc agccatgatc
    70861 gttggcatag caatgggcaa ttcaacggaa aaaatggatt ggaactcagt catccccatg
    70921 ccttttgcca catcccgata attccaatcg acatttttca tcccaatgta cgtattacgt
    70981 aaaatcggca ataatgaata aataaagagt gcgataatcg caggtaattt acctacgcca
    71041 aatagcggga tcatcaatgc caataaagca agagaaggga ttgtctgcaa ggcactggtt
    71101 aagccaatca caatcgtggc agcttttgga aaacgtgtca gtaagacacc aagcggtaca
    71161 gcaatgatga taccaatgat caaagcaagt cctgaaatat aaagatgttc aaaacctttt
    71221 gagagaattt ctgaaccgtg ctgatgaaaa aactgctgca taggtcagac ctccttagat
    71281 tcttcaggag tagctgactc tgcaacatca gtagttgcgg gatcttctcc ccaaatcaca
    71341 tcgtagacga tatccactaa tgatgcacga gtcaggatac ctacgacttt gttctgttca
    71401 tcaacgaccg gcacgtattt cagcccgcgt tttaaaatgc gttgcaaggt atcacgaaga
    71461 taggcggatt cttttacgta aaaaacatgt ttattcaaaa tgtcgccgac actggatact
    71521 tttcctcgtt ttttattcaa cgtttctaca tcgataaatc cttttaatac attcgaatta
    71581 tcggtaacta acaacgtatc tacacgtttt tccctcatca gtttgattgc ttcttgtaat
    71641 gatttttccg gtgtaatcgt gattgctgta tttagcataa cttcgccgac tcttgtcgca
    71701 tttggcttgg cttgtagtag gcgatcttct ccaatcaact cttctacgaa atcgtttgca
    71761 ggatgacgta aaatattttc tggtgtatca aattgatcac acggccttca ctcatgatgg
    71821 cgatacggct tgctagttta atcgcttcat ccatgtcatg ggttacaaat acgatcgttt
    71881 ttcccaaacg ctcttgtaaa tctttgacaa ggtcttgcaa ggaatcacga gtgattggat
    71941 caagcgcacc gaatggctcg tccatcaaga tgatatcttg attcgctgca agggcacgaa
    72001 cgactccaat acgctgctgc tgaccaccag ataattcatg tgggtagcga tctaacatct
    72061 cacggggcaa ttccacaaga tcaatcattt tttcagctgt tttattccgt tcttcttgac
    72121 tgactttcaa caatttttgg acaagagtga tattttcacg gatcgtcatg tgaggcatca
    72181 atccaatact ttggataaca tagccgatct gacgtcgcaa ttccactgga tcaatttctt
    72241 ggatggattt tccatcaatc aaaattttcc cttgtgttgg atcagtcatt cggttgatca
    72301 tccgcatcgt cgtcgttttt ccgcttccgc tcgtaccgat aaaacaaatg aactcaccct
    72361 tttcaaatga aagattgacg tcctctacag cgacttttcc acctttataa atttttgaca
    72421 cattttgaaa ttcaatcatg gtgccacctc tttttctatt ttcaatgaag aactatctat
    72481 tactagtatg cctaaaacaa tgaaaaaata caaatagggt agttttttta tttaggaaaa
    72541 caaaaaatag aagtgaaaaa gtgctgattt tgccaacaga acgaaaaaca tgatagaatg
    72601 atgaagcaca tgatagaaag tgagtgacga tatgttaaca acagaagatt ttgattttga
    72661 cttgccggaa gaattaatcg cacaaacacc attaaaagac cgcgatcatt ctcgtttact
    72721 tgttttggat cggaaaactg gagagatgga agataaacat ttccatgaaa tcattgaaga
    72781 gttacagcct ggtgatgcgc tagttatgaa taatacacga gttctgcctg ctcgtttata
    72841 tggagaaaaa ccagatactg gtgcgcattt agaagtcctt cttctaacga atacacaagg
    72901 agatacgtgg gagacattga tcaaacctgc gaaacgtgcc aaagttggga caagaatctc
    72961 ttttggtgat ggcagattgc aagcagtagt gaaagaagaa ttggaacacg gcggacggat
    73021 cattgacttt gagtatgaag gaatttttct agagattctt gaatcattgg gagaaatgcc
    73081 attgcctcca tatatcaaag aacgtctgga agacccagat cgctatcaaa ctgtctatgc
    73141 gaaagaaaat ggttctgctg cagcgccaac agctggattg cattttacga aagaattact
    73201 tgatgagatc gaagcaaagg gtgttgaatt agtctatctg acactacatg tagggttagg
    73261 tacattccga cctgtcagcg tagataaaat tgaagaacac catatgcata gtgaattcta
    73321 ccgtttgacg gaagaagcgg ctgagcgatt gaaccaagta cgtaaaaatg gcgggaaaat
    73381 cgtggctgtc ggaacaactt caattcgaac attagaaacg attggaacga aatttaatgg
    73441 agaaatcaaa gcagacagcg gatggactga tattttcatt acgcctggct atgaattcaa
    73501 agtggtagaa gctttctcaa ctaatttcca tttgccaaaa tcgactttag tcatgttagt
    73561 tagtgccttt gcaggaagag aattgacttt atctgcttac cagcatgcga ttgaagagcg
    73621 ctatcgtttc tttagttttg gagatgcgat gttcgtgaaa taaggttatg aaaagccgct
    73681 agttttgaat aacaaaaaag ctttacaaag attttatgac aaagaaaaag ccgagctctc
    73741 gataagctcg gctttcttgt acctcggaag gattcaaata aacagaaatg attgtaactt
    73801 tccatcatac atgcaaagca cggtgttgaa tttcttcatg ttaaacaaaa attttgtttt
    73861 cgttacatct tcaacatttg aaacatctct tgttcctatt caattttcag acgtttacgt
    73921 tattcaattg ttacgtatcg cattacttct tgtttcggat acttcgtgat ccaaacatcc
    73981 atacgcattc actatttata ggtttgtact ctacacgcat gtctgggtga aacctgtact
    74041 tctaacaact ttgtttgctt acactgttct atcgagaaat tcttctgtct atttgatggt
    74101 atcctccttc cttacatcta tataatagca tcctttggta gcgtttgcaa atataagcac
    74161 tttaaatttt acgctttctt aacaaataat cgataagccc tttatttgaa gtaaataaaa
    74221 aaaggatgtt atagtaaaat aagtaccttt ttttgtacga agattttaaa ggaggaatag
    74281 gatggaagaa tacaagcaga caatagaaga agtaaagaag gtaacagaag cagatccaga
    74341 tactgggttg tcttcacaag aagtggatga acggcgaaaa aagcagggat tgaataaatt
    74401 cgatgaggcg ccgaaagaat cgatgatcaa aaaattcttt cgaagcttgt ccgattttac
    74461 aacgatcatc cttcttgtag cagcggtgat ttccttttat accgcatttg ctacagaaca
    74521 tggggattta tttgaaggac tgctgatcat cgctatcgtt gtgatcaact cggtattggc
    74581 gattgtccaa gaaggaaacg cagaaaaagc actggaatcc ttgcaagata tgaataaaca
    74641 aacggcaact gtcatacgag acggcaaagt gaccactgtt gagtcagagc aactggtagt
    74701 gggtgatatt cttgttttag agtccggtga tgctatttct gccgatgccc gtttgatcga
    74761 agcatcacaa ttacgtgtag aagaatcggc gttgactgga gaaagtgaag cagttgaaaa
    74821 agatgcagca tttattgctg aagaagatga gtcattaggt gaccaactta atatggtatt
    74881 taaaggatgt acggttgctg caggtcgtgg gaaagcgatc gtcactgcca ttggcatggc
    74941 tacagaaatg ggtaagatcg cggatctttt gaacgaaaat actatgcaaa aaacaccttt
    75001 acaggtccga ttgaatcagc taggcaaacg aatcagtatg atcgctttag cggctgcggc
    75061 tttagtattt gtgattggtg aactgcaagg agaaccactc cttgagatgt tcatgacttc
    75121 gatttccttg gctgttgcag ctgtaccgga aaccttgact gttattgtta cgttgacact
    75181 tgcatacggt gtgcagaaga tggcgaggaa acatgcgatc atccgacagt tgccagcagt
    75241 tgaaacgcta ggaaccgcta atgttatctg ctcagacaaa acaggaacgc tgacacaaaa
    75301 tgaaatgcgc gtccgccggg tatggagcaa agaagatgaa gtaacgaata tcgaagattc
    75361 aatgacgaac agcgcgatgg aaatattgaa aatggctgca ctatgtacag atgtgacggt
    75421 cgagcaagaa gatgacgacc ttgttatcaa aggtaatcca acagaagtag ccatcgtaag
    75481 ggctgttgaa gagaattatc atacaaaggc agaattagaa gaaaaatatc cacgagtaaa
    75541 cgagcttcct tttgattctg aacgaaaaat gatgacaaca gttcaccaaa tgggcgaaaa
    75601 atacatctca atcaccaaag gagcattcga tgttcttgct ccgcgcttta gttcaggcga
    75661 tgtagaacaa gcggcgatcg ttaatgatcg ctttggtaaa cgggcactga gagtgattgc
    75721 tgttggttat gctacttatg acgaagaacc gcaagatatt tcttctgaag cactggaaaa
    75781 agacttacgt ttgattggtc tgatcggaat gatcgatcca ccacgtccag aaagtaaagg
    75841 agcaatcaag cgagcgaaaa aggctggcat caaaacagtc atgatcactg gagatcatgt
    75901 ggtgactgcc agtgccatag cgaaagaatt aggtatcttg aatgatccgt cagaagcact
    75961 ttcaggatca gaactgcatc aattatcaga cgaagaatta gatgcgcgtg tcaaagctct
    76021 ctctgtctat gctcgggtaa cgccagaaga caagatccgg atcgtcaaat cttggcaacg
    76081 tacgggtgca gtcgtagcga tgactgggga tggtgtgaat gatgcaccag cgttgaaagc
    76141 aagtaacgtt gggtgtgcca tggggatcac aggaacagat gtggcgcaaa gtgccgcaga
    76201 tatgattttg acggatgaca actttgcgac gatcgtagac gctgtcagtc aaggacgttc
    76261 ggtttatcag aatattcgta aagctatcaa ctttttgcta agctgtaata tttcggagat
    76321 ctttattgtt ttgattgcga tgctgttagg atggggagca ccatttacag ctgtgcaact
    76381 actgtttgtc aatgtagttg ctgatggact tccaggattt gctttaggaa gagagccagc
    76441 ggaacacggc atcatggatc agccacccat tccgaaaaat gaagggatct ttgctcgagg
    76501 tttattgcaa aaaattgcca taaatgcagg ggtcttcacc atcgtaacct tgtttggtta
    76561 ttatttaggt agttatgtcg atactatcag cccatgggta gacgcaagtc agcatgtcgg
    76621 tcaaactgtt gcattcttga tcttagctta ttcatcgatt ctgcatgtgt tcaatgtacg
    76681 cagcagccaa tcgatcttca aagtcaattt agcgaccaat aaagctttgg tagaaatggc
    76741 gttactggca ttagctatta caacggctgt tgctttgctg ccatttacgc aagaactatt
    76801 cggcctagta cacatcagtt tgaatcactg gttcttggta gggattttat ctatcgtacc
    76861 aatcgcggtg aatgaactga tcaagttcca tcgattaccg gaagcagaag aagaggaatg
    76921 attaactgtt tctaagttga ttttcttttt actggaacta tacaaaaagg aggtgtgaca
    76981 tgaatgttgt cacacctcct ttttgctaat gggctgaatg tttcttttac tgcctctcga
    77041 taaacggtgt tctcctgcca ccccctcggt cacaagtcac actctctttt aaatatcttt
    77101 tggcaattgt tcataaaatg ctgcttttgt tgcagaaatg tatgggctga gccatagata
    77161 accaatacca gcagttgcta atgctaagat atgccagccg ataaatgaaa gatctaacca
    77221 gaaaagttgt cctttataac catccatcaa ttttcgactt gcagtgatcg tatccagtac
    77281 tttaggtgtt tgtcctgttt cttggacgat atcgtagtaa ataaaatagc tttgagaata
    77341 agaatagctt ttaatgattc caggaataac taatagcaaa ccccataatg ttgtaaagat
    77401 cgagatcagc acagctaagc tgatcactcc gccgataaac aaacctttga atccacgaaa
    77461 tgcatctttg aatggttcga tctgatcttt ttgtccacgt aaaatatcaa gataagtcca
    77521 agaaataccg ctcatgaaca aagcgctaat gatgctggaa ataattccgc caccgcctcc
    77581 tgaactaccg cctcctgaag ttgtcgcttc tgagacgctc gctgatccat catatatggt
    77641 ggtggcaaac aagataatag gaatcataat aagaagaata gtagcaatac caatcaatgt
    77701 agggattaga tttagtaaaa cagcttgtcc ccaacgtcct tttagactct cttttgcttg
    77761 gcgttttaat tctgcagaag attgcatgat ttttccctcc ttttctttaa taacaccaat
    77821 ataccatgta tcaaaaaata tttctgtcat aatgtttttc ttttttttgt tattcataaa
    77881 aaaaggataa aaaaaagaat attaaataaa gtagaaggaa gccgagggaa cttgcaaaaa
    77941 gcacttgaaa cgattatgat ggtgggagtt gtcaagatat tttatataga aagtagggaa
    78001 atgaatgacg caacctgcca ttcgctatcg tttaatcaag aaagaaaaac atactggcgc
    78061 gcgtttaggg gaattgatca cgccacacgg aactttcccc acaccaatgt tcatgccagt
    78121 aggaacatta gctacagtaa aaacaatgtc cccagaagag ttgaaagaaa tgggtgctgg
    78181 cgttatttta agtaatacat atcatttatg gctacgccct ggtgaagact tagtagaaga
    78241 agctggcgga ctgcataaat ttatgaactg ggatcaaccg attttaacag attcaggcgg
    78301 ctttcaagtt ttttcattaa gtgatatgcg gaatatcgaa gaagaaggcg tgcatttcaa
    78361 aaatcacttg aacggttcgc gtatgttttt atcaccagaa aaagcaatca acatccaaaa
    78421 taaattgggc tctgatatca tgatgagttt tgacgaatgt ccaccattcg atgaaagtta
    78481 cgactacgtg aaaaaatcag tagaacgtac gtctcgatgg gcagaacgag gattgaaagc
    78541 tcatgctaat ccagaccgtc aaggattatt tggaatcatc caaggagcag ggtttgaaga
    78601 tcttcgtcgt caaagcgcga aagatctagt cgggatggat ttccctggtt actcaatcgg
    78661 cgggttatct gtcggggaac ctaaatcaga aatgaatcgt gtattagaat tcactacacc
    78721 gttgatcccc gaaaacaaac cacgttactt gatgggtgtc ggtgctgctg attcattgat
    78781 cgatggtgtt atccgtggag tagacatgtt tgactgtgtc ttgcctactc gaatcgctcg
    78841 aaatggcaca tgtatgactt ctcaaggccg tctagtagtg aaaaatgcga aatatgccca
    78901 tgatttccgt ccaattgatg agaaatgtga ttgttataca tgtaaaaact atacacgtgc
    78961 ctatatccgc catttgatca aatgtgatga aacatttggg attcgtttga cttcttacca
    79021 caatttatac ttcttgttga atctaatgaa acaagtacgt caagcaatca tggatgataa
    79081 tttgcttgaa tttagagaag ccttttttga agaatacggg ttcaataaag aaaacgcaaa
    79141 aagcttctaa aggaaaatct tttaaaaatt aagtaaaaaa ctagtgttat cagcattttt
    79201 ctgctaaagt gttttttgta gatacagaat tagtgaggtg aaaaaaatgg gaacattacc
    79261 aatgcttgtg atgtttatcg cgctattagc aatgtggttt tttatgtcac gttcgcaaaa
    79321 gaaacaacag caagaacgtc aaaacttgtt agatagcatg aaaccaggtg attcagtcgt
    79381 aacgatcggc ggattacatg gagttgtttc agaaatcgac aatgacaaac ggacagttgt
    79441 tttagactgc gaagggatct tccttgagta cgatcgtgca gcaatcaaaa cggtcaaacc
    79501 agggactgtc gtaacaaatg atgcaacgac tactgttgaa acaacagaag aaccagctga
    79561 gacagttgaa gtaaaaactg aaacaacaga aacgctggaa gagacagaag agaaggaaga
    79621 aaaataagta tcaaagctta tttaaagacg aggtagggca gaaagatgtc ctacctcatt
    79681 ttttattcaa aaaagaaaga gataagagca tgggatatat tgctcaatat tgaataatcg
    79741 ggtgtgtcat ataaataata ataaataact ttatatattt ttctatacaa acacctgtaa
    79801 ataaagcaat tttatattta aaaataactg tgaaaaaaat cacaaaaaag tgtttacata
    79861 gaaaaataaa tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta
    79921 ggaggataat gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag
    79981 caatgattga tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg
    80041 atcaagaaaa agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata
    80101 tgccattagc gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa
    80161 tcaaaaatat gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg
    80221 gtgtgatcaa tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg
    80281 tttgcggagt aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg
    80341 cgttgaaaac aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg
    80401 cagaagcagc acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta
    80461 tccaatggat cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta
    80521 tcgcgatcgt cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa
    80581 aaccagcttt aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa
    80641 ttaaacgtgc agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg
    80701 cttctgaaca agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc
    80761 aagcacacca agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga
    80821 tgaacgaagg aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg
    80881 caaaattggc aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag
    80941 gcgtaggccc agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca
    81001 aagctaacaa cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat
    81061 taggacatac agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc
    81121 gaatgaaagc ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata
    81181 tctataacga aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg
    81241 tttctcgtaa cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa
    81301 ataacatgca atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt
    81361 acttagagaa aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc
    81421 aattcggtta ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga
    81481 tcgaagtctt ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga
    81541 aactgatggc agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg
    81601 acgcagctaa gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa
    81661 aacaaaaatt cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac
    81721 aattcgtatg tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca
    81781 tcactgacag tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg
    81841 tcgcaatcgt cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg
    81901 ggatggatgt gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata
    81961 cacgtggatt gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa
    82021 aaacacctga tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg
    82081 cgtttgctaa cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat
    82141 acgggatccc tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg
    82201 caaaagatcc acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag
    82261 attacgcaga tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg
    82321 aagctttagc aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa
    82381 aagctcaagg agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag
    82441 cttacgaaga ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac
    82501 agatcatcat tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa
    82561 atagatgaaa aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt
    82621 aactgaaagt tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa
    82681 aagcgatatt tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt
    82741 aaaaaggtgg cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga
    82801 aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat
    82861 ttttgttgga ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc
    82921 cacaaggtgt cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg
    82981 cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa
    83041 acgtcaatac aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt
    83101 cacgctattt gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga
    83161 caaatgaaga cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg
    83221 acgatacatt gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc
    83281 cagaatattt agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata
    83341 aacgaacagg attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag
    83401 cattttgggc tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct
    83461 tagaattatc gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag
    83521 tgcgtgcatt aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc
    83581 cttcttctta cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg
    83641 tccataaacg ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat
    83701 tgcttgaaca aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg
    83761 gggacagttt agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt
    83821 taacggtgct ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat
    83881 atgaaaaaaa catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta
    83941 agcggctgcg gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata
    84001 gttaccttgc gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa
    84061 tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat
    84121 ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc
    84181 acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt
    84241 ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg
    84301 gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct
    84361 ggtcagcgca gcttttatat ggtagacggt ccgatccata cgccggaaga tttgaaggga
    84421 aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt
    84481 tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac
    84541 ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac
    84601 tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa
    84661 gaacagctaa ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt
    84721 tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa
    84781 catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt
    84841 catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa
    84901 ggagcgtagc tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt
    84961 tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa
    85021 atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt
    85081 cttccgcgta tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat
    85141 taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg
    85201 ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct
    85261 cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac
    85321 tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc
    85381 aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt
    85441 ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat
    85501 cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa
    85561 aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg
    85621 gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg
    85681 tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt
    85741 ccatctctag ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc
    85801 aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga
    85861 caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg
    85921 cttcgatctc tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa
    85981 tcatgctagt agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac
    86041 agatggctca tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca
    86101 tcattatcgg tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg
    86161 tcatttactc cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac
    86221 ctaatatgtt gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt
    86281 cttccatgat gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga
    86341 ttatgggcgt aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac
    86401 tggtgggtat gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac
    86461 caatcgtcac aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc
    86521 tgtgtgtagg ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag
    86581 gaggcgtaaa agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag
    86641 tcgtcttgtt tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga
    86701 gttaggagaa tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa
    86761 acgatagaag atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc
    86821 ttaggtcttt cgtttcctga aataccttct ggcgcaaatg agatcaattc tgggatggga
    86881 aattatgtca aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat
    86941 atcaatatga ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag
    87001 tatcttcgct atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt
    87061 ttgccggaaa tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg
    87121 tccgttatcc gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa
    87181 ccaggtttga atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta
    87241 gattcggact atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat
    87301 caagaacaag tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg
    87361 gttcatttga aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac
    87421 ggtgtgatcg agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat
    87481 gtggttcttg aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa
    87541 tgtaaagaag acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc
    87601 acgtctcttc ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg
    87661 gtataataaa attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa
    87721 tcagcggtga acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag
    87781 atgtcgtcag ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta
    87841 cattaaaggg agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa
    87901 gtgtgctgct catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat
    87961 tcgagcgtgt ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt
    88021 ctaatctatc ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag
    88081 aacaggaagt attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt
    88141 acggtgaaga cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag
    88201 ttgtacaaaa agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg
    88261 aacaaatgtt acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg
    88321 aaaagcagtt ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat
    88381 ttacgtttca tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag
    88441 aataccgcag ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc
    88501 tgaaagcatt caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac
    88561 agaaacagtt ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa
    88621 ataaccgcag tagctgctgt tgtttacata gaagcagaaa ctgcggttat tttattttta
    88681 tctatatatt tcaaaatatt ttgaaaaaat gacgtacata ttatatgaat cagctgaatt
    88741 tacgaaagtc tctcggcgat agtcctgttt ttgctttgaa caggcggcta aagtaagcaa
    88801 aattctcaat ccctatttct tcggaaatca ttcgaaccgt aaattctgta taccgaagaa
    88861 ggtctttagc gtaagaaagt tttttgctga tcaaataatc gattggtgga gaaccgataa
    88921 atttggaaaa atctttgctc agctgatatt tattcaataa gaattgttgt tctaattgat
    88981 caagtgtaat cgtttctttg aaatgttcgt ctaagtattg cttcagtttg taaacgtagg
    89041 cgggaatatc ctcttcttcg aaatctagct gatacttttg caaaatcagt tcattcagca
    89101 actcgtggat agctacagag gactggaaat cggtacgggc attcgtgcct tgttgcatgt
    89161 ggatcaattc tgtgatcaaa tggtggatct tgttttctga tggatctcct gttgaatgga
    89221 aggtgttatg gccgtctttt gtgaactcct gataaaatga ttggacgttc tgaccattca
    89281 aatggatcca atccatttcc catggttctt cgctgatcgt ttgataacgc tgatattctt
    89341 ggcagtcaat aaaaaataag tctccagctt ttagctgttg ctgattatca ttatatgtaa
    89401 gcgaaccttt gccagacaaa gtaaacttta tcaagtaaga aggtaaatgt gctcgttcag
    89461 tatagtaagg gattggtgct ttgaagtaac cgatttcttg gatataaaaa aataaattcc
    89521 tgcttctttg tgtaggcgtc aagatcactc tttttgagtc ttccgaccat gagtggttgg
    89581 ctttttccca gtaatccatg taaacactcc caacttgtca agataatgca attatatacc
    89641 aaaatagtgt gaagcaaaat ataagattct gtattatgat agcattgtca ataaatgata
    89701 acgctttatt aataaagtgc taatcaacaa taaagtaaaa gaggtggaga aatgaatcca
    89761 acatttgttg ccatcgatat cggtgcatca agcggacgac taatgaaaag tacgagggat
    89821 gcaaaaggtt ctttgatctt ggaagaagta caccggttca aaaacggctt tcggaacaaa
    89881 gacggtcacg atcgctggaa gatcgattat cttatcaaag agattttgat tggatttgaa
    89941 aaattaaagc agacagggat tgatcgcctt tttgcgggga tcgatacatg ggcagtcgat
    90001 tactgtctga ttgatgaaca aggggatcgg ataaatgatc cgattgctta tcgggacaag
    90061 cgtaccaatg gagcagtaga acagttttct gaaatctatt ctctagataa gttgtatgag
    90121 cagacaggga ttcagatcca gcctttcaat accttgttcc aattgtttgc tgaagaaaaa
    90181 gaaaatttgg caaaagcaca taaattgtta ctgatacctg attatttagg gtatgtcttt
    90241 actggtaaca tggtgacgga gaaaactaat gcatctacta tgcagctgct gaatgcaggg
    90301 acaaaagaat gggaaataga acttcttgaa aaaatcggtg tctcgaagga attatttcca
    90361 ccattgacag agccgggaac gatactagga gatctgcaaa aagaacaatt ttcaaaatat
    90421 gatttgccag atgtgacctt tatcacagta gcaagtcatg atacggcgtc agctgtatta
    90481 ggaacgccag gtcaaggaga acgttggggt tatatcagca gtgggacatg gtcgctttta
    90541 gggattgaaa caaagatcgc caatgtttct cgcaatgctt tttctgaaaa ttatacgaat
    90601 gaatggggtg cccataacac ggtgcgtttt ttgaaaaaca tcatggggat gtggttgatc
    90661 caggaagttg cccgaatgca agagtacgag tacagttatg cggaattggc agagcttgct
    90721 gcaaaagaaa aaccgctgca gcaagtgatg gaaatcaatg accctcgatt tttgaatccc
    90781 ccaaatatga tagaagagat tcagcaatac tgttttgaaa cagagcagcc agtccctgtc
    90841 tcatcgggag ctatcgctcg ttgtgtgtat gataatcttg ctctttgtta cgcacaagag
    90901 ctgatcaaag tagaacactt aactggaacg aaattggaaa agctgcacat cgtagggggc
    90961 ggatcgaata atcgtctact gaatcaattg acagcagatt atgccggaat cattgtagaa
    91021 gcaggaccta gtgaagccac agcaatcggg aatgttgcca tgcaaatgct cagtacaggt
    91081 cagttttcga atatagaaca agcaagaaat gtaatcaaac agtcgtttcc atgtgaaact
    91141 ttttacccag atgaaaataa gcaaaacacg atggaatggt acgtgaataa aatcaaagga
    91201 gtaatcgtat gaacacgatt gaaagaaatt acgaacaagc aaaagaaaaa tatgcaacta
    91261 tcggtgtaga tacagatgct gtactagaaa agatacagga catcaaaatt tctatgcatt
    91321 gctggcaagg agatgatgtc aaaggtttct tgacaccgga tggcgaattg accggtggaa
    91381 tcatggcgac agggaatttt ccaggagccg cacgaacacc tgaagagctt agacaagatc
    91441 tggagaaggc ttattcgttg ataccgggca aacacaaatt gaaccttcat gctatctatt
    91501 tggatacaga agaatctgtc gatctaaatg aaattgagcc gaaacatttt gaaaaatggg
    91561 tagagtgggc aaaaaaagaa gaaatcggtc ttgacttcaa tccgactttc ttttcgcatc
    91621 caatgatgaa agacggcttt acattggctc atccagataa agaagttcgt gaatactgga
    91681 tcgaacacgg gaaaagaagc cgcaaaatcg cggagtactt tgggaaagaa actgggcaaa
    91741 caagtatcaa taacttttgg gtacctgatg ggatgaaaga taatccgatt gatcgtttta
    91801 ccccacggga acgtttaatg gaatcactcg atgaaatttt tacagaagag ttagatgaac
    91861 ggtatgtaca agatgcagta gaaagcaaac tatttggttt aggcgctgaa gcttatacag
    91921 tcggttccca tgaattttat atgggttacg ggttgactcg tgacaaactt gtttgtctag
    91981 atgctggaca ttttcatcca acagaagtga tttccaataa attatcttca ttagctttat
    92041 tcagcaaagg aatcatgttg catgtttccc gtccagtgcg ttgggattca gaccatgtgg
    92101 ttttgatgga tgatgaattg caggaaatcg ctaaagaatt agtccgtaac gaattgttgg
    92161 aaaaaacaaa tatcggtctg gatttctttg atgcaacgat caaccgtatc gctgcttggg
    92221 tcatcggtac gagaaataca caaaaagcac tgctgaaagc tatgctggaa ccagttgagc
    92281 gattgaaaga aatggagctg gcttttgact ttacttcaag aatggcttat acggaagaat
    92341 tgaaagattt cccgtatgcc gatgtatgga attatttctg ttacaaaaat caagttccag
    92401 ttggtttgga ttggctggaa gaagtccagg aatatcaaaa agacgtgttg gaattaagaa
    92461 aataggagtg tggctatatt gaaaaaaata gatattttac aagcgcctta tgtgaaagaa
    92521 atgatcgaaa cgacgtcgaa tctttatcgc ttgggatggg atgaaagaaa tggcgggaat
    92581 atttcttatt tactaaaaga agaagagatc acaccgttct tggatcctga aaaagtgatc
    92641 cgccgcatct cgatgatttt tgatgccagc cgtttagcag gatgctattt tattgtaact
    92701 ggttcaggaa gatattttaa aaatgtagcg agcgaacctg ccgagaatct tggaatcgtt
    92761 cgagttgctg aagatggcaa aacactggaa cttttgtggg ggctggaaaa cgaatgcctt
    92821 cctacaagcg agttgccaag tcatttcatg agtcatatcg cccgtttaga agtagaccca
    92881 gataatcgga tcgttatgca ttgccatgct agtcatctat tagcgatgag ttttacgcat
    92941 gaattagacg aacgttcctt tagtcgtacg ctatggcaaa tgtgtacgga atgcttagtt
    93001 gtttttccag aaggaatttc catcattccg tggatggttc cgggaacaaa tgagattggg
    93061 gaagcaacag ctgaaaaaat gaaagaaact cgattggttt tatggccaca acatggaatc
    93121 tacgggtcag gaaaagacat ggatgaagtc tttggtttga tcgagacagc agaaaaggca
    93181 gcagaagtct atacgtacgt caaagcgcaa ggaccaatct tgcaaacaat cacggacgaa
    93241 aacctgtggc atttagcaga tgcgtttggt gtcactccaa aagcaggtta tttggaggaa
    93301 gttcatacga aagcaggtgt ctgacatggt gaaaaaagct ttttgcatga aagtttaccc
    93361 agaaaaacaa gaagaataca aaaaaagaca tgatgaactt tggccagaaa tgcgtacagc
    93421 acttcgtgaa cacggggtca tcgactattc gatctatctt gatccgagta caagtttgtt
    93481 atttggttac ttagtgatcg aagatgaaaa gttatgggaa aaaatggcaa atacatctat
    93541 caatcaaaaa tggtggaagt acatggaaga tatcatggag acaaatccag atgcctctcc
    93601 agtcagttgg gaattgatgc cagtctttga actgtagcaa caagcaactg ggcaaccggt
    93661 tgcttggggt tctttcgttt ttattgtgaa aaataataca aattaggagg aatagaaaat
    93721 gagtaatcga atgattttaa atgaaacatc gtattttgga aaaggagcga ttgagtcgat
    93781 cgtccctgaa tttcagcagc gaggattcgg caaagcagct gtcattactg acaaaggatt
    93841 gatcgagcac ggtatagcaa caaaagtaac agaacttcta gataaagcag caatcgctta
    93901 tgctttatac gacgggatcg tacctaatcc aacgatccaa aatgtaaaag atggagtggc
    93961 ttttgttaaa gaggcagagg cagactgttt gatcgcaatc ggtggaggtt cgccaatcga
    94021 tacggcaaaa gccattggga ttattttgac gaatccagag ttttcagacg tcattagttt
    94081 agaaggtgtt gctgatacga aaaatccatg tttacctata ttagctatac caacgacttc
    94141 tggaacagca gcagaagtaa cgatcaacta cgtcatcaca gatgaagtaa atcaccgaaa
    94201 atttgtttgt gttgatcctc atgatatccc aatcgtcgcg ttcatcgaca gcgatatgat
    94261 gatgggaatg ccgaaaaaat tggctgcatc aacaggtatg gatgctatga cgcatgctat
    94321 tgaagggttt atcacaaaag gcgcttggga aatgactgat atgctgcatt taaaagcaat
    94381 cgaaatcatc ggccattctt tggaagcttc tgtagatggc gatcaaaatg ggcgagaaaa
    94441 aatggcatta ggacagtata ttgcgggaat gggcttctca aatgtcggtt taggactggt
    94501 acacgggatg gctcacccgt taagtgcatg gtacaacatt cctcatggcg tagcgtgtgc
    94561 agcacttttg ccaactgtca tgaaatataa caaagaatat actggagaaa agtaccgtga
    94621 gatcgctctt gttctaggga ttaaaggagc agctgaaatg tctttagaag atgtaagaga
    94681 agcggcttgt ggtgagatcg atcggttgag caaagcggtt ggaatcccag aaaccatttc
    94741 tgaattaggt gtaaaagaag cagatattcc agcaattgca gaagatgctt tgcgagatgt
    94801 atgcacacca ggaaatccaa gagaaacgac agtagaagaa atcattgctt tatatcaaag
    94861 tttgatgtaa aaacgaagcg gatcttcaac attaaattgc ctgtaaagag aaatggtagt
    94921 ttatatctgg atatctcttt taaattgatg gtatttccgc tataattttt cttataaaga
    94981 aataggagaa tattatatgg aaaataaaaa gccttatgga actgtattga tcaaagcctc
    95041 taaaatacta gactatttag cggaaaacca agatgtctct ttgcagacga tcgcaaaggg
    95101 tgtgggaatg acctcttcta cagtcttgaa aatactagat actttgttga tgatcggcta
    95161 cgtaaataaa aattcagaga aaaactatcg tttaggtgca aagcttattc gctacgcgaa
    95221 taaaaacatt gaacaaatcg atttggtaga gttgacttta ccgtttttag aacagctgca
    95281 gcaaaagatc gatgagacta tccacttagg ggtattggac aacaatgaga tcttgtacgt
    95341 gaacaaacta gatccaaaac atcaaacgat ccgcatgtct tcaaaaatcg gaatcacccg
    95401 accattatac aattctgcga tgggcaaagc cgtattagca gaattcagtg aagagcagta
    95461 cactgattat cttgaaaagc atccgttgat cccttacaca gaatatacga tcaccaatgc
    95521 attacggttg aaaaaggaaa tcaaaacggt aaaagaaacc cacatcgcat ttgacgatga
    95581 agaaatcgaa cgtgatattt tttgtatagg agcctcaatc gtgaaaaacg aacaaatcat
    95641 tggtgcattt agtgtaagta tgccgaaata tcgtttaaca gatgagacaa aagaacaaat
    95701 aatcagtgcc ctcaagcaaa caaaaagcga aatcgagcaa agaattcaaa aataatgaaa
    95761 aaagcgtttg caatttttct tttctgccgt ataatatggg tatagatttc caagttataa
    95821 aataaaattt ctaaatagga aataggtgga gaagatgaaa aaaattgata ttttatctcg
    95881 attgaaaaac gccggagtga ttgcggtggt acgcggaaaa agcaaagaag aagctttgaa
    95941 tgcttgccac gcaatcatca aaggtggtct aactggtatt gagttgactt ttacagtccc
    96001 tcaagcagat caagtaatca aagaactttt gtcattctac aaagaccaac cagaaatagt
    96061 gattggcgca ggcacagtat tagacgctgt cacagctcgc ttggctatct tagctggcgc
    96121 agagtatatt gttagccctt cctttgatca agaaacggca gagatgtgca atctgtatca
    96181 aattccttac ttaccaggat gtatgactat taccgagatc aaaacagcgc tgaaaagcgg
    96241 ggtagatatc gtcaaactat ttcctggaag cgcttatgga ccaagtatca tttctgcctt
    96301 caaggcgccg atgcctcaag tcaatatcat gccaactggc ggtgtgagtc tggacaatat
    96361 gaaagagtgg ttcgatgctg gtgtcgtaac agtcggagta ggtgggaacc ttttagcacc
    96421 cgcagctaca ggagattttg acaaagtaac ggaagtcgcg caacagtatg cagcaaaaat
    96481 gaaagaaatc aagaggtagc tgcttatggg aaaagtagtg acactcggag aaatcatgtt
    96541 gcgattgtcc acagagcagg gaacccgtat cgattcaagt gaacgattcg ctgcccatta
    96601 tggcggaggg gaagcaaacg tagcaatttc tttagctaat tacggacatg aagcgatttt
    96661 tgctacaaaa gtacctgata acgcattggg aaaagcagtc aaaaagcact tgcaatgtta
    96721 tggagtagat acgaaacagg tattatctgg tggatcaaga ctaggtactt attatatgga
    96781 aactggtagt ggcgaacgcg ccgcctcagt gatctatgat cgtgccggat caagttttgc
    96841 tgaaatggat catttggaat ggtcgctaga ggagttattc caaggaatag atatatttca
    96901 tgtttcagga atcaccccag ctttgtctaa aaaatggcaa aaaatgacga aacaggtgat
    96961 agaagctgca aaaaaagctg gctgcctgat cagcttcgat atcaactatc gtggaaaatt
    97021 atggacacag caagaagcca gcgaagtaat ccatcagctt ttacctttag tagatatctg
    97081 ctcagctgga aaaatggatg ctctttattt gttaggtatc gagaaagcgc cagaagcaga
    97141 agaacaacct ctaatttatt attatcaaaa aatgcaggaa aagttcccga atattcaggt
    97201 gttttattcg accaagcgta ccgttcattc agcaagtgaa aatgaattga aggggacatt
    97261 gtggatgaac catgaatatg tggaatccca agtccataga atcaccccaa tcgtggatcg
    97321 agtaggtggc ggagatgctt ttgcaggtgg cgtgctccat gggattttat ccaatatgtc
    97381 tccgcaagaa atcattgatt ttgctacggc agcttctgcc ttgaagcata cgatccatgg
    97441 ggactgcaac caatttagtc aagaagaagt cgcacaattt ttagcatgcg gctctggaaa
    97501 aatcattcga tagaaggagt agaagaaaat gcaagaaatg gaaacacgtt atacacacag
    97561 tccagaagat atccgtcatt attcaacgga acaattgaga aaagaatttt tagtagaaaa
    97621 ggtatttgaa ccagggaaaa tcagtttgac ttatacgcac aacgatcgaa tgatctttgg
    97681 tggagtgaca cctactgaaa aagaattaga aatcacatta gacaaagaac taggtgttga
    97741 ttacttctta gagcgtcgtg aactaggtgt gatcaatatc ggtggaccag gattcatcga
    97801 gatcgatggg caaaaagaag aaatgaaaaa acaagacgga tactatatcg gaaaagaaac
    97861 acgacatgtt gtattttctt ctcaagatgc tgaaaaccca gctaaattct atattagttc
    97921 tgttccagct catcataaat atccaaatgt aaaaatcagt atcgaccaag taaaaccaat
    97981 ggagactggt gaaggcctga cactgaatga acgtaaaatc taccaataca tccatccaaa
    98041 tgtatgcgaa agctgtcaat tacaaatggg ttatacgatt ttagaaccag gaagcgcttg
    98101 gaatacgatg ccttgtcata cacatgaacg tcgtatggaa gcttatgtct actttgatta
    98161 tgccaatgaa gatacacgtg tgttccacat gatgggcaaa ccagatgaaa caaaacattt
    98221 agttgtagac aatgaacaag ccattatttc gccaagctgg tcgatccatt caggtgtagg
    98281 tacaagcaat tactcattca tctgggcaat gtgcggagaa aatatcacct atacagacat
    98341 ggatatggtg caaatggatc aattaaaata agcataaggg agagaaaatg atggaattta
    98401 gtatggatat gtttcgttta gatggaaaag tagcattagt caccggtgct gtctatggta
    98461 ttggatttga gattgcacgc tctttagcta cagcaggagc aaaaatcgta tttaataact
    98521 tgaatcaaga atcagttgat gaaggtatag cgcattataa agaagcaggc atcgatgcaa
    98581 aaggttatgt ctgcgatgtg accgatgaag aagcggttca agcgatggtt caacagatca
    98641 aagaagatgt aggttcagtt gatattctag tcaacaatgc agggatcatc aaacggacac
    98701 cgatgatcga aatggatgcg gcagacttcc gccaagtaat cgacgtggat ttaaatgccc
    98761 cattcatcgt ttcaaaagca gttattccgg atatgatcga aaaaggcggc ggtaaaatca
    98821 tcaatatctg ctccatgatg agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg
    98881 caaaaggcgg attaaaaatg ctgacgaaaa acatcgcttc tgaatacggt caatacaata
    98941 tccaatgtaa tggaattgga ccaggttata ttgctacacc gcaaactgct ccattacgcg
    99001 aaacacaaga aaacggtgaa cgtcatccat tcgatcaatt tatcgttgga cgtacgccag
    99061 cagcacgctg gggcgagcca atcgatttgg ctggaccatc tgtcttcttg gcttctagcg
    99121 catctgattt cgttaacggc catgtcttgt atgtggacgg aggcatactt gcctatatcg
    99181 gaaaacaacc ttaaaagagc tttagtacac tgtttagtgg agaggttgtg aaatcagcgg
    99241 attgaacgaa taccgaagga cagctgatta aacatcattt attcgaaaaa agaggctggg
    99301 acagaagcat ttaactccaa gaaataagaa ggaattcacg aaaattgctt ttcaaatttt
    99361 tgtgaatttc agcttatttc cgaaggagtt gcttttactt ccgccgtttg tacgtgttta
    99421 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct cttttctgtg cgtcttccat
    99481 acttcactaa gtcgatccct cttgcttcgc accaatcctt cactggtccg gaaaagatca
    99541 gagattgttg gtgtagggcc gcggtattcg ttgcgccgac catagtataa agcagacgaa
    99601 gctcttcttg ccattgcttc atcagtataa tagtttcttc tacgccgtga ttcatcagat
    99661 gcgtcagtac ggttcctgaa gtacccacgg catttgcgcc gagacaaagg gctttaaata
    99721 tgtcataggc attgcggata cctccggagg ctaggatttc catagaagtg tctgcttcat
    99781 ttgcttcaag caatgaagag actgtcgatt gcccccagtc agctaaataa ctcagttccc
    99841 gcttggagcg acgggcgttt tcgatctggg tgaagcttgt tccgcttcga ccgctgatat
    99901 caactgtatg aactcctaaa gcagctagat cattgagcgt ttctcttgtc atgccaaaac
    99961 cgacttcttt tacgatgagg gggacgtcaa tggctgtttg agtttcttga ataagtactt
   100021 tccaattggt aaaatcacgg tctccctcag gcataaccaa ctcttgaggt gcattcaaat
   100081 gaatctgtaa ggcatctgcg tgaaacaatc ggatagcttc tttggctctt tcgacagaag
   100141 tccctgcacc gatgttggca atgattttcc cgtgaggata ttcttgtcgc ataatcgtat
   100201 aagtatcggc taaagaaggg actttcaagg cagcgctgac tgaaccagtg gcaatcatca
   100261 aatctgcttc tcttgcgacg attgcaagat cttggttgat tttttttgtt ttttcgcttc
   100321 cgccagtcat cgcattgata taaaatgggc tgcttagtgt aaacccaact gcctgtgtac
   100381 ttagatcgac atcagaaact gctatttgcg gcaaagggtt atggatgatc cgtacgaagt
   100441 caaattcgtt tttttgttta tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc
   100501 gattcatcgg ttttcctttc tttgatcgct atagacatgt aacggcagtg gagtgatttc
   100561 tgctttttcc catgcactca ttaaaggaag aatgccagat ttctggtcaa caattacgat
   100621 tccgcaatcg cccccacctg caccagaaga ttttgcggct ccttcatact gttcagctaa
   100681 attacacaat ttgttcaaag caggcgtttc gatgacgacc ccagtgattg cagataaatc
   100741 atgcagtaat tgtcggtttt ttcgaatcat ctgttggatc aacgttacat tattttcttt
   100801 aaaccctttg atcatttcgt tgacacattc tgtactattt tttaagaaaa gctgataagc
   100861 caccatttta tcttctctcg aacggtgaac ttgatcgacc aaatcagaag tagaggcagg
   100921 gctacccgtc caaccaatca ataaacgtaa atcttcagga gcaatcaatg gctcaatgga
   100981 tagacctggc caatccaggg caagtaactc actgatagaa tgctgagttt cttgttcttg
   101041 gagccaagga tggtcgaagg ttgagaaagc gatccagcca ccataacagc tagcagcgat
   101101 gtcgccgcag gaaccattat cttgaactgc taaattggct agtgctgcaa tcttgaaaat
   101161 ctccaactga gataaattca acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt
   101221 cacggcaccg ctagaaccca atccatattt ccggccattt gaactgtcta actcacttgt
   101281 tactttcaga tcataaaagg ataaaagaat gtttttttct tgtgcatact tttcagtcaa
   101341 gcgaatagca gcaaggatat aatgaaaagg attttcccga atatctaaaa ctaattctcc
   101401 gttgcgtctt gtccaacgta caggcatccc actatattgt gcagattgga tacttccgac
   101461 ttttcgtgca gattctacag tgactgtcac gaattgatcg actgcagcga taactgctgg
   101521 atggcctgtt tcaacaactg catattctcc ggcaatatag agtttgcctg gtgcagatac
   101581 ttcaatcata aaaatctccc tttattcaat aatcgcaatt cctggaccag catgtgcagt
   101641 aattacttgt tctttgctaa ataatttggt aaatgtttct tgtacctcag ataaatggct
   101701 gttttccact aaaactttga cattagggcc agcatccatc gtaaagtagc atggaatgcc
   101761 ttgctttctc aattgtcgca ccgcatccat cgcttttaag ctgtcaggag accagtaagt
   101821 gaatggaggc tgggcagcta aagtcgttcc atgcattttt agtccgtttc tttccatcgt
   101881 ttctccgaga agttggaaat cttttgtttt gattgcttgt ttcaattgat atagatcccc
   101941 ttcaacggaa tctaaccagc cttgataaaa actggatgtt tcgactgtcc gacgcatccc
   102001 atttctgctg gacacttctt ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc
   102061 gtcttcgaaa gaatcggaag ggactggctt agcgtaagaa cttaagtcgt catggccttt
   102121 ttcccattcg acgaaaccac cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc
   102181 aaatcttgaa agagagagat catctaatcc aagcttaaga gcagtattgc aagcgcctgc
   102241 taaagcagct agcccgctgg cagaggaggc taatccagct gcggtaggaa cgaaattctg
   102301 actgattact gaagctttta gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg
   102361 actgactttt tttgttgctt tttcgtcttg caaggtgcca tctagataaa attcatctac
   102421 catatagcta tcagaaaaga tgacttctgt ctctgtataa aatgcatcca atgttaacga
   102481 aaggctgttg ttcattggaa ggatgagttc ttcatttttc ttaccccaat atttgattag
   102541 agcaatgttg gtatatgcgc gtgctttgcc tttaaacata ggtatgtact cctaatcctt
   102601 gtatccaagt ttcagcagca cccgcatctt caagtgcttg actgatttct tgggcttttg
   102661 tttttgtttg tgctaatgca atcatacagc caccgcgtcc accgccagtt aatttggcac
   102721 ccaatgcccc ggtatctttt gctgtttgga tcaatagatt aagaaaatca ttgctgattg
   102781 tcagcttttc cagaagtgat tgtgattcat ccattgtctg tgccaacgtt tctggtgaat
   102841 tttcaatgat tgcttgtttt gcttgcttcg tcaagtatcc taatttttgg atcatttgtc
   102901 cagtttcatg aggctgggtt tcaaagaggt gagcgacatc tttgacggct tctcttgttt
   102961 gtcctttgat tcctgtatca gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt
   103021 cgaaaggatg cccttttgta aaataaatcg gctgcaagct gctagtagcg gctgcatcga
   103081 ttccactagg attaccatgg gcgatttttt ccgaaagctg gacattttct aatagtattt
   103141 cacgagacaa aggaaatgct aagtaatcat aaaaagcacg agtgactgct gtggcgactg
   103201 cagcgcttga tcccattcca cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt
   103261 ttttttggat gtcatgctgt ttttttaagt gaatgaataa ttttttgata ttttttaatg
   103321 catgaggagc ttcggctaaa tttcctgaat agtaggaaga aaccagttga tcgtccattg
   103381 catccagctc ttcaaggaat gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg
   103441 gttctccata aacaaccgcg tgctcgccca tcaatatgat ctttccgctt gattctcctt
   103501 ggccatagtt tgccataatc taactccttc tcacgtaaaa gtgattttta agcaaaaaat
   103561 aaagcgccca aaacgagttg cgctgcttca cgaatttttt tgtccactaa gggtctcaaa
   103621 aaacattcct cctttatttt actaaaacca tctcagaagt aaaactaaga atacgttttc
   103681 taaagaaaaa acactttttt cttaagaatt ttttgacttt ttttcttctt tgactagaaa
   103741 aaaatacaat tgacactact agatattggg ggtatattta aaaaggttgg agttaaacac
   103801 aatatttagt gatgaagtga gctggaggta aatgagcgtg atcgttttag caggtacaat
   103861 tggtgccgga aaatcaagtt taacagaaat gatggctgaa cactttgata gtcaggcatt
   103921 ttatgaatcg attgatgata atgaagtctt gcccttattt tatgcaaacc ctgaacagta
   103981 tgcttttttg ctgcaaattt attttttgaa taaacgtttt gcaagcatca aacaagcaat
   104041 gaaagatgac aataatgtat tggaccgttc gatttatgaa gattcactgc ttttccatct
   104101 aaatgcagat ctaggaagag caacagaaac agaagttcgt gtgtatgatg aactgctgga
   104161 aaacatgatg gaagaacttc cctatgctgc ccataaaaaa catccggatt tattagttca
   104221 tattcgtgta tcttttgata cgatgttgga gcgtatcgaa aaacgtggtc gttcgtatga
   104281 acaattgtcc tttgatccaa gtctatacga ttattacaaa gaattgaaca gacgttacga
   104341 tcaatggtat gaagaataca aagaaagtcc aaaaatccaa atcgatggag accgctataa
   104401 ttttgtggaa gatcctcaag caaaagaaga agtcttgaaa atgatcgaag aaaaattagc
   104461 agagattcgc caaaccaaag tagccatttg atttacagtt gctcaaataa agggtaaacc
   104521 tttatttgag cgactttttt ctttatactg gaaacaaatg tcaggaggat agagtatgcg
   104581 ggaaaaacac tattttaatc aagaccgtaa tttttggatc aatgtacagc aaaacgatgt
   104641 gctagctgtt aaaaacttaa aagaagaata tggcattagt gacgagatgc tgacttattc
   104701 tctagataaa aatgaacggg cgcgcgtaga gtacgattct tttgatgaag cgttactgct
   104761 tgtatcaaat gttccgcacc agcaaaaagt cgataatcat tatgaaacta gtcctatcgc
   104821 ttttatttta aaagaagacg gtttgtttac atttaccacg ccgaataccg agtacgtgat
   104881 tcgactaatt cgatcgcttt tggaacgaat gccagacatg agtgtataca gtttattgtt
   104941 tcgtacattg tttttgattt ctgattcctt ttttccttta atcgaggaag tgaatagtga
   105001 acgtcagcgg cttaatctga aattaagaga aaaaacaacg aacaagaatc tgctgcaact
   105061 ttcagattta gagatcggtt tggtttactt agttacagga acaaaacaaa atgtagtact
   105121 tttagaacaa atcaaggcat tggctattta tcgcaagttg tcagaaaagg aaaaagaaga
   105181 gttggacgat gcgctgatcg aagcacgaca agcagttgaa atgacgaact tagcttcgca
   105241 gattttagac cagctctctg gcacttataa taatttgctg aataacaact tgaatgacac
   105301 gatgaaattt ttgacggtct ggtcgctgat cttgaccgtt ccaactattg tgacaggatt
   105361 cttcggaatg aatctccagt taccctttac ccattccgtt ttcggatggg gaatcgcctt
   105421 gatcatcagt ttggtcttat ctatttggat gcttattgca ctgtggcggc ggattcgttg
   105481 atctagtctt tgatctgcaa accaagggct tgggcgatca caagtgcttg ctcttggttg
   105541 acgattagtc ccgccaaaga atcttgagat aaagcgattg ctgtgaagaa attagtcgta
   105601 aagtctaatc ccttcagaga agttcgaaac cagttcgaac cggtcaactg attgtcagac
   105661 aaaaaaaggt ttttccaagt gacctcataa aattcgctgt cttctaattg acaatgatcg
   105721 aaggagacag ctttcaagtt tgtactacta aaggaagcca tcgaagcaat acaatcagtg
   105781 aaagtacagt ctcgaagata actttcggca aaattcgtac ctgttaattt acattgatga
   105841 aacacgactt ggtggaaact gccaccaatc cactccagat tggaaaaatc acatttttca
   105901 aaaatgacat tactgcattc aaaatgacgt aaaactgttt tttgcatcgt aagtttttgg
   105961 atatgactct ctcttaatac catatttttg gcatcgagat agctttggtc acccccgttg
   106021 atcagaagac cagagaagat ctcctcatct tctaaataaa actctccctt tgtaagaaga
   106081 ggaagagaag gtggaatagg ttcgattttt ttcatgtggg ttcacctcgt ttttctttag
   106141 tatagcaaag aagcgggaat cactgcaaga aagtctgcta atctaatcat tttcagaaaa
   106201 ggaagacaaa ctgtatgaaa aacattgaca aaaagattag tttttgctat actatgatgg
   106261 ttgagaaata aaagcagaag cacccgcttc tcgccttagt tgataatgtc actgggctag
   106321 atagttttca ctggttctat taggtgaagt acgcgcgggt tctgtaagag aactcgtttt
   106381 tttattgccg tttattcggt aagcttttct ttctctcata atatttggag gtgaatgacc
   106441 atagcaaagg atatgatggt taacgacggc attcgtgcac gagagttacg attgatcggt
   106501 tcagatggtg agcaattagg agttaagaca aaagcagaag cattgaatat tgcagaacaa
   106561 gcaaacttgg atcttgtgtt agttgcccct ggtgcgaaac caccagttgc gcgaatcatg
   106621 gactacggta aataccgttt cgaacaacaa aagaaagacc gcgaagcgcg taaaaaacaa
   106681 aaagtgatca atgtaaaaga agttcgctta agtcctacga tcgacttgaa tgacttcaat
   106741 acaaaacttc gtaatgcacg taagttcttg gaaaagggcg ataaagtgaa agcttctatc
   106801 cgtttcaaag gccgtgccat tacccataaa gaaattggtc aaaaagtctt gaatcgctta
   106861 gctgaagaaa ctgcggatat tgcaacagtg gaacaaaaag cgaaaatgga cggacgcagc
   106921 atgttcttga cgctggcacc gaaaaacgac agtaagtaat ttgaatgact aactggctgt
   106981 tggacagccg atagtcggat aacgaaagat tttgaggagg aaatatagtc atgccaaaac
   107041 aaaaaacaca ccgcggatca gcaaaacgtt tcaaacgtac tggtaaaggc ggattgaaac
   107101 gcttccgcgc gtttacaagt caccgtttcc acggcaaaac aaaaaaacaa cgccgtcaat
   107161 tgcgtaaagc aagcatggtt tctagtggcg acttcaaacg tattcgccaa caactagcaa
   107221 aaatgaaata agaagtacaa ataacagaat ctgaagattt atttttatta ggaggaatta
   107281 tacatggcac gtgttaaagg tggaacagta actcgtaaac gtcgtaaaag agtgctaaaa
   107341 ttagcaaaag gttactacgg ttcaaaacac actttattta aaacagcaaa agaacaagta
   107401 atgaattcat acaactacgc atatcgcgat cgtcgtcaaa agaaacgcga cttccgtaaa
   107461 ttgtggattg ctcgtatcaa cgcagcagct cgtatgaatg gcttgagcta ctcaaaatta
   107521 atgcacggtt tgaaagttgc tgaaatcgac atcaaccgta aaatgttagc tgacttggct
   107581 gttaacgatg cagcagcatt tactgcattg gctgaccaag caaaagaagc tttggctaaa
   107641 taagctaaac aattataaag aggaaagaag ataacgaaaa gttatgttct ttcctctttt
   107701 tttatttaca atcctccatc aaatgaaaca ggacaataac gtataagtct aaaatagcat
   107761 aattaaggtt ttttaattga aaactgaaca tttatggtca ccctttaatg aaagcgctaa
   107821 ctacataagt tagaatgagt ttgactgata tattagtttg cttatcagaa aaacaaaatg
   107881 gacggaggaa taacatgagt gatattatta ttggtatttt gttagttatc tcattctttt
   107941 tcatggtctg gtactgtgta aaaggctaca acttgatggt cggctttgcc atcatggcta
   108001 ctgtatggat gggattagcg ctagtcggaa acacgttttc accaaacccg gcgatggaag
   108061 ggcaaggagt aattgatgtg ttgacgcata tctatacgac ggggccagct gagtacgcca
   108121 aatcgatctt ggtaaatgtc ttctttggtg cctttttcgg gcgtgttcta gtagatagcg
   108181 gaatcgcagc taccctgatt cgaaaggtag ttgaacttgg aggggacaag ccaagaatca
   108241 cgatgagctt actatgtatt gtaaccgctg ttatattcat gtcgatgaca ggaatcggac
   108301 cagtgatttc tattgcagtt attgtgttgc caatcttgat gtcattaggt atttcagtac
   108361 cggttgcact gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca
   108421 atttcaaaca atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt
   108481 ataatgacta tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa
   108541 cggtcgctaa tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag
   108601 ctgaaggtgg cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg
   108661 tcgttctttt agatcttcca atcatcttag gttttattct agcaggtatc tgggcattgc
   108721 tgtttactgg aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat
   108781 ttacagatgg cgcagtagat gttgcgccaa tggttggttt cttgatgacc ttagcgatgt
   108841 tcaataattc agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac
   108901 cgcaatcacc attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc
   108961 gcggtccaat gaatctagtc ggttcaggct cagctatttt agctgtcgta ttagctgtta
   109021 atccaacgat gtcgccagcc tttttgttcc cgttatttgc aatcacaacg attgctcctc
   109081 agcatttaga tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt
   109141 ctcgagaata tatgaagaaa tccatcccaa ctggttggat catcggagcg atcttatgcc
   109201 tgatcacgtt cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag
   109261 atgaatggag gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac
   109321 acatacgaaa gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt
   109381 gaaaacaacc cacgatgatg tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa
   109441 ttgtttgctg gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac
   109501 acaagcgact aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc
   109561 aaaaggtgga ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt
   109621 agggaataag aaaaaaatag agattgtcaa tgcattctta cctgtcaaac acttaaatgt
   109681 agaccgtgtt tcagaaacga tttcttcttt agaaagagaa agagcggagg tcttggtttc
   109741 ttctatggca tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc
   109801 ggtaaaatcg atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag
   109861 aagaactcgg acagcagcaa tcaatgcatc tattttacca aaaatgctag atacggcaac
   109921 ctctacagaa gattccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg
   109981 agacggcgga gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc
   110041 cggtccagca gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta
   110101 ttttgaagta ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat
   110161 cgattattca attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct
   110221 aggtgtggca ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc
   110281 acgttctgca catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa
   110341 aggtcctaag gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt
   110401 agtgatggaa gacggagaag aagtgactat tacaaataca tgtgcagcca atgtccttgg
   110461 attagtgcaa gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccatcca
   110521 agcattagct gattattgtc atacgacggt tgaagacatc gcagaacaga tcatggaaaa
   110581 atcctatgct aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa
   110641 agatcagatt tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag
   110701 taataaaatg ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg
   110761 tgtagcactt gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga
   110821 agatatacga tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc
   110881 agaatcgatc gaagtccacg tagaaatcga tccacagact tcaaaagtaa cggctatcgc
   110941 tactggttct acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc
   111001 actagagctt gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa
   111061 tacacctttc ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg
   111121 gatcatcgat caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac
   111181 aactgcagcc aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca
   111241 aactgaattg attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt
   111301 tacagcgacg gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga
   111361 accggaggaa gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt
   111421 tactaaacga attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag
   111481 atttattttg gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg
   111541 attgtggtat cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag
   111601 aaatttgtga acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac
   111661 gaatcacttt tgcaacttat gcagaagatg aagggattcg attgatgacg gagccgttag
   111721 aaaaattaaa atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta
   111781 ttggacatga actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg
   111841 aaaaaattgt gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga
   111901 gcgagatagc agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg
   111961 tattagaggc agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag
   112021 aaataaaaaa aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact
   112081 gtgacaaaag tcgtgtcaca gtcctttttt tccgaataaa cgatgttctc ctgtcaactc
   112141 ctcggtcaca agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
   112201 gtgactcgga gatgaacggc aggctcacga cctcttggaa aacggtgttc aaaagcttgc
   112261 atctgcggta ataagtccag ccaagttaaa aacataagaa gctattttta cttggctgtt
   112321 cttattactt gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc
   112381 tgaccctcgg tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttct
   112441 ttctctaata ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct
   112501 gaatatcttc aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca
   112561 tatgtaaatg catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt
   112621 gaagcattgt ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac
   112681 gtaaccatct gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc
   112741 gttttccagc aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt
   112801 ttttattttg agcgattact gcctgatcgg tttgttctag tattttttct agtttagctt
   112861 gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc aacttttccc
   112921 gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact
   112981 gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct tgaatcggtg
   113041 tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt ccaggtgcta
   113101 actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga
   113161 taaccattta accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa
   113221 atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag
   113281 cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt ttgacacgac
   113341 cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt
   113401 ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa
   113461 cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata
   113521 caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga agcgtacgct
   113581 tggaataact gatctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca
   113641 gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc
   113701 acaatttcgt agctaaaatc caaccgtgtc cccgtatact tttggatagt tggatggatg
   113761 ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg
   113821 atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct
   113881 acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct
   113941 gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg
   114001 ttttccattg attttttaca aaaataattc atccatttcg gaagtgcttc gctgatttgt
   114061 gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa
   114121 atagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc
   114181 ccagctaaag tgtcacccat gccgcccgtt gccatagcag cagaacctaa aggattggtg
   114241 tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg
   114301 atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc
   114361 aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa
   114421 agatgattag cagcaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt
   114481 gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg
   114541 ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc
   114601 caaactacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca
   114661 gttactacag tcgtgagacc cgctccgctg tgtacggcag ctaaagcact cataatgatt
   114721 gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt
   114781 gaaatttttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatctaa
   114841 aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg
   114901 aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac
   114961 ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt
   115021 aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgac
   115081 tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg
   115141 ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat
   115201 attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct
   115261 gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt
   115321 gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc
   115381 attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa
   115441 gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt
   115501 ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct
   115561 ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt
   115621 cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca
   115681 gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg
   115741 attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca
   115801 agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa
   115861 ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt
   115921 tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat
   115981 gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat
   116041 ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg
   116101 gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca
   116161 atggatgatc cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa
   116221 ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct
   116281 tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt
   116341 cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa
   116401 taattaatta aaaagaggat caagaattgc aaattatttt tgcagtcttg atcctttttt
   116461 tactttctac tgtggtattc atgataaaca tgatatagat tcaattcata ttctttctta
   116521 gcgttcgtga cgactgtatc caaaatcaac cctgttacta atgaaagaag tgctaaagtc
   116581 atcaatccag tacttaaaat cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat
   116641 tcgcgaatca caggaatccc cgtgaccaat ccaaataaaa agaagaagat ggtccaaatc
   116701 ccaaagaaaa gcaaaggctt ataatcttta aacattttaa taatcatcat gatcactttg
   116761 aatccgtcag agaatgtatt caattttgat tcgctgcctt caggacgatc acgataatcg
   116821 attgggattt caacgaactt gaatttttta tctaaagcat ggatcgtcaa ttccgtttcg
   116881 atttgaaaac cagaactcat aataggaaaa cttttgacaa agatacgatt gaatcctcga
   116941 tagccggtca tgacatcaag aatttttgtt ttatacattc tagtaattgt atttctaaca
   117001 aggttatttc caaaatcgtg gaaaggtctc ttattctcat caaagtaggt tccatttgat
   117061 aggcgatcac caatgaccat atctgcttca ccagaacgta atttctcgag caaaccatgt
   117121 acagcttctg caggataagt atcatcgcca tctaccatta aataatagtc ggcatcgata
   117181 tcaaagaaca tttggcggat cacatttcct tttccttgac gaggttcttt ttttacgatt
   117241 gctccacctt gaacggccaa ttcgtatgtt cgatcggtcg aattattatc atagacataa
   117301 atatctgctt ctggtaattc tcgtttaaag tctgcaatga ctgttgaaat tgttgcttcc
   117361 tcattgtaac aaggaatcag aacagcaatt ttttcttgtt gttccatttt tcactctcct
   117421 taactataga aaacaaaagc taaattaaaa atacctcata atcaaaggaa atgctagttt
   117481 ttcccgctaa atatcattta tttaaaaaga aaatactgga atatatgttt ggaaagctct
   117541 atctaaaaaa ccacccacta caaaagaagg acatgatacc tctcttttga atagtgggtg
   117601 cgtattcgtt ctctcacttt tttgacgtat taatctgttg cacctttttt tcttgaaatt
   117661 tctaatacgg ttcctgtatg agcatctgct aaaaattcat aataaacagt ttcgccatct
   117721 tctattcgag aaagacctcc ccgataagca ctcgaccgaa cggcgaattt cctaataggt
   117781 tccttttcaa aagagatcca agaaccttcg attgcacctt cttttagaaa agatgacttg
   117841 atattttcaa gtacttggtc tgctgacaat acttttttgc gatgatacca aatcgttgaa
   117901 gctaccccgc tcaataatcc tagaccgatt ccaactgcta acccgccttt aaaatagctt
   117961 agttcgcttt cttcatacat gtggacatac ctccttcttc tcacttgtta ctccttgtgc
   118021 ttattttacc acactctcat attttctttg aaataaataa ccgctaccag aaaaaataag
   118081 ctataataaa tacgatcggt ctgcagaaaa aatcatattt tatccgcaga tcaatataag
   118141 tctgagagga gtttagtctg aaatttagac gaatagcata tggaagaaaa aacatttcaa
   118201 cgaatcaaag agttaacaga attacaagga acaagcggtt ttgaacaaga tgttcgcgct
   118261 tatatggaaa gtcatatgga accgctggta gatgagttac agttagatgg tttgggcggc
   118321 atttttggtc tgcgccatca tgaagaagcg gatgcacccc gtgtaatggt cgcagcacat
   118381 atggatgaag tcggattcat gctgacacaa atccaagaaa acgggctttt taaagtcgta
   118441 cctctaggcg gctggaatcc ttacgttgtt tctgctcagc gttttacact taaaacctct
   118501 actggtaaga attatccatg tatttcttct tccgttcccc cacatttatt gcgaggaaca
   118561 agcggacaaa aatcagtaga agtaaccgat attttgttcg atgctgggtt tgaatcaaga
   118621 gaagaggcaa tgagttacgg tgtccttcca ggagacacaa tcgttcctta cgctgaaacg
   118681 atcaaaacag ccaacggaaa gaatattatc agtaaatcat gggacaaccg ctatggctgt
   118741 acaatggtat tagaagcttt agaagcgttg caaaatgaaa cattaggtca tacattgatt
   118801 gcaggcgcga atgtccagga agaagtaggc ttgcgtggtt ctaaagcttc tgtcaataaa
   118861 ttcaagccag atctgttctt tgcagtagac tgctctgcag cagatgatac agtgacgaaa
   118921 aatggcacat tcggacattt aggagaaggt acattgatgc gtatccaaga tccaggtttg
   118981 atcatgcttc ctcgtttgcg cgagtacttg ctggatatcg ctgaaacaaa caatatccct
   119041 tatcaatact ttgtttcaaa aggcggtaca gatgcaggtg ctgctcatac acaaaacgaa
   119101 ggaatcccaa gtacagtgat tggcgtagtg ggacgttata tccatacgca tcaaacgatg
   119161 ttcagtattc gcgattttga agctgcacga gaaatgttga ttcaaacact gaaaggtttg
   119221 gataaatcaa cagtaaatac gatcgtatac ggaaaataat gcgatagtga aactataaaa
   119281 tctggcaaaa gattgctttt tgccagattt tttttataaa aagaggatca atgtttgtta
   119341 gttcgaaaga aaggtgtata atttaattga aaaatacata aacagaaagt agaggacaaa
   119401 gaatgattat cccaaatagt ttagaagaat tagcaggcta cgtagaaact ggaaagagtg
   119461 tatttttctt tactgccggc tggtgcggag actgccgttt tatcaaaccg caaatgcctg
   119521 aaattgaaaa tgacttttct gattggcaat ttattgaagt agaccgtgac aagtacatag
   119581 atgtagccgc tgaatggaac atttttggta tcccaagttt tgtcgtgatc caagacggaa
   119641 aagaattagg tcgtctagtg aataaagatc gtaaaacaaa acaggaaatc gaatcgttcc
   119701 tagaaagtgt aacaaaaggc tgacatctgt taggaggggt caagatgaat caagaatata
   119761 agaatatttt agtaggtatc gacggtagtg aacaagcact tgctgctttc aaaaaagcgg
   119821 tagaagtggc tcgccgtaat aatgggaaag tatatgtagc aaacgtaatc gatcagcaat
   119881 tttataactt tatgggctat gcacctattg atcaaaatct agtcgatcag caaactgaaa
   119941 cagccaagga aatgatcgaa gaatgtaaag aatatgggaa atctgttgat tacacaaata
   120001 tagaaggaat tattgcatat ggttctgttc gtgaatccat ggcagtcaaa ttgccaaaaa
   120061 aatatgagat cgatctaatc atggttgggc agtcaggtct aaatgcagtc gaacgcttta
   120121 tgacaggaag tgtagccagc tacgtgatac gtcattcacc ttgtgatgtc ttgatcgttt
   120181 ctgacgaaga agaacagcca agtaattaag caagtggaac ttgtacgacc tttttaatca
   120241 attaactaaa acagtaaagg gactgtgaca aaagtcttgt cacagtccct tgttacgaat
   120301 aaatggtgtt ccagaagagg cttcttcgga aataaagcat gaaaaaaatt tcatgcagtg
   120361 aatgcaattt tttgttaaga taaatctcag caaaggataa tggaggaaag aacatgattt
   120421 ttgcttataa caaagcacac gtcggagaca ccttgatggt tattatagcc gacgataaag
   120481 gaacagaaaa cactgttgaa cgcaaatgga atgttgctcg gatcaaagat gaacaaggtc
   120541 aagttgttgc ttggaacttt ttccatatct ccgatcatct aacaatcgaa gggaacggcc
   120601 aagtaagctt aacagatgag gaattagcga aattgaatcg attgatcaaa gaagcaggat
   120661 tcgaagaaca gcttgaagct gatcatgaac cgaagatcgt tgtcgggtac gtaaaaacat
   120721 gcgtcccaca tcctgattct gaccatttat cgattactga gacagaagta gacaatggta
   120781 cagtattgca aatcgtttgc ggtgcaccca atatcgaagc tggacaaaaa gttgtcgttg
   120841 caaaaccagg agctatgatg ccagatggaa tgatgatctg gccaggaact ttgcgagggg
   120901 tagatagcta tggaatgatc tgttccgctc gtgaattgca tttgccaaat gctccagcaa
   120961 aaaaaggaat cttagtattg cctgaggatg ccgtagtcgg agaagcattt ccaaaataaa
   121021 gaggttgtga caagtctttg tcacaacctc ttttttatta attttcacta ttttgatttt
   121081 gttttaatgc cgattgatcg atcgtcaatt caacagtagt tgttttttct ttcgagcctt
   121141 catagtaagt gattttgaca gaatctccca cttttttctt atacaaagca gattgaagtt
   121201 ctacaccaga agaaacttct ttgtcatcga tcttagtaat gacgtcgtat tgtttcaatc
   121261 cagctttttc agccggtgta gctgtttgga catttgtcac gatgacaccg ttagttacag
   121321 atgaaggaat cttcaagatt tgttcttgtt gctgagtaga aacggcagat aagtcgacca
   121381 ttgtgatacc tagagctgga cgtgtcactt ttccgtcttt ttccagttga ttgatgatat
   121441 tcactacatc gttacttgga atagcaaatc ccattccttc tacgcttaca ttagaagtag
   121501 attcagaagt acttgcgatc ttacttgagt tgataccgat aacttgtcct tcgatattaa
   121561 ctaaaggacc accagaattt ccagggttga ttgctgcatc tgtttggatc gcattgatat
   121621 tgactgtttc gttcgattca ttcgtacttg tcacttgtcg gttcaaggaa gaaatgattc
   121681 ctgaagtgac agaatttgca tattcagatc ctagtgggga accaatcgca atcgcaggct
   121741 caccaacctt cagagcactt gagtcaccaa aagaggcaac tgtttcgact ttgtctgaat
   121801 tgatcttcaa caccgctaaa tctgaataag catctgttcc aactaattca gcttttacct
   121861 tcgtaccgtc tttcatcaag acttcaagac cttgctggcc atctactacg tgattattcg
   121921 tgactacata agcagtattc ccgtcttttt tgtagatcac gccgcttcct tcactataag
   121981 cttccaaatt actatcatct gagctgctac tttcttcttg ctgcccgaat agtccgccaa
   122041 aaccgcttga ttggttttga ctttgtaagt tgataacaga aacgacagcg tcttgaactt
   122101 tatctaccgc tttggtgata tcagaatcta cattgacttt gacattttcc acaactgttt
   122161 caccggcaga attctgattg ccgcttgtag cagtagaact attgttgcca gtgcccatcg
   122221 ctgcataaaa aatgccagct gtcagtagtc ctccaacgat ccctccaaca agccccagac
   122281 ctaacttacg ccagaggcta ttatttttac tttttttcat ctttggtgtc acattttttc
   122341 tatccatgtg ttcctcctcc aattacattc atttatctac tgttagtata gtcttatttg
   122401 ttgactttag tctactttga gactgtgaac tttttatgaa aaactttagg aaaaatcatt
   122461 gttttcagat gaaaatattt gattttcttt ttccacagat actgtgtaaa actacgcgga
   122521 taggattgga aaaataaggg ataaaacata tccacagcct gtgtataaag tggataaatt
   122581 cttataaaac gtatgtttgc ctaatgaatg tgatgaaata aggtaaaata acactgtttt
   122641 ctgtggataa acctgtggat gttgtgcata actgttgggg aaatcttttt aacaaaaata
   122701 gccaaaataa acatatagac gaaaaaattg attataataa acaaagtgag catgaagacc
   122761 aaaggagaga aaacatgaat atcaaaatta tctcagtagg aaaattaaaa gaaaaatacc
   122821 tgatccaagg tatcaatgag tatgtcaaac ggctgaatgc ctatgcaaaa atcgaattga
   122881 tcgaagttcc tgacgaaaaa gccccggaga atttaagcga agcgcaaatg agacaagtaa
   122941 aagaaaaaga aggggaacgg atcttagcga aaatcaaaga acaagaatac gtctatgcct
   123001 tagcaattga agggaaaaat cctacaagtg aatcttttgc taaacaaatc gaccaattag
   123061 gtatccaagg aaaaagccac cttgtttttg ttatcggcgg ctctttagga ctcagcgaag
   123121 ccgtcatgaa aagaagcaat gctcagatct cttttggaaa aatgacgtat ccacatcaat
   123181 tgatgcgtct gatccttgtc gagcaaatct accgtgcctt ccggatcaat gcgggggcgc
   123241 cttatcataa gtgagacaag aagaatgaaa gatagtaaag tagtagtaaa tagtcatatc
   123301 caagaaagcg gatatggctg ttttttttat gaaagatgtt tgtttcgcta aagagatcgc
   123361 aatccacgct cggtaaatat aagagccaca taattaagct ttgaactcat tagttaagca
   123421 attcgatgtt ggaacaattc ttggtgttca gaccattacc tccctctctt ataattttag
   123481 tgctttttta agtgagagta aaaataaata acgatttatt taaaatttaa gttatactag
   123541 ataaagcaat ccatgaaagg agtatataat taagtaatgc aagtaaagaa aaaaataata
   123601 ttgtccattc tcctagcaat tattgctgga ctttattcaa tcaattattt tagaaatgtt
   123661 catactattt ctacatcggg ggatattgcc ttccattttg ctagagtcaa agggttatct
   123721 tcaatttttt ctggtccaat caattttacg acgtttaatc attatgggag cggagtgaat
   123781 tatttttatc cctacttgac cttttttccg gcagtcattt tttattggat aagtaataat
   123841 ttaatcgtta gttatatctt atatgtttgg ctattgaatg tttgtactat catgctgatg
   123901 tttcattatg gactaaaatt tttaaaaaga atcgatgcgg cttttatatt tagctgttta
   123961 tatacatttt atgggtaccg tacgattgat atttatcatc gttctgctat tgctgaagcg
   124021 attgcactta cggttatacc gatagttatg tattatgctt acgcactgat ttatgagaaa
   124081 aagccatgtg caattccatt agcagtcagt atgagcttac tcatttatac acatgttctt
   124141 tccactttta tgagtgtaat agccgttggc ttactagtct taggcggctt tgtagtaagt
   124201 gaaacaagaa aaaaagaaag catccggttg attgtggagt tgtcaaaggc agtaggaatg
   124261 acgattcttt taacagcata cttttggtat ccgatgttcc gacaaatcat ggttcaatcg
   124321 attaatgagc cagataaaac gaccttacaa caccaagcat taaatgtctc ggatagtttg
   124381 attggtgcga tgaataatga cctgacaacc tttacggtag gaatcattgg tattatttct
   124441 ttgatctgtc cactcttctt ttttaaagga tttaatcgca aagaaaaagt gatttacctt
   124501 agtgcggtag tctcttggct cttatccacc aaattttttc catggtcttt attacaaaat
   124561 acacccattc aaatgattca atttccttgg cgaatcttag gctttcaagt actgtttggc
   124621 tcgttgattt tatcgttcgt tttcatgaag agcacatcag ctaagcaaag aaaagtagtt
   124681 aatataacgg caattgtttt attactgttg gctgtatctg ctgcaacaaa agcaaactat
   124741 gaacataaag ttgtcactca gagagatcac ttggtaatga atcaagaaaa tcttattcgt
   124801 tacacgacac tccttcccgg aggactttat gactatgcac ccacggaagc attacaatac
   124861 aaagaacatt tgcagaatca tgaagtaaga attggcaagg aatggcatac tatgccttat
   124921 caagtgatgg attcgaaaat agtctataca gtacatgaat ctaaaggaca aaaaataacg
   124981 cttcctgtat ttgattattg gggaacagtc gcaaaagtaa atggacaaaa aagagagatt
   125041 gataatagga ctagtgtcga ttgaaggaac acaaggagaa acaatcattg aagtttcttc
   125101 catttatcct ccaactatga agttagcttt tttaattagt ttatgtagtt acctctttct
   125161 tttaattagt tttcttatca ggaagttaca acatataaaa ataatgatct gattacattt
   125221 ttttcaatat tagcttacta gagaataaga aaaagtaagt aataaaccaa atttaaactt
   125281 tatattataa taatgaagct taatcttctt tatagagaat tgggctattt ttaatggcaa
   125341 agaggggaag aagatgtttc aaaacaaagg acaacgctat gttgtaaaag gagtaaagga
   125401 atcttactgg aatcaaaaat ctttattgag aaaattatct ttgaatttaa gcacaaagaa
   125461 atgaaggtac actagatagc aatgaagata tctttgtcat taaacgtcgc tattttacac
   125521 aagtaagtga tgatttgatt catgtctttt ccaatgagtg gtttgtttct gagaaaaaat
   125581 tacatgcttc taatctgcaa tatatgccag gtgaagatcc aattcctaat atgaaagcaa
   125641 ttatcaacag taaggattac gaaggatata aagccaaaca tccagaagcg aaaccattta
   125701 aatacccaca agaaatgaag cgagcatggc gtaaaatgtt agatgatgag ctgattcccc
   125761 ttgaaaatga attgaggtaa acgagaagaa ttcaataaac tggatgataa aaatataata
   125821 tgactataaa ttggttacag cttcaaccta taagttgtaa taaattagcc taaaagtaat
   125881 ttattaatga tgaggtgaat ttgattggta caacaagata tatcacttag agagggagaa
   125941 gcagcactac aaactacttt tcaataagta ggaattatag aaattttact caaaaaatct
   126001 tcagtaaaca atttggctaa ataagattca gttgttatac gagaaggtta cggttcttct
   126061 taactaagct tagtatgttt ttattcaagg catgattttc tctatgtgat cattcagttt
   126121 ctgtaagagt tctaataatt cttttttgga gtacttgtaa ttgctttcta cccaccgatt
   126181 gagaacggat agacaacctt ggacgtggta cgtagtaaag aatctcattt cttctgtgat
   126241 aatcgttttc ttgtcttcat atagccatat gttcagatag ttttcttcaa taacttgttt
   126301 aactttcttc tgaaacgaaa tatcaccatg gttgaggata aataagcgat ataaaaaagt
   126361 atttgcgtaa acatagtcaa tcaagcgagt gtatagttcc tcatagctat gggctggatc
   126421 ataagagaga atttcattta gatcccgaaa aaagttgctt tctaattgat
//
LOCUS       DAZKMZ010000003        76010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000003 DAZKMZ010000000
VERSION     DAZKMZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.003"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..645
                     /locus_tag="ACG8M5_000273"
     CDS             13..645
                     /locus_tag="ACG8M5_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357558.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(779..904)
                     /locus_tag="ACG8M5_000274"
     CDS             complement(779..904)
                     /locus_tag="ACG8M5_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0357559.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1038..1931)
                     /locus_tag="ACG8M5_000275"
     CDS             complement(1038..1931)
                     /locus_tag="ACG8M5_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0357560.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2113..3039)
                     /locus_tag="ACG8M5_000276"
     CDS             complement(2113..3039)
                     /locus_tag="ACG8M5_000276"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0357561.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="ACG8M5_000277"
     CDS             complement(3124..3885)
                     /gene="pflA"
                     /locus_tag="ACG8M5_000277"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0357562.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="ACG8M5_000278"
     CDS             complement(4129..6360)
                     /gene="pflB"
                     /locus_tag="ACG8M5_000278"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0357563.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6637..9087)
                     /gene="parC"
                     /locus_tag="ACG8M5_000279"
     CDS             complement(6637..9087)
                     /gene="parC"
                     /locus_tag="ACG8M5_000279"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0357564.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9099..11144)
                     /gene="parE"
                     /locus_tag="ACG8M5_000280"
     CDS             complement(9099..11144)
                     /gene="parE"
                     /locus_tag="ACG8M5_000280"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0357565.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11310..11744)
                     /locus_tag="ACG8M5_000281"
     CDS             complement(11310..11744)
                     /locus_tag="ACG8M5_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0357566.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11871..12509
                     /gene="plsY"
                     /locus_tag="ACG8M5_000282"
     CDS             11871..12509
                     /gene="plsY"
                     /locus_tag="ACG8M5_000282"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0357567.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12641..13516)
                     /locus_tag="ACG8M5_000283"
     CDS             complement(12641..13516)
                     /locus_tag="ACG8M5_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0357568.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13598..14389)
                     /gene="codY"
                     /locus_tag="ACG8M5_000284"
     CDS             complement(13598..14389)
                     /gene="codY"
                     /locus_tag="ACG8M5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0357569.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="ACG8M5_000285"
     CDS             complement(14407..15807)
                     /gene="hslU"
                     /locus_tag="ACG8M5_000285"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0357570.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="ACG8M5_000286"
     CDS             complement(15821..16369)
                     /gene="hslV"
                     /locus_tag="ACG8M5_000286"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0357571.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="ACG8M5_000287"
     CDS             complement(16406..17311)
                     /gene="xerC"
                     /locus_tag="ACG8M5_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0357572.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="ACG8M5_000288"
     CDS             complement(17509..18885)
                     /gene="trmFO"
                     /locus_tag="ACG8M5_000288"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0357573.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18854..20932)
                     /gene="topA"
                     /locus_tag="ACG8M5_000289"
     CDS             complement(18854..20932)
                     /gene="topA"
                     /locus_tag="ACG8M5_000289"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0357574.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="ACG8M5_000290"
     CDS             complement(21054..21911)
                     /gene="dprA"
                     /locus_tag="ACG8M5_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0357575.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21967..22734)
                     /locus_tag="ACG8M5_000291"
     CDS             complement(21967..22734)
                     /locus_tag="ACG8M5_000291"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0357576.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="ACG8M5_000292"
     CDS             complement(22727..23587)
                     /gene="ylqF"
                     /locus_tag="ACG8M5_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0357577.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23847..24245
                     /locus_tag="ACG8M5_000293"
     CDS             23847..24245
                     /locus_tag="ACG8M5_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0357578.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24482..25024
                     /locus_tag="ACG8M5_000294"
     CDS             24482..25024
                     /locus_tag="ACG8M5_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0357579.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="ACG8M5_000295"
     CDS             complement(25107..25658)
                     /gene="lepB"
                     /locus_tag="ACG8M5_000295"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0357580.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25810..26037)
                     /locus_tag="ACG8M5_000296"
     CDS             complement(25810..26037)
                     /locus_tag="ACG8M5_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0357581.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="ACG8M5_000297"
     CDS             complement(26034..26552)
                     /gene="msrA"
                     /locus_tag="ACG8M5_000297"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0357582.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26572..27285)
                     /locus_tag="ACG8M5_000298"
     CDS             complement(26572..27285)
                     /locus_tag="ACG8M5_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0357583.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27288..28184)
                     /locus_tag="ACG8M5_000299"
     CDS             complement(27288..28184)
                     /locus_tag="ACG8M5_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0357584.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28342..29184)
                     /locus_tag="ACG8M5_000300"
     CDS             complement(28342..29184)
                     /locus_tag="ACG8M5_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0357585.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29316..29969
                     /locus_tag="ACG8M5_000301"
     CDS             29316..29969
                     /locus_tag="ACG8M5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0357586.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30032..30559)
                     /locus_tag="ACG8M5_000302"
     CDS             complement(30032..30559)
                     /locus_tag="ACG8M5_000302"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0357587.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30579..31526)
                     /locus_tag="ACG8M5_000303"
     CDS             complement(30579..31526)
                     /locus_tag="ACG8M5_000303"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0357588.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31538..33421)
                     /locus_tag="ACG8M5_000304"
     CDS             complement(31538..33421)
                     /locus_tag="ACG8M5_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0357589.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33584..35662)
                     /locus_tag="ACG8M5_000305"
     CDS             complement(33584..35662)
                     /locus_tag="ACG8M5_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0357590.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35675..35950)
                     /locus_tag="ACG8M5_000306"
     CDS             complement(35675..35950)
                     /locus_tag="ACG8M5_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357591.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36312..36767)
                     /locus_tag="ACG8M5_000307"
     CDS             complement(36312..36767)
                     /locus_tag="ACG8M5_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357592.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36921..38129)
                     /locus_tag="ACG8M5_000308"
     CDS             complement(36921..38129)
                     /locus_tag="ACG8M5_000308"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0357593.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38241..38573)
                     /locus_tag="ACG8M5_000309"
     CDS             complement(38241..38573)
                     /locus_tag="ACG8M5_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0357594.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38721..39596
                     /locus_tag="ACG8M5_000310"
     CDS             38721..39596
                     /locus_tag="ACG8M5_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0357595.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39678..40259)
                     /locus_tag="ACG8M5_000311"
     CDS             complement(39678..40259)
                     /locus_tag="ACG8M5_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0357596.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40265..41524)
                     /locus_tag="ACG8M5_000312"
     CDS             complement(40265..41524)
                     /locus_tag="ACG8M5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0357597.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41526..42572)
                     /locus_tag="ACG8M5_000313"
     CDS             complement(41526..42572)
                     /locus_tag="ACG8M5_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357598.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42807..43082)
                     /locus_tag="ACG8M5_000314"
     CDS             complement(42807..43082)
                     /locus_tag="ACG8M5_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0357599.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43325..44635)
                     /gene="der"
                     /locus_tag="ACG8M5_000315"
     CDS             complement(43325..44635)
                     /gene="der"
                     /locus_tag="ACG8M5_000315"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0357600.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="ACG8M5_000316"
     CDS             complement(44817..46046)
                     /gene="rpsA"
                     /locus_tag="ACG8M5_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0357601.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46181..46861)
                     /gene="cmk"
                     /locus_tag="ACG8M5_000317"
     CDS             complement(46181..46861)
                     /gene="cmk"
                     /locus_tag="ACG8M5_000317"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0357602.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46910..47545)
                     /locus_tag="ACG8M5_000318"
     CDS             complement(46910..47545)
                     /locus_tag="ACG8M5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0357603.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47611..49014)
                     /locus_tag="ACG8M5_000319"
     CDS             complement(47611..49014)
                     /locus_tag="ACG8M5_000319"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0357604.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49001..50032)
                     /locus_tag="ACG8M5_000320"
     CDS             complement(49001..50032)
                     /locus_tag="ACG8M5_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357605.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50063..50284
                     /locus_tag="ACG8M5_000321"
     CDS             50063..50284
                     /locus_tag="ACG8M5_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0357606.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50449..51186)
                     /locus_tag="ACG8M5_000322"
     CDS             complement(50449..51186)
                     /locus_tag="ACG8M5_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0357607.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51339..52679)
                     /locus_tag="ACG8M5_000323"
     CDS             complement(51339..52679)
                     /locus_tag="ACG8M5_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0357608.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52916..54439)
                     /locus_tag="ACG8M5_000324"
     CDS             complement(52916..54439)
                     /locus_tag="ACG8M5_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0357609.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54817..55428)
                     /locus_tag="ACG8M5_000325"
     CDS             complement(54817..55428)
                     /locus_tag="ACG8M5_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0357610.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55551..55676)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55758..56474)
                     /locus_tag="ACG8M5_000326"
     CDS             complement(55758..56474)
                     /locus_tag="ACG8M5_000326"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0357611.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56480..57049)
                     /gene="scpB"
                     /locus_tag="ACG8M5_000327"
     CDS             complement(56480..57049)
                     /gene="scpB"
                     /locus_tag="ACG8M5_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0357612.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57033..57803)
                     /locus_tag="ACG8M5_000328"
     CDS             complement(57033..57803)
                     /locus_tag="ACG8M5_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0357613.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57800..58171)
                     /locus_tag="ACG8M5_000329"
     CDS             complement(57800..58171)
                     /locus_tag="ACG8M5_000329"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357614.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58190..59077)
                     /gene="xerD"
                     /locus_tag="ACG8M5_000330"
     CDS             complement(58190..59077)
                     /gene="xerD"
                     /locus_tag="ACG8M5_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0357615.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59173..59646)
                     /locus_tag="ACG8M5_000331"
     CDS             complement(59173..59646)
                     /locus_tag="ACG8M5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0357616.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59751..60614)
                     /locus_tag="ACG8M5_000332"
     CDS             complement(59751..60614)
                     /locus_tag="ACG8M5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0357617.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60810..62909)
                     /locus_tag="ACG8M5_000333"
     CDS             complement(60810..62909)
                     /locus_tag="ACG8M5_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0357618.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63129..64238)
                     /gene="rpoD"
                     /locus_tag="ACG8M5_000334"
     CDS             complement(63129..64238)
                     /gene="rpoD"
                     /locus_tag="ACG8M5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0357619.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64262..66103)
                     /gene="dnaG"
                     /locus_tag="ACG8M5_000335"
     CDS             complement(64262..66103)
                     /gene="dnaG"
                     /locus_tag="ACG8M5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0357620.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66285..68600)
                     /locus_tag="ACG8M5_000336"
     CDS             complement(66285..68600)
                     /locus_tag="ACG8M5_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0357621.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68785..69645)
                     /locus_tag="ACG8M5_000337"
     CDS             complement(68785..69645)
                     /locus_tag="ACG8M5_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0357622.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69704..70336)
                     /locus_tag="ACG8M5_000338"
                     /pseudo
     CDS             complement(69704..70336)
                     /locus_tag="ACG8M5_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70503..71264
                     /locus_tag="ACG8M5_000339"
     CDS             70503..71264
                     /locus_tag="ACG8M5_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0357623.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71290..73881
                     /gene="mprF"
                     /locus_tag="ACG8M5_000340"
     CDS             71290..73881
                     /gene="mprF"
                     /locus_tag="ACG8M5_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0357624.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74069..75256)
                     /gene="tuf"
                     /locus_tag="ACG8M5_000341"
     CDS             complement(74069..75256)
                     /gene="tuf"
                     /locus_tag="ACG8M5_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0357625.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75624..75980
                     /locus_tag="ACG8M5_000342"
     CDS             75624..75980
                     /locus_tag="ACG8M5_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0357626.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20748 a        16020 c        13051 g        26191 t
ORIGIN      
        1 tttggagata ttatggaaat tcaacgtaga accactaatt ttttacattt aggctctttt
       61 tttgcaactt tgacgatctt attttttcta tgttattcat accttaagac ctcacatgcg
      121 ccttctagtg cattaggctg gttgcttttg gatcattctg atatcacaga agccgaagaa
      181 aatccgtttt ttttgatttt gtcttctctt tgcggcttac tgttttctgt ttatttcggt
      241 gcctattttt ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt attgattgca
      301 tttaaaacgt tttctttttt agcaattctt ttttattcgt ttaatcacaa tacgcttttc
      361 ttctttatca tgaatggttt agtcgtgatt ttatccattt ttacttattt tattttttca
      421 ctcattttta gccaattgaa atgtccgctc ttctttcgat tagttcctgt cacctgtttt
      481 atttacagct tgttttcctt ttttgtcctt gctttaatgc cactgaccaa cccttcgacg
      541 atccaagtcg caggaaatct gttggatatg ctcttcatcg tcagtctagg tgtatttatg
      601 tggcaaagaa aaaacaaaca aaataaaaag cattttgaag cctgactatt acgacttcat
      661 aaatgctttt ttagttttaa acacttcaat ctaaaagaat aacccaagat tggataaatt
      721 attaatgact cgccattcgt ttcttagtga ttctggatta tcaaatggac ttcctaatct
      781 aatcagctag ttcttcccat aaaatgccat acttgactga atcataatat tgattattta
      841 aaaatctaac ttttcttatc actacttcct ttgtcattcc acatttctca ccattttttt
      901 gcattccttt gtttccagac catgtagtga atctaatagg tggtagatag gcaaaatact
      961 caaataattc ttttaaccat aaaggaagag aaaggctcct cctgcaagag tcagtataat
     1021 ttttgcttac tgctctctta ggttttactc actaaaactg ttccatattc tttgatataa
     1081 gaaaatgcat gcggtataaa tttcttgctc agctcagaaa agatttgtcg tttaagaaag
     1141 gctatttcct ccattgtcaa tgaaggatcc aatgttggta attcaatctc aaaattgttt
     1201 ttttcgacaa taggtaattc taatgtgaat gattcttctt cctttatagt attcgtcaca
     1261 agtagagact gatggaaaat actaggccgg cgatttttat atttattctt acgtatcctg
     1321 tcaacgataa cttctatttg atcaaaaaag tacgattcat ttacgtctcc aataagttcg
     1381 gctaattctt tgaatgcctt cgttacagga agctgattct tctgtacaaa aaagtaatta
     1441 gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga taaaacggta cttctcattt
     1501 cctgcagttg tcctcagcaa ttctgtctct cggctgtttc ttactccata aaaatacgct
     1561 tctttccgtt catcttcgaa acaatacgcc caaataactt ctcccatcgc taatttttgt
     1621 tcatcaacag agagtctctt caattgttca gctatggttt cagcagccga agtggcttgt
     1681 tgcaagtaat ctttaggatc aaatattgga aagttcaaat gatatcttcg ttcttccctc
     1741 ttgattaatc ctgcatcgat caataaatca agaaaatggg aaaatttatt ttgcgaaaat
     1801 tcttttttca attctcttag aatcgtttct tgttctcgat gttcatatag ataatcggtc
     1861 aatgaagaaa aaagcggatc atcggtcagt ttttgataaa agttttcctt attttttgaa
     1921 taatagttca tatatgttct cttttctatc cgttttattt tacttaaaag gcttttacgt
     1981 aaaaaggagg aagaacaaga gtcataattg actgttattc tcctccattt cttcggcctt
     2041 tagtatgttc tatgtttctt tttatatcaa tcgcttatcc attcattttg aatgcttcat
     2101 actaccattt gattagctaa atacagccgt caattgaggt acgacttgtt ttttacgtga
     2161 aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag cttcttctac
     2221 tttttctttt cctttaccag caacaagtaa ttctgaatca ctgtctaaga tgtttgtgac
     2281 aactaatacg aacagatcgt agccatttgc tttcatttca tcttccattg ctgcttctag
     2341 ctctgcttgt cgatccatca cttcattaag gtccactgta ttgacttgag cgatacgaac
     2401 gttggaactg cccattggga aactttttgc atccaaatca agaagaaccg cagctgattt
     2461 atcacttaga tttgttcctg ctttcaacat ttcaagtcca taagtgttta gatcgacttc
     2521 agcgattgct gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg ttggtgattt
     2581 gaacaacaat gtatctgaaa tgatagctga gagcatgatt cctgcgattt ctttaggaat
     2641 tgttacttgg ttttctttat atagttttaa aataatcgta cttgtacatc caactggttc
     2701 tgcacgatag tacaatggat tagctgtttc aaagtttgca atacgatggt ggtctactac
     2761 agctaaaata tttacttcgc tgatatctga aatactttgt tggaattcat tatgatccac
     2821 aagcatgaca tctgcatttt ctgctttagc tgactctact actcgtggtg cagatagctg
     2881 aaagtggtct aatgcgtatt gtgtttcttc attaggtgta cctagagcaa ctgcttctgt
     2941 ttctttacct aattcatttt gcaagtgtga aaacgcaatg gctgccccga ttgcatctgt
     3001 atctggattt tggtgaccaa aaactaaaat ttttgacatg tcattcgctc ctttgtgatt
     3061 tctcctattt aataatagca aaaaaacgga aagaagcaat acttatcttc tttccgatgc
     3121 gacctatcgc gttaaatatt ttgtatactc ttctgtatga agcaatcgtt tagcattttc
     3181 gacacgttct tttgtaggtg gttcgatccc ttctaatgga tatttcaaac caagtgtttc
     3241 ccacttgtat tttcccatcg tatgataagg aagaatctct actctgtcta catttgacaa
     3301 ggtttgaata aagttgtcta gtcggatcaa atattcatca taatcacttc gctcaggaac
     3361 tagtacatgg cgaatccaaa ccggtttgtt gatttttgac aaatattgag ccatttctaa
     3421 aatattttca ttgtcccaat gcgttagttt tttatgctct tcattatcga tgtgtttgat
     3481 gtcaaataac agtaaatctg tatacttcat caattcttga aaacgtgaaa agaaaggttc
     3541 ttcatacgta aaaggttttc cgcatgtatc caatgtagta tgcatcttcc gctcttttgc
     3601 ttttttaaat agttcgatca aaaaatcgat ctgcaataga ggttctccac cactaacagt
     3661 aataccacct ttatctcccc agtaaggtct gaagcgttct gctttatcta aaagttcatc
     3721 tgctgtgtat tctttccctc cgccaatatt ccaagtatct ggattgtgac aaaattggca
     3781 acgcatgcgg catccttgca taaacacgac aaagcgcagt ccaggtccgt ctactgaacc
     3841 gaaagtttcg atagaatgaa catagccgat tgttttttct tccatttaag ttcacatctt
     3901 ttctttgatt tcattttaag tacaagttag ctgtcgaagc gaagctttct gaacaaaatt
     3961 tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg acggaacata caagtcctgc
     4021 ccctgtaaat cgtttctttc aaataagcat actgattatt cgattttgat gcgatggcta
     4081 ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca cgaacttttt acattctgtc
     4141 gtgtgaagtt ctcatcaaga catctaattg ttgttctctt gtcaagtcac ggaatttaac
     4201 tgcataacca gatacccgga tcgtcaagtt cggatatttc attggatttt ccattgcatc
     4261 acgcaataaa tcattactga atacattgac attcaaatga taagctcctt ttgagaagta
     4321 tccatctaag acattacgca agttttcgat tcttgtatct tgttcttttc caagacctga
     4381 aggattgatt gtttgggtat ttgaaattcc atccaatgca cagccgtaat tcattttagc
     4441 tgttgagttc aggctagcta ataacccact ttgttccgca ttgtaacttg gatttgctcc
     4501 tggcgcaagt ggttttccag cttcacggcc atcaggagta tttcctgtat ttttaccgta
     4561 aacaacattt gaagtaatcg tcaataatga agttgttggt tttgagttgc ggtaagtttt
     4621 atgacgtttg atttgtccca tgaagtattc caagatccat gaaactaatt catctgcacg
     4681 gtcatcatca tttccgtatt tagggaagtc gccatccact tcaaaatcaa ctgctattcc
     4741 tttttcatta cggattgctt ttacttttgc atatttgatc gctgacaagc tgtctgccgc
     4801 atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat ccatcaaagc
     4861 cagctgagct gcttcataag aatatttatc atgcatataa tgaatgatgt tcaacgtatt
     4921 gacatatagt tctgctaacc aatccatcat attcatgaag ttatgtttca cttcttcaaa
     4981 gtctagtatt tctgatgtga ttggacgata ttctggtcca acttgggcgc cagtcacttc
     5041 atcgactccg ccattgatgg catataatag tgttttagcc aagttagcac gagcgccaaa
     5101 gaattgcatg tcttttccga caactgttcc tgatacacag caagcaatcg cacaatcatc
     5161 agaaccccat tgttcacgaa gctgattatc attttcgaat tgaatcgaag aactttcgat
     5221 agcgattctg ctagcatatg ttctgaatcc ttctggaagg tgttcagaat acaaaacagt
     5281 caagtttggt tctggtgaag gtcccatatt tgttaatgta tgaagcagac ggaaatcatt
     5341 ctttgtaact aatgaacgac catctaatcc catacctgca atggataaag tagcccagat
     5401 agggaatcct gagaataatt ggttatattc aggtgtacgc gcgaatttga ccatacgtaa
     5461 tttcattact aagtggtcga tcaattcttg tgcttctttt tctgtgatta ctccattatc
     5521 caaatcacgt tggatataga tatcaaggaa tgctgagata cgtccgattg acatcgcagc
     5581 accattttgt gatttgattg ctgctaaata tccgaagtat agccattgga cagcttcttt
     5641 tgcatttgca gctggtttag aaatatcata gccatatttc gcagccattt ctttcatttg
     5701 cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt cttcactcat
     5761 tgtatgataa ccagtcatat catgatcttt tttcttttgt tccatcaaat agtcgattcc
     5821 atacaatgcc acacgacgat agtcaccgat gatacgtccg cgaccgtaag cgtctggaag
     5881 ccctgtaatg attttgtttt tacgagcagc acgcatttca gcagtataag cgtcaaatac
     5941 accttgatta tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat cgtttggtgt
     6001 atagccattg ctttttagcg cattgttagc catattgatt ccgccgaacg gcatgaatgc
     6061 ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt tccaaatctt tatccaaata
     6121 gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg tccatatcat agacaccgtt
     6181 acgctcgaat tggatatcat ttaacttttg caattcatcc cataattttt ctgttgcttc
     6241 tgttgggcct tccaaaaagc tgtcatcacc gcgatattca gtgtagttat tttgaataaa
     6301 gtctctagtg tctacgcctt ctttccattt ggtaccttta aatccattcc attgctccat
     6361 tgtatggacc tcctaaattt ctgttttatg taacagtttt tacgtgatta gtataacaca
     6421 gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa caatttttat gttttattta
     6481 catatattta aatcaacgtt gacataccaa catttaagca ttgtgaacac tttcatcttt
     6541 ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa agagacgcca cctgtattaa
     6601 tacagatggc gtctcttcat taaaatacta caacagttac gactcgatga aaatctcagg
     6661 agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa atgatccatt
     6721 cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct gccctttttg
     6781 attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac ggtgcggatt
     6841 ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt ggctgatttc
     6901 ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg gcgtatcacc
     6961 ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat tcatagactt
     7021 cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc gcaaacccat
     7081 tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca gatacacagc
     7141 aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc tcttataggt
     7201 gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct tgctgatgaa
     7261 tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa tcgattcgtc
     7321 tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc atttcaaatc
     7381 gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca gaagaacatg
     7441 gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca tcccgatttc
     7501 ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc cttctcgcgt
     7561 cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt tgatttcttc
     7621 gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca cttctcgcaa
     7681 ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat tcaactctgt
     7741 gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg tgttcgtcaa
     7801 acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt gaaagacgtc
     7861 gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg cgattacttc
     7921 atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt tttgagcttt
     7981 ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga tataacttct
     8041 taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga ccatattgaa
     8101 attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc cttgagcatt
     8161 ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt catctcgtac
     8221 ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt tcaccaaggt
     8281 cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat tacccttcat
     8341 tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt cataagcttt
     8401 tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc caggaatata
     8461 ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg tgccatcgat
     8521 cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata tacctgtcga
     8581 tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt tttctgtatc
     8641 atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca tctctccact
     8701 taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac cgtccatact
     8761 cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt ggctcatgcg
     8821 gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca tgatatttcc
     8881 gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt tgttcatggc
     8941 aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca gtgcacgttc
     9001 ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt ctaaagttaa
     9061 ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc aaataactga
     9121 ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac tgaaggagag
     9181 atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt aaattgcacg
     9241 tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt ggttactcga
     9301 cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga agtcggatcc
     9361 atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata tcgctgcagc
     9421 atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt ccacgcatat
     9481 tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg taacgcgata
     9541 tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt aagcagtaat
     9601 acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt gtcgtaatta
     9661 cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat catcgtattg
     9721 atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat gacttttcct
     9781 cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga tccgccagca
     9841 gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg tgcgggtgtc
     9901 aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg gctttcttct
     9961 ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg gaccaacatc
    10021 tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat gacgttatct
    10081 actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc aaattgcaga
    10141 agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa atcactacct
    10201 tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc attataagat
    10261 ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt ccgaacgtta
    10321 ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc gacctctact
    10381 tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc tttttcttca
    10441 ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc cttgactggt
    10501 tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa ggcagattct
    10561 cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat tgtatcatct
    10621 ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt tttcaaagta
    10681 ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc tctaacgatc
    10741 gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc gtgcagtcca
    10801 ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac agtaaaaatc
    10861 acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg tccgctatct
    10921 gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc ataacctgat
    10981 aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa gcctcggctg
    11041 tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc ttccaatact
    11101 tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa cgctccttca
    11161 ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc gtgaaaaaaa
    11221 agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga cattcccctc
    11281 tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa cttaccaagt
    11341 tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct tagcatctca
    11401 gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc agttttcaaa
    11461 aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac gatatcgatt
    11521 ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc ttcaccagcc
    11581 aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg tgcaaccatg
    11641 taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt tgcttcttcc
    11701 aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc caccccttgc
    11761 ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg ttcctgttac
    11821 ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa atgaaattga
    11881 tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc tggattggca
    11941 aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga acgaccaata
    12001 catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat attttaaaag
    12061 gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac cctctttggt
    12121 ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc aaaggaggaa
    12181 aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt tttgtttatt
    12241 cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg agcagtatga
    12301 tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct cctgtgattt
    12361 tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc tttatctttt
    12421 atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga atcccgtttg
    12481 gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga taaaacggga
    12541 gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt
    12601 ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat
    12661 attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt
    12721 ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg
    12781 cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta
    12841 cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa
    12901 ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta
    12961 acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca
    13021 atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga
    13081 aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat
    13141 aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg
    13201 gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat
    13261 gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt
    13321 tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg
    13381 catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg
    13441 atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt
    13501 gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct
    13561 ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact
    13621 cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag
    13681 attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga
    13741 tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt
    13801 caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga
    13861 cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca
    13921 taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt
    13981 ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag
    14041 tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag
    14101 taattgggat attagcttca gttttcatca actgtgccac tgtatcagta taagattcag
    14161 gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat
    14221 tataacctag taatactccg tcacttccta tgatataggt gttgctgtca agaataccac
    14281 ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt
    14341 gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc
    14401 gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc
    14461 atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa
    14521 tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt
    14581 gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt
    14641 aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg
    14701 ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg
    14761 gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga
    14821 agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc
    14881 ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg
    14941 tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa
    15001 tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat
    15061 caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa
    15121 agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg
    15181 tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt
    15241 ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg
    15301 cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt
    15361 agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc
    15421 tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat
    15481 cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc
    15541 agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg
    15601 tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat
    15661 atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc
    15721 aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg
    15781 tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa
    15841 tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt
    15901 ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa
    15961 aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac
    16021 ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca
    16081 tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt
    16141 tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc
    16201 cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt
    16261 tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt
    16321 ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct
    16381 cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt
    16441 gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta
    16501 gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat
    16561 ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca
    16621 agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat
    16681 catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca
    16741 gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga
    16801 tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta
    16861 aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac
    16921 tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca
    16981 gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt
    17041 ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc
    17101 tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt
    17161 ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt
    17221 cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca
    17281 aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat
    17341 aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta
    17401 agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg
    17461 caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg
    17521 gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc
    17581 aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc
    17641 atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat
    17701 acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc
    17761 ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt
    17821 catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa
    17881 ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt
    17941 ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg
    18001 aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc
    18061 ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag
    18121 catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt
    18181 ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc
    18241 tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg
    18301 ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc
    18361 atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg
    18421 agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc
    18481 gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga
    18541 atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat
    18601 tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact
    18661 gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc
    18721 agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg
    18781 ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag
    18841 aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt
    18901 gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc
    18961 ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata
    19021 gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca
    19081 caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat
    19141 aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga
    19201 atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt
    19261 ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt
    19321 caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga
    19381 agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt
    19441 gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag
    19501 gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg
    19561 gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt
    19621 caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca
    19681 cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt
    19741 gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac
    19801 gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat
    19861 ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag
    19921 catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa
    19981 atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg
    20041 taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta
    20101 ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac
    20161 ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt
    20221 cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt
    20281 tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa
    20341 aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt
    20401 caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct
    20461 tacgccagag aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt
    20521 gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg
    20581 cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta
    20641 ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa
    20701 ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta
    20761 tagaaatata atggggttca taattgtttt caaaatcgat acccatttta cttttcggta
    20821 aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa
    20881 ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat
    20941 ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta
    21001 atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct
    21061 ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa
    21121 tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt
    21181 ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag
    21241 acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt
    21301 ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct
    21361 tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt
    21421 gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag
    21481 ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt
    21541 ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa
    21601 gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg
    21661 ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta
    21721 gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc
    21781 ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt
    21841 gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct
    21901 atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc
    21961 tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat
    22021 tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa
    22081 accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc
    22141 aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg
    22201 ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa
    22261 tttttctact gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat
    22321 tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata
    22381 aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc
    22441 atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac
    22501 ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa
    22561 catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc
    22621 cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc
    22681 tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt
    22741 tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat
    22801 catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa
    22861 tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat
    22921 cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat
    22981 ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt
    23041 catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta
    23101 aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct
    23161 tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg
    23221 cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg
    23281 cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta
    23341 atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg
    23401 ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca
    23461 ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat
    23521 atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga
    23581 tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt
    23641 tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga
    23701 aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt
    23761 tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg
    23821 aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg
    23881 ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat
    23941 tttctactga agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct
    24001 ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa
    24061 gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa
    24121 ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa
    24181 tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga
    24241 tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga
    24301 taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa
    24361 gcagtcttct cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt
    24421 catgacacat tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat
    24481 tttgaacact ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt
    24541 gtcgacctct gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc
    24601 tgcttatttt caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg
    24661 accgatctgg gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg
    24721 ttcaggaacg tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg
    24781 cttattttta tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat
    24841 tatgatccca gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga
    24901 tttcaaagta atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat
    24961 aatggttgtt tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt
    25021 ttgattttac gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct
    25081 caacagactg cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa
    25141 tagacaaatc ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta
    25201 tcttttgaaa ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat
    25261 tgtcccgtta gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat
    25321 ttcaggtttt tagttagata aggttcctta actactttcc cattgatctt caattgatca
    25381 ttttggtagc tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca
    25441 tctgctaatt gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca
    25501 accatatcac cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg
    25561 aataaaaatc cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca
    25621 agccagaagc gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt
    25681 tttttcattc tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa
    25741 taaaaataat tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa
    25801 aactatctct tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat
    25861 atccatggac ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct
    25921 agagctgtgc ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc
    25981 atcatgtgtg ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct
    26041 tcgttcctcc aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt
    26101 tcaggatgct ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct
    26161 ggttcgattt ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta
    26221 cttttttcag caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg
    26281 tcaccgcgat cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt
    26341 tctactaatt gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta
    26401 tgtccagtcg tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta
    26461 tatcctgaca tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag
    26521 aagcagcctc ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc
    26581 cgatgatttt ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt
    26641 atttgagttg tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc
    26701 atcatttact aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg
    26761 atccaaccta agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt
    26821 atagtttctc ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga
    26881 aagacttcta gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa
    26941 atagaaatcg ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc
    27001 caaataaaat ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat
    27061 gatttgattg actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc
    27121 cgtaagagct ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa
    27181 aaatacggtc gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt
    27241 tgttttcttt gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc
    27301 tccttctcta accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc
    27361 aattgataac ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg
    27421 atgccattta gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct
    27481 acttcctgat tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc
    27541 attttttttg tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca
    27601 gtgatctcag gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg
    27661 gttttattga gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc
    27721 agtggctttt taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc
    27781 aaatcattac cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt
    27841 tcttcttttg tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag
    27901 ttttctgttt caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga
    27961 acgaatcctc cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct
    28021 atgtagtgaa gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt
    28081 agtgggccag ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat
    28141 aatgggaaca aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctcttgta
    28201 ttcgattggt tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga
    28261 aaacaattat tttcctaatt ttataaagaa aaagagaata agctgcaagc ctttcaggct
    28321 gagggcttat cctcttttcg actaatctac ataatacatg attgcaaacg cgtctttacc
    28381 agtatgcgta gcgataaccg gattcgtatg cagtacaggg atatgcatgt ccgggaataa
    28441 ttcttgcaaa ccctctttga attcttgcgc caattctttt gcatccgcat gtgaaatacc
    28501 gatttgtcgc acatttggca attctttcag ttcatttttc aaattattaa accatttatc
    28561 gaacgttttc tttcctcgcc ccttagttac agggattaat tcagaatggg cgaaattcat
    28621 caccacgcgt atgttcaaaa cactagacag caaaccagtt gttcggctga ttcttccacc
    28681 ttttaccaag ttatctagtg tggatattcc gatgtataat tttgtattgt cttgtacttt
    28741 tttgacttct tcaaggattt tctctactgt tttaccttct tttgctaatt cagctgctcg
    28801 aattacctga aaagataatc cttggtcagt ggtatcactg tcgataaccg ttactttcgt
    28861 ttttgttaga ttactggctt gtcttgctgc ttctactgtt ccactcaagc cttttgtcat
    28921 atggatcgaa atcacttcac tgccgtcttc tcctagctca tcatacatcg agatgaattc
    28981 tccaattggt ggttggcttg ttttgggcag attttttgct ttcgccatca tttccataaa
    29041 tctttcgccg tctagttggt catcatcggg atatactgtt ccgtcgatca tgatggatag
    29101 cggcatgaca cgaatgccta aagcatcgcg tacatttttt tccatcgtac atgaagaatc
    29161 tgttacgatt tttacatttg tcattatttt ctctctctct ttctttaaca agatgcctta
    29221 ttcatgttaa actacatgta agacaatttc tatttttagt ataacagaca aataatgatt
    29281 tttcattaat tacacctagt aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa
    29341 cgtatgaaat attaaatgaa gtgtttaatg cagtcaccca tggaattggg actggattga
    29401 gtatcgcggg actagtggtt cttttagtaa aaggtgccca cttgcattcc tccctccata
    29461 ttgtctctta tgctatctat ggatcgatga tgattctttt atttttaacg tctactttgt
    29521 ttcattcatt gatttttaca aaagcaaaaa aggtgttcca agtattcgat catagttcga
    29581 tttttctttt gattgcagga agttatactc ctttttgttt gctgagtatc cgaggatggc
    29641 taggttggac tttatttgta ttaatctggt tgtttgcgat tcttggcatt atatataaag
    29701 cgttgacgtt gcataaacgg gagagagtat caaaagtatc tacgatcatc tatattttta
    29761 tggggtggct ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga atgactggtg
    29821 ttattttgat ggtagcaggc gggctttctt acacgatagg cgcattcttt tatagcttga
    29881 attctatccg ctatatgcat gttgtttggc atttgtttgt tatgttaggt gcaggtttta
    29941 tgtacttctc tgttttattc tttacttaat aaaaaaaata aagctatgga tgggacagct
    30001 ctaaataaca gctagtctat ccatagcttt tttatatcgt caattcgcct gtcccgcttt
    30061 ttacaggatt ctcattacgc tgatacgttt caaattgatg cggataacga tttttttcat
    30121 ccatttctcc ttgactagta ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat
    30181 aagcatctcc atcgaataca tgatggatga ctgtacggta taatatcgta caaaatggca
    30241 gaaattcttg ataaacagct gaccctccag cgatgaaaaa gataccgtct gtttcttttt
    30301 ccagagcaag cacgtcttcc acactatgca tgacatgcac gccttctgct ttataggaag
    30361 aatctctcgt caaaacaatc gtctgacgat tcggcaacgg acggctaccc atcccttcga
    30421 atgtttttcg tcccatcact agtgtatttg cttctgtcat ttgtttgaag aatttcagat
    30481 catttggcaa acgccaagga agacggtctt cttttccaat caatccgttt tcatcctgtg
    30541 cccaaattgc agctaacatt ttctcatcct ttctgattct aaacagcgat aggagctttg
    30601 atcgccggat gtgggtcata tccttctaat tggatatctt ccatctcaaa atcaaatact
    30661 gactgctttt cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt
    30721 tctttcattt gttcgatatg attgatataa agatgagcat ctcctaatgt gtgaacaaat
    30781 tcgccaaccg ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc ataacttgca
    30841 atgttgaacg gaacgcctag aaacacatcc ccactgcgtt gatacaattg gcagcttagc
    30901 cgtccctctt gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc catgctagga
    30961 acatcttccg gattccatgc agaaacgatc aatcttctag aatcaggagt ctttttgatt
    31021 gcttcgatca cttgtttgat ttgatcgata aagccaccgt cttttgtttc ccagtgacgc
    31081 cattgatagc cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga atcatcggtt
    31141 aggatcttct cgcaaaaaag cttgctctgc tttttatatt cttcattgaa ttcaggatct
    31201 gtcaaacatc tatggccaaa atcagtcata tctggtccat tatagtctgg acttttgatg
    31261 tagcgttcaa atgcccattc atcccaaata tggtttttat tttgtaatag atagcggata
    31321 ttcgtatctc cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt
    31381 ttcgttgtta acaaaggaaa ccctttttgc agatcaaacc gcatttggta tccgaataag
    31441 cttaatgtgc ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa caattttctt
    31501 cctaagtcaa gatacgcttg ttccatcctt tttcctccta atcttctacc cattcactca
    31561 agtattccca ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt
    31621 gcaattcttg taatcgagtg aaattatctc cttgatgatt catctcttct tggagcaatg
    31681 agattttttc ttcaagttct gcaatctcat cttcaatcgt ttcccattct ttttgttcca
    31741 tgtaagataa tttcttcttt tcagcaggtt ccttgctctg tatttttggt ttcgcttttt
    31801 cagcaggctg ctgggtctct ttttgcttca gtaaataact ggacatgttt ccgtaataaa
    31861 gttcttgttt tccgtttcct tggaatacta ataacttatc tgctaccttg tccaagaaat
    31921 aacggtcatg ggaaacagtg atcacagcac ctggaaaact tctaaaataa tcttccaaaa
    31981 tcgtcaatgt tgctatgtcc aaatcattgg tcggttcatc caataataaa acattaggtt
    32041 gctgaatcag cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa agtttgccta
    32101 tcagtgtccc atgcataaat cttggaaata aaaatcgttc aagtaattct gctactccga
    32161 tttgactgcc atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc
    32221 gtttatctgg tgccatttct tcattttctt gtgtataata cgcaagatga accgtttcac
    32281 cgatagacat tgtaccgcta tccaggggaa ttctgccagc tagaatatta agcaatgtcg
    32341 attttcccgc accattttta ccagtaatac ctagtctttc ccgagattga atcaataaat
    32401 ctaaatgttc tagcagtgtt tggtcattta tacggtaact gccatctttg atttccagta
    32461 cttttttccc taaacgttgt gtcgctaggt tcagctctaa atcatcttct tggtttactt
    32521 ggtacaagtt ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac
    32581 gtgcctgtac gccagccctc atccaagcta attcctgttt ataaagccgc ttgcgctttt
    32641 cttcttgttc tacttctaca cgatcacgtt ctgctttttc taagacgtaa gcttcataat
    32701 ttcctttata ctcgtataat tttccaaatg aaagttcgaa gattcggttt gctactctat
    32761 ctagaaaata acgatcgtgg gtaaccatca atagtgcgcc acggtattgt ttcaaatagt
    32821 tttccaacca ctcgatcgct tcataatcta aatggtttgt tggttcgtct agtaatagta
    32881 aatcgggttc atcaatcaac acttgcgcca gactaactcg ttttttctga ccacctgata
    32941 aagtactgat ttttttatct aattcactga ttcctaattt ttgcaaaata atctttgcgt
    33001 tggtatctgt agtccaagca tcttctttat tcatttgttc ttctgcttta gcataacgtt
    33061 tttgcacatc agggtcttct ccgttttccc ctagttcgat caatgcttcc tcatatgctc
    33121 gaacagtttg gatcaagggg ctgtttcctt gaaagactgc ttggagaaca gttaattcat
    33181 cggaaaattc agctgcttga gaaagataac caatacggta atcagttgga taaaacactg
    33241 tttgccgatc accgtcgctg ctatcgatac cggcaataat atttaagaga cttgttttac
    33301 ccgtcccgtt cgtcccaatc aacccgatac ggtctttttc gtggaccaaa aaagaaatct
    33361 ggtcaaacaa atctttttca ccataagttt ttttgaggtc agttactttt aattctttca
    33421 acatgttccc tccttatctt ggatattgta tcataaaaat agtgaacttc aggaaaattt
    33481 ttttggtatt tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc
    33541 tgatatgttt tgttttttat aaaacttgaa aatttttacc tttttatttt attggactcg
    33601 ttttttttga ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca
    33661 tcgtataaga ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga
    33721 ttttttcagc aacttcgtat tcatcaatga tttttctttc aagataataa cctcttagca
    33781 tttttttcgt aaacagacta tcttgctgga tcatccacga agaaatcaaa ttccccgagt
    33841 tcatttttat tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat
    33901 aaatatcagt caagttatct aaactttcaa taatcaactg agtatttttt tgatatgtct
    33961 ctctgatttc ttggatatct tcatcactaa taaatgtctg acgatcagca tatcttcttt
    34021 gccaaaacat tttcggatgg agaacgattc gcattaacct acggacaaca atgaaccaaa
    34081 agccgataaa agacaaaatc tggcttgtga tagaatgttc gaatcgagaa agaaaacggg
    34141 agtaaaaacg ataaccatta gaagaaagct tgcccgaacg ataactttca tccaatccat
    34201 cccgttcgat cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg
    34261 ttaaatcttc caagtacaga tcacgtatcc gttcttgata attttctatc actgcttcag
    34321 tggctaaaaa ttcttttgta ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa
    34381 cgacctcttc taaaatcatc aaagcattca aatccgtgct tttcgcttcc tctgtttccg
    34441 tcagcatagg caagacaatg atccctatga ccaaagtcac taagatgaca cccgccgtca
    34501 gaaacaaaag caaagaccgc tggtaaaaca tcatattatt gattgaaaat ggcaaaataa
    34561 aaatcgtagc caaactgact gttcccttta caccgccaaa tgttaagatc aagatctcgt
    34621 tcaactgttt tttgaacttt ttactgccat ctttgaatac ataaaaaaga ctgatagaaa
    34681 tgaatctgat cacaaaaagc atgacagaga ttaatacgat aatcgctaat aaaagaccgt
    34741 ttggatataa accgtctccc catactggcg agaacacttg cgtcaattcg attcctaaaa
    34801 atataaatac taatgcattc agtgtaaaag caatagttgt ccatgtactg tgggacaagc
    34861 tcgacaattc tgcatcaaac acagagattt tcctaaaacc agaagcttgg agaatacctg
    34921 ctgccactgc agcaatgatt ccagatacat caaatgcctc tgccaataca taggctagaa
    34981 aaggaagcaa aagttcgatc agcagatacc ctgtgacatc ttgtgctgat gctttttcga
    35041 tcaaatgaat tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa
    35101 tactagaaac tactagactc atccccgcgt ttactgctga aaaagatccg gtgatcagcg
    35161 caccaagagc aaactggaag gcagtaacac cagatgcatc attcaaaaga ccttctcctt
    35221 ccaaaatatg catcgccttt ttagggatat tcactcgtcc agaaagagaa ctgactgcta
    35281 ccgcatctgt cggacctaac gctgctccaa aagcaaagca ggcagcaagt gggacacttg
    35341 gcaacaggaa tgataacgtc gcacctaccc cgacaagggt caaaatgact cctccaaatg
    35401 ccaagaacaa aattgtcccg aaatttttga gaatcgaaga aatatctgct ttttctcctt
    35461 ctctaaataa caatggtgca ataatcataa ctaagaacaa ttcaggttca aaatcgattg
    35521 attgtcccca ctctgtcaga cctaatacca ctccaaggaa aatttggatc agtggagctg
    35581 gaatcattgg caaaatgcgg ctagctatgt tcgataaggt gatcgttact gcaaatacaa
    35641 taactaactc agcaaattcc atcgtccatc ccctttattt tttattttgt gtaagcattt
    35701 ttaaaaattg ttcttttttc tttaaaattt tggtttcgac tacttcaacc tgtgtttggt
    35761 tcccctctaa ttcacactgt acacgttttg actccaaacg aagcagatca tgtttcagac
    35821 gttccaactg acgatacatc aatatatttc ttttacgcaa cttacaaagt atttgacggc
    35881 ggtcatccat cgaaagggcg ccatagcgat catgcaacag ttttttttgt tgttcaaaac
    35941 tctctctcat tttccagatc tcctatacac aaacttccat cattatagca aagttctttt
    36001 tggatgaaaa atatttcatt gtttcatcgt ttattgctag ttagaaattt ccataaattt
    36061 ttcaacgcct cattctattc caaatagtat cagatatcaa gtaaaactta agaaagataa
    36121 aagtttctga caaaaggatg tttactttcc cattcctgcc tttcttgttc ttttatcaca
    36181 acaaaaaaat ggggctgcga caaaaatctt gtcacagtcc cttattctga ataaacggtg
    36241 tggaagagac agcctatcag tagtaataag acacatctct ttcacagcct catttttgcg
    36301 taaaaatcga tttattcgaa aaattccccg tatacatctt ctacacgttg atctggattg
    36361 atcacgatat acaagttacc tgttttttcg ctgatttttg ttgattgata ttgattgtct
    36421 aatccttctg ttcctttatc tagatatggg aagtacacaa gatcaggtgt gccatttcgg
    36481 taaaggattt ccattcgctc gatatcttcg tatttcaatg cgcgattaaa catgcccatt
    36541 tccagtccgc ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg
    36601 attttaaatc cagcacacgg atggatttca acaaattcac tgccattgat tcgtccaaaa
    36661 cttgttgtga tttgtttgat ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt
    36721 tctccatttc taaaataaaa ttttagcgct gtcatttctt tactcattaa aaagactcct
    36781 ttatgatgta atttattctt gtactacctc agtttagcac aaatttatgt aagtggtcac
    36841 atcttatgtt ttttcttcta atgacgtatc tgcccacttt aagtagccaa caaagctgag
    36901 cagatacagt ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt
    36961 tttttatcat tttttattct ttgttctacg acggcttttt cagctaattg caaagcttca
    37021 ctaacccact taccaggttt ttttccagtt tcttctagca agattttacc atctactgct
    37081 aattcgctta atgacttgat aggtaaacga tcataggatt ctcctacggc atccatctga
    37141 ggtgtttcgt caaaatagat catcaattct tctacttgca gtgcatgttt tttgcctaag
    37201 aagtacaatt cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt
    37261 gcttgaacgt ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc
    37321 tcattttctt ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc
    37381 aatgggacgg atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg
    37441 ggacagcaaa tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt
    37501 cccaagagca tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc
    37561 tcgtggtttt ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg
    37621 acaaaacgca acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg
    37681 cctacagctc gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt
    37741 tctccatctt ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct
    37801 gccaacgaac gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc
    37861 gtccgaaatg tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt
    37921 tcgatcccaa tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt
    37981 gttgcgatat ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct
    38041 acaaaataag cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg
    38101 tattccagag gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc
    38161 tgagataaag actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg
    38221 ccaggaccac ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga
    38281 ttgaattttt ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc
    38341 atgatcatcg taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca
    38401 gaagcctttt tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact
    38461 tcttcagtta atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg
    38521 atatattcat ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc
    38581 cctccaaaac tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa
    38641 tcgaatttcc atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat
    38701 aattgaaagc tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca
    38761 ttataggaac aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag
    38821 cagaaggggg agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag
    38881 cttatacgac tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta
    38941 aacgttcctt ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct
    39001 ggcaaagggt tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg
    39061 cgggtttagt tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg
    39121 gcagtgatat tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc
    39181 tattggcatt tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta
    39241 tgatgtatac gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg
    39301 gcggatattc tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac
    39361 tactgatgac cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg
    39421 gtaattcaaa aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga
    39481 ggatcgtcca cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg
    39541 aagggcaagg ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa
    39601 aaagacaaag ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag
    39661 tactatacta tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc
    39721 tgcaaaagga tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca
    39781 tcttctagtg cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt
    39841 tcttgaaaat atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa
    39901 gaaaatggat tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat
    39961 atacattcca catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct
    40021 gtgtccgtaa attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga
    40081 tcactgatct gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt
    40141 aaaatcgctt catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa
    40201 gatacatgat tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt
    40261 tttctcacct ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa
    40321 gtgttaaata ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga
    40381 aaaaggcata ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat
    40441 agtgtacagc tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt
    40501 ctgcataagg attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc
    40561 tttcatcatc caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta
    40621 cttctaaagc tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat
    40681 tttccgaggc caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta
    40741 acactgtttg tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat
    40801 ataacgattg ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct
    40861 gattttcttt caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt
    40921 cttctttcag tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta
    40981 aggattgtcc taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca
    41041 acaatgtttc ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag
    41101 ctagcgcaaa ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg
    41161 cttcactcac ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt
    41221 gcggatagta atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct
    41281 cattatcttc gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt
    41341 ggttgatcag ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt
    41401 cttcgcctag ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga
    41461 tttgtgcttg tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat
    41521 tcatttcaat tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat
    41581 aattcttttt cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt
    41641 atttttttgc ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc
    41701 tgttccagcc agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt
    41761 tggaaatcaa aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat
    41821 tttttcatac tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac
    41881 agtttaccat aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg
    41941 atcatcccgg caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga
    42001 ttttgcaaaa aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc
    42061 tgctttccgt ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga
    42121 ttgatggttt gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat
    42181 gctcggctta gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta
    42241 tgtttttgaa aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac
    42301 atggattgat tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa
    42361 tgattgcatt gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac
    42421 gggttgatct tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc
    42481 tgatcaatgg aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc
    42541 aacgcatctt ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt
    42601 ttaccattag taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttgcaa
    42661 ataaataaaa attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc
    42721 tctgtctctt atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta
    42781 gagccacaag cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt
    42841 ttgaatgcag gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga
    42901 cctttgcgag ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt
    42961 tcgccgttag ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca
    43021 tcttttttag ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc
    43081 atggatagat tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt
    43141 ctgagtggtc cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga
    43201 tatcatatga accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca
    43261 tagaaacgta acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa
    43321 atgcctattt tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt
    43381 ttctgatttg attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga
    43441 caaaaacgac aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc
    43501 gttttccttt atctgttggt gttggattga ttgctacagc atccataatg atatcattca
    43561 acacagccga agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca
    43621 gacgttccaa ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat
    43681 accggaattc atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta
    43741 caagatccca tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg
    43801 ctactcgctt gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat
    43861 cagaacggtc aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata
    43921 cttttccgcg tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag
    43981 aaacgaagtg ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac
    44041 gttcttctcc taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca
    44101 aactaaactt aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg
    44161 cttcatctaa aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc
    44221 ctagccctaa ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat
    44281 tgactgctaa aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg
    44341 cgtctgtcac gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg
    44401 cgatttctgc ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc
    44461 ctgtgtcgat cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac
    44521 gagttactcc aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa
    44581 tcgtagactt accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat
    44641 ttcctcctca caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc
    44701 atagattgtc aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa
    44761 aagaaaaact gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat
    44821 ttttcagaat catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg
    44881 tctcccatag taaatccagt attttcttct ggtaattcat aagactcaac attttttggt
    44941 tcttcttctg aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct
    45001 tctggatgga tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt
    45061 ggtgtcgcaa tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg
    45121 aacacttcaa caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg
    45181 ccaactggag cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac
    45241 aaggaaacac gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg
    45301 acagccaata catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact
    45361 agaccgtcaa ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt
    45421 ccactgagaa catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt
    45481 tcgttttcca ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg
    45541 atgattttaa agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct
    45601 tccaccattg atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg
    45661 acaacatttg taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct
    45721 tcccaaactt ttttagcatc taaacggcgt tttgaaagta gatagctgcc gttttcttta
    45781 tctttgccga tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg
    45841 ctgatatctt ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca
    45901 gccccttcga ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca
    45961 acatcgccaa cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc
    46021 tcttgatttt gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa
    46081 gtacggtact gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt
    46141 ttccttgaaa aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt
    46201 ttttcaaaac aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat
    46261 cgatacgaac agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat
    46321 ggtctctttc ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt
    46381 ggttctcttt gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca
    46441 cttctgcatt cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc
    46501 gttcccctat ttttcgttgg acagcaacta attcttctct gacttttcca tgtgcagaga
    46561 cttcagaaac agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg
    46621 ctatgaatac atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca
    46681 gcgaaactag cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta
    46741 ctgcgcgata catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct
    46801 tggcaacggt acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca
    46861 tgatataact cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc
    46921 ttaattcttg accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca
    46981 attgatcagt ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa
    47041 cagtcgttgt tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc
    47101 ttgaatcccc tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa
    47161 tactgctttc tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac
    47221 tagattctac tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta
    47281 ctactgatgt cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc
    47341 tgatccagaa ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata
    47401 gcgaaagaaa taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt
    47461 catcatctgt atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag
    47521 attgatgtct atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata
    47581 ccataggaaa aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt
    47641 tagtaaaatc gttccccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag
    47701 aagttctgct tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc
    47761 gccaaaataa gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat
    47821 ctgctgcagg cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc
    47881 tggtttttca gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc
    47941 ttgctctgtg gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg
    48001 attaaagaaa taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc
    48061 ttcctgatct ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa
    48121 atcataatgg accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt
    48181 agcaatgaga aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc
    48241 tgcatcaaga ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa
    48301 tcgttcgact tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat
    48361 gaagtgttca agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg
    48421 ccgattgact gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc
    48481 tcttgctact aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag
    48541 ctttcggatt tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac
    48601 gcaatgagcc tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag
    48661 agaaggatta gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg
    48721 gaggacgtat cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc
    48781 ccctcgtttt tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat
    48841 gatgaccaga ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc
    48901 tgtaggcaaa atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg
    48961 gccgggacga aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta
    49021 tcgcttcctt tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc
    49081 catctacatt ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac
    49141 gttcgattgt ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc
    49201 actcaataag atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt
    49261 caattgtctg accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt
    49321 gctgtaataa tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg
    49381 ctaacaaaag gtggatacat ctgctttgtt gcagataaac cgctgactca tcattgtttt
    49441 ttgccagctg ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa
    49501 aatctgctgc ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat
    49561 caatcaaaat ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat
    49621 aaaaaggact tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta
    49681 cttgaacagc aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa
    49741 aacgcaaacc agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta
    49801 gctttgcttc attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt
    49861 gataaaactt atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg
    49921 taaatccata aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac
    49981 taatccgcag cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct
    50041 ccttaagtta ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat
    50101 tgcatgcggt ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat
    50161 cgttttattt gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac
    50221 atatgtaaaa aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat
    50281 ataaatataa acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg
    50341 ggatctgttt ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct
    50401 atagttaatt gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt
    50461 tttcagccag ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc
    50521 tgcctctcca tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt
    50581 gattgataca tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg
    50641 attcaatact ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa
    50701 tgttcctttt ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga
    50761 atgattctta ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac
    50821 ataatcggaa tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc
    50881 tagatcaagt ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc
    50941 ccaaatacta taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga
    51001 ctttccatga ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc
    51061 ttttaccaac acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata
    51121 accggctcca ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat
    51181 tttcactttc tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta
    51241 taccttaaaa aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa
    51301 tatccatttg aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac
    51361 acagcttctg cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc
    51421 gcctggtgca taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc
    51481 atcgtacgag caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata
    51541 tcgcgatatg tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa
    51601 tcaaggaagt caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta
    51661 gctactaaat catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt
    51721 tgttctggtg tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct
    51781 tctgttttgc ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa
    51841 ttgattgggc caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc
    51901 actccttgtg ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct
    51961 tgttcttcga aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct
    52021 ccagagtaag gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac
    52081 tcgttcgttc ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa
    52141 ggaaattcag catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt
    52201 tctaaacaac gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg
    52261 aattccacgc cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct
    52321 ttgtcatctt gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt
    52381 tttgtttccc aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg
    52441 aataattcat ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat
    52501 gttctaaatc ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt
    52561 ttaccgaagg gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc
    52621 aattcgctca atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg
    52681 atattatcct cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc
    52741 ttctcaaata atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg
    52801 atgaaagaaa aaacactcac tgaatggagc tattgactta tccacgtgct tatcgatagc
    52861 cgtttatcgg tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga
    52921 acattgctgc tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac
    52981 cggtaaattt ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga
    53041 tagcagatgt tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta
    53101 ctaaatcctt accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag
    53161 cagctaagcc ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa
    53221 taatgatgaa tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg
    53281 tgtctgtgat taatccattg ttcataacca cttggatacc acgagcaaca gctactacga
    53341 ttgctacacc gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt
    53401 tcatgccata agtgattgct actacgatgg acattactaa gaataacata gtaatttctg
    53461 ggaagtacca gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa
    53521 ggtttgttaa ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc
    53581 aaggaatcaa actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt
    53641 tttgtttttt cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat
    53701 cttctttacg ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat
    53761 aacgatacac atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac
    53821 caataccttc acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat
    53881 ttaccgttga agctagacag cctacttgag accctactaa tacgattgct acagctacta
    53941 ctgtatcaaa gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt
    54001 cttctgccat tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa
    54061 gcattttttc ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc
    54121 ctgttttgtt gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga
    54181 ttgcagcagg tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg
    54241 gcgccatgaa aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag
    54301 cgacgatgtt gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta
    54361 cagcgattac tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt
    54421 tcttttttgt ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg
    54481 ctctaatgtt attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa
    54541 aacttttgtt caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag
    54601 ctttcttctt tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta
    54661 aaaggagaag aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa
    54721 atactcaaaa caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa
    54781 aaaaaagacc aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg
    54841 ttcgaactga tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa
    54901 gacagcactc acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata
    54961 gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag
    55021 aggcgtaata acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt
    55081 aatgacacct aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata
    55141 aatcgggaat gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc
    55201 cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc
    55261 ggccgctgga ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt
    55321 caaaaaacta aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc
    55381 agcaaacatc gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc
    55441 tgttaggact cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt
    55501 ttccagggaa aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc
    55561 tttatccaga ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa
    55621 taggtcgtgg actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta
    55681 tataattttt gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac
    55741 ttataaaaag taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc
    55801 agttcacggt aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt
    55861 tttagttttt gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt
    55921 ccttcgtgga tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt
    55981 tggaatttag ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca
    56041 agcatcgatt ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt
    56101 tttggatgtg tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa
    56161 gtatcgtaat ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc
    56221 gtaacaactt ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac
    56281 aataaatagt aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca
    56341 tgtttcccaa cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct
    56401 gcaaggatga attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc
    56461 tgcaatctct ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg
    56521 acacgtcttc ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt
    56581 tcaaaccaaa ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc
    56641 cttctactct acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg
    56701 aaacctgaac tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga
    56761 tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc
    56821 gcttaagtaa gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa
    56881 aaatatgcaa agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac
    56941 ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt
    57001 cctcgccaac aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct
    57061 cctactgctg gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc
    57121 ccttttttca tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt
    57181 gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata
    57241 aataccattc gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga
    57301 gaaaatttct tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact
    57361 tgtcctcgct tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt
    57421 gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat
    57481 ttacggtatt ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct
    57541 tcatcgtctg tgacgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat
    57601 gtagcagcca tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg
    57661 atataattca tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc
    57721 atttgctgaa tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata
    57781 ttgatctccg tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt
    57841 aaggctctgt gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat
    57901 ctaacatttg aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga
    57961 tgatcagctg atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac
    58021 cttctggcgt aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct
    58081 tcaacaaaga agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt
    58141 tacggttttc tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg
    58201 gaaaatgctg cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag
    58261 tcgtagagat atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt
    58321 ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc
    58381 cagcctctcg tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc
    58441 catgattatt gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt
    58501 cgtctaagta gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt
    58561 tatctccttt tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca
    58621 atccaatcaa ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat
    58681 ctcttatccc taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca
    58741 gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat
    58801 catgatcagt gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca
    58861 tccgagtaac agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga
    58921 ctaaaaagcg atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt
    58981 actgttccaa atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat
    59041 tcaagtagtg gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct
    59101 atcatatcag ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag
    59161 ccgtctagac actcagacgg actggtttct tttttctttt tcatagcatt cctgacagat
    59221 tccatggaac gttagccgat gatcttttac taaaaaatga taacgttctt ctactacttg
    59281 ctccacttca agtaaaagat cctcttctac ttcctcgatc gtgccgcagt tcaagcatag
    59341 taaatgatga tggaaatgtt tggcgccttc ttttctcaaa tcataacggg ctaatccatc
    59401 attaaaactt actttatcga caattttcaa ttctgtcaag atttcaaggg ttcgataaac
    59461 agtagcaagt ccaatttccg gactcttttt tttcactaaa aagaagattt cttctgcaga
    59521 taaatgctct ttctcatttt ccaaaagcac aagcagagta gcttctcttt gcggtgtcag
    59581 cttaaacccc gcttcatgta gctgctgttt tgtttttttc atcgccgaaa tagcattcat
    59641 gaccatttac tcaaccccaa caatttataa taattacaaa taaaaaaaga tataacacta
    59701 tgttaattaa aattattata agctagttaa gaaaaacatg caagggattc tcaattagat
    59761 tttttgacta gttttgtcac accgttttct tgtgtaatca atccttgttt caaaagatta
    59821 cctaatgcgc gcttaaatgc tcctttgctg ataccaaaag ctttcatgat ttcttctgga
    59881 tccgatttat ctgtaaagtt gatttgctga tctgcagaac gttctaaaat ggttaaaatc
    59941 atttgtgcat catctgatat agcttcatat gctcttggct tcaatgaaag attcagtaca
    60001 ccgtctggtc ggacaccaat cactcgacct tttatctgct cgcctagacg aggttcatta
    60061 tatcgttcag aaggatggat aaacccaaga tagaaatctt ccgttaaaac gtacgtacct
    60121 gttaatttca agcggtatgc tgttcctatc acatctttgt ttttcatttc ttctgttcca
    60181 tatttgctta aagatttgaa tattttttca tctgctaaac tgccccaaat acggtctttt
    60241 gcatcaactc taagggaaat catcaagcga tccccttttt taggccatag ctctgtcatt
    60301 gtcggcaatt catcaagaga tacgacaaag tctttatctg gaagaccgat atcgacaaac
    60361 gcacctaaat cttttcgtgt agcagtaacg attccaaaag cgtaatgacc gatacggctt
    60421 ttaggaattt gtgtagtgat ccgattttca tgtttttgat tttggtaacc gaatccttca
    60481 actgcttcac cgattttgtg ttcaccttct gatttgctta agccaaaggt ttgtccgttt
    60541 ttttggacaa agtagttttt ttcattttca tcaataataa tacctgtaaa tatagttgct
    60601 aataaatcgt tcattttttt ctccctctat agtgaaaggt tttcttaata catttctttg
    60661 ctatcataac ataatgttat ctacttgaat agctgagcct tggtttggaa aggtgaaaaa
    60721 aggagtatga caacagttgt gtcatactcc cctttttcga ataaacggtg ttaaacggca
    60781 ggctcacagc ctctttcatt tgcctaaaat taattgctcc catttcctcc gaaccatcca
    60841 gttttacagt tgatggttac gatgtaaaat ggcaaagcac tttctccaat gcctttcatc
    60901 aatgcagatt ttgttgaagt ggcatataga ttataatagc cttccccatt ttcgattctg
    60961 gctttttcta atctgtaacc tccttcaacg atatatccac gttggataca tgtagcaatc
    61021 actttgcttt ggacacctgg tgcccacgtc catgctggat tagatgcatc ggcaatatca
    61081 cttgctctgg cagatttgat tggctgattc gctgagtttg aagaggaagg ggtcgaggat
    61141 gccgcttctt gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct
    61201 gctgcttgag cggcttgttt tgcttgttct tcagctgctt ttttcgcttc ttctgctttt
    61261 ttcttttcct cttcttcttt tttctcttta tcagtgagca tcttatctac ttgtttcaat
    61321 tgttcattca actgagcaga tagatctgta tttttcactt tttctactgc ttcttttgcc
    61381 gtcttgtatt ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa gactatagct
    61441 gttttttctt tagctgtttg caattcagca tattgatttt tagcttctgt ttgtgcttgt
    61501 tgaatcaatt ttccaaaatc tgtgttatcc gtttcgattg cttggatctc tttatctgct
    61561 ttcaacttga tgtcatctgc tagatgatca tcgacgatgg caggtgctat gaaccactca
    61621 ttcacttttt ggatatctcg gatcttagat tggatatctt ggtaagttgt ttctaactct
    61681 gaatatcctt tttcattctt aagactggat aaatcatttt ttagttttga taaatcttga
    61741 tgaatcatat ctgcacgaat aaattgatgg tgatcatctg tataaaaggc agcaagtttc
    61801 tcatgaagtt cttccatttg ttgctgtctt tttgcttctg ctgctacttc ggcttctact
    61861 ttttgatgat ggtcataata agcccagctt cctccaccga gtagaagtac tgctgctaaa
    61921 acgagataag gtactttttt tgattttttc ttttgcgctt tatctggatc tgttttttct
    61981 aagtttgatt cttgatgtgt tttttcagta tctggcacga ttgttattga tttcgcttct
    62041 gcttcaaaca gcttaggctg gttctcttct ttttctattt ttgcttcaag atcagtaccc
    62101 tctttgctta cagattgatt ttcagcatcg gattcactat ttatttcatc gggctctgtt
    62161 gactttttgt ctgtgttttc gagtatttct tttttctctg cttccggcag tttattcgtt
    62221 ttttcctttt ctaattgttt ttcagtaaat acttcagttt tttctgtgat atccgttttt
    62281 acgtctttta tttttactgt ttctttacct gtggcgtttt ctctatcaga gtctttttca
    62341 gtttcttgtt ccttttctgg ctctactttg ttttcctgtg caattttttc actgatcatt
    62401 ttgagcagct ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt gatataagcg
    62461 gatagtatcg aattttcttc ctcattatcg acgatttctt tcagttcagc tactcttctt
    62521 ttttgttttg attcaagcgt ttcttcttga ttctttcctg attcttccga agtctcatga
    62581 acagttgctt tcttctgttc atttttctct atattagtta cttgatcgtg agcaggtggt
    62641 tcttcgtctt ctggttctgc tttttcgaat aattccatta cagattctag aggtaaatct
    62701 ttcaattccg accattcgat tggatcgttt aattctgtat caggaaagac tgcatcttca
    62761 gttgatttat cctgcatagc tgcatctgta tttgtttctt caattgattc ttttatttgc
    62821 tgatcttctg cagttatttg atgaccgcag tctggacaga tagtattctg ctccattttt
    62881 ttcgatccgc aatttggaca ttttttcatc acttagcgat cggctccttt actaggttta
    62941 cgctaccatt taatctatca tattctatac gtcacgccaa tagcttattt ttaggattta
    63001 cttaaaaata atgaatgaat ttccttccta tagaaaaaaa agcttgagac tgcgtagtct
    63061 caagcttttt ttcttttaaa ataaaccaaa cagttttact gatttctctt ctggtcaata
    63121 gtccacgctt attctaagaa atcttttaat tgttttgaac gagaaggatg gcgcaatttt
    63181 cttaatgctt tagcctcgat ctgacgaata cgttcccgag taacaccaaa tactttccct
    63241 acttcttcca atgttctcgt ccggccatca tctaaaccaa aacgtaagcg aaggacattc
    63301 tcttcccgat cagtcaatgt atctagtaca tcttccaatt gttcttttag taactcataa
    63361 gctgcgtgtt ctgctggact tgttgcttcc tggtcttcta tgaaatcacc taagtgagag
    63421 tcatcttctt caccgattgg cgtttcaagg gaaacaggtt cttgcgcgat tttcaagatt
    63481 tctcggactt tttctgttgg aagatccatt tccgcaccga tttcttctgg cgtaggttct
    63541 cttccaaggt cttgcagcaa ttgacgttgg atacgtatca atttattaat tgtttcaacc
    63601 atatgaactg gaatacggat cgtacgtgct tggtcagcga ttgctcgagt gatggcttga
    63661 cgaatccacc atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca
    63721 actgccttca tcagtcccat gtttccttct tggatcaagt caaggaactg catgcctcgt
    63781 cctacataac gtttagcgat acttactact aaacgaaggt ttgcttctgc cagacgctgt
    63841 ttcgcttctt ggtcgccttc ttcgattttc aatgctaacg ctacttcttc ttccgcagtt
    63901 aaaagggaga cacggccaat ttctttcaga tacatacgaa ccgggtcatt gatttttaca
    63961 ccggttggcg cagaaagatc ttctgttttt gcttgttcag cttttttctc agcactcttc
    64021 aagctgtgaa ttgatggatc accattttca tccaccacac tgatacctgc gtcttcaatc
    64081 ttttggatca atttgtccat ttcatctgca ttcaaagtga atggtgtagc aagctgattg
    64141 gataaatcat catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact
    64201 gctgcttcat acgttttttt atctgttcca tttgccataa aagaaggccc cccttgagta
    64261 gctatttggc ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc aattcaagct
    64321 caagctgttt gtttccagtt cgtctggctt cttgctgcat ttgttttttt aatgttattt
    64381 catcagctaa gccagatttg ctgatcacat ctagcaagtc ttggatttct cgtggcgaac
    64441 tttcttctgc caccggtaaa tattcgattt ccaccaccaa ccgccttaaa ttttcatctt
    64501 gcaaaaagtc taaaaactct gcaaggttga attcataatg ttgtgagaca tatgcatcga
    64561 ataaaaaata aacttctgca tatgcatcat gaataaactg aaattctcgt tcttttaaaa
    64621 tattacggac agaaacttct ttaaatgcac gatataatag aatctgctct gccttttcta
    64681 cttgcgtcaa aggtctcttt ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac
    64741 tttcctgagg ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc agttgttttt
    64801 gtatcgtctc tctgccgatc tgaaactctg ttgacagttg agatagatac atatcttgtt
    64861 cgattggtga aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt
    64921 ctttttcatt atctaaattt ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg
    64981 tttcgcgccc atgagccact aattcttgga aagatgcttc tccatactta cggagatatt
    65041 catcaggatc aagcttttct ggaacgacta cgacagaaag tgctaatcga ctgttttgcc
    65101 gaagcaattc aatccctcga ttggttgctt caaaacctgc gttgtctccg tcatagcaaa
    65161 agaccaattc tttcgtcagc ttttccattt gttggatctg ttgagctgtt agacttgtcc
    65221 ccatagaagc taatccagtt ttgataccag cctgccatgc agcaatcaca tccataaacc
    65281 cttcgaacaa caaaatttct ccgtttttcc ggatatcgct tcttgcttta tcaagattaa
    65341 ataaaacttg tcttttattg aacagttctg tttctggact attcaagtat ttgggctgat
    65401 ctttttcctg ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt
    65461 gagaattacg aagtggaaac atcactcttt gataaaaacg atcagacata cggccatcat
    65521 catgcgtgac aaacaatcca gagcgttcta acaattcatt tgaaattgat tcattcttga
    65581 atacttgctg aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat tcttcaatta
    65641 attctaaaga caacccacga ctttgcaaat attctaaagc tggctgtcca gcttttgtat
    65701 gcaatagcat gtgatgatag atttccgctg ctttttcatg agcagcaatc agtttccccg
    65761 tagcagattc aggatttcgg acagctatag tacgctgacg ccattgatct tctaacggta
    65821 tctgttcgaa gtctgccact tttaccactg cttctggaaa acttagacct tctagttctt
    65881 gaagaaaagt aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt
    65941 cttctgcaac agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat
    66001 ttttaccaga ctttttcaac tggacatatt gtccaatcac ctcaacgata ttcgtttgac
    66061 tgcgtactgt ttcgataacc tcttgaggaa tccgttgagc cattttttca ccccctgctc
    66121 tattgatatc ttttgggttt aacagaagat atttcaggag aataaccggt aaaaaatcgc
    66181 actaactgaa atcagtgcga ttttaaagga cacaattata catgtttgat tgtatcatat
    66241 ttcattgctt tttcaagtat aacacatttc tatctcatta agcatcaatt tgctactctt
    66301 ttcagtaact gattttgaat atcagccttc cagtacaata ctcgaagaac aatatttacg
    66361 agcatgaata caaagaagta tcctacgctc tctcccaacc ctaagtttac ttccaaaatt
    66421 ccatggaaca gcattgcagc tacatatatc cctacgatcg tcgttactgc taggaagatg
    66481 cgcaatgggt tgaagggata acaagctttt acaactgcca tgcaactgat cccaatcagc
    66541 aagtaataca tcaaagtagt agtttcagca tttccccatg cgaatgtttt tccaagtaga
    66601 tacaccgcaa tgatattcac tactacaagc aaggcatttg gtaaagcatt aataagtgct
    66661 gtaggcaaat aacggtaagt gatttttctt ttatcttgtt caaaagataa aaagaaggaa
    66721 ggataccctt caatcgctaa atcaatcagc gtgatttgga tagggataaa cggaaatcca
    66781 attgctgtaa cggcacatat aatcgataaa ataaatgaat aaatcgtttt gataaaaaag
    66841 atgcctgaga ctttcgtcac attgttgacc actcggcgtc cttcaaacag gacttctggc
    66901 aatgtagtaa aatcagagtc caacaatacc agattcgcaa tctgacgtgt tgctccatca
    66961 ccttctgcca tagcaatgct gcaatctgct tctcttaacg ccaaaacatc attcacaccg
    67021 tcgcctgtca ttgcaaccgt tctgccgctt tctttcaatt catttaccag caatttcttt
    67081 tgctgcggac tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg cacttccatt
    67141 tcttcagtaa tttgagaaag atcgacatac gcatcataat tcggtaatcc tgcacgtcta
    67201 gcgatattgg agacagtaaa aggattgtct ccagaaatca ctttcagatc cacaccttcc
    67261 tcatgaagat aagctagtgt ttcttgagcg ttttttcgga ttggatcatc gatttcaaac
    67321 agagcaaccg gctcaacaaa tgctggtttt tcttcgttca attgctgatt tggagcgatc
    67381 cccaacataa gtacgcgata gccattcgct tgggctgttt ttagactgtc cggaagccga
    67441 tcttcttcca ccaaacgttc aggcgctcct acgtacacga ctcccagttc tggaaattcc
    67501 atcgcgcccc atttgcgatc agaagaaaat gcgactttgt ttgtcacatc ataccgatta
    67561 gaaagctcaa aataatctct catagcccgc atagtgatgt tgttatcact gctttctgcc
    67621 agatacgtcc ccataatttc tgggatggct ttctcgtaca gcggatgtag tgcttctacc
    67681 cgctggatac tcattttccc ttcagtaatg gtccctgttt tatccaagca taatgtatcg
    67741 acatgggcca gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt ttttgccagt
    67801 ttggttaccc ctgtcgttaa tgcaatactt attagcaata ccaaaccctt tggaagcatg
    67861 cctaacaaag ctgctgcaga tgcaataaca gaaattttga tagaagcatc gcggatgaac
    67921 agtgcttcca aaaatagtat gatccccaaa ggaataatca cgaaactagt gaatttggat
    67981 actttgcgaa tcgaatctac aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta
    68041 gtcaagcgga cagcataatt atctgcacct actcggataa cttcagccat gacagaaccg
    68101 cttgtcaaaa agcttccaga aagcaattca gctccagttt ctttgacaat caaatcagat
    68161 tcgcctgtca aaagagcttc atttgcttct gcccgtccag atataacctg taaatcggct
    68221 ggtatctgtt ctcctgctcc gatttttacc aaatctccta ataccagctc tttagtagaa
    68281 atcgattgtt ctttaccgtc ccggatcact agtacttctg attgggaaac aatcgataat
    68341 ttccgtacaa gatttcttgc atgtatttct tgataaattc caataacgat attgacgaca
    68401 ataattgcta aatacgccat gttggaatac gcacctacaa ataacagaca aatcccaata
    68461 ccaaagttca gaaaattgaa taacgtcaat atattatcgc tgaatatttg ttttgtactt
    68521 tttgatgcat cttcctggta gtcattttgc tggccagaag ccattttctg aactacctct
    68581 tgctgtgtaa gtccattcat tttctttttc tccattttcc gccaactttc aaaaataatc
    68641 acgtgtttaa gtctagcgtg ttccaagatt aaaaacaact tgtttttata aaatttatag
    68701 aaaaacacac gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt
    68761 gctgttttta ttatcaatct atctttactt atattgttcc tccaaacggc tcatccacca
    68821 acctagtgct gtaccggcta taaaaagaac aggacaagct agtaccccag caactcccaa
    68881 tcccgcgtaa tcggtaggaa tgatcataat cgttgaagca aaaactacac ctaaaatgaa
    68941 atggaatagc ttgctatatg cctttgagaa aatgtaatcc atgatttttg acaatccaag
    69001 aacacaaaga atcccaccaa ttccgatcgg cacaatcact tcaagtcgta cttctttaaa
    69061 tccgtcagcc atcgctttat acaatcccat atacaccaaa aaattagaag ggctcaatcc
    69121 tgggacgatc atacccaaac caatcaatcc gccggcaatc agccatgttc caaaattctg
    69181 atcgacttgc ccgtcaaata attgagcgcc gtataataaa aacaaataag ccagaacaaa
    69241 agtaacaacc atgatgatca catcagaagt atctcttcct tttttccctg cttctttcca
    69301 taacgcagga acagttccag cgatacatcc gacaaaaaac cataatatcg ttgtttcata
    69361 cgcacctaat aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag
    69421 accaacaggc agaaaaaaca aaacattttc tttgaaattt tttgtaaggt gggctaaaaa
    69481 agaaatcagc cgctcataca tgccaaagac agctgctaat gcaccaccgc tgactcctgg
    69541 caagataaat cctgaaccga taaacattcc cttcacaaac cgtaacatcc agtctttggt
    69601 tgtgtacccc acttcttgat tttccatttt ttgccttttt tccactattt gcgtactcct
    69661 tctttcattt aattttattc atgattgaac atttcctgct attttactta aagacttttt
    69721 cataggtctc tattattttt tcattcattt tgacaatatc aggataaaac atgttacctc
    69781 cattatgata gagatagccg ccattgtaaa gcaaggactg gatccgccaa tatcggtatt
    69841 gtttcccttc ctcatttcct aaaagtgggc tcagcacatc tttagaatac gcttctgcta
    69901 attctactgt gttttctccg ccatgatttt ttacataggc aatataatct ttcccaaaat
    69961 tatatgcttg gacagctgtc cataaatcgc atccttgttt ctttgcttct actatcattt
    70021 ccgccaaata tttgacacct tgatcaatac tgtcagctgg attatcaatg atctcagctt
    70081 ctccatgcag actttccgag ctctgcatga gatctgtccc ttttcctttt gattcggtca
    70141 atattatcgc cttgatcaaa ttctcgtagt tttccatatg ataatttttt gcagctgttg
    70201 ttatttctat ttgatagctt tccactcgtt ttaccgaacg ataagttctt cctcataaaa
    70261 acaacaccgc accaataacc gtcagaatga tcatgtaaaa aagaatacgg ataatagata
    70321 atcgttttcg cttcatttca tcacctagtt attttgacat ctttttttat cttactattt
    70381 ctttatcttc ttggctattt cttccttgat ttctgtgtac gccttaagaa aaaatgaatt
    70441 ttcctgtatt ttttgacatc ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc
    70501 caatgactga tataatcgtt atactttaca cagtaggtga ttatgtgaaa aaagaaaaga
    70561 aatatgcaaa aatgccagat gggagcgaaa tttactatga aaagagcggt caaggcttcc
    70621 ctctcttttt actgcatggc aatgacggca gcggccgctt tttttcagaa caggtccctg
    70681 tactcgaacg ctactatacc gtgtatctag tagatagccg aggacatggt cgttcgacaa
    70741 acgaagcatc tatgttgaat tttcagttaa tggccgagga tctgaatacg atcatgttgc
    70801 tggaaaaaat cgatcaggca gattttcttg gatttagtga tggagcaaat ctagcactgg
    70861 tatttgccag cagcttccct aaaaaagttc atcgattgat tttaaattct ggaaacacgc
    70921 ttgttaaagg cgttcgtttt tctgccagag tcatcagtaa tattcattat gcatgggtat
    70981 ggttactctc tttatttcgc ccaagtcttc gcaaaaatct tttagttatc aaacttttgc
    71041 tgcatgacat cgggcttaca gaaaatgatt taaaaaaaat caactccccc actttgatca
    71101 tcgtgggaaa aaaagatgta atcaagctaa aacattcttt gtatatagct aaaactatac
    71161 caaaagcttc ctttgtgctt gtgaaagaac aaggacatga attagctaga aaagatcctg
    71221 aacgcttcaa tcgagaagtt cttcaatttc tttctgaaac ataactatta ggaatagaag
    71281 gtgatttatt tgttaaaaaa taccatacaa tggctgaaaa accacctagg actgttcaaa
    71341 acaatctttt tgatttctgt cattgtcatt attgtcggtg aattgatgtc catcggtaaa
    71401 actctatcta tccagcaatt aggagaaacg tttgccacta tacctgtatg gaaaagcgga
    71461 ctaatgctat tgattggtct tatctctgtg ttacctatgg ttggttatga tattattttg
    71521 aatcggatgc ttgaacaaaa gcagaatcct cgttacctgt ttgagacaag ctggctgatc
    71581 aatacgatca acaatattgc cggtttcggc gggtttgtta gtgtggggct tcgctctgaa
    71641 ctatatggac aaaagaaaga gagcagaaac gtgatccaag cattatcgaa agttttcttg
    71701 tttttgatgg ctggactttc tatttacagt ctgctttctt ttttacttgt agtcttcacg
    71761 ccagttgctc cttttttgaa gcaatattgg atatggttga tcggcggcgg tctatatttt
    71821 cctgtcgtct ttttatttac tactttcaaa aagaaaggat tccttggtgg tacctcactt
    71881 aaaactcaag gacagctatt gctagtttca ttgttggaat ggaccggtgt cctgtttagt
    71941 ttcctttctg ttggttattt aatggaaatc cggattgatc tgtggcaaac aattccatta
    72001 ttcattgctg cttcagtgat aggaatcgtg tctatgattc caggagaaat tgggagtttc
    72061 gatgtaatga tgattatcgg gttgtccgct attggtgtgc caagagaaat agtcgttgtg
    72121 tggatcttgc tctatcgcct gttctactac atcattccgt ttttgattgg aatcgtcttt
    72181 ttcttcaaaa atatcggttc cacttttgat cagcgttatt ctggtatccc taaacaatta
    72241 gcaacagaaa ttgcgcataa aatcgtggtt gtcttattat acttttctgg aatcatgcta
    72301 gtactttctg ccactatccc gcaagctttt acagagtttc gctggcttca tagtttgaac
    72361 ccattgaaat ttcattttat tatccaattt cctagtatac tattgggatt tcttttaatc
    72421 attatgggca gagggatcgc agcacgtgtg aaacgagcat atttgcctac aatcttcttg
    72481 attgttctag ctttacttta tgttttatta agtgatttta gtttcacacc ggtggttttc
    72541 ttgtctattc tgcttctgat cattcttgct tctaaaaacg aattatttcg ggaacaattg
    72601 atctattcat gggagtggcg tacgatcgat ggaatcatta ttggtgcact gtccttgctt
    72661 tacatcgtta ttggggtata caatctgccg gatttccctc atcggcgtca ccattttatt
    72721 tcctttttct tatttccttc tgaaaagatt tggttttcgg gattgttggc catcatcgct
    72781 gttagtttca tgatcgttct atttgttcat ttccttcaag gagaaaagaa acagattggc
    72841 gaagccttta atgaagaaaa ggccctgaag atccttacaa cttatggagg aaactccgat
    72901 agtcaattga tcttcttaaa agataagcgg atgtttgcct atgaaaaaga cggtgaacca
    72961 actgttcttc tccaattcgc ttgcttcaat aataaatgta ttgtaatggg tgatccttct
    73021 ggtaagaaag aagattttcc agaagcaatc gaagctttta tcgaagaaac agatcgacta
    73081 tgctatttac cagttttcta cgaaacaagc gaagagattg ttatgatttt gcatgaattc
    73141 ggctacgatt tcattaagat gggggaagaa gcttatgtag acctgaatag ttttacaact
    73201 tcagggaaaa aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg agaaggattt
    73261 acatttgatg tcttacaacc accattttct actgagcaaa tgagtacttt caaaaatatc
    73321 tctgataatt ggttgggttc acgaaaggaa aaagggtttt ctttaggctt tttctctgaa
    73381 gattatctac aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga aattgtcgct
    73441 tttgcaacga tcatgcctac ttacacgaac aataaagtag gaacgattga tctgatgcga
    73501 tatgattcta aaaaagcacc ttctggcagt atggatttct tgttcttgca tttgtttgcc
    73561 tatatgaaag aagaacatat ccaatggttc aatttaggga tggcccctct ttcaaatgtc
    73621 ggaacttcca gaaagagttt tttacaggaa aggattgcat acttggttta cgagtttggt
    73681 tctcattttt attctttcca tggattaaga gaatataaaa gcaagtacgc tacaaactgg
    73741 gtctcacgtt atactctata ttctagagat agttggattg catatgttat gattgctttg
    73801 ctgattattg ataatgcgcc agttgagcag acacaaggta gaataagtgg tttgaaaaaa
    73861 tggatacgaa gaaagtatta aaaactgcat accgatagat gaacactctc atgatgaata
    73921 aaagagactg aagtaaaacg aaagttcatg cttctacaat ctcttcttat acaattgaag
    73981 aacaaagagc ggtccaaaca gaaatcctgt ttggaccgct cttttatttt aaacattttt
    74041 cattaccaaa atattcgagt cgcgtttctt atgcttcgat ctctgtaacg atacctgaac
    74101 ctactgtccg tccgccttca cgaattgaga atgtcgttcc gttttcaaca gcgattggat
    74161 ggatcaagtc aacatcaatc gtcacgttgt caccaggcat gaccatttct gtatcttctg
    74221 gcaatgtgat tgttcctgta acatcagttg tgcggaagta gaactgtgga cgatagttgt
    74281 tgaagaatgg agtatgacgt ccaccttctt cttttgtcaa cacgtacact tctgctttga
    74341 attttgtatg tggagtaatg gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt
    74401 cacgttggat cccccgtaac aacacgccta cgttatcccc agcttccccg taatctaacg
    74461 ttttacggaa catttctaca cctgttacaa ctgctttttg tgtttcaggt ttgatcccta
    74521 cgatctctac ctcatcaccg acacgaacag caccacgatc aatacgacct gaggcaacag
    74581 ttcctcgacc tgtaattgag aagacgtctt ccactggcaa tagtaatggt ttatctgtat
    74641 cacgttctgg tgttgggata tattcatcta ctgtatccat caattccata atagcagctt
    74701 cagcatctgg atcgccttgc aatgctttta atgcggaacc tttgatcaca ggagtgtcat
    74761 cgcctgggaa accatattcg ctcaataact cgcgaacttc catttctacc aaatcgatca
    74821 attcttcatc atcgactaaa tcaactttgt tcaagaaaac aatcaggtat tttacaccaa
    74881 cttggcgtga caataaaatg tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa
    74941 caactaagat tgcaccatcc atttgtgctg cccctgtaat catgtttttc acatagtccg
    75001 cgtgtcctgg tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg
    75061 cggtattgat cgtaatccca cgttcacgtt cttctggagc tgcatcaata ctagcatagt
    75121 cttgaggatt tgctaggcct tttttgccta atactgtagt gatcgcagca gttaaagtag
    75181 ttttcccatg gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta cttctgtcat
    75241 aatgttcttt tgccataatt tacaaacctc ctaaataata tagatcactg aaaaatttca
    75301 gctctcacct ttttattata gtcttttttt gactaaatca aaaagaatac gtccgactta
    75361 ctttttgtaa gattcgaact aagcttatta tacgtcaata ttggtcaaag tcaactaaga
    75421 gcactcgtct gtcttatacg cgaaaaaagt accttttata aataaaaaaa tgtgtttttt
    75481 gagaaaaact tcaagatagt ccttaacttt cttttgaagc aataagaaca aaatgaggaa
    75541 aagtttaaaa ttcagctgtt gccaacaact ttcaatagac gaaatcttct atttcgttaa
    75601 taataaggaa agaggtgaag tacatgaaaa aattaattgg cgtacttgtc gttttaggca
    75661 tatttctcgg cggaagtttt gcagcttatc attattttta cggcggcgaa gcttattata
    75721 caaaaatcac gacgaatggc gaaatgtcag ctacccaagc agataacgga gagaaattca
    75781 cgacttatac ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc
    75841 ttcgcgaaga aagagaacat ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc
    75901 ctcgtaaagg tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg
    75961 cagaaaaaat cgataattag taaatggtct taaaatttta ttagataaaa
//
LOCUS       DAZKMZ010000004        74165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000004 DAZKMZ010000000
VERSION     DAZKMZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.004"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..522)
                     /gene="mreD"
                     /locus_tag="ACG8M5_000343"
     CDS             complement(13..522)
                     /gene="mreD"
                     /locus_tag="ACG8M5_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0357627.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(526..1380)
                     /gene="mreC"
                     /locus_tag="ACG8M5_000344"
     CDS             complement(526..1380)
                     /gene="mreC"
                     /locus_tag="ACG8M5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0357628.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(1535..2173)
                     /locus_tag="ACG8M5_000345"
     CDS             complement(1535..2173)
                     /locus_tag="ACG8M5_000345"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0357629.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(2174..2824)
                     /gene="rpe"
                     /locus_tag="ACG8M5_000346"
     CDS             complement(2174..2824)
                     /gene="rpe"
                     /locus_tag="ACG8M5_000346"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0357630.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(2837..3736)
                     /gene="rsgA"
                     /locus_tag="ACG8M5_000347"
     CDS             complement(2837..3736)
                     /gene="rsgA"
                     /locus_tag="ACG8M5_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0357631.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(3901..5970)
                     /gene="pknB"
                     /locus_tag="ACG8M5_000348"
     CDS             complement(3901..5970)
                     /gene="pknB"
                     /locus_tag="ACG8M5_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0357632.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(5967..6707)
                     /locus_tag="ACG8M5_000349"
     CDS             complement(5967..6707)
                     /locus_tag="ACG8M5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0357633.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(6720..8078)
                     /gene="rsmB"
                     /locus_tag="ACG8M5_000350"
     CDS             complement(6720..8078)
                     /gene="rsmB"
                     /locus_tag="ACG8M5_000350"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0357634.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(8078..9016)
                     /gene="fmt"
                     /locus_tag="ACG8M5_000351"
     CDS             complement(8078..9016)
                     /gene="fmt"
                     /locus_tag="ACG8M5_000351"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0357635.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(9009..9500)
                     /gene="def"
                     /locus_tag="ACG8M5_000352"
     CDS             complement(9009..9500)
                     /gene="def"
                     /locus_tag="ACG8M5_000352"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0357636.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(9515..11923)
                     /gene="priA"
                     /locus_tag="ACG8M5_000353"
     CDS             complement(9515..11923)
                     /gene="priA"
                     /locus_tag="ACG8M5_000353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0357637.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(12176..12481)
                     /gene="rpoZ"
                     /locus_tag="ACG8M5_000354"
     CDS             complement(12176..12481)
                     /gene="rpoZ"
                     /locus_tag="ACG8M5_000354"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0357638.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(12486..13100)
                     /gene="gmk"
                     /locus_tag="ACG8M5_000355"
     CDS             complement(12486..13100)
                     /gene="gmk"
                     /locus_tag="ACG8M5_000355"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0357639.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(13587..13871)
                     /locus_tag="ACG8M5_000356"
     CDS             complement(13587..13871)
                     /locus_tag="ACG8M5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357640.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(13889..14779)
                     /locus_tag="ACG8M5_000357"
     CDS             complement(13889..14779)
                     /locus_tag="ACG8M5_000357"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0357641.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(14799..15017)
                     /locus_tag="ACG8M5_000358"
     CDS             complement(14799..15017)
                     /locus_tag="ACG8M5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0357642.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(14987..16687)
                     /locus_tag="ACG8M5_000359"
     CDS             complement(14987..16687)
                     /locus_tag="ACG8M5_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0357643.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16954..17727
                     /locus_tag="ACG8M5_000360"
     CDS             16954..17727
                     /locus_tag="ACG8M5_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357644.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(17808..18377)
                     /gene="thiT"
                     /locus_tag="ACG8M5_000361"
     CDS             complement(17808..18377)
                     /gene="thiT"
                     /locus_tag="ACG8M5_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0357645.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(18443..18545)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18551..19522)
                     /locus_tag="ACG8M5_000362"
     CDS             complement(18551..19522)
                     /locus_tag="ACG8M5_000362"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0357646.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(19616..20989)
                     /gene="glmU"
                     /locus_tag="ACG8M5_000363"
     CDS             complement(19616..20989)
                     /gene="glmU"
                     /locus_tag="ACG8M5_000363"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0357647.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(21492..22310)
                     /gene="purR"
                     /locus_tag="ACG8M5_000364"
     CDS             complement(21492..22310)
                     /gene="purR"
                     /locus_tag="ACG8M5_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0357648.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(22477..23301)
                     /locus_tag="ACG8M5_000365"
     CDS             complement(22477..23301)
                     /locus_tag="ACG8M5_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0357649.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(23274..23954)
                     /locus_tag="ACG8M5_000366"
     CDS             complement(23274..23954)
                     /locus_tag="ACG8M5_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357650.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(23977..24915)
                     /locus_tag="ACG8M5_000367"
     CDS             complement(23977..24915)
                     /locus_tag="ACG8M5_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0357651.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(25160..26011)
                     /gene="ispE"
                     /locus_tag="ACG8M5_000368"
     CDS             complement(25160..26011)
                     /gene="ispE"
                     /locus_tag="ACG8M5_000368"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0357652.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(26165..28738)
                     /gene="mprF"
                     /locus_tag="ACG8M5_000369"
     CDS             complement(26165..28738)
                     /gene="mprF"
                     /locus_tag="ACG8M5_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0357653.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            28879..29685
                     /gene="proC"
                     /locus_tag="ACG8M5_000370"
     CDS             28879..29685
                     /gene="proC"
                     /locus_tag="ACG8M5_000370"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0357654.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(29764..31320)
                     /gene="rny"
                     /locus_tag="ACG8M5_000371"
     CDS             complement(29764..31320)
                     /gene="rny"
                     /locus_tag="ACG8M5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0357655.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(31731..32780)
                     /gene="recA"
                     /locus_tag="ACG8M5_000372"
     CDS             complement(31731..32780)
                     /gene="recA"
                     /locus_tag="ACG8M5_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0357656.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(32889..34130)
                     /locus_tag="ACG8M5_000373"
     CDS             complement(32889..34130)
                     /locus_tag="ACG8M5_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0357657.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(34370..34948)
                     /gene="pgsA"
                     /locus_tag="ACG8M5_000374"
     CDS             complement(34370..34948)
                     /gene="pgsA"
                     /locus_tag="ACG8M5_000374"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0357658.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(35157..36080)
                     /locus_tag="ACG8M5_000375"
     CDS             complement(35157..36080)
                     /locus_tag="ACG8M5_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0357659.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(36819..38105)
                     /locus_tag="ACG8M5_000376"
     CDS             complement(36819..38105)
                     /locus_tag="ACG8M5_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0357660.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(38098..39366)
                     /locus_tag="ACG8M5_000377"
     CDS             complement(38098..39366)
                     /locus_tag="ACG8M5_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0357661.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            39618..40079
                     /gene="mscL"
                     /locus_tag="ACG8M5_000378"
     CDS             39618..40079
                     /gene="mscL"
                     /locus_tag="ACG8M5_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0357662.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(40138..40584)
                     /locus_tag="ACG8M5_000379"
     CDS             complement(40138..40584)
                     /locus_tag="ACG8M5_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357663.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(40677..40922)
                     /locus_tag="ACG8M5_000380"
     CDS             complement(40677..40922)
                     /locus_tag="ACG8M5_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0357664.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(41037..41615)
                     /locus_tag="ACG8M5_000381"
     CDS             complement(41037..41615)
                     /locus_tag="ACG8M5_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0357665.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(41692..42222)
                     /locus_tag="ACG8M5_000382"
     CDS             complement(41692..42222)
                     /locus_tag="ACG8M5_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0357666.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(42226..43086)
                     /gene="rlmB"
                     /locus_tag="ACG8M5_000383"
     CDS             complement(42226..43086)
                     /gene="rlmB"
                     /locus_tag="ACG8M5_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0357667.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(43079..43474)
                     /locus_tag="ACG8M5_000384"
     CDS             complement(43079..43474)
                     /locus_tag="ACG8M5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0357668.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(43471..44883)
                     /gene="cysS"
                     /locus_tag="ACG8M5_000385"
     CDS             complement(43471..44883)
                     /gene="cysS"
                     /locus_tag="ACG8M5_000385"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0357669.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(44880..45416)
                     /gene="epsC"
                     /locus_tag="ACG8M5_000386"
     CDS             complement(44880..45416)
                     /gene="epsC"
                     /locus_tag="ACG8M5_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0357670.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(45502..45741)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(45738..47198)
                     /gene="gltX"
                     /locus_tag="ACG8M5_000387"
     CDS             complement(45738..47198)
                     /gene="gltX"
                     /locus_tag="ACG8M5_000387"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0357671.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(47368..48525)
                     /locus_tag="ACG8M5_000388"
     CDS             complement(47368..48525)
                     /locus_tag="ACG8M5_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0357672.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(48559..49929)
                     /gene="radA"
                     /locus_tag="ACG8M5_000389"
     CDS             complement(48559..49929)
                     /gene="radA"
                     /locus_tag="ACG8M5_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0357673.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(49992..50519)
                     /locus_tag="ACG8M5_000390"
     CDS             complement(49992..50519)
                     /locus_tag="ACG8M5_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0357674.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            50749..51555
                     /gene="proB"
                     /locus_tag="ACG8M5_000391"
     CDS             50749..51555
                     /gene="proB"
                     /locus_tag="ACG8M5_000391"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0357675.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            51552..52799
                     /locus_tag="ACG8M5_000392"
     CDS             51552..52799
                     /locus_tag="ACG8M5_000392"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0357676.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            52875..53219
                     /locus_tag="ACG8M5_000393"
     CDS             52875..53219
                     /locus_tag="ACG8M5_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357677.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(53336..53662)
                     /locus_tag="ACG8M5_000394"
     CDS             complement(53336..53662)
                     /locus_tag="ACG8M5_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357678.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(54277..55479)
                     /locus_tag="ACG8M5_000395"
     CDS             complement(54277..55479)
                     /locus_tag="ACG8M5_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0357679.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            55596..56111
                     /locus_tag="ACG8M5_000396"
     CDS             55596..56111
                     /locus_tag="ACG8M5_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0357680.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(56310..56756)
                     /locus_tag="ACG8M5_000397"
     CDS             complement(56310..56756)
                     /locus_tag="ACG8M5_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0357681.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(56803..58281)
                     /gene="msr(C)"
                     /locus_tag="ACG8M5_000398"
     CDS             complement(56803..58281)
                     /gene="msr(C)"
                     /locus_tag="ACG8M5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0357682.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(58635..59264)
                     /locus_tag="ACG8M5_000399"
     CDS             complement(58635..59264)
                     /locus_tag="ACG8M5_000399"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0357683.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(59248..59712)
                     /locus_tag="ACG8M5_000400"
     CDS             complement(59248..59712)
                     /locus_tag="ACG8M5_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0357684.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(60199..61467)
                     /locus_tag="ACG8M5_000401"
     CDS             complement(60199..61467)
                     /locus_tag="ACG8M5_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0357685.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(61698..62435)
                     /locus_tag="ACG8M5_000402"
     CDS             complement(61698..62435)
                     /locus_tag="ACG8M5_000402"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0357686.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(62502..63071)
                     /locus_tag="ACG8M5_000403"
     CDS             complement(62502..63071)
                     /locus_tag="ACG8M5_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0357687.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            63199..64071
                     /locus_tag="ACG8M5_000404"
     CDS             63199..64071
                     /locus_tag="ACG8M5_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0357688.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            64413..65285
                     /locus_tag="ACG8M5_000405"
     CDS             64413..65285
                     /locus_tag="ACG8M5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0357689.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            65282..67240
                     /gene="nagE"
                     /locus_tag="ACG8M5_000406"
     CDS             65282..67240
                     /gene="nagE"
                     /locus_tag="ACG8M5_000406"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0357690.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(67572..68423)
                     /locus_tag="ACG8M5_000407"
     CDS             complement(67572..68423)
                     /locus_tag="ACG8M5_000407"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0357691.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            68546..69013
                     /locus_tag="ACG8M5_000408"
     CDS             68546..69013
                     /locus_tag="ACG8M5_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0357692.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(69276..69758)
                     /locus_tag="ACG8M5_000409"
     CDS             complement(69276..69758)
                     /locus_tag="ACG8M5_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0357693.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(69858..70721)
                     /locus_tag="ACG8M5_000410"
     CDS             complement(69858..70721)
                     /locus_tag="ACG8M5_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0357694.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            71319..72194
                     /locus_tag="ACG8M5_000411"
     CDS             71319..72194
                     /locus_tag="ACG8M5_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0357695.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(72339..72491)
                     /locus_tag="ACG8M5_000412"
                     /pseudo
     CDS             complement(72339..72491)
                     /locus_tag="ACG8M5_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(72759..74090)
                     /locus_tag="ACG8M5_000413"
     CDS             complement(72759..74090)
                     /locus_tag="ACG8M5_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0357696.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        21070 a        15832 c        13176 g        24087 t
ORIGIN      
        1 taaaaaaaga aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa
       61 tataatcatg ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt
      121 tactactgct aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac
      181 aaagatgatc atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac
      241 atacatcaac catacaatta aagggagagc tactgcatag ataccgataa cgccaatata
      301 gtaagcgtca tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa
      361 cgccatactg caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc
      421 tttcgaccac tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa
      481 aacgattggc agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag
      541 ccgttggaca atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac
      601 ttcccgatcc agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt
      661 agctggagaa ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt
      721 atcaccagtt aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa
      781 accaaacgct tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt
      841 agaggatgac agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat
      901 caatcctttt tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc
      961 tacgatcagc atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt
     1021 ttcgtaacta gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct
     1081 ctcgtaattc ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg
     1141 ctcattttcg gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca
     1201 ccgtactggg gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg
     1261 gaagaaattg ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac
     1321 cacgatgata acaataacca aggtaataat aatattttta ttaggattga acttcttcac
     1381 aaagacacct cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac
     1441 aaaaggtaag ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta
     1501 gcctgaaaga caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa
     1561 tcataccatc tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag
     1621 atgtcggata ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat
     1681 tcgagacggg agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta
     1741 tatgctctcc aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat
     1801 gggcaaaggg tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca
     1861 atcttccgcc cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt
     1921 ttaagatacc taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt
     1981 tggcttttgc aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg
     2041 ccagatctac tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat
     2101 aggcatcgta tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta
     2161 ataaaatttt catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt
     2221 cggctccata gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt
     2281 ttgctgtttc tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc
     2341 tacgtagatc agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc
     2401 cgaacccagg attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga
     2461 tcatggagac tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg
     2521 tcatttgaag tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg
     2581 ctccagcttt tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt
     2641 ctaacggtaa tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat
     2701 ttgggacaaa tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca
     2761 ctaactcaat gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct
     2821 tcattcttgt ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt
     2881 tttcgatttc ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg
     2941 tttctactct atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt
     3001 tgcaatggac agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa
     3061 ttgtcaaaaa gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag
     3121 gaatcagttc cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc
     3181 ctgttgctaa attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag
     3241 actgacccat aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac
     3301 ccgtatgatt gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt
     3361 cgattttttc tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg
     3421 actcgtattc caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta
     3481 cgatacttgt gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat
     3541 tttttctcgg caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca
     3601 cctgatctcc gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt
     3661 gataggtctc tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc
     3721 ctttgataag tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt
     3781 tattccatgc aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca
     3841 caaaaaagag aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac
     3901 ttattttgaa ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt
     3961 tgaagagttg cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt
     4021 ttcagaacta gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc
     4081 tttggtaatt gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt
     4141 gtttgaatat tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact
     4201 ttgtgcttcg ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct
     4261 tacgacaaaa ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact
     4321 gatgatcgaa cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat
     4381 attgaatcct tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact
     4441 actcaaagtc actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg
     4501 atcgacttct gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga
     4561 actgaattcc tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc
     4621 ttcggcatct tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct
     4681 actaatatac agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt
     4741 cttcactaca ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac
     4801 ttctaatttc gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc
     4861 aggaacaaca acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa
     4921 aacagcaaga atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc
     4981 aggcagaact tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg
     5041 catagatttg aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt
     5101 ttcattatcc atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac
     5161 tgtagaaaga tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc
     5221 cgttgcccgt aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat
     5281 agatggaaga tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa
     5341 cggtacgcta cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg
     5401 cttcgttgcc atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat
     5461 cgtattcgtc tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat
     5521 ttttacaaca ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat
     5581 ttgatggtta tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt
     5641 ttcataagga acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta
     5701 ttccatcacc agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt
     5761 tggatgaacg agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg
     5821 gtcattttga aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc
     5881 gtgcgccaaa aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg
     5941 gccatttaat tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga
     6001 acggtgatat tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta
     6061 tgagacaatg ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc
     6121 gtcaagccat ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca
     6181 tctacttcga ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg
     6241 ttcactgcca tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga
     6301 tcctccgtca attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca
     6361 ttggcaatcg tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg
     6421 ttatattccg gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat
     6481 tgaatgaacc attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg
     6541 cctagattcg ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg
     6601 tctgctaata cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga
     6661 tttgtgttac gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat
     6721 tactttttgc gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa
     6781 gtcaacattt tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc
     6841 tcaaaatcag ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag
     6901 atcgtacaag tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct
     6961 aaaatttcca attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga
     7021 atatctggtt ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga
     7081 tcaaactgct ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag
     7141 acagcagaag agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg
     7201 tgtacatcta aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc
     7261 gtcgtcttac ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc
     7321 atagacggcg ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac
     7381 aatgagctag aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt
     7441 ttttcagctg tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg
     7501 cgtgtatcta ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca
     7561 gtttcttcgt atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt
     7621 ttgacagaaa gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc
     7681 tgataattcc tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt
     7741 gcgatttcga ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat
     7801 tggtatacag aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg
     7861 atcaatggtg caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat
     7921 tctgtaaata aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac
     7981 aaaagattcg agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat
     8041 cgtactgatt ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct
     8101 gaacttgctg accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg
     8161 gctgtaattc attgatggct aagacggtct gtttaccgca agcaatccat agattttttt
     8221 tgtctttccg gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg
     8281 ataataattt ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc
     8341 cgcgtacttg gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc
     8401 gtgtgatgtt tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat
     8461 ccaaaatatt gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa
     8521 acatcgtccc tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc
     8581 ctgcatccat ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca
     8641 tgatggcata atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat
     8701 tgattgcccc taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg
     8761 cagtgacgat cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga
     8821 ttttctctgg ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg
     8881 gcgtaattac tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct
     8941 cgtaacctga ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa
     9001 atactagctt agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc
     9061 tgctgtatca ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag
     9121 gcacgggcta aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa
     9181 cgcacggtca cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa
     9241 ccttctacgt caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat
     9301 ttctcgcctt catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca
     9361 gcgatcccta ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt
     9421 gtctcgtccg tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg
     9481 atcaatatag gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt
     9541 caattgccac aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca
     9601 aaacatgagg aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt
     9661 ttttcactct caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca
     9721 cttcttttag ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat
     9781 ggctggcggt tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat
     9841 gcatttcctg ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat
     9901 gttgaggatt gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc
     9961 ctgaaacttg ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca
    10021 gggaggtatc agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag
    10081 caatcatttg tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct
    10141 gttcgtgtgc cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg
    10201 gataaagttc tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt
    10261 tattgccgcc acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc
    10321 agcgcattga acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa
    10381 gaacaaatcc acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta
    10441 ggacagtttg ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa
    10501 aagtagaggt ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa
    10561 gctgtgcagc tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag
    10621 ctcgtgctct tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat
    10681 atttactgcg ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt
    10741 aacttgattc atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa
    10801 tagcagatcg tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat
    10861 catatttttc accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc
    10921 ctttgaatcg ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg
    10981 ttttattttc tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc
    11041 cagtgattcc ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt
    11101 gttcgacagc atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc
    11161 ggtctttata agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt
    11221 ttccttgatt caagacagca gtactgattc ctttattttt gaaataagca attggttctt
    11281 cttggctcag ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta
    11341 attttgccca ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg
    11401 tttttacttt atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc
    11461 agcgaatcaa ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc
    11521 gctgatcaaa caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat
    11581 attctgcttt catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag
    11641 taattttttt catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt
    11701 ctagcaaacg aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag
    11761 ccataacaaa tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc
    11821 cgacttgcaa agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct
    11881 gcatagtagg tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt
    11941 ttttgtcttt tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg
    12001 atttgtctta tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga
    12061 actgtgtcaa aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga
    12121 aaaggaaaca gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca
    12181 ttttttgctc ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt
    12241 gacgttcctc ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga
    12301 ctacatttcc tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag
    12361 aatctagagt aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca
    12421 ctagagaata ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca
    12481 taagactaca tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa
    12541 tgttcactgg caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca
    12601 acagcatagt cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt
    12661 tcgattactt catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa
    12721 tctggcggag tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct
    12781 ccttggactt caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat
    12841 gacaaaggag ttccataata attcccgaca tattctgcgt attccagcat ctctccagct
    12901 tcgatcattg attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc
    12961 ccttcacgca ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc
    13021 tcaaaaattg cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt
    13081 aataatccac gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt
    13141 attttgcact ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta
    13201 aaaaagctac cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac
    13261 agccaaacat aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca
    13321 agcttttgaa cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga
    13381 aaaattttgt gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa
    13441 tggtttttga acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa
    13501 cctttaaatg tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta
    13561 ttgtatttct ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca
    13621 cccatgatct catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg
    13681 tcaattcgaa caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata
    13741 aacagttcgt tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt
    13801 tcagaaggga tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca
    13861 taatatttca ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt
    13921 tttctaagat cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg
    13981 aaccgatcgt gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta
    14041 caggacattg tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca
    14101 gttcctcgtg aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct
    14161 catcgacttt cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct
    14221 tttcatagat ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat
    14281 tctttttcaa cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat
    14341 caagtgcgct tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg
    14401 gtttttcttc taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa
    14461 ttgacacttg attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa
    14521 gtgaatcaat caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta
    14581 gattcacaaa tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat
    14641 cgttttcaac aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt
    14701 tgacactttt tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa
    14761 aaccagtcat acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt
    14821 tgcagttgtt caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct
    14881 ggcgcccaat ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt
    14941 tctttacgcc accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat
    15001 gaaaattctt gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg
    15061 tgtcaactgg ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca
    15121 gataatcaaa ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat
    15181 accttcgcca ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac
    15241 tttttatatc ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg
    15301 gcagtaaaaa atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta
    15361 cgaccctgtt cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag
    15421 ataattctaa ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg
    15481 caggtttaac agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca
    15541 tttccagata gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc
    15601 tgcctagtgt ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt
    15661 ttttcgctgg gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa
    15721 tcgcaatatc aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga
    15781 tctttcgata agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt
    15841 cttctgtttt tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag
    15901 acaccgtata taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt
    15961 tgaccatctt ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca
    16021 tttggcgtct ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta
    16081 acgcaagcac ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc
    16141 cttgtaaaat caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt
    16201 tgatatcatg ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc
    16261 ctccacctaa aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt
    16321 gatagccgtt tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa
    16381 aacaacagtt ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt
    16441 tccattcaaa agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt
    16501 tagcaatagt aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt
    16561 ttgcagaacg ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt
    16621 gttttgcttg ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta
    16681 tctccatcgc gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt
    16741 ctcgtattcg actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa
    16801 aaaatacaaa aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt
    16861 ttgtaatgga attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa
    16921 aagagattaa gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt
    16981 gataccacaa gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt
    17041 atcgtgcatt ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat
    17101 gaatcccata cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc
    17161 tcttctcgta acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg
    17221 atgctgaaag aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa
    17281 acacatcttc attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt
    17341 aatgatcagc tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct
    17401 agtaaattaa gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa
    17461 gaaacacctg ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag
    17521 gaagggatca ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca
    17581 atctatgtga ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa
    17641 atcatcgcta ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc
    17701 gatctttctt atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca
    17761 agaaactagg acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt
    17821 cgtttatctg gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc
    17881 gttacaatag ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga
    17941 ttcatccccc atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa
    18001 tatctggaaa gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta
    18061 ttatcgtcta catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa
    18121 gtataggcaa tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc
    18181 aacggaaaat gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt
    18241 cttaacgcaa aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc
    18301 tggatcggaa tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa
    18361 actctatttt ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa
    18421 gcagctttcg aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga
    18481 tacaggtacg aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg
    18541 agtttattta ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga
    18601 tacgtttgat tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt
    18661 tacgatcttc tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt
    18721 ctattctttg taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag
    18781 ttgcaccagc ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca
    18841 tatcatcgat cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg
    18901 caacatttgc ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg
    18961 ctaatttacg tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc
    19021 ctttgattcc tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga
    19081 ctggaatatc aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg
    19141 ttgcaccagc tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac
    19201 gtgctttacg gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg
    19261 cacttgctcg tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg
    19321 ggctgctcgt tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta
    19381 cttgaatttc tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg
    19441 ctgctgcaat tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg
    19501 ggtcaaagta atgttttgac atggggacct ccactttctt tttcacaact ctagaattct
    19561 acctaataaa tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt
    19621 ttagataagg gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg
    19681 ccagcgcata ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt
    19741 tagctacctc gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga
    19801 cagtcgtatg atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat
    19861 tgatttcttc tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt
    19921 tgccgatttg agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt
    19981 cagcttttgg tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc
    20041 tttcaatgac tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg
    20101 aatgtgaccc gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg
    20161 cttcgatcaa tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat
    20221 cgacaaagct tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat
    20281 ttgctttagc taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt
    20341 gataagcagc aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt
    20401 attcaccttg agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt
    20461 caaaacagta agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt
    20521 tttgttccac gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg
    20581 ttgggttttc tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt
    20641 caaaaagatt gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg
    20701 ttgttccttt ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca
    20761 attgttctgc ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg
    20821 ctccgtgtcc aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga
    20881 tatgttctac cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca
    20941 tgcgggtacc tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc
    21001 ctaaatatag tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag
    21061 tgattttctt ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta
    21121 tctatttggt aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa
    21181 aacatgaaaa gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta
    21241 caacctcttc attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc
    21301 aaaatatgag gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt
    21361 cacaacctct agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
    21421 aaaaaatgtg gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt
    21481 ttcacaacct cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga
    21541 gtatctacat ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg
    21601 aattttgatt cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag
    21661 ctcttcattc cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt
    21721 gaaccacgtt tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca
    21781 gacacatagt tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata
    21841 acgaatggcg cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct
    21901 acagtcatta cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact
    21961 tgacgcagca gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc
    22021 agacggtctt gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca
    22081 tatgaaattt cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt
    22141 ccataccctc gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat
    22201 ttagcagatt cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc
    22261 aataaatact gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac
    22321 tccttttaag aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat
    22381 tttatgaaaa aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa
    22441 atttttcgtt tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc
    22501 aaccaatatt cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat
    22561 tgttgctcct ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag
    22621 aaatcctaca caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc
    22681 gattgctgcg ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat
    22741 cacagcaata gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag
    22801 cccatccacg tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa
    22861 aaagaacaat ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt
    22921 aatggtcacg atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct
    22981 caaattcatc aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat
    23041 ttctgaatag gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac
    23101 gactagcatg gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc
    23161 taaagagaca tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac
    23221 acctagcatc ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa
    23281 ttcatatgaa agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc
    23341 gcatactgtt tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca
    23401 tgcgcactat gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt
    23461 gttggttcat caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt
    23521 cgctgctttt gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct
    23581 acagcttcta aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag
    23641 cggcctcttg gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca
    23701 ttgaaagatg caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt
    23761 ttctcagcaa tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc
    23821 aaggttgact tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca
    23881 tgataagaga catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt
    23941 tcgatataac gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc
    24001 tttcagattt ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt
    24061 caagctttct aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc
    24121 agctaccttc gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac
    24181 aaattctttt agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga
    24241 aatcgctact tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt
    24301 gacaaatgtg cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc
    24361 aagctttttg atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt
    24421 ttcgcctaat tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca
    24481 aacatgtgga tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc
    24541 tgcttcttgc gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt
    24601 gctatccact gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac
    24661 gaaaacatct gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg
    24721 ctccgttcca gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt
    24781 aaaatcatac atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc
    24841 cttgttcgta tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa
    24901 aagatatttt ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt
    24961 aacgtgaact aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga
    25021 tttaagcatg tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc
    25081 catttcacta caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac
    25141 ctattttact tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac
    25201 cttttaaccc attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg
    25261 ttggtccgct tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga
    25321 tttgatggat gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt
    25381 tgatcatctg ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat
    25441 gttggatggc ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg
    25501 gtttgaccaa cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt
    25561 tgccaccgac aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca
    25621 aaccgatttc tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc
    25681 ttagagcagc tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct
    25741 tcttaacaaa gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag
    25801 cttgataaac attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga
    25861 ttttgtcttc tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca
    25921 ccatttccaa ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta
    25981 tcttggctgg cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg
    26041 caagtattct atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt
    26101 tgtcatcgtt tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg
    26161 attcttataa ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc
    26221 ctatcagact gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa
    26281 aataacgtcc ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg
    26341 aatagaaaaa attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa
    26401 aatctgtttc tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca
    26461 ttttctcgta tacaataaac agattggcta acagatagtc agctaattcc tcagattctt
    26521 taggtaagac ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga
    26581 tgaagccaac gactttttga tctttacgga caataccgac accgctagat aatacatatg
    26641 atttatcaaa tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg
    26701 aaacttcatt tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt
    26761 atccttcttt catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg
    26821 ttgctagatc aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa
    26881 gcatcactac gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg
    26941 cttgtttcat aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc
    27001 ctaaaacaaa gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt
    27061 cttcttggta ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc
    27121 ctccgtatgt ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg
    27181 tagtatcttc agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac
    27241 caataagccc tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta
    27301 ttcgaccgac taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa
    27361 agccgaacaa gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat
    27421 aaaattcttt tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact
    27481 gccaagataa cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag
    27541 gcagaaatgc ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc
    27601 caaccaatag attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa
    27661 atagccgact gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg
    27721 caaaatagac agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa
    27781 tgttatctaa aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg
    27841 tcataaaagg tagaacataa taaaacaacc gataatataa tagccaaact actgctacat
    27901 cttgccgaac agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc
    27961 cacctgggac catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg
    28021 aaagcatcga aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc
    28081 ccgtccattg tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga
    28141 agaacaatgt ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac
    28201 cgccgaaaag ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat
    28261 caataaatgt aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg
    28321 atacagtggc taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac
    28381 ttgctcctac cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag
    28441 caagaagaaa ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg
    28501 tcacccaatc atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca
    28561 ggatcgtcat gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat
    28621 tagcaatatt tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt
    28681 ttaaaatcag actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat
    28741 tttcccattc ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta
    28801 atttttccac tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat
    28861 gctaaaggag aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct
    28921 attattgatg gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt
    28981 ggacattttg aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt
    29041 aatcaggaag ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt
    29101 gaaaaagtat tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct
    29161 tcaggaaaat cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta
    29221 cgtgtgatgc cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat
    29281 gaagctgcca cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc
    29341 gtctatcctt tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc
    29401 gcattcgttt acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc
    29461 aaacagacag ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt
    29521 tcaagtgata aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact
    29581 gttgcaggtg tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc
    29641 aatacgacga tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt
    29701 ttcgtgcctt acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt
    29761 ttattattta gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc
    29821 aggataatcc agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc
    29881 taaatccgag atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc
    29941 gtaacttgat tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg
    30001 gatgtagttt tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc
    30061 tgcagctaca aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat
    30121 cgcgttgatt actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac
    30181 gtgagatcct tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg
    30241 ttttgctaat tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac
    30301 ctcgaccgaa tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat
    30361 gatcttgatt agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc
    30421 gtattcgcga atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg
    30481 cgctggatgg attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct
    30541 tcggattgga tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc
    30601 gataccagtc agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc
    30661 tttcatttca tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc
    30721 tgcgcagcgt tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt
    30781 ggcacgctgt tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt
    30841 cgatttcatg atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc
    30901 tttttgttga tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg
    30961 ttttgaacta agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc
    31021 atctttacga ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc
    31081 taacttactt tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt
    31141 agcttctaac aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat
    31201 tccagcagct gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat
    31261 aaaccctaaa ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat
    31321 aaaatccacc tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat
    31381 tattctaaac tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt
    31441 tattctaatg tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac
    31501 ttattcatta aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt
    31561 aaattgagaa acttttctat taaattattc tttgtagatg aacagatatt tttattttat
    31621 gtacccactc gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt
    31681 caaggagagt acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct
    31741 actaaaggca attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata
    31801 ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg attagccata
    31861 tagattttag cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca
    31921 ccgcttttat caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag
    31981 atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt
    32041 tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt caattgttct
    32101 gcacgacgta cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg
    32161 atttctggat tcgcaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg
    32221 attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt
    32281 gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa
    32341 gcagcaactg aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg
    32401 atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca
    32461 cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt
    32521 ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt
    32581 tcaggtccat atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca
    32641 tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat
    32701 ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct
    32761 gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt
    32821 tcattatcct tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt
    32881 cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca
    32941 ttatatctga tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct
    33001 ttagatgaaa tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca
    33061 gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc
    33121 atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc
    33181 tccagaaaac cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca
    33241 gatacaccag caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct
    33301 gctgttacag tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta
    33361 tcatcgccgt atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct
    33421 aaaagagctt gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact
    33481 tcatttggtt tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt
    33541 agctcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt
    33601 ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt
    33661 ttcaactcat taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa
    33721 gtccctaaag caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac
    33781 tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt
    33841 ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt
    33901 gtcaggtcat cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt
    33961 tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa
    34021 taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg
    34081 acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc
    34141 tcccttatat agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc
    34201 tgtgacaaaa atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac
    34261 ctcggtcata agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt
    34321 gtgactcaga gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc
    34381 ctttgaacac accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca
    34441 gacaagcata aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat
    34501 tgataaacag caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag
    34561 ctgcagccat gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt
    34621 cacgacagac aatgatcgct actacccaac caggggcctt tccttggtca accaacatga
    34681 taaaagcagt cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag
    34741 tgacaaggcc tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag
    34801 caaaaataac tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt
    34861 gtccccaatc taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa
    34921 gtacagttaa tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg
    34981 actatcagcg aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag
    35041 tatttttatt gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc
    35101 acgatccttc ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat
    35161 tctgcgtatt gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta
    35221 actggtacgg attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg
    35281 gtcgcatttg ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc
    35341 ccttgataaa cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta
    35401 aattcaaggg ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa
    35461 tcttctcctg tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact
    35521 gtagaagact ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct
    35581 tctactttaa gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa
    35641 gtcatatagc ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga
    35701 agacttgtcc aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta
    35761 cttactgttt caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca
    35821 aaaacatcga ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat
    35881 gcccgcacgt aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa
    35941 taacgttttt gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg
    36001 cgtgcttgcc gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc
    36061 tgtttgattc tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga
    36121 caaaagtcat gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg
    36181 gtcacaagtc acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga
    36241 ctcggagtta aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc
    36301 tcggtattag ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa
    36361 tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca
    36421 tctgcggtaa taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt
    36481 cttattactt gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt
    36541 agcttcttcg gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc
    36601 cttcttattt cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt
    36661 ttattttaat ataagttgat taaaagagag catgatttgt tttattttct cactaatttt
    36721 tccaacacaa ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct
    36781 aaaacgaaat ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac
    36841 tcgctgaagg cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg
    36901 atcgaatcga tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta
    36961 aattgattgg caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc
    37021 ttcttcagca aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg
    37081 atttctttga ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca
    37141 aagtgaaatt cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga
    37201 ttgtacatcg ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg
    37261 attgctgttt tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta
    37321 ataccaagta caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt
    37381 tcttttgtgt tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct
    37441 tcttggtttt cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat
    37501 aaaaccatat tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg
    37561 attttgtcga tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac
    37621 aagttattta gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt
    37681 tcctgaccga tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt
    37741 acaaaatcga tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag
    37801 ctggttttcg taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga
    37861 aagacatcac catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct
    37921 ggaactttca ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa
    37981 ttcgtactga acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc
    38041 aaaccgtttg gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta
    38101 ttcatttgca tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac
    38161 ttgttgtatc tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa
    38221 ttttgtttcc ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc
    38281 tagagaaagc agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt
    38341 tatttcacca tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg
    38401 gttcttttca tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact
    38461 agaggcataa taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg
    38521 aaaaccgcca aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata
    38581 cacatttgtc tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg
    38641 ttccttcacg atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg
    38701 cgactcaaat ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac
    38761 aacaaaaatg tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact
    38821 agcagtgatc ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct
    38881 gttttcaaaa taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa
    38941 actttccaaa tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg
    39001 atgattttta atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa
    39061 aaggtttgga tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg
    39121 taaatttcct ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg
    39181 cgcacttaac tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag
    39241 aagtgaacgt ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg
    39301 gatcgttttg tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata
    39361 caccatattt atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa
    39421 cctgcctttt ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt
    39481 ttctaaatga tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac
    39541 ggctaaaagc gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa
    39601 agaagggagg gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct
    39661 tggatcttgc tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg
    39721 tgcaaggctt gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg
    39781 atttagaagg agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt
    39841 ttggtaacgt tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag
    39901 ttgtcaaagc ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg
    39961 aagaaactcc tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag
    40021 tggcagaaga caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat
    40081 aaagaatcta aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta
    40141 acttgctact tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc
    40201 tgagattgcc ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat
    40261 cacgattggt ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt
    40321 tcttcctaaa ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact
    40381 ctccgcttct tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa
    40441 cgtatgccaa gcttctccat caatgtcatc aacttcttga taaggacact gttgttccac
    40501 aacaaaaact gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc
    40561 aaatttcttt acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata
    40621 ccgattcgca cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa
    40681 actgcttcag ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa
    40741 gatactcgtt caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag
    40801 gtttctgtta aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac
    40861 atgattgggc tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc
    40921 atacagttca cctctactat caactatacc acatattttt aaaattttca agtttaccat
    40981 aaaaaaaaaa gaaatgcaat aaaaataaat aagaagctca ttaagatttt tctaaattat
    41041 tcctcattat aaaatttcaa gtgttttttt attttgagtt ttactctccc gtaagcgctc
    41101 attacttgct tgtgatccat tgctaaacat tcagccatgt ctacaaaatc aacgccttgc
    41161 atataataat gaaaaatttt agcttctatt tttgaaaact ggatttcgca attttcgatt
    41221 gcttctttta ataaaagatg ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa
    41281 aagtctggtc cttctgtctc tattttttgg tcgattgaga cttcatcttt ttgatcacga
    41341 cgcttcagcg cgttctgctt cctcaacaga gaatagattt gattgcttat aacgctttta
    41401 taataacttc caaagctaac acctttgctt ttttcgtaag ttctcaatgc ctgatataaa
    41461 gcgatccttg cttcttgttc ccaatcctca gaaacataat ttcttaaata ataggtgttt
    41521 ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca
    41581 ccattacgtg cagcgcgcaa taactcctca gacataaaat aattcctcca taaattcgtt
    41641 tatggaggaa tcttatcatc aatgattcat cgaactgatt agaacgatat gttatttatt
    41701 ttttctcatt aaatcgtcca atttttggga caattttttt aactgctctt cattccatgg
    41761 agaattacgt cggaaatcaa taaaatgcat atctttattt tcttgggcta ttgttttttc
    41821 cgtttttttt atcgtcttat ataattcata agcagaagtt cgtaatgctc cttgggaaaa
    41881 gatcatccac tgttctgcta aatcgcttgt cgccactgtc acttgtgtca aacggtcatt
    41941 cagctgacca gcgattctct cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac
    42001 aacatccaac tggtatttct tataattttg ttggatgccc ggaactagtt gggcatcgaa
    42061 gacaacgatg acttccattc cttcatattt cgcataattt gataaacgat gtaaaagagc
    42121 ttctcttgca tccgccattt tgtcctgctt tttaagctgg acaagttccg gccacgaacc
    42181 aatcatgttg tagccgtcta caatcaacag ctgttttttc atggtctact ccccctgacg
    42241 ttgggaaaaa gcctgataca taagcaaacc agcagcaaca ctggcattta agctctggac
    42301 gtgcccggtc atcgggatcg tcagcaattc atccatttct tttttcaatc ctggactcat
    42361 tcctttgcct tcattaccaa tgactaaagc aattgatcct ttggcattcc atttttggaa
    42421 gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct gtttctttga gctgttgcac
    42481 agcttgtacc aaattcgtca cgcgtgcaac agggacatgt tcgactgccc cagtggaagt
    42541 ctttgttacg ataggtgtga ttccaactgc tcggtgtttt ggtatgatca caccatcgac
    42601 gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg tgtggatctt cgatgctatc
    42661 caaaatcaaa aagaaggggt cttctttttt ctcttttgtt cgttcaatca gttggtcaag
    42721 ggataaatat tcataaggtg taatcgcaag aacgactcct tgatgcacgc cgtgatcgct
    42781 caaagtttct aatttttgtt taggaaccca tttgactggc acagcttgtt cacgtgcgat
    42841 ttctttcagt tcttctactt tttctcctcg tgaatcttct tgaagaaaga gtttattccc
    42901 tcgcccttgt tgtagcgctt ctactgcggc atggtgccca aagacgaaat tatcttctaa
    42961 tgcttcttca ttttgatcag aaactgactg atttcgtcca gtttgctttt tatttgaata
    43021 tctattttgt tttttgtctt caaatccacg tttcgattga tgtttgtttg ttctttcttt
    43081 acccattttt ttcaccaact ttctgaatac accagcggat caattcttcc atccgctcgg
    43141 tttgttttgt caaatgcaga tagcccatga gcgcttcgaa ccctgttgcg acccgatacg
    43201 tcgtaacatc agcatttttt gcgctcgtat ggcttttagc atttcttcct ctgcgataat
    43261 aaagttcttc ttcttctgtc aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt
    43321 gtgcattcgc agaaacataa ttcgttgccg cacgatgcag cttattgggt tttgtttctc
    43381 cttgatcaat caggtggtca cgaacataga tttcgtaaat cgcatctcca acataagcca
    43441 aggctaaacc gttcaattgt gtgtaatctc tcatgcttct cttctccatc gggttccttg
    43501 cggtgtatct tctaataaga tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg
    43561 gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg atcaattgtt caatctcttc
    43621 atctaataac tcttctgctg taaataaaat accgaagatc gaaagccatt gcgagaataa
    43681 agcttctcct tgtgccaaga cagcggcaga aactttcggc tggtctagat aagtgttcag
    43741 ccacttagca agctcataga ccactgtgat cccatttgct gcattaaaat catcgtccat
    43801 ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg attttatccg catcatctgc
    43861 caaattctct gaggcagttt cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg
    43921 ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg tagcggatcg gacgacggta
    43981 ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca acgtttttga tcatttcatg
    44041 tacagtgatg aaattcccta gagatttact cattttttca tcgtcttcgc caatcgttac
    44101 atagccattg tgcatccagt aattagcgaa ttttttccct gttttcgctt cactttgtgc
    44161 gatttcattt tcatgatgag gaaattctaa atcttgcccg ccaccatgga tatcgatcgt
    44221 ttctcctaaa tgttttgttg ccataacgga acattcaata tgccaaccgg gacgtccttt
    44281 gccccatggt gaatcccaag aaatctcgcc aggttttgct tgtttccata aagcgaaatc
    44341 taatgggtct tctttttgct gctgttcttc ccctgtacgc tgacttgctc cgacttccag
    44401 ttcatcgatg gattggtcac ttaatttccc ataattttca aatttacggg tacggtaata
    44461 cacatcacct gaagattcat aagcatagcc tttttcaacc aacacttgaa taaatgctaa
    44521 aatatctggc atatgatcca tgactcttgg atgcagtgta gcaggttcga cattcaacgc
    44581 tttcgtatct tcttcaaatg ctcggatgaa acgatcagca acttctggtg ctgtaatacc
    44641 taattcgttt gctgtacgga tgattttatc gtctacatcc gtgaaattcg atacgaaatc
    44701 gactttatag cctcgatatt caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg
    44761 agcatttccg atatggatat aattatacac agtcggtccg cagacataca taccgacatg
    44821 tcccttttgt atagggatga attcttcttt ttttctggtc aatgtattgt agatttttat
    44881 cattctgatc tcctcacttt ttctcctttg attcgaacga cttttgctgg tactcctacc
    44941 gctgtagcgt cgtctggaac atctttcaac acaacggcac ctgcaccgat tttggcgttt
    45001 tttccgatcg ttaccggtcc taatatctgc gcattcgcag agagcatggc tccttgttta
    45061 acagttgggt gtcttttacc cgtttctttt cctgtgccgc ctaatgttac gccatggaat
    45121 aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga cgattcccat tccatggtcg
    45181 atgaacacgc cagttccaat cgttgctcct ggatggatct caacaccagt taggaaacgc
    45241 cagaattgag cattgatctt cgccaataaa aagagccggt gctgatataa aaaatgagag
    45301 aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca aaacgatctc agctgtcgaa
    45361 cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc gggtaaacca actcatgact
    45421 ttccactcct ttcgtaagaa ttttatgtag gaaatcatct tcttataaag aaaagaagcg
    45481 ccttttaaat aagtttcctt atcaaaagac gctttccgcg tggttccact tttcttcata
    45541 atcaatgatt atctctgaca gctaacgcct gttatacgtt cttggctact ttgtttccta
    45601 taaaacagtt tttcctgttt tctgtgtttc gccaagacca ctcccagagg catttcggca
    45661 accattcatt gataccttgc accacccggt atctctcttg taatgatcag ctgcttactt
    45721 ttttctgttc atcgtgttta gttcagtact tgatttaaat gtgctaatga tttttcacga
    45781 ccaagcaatt cgatcgtttc aggcaattct ggaccatgca tttgtcctga tacagctaca
    45841 cggattggca tgaaaagatt ttttcctttt acgccagttt ctttttgaac agctttgatg
    45901 gcagctttga cagttggagc atctactact tccatttgtt ctagctgtgc tttgaatgcg
    45961 tggagaacag taggaactgt ttcaccagca agtacttctt ttgctgcttc attcaataca
    46021 ggatgttcat tgaaaaatag cgtagacaga tcaacgattt ctgcagcata gctcatttga
    46081 ggttgataca ggctaacgat cttcttcacc cattcgattg tcttttcatc tgggttttct
    46141 gctacacgtc catcagcgat tagataaggc agacacatgt cagtaaatgc atctagatcc
    46201 attgctttga tgtaatggtt gtttacccat tgcagttttt tcgcatcgaa agctgcgggt
    46261 gatttactta ggcgttttgg atcgaacatt ttgatcaatt cttggtgttc gaagatttcg
    46321 tcttcgccca caggtgacca gcccaataat gcaataaagt tgaacattgc atctggtaga
    46381 tagcctaatt cgcgatattg ttcgataaat tgcaagattg attcgtcacg tttgcttagt
    46441 tttttacctg tttcactgtt gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag
    46501 ccaaatgctt catagatcat cagttgtttt ggtgtattgg caatatggtc atctccacgt
    46561 aaaacgtgag tgattttcat caaatgatca tcgaccgcaa cagcaaaatt ataagttggc
    46621 ataccatcgc gtttttggat gacaaagtcg ccaccgatat tatcggattc gaacgaaata
    46681 gcgcctttca ccatatctgt aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca
    46741 acagattcta gaccttgtgc ttctttttct gcttgttctt caggtgtcaa gttcgcacat
    46801 gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat
    46861 tcttcttctg tgcaatagca tttgtatgct cgattgctag caagcagctg atcaatcagc
    46921 ggttgataga tttctttacg ttcagattga cggtaagggc cgtattctcc aggattttca
    46981 ggagattcat cccaattcat tcccaaccaa gcaaggtttt ccaactggct tttttcgcca
    47041 tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa taataaagtc tccatcattg
    47101 tggcgggcaa acaaataatt gaataatgca gttcttgcat ttccaatatg caggtgccca
    47161 gttggacttg gtgcatagcg tacgcgtact ttcgtcattt tcttcctctt ctttcaaact
    47221 agactttgac taacagcaaa aattccgtca gttacggctt ctcaaatatc atttatttaa
    47281 agccactcag cacaaatttt accttttcta aggtaaatag taaagccaac tggttgaatt
    47341 tccttaccag ttggctgaat ttctttttta ttttgattgt ttagtctttt tctctgtctc
    47401 ttttttattg tcgattcctc gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc
    47461 agctgtttga agggcacttg ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc
    47521 atcttctacg acaaccatcg tgccgtcatc aaggtaggct actccttgtt ggcgttctgt
    47581 cccggctttt actactagaa cattcatcgt ttcaccaggg atgacgactg gttttacggc
    47641 atttgccaac gcattgatat taagaacagg tacattttgg aattcagaga ctttattcaa
    47701 gttatagtca ttggttacaa tgacaccatc taaaagctta gccaatttga tcaatttgct
    47761 gtctacttca ctgatatctt caaagtctcc atcgtacatt tcgacagata taccctcttc
    47821 tttttgcaag gcatttagga tatctaatcc gcgacgacca cgcacacgtt tcaaactatc
    47881 ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa ttagggatca tcaaggttcc
    47941 ttctaagaat ccagtctttg taatatcata aatccgaccg tcaatgatca cgcttgtatc
    48001 caagatttta tatttatgga aattttctcc tgattttcgt tctaacagtt gactctcaac
    48061 ttgactagct gtttcttgct cgattttttt gcttcgtgaa ccaaaaactt tcttccattc
    48121 ttcgattcgt gtcgttccca ttctcaatcc cagatagcct aagatgatca tcagtaaaat
    48181 cggcaataca ctattgacga atggaatttc catattgtaa agaggggtcg agatcacaac
    48241 acctaaagtc aatccgatga tcgcaccgat cgcaccgaag agaagataag tcaagctgac
    48301 ctcacttagt ttttgttcta cttgtttgat aatagctgag atatgtttgg ccaacagcaa
    48361 agataataaa aagaaaataa gtgcgcctaa caggctgttg gtaaatgtat tattcagcca
    48421 tccgtttgac tgttgaccga ttgcctgcca agctgagggt aaaaatgaaa tacccaagct
    48481 tgcgccgaca atgatcatca gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt
    48541 tcgacaatca tttattagtt agcggaacac acgcttcaac gtttcagcca aagttgctac
    48601 tccaactatc tcgattcctt ttggcgcttc ccagttacct aagttgtttt taggtaggta
    48661 tacttttgtg aagccgagtt tttggacttc tttgactcgt tgttcaatat gactgacacg
    48721 tctgatttct ccagtcagtc caatttcacc gatgaaacat tccgttggct gtgttccttt
    48781 ttctttatag cttgaagcaa tactgacagc gatagctaaa tcaatcgctg gttcatttaa
    48841 ttttacccca cctgcagctt ttaagtaagc atcttgattc tgaagaagca acccagctct
    48901 tttttccagc actgccatga tcagagatac ccgattgaaa tcaagacctg ttgttgttcg
    48961 tttggcattt ccgaacatag taggtgtgac taatgcttgg atctctacca aaattggacg
    49021 tgtcccttcc attgccacaa cgatagcaga gccagttgca ccgtctaggc gttcttctaa
    49081 gaaaatctgt gaaggattag cgacttcttc caatccatgc tcatgcattt caaaaatccc
    49141 aatctcattc gttgagccaa atcggttttt tactgctcgc aaaatccgaa aactatgatg
    49201 ttgttcacct tcaaaatata acaccgtatc caccatatgt tctaacattc ttgggccggc
    49261 gatcgagcct tctttggtaa catgaccgac gataaaaatg gctatcccat ttgttttagc
    49321 ggttttgagt agttctgcag tggtttctct tacttggctg acacttcctg ccacacttgt
    49381 gatgtctggc tgcgtcatcg tttggatgga atcaatgata acataatcag gggagatatg
    49441 ttcgatggca cgactgattt cgttcatatc tgtttcagcg tatagataaa attctgtgtt
    49501 gatacttccg aggcgttccg cgcgtaattt tatctgctct gcgctttctt cgccggatac
    49561 atataagact tttccgccta tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga
    49621 tttcccgata cctggatcgc caccaatcaa gaccatagac cctggcacca cacctccgcc
    49681 taacacacgg ttcagttctt ctaattttgt tttgatccga ggttcttttt tagggacgac
    49741 gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc
    49801 cgttgtatct tgttcgattt cttcgaccat cgtattccat tttccgcaat ttgggcagcg
    49861 tcccaagaac ttaggcgaca catacccgca attttggcaa acaaattgta cttttgattt
    49921 ttttgccatt gtaccgcctc cttttatcct aatcatgaga cattaaatca agcttaagta
    49981 tctttaacgt tttatttact ggaagaacca aatccgcctt gacgttgagc agtcgtttga
    50041 tcttgatctg cttttaaaaa cggcaaaaag atcccttggc cgatacgctc gccttttttg
    50101 atcactacat ccttgaaacc aaagttggtg aattggaaca tgatatggcc ttcatttgcc
    50161 tcattattgt aataatcact gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc
    50221 ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt catcttctcc catgtaagct
    50281 ttgattccag taggcacgag ggttggtttc aattgttttt cattttctga tacatctttt
    50341 ttcaatccat ctgcgactgt ttccaacccc tttttccagc ctgctttcca gatacttgga
    50401 atcacgacct cttctgctgc ttcaaaatca tagccagcag catgatgtgt agcgcgctgc
    50461 ggtagattga tattttgatc tttatattta gaaataactt caaatcctct ttttctcacc
    50521 ggttttcctc cctaaataaa ctactcctat tctaacatat ccgaggctgt aaaagacttt
    50581 ctttacaaat tcttcaagaa attctgctat cttaaagtaa accattaatt cgttcttttt
    50641 ccaaacgact gcaagttgta caaaaaaact attttttaca tgactttact cgtcaaaaca
    50701 agtagaatag aacgaaatta atgagtggat atttgattga ggtgtcacat gcgtaaagaa
    50761 attaaaaatg caaaacgaat tattgtcaaa gtaggaacaa gtacattgat ttatcccaat
    50821 ggaaatatca atcttgcggc aatcgatcag ttagcttttg tcctttctga tcttagaaat
    50881 cggggaaagg aagtgatctt agtctcatca ggagctgtag gcgttggtat gcataaatta
    50941 catttgtcag aacgtccgac tttgattcct gaacagcaag ctgcggcggc tgttggtcaa
    51001 gccgaactga tgaatatcta taatcaaaga ttcagtgtat ataatcaaca gacggcacag
    51061 atcctcgtga caagagacgt ggtagattat cctgaaagtc gtaaaaatgt gaggaataca
    51121 ttggaacagc ttttgattat ggacacgatc cctatcgtga acgaaaacga tactgtagca
    51181 gtggatgaat tagatcatct gacaaaattt ggcgataatg atcagctgtc agccatcatg
    51241 gcaaaacttg tcgcggcaga ccttttgatc gtcctttctg atatcgacgg gttttattca
    51301 gataatccga ctacaaatcc cgaggctcag atgtatcgct atatcgataa aatcgatgaa
    51361 aagattctgg cacaagcagg tggtgctggt agcacatttg gaacaggcgg tatgcagagt
    51421 aaattaaaag ccgcttctag gatatttgaa gcaaacagcg caatgatttt agcaaatgga
    51481 aaaaatccgg caattatttt tgatattctt gcaggaaaag aagtaggtac tttatttaag
    51541 gaggacttga aatgactgac ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac
    51601 aactagcact tatgcctaca aaccgcaaaa atgagctact tgttcaaatg gcacaaacca
    51661 tcaaaaagaa tcaacaagcg atcattgaag caaatcataa agacttagaa aaagcagtcg
    51721 aaaacaatat cagtgaaacg atgcaagacc gcttgcagct gacacctgag cgaatcaatg
    51781 ccatggctac agagatcgaa aaaattgctt cgttagacga tcctattgga aaagtagatg
    51841 agatgtggac gaacaccgac ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga
    51901 tcggcatcat ttacgaatct cgtccaaatg tcacgacaga tgcagctagt ctagcattca
    51961 aatcagggaa tgccgttatc cttcgcggtg gcaaagaagc attcttctcg aatcaattat
    52021 tagtccaatt attgcagcaa gtactggtca gtgctggcga atctccttat gccatccagt
    52081 ttgtcgatga tacttctcat gaaactgcag gacagttgat gcgattgaca gaatacttag
    52141 atgtgctgat tcctcgaggc ggagcaaaat taatccaacg agtcaaagaa caggcaacgg
    52201 tacctatcat tgaaacaggt acaggaaata atcacgtata tatcgataaa gaagcacagt
    52261 tatctatggc agtgaacatc atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg
    52321 cggcagaaac attgctgatc cattctgaga tcgcaccttt tttcttgcca gctattgaaa
    52381 aagaactggt agaacacggg gtctctttac gtgcggatgc tcgtgcgtta gaatatctag
    52441 agacggctgc tttagcagaa gacacagatt gggatactga atatctagat tatatccttg
    52501 ctgtcaaagt ggtcgattct ttggatgaag caattgcaca tatcaatcgg cataatacta
    52561 aacattcaga aacgatcgtg acagatagtt atgcggctag tcaacgtttc ttaaatgaag
    52621 tggacgcggc ggctgtctat gtcaatgcct ccactcgttt tacagacgga tcggtatttg
    52681 gttttggtgc agagattggt atctctactc aaaagttgca tgcccgtggt ccaatggggt
    52741 taaatgaatt aacttccaca aaatatatca tttatggcga tggacaaatc cgcgaataat
    52801 gtttcgtgtg aaacattatt cccgcctttt gccttttttc tgtaaaggtt ctatccaact
    52861 agaaaggtgt caatatgttt gaaattttaa tgattttttt tatttacctt atttctctga
    52921 atatcgctgc ttttctggga gtcggtattt tatccctttt ctttcaattc aaaaaacgaa
    52981 gcatagggag tcaacgggaa aaatggtcgc agtattttga caaaatcggg caaaaagggc
    53041 tggttacacg cttgcacatt agttacatgg ttgcattatg cttattagcg acaatcaatt
    53101 actattcttt tttcgatcat tcaatcgctt atacgattac gctactgatt gctgggattt
    53161 ttcacttatc ttataaatat cagctgaata aaaaccactt gaatcggact ttccgctaaa
    53221 aaatatccgt acgataagta gagtgccttg aagatcggca aaaaactgcc ggacttttta
    53281 gaggctgtga caaagatttt gtcacagcct ctttttccat ataaacggcg ttcgcctacc
    53341 agctgcctgg taaatctgtt tcctcttcgt taagaagcga aaatccgtca aatgtttcgc
    53401 catcataata acgaagcagc tcatccatgg acaagttcat cgttttttct ttttcttctt
    53461 ccgctgattc agccagtagc tgttcccgat acacctgata cataccagct attttttgct
    53521 gcatcgcttt tctagtttca ccttccgcat tgattattgg caattctttg atatagccac
    53581 gataaatcaa ccgatcatct gaaaattcaa tgggtaattc aacgagtgtc aacccgactt
    53641 cctgaaaata ttcttccagc atctattatc cccccattct ttttcttaaa tgaattttag
    53701 cttacctact gaaaattttc cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa
    53761 atcgtgtcac atcccctttt tccgaataaa tggtgttctt ctgccaactc ctcaatcata
    53821 agtcacaata ttcaacaaac atgagaaact gttttttgaa attgttcctt gtgattcaga
    53881 gttaaacgac agtctcacaa cctcttggta aacggcgttc aatcagctga ccctcggtat
    53941 tagctcaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct ctaatactta
    54001 ggataaaacg ctgattttac aacctctgtt aaacggtgtt caatcagctg tccttcggta
    54061 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca
    54121 ggacaaaacg ctgattttac aacctcctga taaacggtgt tcaaaagctt gcatctgcgg
    54181 taataagccc aaccaaacga aaaatatgag aagctatttt tgtttggctg ttcttatgac
    54241 ttgatgcagg acagcttttt cacaacctct gatttcttat tcttcttcat ttgtagcaaa
    54301 ataattacgg tgataaatga tacaacatac actgataaga acaatggcac ctgcaaaata
    54361 aaacgcatag ctttcaccaa tatgttcacc aatcacacct gcaataaatg gtgctaaagc
    54421 tccaccgaaa aagcggacaa aactatagct ggacgaagca acgtttcgtt gcagacctgg
    54481 tatttccata gcaatcgtcg tcaacagtgt attgataatt ccttggaaaa atcctgctaa
    54541 cacaatgctg atcggtacaa ggactggcat agaagttcca gctcctaata acagcaaaca
    54601 aataatgaat aagacaaatg ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag
    54661 agccggtgca acgaaaatcg aagcaatagc cagaagcaca ccccagccaa aaaatacaaa
    54721 accgacttct aattcagaat agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa
    54781 gaatccgaaa ttatataaca atgcgattag tccaactgaa cgaagtttat gattgcttaa
    54841 cgctttgaca ccttcccaaa ggcctacttt tctctttggt ttctcagttt caggtaaaag
    54901 taccgctagg gctataaaac cgcaaaacat caaaaatgct accccaaaaa atggataacg
    54961 ccatgaacca ctgcccaaga ccccaccgac tagagggcca acagccattc cgcagccaag
    55021 tgctgcttca tacatcatga ttgctttttc tgtgttacca actaaaatac tgacgatcgt
    55081 tgctaaagca gtagagacaa ataaagcatt cccgacaccc cagccggcac gtaacccaat
    55141 cagcattccg atattcccag ataatccgcc aagaccagcg aatacaacga tgataaacaa
    55201 tcctgtcatc aatgtttttt tcaaaccgat ccgactagat aagaaacctg taaatagcat
    55261 caccacacca gtgactaaca tgtaactagt aaataacagg gtcgtttcag ctggcgtagc
    55321 gtttaactgt gcagcaatcg tttttaagat tgggtctact agacccactc ccataaaagc
    55381 gatcatacag gtagctgcta caactagcgc tgaacttttc ccctttgttt cttcatttgt
    55441 ttgttcttgt atatctattt gattctccaa tatttccatc cctttctctc tattagcatc
    55501 atacatgatt tattttacat gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat
    55561 ataataaaga aaataagacg agaggtagga tttttatgaa tactttagcc tatatattat
    55621 tatgcgtgct tttatccaaa ccacgttctg ggtatgaatt gaagcagctg atcacaattt
    55681 tttgggaagc ccatcacagt cagatctata caacgcttgc caagttagac cagcagggtt
    55741 atgtgaccgt tttggacaaa gacgagcact ctcaaaaaaa gatctaccac ttgactgatg
    55801 aaggacgtgc tcttgtagag gaatggatca aagaggaaac tcccgctcct acgcaaaaag
    55861 atgaatttct agcaaaaatc tatgcttttt cgacgctcga taaaacaaca gctactggac
    55921 tgctattcac tagagaacaa cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga
    55981 atgacctctc gctagacaag aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa
    56041 ttatgctttg tcagcaggaa ctccaatggt gtcagcaagt aagagaacaa atgcagcagt
    56101 tttttgagta agggataaac agaacctttc tagaaatatc tattgaagag caaattatct
    56161 tcaataaaag agcaatacag ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct
    56221 atcgttcgga tcactatttt tctagaactg cattcaagag actatgacaa aaagcatgcc
    56281 aaggcctttt ttcaaataac agtgttccat cattcattac tttgaattgc ttttagcaca
    56341 cttcttgaaa gcgggccaac gatgatcaac tgcaacggta aagcggcaat cacattcatt
    56401 ttccaagcat tcatataagc agagaataat tgatcaggta ttccttgttc catgatcaca
    56461 ccgaataaag acatacatgt caccattcct aaaaccatca agcatgaaat agttaagatc
    56521 atttggacct tgctttctgg atttactggt agtttaaagg caattttttt cgccagcaca
    56581 ccgatgatga agacatccaa aatgaaagcc accacgaatc caggcactag accaatcaac
    56641 aaacttgata aatgaagatc ttgatgcagc cataaattat acgcgctcat ccccaagacc
    56701 atcaaaaaac acatcattgt tgtaaaaatt attccttctt ttttatttgt aggcaatctg
    56761 taccactcct ttaaatttgt cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag
    56821 tacttttttt attttctgcc cttttatttc ataaacttca tcagccgtac gctcgacaaa
    56881 gtaagaatca tgagaagtaa acaaaatcgt tcccggatat atttgcatta gcttctctaa
    56941 agcttcgatc gttgccatat caataaaatt agtcggttca tctaacagca acacattact
    57001 tggcttagta aaaagcaaag caatcgtcag acgggtcgct tctcccccac ttaatgtaca
    57061 taacggtttc gtcaaagttt cctgagcaaa ccctaagtga ttcagcaaac tacggacaag
    57121 cgattctgaa aactctgttt gtctcatgac aaaacgaatg atggattctt cagatgtcat
    57181 ttgataaccc ttttgctgat atacttgaaa gcttactttc ggagagagaa ggattccttc
    57241 tccttgtttg cggatatgtt ctaataaggt tgtcttacct gaaccatttt caccgacaat
    57301 cgcgattttt ttacctaaag aaaactgaaa atcaccattt accagtaatg ttctcccact
    57361 tctttccaat tggacatttt gtcccatgat gggatagcgg ctgtggagtt caagagattt
    57421 tggtactgga aaacggattt gtttcatctg ctgcggcttg gttgtttctc ctattttttg
    57481 gagtcttctc tctaatgttt tcgcattctt ttggatggct ttttgtacac tgtctttttg
    57541 tttagaggaa gacaaccgat ccagtctgat ttgttgcttt ttctttttac tggacacttt
    57601 ttccaacttt tccgcttgtt ttttcttttc ttggatcgat ttttccaatc gttttttctc
    57661 tttcatgatc gactctgctt ctcgtagctg agtctttttc tccaattcct tttgagtgag
    57721 ataggcacta taattccccg aaaactctcg gatttctccg tcttttactt cccagatttt
    57781 cgatgctagc tcatctagaa aatagcgatc atgactaaca aaaaccagcg taccataata
    57841 ataacgaagc tcttcaatca gtcgatcaat actttttcta tctagatggg ttgttggctc
    57901 gtctagaagc atcccttgtt cataagaaga aagaagacgt atcaactcga tctttcgttc
    57961 ttcccctccg ctccatttcc gctctgaagt ttcttccagt cttaactgga aatgactggc
    58021 taattccaaa tttaggtcct ttgcttcagc gagataggtt aattgaggca ggtagttgaa
    58081 ggtgatctct gtttggattt tcccatgatc gggaaaaagt tcaccggcta ttagtttgag
    58141 caaagtcgat ttaccgacac cattttctcc tatgatccct atacgatctt gctgatagac
    58201 acgaagagaa tcaatagata gttctagctg gttgccaaag ctgacttgta gatttgttat
    58261 atttactgct aaattttcca tatgttttac actcctattt catgtaaaac aacaattatc
    58321 gatactaaaa aagagcagaa aaatctgttt gcaaaaaaac tgcatcacat cttttcctac
    58381 tctgaacgaa ataactatga ctaaatcacc aaatctgtca tagctttcac ctattctact
    58441 gtcgttcgaa aaaatggaca tactccatcc atgaaattgt tgtttataca tgtcttttga
    58501 atttatttag ttgttattca aaagttcgaa acgtaatttc atcgatgcag tcatttttcc
    58561 gacctccttt tttattaggt taccacacac gattcaaaaa aagcaatagc tactgaatag
    58621 taactgaaaa ccttttattc gaacatagtg atcttttctg gtgctaaacg aacagactcg
    58681 taaccttctt ctaccgcttt tcctattgat aaaataataa ctggaacata acggtcttta
    58741 tccaaaccaa atgcctctgc caactgatct gcttcgaatc caccaattgg atttgtttca
    58801 tagccatgat cacgcgcaac taacatgaat tgcatagccg ctaagctaga atcgactttg
    58861 actacatcgt tcatttcttc tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat
    58921 tgttgatctc tgacttcttt tggcatcttc cctgattcgt aagcttggtt gtagatttct
    58981 tcgccatatt cgtaacattg catatcgcca aaaatcatga ccattgcaga agaagtatca
    59041 ttttgtctcg tattgaaacg gatcaaagga cgcaagattt cttttgcttc ctcgctttca
    59101 gcgaccacaa agcgccatgg tttcatattg acagaagaag gtgcagtcgt cgcttctttg
    59161 atcatttcca gcatttcttc gtgtgggatt ttgtatgttt catcatatac gcgaacggat
    59221 cttctaccag tcataattgt tgaaaaatca tttttttgtt ccattattat taacctcttt
    59281 cgtttctgtt gttatattgc aaagttgggt attcaattta tctaaaagtg ttagaaattg
    59341 tttcatttct acatcgctga aagccgggga tagcacggaa cctgtatctt tatggtccat
    59401 ttcacaatgc ttgattttct tctgggcttt ttctgttagc gctacataca cttcccggtt
    59461 gttctgagga tttctctgac gcgaaacata gccatttgct tccaatacct tcagatgacg
    59521 agtaactgct ccttgatcaa tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt
    59581 ttcttctttc agtctcatca tgatctcgta tctagtaaga cttataccga tattttcttc
    59641 aaagatctgg acgatttttt gatcggctaa tttgatttga tataaaaggt gactaatctc
    59701 ttctaaagac ataatttcct cctttctaat aattgactcg tcaacgattg acttgtcaat
    59761 aatagaataa aaaaagaaaa aagtcaaaga atcagctgtg taataaaaaa aacgctctca
    59821 atagaaaagt atagagaaaa gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa
    59881 gcacaacgct aaaaccaaga ttattgattc ttatcaaact atagaagata gctcttctgc
    59941 agtagataat tttacaccgc caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga
    60001 atattagaaa ttggatcagt atgcttaaac agtaataaaa aagttatgac aagatgttgt
    60061 cacaaccttt tttgagtaaa cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc
    60121 aaacaaaaaa catgaaaatc tgtttttgtt tggctgttct tactacttga tgcagggcag
    60181 ctttttcact acctcttttt aataaccata aaatcctatt ccttcatatc tccagcccat
    60241 cggtgttagc caatctcttt ccttagcact caaggtatag tgatgggtac cagttttttc
    60301 atttggatta aatagtcgat aaattggctt ggaaccacca gaataaaaac ttagaccttc
    60361 ataacgccaa ccgatttttg ttaaagagtt gatctcagat tgtgccattg tgtaatgatg
    60421 gtctccactg taaggattgt ataaacggta gacaggttgt ccgctgtttg gtgcttgcca
    60481 agcaatccct tcatattccc agcctacagt tggcagataa tttttttctc cggcattcaa
    60541 tgtatataaa tgttctcctg tatgacggtt atacagacga tacatcggag aagttgaagt
    60601 tggtgctgta tcatactgtg tcagattata ggtttcgatt acattgttca atgtagaacc
    60661 ataattaggc gcagtggcat aacgacctgt tagccaagcc gtagcatctt tgtaagagtt
    60721 cgtattgctt ttccatgcac cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt
    60781 caataccgtt acgttatctt gcaatgattc tttgtacgaa ggatattttc taaaagcggc
    60841 attcaccgta atccattgcc cattcacata ttccgaagta ggcatcgtaa cagattggcc
    60901 attatagctt cctttaatcc caaatagatt gtaattaggg gcagaagcca aagtactgtt
    60961 cccccagcca ctttcaacga tggcttgagc aatcatgact gatgcatata agtcattggc
    61021 acttgccaaa ctttgtgcag ttgaggcaat actatcaatg aacgcttgtt gagaattagc
    61081 agatgagcga gcataaaaag catagccagt atagttttcg aattcagaca aatctgcttg
    61141 atgggtatgt gtcaatgttt gcccatctgt caattggttc gtgatttctt ctgcattctc
    61201 tggattgttc tgagcttctg ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc
    61261 agttgttggt tcttcggttg aaattgtatt ttctgagaca gtagctgact gcaaagtact
    61321 ttctgtatcg ttttcaattc ctgtttctga agaactcgat gtctgagttg cgacagtttc
    61381 tgtcgattct tcagctagca ctgttgtgtt ggcaactgct agatttccta aaagaataac
    61441 gctaaataat aaattctttg ctttcatctt ttttctcctt tgaattatga aacttttcct
    61501 aaataacagt gtaactaaaa aagtaaaagc aagtcaatgt ccatatttaa ataacatttg
    61561 tttcttttta ataaatttgc tttttctgca taaaaagacg taaatgaaga atattcttct
    61621 ccattcacgc cttaaattga tggtcgtttg aataactatt taacaagtaa ttctctctgg
    61681 atattatccg ttatggatta tacttcctgt gtcgtcggac ggataatcat gttgctgaca
    61741 gacatccggt ctggcgtttc tatcgcaaat gctacagcag aagctatatc ttcagatgtt
    61801 aatccccatt ctttttgcgc ttcatgaagt tcaagagcag cctttttgtc gctgatggtt
    61861 tgatataatt cagtttgaac agctcctgga gagatgatcg tagatttgat gttattttgc
    61921 cgttgctctt gacgtaatcc ttccatgatt gcacggacag caaattttgt tccgcaataa
    61981 acggcggaat caggataaac aacatgtccc gccacagaat cagttgaaat gatatgccca
    62041 gatttttgtt tttccatgat tggaagtacg gcggaaattc catttaacac acccatgatg
    62101 ttgatatcaa gcatattttg ccattcatca cggtgacctt caactaatgg agcggttggc
    62161 atgattcctg cattattgta caatacatcg attcgaccga acttttccat cgctaaatcg
    62221 ataactgctt ggacttgtgc aaaatctctt acatctgctg tttgaatata tagctcagct
    62281 tctggtaaag attcttttat tgcttttagg cgatcttctc ggcgtgcagc tataactagt
    62341 ttagcccctt tttctgctaa tagcttcgtt gttgcttcac cgattccgct tgaagcgccc
    62401 atgatgacaa tcactttttc tgataaatca ttcattctat tctcctcctg ttttgaaaaa
    62461 tctcttgctt atactcacta tctcttattt catcttactg ttcaaataat ccgttcgaca
    62521 ggtttgacga ttttggcagg gattcctgca acgatggtat tgtctgggac atctttcgta
    62581 acagttgcat tcgctgcaac aatggcattc tcgccaatcg ttacgcctgg cagaatagtt
    62641 gctcctgctc cgatccaagc atttttcttg atgtggattg gctttactga caagcctctt
    62701 cgttcttttg gatcggttag atggttcacc gtgatgaggt tgacacgagg accaatcaat
    62761 actgaatctt ctaaacagat gccacctaag tcaacgaaca tcacttgacg attgataaag
    62821 acatttttcc ctatactaat atgtttgccg aaatctgtat aaaaaggcag cagaacagtc
    62881 accgtatcat ctactttgtc actaatgata ttttctagcc gtttgcggat atctgctggt
    62941 tcatggtacc cagtattcag cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg
    63001 atcacttcaa agagcggatc cgttactcga atctctttat tgacgatttg tttttcgatg
    63061 aggcttgtca ttttgctccc tccttttatc cttactttat aacggacggc ttatgatgtc
    63121 taatacttat aaaacatact taactattcc ttttaactat acttagtggt atgctttttt
    63181 aaaaacagga gggaaaaaat ggatattcgt atactgacgt attttttagc agtagctaga
    63241 gaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa
    63301 caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgagg ctcccgagag
    63361 atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg
    63421 gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt
    63481 ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag
    63541 gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa
    63601 ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac
    63661 cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg gaatcatcct
    63721 ttagcaaagc aaacagtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc
    63781 cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta
    63841 tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact
    63901 tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa agtattcatc
    63961 ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt
    64021 tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt
    64081 ttttccttcg ctttcagctt tttaacaacg aataagttaa gcaaaagatc caatgattaa
    64141 ataacataag tttcttaatt tgttccttcc ttttatttct gataattcca gtaaattact
    64201 ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac
    64261 aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt
    64321 ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac
    64381 gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa
    64441 atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat
    64501 ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg
    64561 aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat
    64621 ttgcagcaga aacgatcatt gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac
    64681 atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta
    64741 tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta
    64801 ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg
    64861 aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc
    64921 gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg
    64981 aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac
    65041 gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag
    65101 aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc
    65161 tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata
    65221 tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa
    65281 attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc
    65341 tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt
    65401 cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc
    65461 cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt
    65521 ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa
    65581 tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat
    65641 tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat
    65701 ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt
    65761 gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt
    65821 tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg
    65881 tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc
    65941 aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat
    66001 gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat
    66061 ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgt tagcagcagc
    66121 ttttgcttca ttctttactg gtgtaacaga accacttgaa ttctcattta tgttcgttgc
    66181 ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt
    66241 cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt
    66301 gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt
    66361 gatttactac tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg
    66421 cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc
    66481 ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga
    66541 taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa
    66601 aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat
    66661 cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc
    66721 aacacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtggaacgat
    66781 cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga
    66841 tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga
    66901 aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg
    66961 aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt
    67021 aaaaggcgaa ccattcactt tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt
    67081 gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt
    67141 tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa
    67201 agcaaatgat aaaatcggct ctgtttccaa caaagcctga gccatcgtct tccccagact
    67261 tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga
    67321 ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagccaaac gtaacatatg
    67381 aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt ttttacaacc
    67441 tctgataaac ggtgttcaaa aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg
    67501 tggaaagcca ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac
    67561 ctctttatac attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat
    67621 ggttttccca atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata
    67681 tcatgttgga tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga
    67741 ttttcgacaa acgctgcatc acttgcttca ataatttcta actttttacc tgtcgcacgc
    67801 tctaatgcct tggctaattc ttcatagtca gttagattgc cggataattc taagatttct
    67861 ggagagtcac tttctactag tacttttgct gcagcttctg catattcacg tttcaatacc
    67921 caacctgttt ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca
    67981 atgataggta attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct
    68041 gaatccttaa tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga
    68101 tcggcatctg caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc
    68161 actacattct tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga
    68221 tctattcctt tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata
    68281 ttaaatccag cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt
    68341 tcattttgtg gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt
    68401 gctcctgtta ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt
    68461 acagctgtac atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc
    68521 aaaaaaaatg aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca
    68581 tgtacttgcc tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc
    68641 cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc
    68701 cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca
    68761 gatttcttta ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga
    68821 tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag
    68881 ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct
    68941 gatacataaa atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga
    69001 gagattttta taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa
    69061 attcttttat tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc
    69121 aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg
    69181 cattagttct gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct
    69241 caggtcaaag cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc
    69301 cagatttctc ccatatattt cccgttggta actcccattt cgactacagc atttggaccc
    69361 ccaacatagg cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa
    69421 tcatttctta attcttcagg cgattctgct aaagcgatta caacaagata cgttcctttt
    69481 gggaatggga tgattcgggt agtgtttgga acatcctgac tcgtttgaac accgacatag
    69541 tacatcattt tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct
    69601 tgtttcaatg catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct
    69661 gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc
    69721 gtaaaatctg gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata
    69781 ttaagttcat cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg
    69841 atatttttat tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca
    69901 ttactgaaat cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taactcataa
    69961 tctgaagaag gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga
    70021 aatggccctt tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa
    70081 tcagcgtacg gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt
    70141 tcttccaagc gggctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa
    70201 tctcctaact tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt
    70261 atttcttgat tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa
    70321 aactgttctt tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa
    70381 aatgtcaagc gtggaaaaga tttttgtatg ccgtatctgc tgatttctga aggcagatat
    70441 cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac
    70501 gccgtttctg tcactcttga tccatctagg agatcgcgat tagccaaagc caatctacgg
    70561 tttttgatat agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga
    70621 tattcagaga ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt
    70681 tcttcgatgt aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgct
    70741 tgccttttta tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag
    70801 tcagatgtag aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg
    70861 ataattggta ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga
    70921 cacattctaa gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac
    70981 tacctactgt acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt
    71041 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
    71101 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
    71161 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
    71221 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
    71281 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
    71341 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
    71401 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
    71461 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
    71521 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
    71581 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
    71641 gggagctttt aacagagatt cttatagaac atccgcaatt tatcgaacgc ttcaaagtta
    71701 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
    71761 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
    71821 agttcatcca agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt cccgcattcg
    71881 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
    71941 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
    72001 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
    72061 cctcttattc aaaacgctac tccataaaaa tcctgaaaga agactttcgg gaaaaagaat
    72121 tttctaacca aaagaaaaaa atagataaaa aaactaaaga aacggtaaag aaagacgatt
    72181 tcaatgaaaa atagcaagta aacgataaca accatcctaa atccacaaga aatacagcag
    72241 accttgcttt aaaaacacga atcatgcaaa ggatgaagta aagaagactt aacgcgtacc
    72301 caatattagt catttgacta ttaggtacgc gttttgtttt ataagtcatg acaaatttcc
    72361 ttcgttgctg ggtagatttt ccggcattgt tgatagattt tatgatactt ttctacattt
    72421 tgtaagagag gttccactgc atctgtatat tggataaaat tctgacaaca actttcaggt
    72481 gtgtcgaacc actctaatct ttaaagataa atattaaaat aacgattaat aatattaaca
    72541 gtctttattt ttcaccacca aaaacctatc tttgcatata ctaaatagac gtatatacac
    72601 agataggttt gtcttgtgaa aaataatgat tatccttacc aaaaaatgtt tgtttttcta
    72661 catgttcgta cttatcaaaa aatatgatcc ttattgaaat tactggctat ttatattcca
    72721 acgacttctt gtgttaattt ttgttatctc atagtaaatt atgtttcatt ttcacttaat
    72781 ttagttgtca cgtgttcgtc aattcgtaaa aacggtaaat atataaacac acttgcggct
    72841 ataattaata tttgtaaaac agctgctcgc cagtctccgg ctgttgctaa aaatcccgat
    72901 ataactggtg gcgtagtcca aggaatttgc actactacat gattaattaa tcccacagca
    72961 gttgcaaaat atgctactgt caaagaaaaa atcggtgcaa gcacaaaggg aataatcaga
    73021 ctgatattaa atacaatagg caatccaaaa ataataggtt cgcttatatt gaatatagcg
    73081 ggagttaccg ataatttagc aaagtcctta taatcttttc tacgactgaa gataaatatt
    73141 gcaatcaata aagcaatcgt atttcccgat ccacccatta ccgcaaaggt atctttaaag
    73201 gccatattga taatatgtgg aatttgctct ttgtttgcat aagctgccat attttcttgc
    73261 atattctgaa tcaaaaacgg atctaacaat gaacctgata ttactgcttg atggatacca
    73321 aatccaaaaa atagattagc aatagatgta attaacaaaa atcctggtaa accagttgta
    73381 attttactta atggctctgt aactatcgtg gtaattagct tatttatatc catattaaac
    73441 aattgaatga ttataaatga caataaagca aacgaggtaa tcgtaataat gattgggatt
    73501 aatacattaa aagactttaa aacagcggga ggaatatttc cggatagctg aatctgtagt
    73561 ttcttattat tagaaagttt aataaataat tctgtagccg ctaatccaac aaatatacct
    73621 acaaacattc ctgaagctcc tgtataggat ataggtatca cacccgtcac ttcaacagct
    73681 ttatccgcat tttcaggagt aaacatcatc tgcataggtg ttaatacaac aaatgtcgaa
    73741 atagcaacta ataccgctgc aatatgattt gtataatttc tgcttgcaca catatgataa
    73801 gatactgctg ctacaactaa taatgtagtt atgcttaatg taccattggc aattgaagta
    73861 ccaatttgtt gtaacgtggt caatgtacta gtatcgacaa tttcccccat gaatcctgtt
    73921 ggttctaaga tcactgaatt aataagtgtc acaaatcctg ctaacatcat aaatggcata
    73981 aatgtagcaa acgcatctcg caaagttctt aaatggatct gattccccaa cttagtcgca
    74041 actataccaa atttttcaaa tacaatgtcc ttcctgctac ttttgcccat attttttctc
    74101 ctttatgctt attgtaatcg attacttttt ccacccaaat aatacatcac ttatcaaaaa
    74161 aagta
//
LOCUS       DAZKMZ010000005        69071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000005 DAZKMZ010000000
VERSION     DAZKMZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.005"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..76
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            192..773
                     /locus_tag="ACG8M5_000414"
     CDS             192..773
                     /locus_tag="ACG8M5_000414"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0357697.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            776..2083
                     /locus_tag="ACG8M5_000415"
     CDS             776..2083
                     /locus_tag="ACG8M5_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0357698.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2499..2987
                     /gene="purE"
                     /locus_tag="ACG8M5_000416"
     CDS             2499..2987
                     /gene="purE"
                     /locus_tag="ACG8M5_000416"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0357699.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2980..4104
                     /gene="purK"
                     /locus_tag="ACG8M5_000417"
     CDS             2980..4104
                     /gene="purK"
                     /locus_tag="ACG8M5_000417"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0357700.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4117..5412
                     /gene="purB"
                     /locus_tag="ACG8M5_000418"
     CDS             4117..5412
                     /gene="purB"
                     /locus_tag="ACG8M5_000418"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0357701.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5534..6922)
                     /gene="ltrA"
                     /locus_tag="ACG8M5_000419"
     CDS             complement(5534..6922)
                     /gene="ltrA"
                     /locus_tag="ACG8M5_000419"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0357702.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(7491..7868)
                     /locus_tag="ACG8M5_000420"
     CDS             complement(7491..7868)
                     /locus_tag="ACG8M5_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357703.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            8124..8852
                     /gene="purC"
                     /locus_tag="ACG8M5_000421"
     CDS             8124..8852
                     /gene="purC"
                     /locus_tag="ACG8M5_000421"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0357704.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            8852..9106
                     /gene="purS"
                     /locus_tag="ACG8M5_000422"
     CDS             8852..9106
                     /gene="purS"
                     /locus_tag="ACG8M5_000422"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0357705.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            9108..9779
                     /gene="purQ"
                     /locus_tag="ACG8M5_000423"
     CDS             9108..9779
                     /gene="purQ"
                     /locus_tag="ACG8M5_000423"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0357706.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            9780..12002
                     /gene="purL"
                     /locus_tag="ACG8M5_000424"
     CDS             9780..12002
                     /gene="purL"
                     /locus_tag="ACG8M5_000424"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0357707.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            11987..13426
                     /gene="purF"
                     /locus_tag="ACG8M5_000425"
     CDS             11987..13426
                     /gene="purF"
                     /locus_tag="ACG8M5_000425"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0357708.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            13449..14501
                     /gene="purM"
                     /locus_tag="ACG8M5_000426"
     CDS             13449..14501
                     /gene="purM"
                     /locus_tag="ACG8M5_000426"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0357709.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            14498..15076
                     /gene="purN"
                     /locus_tag="ACG8M5_000427"
     CDS             14498..15076
                     /gene="purN"
                     /locus_tag="ACG8M5_000427"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0357710.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            15073..16608
                     /gene="purH"
                     /locus_tag="ACG8M5_000428"
     CDS             15073..16608
                     /gene="purH"
                     /locus_tag="ACG8M5_000428"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0357711.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            16624..17898
                     /gene="purD"
                     /locus_tag="ACG8M5_000429"
     CDS             16624..17898
                     /gene="purD"
                     /locus_tag="ACG8M5_000429"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0357712.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            18020..18934
                     /locus_tag="ACG8M5_000430"
     CDS             18020..18934
                     /locus_tag="ACG8M5_000430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0357713.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(19067..20632)
                     /locus_tag="ACG8M5_000431"
     CDS             complement(19067..20632)
                     /locus_tag="ACG8M5_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357714.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(20625..22076)
                     /locus_tag="ACG8M5_000432"
     CDS             complement(20625..22076)
                     /locus_tag="ACG8M5_000432"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0357715.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(22090..23106)
                     /locus_tag="ACG8M5_000433"
     CDS             complement(22090..23106)
                     /locus_tag="ACG8M5_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0357716.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            23326..24414
                     /gene="chvE"
                     /locus_tag="ACG8M5_000434"
     CDS             23326..24414
                     /gene="chvE"
                     /locus_tag="ACG8M5_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0357717.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            24468..25994
                     /gene="mmsA"
                     /locus_tag="ACG8M5_000435"
     CDS             24468..25994
                     /gene="mmsA"
                     /locus_tag="ACG8M5_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0357718.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            25996..27201
                     /gene="mmsB"
                     /locus_tag="ACG8M5_000436"
     CDS             25996..27201
                     /gene="mmsB"
                     /locus_tag="ACG8M5_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0357719.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            27458..28435
                     /gene="guaC"
                     /locus_tag="ACG8M5_000437"
     CDS             27458..28435
                     /gene="guaC"
                     /locus_tag="ACG8M5_000437"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0357720.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            28699..30123
                     /locus_tag="ACG8M5_000438"
     CDS             28699..30123
                     /locus_tag="ACG8M5_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0357721.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(30165..30422)
                     /locus_tag="ACG8M5_000439"
     CDS             complement(30165..30422)
                     /locus_tag="ACG8M5_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357722.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            30584..31693
                     /gene="pdhA"
                     /locus_tag="ACG8M5_000440"
     CDS             30584..31693
                     /gene="pdhA"
                     /locus_tag="ACG8M5_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0357723.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            31696..32673
                     /locus_tag="ACG8M5_000441"
     CDS             31696..32673
                     /locus_tag="ACG8M5_000441"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0357724.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            32698..34341
                     /locus_tag="ACG8M5_000442"
     CDS             32698..34341
                     /locus_tag="ACG8M5_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0357725.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            34349..35755
                     /gene="lpdA"
                     /locus_tag="ACG8M5_000443"
     CDS             34349..35755
                     /gene="lpdA"
                     /locus_tag="ACG8M5_000443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0357726.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            35917..36192
                     /locus_tag="ACG8M5_000444"
     CDS             35917..36192
                     /locus_tag="ACG8M5_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0357727.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            36198..36965
                     /locus_tag="ACG8M5_000445"
     CDS             36198..36965
                     /locus_tag="ACG8M5_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0357728.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            37133..38962
                     /gene="typA"
                     /locus_tag="ACG8M5_000446"
     CDS             37133..38962
                     /gene="typA"
                     /locus_tag="ACG8M5_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0357729.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            39223..40995
                     /gene="recQ"
                     /locus_tag="ACG8M5_000447"
     CDS             39223..40995
                     /gene="recQ"
                     /locus_tag="ACG8M5_000447"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0357730.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            41109..41411
                     /locus_tag="ACG8M5_000448"
     CDS             41109..41411
                     /locus_tag="ACG8M5_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0357731.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            41414..42577
                     /locus_tag="ACG8M5_000449"
     CDS             41414..42577
                     /locus_tag="ACG8M5_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0357732.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            42603..46031
                     /locus_tag="ACG8M5_000450"
     CDS             42603..46031
                     /locus_tag="ACG8M5_000450"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0357733.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            46052..47176
                     /locus_tag="ACG8M5_000451"
     CDS             46052..47176
                     /locus_tag="ACG8M5_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0357734.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            47157..47603
                     /locus_tag="ACG8M5_000452"
     CDS             47157..47603
                     /locus_tag="ACG8M5_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0357735.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            47587..47925
                     /locus_tag="ACG8M5_000453"
     CDS             47587..47925
                     /locus_tag="ACG8M5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0357736.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            48044..48604
                     /gene="rsmD"
                     /locus_tag="ACG8M5_000454"
     CDS             48044..48604
                     /gene="rsmD"
                     /locus_tag="ACG8M5_000454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0357737.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            48601..49092
                     /gene="coaD"
                     /locus_tag="ACG8M5_000455"
     CDS             48601..49092
                     /gene="coaD"
                     /locus_tag="ACG8M5_000455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0357738.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            49082..50125
                     /locus_tag="ACG8M5_000456"
     CDS             49082..50125
                     /locus_tag="ACG8M5_000456"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0357739.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            50284..51012
                     /locus_tag="ACG8M5_000457"
     CDS             50284..51012
                     /locus_tag="ACG8M5_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0357740.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            51128..51373
                     /locus_tag="ACG8M5_000458"
     CDS             51128..51373
                     /locus_tag="ACG8M5_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0357741.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            51370..51588
                     /locus_tag="ACG8M5_000459"
     CDS             51370..51588
                     /locus_tag="ACG8M5_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0357742.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            51724..52263
                     /locus_tag="ACG8M5_000460"
     CDS             51724..52263
                     /locus_tag="ACG8M5_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0357743.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            52256..54529
                     /locus_tag="ACG8M5_000461"
     CDS             52256..54529
                     /locus_tag="ACG8M5_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0357744.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            54567..55613
                     /gene="holA"
                     /locus_tag="ACG8M5_000462"
     CDS             54567..55613
                     /gene="holA"
                     /locus_tag="ACG8M5_000462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0357745.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(55702..55956)
                     /gene="rpsT"
                     /locus_tag="ACG8M5_000463"
     CDS             complement(55702..55956)
                     /gene="rpsT"
                     /locus_tag="ACG8M5_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0357746.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(56356..56625)
                     /locus_tag="ACG8M5_000464"
     CDS             complement(56356..56625)
                     /locus_tag="ACG8M5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357747.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(56887..57015)
                     /locus_tag="ACG8M5_000465"
     CDS             complement(56887..57015)
                     /locus_tag="ACG8M5_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357748.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(57095..57550)
                     /locus_tag="ACG8M5_000466"
     CDS             complement(57095..57550)
                     /locus_tag="ACG8M5_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0357749.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(57688..59616)
                     /locus_tag="ACG8M5_000467"
     CDS             complement(57688..59616)
                     /locus_tag="ACG8M5_000467"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0357750.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(59813..60646)
                     /locus_tag="ACG8M5_000468"
     CDS             complement(59813..60646)
                     /locus_tag="ACG8M5_000468"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0357751.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(60838..61542)
                     /locus_tag="ACG8M5_000469"
     CDS             complement(60838..61542)
                     /locus_tag="ACG8M5_000469"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0357752.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(61565..62191)
                     /locus_tag="ACG8M5_000470"
     CDS             complement(61565..62191)
                     /locus_tag="ACG8M5_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0357753.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            62517..64892
                     /locus_tag="ACG8M5_000471"
     CDS             62517..64892
                     /locus_tag="ACG8M5_000471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0357754.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(64916..65407)
                     /locus_tag="ACG8M5_000472"
     CDS             complement(64916..65407)
                     /locus_tag="ACG8M5_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0357755.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            65594..66514
                     /locus_tag="ACG8M5_000473"
     CDS             65594..66514
                     /locus_tag="ACG8M5_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0357756.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            66668..67012
                     /locus_tag="ACG8M5_000474"
     CDS             66668..67012
                     /locus_tag="ACG8M5_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0357757.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(67202..67702)
                     /locus_tag="ACG8M5_000475"
     CDS             complement(67202..67702)
                     /locus_tag="ACG8M5_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0357758.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            68230..68313
                     /locus_tag="ACG8M5_000476"
     tRNA            68230..68313
                     /locus_tag="ACG8M5_000476"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68264..68266,aa:Leu,seq:cag)
     gene            complement(68637..>69071)
                     /locus_tag="ACG8M5_000477"
     CDS             complement(68637..>69071)
                     /locus_tag="ACG8M5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0357759.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        22364 a        12054 c        14854 g        19799 t
ORIGIN      
        1 atatatcgtc ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag
       61 taaaagattg gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta
      121 acagaaacat agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag
      181 gagcaaataa agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg
      241 agggcgtatt aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac
      301 aaatgggaaa acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca
      361 ttgaagcttc tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga
      421 tcttcgctag aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt
      481 attcttttac gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag
      541 aagacaaagt attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga
      601 tcgaattatg ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt
      661 ctttccaaga tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc
      721 gtattgcttc cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga
      781 aaaagaaaca caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta
      841 tgcaggagca gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca
      901 gatgacttat ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt
      961 gacagttaac cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc
     1021 agtagctcca ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat
     1081 tatcgcttct gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact
     1141 attccctcct atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt
     1201 agcaatcgag aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa
     1261 tctgctactt gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg
     1321 gttcattcgt tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt
     1381 catgggaatg attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc
     1441 cttttatttt ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc
     1501 catggtcagc atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa
     1561 aaatgttgga gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt
     1621 aggcggtatt ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca
     1681 actttcggga atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt
     1741 aggattattc cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg
     1801 aggaatgtta gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt
     1861 tgatttcagc aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg
     1921 tttcaatgtt acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg
     1981 aaatggcatc gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt
     2041 gaaaaaagat gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt
     2101 tgtgaaatca gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta
     2161 ttttgccgtt tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga
     2221 ggtcgtgagc ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc
     2281 atgtttgttg aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca
     2341 taaaaaggaa tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga
     2401 acaataaaat gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt
     2461 gttaaaatat gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga
     2521 ttatggggag tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat
     2581 tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg
     2641 aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag
     2701 ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg
     2761 tccaatcacg aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag
     2821 gagtacctgt agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg
     2881 cgatacagat gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt
     2941 caatgttggc taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct
     3001 ggttcaacga tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca
     3061 aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa
     3121 gttgcagatt ggcacattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc
     3181 agaagaacag atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg
     3241 attttaccca tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc
     3301 ctgttagaaa aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc
     3361 gtcagtccga cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa
     3421 accactcgag gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta
     3481 gctcctgcca tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt
     3541 gaaaaagaga tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg
     3601 gtcgtggaaa acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt
     3661 gatcaggatg tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc
     3721 ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat
     3781 gaattagctc ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt
     3841 cagttcgatg cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg
     3901 ataagcaatg cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg
     3961 attgagaata agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt
     4021 cgcaagatgg gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc
     4081 aaacaaacga atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct
     4141 gaaatgggga atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt
     4201 ttagcagatg aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt
     4261 cgtgagcatg catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat
     4321 gatgtcgttg cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc
     4381 cattatggtc taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa
     4441 gcaaatgata ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca
     4501 aaagaacaca aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact
     4561 acttttggat tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt
     4621 gaacatgcgg caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca
     4681 aatatcccgc catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa
     4741 atctctactc aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt
     4801 attgctacaa gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca
     4861 cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa
     4921 aggaatccga tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg
     4981 gtcactgcct atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca
     5041 gaacgaatca tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc
     5101 aatatcgtta aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca
     5161 tttggtttga tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga
     5221 gaagaagctt atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag
     5281 ttccgcccgc tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata
     5341 gacgatgcct ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta
     5401 ggtttggaat aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataat
     5461 cgagtagagg ggaaagccaa ctgaccttcc gcccctctca caccaccgta cgtacggacc
     5521 cgtatacggc ggttcaataa gttgagtgta cgaactggta cttcaaggag atgtcctcat
     5581 atccttggcg ggccagttct ttgtctgtta atgatcgatg aagaatatga cttttggaga
     5641 ctgaccaaat accttttctt gtatttgacc attcatacgc tttcgcttta tcgatcccta
     5701 aacgaatcag attttttctt ttggttcttg gattcttcca ttgtttccaa atatattgtc
     5761 tgattcttcg cttcaaccaa ccgtttaact ccctcatgaa ttctttcatc tcaccaattc
     5821 catagtagtt gatccaacca gtcattagtt gcttgatttc ttttaaaata agttggatac
     5881 ttcttccacg gtttctttta gtgagttgtt tgagcttgct tttcacacgc tgttttgctt
     5941 ttgcgtgagg gcgaatttta atacctttct ttgttggcag aatgcagaat ccaagaaact
     6001 ttcgtttcaa tggacttcct accgcactct tttctcgatt taccgtcaac tttagttgtt
     6061 cttcgagaaa ttgaatggat ttcttcatca cacgctgtcc tgctcgtttg cttctaacat
     6121 agatattgca gtcatcggcg tatcgaacaa atttatgtcc tagattttct agcacttgat
     6181 caaattcgtt caggtagatg ttacttaaca ggggagagag attgcctcct tggggcgttc
     6241 cttcttcagt tgttgtgatg agtccatttt ccattacacc actcattaag tattgacgga
     6301 tcagttttag gacttgtttg tcctttactt tccgctcaat gaaatacatc agcttatcgt
     6361 gatttacggt gtcaaagtag gctttcatat caatgtccac cacatatcgg taaccttgtt
     6421 cataataaga ttttgctttc ttcactgcgt catgtgcgct gcgatttggg cgaaacccat
     6481 aactattatc tgagaattct ggttcaaata gcggttgtaa cacttgattg attgcctgtt
     6541 gaagcatacg atcaataacc gtgggaattc ctagttttct tacaccgccg tttggtttcg
     6601 gtatttctac tcgcaaaaca ggctgaggtt tatagttccc ttggcgtaaa ttggatagta
     6661 attcctcgcg gtgctctttc aagtaaggaa gcagttgatc gacggtcatc ccatctgcac
     6721 cagccgcccc tttatttctg actacttgaa gatacgcttg attgagatta ttccgatcta
     6781 gaattctccc aaacagattt tctacaccat cttgtttcct ctgttcaccg caagaagtgc
     6841 tgcgcacttc atcataccct tcgtgttcca cactagctct ttgatgatag ccaagttttc
     6901 ttgttttctg cggtcgtcgc atcttctcac acctcctaaa ttattcaaga ttcttattgt
     6961 tcagtccttc gtaacggtca gtgtcgttac tactatgacc tcggctgact tctcatgatt
     7021 caccatcatg tcgccatgat cgttgtgctg gtttgttttc attcaacgcc cacaacaccc
     7081 tcatgagatc tcccccggta agagtgaaaa ctttcctccc atgtaactgc tagatttact
     7141 gtataggttt cgggcagtat tggactttgt cttgtgatga agacttatcc gacctaattc
     7201 agccttgtat ctagtttctg ttcgtcagtt caggagtttg cgtccgactt ccttcagcca
     7261 caacctcacg attgccgcct tgtctttcgc taacagttcc tactgccaag cctgtagtgg
     7321 actttcacca ccaagttttc acccatgcag ggcgcacacc aaaaaagaag cagatccgtt
     7381 agcggttctg cttcttttag tttattatat tttcttatca ataataggaa cctgatttta
     7441 aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa
     7501 atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa gttttcgaga
     7561 aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg atttttgaaa
     7621 cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt ctgataaaga
     7681 aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt tcggaatgta
     7741 taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa atcattatat
     7801 agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg ttttattgca
     7861 tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta attctaatat
     7921 accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg tttctgataa
     7981 aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg atttataaaa
     8041 agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat
     8101 gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa ggaaaagcca
     8161 aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta gatcaagcaa
     8221 ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg aataatcaaa
     8281 tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat tttattgaac
     8341 aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg gaagtggtta
     8401 tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat
     8461 tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat ccatttatca
     8521 atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt gagctgaaag
     8581 cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc ggtttggttt
     8641 tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt gcggatgaag
     8701 taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg gacaaagacg
     8761 tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat aaccgtctat
     8821 cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat atgtaaccta
     8881 taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg
     8941 atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac ttgctgatgc
     9001 agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg caaatgtaaa
     9061 tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg
     9121 tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt cgcgagatca
     9181 tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga tttgatgggg
     9241 ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg attgctcggt
     9301 tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca gtattcggta
     9361 catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta ctgagacgaa
     9421 atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc aagacggcct
     9481 ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat ggggaaggaa
     9541 attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag attgccttta
     9601 cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg aacaaagcag
     9661 ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg ctaggatcag
     9721 aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg accgtataga
     9781 tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc tatgccgaat
     9841 ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga agaatgccta
     9901 attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc tataaaaatt
     9961 ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa ggccctggag
    10021 aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc
    10081 acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt ggcggaatca
    10141 tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca ttgaggttcg
    10201 gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca ggtatcggcg
    10261 gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt gatccttgtt
    10321 atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac aaacatatcc
    10381 agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc
    10441 gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa gaagaacagc
    10501 aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt
    10561 tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg ggagcagcag
    10621 gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt atattgacat
    10681 tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg ttatcggaat
    10741 cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc caagaactat
    10801 ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac gggcaatatc
    10861 ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta gctgaagatg
    10921 caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt cagcagttag
    10981 ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg ctgcagcaac
    11041 cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta caaaccaata
    11101 cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg aataaagcgt
    11161 tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa atcggaggac
    11221 aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag cccttggcga
    11281 tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg gaattatcga
    11341 cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa
    11401 atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca atgattggta
    11461 tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa aaatcagggg
    11521 atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa ttacaaaaga
    11581 tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt gaaaagagaa
    11641 accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct catgactgtt
    11701 cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag ctagggatat
    11761 ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca agatttgtgt
    11821 tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat gctgctgttt
    11881 tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac ggtcagatca
    11941 aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt cttatgaagt
    12001 gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag acgccgctag
    12061 tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg caggtatcgt
    12121 gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt ctgaggtatt
    12181 caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac atgtacgtta
    12241 tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca aatttcatga
    12301 tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc ttagaaaaga
    12361 gttagaggaa aatggggcga tcttccattc taactccgat acggagatat tgatgcattt
    12421 gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt tgaatgaagt
    12481 caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg ctttggaccc
    12541 aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg tgattgcctc
    12601 ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc agcctggtga
    12661 gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg acacgcagct
    12721 ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca tcgcagggat
    12781 caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat cgccagtaaa
    12841 agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg gttatgcgga
    12901 agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg cccgaacatt
    12961 tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat ctgctgtacg
    13021 aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc ggggtacgac
    13081 aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc atgtgaggat
    13141 tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa cgcgtaaaga
    13201 actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg cagattcttt
    13261 aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag atgctccgta
    13321 ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat atgactatga
    13381 agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa
    13441 gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg
    13501 gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga
    13561 tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc
    13621 cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca atcgaagcag
    13681 acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat atcttagcac
    13741 aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat gatccgttac
    13801 gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat atggcgctga
    13861 ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat gatctagccg
    13921 gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg atcaacccag
    13981 gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac tctcttgttc
    14041 gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca gaattgaaaa
    14101 aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc
    14161 tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg tttgtagaaa
    14221 atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc tcatggccga
    14281 ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg gagatgtatg
    14341 aaatcttcaa tatgggcatc ggtatggtcc tggcagtcca agaatcgaaa gtaaaagaaa
    14401 tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca
    14461 aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg tttttgcttc
    14521 tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag gattggaatc
    14581 ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc
    14641 gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa aagagtatga
    14701 agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat tagctggata
    14761 tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca tcaatatcca
    14821 tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct ttgaagctgg
    14881 agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata ctggaccaat
    14941 catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt tagaagaaaa
    15001 aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg
    15061 aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg gattgtagca
    15121 ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg aacaaagcat
    15181 gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa
    15241 atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct tttaggaaga
    15301 cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc aatcgacttt
    15361 gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt cgaaatagca
    15421 gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac
    15481 catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact ttcagaatta
    15541 cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa agtctttcgt
    15601 catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt ggaagaaaaa
    15661 gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat
    15721 agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc gatcgcagca
    15781 gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc agatgctgcg
    15841 ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca catgaatcct
    15901 tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta tgaagcagat
    15961 cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct gccaacagca
    16021 gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac acaagaagct
    16081 ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt tgcgcaggaa
    16141 gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt acaagatcaa
    16201 gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa gccgactcca
    16261 gcagaagcaa aagcattagc gtttgcttgg agagctgtca aacatgtgaa atctaatgcg
    16321 atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat gaatcgagtc
    16381 ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc agttttagct
    16441 agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca tggtataaaa
    16501 gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat ggcgaataaa
    16561 tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag aggagtcgga
    16621 taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt
    16681 ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa
    16741 gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat ccattttgtt
    16801 aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc
    16861 gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc agcagctctg
    16921 atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat cccgacagct
    16981 aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg
    17041 ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt ggctaccaca
    17101 gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt tggggatagt
    17161 agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct tttggcgttt
    17221 gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg ggcatttgat
    17281 aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc gcaaatttca
    17341 aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa
    17401 gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac gaaggaagga
    17461 ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct
    17521 cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg tacagtagag
    17581 ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc cggttacccc
    17641 gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct ttctgtttac
    17701 tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg tctttattta
    17761 gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg
    17821 gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc tgccaatagc
    17881 gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca aacataatcg
    17941 gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt gttgtgtata
    18001 taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc agaaagcagc
    18061 tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa aagtgattga
    18121 gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg gaaacggtga
    18181 atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag aagatctttt
    18241 tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta aaaaacatat
    18301 cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg atgctttact
    18361 tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt ttgatgaatt
    18421 ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga
    18481 attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat ttggcttgaa
    18541 acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag caagtatcga
    18601 tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc aagcatggtc
    18661 accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt
    18721 aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg gcttggcttc
    18781 tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga tgaacgtagg
    18841 gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag attggtacga
    18901 attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag gtcaaaaggg
    18961 ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga tattctgtct
    19021 ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag ttatttttgt
    19081 ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt ttcttaaata
    19141 aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct gaaatacgat
    19201 agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc agataatcga
    19261 tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa
    19321 cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct ttgataaaat
    19381 ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag ggcagagcag
    19441 ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg
    19501 ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc ttttctttca
    19561 ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt gttcgagttg
    19621 gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt ttcgataatg
    19681 aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt ttcgcttctt
    19741 gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt tcaagtatca
    19801 tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg
    19861 cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt tgggctacct
    19921 gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc
    19981 gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac ttcaaataat
    20041 cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct aataatacgg
    20101 tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat gtttcactga
    20161 tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa
    20221 gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact gctttttcta
    20281 ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct gcctgtacct
    20341 ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg agacgagtcc
    20401 aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga
    20461 tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc atctcaccat
    20521 ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt atttgttccc
    20581 gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac atgtttgttt
    20641 cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc ttctcggtat
    20701 ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag acgcttgttt
    20761 acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc taactcttta
    20821 cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac tttgaattgg
    20881 atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg gacgtacttc
    20941 gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca
    21001 ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta tcttattttt
    21061 aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat ccagtcttgt
    21121 cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt cagagcttct
    21181 tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata aataaaatgg
    21241 ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta ttgtttcaaa
    21301 gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct agcggtaagt
    21361 gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc tgaagaaagt
    21421 tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc taaaaagcga
    21481 cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac tattgttaat
    21541 agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat ctccacacga
    21601 acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg attgatgatc
    21661 tcttcattgg tatccataaa atcttttgcc aagatattat tgatcagctc gttttgataa
    21721 tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc tttaagtgta
    21781 tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat attttcctca
    21841 ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc caaaactttt
    21901 tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt ctgttgataa
    21961 atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac agcaaataga
    22021 agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata
    22081 cttctccagt caataatttc tggttgggat gttagctata ttctgctggg aaaagacagt
    22141 ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc caaaataacg
    22201 agtaacggta ttggctacat accagccgac atcaggttta cactcgacta cacaattgac
    22261 gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta caatgatcaa
    22321 atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt gagtcatttc
    22381 atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta aatatcgttt
    22441 catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa caatagtcat
    22501 cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat tgattgattc
    22561 caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag caagttctag
    22621 gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata aacccgcccg
    22681 atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg gatcattcgt
    22741 ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt cttttaatac
    22801 agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt tataggcaat
    22861 gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc ggtagagaac
    22921 ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt tccgccagct
    22981 actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt cctttgtatt
    23041 aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg
    23101 tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat aaaagggaaa
    23161 atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa
    23221 aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc
    23281 tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa aaacaaattt
    23341 agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag tgcgtgtggc
    23401 aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca
    23461 gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa actgggttat
    23521 aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca aatagaaaat
    23581 atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc ggctttaact
    23641 gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga tcgtttactg
    23701 atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt aggtgtatcg
    23761 caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc attcacgatt
    23821 gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa cggagtgatg
    23881 tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg acagacgagt
    23941 ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag gatggataat
    24001 ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca
    24061 ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga aagtaaacct
    24121 ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat cattgccggg
    24181 gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac gattgaaatg
    24241 atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac ttatgataat
    24301 ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa agataattat
    24361 caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa ttaagaggaa
    24421 ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg gcggattgta
    24481 ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta gattgcgtga
    24541 atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat
    24601 caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac ggggaaatct
    24661 tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa aaaggcatcg
    24721 tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag aatatttttc
    24781 tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa aagaaaacat
    24841 atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta tcccagattg
    24901 gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt
    24961 tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat ttgctgagat
    25021 tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat aagctgaatg
    25081 aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg attgagacac
    25141 tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc agagatttag
    25201 ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa gtagaagact
    25261 ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc aacttgcatt
    25321 tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga actgaattcg
    25381 ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata ttaaaggaag
    25441 gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc gcttatgtct
    25501 cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa aacgtgacgc
    25561 tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa gaagtaactg
    25621 aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat caaattgtca
    25681 gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg atgacacaac
    25741 ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca aaatatgaga
    25801 tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt atctcttctg
    25861 aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa ggacgtatta
    25921 caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta atgacgaaag
    25981 aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta
    26041 aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat cattttagcg
    26101 ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc attgaatata
    26161 actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat gttattagtt
    26221 gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt
    26281 gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat cgcatgtctt
    26341 gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc agggataccg
    26401 gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca agttgttcta
    26461 ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac gggttattta
    26521 ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg aatgatcatc
    26581 acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa tcatttattt
    26641 gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt cgttgtacta
    26701 ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat cattcttggt
    26761 ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg acagatttat
    26821 gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg
    26881 ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt tttagctgct
    26941 cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc aatggcggca
    27001 gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg tgcgattgtc
    27061 ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt cggggtagac
    27121 tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga cttgtataat
    27181 aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact gtgacaagac
    27241 atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt gtatagacta
    27301 aaaataatat atgttttttt accattcgta taaattctct ttttgctctt gatttttttg
    27361 cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt aataatgttc
    27421 gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga
    27481 tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg atacaacggt
    27541 cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat
    27601 tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt
    27661 tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc
    27721 gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc
    27781 gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact cagttattga
    27841 tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg
    27901 tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat
    27961 tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca
    28021 actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg
    28081 gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat
    28141 tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat
    28201 cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt
    28261 tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat
    28321 gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg
    28381 aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat
    28441 aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa
    28501 acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt
    28561 tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt
    28621 ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga aaaaatagac
    28681 aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt
    28741 caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa
    28801 cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac cgttgtccat
    28861 gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag cggatggttt
    28921 gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg gcaaatgatc
    28981 tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc aaagcagatc
    29041 caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct tttgccgaat
    29101 tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga aatggatctt
    29161 ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa atttgaattg
    29221 gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat ctttttgatt
    29281 ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc ttttttttac
    29341 cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat ggatgaagaa
    29401 gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa agcagtgctt
    29461 atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg acgagaaaag
    29521 ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt atttacggat
    29581 ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc ggataatgtg
    29641 ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata
    29701 ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga gttttcgatg
    29761 aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga tttgaaaatg
    29821 caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg gggagattca
    29881 caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat tacttgggat
    29941 gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct tggaattgca
    30001 ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag taggctggat
    30061 gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg gttaacagaa
    30121 taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat ttcaagctgt
    30181 ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc attatttgta
    30241 cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat aacaccaacg
    30301 tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa
    30361 atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat gcggattggc
    30421 ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat aaatccatga
    30481 taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt tcaatcgcag
    30541 aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa acaaaaaagc
    30601 accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag tatatcaagc
    30661 acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat ccgatgatga
    30721 actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac gttcaacagc
    30781 gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag aggcaagtca
    30841 gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt atcgggatgt
    30901 acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt ctcgaggaca
    30961 tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg
    31021 tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa
    31081 tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat
    31141 caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca acgggtatgc
    31201 gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc
    31261 agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat atactgtatc
    31321 taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg aaacactgac
    31381 ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc gtacaaaaga
    31441 cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat atttgacgga
    31501 aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta aagaagaaat
    31561 caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag atttcttgaa
    31621 aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt atgaagcgaa
    31681 ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga tgccttagca
    31741 cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga
    31801 ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg agtattcgat
    31861 actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt
    31921 cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga cgaagtagta
    31981 gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc catcacaatt
    32041 cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa tttagaaggt
    32101 ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta tgatgcaaaa
    32161 ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga acacatgaaa
    32221 ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc tttggataaa
    32281 gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc aatggtgcgt
    32341 gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga aatcattgat
    32401 cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga aaaaactggt
    32461 cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca agtagtttct
    32521 gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt ttctgcacct
    32581 gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc aaaagatatc
    32641 gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa caaagtcatg
    32701 gcttatcaat ttaaattgcc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    32761 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    32821 gacaaatcag tagaagaaat cccgtcacct gttacaggaa cggttaaaaa tgtgattgtt
    32881 ccagaaggta ccgtagcaaa tgttggggat gtattagtag aaatcgatgc accaggacac
    32941 gaagacaatg aaggggacag tggtgtagcc gcagaatccc aaactccagc taaaccggct
    33001 gctgaaccca ctgttgatac agaatcagct ggatcgtcaa gcgaaggagt cttccaattt
    33061 aagttaccgg atatcggtga aggaatcgca gaaggtgaaa tcgtcaaatg gttcgtcaaa
    33121 ccaggagata cgatcaatga agatgataca ttactagaag ttcaaaatga caaatcagta
    33181 gaagaaattc cgtcacctgt cacagggaca gttaaaaacg tgattgttcc agaaggcact
    33241 gtagcaaatg ttggggatgt attagtagaa atcgatgctc ctggacacaa tagtgcaccg
    33301 tcaactagtg cgccatcagc tgaagcacca aaagaaaaag tggaaacatc aggttcagca
    33361 agtgtcgttg aagctgccga tccaaataaa cgtgtattgg ctatgccatc tgttcgtcaa
    33421 tttgcccgtg aaaaagatgt agatatctca caagtaactg caactggaaa aggtggcaga
    33481 gtaacaaaag aggacatcga aaacttcttg gcaggtggtc caagcagtgc accagcgaaa
    33541 tcagaagcgc cagaagcggc agcaccgaaa gaagcagctc ctgcagcaga aagcaaacca
    33601 gctgcaccag caaaaccatt caagtccaac ttaggagact tggaagaacg tgtagcaatg
    33661 acgccaacac gtaaagctat cgctaaagca atggtcaaca gtaagcatac agctccacac
    33721 gtgacattgc atgatgaagt agaagtgtct aaactatggg ataatcggaa acgatttaaa
    33781 gaagttgctg ctgcaaatgg tacgaaactt actttcttgc catatgtcgt gaaagcttta
    33841 acagcgactg ttaagaaata cccagtattg aatgcatcta tcgacgatgc aaaccaagaa
    33901 atcgtttata aacattacta caatatcggt attgcaactg atacggatca cggactatat
    33961 gtaccaaatg tgaaagatgc agaccgcaaa ggaatgtttg ctattgcaga cgaaatcaat
    34021 gagaaagcaa aattagctca tgacggcaaa ctatctgcag aagatatgcg caatggaaca
    34081 attacgatca gcaatatcgg atctgtaggt ggcggttggt ttacaccagt catcaactat
    34141 ccagaagtag ctattttagg tgtaggaacg attgcacaac aaccaatcgt aaatgctgaa
    34201 ggcgaaatcg ttgtcggtcg tgtgatgaaa ctgtcattaa gtttcgatca ccgtatcgta
    34261 gatggagcaa ctgcgcaaca agcaatgaat aatatcaaac gtcttttagc tgatccagag
    34321 ctattaatga tggaaggatg ataaacaaat ggtagttgga gattttgctg tagaattaga
    34381 tacagttgta attggtgcgg gacctggtgg ctatgtagct gccatccgcg ctgctgaaat
    34441 gggacaaaaa gtagcaatca ttgaaagaga gtatattggc ggtgtttgtt tgaatgtcgg
    34501 ctgtattcct tcaaaagcac tgatagctgc aggacaccat tatcaagagt cactagattc
    34561 tgaaatgttt ggagtaacta gtgaaaacgt caaactagat tttgcaaaaa cacaagaatg
    34621 gaaagaaaac aaagttgtcc atacgttgac ttctggtgtt ggtttcctat taaaaaaaca
    34681 taaggttgaa acaatcgaag gagaagcgtt cttcgtagat gatcacacac tacgcgtcat
    34741 tcatccagat tcagctcaaa cttattcgtt caataacgca atcatcgcaa caggttcccg
    34801 tccaatcgag attccaggat ttaaattcgg aggacgtgtg ttagattcta ctggcggatt
    34861 agcattgaaa gaagtaccta agaaatttgt gatcatcggt ggcggtgtga ttggtgccga
    34921 attgggcgga gcgtacgcaa acttaggagc agaagtaacg attcttgaag gctcaccaca
    34981 aattttacca acttacgaaa aagatctagt aaaattagta gaagatgatt tcaagaaaaa
    35041 aggcgtaaca gtcgtaacga atgcaatggc gaaagaagca gtcgataacg gtgacagcgt
    35101 gacagtgaaa tatgcagtgg acgggaaaga agaatccgtt actgctgatt atgtgatggt
    35161 cactgtaggt cgtcgtccga atacagatga tatgggctta gaacaagcgg gtgtcgaagt
    35221 aggagaacga ggattgatca cggtggataa acaaggacgt acgaacgtac ctaacatcta
    35281 tgccatcggt gatatcgttc caggggcggc attagcacac aaagccagtt atgaagcaaa
    35341 aatcgctgct gaagcaatct ctggcaaaaa agtagctgta gattataaag caatgccggc
    35401 agtagccttc actgatcctg aattggcttc tgtaggtatg acgatcaaag aagcaaaaga
    35461 tgctggtatc gaagcaaaag catataaatt cccattttca gggaacggtc gggcattatc
    35521 gcttggtaaa acagaaggat tcatccgtct agttacgacg atcgaagata acgtgcttat
    35581 tggcgcgcaa atcggtggag tcggcgcaag tgatatggtc tctgaattag ctttagcaat
    35641 cgaatcagga atgaatgctg aagatatcgc cttgacgatt catcctcatc catctcttgg
    35701 cgaaatcgtc atggatgctt cggaattagc attaggttta ccaatccata tttgatttga
    35761 ataatacagg cgaaacgcaa ctgaaaaatc agttgcgttt tgttttttag aattatcaat
    35821 aatattatta tgtaaactta aaaatatgac aacttcttgg tttattacca cttcctatgc
    35881 tacactgaag aggaatatga aataggagag ttgaagatgc aagaatatca gtatccactt
    35941 gatttagatt ggacaacaga cgaaatggtc attgtaatga agatgtggga agatctggaa
    36001 cgtgcgaatg aaaaagggat aaacaatgaa gcttttttga agacttatca gcagtttaaa
    36061 actgttgtca aatccattgg tgaggaaaaa cgattgggac gggaatttga aaaagcttcc
    36121 ggttattctt tatatcgtac agtgcagcaa gcaaaaaaaa atccaaacaa aactttgaga
    36181 atgaacgggt gagataaatg gaacagatga ttgatcatat caagtcctgg cttgaagaag
    36241 ctggtgagaa aatcaaatct tctcaacaaa cattaacaat ctcgcaaaaa tcaaatcgaa
    36301 cggatctagt aacaaatgtc gatcgaaaca tccagaattt tctaatcgaa aaaatccatt
    36361 cctttgatcc agaggcgaaa atattggcag aagaagaagg gtgtaatcaa ttatcggatc
    36421 tcgatggaag agtctttatc atcgatccta ttgatggcac cttgaatttt gtgattgaag
    36481 gggaaaattt ttgtattatg ctcgctgttt atgaatccgg cgttggaaag cttggtttca
    36541 tttatgacgt gatgagaaat gaactttttt ggggtggaaa aggaataggc gtgtataaaa
    36601 atgatgagcc attgcctaga ccagcaaata aatccttatc ggaaggctta cttggtgtga
    36661 atagttatct tttcggtcat aataaatatg gaatccgaga aattgcggaa agcagcatgg
    36721 ggacacgtat gtacggatgc gctggtcttg aactagtggc aatcttgaaa ggaaaccaca
    36781 ttgcttatat ttcaaacctt agcccttggg attatgcagc gggaaatgtc ttattggaag
    36841 agtttggtat ggtaacaagc ggtctttttg gtactccttt gacatttgaa aaaagggaat
    36901 actatttagc agggacttct tgtgcatatg cagaaatcgc agaccttttg aaaaaagaag
    36961 gctgaaaaaa aggtagaaat cattcacaaa aatgataaaa acgagcaaaa gattaatttt
    37021 ctcgaaaaaa tgaaaacatt ttactatatt tcatgaaaaa agtctgctat aattagcagt
    37081 cgagtaaaat ttgccctgtc ataaagaacc aataaaaaag gagttagtta cattgaatta
    37141 tagaaacgat atccgtaacg tcgcaattat tgcccacgtt gaccacggaa aaacaacatt
    37201 agttgatgaa ctattaaaac aatcagatac attagacgca catacgcaat tgcaagaacg
    37261 tgcgatggac tcaaatgcgc tagaaaaaga acgtggtatc acgattttag ccaaaaacac
    37321 agctgtagac tataaaggta ttcgcgtaaa catcatggat actccaggac acgcggactt
    37381 cggtggcgaa gtagaacgga tcatgaaaat ggttgacggc gttgtgttgg tcgtagatgc
    37441 gtatgaagga acaatgcctc aaacacgttt tgtattgaaa aaagcactgg aacaacatat
    37501 cacaccaatc gttgtcgtaa acaagattga caaaccttct gctcgtcctg agcatgtcgt
    37561 agatgaagtt cttgaactat tcatcgaatt aggagcagat gatgatcagt tagactttcc
    37621 tgtgatttat gcatctgcac taaatggtac gtcaagcttg tcagatgatc cagcagatca
    37681 agaaccaacc atggcaccta tttttgatac aattattgaa aaaatccctg ctcctgtcga
    37741 taacagcgac gaaccattac aattccaagt ttcattactt gactacaacg attatgtagg
    37801 tcgtatcgga atcggacgtg tatttagagg aacaatcaaa gtaggagacc aagtagcatt
    37861 aattaaatta gacggaacag ttaagaaatt ccgtgtaacg aaattatttg gtttctttgg
    37921 actaaaacgt ctagagattc aagaagcaaa agctggagac ttgattgctg tctcaggtat
    37981 ggaagatatc tttgtcggtg aaacagttac tcctgtggat catcaagatg cgttacctat
    38041 ccttcacatt gacgaaccaa cattgcaaat gactttctta gttaataatt caccatttgc
    38101 cggtcgagaa ggtaaattcg taaccgctcg taagattgaa gaacgtttga tggcagaact
    38161 tcaaacagat gtatccttac gagttgaacc aacaaactct ccagatgcat ggacagtttc
    38221 tggtcgtgga gaattgcatt tgtctatctt gattgaaaac atgcgccgtg agggatacga
    38281 attgcaagta tctcgtccag aagttattga aaaagaaatc gacggtgtaa aatgcgaacc
    38341 gtttgaacgc gtgcaaatcg atacaccaga agaatatatg ggtagtgtta tcgagtcctt
    38401 gagcttacgt aaaggtgaaa tgcaagatat ggtccataca ggaaacggac aaatccgttt
    38461 gacattctta acacctgccc gcggtttgat tggttattca actgaattcc tttcaatgac
    38521 acgtggttac ggaatcatga accatacttt tgaccaatat ctgccaatgc ttcctggtca
    38581 aattggcgga cgtcaccaag gggcgcttgt ttcgatcgat acaggaaaag caacgactta
    38641 ttctatcatg agtatcgaag aacgcggtac agtcttcgta gaaccaggta cagaagtata
    38701 tgaaggaatg attatcgggg aaaacagccg tgataatgat ttgactgtca acatcacgaa
    38761 agcaaaacaa atgacaaacg tacggtctgc tactaaagac caaacatctg tcatcaaaaa
    38821 accaaaacaa ttgacgttgg aagaatcttt ggaattcttg aatgatgatg aatattgcga
    38881 agtaacacca gaaagcattc gcttgagaaa acaaatttta gagaaaaatg ctcgtgaaaa
    38941 agcatctaaa aagaaaaaat aatctgtaaa aggatgaaga gagaaaccag cttgttataa
    39001 aaaatagctg gtttcttttt ttgtccaact cttttctgtg gagaagtcat ctttgtcaat
    39061 ggacataaat tcagaaaata cgccgaaaga cgtttaaaag acttttaaaa aacttatttc
    39121 tagagagttt ttgtttcata ataagataat gtttcacgaa aggagatcaa cttgtccttc
    39181 gaagaaaatc gaagtaaaat ggcaagagaa aggagcggga agatgaacga cttattaaaa
    39241 caatactttg gctatgatga gtttagacct ggtcaaaaag aaattatcca gaaagtcatt
    39301 gatcaggaaa atgtattagg aatcatgcct accggaagtg ggaaatcaat ctgttaccag
    39361 cttccagctt tactattaga tgggcttact gtggttgtgt cccctttaat ctctttgatg
    39421 aaggatcagg tggacgcggc gaatcaatta ggtatacccg ccacttttat caatagctcg
    39481 ttagatggtt acgaaacagc tagacgcttc caagaaatcg atagacagca gtaccgttta
    39541 ctttatatcg ctcctgaacg ttttatcatg ccggatttta tccaagcaat gaagcgatgg
    39601 aacgtccgta tgatcgctat tgacgaggca cattgtatct ctcagtgggg gcatgatttc
    39661 cgcccaagtt atttacagat ggcgaaccag ctagatcaac tgccaaatcg tcctgtaatt
    39721 gttgctttga cagctacagc gactgtgcaa gtagctgctg acataaaaag acttttaaaa
    39781 attccagaaa acaaccatat tcagactggt tttgaacgag aaaatctacg atttcaagta
    39841 attaaagatc aaaaaaaaga acaatatcta atcgaatatt taaagatcaa caaaaatcaa
    39901 tctggtatca tttatgcagc aacgagaaaa gaagtagacc gtctctatca tttgcttaaa
    39961 aaattcgatt tttccgtggg tagatatcat ggtggtctaa atgagaatga gcgaacagaa
    40021 atgcaagaag cctttttata cgatcgcttg cagctgattg tagcgacaaa tgcatttggt
    40081 atgggtataa ataaaagcaa tgtccgtttt gttatccatt atcaaattcc tggctctcta
    40141 gaagcatact atcaggaagc tggtcgggcg ggaagagatg gtctttccag tgaagccatc
    40201 ctgcttttct ctcctcaaga tatccaagtc caaaagtttt ttatccagca gtcccaaaga
    40261 gaagaagggc aaaagcaaaa agaatatgaa aaattaaaag cgatgacaga atacgtgtat
    40321 attgagtcct gtcttcagca atacatccta aactattttg gcgagacaag cagtccgtgc
    40381 aaccgttgtg gtaactgttt agatgacaga gaaatagtag aagtaacgac agaagcacag
    40441 atggttcttt cttgcttaaa acgtatgggt gagaactatg gtaaacaaat gctgatgaaa
    40501 gtcttagcag gatcgaaaga acaaaagctg caagctttag gttttggaca tttatctacc
    40561 tatggactga tgaaaaacca atcgcaaaaa gaaacgatgc agttaattga atatttgata
    40621 tcgaatggct atcttctgac catcaatggt gagtatcctg ttttgaaagt aacagaaaga
    40681 gggatacaag tattgaaagg acaagaatct gtttatcgaa aggaacccaa gaaagtccag
    40741 cagctatcgg atgaagaaac cgatacacta tttgaagtgc tgcgggaact gcgaacggat
    40801 ctagcatcag aagcaggcgt tcccccgtat gtcgtatttt cagattccac attgaaagaa
    40861 atgagccgaa tacgtccatc aagcagattg gagatgcttc agataaaagg agttggccaa
    40921 agcaagcttg ataaatatgg cgaagccttt ttatctcgga taaaaaatgc ggactctaac
    40981 gttctggata agtagaaaga acgaatacgt aaaaatgaat aaaaacaact ggttaaatct
    41041 gttttttttg ctccttatga aaatcaaata tgttataatt aaggaattag aatgggaggt
    41101 aagcacagat ggattctttg tcgaaaaaac ttgcgatcca agtattaaat gatgaagcag
    41161 aacggatcaa acgattaatc cgaaatcaaa acaacagtct ctgtatctca caatgcaaag
    41221 ccttcgaaga agtggtagat acacagatgt acggcttttc ccggcaagtc ggttttgctg
    41281 tccgtatagg agtaatcgga aagaacgagg gccagctgat gttaagtgag ctagaacgag
    41341 aattgaatca gttatatagt gaggtttacc aagaaattta tgataaaaaa gaagttggca
    41401 aggaggaata gacttgccta agaaagtgaa aaaaaggcat ctattagact atagcatctt
    41461 gattccctat ttaattttat gtgtattggg attgatcatg gtatatagtt ctacgtcgta
    41521 tctattatta gaaaacggac aaaatccgtc cgcatcagtc attaaccaga gtattttttg
    41581 ggtgcttagc ttgattgcta tcgcactact ttataaaatg aaaacagatg ttttgaaaaa
    41641 ccagcgtttg atcatggcag ccatcgcagt tttgacgatt ttgctgctga ttgttgtgtt
    41701 ttttggtaag gagatcaatg gggcgaaagg ttggctgcaa attgcaggat tctcgatcca
    41761 acctgccgaa tacctaaaaa tcatttctat ctggtacctg tctctgactt tatctaaaag
    41821 acaaaacagt gtacaaaaag attttcttgg gactgtgaaa cgtccacttg caatggttat
    41881 cggacttact gcattagtcg ctattcttcc cgattttggt aatgcggcag ttatcttttt
    41941 gattattttg gttctgcttt tagccagcgg agtcaattat gtgtatactt tgattgtagg
    42001 agtaggaggc ttttgtctaa gtacttttac gatctggctg atcaatatca caaacggaaa
    42061 gatcttaccc ggacgtttac agtatatcta taatcgattt gctatttacc aaaatccttt
    42121 ctcagacgaa ttaaataaag ggcatcaact agtcaacggc tactatgcca tgttcaacgg
    42181 aggactattt ggcaggggat taggtaacag tatccagaaa aaaggatttt tacaagaagc
    42241 acaaaccgat tttatttatg caatcgtagt ggaagaactg ggagtgatta tggggatttt
    42301 gatattagcg ttgctattct ttatgattgt gcgcattgtc cttgtaggta tccgttcaaa
    42361 agatccgttc aattcgcttt tgtgcattgg gatcggcgca atgtttttga tccaagtatt
    42421 tgtcaatctt ggaggaatta ctggcgttat tcctttgaca gggatcacgt tcccattttt
    42481 aagtcaagga ggctccagtt tgctgatgct ttcgatttgt gtcggctttg tcctcaacat
    42541 cagtgcggat gaaaaacgca agagcttagg tttataataa aaaaagatgt aggagtggtt
    42601 cgatgaaaaa agttttagtt gctaacaggg gtgaaatcgc tgtacgtgta tttcgtgctt
    42661 gtacagaatt aggaattaaa actgttggaa tctatgcaga agaagatgaa tattccgtac
    42721 accgtttcaa agcggatgaa gcatatttag taggacaagg aaaaaaaccg attgatgcgt
    42781 atctagatat tgaagggatc atttctatcg cgaaagaatg cggagctgac gctattcatc
    42841 cgggttacgg tcttttgtcg gaaaacttga attttgcgca acggtgcgaa gaagaaggca
    42901 tcatttttgt tggacccaaa ctgcatcatt tagatatttt tggagataaa atcaaagcaa
    42961 aagctgcagc tatcgaagcg gggattgctt cgattccagg cacagatgga ccaatcgctt
    43021 ctatagacga tgccttggag tttgcgaaac aatacggtta tccgattatg atcaaggcag
    43081 ctctaggcgg cggcggacgc ggcatgcgtg tagctcacga tgaaaagagt gcaagagagg
    43141 gctacgaacg tgcgaaaagc gaagcaaaag cagcctttgg tagtgatgaa gtgtatgttg
    43201 aaaaatacat cgctaatcct aagcatatcg aagtacagat tctcggggat acgcatggaa
    43261 acgtcatcca tctttttgaa agagattgtt ctgttcagcg ccgtcaccaa aaagtagtag
    43321 aagtagcgcc ttgtgtctct atgaatgaac agcaacgtca aaaaatctgc caggcagctg
    43381 tccaattgat gaaatatgtc gggtatgtca acgcaggtac agtagaattt cttgttgaag
    43441 gagatgattt ttactttatc gaagtcaacc ctcgcgtcca agtggaacat acgatcacag
    43501 aaatgattac agatattgat atcgtaacaa cgcaactgtt gattgctcaa ggtttggatc
    43561 tgcataaaga aatcggattg cctcagcaag aaggaattaa attaaatggt tcagccattc
    43621 aatgccgaat cactacagaa gacccgttga ataacttttt accagacaca gggaaaatcg
    43681 atacgtatcg ttcacctggt ggttttggcg tacgtctgga tgtcggaaat gcttacgcgg
    43741 gctatgtagt gacaccgtat tttgattcat tgctagtaaa agtatgtacg catggtgcta
    43801 cctttgaaac agccattcag aaaatggaac gttgcttgaa agaatttaga atccgtggag
    43861 tgaaaacaaa cattccattt atgctcaatg tcatcacaca tcctgaattc caatcaggaa
    43921 atgcaaaaac aaccttcatt gatagtacag caacattatt tgaatttcct cgactacgag
    43981 accgcggaaa caaaacgatg aaatatatcg gtgaaattac tgtgaatggt tttccaggta
    44041 tcgaaagtgg cgaaaaacca ttttatgaag aaccacgtat gcctaaagat ctaatcacaa
    44101 gaactgatta cgtaacggct aagaatgtat tagatgcgaa gggtgccgat gctttggtcg
    44161 aatggatcaa aggacaggaa aatttgttgt tgacggatac gacatttaga gacgcccatc
    44221 aaagtctgct agcaactcgt gttcggacaa aggatttcaa gcagattgca cgtttgactg
    44281 gagaaggtct tcctgaactc ttttcaagtg agatgtgggg gggagcaaca tttgatgtgg
    44341 cttaccgctt tttgaatgaa gatccttggc aacggttgag aaaaattcgt tctttaatgc
    44401 ctaatacgtt gctgcaaatg cttttcagag gttcaaatgc cgttgggtat tctaattatc
    44461 ctgacaatgt attggtggaa tttgtcaaag aagcagcagc gcaaggaatc gatgtatttc
    44521 gaatctttga tagtttgaac tggactccac aaatggaaaa aagtatccaa gctgtacggg
    44581 atactggtaa aatcgcagag gcagctattt gctatactgg cgatatcaac gatccaagtc
    44641 gggcaaaata caatgttcag tattataaag acatggcaaa ggaactagaa caactgggtg
    44701 cgcatatcat tgcaatcaaa gatatggctg ggctgcttaa accgcaggca gcttatcgtt
    44761 tgattagtga attgaaagaa acgacagatc tgccaatcca tctgcatact catgatacca
    44821 gcgggaatgg aatcatcact tattctgctg cttcaaaagc aggagtagac atcgttgacg
    44881 tggcaatgag tgcaatgagt gggaatacta gtcagcctag catgagcagc ttgtattatg
    44941 cactggtcaa tggtccacgg ctgccagaaa tcacgattga aaatgcacag aaattaaacc
    45001 attattggga agatgtgcgc atgtattaca aaccgtttga aaacggcttg aatgctcctg
    45061 aaacagaagt atatatgcat gaaatgccag gaggacagta ttcgaatcta cagcaacagg
    45121 caaaagctgt cggattaggt catcgctggg atgaaatcaa acaaatgtat catactgtca
    45181 acttgatgtt tggcgatatc gttaaagtaa ctccatcatc taaagtagtt ggagatatgg
    45241 cactatttat ggtccaaaat gatcttacag aagaagatat ttatgaaaaa ggcgaaacac
    45301 taagtttccc tgaatctgtt gtgactttct tccaaggaga actagggcaa cccgttggcg
    45361 gatttcctaa aaaattgcaa aagattattc ttaaaggcag acctgcattg aatgaacgtc
    45421 caggtctttt ggctgaatca gtcgacttca acgaagtaaa aaaagaattg gcagaaaaaa
    45481 ttggctatga accaaaacaa gacgaagtat taagctattt gatgtatccg caagtctttt
    45541 tggactatca aaaagcatat aatcaatttg gcgatgttac attactggac acaccaacat
    45601 ttttccaagg tatacgtttg ggtgaaacaa tcaatgtaca aatcgaacgc ggaaaaacgc
    45661 ttatcattcg tttagatgaa atcggagaac ccgatatcga agggaatcgc gtactgttct
    45721 tcaatttgaa tggacaaaga cgtgaaatca ctatcaaaga taattcgatc atcagtacgg
    45781 tccaaacccg attgaaagct gaaccgacaa accgagaaca aattggagca acgatgtctg
    45841 gttctgtttt ggatgtatta gtgaaaaaag gcgataacgt taaaaaaggt gatacgttga
    45901 tgattactga agcgatgaaa atggaaacgg ccatcgaagc acgttttgac ggagaagtcg
    45961 cacatgtata tgtgtcttca ggagacacta tttcgtctgg tgaccttttg attgaagtga
    46021 ctgagaaata ataagcgaaa ggtggggaaa catgaaaaga ctgggtttgt tttggctgat
    46081 tctgctatta gttattgtgg cattctacat ggaaccagtc ttgtttccgc caaaacttga
    46141 agtgaaggaa aactctgatc aacaaatatc aaaccaaatg acaacgtgga aataccagga
    46201 gcttaaagca actggttttt ctacttatat cggaaaaccg atcgatcagc tggaaaagga
    46261 ttttggccaa gcctatgatc gattaactag cggatttggt tttgaaacaa gatattatga
    46321 aaataaagct agtcatctca catttgaagc gaacatccaa gacagcagag tcacaactgt
    46381 taaagtactg caaacagggc ctgctgatat cgcgccgttt tatttaggga tgacgatgca
    46441 agatcttacg aaaatcacta ctatctatac taatttcact tttgagtacc atgatacaga
    46501 agttggaatc gaactaatgg aagaggatat gaattatcgt ccgctaattg cttttgataa
    46561 cgacactttt gctattttat tttttgatca aacaacaaac ggcctatttt cagttgtgta
    46621 tttagataaa gatagtcttt tgaaactatt gccatatcaa gtgtttggtg aaggccttcc
    46681 acattatcaa gcagataaat cggctgattg ggaatcaatc aatcgcgtta aagaacgaaa
    46741 aagtacgact ttattaaata tgcttagaag agaagacgaa cttcccatat acaatcaaac
    46801 acttgccttt caaaaccaca gtcagctgct actacatgat tttttgacaa ggcctgagga
    46861 aatattgtca gaggaacgta tggctgattg gcaaaaatcg ctaggcagtg ctcaaacgac
    46921 agtgaaattc tcactgacga atgatgaatt ggagaagtta atcagtaaac aaaaattaga
    46981 aaatactagt gggctattta ttcatccggc gattgacgct actttttcgt ttttatattg
    47041 gtacagcgat ccttacaacc atgaccgttt catggacccg ggaacggatt ctgtaggcat
    47101 tgctttttct aaagaaaata tgttagtctt attgcaagaa gaaaacggag aaagtagtga
    47161 tagcagtgat cgttaacgaa gagctgtttc aactggagga tcaatgcctc caagtcgctg
    47221 aaatgataaa aaagagtaaa acgatggctt cttatcttga acataaacag tcaatggaag
    47281 aatctgcaaa agtttgccag ttgaaagaag cgttcttgtc taagaaagaa gcatttgaaa
    47341 atgtagcggc atatggtgag tatgcacctg attatcgtga aaaaaaacga gctgttcgaa
    47401 aagcaaaaag acagcttgat atggatgagc atgttgcagc tttccgatat agtgaaacac
    47461 agctgcaaag tctgttggat caaattgtcc aagtacttgc cgaagaagtt tctaatgaaa
    47521 tcaaagtaga tgcaggtagc ccgttttttg aaacgaaaaa acactttgga tgcggaggta
    47581 attgtcatgc aattagacaa tagtgaattt gaaataacaa aacgacgcgg gctgatcgta
    47641 tgggtctaca gcttaaaaca attaaagaat ctgaaaaagt tcggcttgtt gcattatgtc
    47701 tcaagaagaa tgaaatatgt cgtgctttac ttaaatgaag agacattgga agaaacagaa
    47761 gaacgaatcc gtaaattaca ttttgttcga aacgtagaac gttcttaccg accggatatc
    47821 gaaatgaatt ttgccgaaaa aataggaaac aaaacagtat taaaagacga aggtttcgaa
    47881 attgaagagc taaatacaga aatccgatta gctgaacatc tttaaaaatc tcttatgagc
    47941 agtttttaaa taaaaaataa cttggataac gatcgatttt tgatcattgt ccaagtattt
    48001 ttttggtata ctagtaccag taccatttct aaggagtgag tagatgcgga taatatcagg
    48061 aaattatggc gggcgccgtt taaaaagttt agcaggggcg aacaccagac caactacaga
    48121 taaagtaaaa gaatccatat tcaatatgat tggaccatat tttgatggag agacagtatt
    48181 ggatctgttt gcaggaagcg gtggtttagc tatcgaagca gtatctagag gctgcagtca
    48241 tgcggtatgc gtagacaaaa attaccaagc gctaaaaatc atcaaagaaa acatcgaaat
    48301 tacgaaagaa ccagagaaat ttaccacaat aaaacttgat gcggacaaag caatcgaacg
    48361 gctagcagcg gagaaacaac aattcgacta cttgtttctt gatccgccat atgcaaaaca
    48421 aaaaataatc gatcaaatcc ataaaatgga agaactggga cttttttcta aaaccgcagt
    48481 tatcgtatgc gaaacggata aaacagtcga attgccagaa actgtggctg gattccatca
    48541 aataagaaaa cagatttatg gcataacggc cgtaactatc tatagaaaag agggaaatca
    48601 atgaaacgtg cattattccc aggaagtttt gatcctttta caaaaggtca cttagatacg
    48661 gtggaacgtg cggctaaatt attcgatgag gtagtaattg gtgtttttat caatacttca
    48721 aagaaaagtt tgtttccccc agaagaaaga atgacattga tcacaaaagc tgtttctcat
    48781 cttcctaatg taaaagtcat gcatcaagaa aatcaattga cggtagaaac agcaaaagag
    48841 attggtgcag atgcgttgat cagaggcata cgaagtatca aagactttga atatgaacga
    48901 gagattgctc agatgaatca tcatctatat gatgaattgg aaactgtctt cttattagca
    48961 aaaccagaat atagtcacgt gagttccagt attttaaaag aagtactgca ctttggcgga
    49021 gatgtgagca gctacttacc gccagtgatc aacgaagctt tagctggaaa gcgtgaatca
    49081 aatgaaagct aaacaatggt tgaaaacagt actgattatc atatttggaa tgttgatcat
    49141 tggatttgta gtagttccct tgccgtatta tgtagaatca ccgggagcca cgatcgatct
    49201 gaaagaacta attacggtca atgataagga agataaaggt tctgggtctt tctctcttac
    49261 ctctgttggt atcaggcaag ctacaggttt cttagcttta aaagctaaaa tgaccccttt
    49321 tgaagaattg atcagtgaag aagaattgac tggtggggcg acaaatgaag aatataatca
    49381 aatgcaacag tattatatgg attcttccca aaatgcagca atcgaacaag cgttaaaact
    49441 tgcagacaag ccttataaaa tggaattcaa aggcgtatat gtcatgagtg tagagaaaac
    49501 atcgaatttc tttggcaagc tttcagttgg agatacagta acaaaagtag acgggaaaag
    49561 ctttcaaagt acagaagcat ttatggatta tgtaaaaagc caaaaagttg gacaaacaat
    49621 cgaaatcagt tatttgcata atggtgaaga aaaaacagct tctggtgatc tgatcgagtt
    49681 accaacagat aaaaaggcag gaataggtat cacgcttact gaccataccg agatcgaatc
    49741 agatacagat gttcgtatta attctggctc tatcggtggg ccatcagcgg gattgatgtt
    49801 cactttagaa atctatgaac aagtcactgg taaaaacttg cgacatggta agcaaattgc
    49861 aggaactgga acgatcaaca gtgaaggaga agttggccga atcggaggaa tcgataaaaa
    49921 agtggcaagt gcggataaag ctggcgtaga gattttcttt gcaccagatg atacgatttc
    49981 agacgatgtt aagaaagaat atcctgaaat aaaaaccaac tacgaagaag caaaagctgc
    50041 cgctaaaaaa ttgaattcct caatgaaaat cgtaccagta aaaacagtac aagaagcgct
    50101 tgactatcta gagaaaatga aatgaaacta aataattaaa taggggttgt gacacgagtt
    50161 ctgtcacaac ccctcttttt cgtcaaaaat cttcacgata aaaataaaaa caaaagaaaa
    50221 aattgacgta attagatgtt gaagggacga ttttgccaga gctcactgtt tcttcaattt
    50281 taaatgaaag gagtaatgaa tgatttttac tggtcattct ttgaaaaaag aacaatgata
    50341 gaaaaaatca gcaagaaaat actagcttca cccaaattga tcatttggat gtctatgata
    50401 gttcttatct tttgtatcgc aagtggtttt ttattatcaa atttgctatt ttatagagaa
    50461 acagaagaga ctgctgcaaa agaaatactt tcttcgttta gttccagtgg agaagatcca
    50521 tcagctgaag gaaacgatgt agaagaggat gctcgtccaa aggtcatgta caccgatatc
    50581 aaaggaagtg tgaaggagcc tggtatctat tccttttctt cagaagaacg ggtctatgat
    50641 gtactaaagc gtgcaggtgg attattagaa gaagcggata gcgatcgaat caacttttct
    50701 gcgaagatag aagatcagca agtcctatat atccctgctg taggtgaaga gctgccagaa
    50761 catcttgatc agttggcatc ctcggaagga aaacagccta cagcagattc cgagcctccc
    50821 aaaataaata tcaatacagc cactccatca gaacttcagc agattcctgg gatcggatca
    50881 gtcaaagcgc aagaaatcat tcgatttaga gaagaaaacg gatccttcca gaaagtggaa
    50941 gacctgcagg agatttctgg tatcggtgaa aagacagttg aaaaactaaa aaacttcgtt
    51001 acaattaaat agaatgattc aaaatcgact ggcacgagca ataaatggac gctaatggat
    51061 actaagaata atccaattct aagagcttgg caggacgagg aggtagtatt tttggatagg
    51121 aaatagcttg attgttcgca aagaaacatt aaaaaaacca atgctgaatg tctatctcca
    51181 aaataaaata tctgggattc atattatgaa tacggctgtc agcggcaata acagtcaagc
    51241 tctacgagaa cgctttgcca aagatgtact tagctataca gcagataaag tatttattct
    51301 cattgggacg aatgatctag cagagcataa acaactttct aaagaaacgt accaaaaaat
    51361 ctgcagtgga tgatagttca gctgaaaaaa gactatagtt ctgatcatgc tttctttatc
    51421 acgccaccag cagtcgatga gacaaaacaa aaatatcgga ataacaagtt gatcaaagaa
    51481 tatatctctg ttctcacaga agtagcaaaa cgccatgatt gtgaagtatc gatttttata
    51541 cggaatttct tgaacaaatg gattcgaaga atttttataa tgggctaatg gatgacggac
    51601 ttcatttcgg cagtgaaggg taggatcttt tgtcagacag gatgattcgt tatatttaaa
    51661 gaatattttg tttaagcaat gagtactttt taatcaactt gaaaaaacgg aggaatataa
    51721 aacatgttag aaaaatcttc aaaagcatgc gatacgaata aagaaaaagt tactgataag
    51781 tcacataatg aacgcattcc ttgggaccag tactttatgg ctcaggcagt actgttatct
    51841 ttacgtagta cgtgtacacg tttagaagta ggagcgacac tcgttcgaga aaaaaggatc
    51901 attgctggcg gctataatgg agcagtcagt ggagatgtcc actgcataga tgaagggtgt
    51961 tacgtcgtgg atggacactg cttgcgaacg atccatgcag agatgaatgc actcctacag
    52021 tgtgcaaaac ttggtatccc gacggaaggt tcagaaatct atgtcacaca ttttccatgt
    52081 ttggcctgta caaaggcttt gttgcaagct ggtgtgaaag ggatcaatta tctccatgac
    52141 tatcgaaatg atccttatgc ccaagcgttg atcgaacaat taggtgtatc tgtacaccaa
    52201 gttgctttag actcacagca tttttcaaaa cttcaaaatg aattaacagg aaaacatgtc
    52261 tagcgtttta gaaaggcatg ttcgaaatcg gttgattttt ccggcaatta cgtcgatgat
    52321 tatctgtttg ttgtattaca ctgacaattg gggttatcgt ttgcttcttg ccggaatttt
    52381 tttatggttt tcgctaaaag aaggacgttt tatcgcgtac ttgtgtattt tgtgtagcat
    52441 aacggtatcc atttcttata ggctacaaga gtattcattt ttagaggaaa aaacaacatc
    52501 tgcgcaaatt actgtattta cagatacgat tcgtgcaaat ggtaattttt tgacaatgga
    52561 aggaaggtta tcagagaata aacaaaagct ccagctttct tatcaagtaa acacagaagc
    52621 agaattgaaa acatggctta acttccgagg aaagggaata gaattgactg ctgaaggcga
    52681 gttcatctct ccagcaaaaa agcggaatct ttataatttt gaccaagaag cttacttcag
    52741 gaaccataga atttctggac ggttccaaat tcaaaagatt gaacagatca ccatgcaaaa
    52801 tcactggcga aactggcttc agagaaaaag aggatctttt atctcctttg tccaatctga
    52861 atttccgcaa aaaacaagtg tatatattaa ttctttatta tggggctata aagatgcagg
    52921 attcagagaa ccagaagaaa tttttaggga gagtggtttg ctgcatctgt tcagtctatc
    52981 gggtctacac atccaatttt atttagggtg gctgtattat ttgtttcgac gtatcggatg
    53041 gccactacac gtcagtatta ttccgttgag tctactaact atctgttatg tttctattgc
    53101 aggaagttct atttctgttt tgcgtgctag tttgctgttt ttattgcgat tgctagtcaa
    53161 attgctgaat aagcattatt ccaccatgga cttatttgca gttgtctttt ggcttctttt
    53221 actatttgat cctcgatttc tttttcatgc gggtggacag cttagttttt tcatgagttt
    53281 tttgtttatc ctgatttctt ttgatggaga ttggttgaaa aaaacggcct ctcacgtcct
    53341 gtttactatg atcacgatgc cgttgattgt atggcatttt tatgagtggc ctattttagg
    53401 tagtcttttt acattactga ttgcaccggt attcaagttt ctttttcttc ctggtgtgtt
    53461 atttctcaca ctcttcaatc gctggctgcc ggaaacttta cttttgctga tagaagaggg
    53521 attaatctta tttgagcgaa taatcgattt ttcttcaggg tcgtctctca ttataggacg
    53581 tttgcccctt gtgctatctg ggatgtatat tttcggatta ctgatttgta tggataggtt
    53641 gaaagaacaa cctgtgaaac acttgtttta tgttggtctt ttttctttta tttttttgac
    53701 tttgccattt ttacgctttt cttcaacgat cgcttttatc gatgtcggtc aaggagacag
    53761 tatctttttg caaagtcctt ttcgaaaaga aacgattttg atcgatactg gaggtcagtt
    53821 acactttgaa agagaaaagt gggcgcaagg tatgatacgt cctcctgctg aaagaaatat
    53881 cgttccatat ctaaaaggtc aggggataag tgtaatcgat aaactcatct tgacacacga
    53941 cgatacggat catgtgggag aattacccac gttatctcag cattttacta tcaaaaaaat
    54001 ttatatcggt tggggcgctg gacgaaatga gccattacaa aggcatttat ctgaagctca
    54061 aaaaaatggt actgagatca ttgaaatcaa acatggggac aggatcagcg gttattatga
    54121 tctttatgta ttgactcctt ttgaaaaggg agagggaaga aatgaagatt cgattggtct
    54181 atggatggaa tataacaata ggcgcttttt atttttagga gatttgaatc aagcaatgga
    54241 aaaacaattg cttttgattt atcctaatct gaaagccgat gtagtcaaat taggtcatca
    54301 cggtagccgt acgtcaagta acacagattt tctttcccat attgaagcaa aacatgggat
    54361 tatatcctgc ggcgtaaata atcgttatgg tcacccgcac agcgaagtat tggagacact
    54421 agaagaaaat cagataaaaa catggcgaac ggatcaacaa ggaatgatca cctatcgctg
    54481 gcatccagtt tttcatcctc atgggctagt ggaaacaatg atagactagt aattctcatc
    54541 tgctcatggt aggataattt ttgaaagtgg tgagtaattt gagtatacaa gcagagttac
    54601 aaaaaatacg taaagaaaaa cttgcatctt gttacttgat tttaggaaca gaaaagtttc
    54661 tccaagatca agttcggaca gaaattgtca aaaaaatcca attagagggc gaagatgact
    54721 taaatttttt aagttttgac atggaaaatg caagtttaga tgaggtagta gctgaagcag
    54781 agacgttgcc gtttttcgga gatcaacggt tagtattcgt agagaatcct tattttttaa
    54841 caggtgaaaa agtaaacaat ggaatcgaac aaaatactga tttattgacg gcttatttga
    54901 aagaaccatt agaatcgact gtattagtat tctttgcgcc atatgaaaag ttggatgaac
    54961 ggaaaaaagt aacgaaacaa ttgaaaaaaa cagcaattat catcaatgtc caacagttga
    55021 atgaaaaaga agtacgtcaa tatctgatga ataccttaga gaattcctca atcaaaatgg
    55081 aacgaagtgc aattgattta ttcttgaggt tgaccgactt agatttatcc aaaatgatga
    55141 gagagttaca gaaattggtc ctttatggac aaaatcaaca gcaaatcacg gtaagagaag
    55201 tagaagaatt agttcctaaa acgttagaac ataatatttt tgacatgacc caatatattt
    55261 taaaagggaa gacggaacaa gctttgcgtt tatacgaaga tttagttatg caaggggaag
    55321 aaacaatcaa aattaatgcg attttgcttt ctcaattacg tttattttta cagacaaaat
    55381 ttcttgtaaa gattggttat cagcaggcaa atattgctga aacattgaaa atccatccat
    55441 atcgtgtaaa acttgccatg caggaagtaa aaaaattcga tgaacatttg ctggtacagt
    55501 tgtttgatca attagttgag atggattatc aaatcaaaac gggacaaatc gaaaaagaat
    55561 taagttttca actattcgtt ttgcgcacag ggcagttgtt aagaaatcga taagaagaaa
    55621 gacgagggca aagcatgcgg gaaggtcgtt tgtttttgtc ctttttttga caaataaaaa
    55681 aatcggccga agccgattct tttatttagc gatttttttg cttaaacgag atttgtcgcg
    55741 gttagctttg tttttgtgga ttagaccttt tgattcagcc atatcaatag ctttaacagc
    55801 ttctttatat aacgcatcta cgttgtcagc acctgaagct acagcatctt caaatttttt
    55861 gattgcagta cgcatagcgc ttgtttgaga tgaatttttc acattagcgt tttcgcttgt
    55921 acgaacacgt ttgattgcag attcaatgtt tggcattagt ttcacctccg ataacttaag
    55981 ttcttacgcg tgttaccgca taattcaaca ttcattatta tacctaaagg cagcgtgctt
    56041 tgcaatatga acatgacaag tttttttcct taacagtctt ttattaggat tttttttcag
    56101 taaaaacgga tagtacaagc tgaaaaaaat caaaaagacg ctattgtggg aggcaatagc
    56161 gtcaaaccaa actagatttt tatttatcta tttaaacaaa gtttgaatgg gaatgtttgg
    56221 tactaagtta ttatataaca aagagaaccg tttgcaaagt tttttttgaa aagggatacg
    56281 attccctgtt gttctttgtt tttctattaa gaaactgcag ttattttata accagtatcc
    56341 tttgatcagt aaaggctatt ctggtgtacc gattatttta atgtattcat cgatattttt
    56401 tatcaacatt ttttttatga ccaactggtt ccgacggttc catttgccac ttacttccag
    56461 cacatattta tttaggggca aacgtaagat atattttgcc aaatagcgat ctttaagcag
    56521 gccattgatc acacggtcct caagctgcaa gttgatccca gtcagcatca ttggctgttc
    56581 ttgtaaaacg tacgtatcaa ttaaaagacc ttcaaaagtt tccacaagcg tcttcctttc
    56641 ttttcaaagt aacctgaaaa caggcactaa ttcttccatt ttgatattcg ttgaagtgga
    56701 tgcgatcatt ctttcttttg tccactgtaa tctagtataa caaaaaagag cagaagagcc
    56761 aaaaagaaaa aagagcatcg aattctatag gaagtctcat tggcacggcc taaattatta
    56821 caaaacattg attaaaaaaa gcccctgcat cgcaggagca tcccctatat cgttttaata
    56881 ggtcaatcaa aaagcagtat caaacacatt ttgtatcaaa tcaggaaata atagataagc
    56941 taagcccaga atcaaaagta ccgctagaag aaaccagccg attgttccta tgaaatactt
    57001 taatttttcg aacattgtat cacctcgttg tcagtatatc acggatcagt aaattggggg
    57061 tgttaagaat ctcctaaaat agacaaaatt tgttctattt tcctaaagca aatacttgta
    57121 agatacagta agtgatacca gaagcaatga ccggtccggc ggcgattcct cttaagaaga
    57181 caacacctaa aatcgtgccg aaaacaagtg caacagtgat ttgtggatca gaagcaatca
    57241 atcctacacc cttggaagat aagacagcca ctagcccacc gcaaaaaaca gctacccagc
    57301 caatagggga tttaaaggct tctaatagat cacgtagacc gatttctcct gttgcgatag
    57361 ggattagaat agccgctgtg atgatcgtca ccccaatatt gattcctttg ccttgaagcc
    57421 aagggaaaaa ggaacgggag gctggtatga gcttcataat caaaacaaaa gctgccgcta
    57481 tcatcagact ttgattttta gctaaaattg ctacaagtaa aataattcct aaaaaaagcc
    57541 aactttccat gaaatatatt cctcactttc ttcgaaacaa tcataacatg tttttagaaa
    57601 gttacaaaag taggaattca tccttcaaga cctattcata agctgttttt ttcataaaat
    57661 aaacaaaatc ataaaaaaac actttgctta tttgacagaa gacagccgcg ctttgtatag
    57721 tggacaagac ttacattttt ttgaagaatc agaaagatca ggtaaaggtc ttagtaaacg
    57781 caagccattc agaataacca atattgtgct tccttcgtgt cctacaactc caagaggtag
    57841 atttaaaaat tgcaggaagt tagaagcaat caacaagaga ataacaagaa tcgaaaaaat
    57901 aatattttgt ttgatgatcc gccgcatttt taaagatagc cgatgactgt agacaaattt
    57961 atttaagtcg cttttcatta agacgatgtc tgcgatatcc atagcggcat ctgtcccttg
    58021 ccccatagcg acacctaaag aagcggctgc taaagcaggc gcatcattga ctccatcacc
    58081 aaccatcgca tttacttggt aaagttcatt ctcttttttg actagttgtg ttttttcttc
    58141 cggagtacag cttgcatagt aatgttcaat ccctacttgt tcagcaatgg cctttgctgt
    58201 gccagaatgg tcgccggtta tcatacttgt atggatgttt tgggatttaa agtaggaaat
    58261 tacttgttgg gtatttgctt ttggaatatc tagcagacct aaaacagcaa ttaactgctg
    58321 atctttagaa agataaatca cggttttgcc ttgattttcc aaacgttcga tcatttccgc
    58381 aatctctttg ctgattatca tcgtctcagg atcatatgcg tgcttgccaa ctttccattg
    58441 cgcacctaaa taggtagttt gtaatccaaa accagtaatt tcttctattg gtaagtattg
    58501 gatttcttca ggtattgtgc aatcgaaata ttgcaaaatc gcatgagcaa gtggatgcgt
    58561 cgttttttgt tccatagcaa caagaagttg ttgtgccaaa tccttttcat caaagaaaaa
    58621 gctgtcagtt acaactggtt ttcctttagt gagcgttcct gttttatcaa aggcaatcgc
    58681 ttttaattca gaaagctttt ctaaatggat accgcctttg attaaaatcc cattttttgc
    58741 agaatttgaa agagcagcta gagaagctgg tgtagcagat gcgacaagcg cacaaggaga
    58801 agccacaact aataatacca ttcctcgata aaagctttca tcccaactcc aacctaggaa
    58861 aaagtggggg agaaggatca ttagaggaac agcaagcaaa atcaatttta cataattatt
    58921 ttcgattcgt tcgataaatg aagctgtttg agttggcgta ttctgcgctt cttctactaa
    58981 ttggataatc ttagcaaaga cagtttcgga actcgttttt gttactcgaa tcgtgatggg
    59041 atttccttga ttcagcgttc ctgcatacat ctcatcgcct aaatattttt ctacaggcac
    59101 agattcaccg cttaatgcag actcatcgat cgttgcctgt gtgctttcta aaaagccatc
    59161 tatcggaaca gctgctcctt tagcgacaaa taccaagtta tccaccttta gttgagaaac
    59221 atctacttct tgcatttgtc cattttcttt taataattgg gctttttgtg gttgaagatt
    59281 catcaaagca gtaatttctt tttgactttt attagttgtg tattcctcta atgcaccgct
    59341 taaacaaaag atgaatgtca acattgctcc ttcaaagtaa ttaccgatca aacaggcacc
    59401 aatagcagct aaagccatca ataaatccac attcagtgtc cgatcattcc acaattcttt
    59461 caagccttcc gatgtttgtt tatatccacc gatgataatg gcactgatat aaaaaagtgg
    59521 ggtgatggta agagagaatc ggtcgaagat aaaaccgaag atcataaata aaaggcagcc
    59581 gattgttgct gcaaaagctt ttttctgatt agtcatcgtt attagacctc cttgtcaaaa
    59641 caaggatagc ataataaaaa tgtaattgat aataataaca gctaaatgag aacgataatg
    59701 gttatcattt ggtgattaag cttgatgttg tttttattat ttataatgat tattattaaa
    59761 gaaaataaaa aaccagaaca tttctcaaat aagagaagtg ttctggtttt ttctacattg
    59821 caataaacca ataaccaatc aaaatgatac caaggataat acgataccag ccaaaagcgg
    59881 taaagtcgtt tttcttcaaa tagcttagta agaatttgat agctaagacg gaaaccacaa
    59941 aagatacgaa tgttcctaca agaagaacaa cagtttcttc cattttgaat gaattccctt
    60001 cagctaaata tttgacgatc ttcaagccgc ttgcgccaaa catgacagga atccctaaga
    60061 aaaatgaaaa ctctgtagcg acaaaacgcg aagcaccaat caagatagat cctaggatcg
    60121 ttgcgccaga acgggatgtt ccgggaacaa gtgccaagac ttggaataac ccaacaagaa
    60181 gagctgcttg gtacgtaaag tcattcagag aagtccaacg aggtgttttg tttttgttgc
    60241 gtttttcgac aataataaag gcaataccgt aaacgatcaa aacaatggca actggtaaga
    60301 acttgtggaa atttgcttct aaccaatcat ccaaaggcaa accaatgatg atggctggta
    60361 tgcaagcaac gatcacttta aaccaaagtg tccacgtatc ttttttttct accgttgatt
    60421 tacttggaga aaatgggttt aacttgtgaa aataaagaac gacgacagcc aagatcgccc
    60481 ctaattggat aacgacattg aacatcgaca taaattgtga actggcatcc agtttgatga
    60541 attcatctgc taggatcaaa tgaccagtac tactgatggg cagccattcc gttattcctt
    60601 caataatacc gagaatcact gctttgataa tatttgcaaa aaacataaat tccttccttc
    60661 ccaattaaga tacatcttat agtataccgt tattttttga gaaacccaat ctgtttttta
    60721 atattaagaa aaacttgaga aataaagcag ataagtacac gaagagaaaa aagaagacaa
    60781 agccctcgtt cagactttgc cttttatgat tctctggtaa aaattaaaat atccccatta
    60841 tagaaacgag tcgcgactat gtgttttccc gttatcgata taagacgtat cattgatgcc
    60901 gtggatagaa aatcgaccgt ttgcataagt gacctttgtt acgctgccat ttaaaagccc
    60961 agcacgaaca ggctgtttag gatcgatcag attaagcaaa aaggcgatcg tcaaaccatg
    61021 agatacgact aaaacatttc caccaccatt tttttcgcag tgatgggcga tatcttcaaa
    61081 tcctgtccat acacggtctc gcaatgtttc ataagtttcc gcccacccag cggtatctaa
    61141 gttgtataca gcattagcaa tttgttcaaa agaaacttct gtggtaaaca tttcatcata
    61201 tgttttaaaa ttcaatacac gagggatcac accccacatt tccatgtcat atccaccttc
    61261 aagagacccg aaacaccatt ctctaatcct tttttcaata aaataaggaa tttcttttcc
    61321 cttagaatgt tctcctagaa taatacgggc agtttcgatt gcacgaccac tgtcactaga
    61381 ataagcaaat gaaaagtcga cattttttaa cccgcgacct aaataatgaa tgccttcttg
    61441 tccttgcttt gtgaggggtg tatcacacca gccttgtact cgttcaattg tattgaacat
    61501 cgtttttccg tgtcgtacga tataaagtgt agtttctgtc attttattct cctcctaagt
    61561 cgttttaacg attgaaaaaa ggatttgtcc ttttttcctg ttcaatcgtc gttgcatcgc
    61621 catgtcctgg atagactgca aattcatcag gtaatacaaa cagctgggtc cgaatactat
    61681 gaagtaattg ttctaaattc cctgtataca gatcggttct accgatactt cctctaaaca
    61741 gggcgtcgcc agaaacgaca aaatcatcaa aaataaagct gacgcttcct atagaatgcc
    61801 ctggggtagg gacgacgcga aaagacatgt ttcctaaagt ataagttttc atttcaaatt
    61861 catattctgc aggcgagaca ataacatcag caatatcatc atgtctaccg agtccagaaa
    61921 gattcaaaat cggatcacct agccattttt gctctagtgg gctgacataa actgggattt
    61981 gataatacgc gcgtaaaggt tccactgcac cgatatgatc gtagtgagta tgggtaagca
    62041 aaatcgctac cggctgttgg tttgtttttt tgatttcttg tttgattcgt tccgcatctt
    62101 ctccagggtc aatgatcaaa agtgcttctt cgttgtagac caaataacag ttttcttcaa
    62161 tcattcctgt ttttaatctt tcgatatgca tgtgcattcc tccttgatag ccaaaacttc
    62221 tttcatccat tagtataacg cattcattgc taggaaacaa aaacaggtac ttatttctct
    62281 ttacttatct gaattatcag actatatgaa aagtatttgg atattctaaa gagaaggaaa
    62341 gtaagttttc atagcatttt aatatgtcct tgttacatta gattcatcca aaaatccaaa
    62401 ttctatatcc tatatccctg cctaatcagc agggtgtttt tttatgtttg gaaagagagc
    62461 tctttttgct aaactaggtt ttttcaggta atcttagaga gaaagaggtg gtggaaatga
    62521 tcaaacagtc aaaaatatct gtgcggcact tagtagaatt tattttgcga cgtggaagta
    62581 tcgacaatcg taaaaaaagt aaccatactg cattagaagg agcgagaatc caccgcaagc
    62641 tccaaaaaga tgcgggagaa aactatgaaa aagaagtctt tttaaaaaca acagttcaat
    62701 tagatgaata ttacctgatc gttgaaggcc gtgcagacgg tattttcaaa agagacggta
    62761 tttattatat agatgaaatc aaaacctccg aaccacgatt tgaagattta gaaccagaac
    62821 aagtggaact tttctttcat caagcaaggg tgtatgccta tatctatagt caagaacatg
    62881 atttacaaga aataaatctg caattgactt acttccagac ggcagaagaa gtgatcacga
    62941 gaaaggttga acatcagaca agagagcagt tagaaacttt ttttaaaaag ctgactgaag
    63001 attataaaga atggcttgtt tttcaagaaa actggcgcac tgtacgcaat gcctctttga
    63061 tggctttgaa attcccccat gatgagtata ggaaaggtca gcgtgaacta gcggtagctg
    63121 cttataaaac gatccgtacg aaacagaaat tgtttgtaga agcacctacc ggaacaggaa
    63181 aaacaatctc tacgttattc cccgctttga aagctgtagg ggaagaagag ggagaaaaga
    63241 tcttttatct aacggctaaa acaataacta gacaagtagc tgaagatgct atgacagcat
    63301 taaaagatac aggagctgaa gtaaaaagtg tcactttgac ggcgaaagac aagatctgct
    63361 ttttagatga gacgacctgt aatccagatc agtgcccata tgccaatggt tattacaatc
    63421 ggattaatga aggtttatgg gatcttttga atcatgagaa tcagatcact cgtgaagtca
    63481 tcgaaactta tgcaagaaag catacacttt gtcctttcga actatcatta gatgtcagta
    63541 tctggtgtga cgtaatcatt ggggattaca attatttatt tgatccaact gtttatttaa
    63601 gacgtttttt tgaagatgaa aaaaacgagg actatctgtt tttgattgac gaagcacata
    63661 atttggtgaa tcgttcccga gagatgtatt cggcagaact atcttatgaa aaaacgaaac
    63721 gaagtaagga agctatacca aaggaattca aaaaacttca tcgccgtttc aacaaactat
    63781 taaaagaatt tgattcgatc cgagaaatag caaaagaaga tcattgggat tatcatcatc
    63841 aaaaagcacc ggctgaatct ttagtaaagg caggttatca attaagcgaa aaaattaaag
    63901 aatggctggc agaatttcct gagcacccat cacaagaaca attgcttcct tattattttg
    63961 atttgcttca ttttttgaag gtcagtgaat tttatgatga tcactacgaa acaacagtcg
    64021 aaaaaacgta tcgcgatctg atagtaaaag agttttgtat tgatccaagc ttgtttcttg
    64081 agcagtcgtt ggataaagga aaaagcagta ttcttttttc tgctagtttt tcaccactct
    64141 cttattatca ggaaacactg gggggacaaa aaagtctggc ttacaaactg ccgagtcctt
    64201 tcccagaagg aaaccagcaa gttttcatcg caaattacct agagacgact tatcgaaaaa
    64261 gggaacacag tctggcaaaa ttagtagaga cgattcatgc ttttgctgat ggaaaggtcg
    64321 ggaattattt cgttttcttt ccttcttatc aatatttaga tcttgcggtg gaagcattca
    64381 gacagcaata tcccgacagc aatgtgctaa tccaagaaac ggacatgaat gaggaagaac
    64441 gcgaacgatt tttaacaaaa tttcaaacaa atcccgctga aacattgatt ggattttgcg
    64501 tacttggcgg gattttttca gaaggaatcg atttgagagg ggaccgattg atcggcagta
    64561 tgatcgtagg tgtaggtttg ccgcagatga atcatgagca ggaactgatc aaatcatatt
    64621 atgatgagaa ggaacacctt ggatttgctt acgcttatca gcttcctgga atgaacaaag
    64681 tcttgcaagc tggtggtcga gtgattcgtg gcatggcgga tcaaggaatc gttgtattgg
    64741 cagatcaacg attttctagt ttttcttatc gacgtttatt cccacaacat tggcaaacag
    64801 caagagaagt acgaagtgtt ggggaattgg aagaaggaat acagcgtttt tggttatcaa
    64861 aagaagcagt aaatcaaaaa aacgaggaat gacagacgtc agcctcgttt tttgattaac
    64921 gatagttagt gaattgcaac tcgattggta agtcaagttt acgtaataaa gcaatcacag
    64981 cttgtagatc atcacggttc ttacctgtca ctctaatctg atcttcttgg atttgtgctt
    65041 ttactttcag tttttcattt ttgatagctg tagtgatttt cttcgcgttt tcacgatcga
    65101 ttccgttgac caaatcggca gtttgtcgag ctttgccacc tagagcgtgt tctgattcgg
    65161 aaaaatggat atttttgatt ggcacatttc ttttgatcag cttaccaaat aatacatctt
    65221 tgatctgttc gattttgaaa tcatcatcgc tcaatacaac tagattgctg ttctctaatt
    65281 tgatttctac tgtagaatcc ttgaagtcaa agcgattgga caattctttt gttgtttgct
    65341 gaatcgcatt ttttacttct tctgtattga cttctgatac aatgtcgaag ctagcatctt
    65401 ttgccatagg aaccctcctg atatacatat aaatatttac catacatcta tcttatcata
    65461 ggattagggc agattcttac ctgtttgcta aaattcttct catttgagtt cgaagtattt
    65521 tattttaata gataaactgt tattcttaca tttggaatac caaagtaggg gagaggtgct
    65581 tgtgagtaaa attatgactg cgtatcataa aaatgaaaga ttaaagaaaa tagtggttat
    65641 tttgataacc ggattgaccg ctgcggtcgc tttaaattat tttttgattc cagccaatgt
    65701 cttttctgca gggatgaatg ggatcgctca aatcatttcc tcgcttttat atgaatggtt
    65761 tcatatcaag atagacacag gaacatttat ttttcttttg aatatccctg tgtttgtatt
    65821 agggtttctc aaagtaggaa aacgagcaac gatccttagt tttgtgaatg ttgtatgtgt
    65881 atcggtgatg acgatgctgc ttccagtcgg acagatcact gataatattt tgatgaatgc
    65941 cctagtaggc ggtgttttat taggtcttgg cgtaggatta tctttaaaaa tgggctttac
    66001 aactggtggg atggatatca tctctcttgt actttcacaa acaactggaa aaacagtcgg
    66061 aaactacatg ctgattctaa atggtatcat tgtagtcatc gcaggcttct tctttaactg
    66121 ggaaagtgcc ttgtatacga ttatttccat ttatgcaatg actcaagtaa ttgatgccat
    66181 ccacacgagt catcaaaaag taacagcaat gatcgttacc gtcaagccag aagaggtaac
    66241 agaagaacta gcaaaacgga tggtacgtgg aatgacatta ctgccgtctg tcggtggcta
    66301 ttcaaaaaaa caagggaaca tgatcatgat ggtcatcacc cgctacgaaa tgtatgatct
    66361 tgatcagatc gttcatgaaa tcgacgacga tgcatttatc aatattttac caactcattc
    66421 agtatttggc cggtttgcaa atgaaaacga acaaaaaatg tatcgaacaa ctggcgtatt
    66481 gccagaaata aaaaatcgtt cacaaaaaaa atgaggaatc aggtttattt tagaataaat
    66541 ttaatctatt acatctaata agaacatttt tataaattta aacgagaatc ttacttctaa
    66601 attctcattt acaagcagct aaaaaaagag tatgataaat aaaaatcgtg ttaaggagtg
    66661 tcgtacgatg agcgaagcaa accaagaacg gtctggacaa gaaatagaac taatcaaaga
    66721 acgtattctt gctgccttgg aaatggtgat tgatcctgaa ttaggaatcg acatcgtcaa
    66781 tttaggcttg atttatgata tagaattcaa tcctgaaaat ggagaaacag tcattaagat
    66841 gactctaaca actatgggct gtccattagc agatatttta acagaatcta tccatgacgc
    66901 attaaaagaa gtgccagaag taacgaaagc agaagtcaaa ctagtctggt atccagcatg
    66961 gacgacagat aagatgagcc gttatgcacg gattgcgttg gggatacgat agaaatcttg
    67021 gctgactagc tatgagaagc aaataggaaa aacaaaaaag ccttttatcc tttctagaag
    67081 aaaagagata aaaggtttct ttgtttataa aattttgtct gctatattat aagttaatta
    67141 aataaatgtg ttctattgat aaaggacatt caaatccctt tccttttgga atccttcttt
    67201 tttacatcag tttgattttc gtatagtatt ctttctcatc ttcacgataa atcgcagccc
    67261 catttttttg ttgtgcttta agactgccaa gatttttttt atttacagac aaaagtgcct
    67321 catcaatgcc gatttttttt gcctcttgta gtactaatgc taatccttgt gtggcatatc
    67381 cttttttacg ataattaggg gagatgccgt aaccaatatg gccagcgccg tttcttaatg
    67441 tttcgttcag tttatggcgc aaattaaaaa ttcctacata ctcatgttca tcactttcta
    67501 aaataaagac ggttgcggga acgtaactct caggaagatc gattcctaaa gacgtttttt
    67561 cgcatttttt tacataatct aaaaattcag caaatgtata gccataagca ttgttgataa
    67621 atccactctc atttggtcca aagctagttt gtagctgata agctttcttt aaatctgctt
    67681 cccatagttt gatcagtttc atcgtattca cctctttaag taaccttatt catttgtttt
    67741 ctactatagc actattgttg taaatagcca tgcctatttt tatttattgt gtcatatcgt
    67801 attaagaatc atcgaacaat acaaatatga attacaataa aaaaattatt tgaagttcgt
    67861 tcattcgtta cttatgatct ctagaagaaa gaaaacgtga attacttcag cgtctagaat
    67921 tccagaagga tagtaatcaa cagaagaaat aatcaatatc caaatgaaag aagatagctg
    67981 aatcgagaaa aagaggaaaa aagaaatgaa gaaaaaaata aaatagttac taatcccaga
    68041 gtcgttcgtt taacattgaa atcgatcata tggataaaac agattttttc accaaccctc
    68101 aatagatgat ggagttgctc ggatgaatta tttgtgtaga tggcataagg gagggcgtac
    68161 agaaatgaaa aagaacaaaa atatgttctt gacaaaaaac acatgtaatc ctaaactaaa
    68221 agatgtgatg cggctatggc ggaattggca gacgcgctag cttcaggcgc tagtgagggt
    68281 gacctcgtgg aagttcgagt cttcttagcc gcataatttg tttttttata aagagaaggt
    68341 acacctggag cgatgagaaa gaaaataaca tattatctaa aagaatatag gaaaaatttt
    68401 aaacaacgga accagattca aataaatcaa aaaaaatttg tgtgaaggtg ggacagttta
    68461 aaacctaata gattgattca aatagtagat ttatagattc tgatacaaga aatactggca
    68521 aacgatagtt aaggaataaa gactaaactt aaactcaaaa agaggtcatg cgaaagaata
    68581 aatcgcatga cctcttacta tattttgaat gtgtttaaac gttcaaataa tgaatcttag
    68641 taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta
    68701 acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa
    68761 gaatctaatt ggtaacggcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat
    68821 tctttttgtg cgatccattc ttttgcagag cttgtacttg ctggagtagc agcagtttct
    68881 gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtgtttc cgtttgaacc
    68941 gtttcttgta caacaggttg ttcagcttga acaggttcag cttggactgg tgcttcttga
    69001 acggctgctt gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt
    69061 tgtcctgagt a
//
LOCUS       DAZKMZ010000006        64433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000006 DAZKMZ010000000
VERSION     DAZKMZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.006"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..2315
                     /gene="acm"
                     /locus_tag="ACG8M5_000478"
     CDS             150..2315
                     /gene="acm"
                     /locus_tag="ACG8M5_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0357760.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2587..3072)
                     /locus_tag="ACG8M5_000479"
     CDS             complement(2587..3072)
                     /locus_tag="ACG8M5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0357761.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3162..3507
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3552..4193
                     /locus_tag="ACG8M5_000480"
     CDS             3552..4193
                     /locus_tag="ACG8M5_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0357762.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4212..4847
                     /locus_tag="ACG8M5_000481"
     CDS             4212..4847
                     /locus_tag="ACG8M5_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357763.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4865..5680
                     /locus_tag="ACG8M5_000482"
     CDS             4865..5680
                     /locus_tag="ACG8M5_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0357764.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5999..7435
                     /locus_tag="ACG8M5_000483"
     CDS             5999..7435
                     /locus_tag="ACG8M5_000483"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0357765.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7624..10071
                     /locus_tag="ACG8M5_000484"
     CDS             7624..10071
                     /locus_tag="ACG8M5_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0357766.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10301..11368
                     /gene="rlmN"
                     /locus_tag="ACG8M5_000485"
     CDS             10301..11368
                     /gene="rlmN"
                     /locus_tag="ACG8M5_000485"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0357767.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11358..11564
                     /locus_tag="ACG8M5_000486"
     CDS             11358..11564
                     /locus_tag="ACG8M5_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357768.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11561..12664)
                     /locus_tag="ACG8M5_000487"
     CDS             complement(11561..12664)
                     /locus_tag="ACG8M5_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0357769.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13217..13765
                     /gene="aac(6')"
                     /locus_tag="ACG8M5_000488"
     CDS             13217..13765
                     /gene="aac(6')"
                     /locus_tag="ACG8M5_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0357770.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(14013..14132)
                     /locus_tag="ACG8M5_000489"
     CDS             complement(14013..14132)
                     /locus_tag="ACG8M5_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0357771.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            14311..15972
                     /locus_tag="ACG8M5_000490"
     CDS             14311..15972
                     /locus_tag="ACG8M5_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0357772.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16042..16389)
                     /locus_tag="ACG8M5_000491"
     CDS             complement(16042..16389)
                     /locus_tag="ACG8M5_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0357773.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16473..17414
                     /gene="opp3b"
                     /locus_tag="ACG8M5_000492"
     CDS             16473..17414
                     /gene="opp3b"
                     /locus_tag="ACG8M5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0357774.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17415..18458
                     /locus_tag="ACG8M5_000493"
     CDS             17415..18458
                     /locus_tag="ACG8M5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357775.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18464..19534
                     /locus_tag="ACG8M5_000494"
     CDS             18464..19534
                     /locus_tag="ACG8M5_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357776.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19524..20477
                     /locus_tag="ACG8M5_000495"
     CDS             19524..20477
                     /locus_tag="ACG8M5_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357777.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20489..21499
                     /locus_tag="ACG8M5_000496"
     CDS             20489..21499
                     /locus_tag="ACG8M5_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0357778.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21784..22605)
                     /locus_tag="ACG8M5_000497"
     CDS             complement(21784..22605)
                     /locus_tag="ACG8M5_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0357779.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22702..22980)
                     /locus_tag="ACG8M5_000498"
     CDS             complement(22702..22980)
                     /locus_tag="ACG8M5_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357780.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23248..25257
                     /gene="metG"
                     /locus_tag="ACG8M5_000499"
     CDS             23248..25257
                     /gene="metG"
                     /locus_tag="ACG8M5_000499"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0357781.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25736..26323)
                     /locus_tag="ACG8M5_000500"
     CDS             complement(25736..26323)
                     /locus_tag="ACG8M5_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357782.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26498..27820
                     /locus_tag="ACG8M5_000501"
     CDS             26498..27820
                     /locus_tag="ACG8M5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0357783.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27975..28751
                     /locus_tag="ACG8M5_000502"
     CDS             27975..28751
                     /locus_tag="ACG8M5_000502"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0357784.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28744..29313
                     /gene="rnmV"
                     /locus_tag="ACG8M5_000503"
     CDS             28744..29313
                     /gene="rnmV"
                     /locus_tag="ACG8M5_000503"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0357785.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29306..30190
                     /gene="rsmA"
                     /locus_tag="ACG8M5_000504"
     CDS             29306..30190
                     /gene="rsmA"
                     /locus_tag="ACG8M5_000504"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0357786.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30641..31270
                     /locus_tag="ACG8M5_000505"
     CDS             30641..31270
                     /locus_tag="ACG8M5_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357787.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31394..32503)
                     /locus_tag="ACG8M5_000506"
     CDS             complement(31394..32503)
                     /locus_tag="ACG8M5_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357788.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32712..33134
                     /gene="mgsA"
                     /locus_tag="ACG8M5_000507"
     CDS             32712..33134
                     /gene="mgsA"
                     /locus_tag="ACG8M5_000507"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0357789.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      33321..33419
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            33457..33993
                     /locus_tag="ACG8M5_000508"
     CDS             33457..33993
                     /locus_tag="ACG8M5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0357790.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34033..35796)
                     /locus_tag="ACG8M5_000509"
     CDS             complement(34033..35796)
                     /locus_tag="ACG8M5_000509"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357791.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35799..37514)
                     /locus_tag="ACG8M5_000510"
     CDS             complement(35799..37514)
                     /locus_tag="ACG8M5_000510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357792.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38293..38517)
                     /locus_tag="ACG8M5_000511"
     CDS             complement(38293..38517)
                     /locus_tag="ACG8M5_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357793.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38708..41485
                     /locus_tag="ACG8M5_000512"
     CDS             38708..41485
                     /locus_tag="ACG8M5_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0357794.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41566..41985
                     /locus_tag="ACG8M5_000513"
     CDS             41566..41985
                     /locus_tag="ACG8M5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0357795.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            41997..42467
                     /locus_tag="ACG8M5_000514"
     CDS             41997..42467
                     /locus_tag="ACG8M5_000514"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0357796.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42484..43263
                     /locus_tag="ACG8M5_000515"
     CDS             42484..43263
                     /locus_tag="ACG8M5_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0357797.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43250..44071
                     /locus_tag="ACG8M5_000516"
     CDS             43250..44071
                     /locus_tag="ACG8M5_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0357798.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44088..45137
                     /locus_tag="ACG8M5_000517"
     CDS             44088..45137
                     /locus_tag="ACG8M5_000517"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0357799.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45150..46154
                     /locus_tag="ACG8M5_000518"
     CDS             45150..46154
                     /locus_tag="ACG8M5_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0357800.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            46787..47806
                     /locus_tag="ACG8M5_000519"
     CDS             46787..47806
                     /locus_tag="ACG8M5_000519"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0357801.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            47849..48730
                     /gene="galU"
                     /locus_tag="ACG8M5_000520"
     CDS             47849..48730
                     /gene="galU"
                     /locus_tag="ACG8M5_000520"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0357802.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            49030..49407
                     /locus_tag="ACG8M5_000521"
     CDS             49030..49407
                     /locus_tag="ACG8M5_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357803.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            50013..50627
                     /locus_tag="ACG8M5_000522"
     CDS             50013..50627
                     /locus_tag="ACG8M5_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0357804.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            51400..53139
                     /gene="yfcC"
                     /locus_tag="ACG8M5_000523"
     CDS             51400..53139
                     /gene="yfcC"
                     /locus_tag="ACG8M5_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0357805.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            53296..54480
                     /gene="iadA"
                     /locus_tag="ACG8M5_000524"
     CDS             53296..54480
                     /gene="iadA"
                     /locus_tag="ACG8M5_000524"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0357806.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            54591..54794
                     /locus_tag="ACG8M5_000525"
     CDS             54591..54794
                     /locus_tag="ACG8M5_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0357807.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(54895..55557)
                     /locus_tag="ACG8M5_000526"
     CDS             complement(54895..55557)
                     /locus_tag="ACG8M5_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357808.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(55570..57072)
                     /locus_tag="ACG8M5_000527"
     CDS             complement(55570..57072)
                     /locus_tag="ACG8M5_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357809.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            57620..59299
                     /locus_tag="ACG8M5_000528"
     CDS             57620..59299
                     /locus_tag="ACG8M5_000528"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0357810.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            59426..59905
                     /locus_tag="ACG8M5_000529"
     CDS             59426..59905
                     /locus_tag="ACG8M5_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0357811.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            60124..60804
                     /locus_tag="ACG8M5_000530"
     CDS             60124..60804
                     /locus_tag="ACG8M5_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0357812.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            60812..62143
                     /locus_tag="ACG8M5_000531"
     CDS             60812..62143
                     /locus_tag="ACG8M5_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0357813.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            62158..63120
                     /locus_tag="ACG8M5_000532"
     CDS             62158..63120
                     /locus_tag="ACG8M5_000532"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0357814.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            63231..63569
                     /locus_tag="ACG8M5_000533"
     CDS             63231..63569
                     /locus_tag="ACG8M5_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0357815.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            63648..64355
                     /locus_tag="ACG8M5_000534"
     CDS             63648..64355
                     /locus_tag="ACG8M5_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0357816.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT        21221 a        11142 c        13450 g        18620 t
ORIGIN      
        1 gcatatttat tttttataaa tatttgtaaa ataactatca attttttagt taaaagattg
       61 gaaatgagtg ggggaggaat tgaagaaagg atttttgaga gatgatatag taggttttat
      121 ttttagttaa aataggggag atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa
      181 tgttatttat tattactaat ataacatcac tgattcctgt tcatgtctat gcggatgcag
      241 gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat attacagatg
      301 gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg
      361 attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag
      421 aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga aaagattctg
      481 cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt tgggggcaat
      541 ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga ccattcaccg
      601 tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct ggttcttctt
      661 ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag cacattcgat
      721 ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa atcagtgatg
      781 aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa atacacttag
      841 gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa gatttcccca
      901 gcgctacatt taatttttca gtaactgata attacattga aataactata ccaaaaaact
      961 ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac ccagaacaaa
     1021 taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct gctgttgatg
     1081 gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc aacggtacag
     1141 ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt atcccaaatg
     1201 tgactttcga attaagacga gcagatgaac agcctattca aggacagtct agtatcctgt
     1261 tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga gactatgttg
     1321 taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca aatgaactca
     1381 aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat aacaagaaaa
     1441 aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca agaccgagta
     1501 tttattttaa attattcaga gcaagtacaa acaattggga accagttcct gatgcagaaa
     1561 caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa cagtatgatg
     1621 atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga aatgattatg
     1681 tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag aataaagaag
     1741 tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac ggcaaacgac
     1801 cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac aaagaagtaa
     1861 gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga gacgggcaag
     1921 aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg attgacgggt
     1981 ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca ggtagccata
     2041 taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga aatctattct
     2101 ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga agcaattata
     2161 ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt cttcctaaaa
     2221 caagtagtaa aaaatctagt tttgctgtaa tcattggttt attgatagta actatctgtg
     2281 ctgggatatt cttacaaaaa tataaaaaag tttagttaga atataatatt ttttgttaaa
     2341 tatgactgaa atcaaaagaa taaaattccg atttataatc gaaaaaagaa gcatttttat
     2401 atatgcttct tttttcgtag aatcaataaa tatggatttg gctaactatg gaagaatact
     2461 cgtattagtt ttcagcgttc atactagttg ttacattgtt tgattggcca aaagaatttc
     2521 tgatatattt tgtgctaaat aaagaaaaag acgcttgaat tgggaaataa gaaaactact
     2581 ctatcattac aaattttcat aaactggcag atgttctaag taaacaagta tcttttcaaa
     2641 gtcggaaaaa tccataatca acgtagtggt attgatattt ggatgaaagc ctacggtatc
     2701 ttgacgatca atagcagcat cgatcacgac ttggattttt ttctctttgt cgtgcatcaa
     2761 tgctaatgga gtcactgtac ctgctggaac gcctaggagg tcaggaagct gttcttcaga
     2821 aacaaaagat aagcgctttt cttttaattg ttcagctagt tgttttaaat cggcgtgctt
     2881 ttcatcaggc aagatgacaa gatagatttg ttttcctttt cttgatttca ataatagatt
     2941 ttttacttgc ggaccaggta gggaaaaagg gacatttttg actgacgtaa tcgcaggatg
     3001 atcgactttt tgaaaagaga tacccagttt ttttagtagt tcataagctt cttgttcggt
     3061 tgcacgactc atcgcgcatc accctccttt ttttattagt gtatcataaa aaagagttga
     3121 caaatgaaaa ataatcatgt aaattacacg taaagttaaa acaaatgaca tcgaaaagaa
     3181 gagtagccgt taggtgtttt tagagagtct ctggttggtg gaaagaggca gcagttaacc
     3241 gtgaaacaca tcttggagtc gattttctga agcgtatagt aggaaaatcc ggggagcccg
     3301 ttacagctgc ggtcgttgct agatgaccag ataaggctgg cttttccgtg aggagtcggt
     3361 aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt cggatcaaag gactttttgt
     3421 tttcatctga acggcaacga ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg
     3481 tggaaccacg tataatacgt cctcttggca tttgccaaga ggattttttt attttaaagg
     3541 aggaaaagaa gatggagtat ttgattgaag ttatgccgca attattagta ggagcactca
     3601 caacattgaa attattttta tttaccttga ttggctcatt accattaggg attgttttag
     3661 cattaggatt gtctttgcat tttaagccgc tacgctactt gcttcaaaca tatgtatggg
     3721 ttatgcgggg aacaccgctg ttgttgcaac tgatttttat tttttatggg ttgccgacga
     3781 ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc ctttatctta aactatgcag
     3841 cttattttgc agagatcttt cgtggaggga tacaatcgat tcctcaaggt caatatgaag
     3901 cagcgcaagt tttgcaatta agtccctttc aaacggtgac gaaaatcgtc atcccacaag
     3961 tgacaaagat tgttttacct tctgtaggga atgaagttat caacttggtc aaagattctt
     4021 ccttgatcta tatattaggt ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac
     4081 gcgacgcgac tttactgcct ttgctaggcg tggcaatcat ctatcttaca ttgactggta
     4141 ttctaacaat cataatgaaa caagttgaag gaaaattaaa ttattaccgc taactgatag
     4201 gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa
     4261 tcaaagattt gaatctaatc attccagatg ggaaaacatt agctatcgta ggtccttccg
     4321 gtggcggtaa aaccacgctt ttacgtatct tggctggctt agaaagttct gacagcggag
     4381 aattttattt agacggtgta ccgttcaacc cattcgagct taaaatgaaa gaacaagttg
     4441 taggagtcgt attccaagac ttccagctat ttccacatct gaatattttt gaaaacatta
     4501 ccttagcacc aaaaatggta ttgaaacaga caaaagaaga atacaccaaa aaagcagaag
     4561 aattagcggc ttcattaggg attagtgatt tattgaatat ctatccttat cagctttctg
     4621 gcggacaaaa acagcgggta gctattgcca gagcattggc tatgaatcca cgggtattgg
     4681 cttatgatga accaacaagt gctttagatc ctgaattacg ccagcaagtc gagcgtttga
     4741 tcctatcatt gaaagaagat ggggtaaccc aaattattgt cacccatgat ttagtatttg
     4801 cagaaaatgt cggtgatcaa ttactaaaag tttccccaat tcattaagaa aggagaagat
     4861 agagatgaaa aaaaccagtt ttctattcgc tttgatcgca ggtcttcttc tttttgcagg
     4921 ctgttcgaac aaaaaaactt cagcagataa ttgggcgact atcaacgaag agaaaaaaat
     4981 cgttataggt ttagacgata catttgttcc tatgggattt agagataaag acgggaagct
     5041 cacaggattt gatattgatt tagccaaagc agtctttgaa caatatggga ttacggtaga
     5101 ctttcagccc attgactggt caatgaagga atttgaactg aacaatggaa cgatcgattt
     5161 gatatggaac ggctattcca aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc
     5221 ttatatggaa aacgatcaag tgctgatcac acctaaagaa tcaggaatca caaactttgg
     5281 acaaatgaat aataagagat taggtgcgca aaatgggtca tctggttatg atgtatttac
     5341 gaaacaacca gaagtattaa aagagatagt caaaaaccaa gatgctgttt tgtatgatag
     5401 ttttaatgaa gcattgatcg atctgaaaag cagacgggtc gatggtctgc tgatggataa
     5461 agtatacgca gactattatt tgaaacaaag aaacgaacta gaagacttca acataacaaa
     5521 aggttcttat gagagtgagg attttgcagt tggtgcacgc aagtcagata gtacattagt
     5581 gaaaaaaatc aatgaagccc tagatcaact agagaaaaca ggtgaattcc aaaaaatatc
     5641 cgataaatgg tttggcgaag atgttacgcc gagaaattaa caaatagggg actgtgacaa
     5701 aagacgtgtc acagtccctt tttccgaata aacagtgttc tcctgccaac tccttgtgac
     5761 tcggagctga atggcaggct cacaacctct tggtaaacgg tgttcaaaag ccagtttttc
     5821 ggtattaagc caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttact
     5881 actcgaaact aagcgacttt ttcacaacct cctttacata attttatttt gttgtggaag
     5941 gggtttcgtg ttattctaaa agaggaacga ttgaaggtta taggaaagga agagatgaat
     6001 ggaacacaaa gaacttacct catttccaaa agattttctg tggggctctg cttctgcagc
     6061 gtatcaagta gaaggagctt ggcaagaaga tgggaaagga gaatcagtct gggatcgatt
     6121 tgtacggata cctggaaaga catttaaagg aacgaacggt gatttggcag tagaccacta
     6181 tcatcgttat aaagaagata ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt
     6241 ctctgtagct tggacacgga tttttcctaa tggacgagga gaagtcaatc aagctgggct
     6301 cgctttttat gagagattga tcgacgaatt gattgaaaac gatattgaac ctatgttgac
     6361 tttatatcat tgggatcttc cacaagcatt gcaagatgaa tataatggtt gggaatcacg
     6421 tcagatcatt gaagatttca ctcattatgc agaaacatta tttgaggcat tccgcgggaa
     6481 agtccattac tggattagtt tgaatgaaca aaatatcttc acttcattag gttatttgtt
     6541 agcagcacat ccgccaggtg tgacagaccc gaaacgaatg tatgaagtta accatattgc
     6601 taatttagca aatgcatctg tcatcaataa atttcacgag ttagaaatgc ctggaaaaat
     6661 cggaccaagc tttgcgtata cgccaaatta tccaattgac agtaatcctg aaaatgttct
     6721 ggctgctgaa aatgcagaag acctgatggc caattattgg atggatgtct atatgtgggg
     6781 aaaatatccg attgctgcca tgaggttctt agaagaaaaa ggatgggcac caacgattga
     6841 agctggcgat gcagaattgc tagaatctgc taaaccagat tttcttggga tcaattacta
     6901 tcaaacggcg acgaatgcat tcaatccatt aaacggagtt ggtgctggaa aaatgaatac
     6961 gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga
     7021 gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat
     7081 cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt
     7141 aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta
     7201 cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg
     7261 ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata
     7321 tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa
     7381 agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac
     7441 atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt
     7501 agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa
     7561 acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa
     7621 taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag
     7681 caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt
     7741 gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg
     7801 atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg
     7861 attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata
     7921 ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa actccatact
     7981 ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt
     8041 caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac
     8101 ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca
     8161 ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa
     8221 cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag
     8281 gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa
     8341 gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa
     8401 aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt
     8461 ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat
     8521 acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt
     8581 acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca
     8641 gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa
     8701 aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc
     8761 aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa
     8821 gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc
     8881 cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag
     8941 atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta
     9001 ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag
     9061 atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg
     9121 attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat
     9181 ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg
     9241 acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga
     9301 tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa
     9361 cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta
     9421 gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt
     9481 ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt
     9541 gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta
     9601 tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg
     9661 cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca
     9721 ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct
     9781 cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag
     9841 ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt
     9901 atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat
     9961 gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc
    10021 attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt
    10081 gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa
    10141 aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt
    10201 gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta
    10261 tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta
    10321 tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg
    10381 agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac
    10441 gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa
    10501 acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga
    10561 taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac
    10621 gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa
    10681 ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac attattttga
    10741 tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt
    10801 tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc
    10861 aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt
    10921 cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt
    10981 gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt
    11041 ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca
    11101 agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa
    11161 attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg
    11221 cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg
    11281 tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa
    11341 gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa
    11401 aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag
    11461 cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca
    11521 atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc
    11581 cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt
    11641 tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta
    11701 agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt
    11761 ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca
    11821 ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta
    11881 gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta
    11941 aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg
    12001 agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa
    12061 tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag
    12121 cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt
    12181 tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag
    12241 gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg
    12301 gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa
    12361 tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt
    12421 ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc
    12481 agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct
    12541 ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc
    12601 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
    12661 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
    12721 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
    12781 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt
    12841 gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt
    12901 ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt
    12961 caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga
    13021 gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt
    13081 ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag
    13141 ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga
    13201 acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga
    13261 tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga
    13321 agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga
    13381 gttagtagga tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc
    13441 attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt
    13501 agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga
    13561 ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc
    13621 catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt
    13681 aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat
    13741 tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa
    13801 gaaataagaa ggaatttacg aaaattactt cttgaatttt tgtgaaattc caacttattt
    13861 ccgaagaagc tacttttgga acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc
    13921 gagtagtaag aaaaattttg tgaaaatggc tttctacatt tttgcataaa tttggcttaa
    13981 taccgagaaa ctggcttttg aacaccgttt attcagataa gggactgtga caagactttt
    14041 gtcacagttt ctttttgttt attctttttt atctttgtat ggcaataata gacaatataa
    14101 cgaaaattat ttaatggctt ttggaagacc atggaaaacc ttgtacaaag ctattttttc
    14161 tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag
    14221 tgttgatttt ttaataatta ttcattaaac tcttaaacaa cttcagaaaa tattaattat
    14281 tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt
    14341 gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt
    14401 tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa
    14461 atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac
    14521 gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc
    14581 gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca
    14641 aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca
    14701 gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa
    14761 gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat
    14821 gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca
    14881 tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca
    14941 agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca
    15001 gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt
    15061 tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat
    15121 ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca
    15181 gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag
    15241 aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa
    15301 gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca
    15361 gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt
    15421 tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt
    15481 gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa
    15541 tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt
    15601 ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga
    15661 tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca
    15721 tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca
    15781 acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc
    15841 tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa
    15901 aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac
    15961 gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg
    16021 caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag
    16081 agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga
    16141 gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg
    16201 ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa
    16261 cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat
    16321 gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt
    16381 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
    16441 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
    16501 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
    16561 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
    16621 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
    16681 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
    16741 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
    16801 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
    16861 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
    16921 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
    16981 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
    17041 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
    17101 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
    17161 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
    17221 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
    17281 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
    17341 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
    17401 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
    17461 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
    17521 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
    17581 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
    17641 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
    17701 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
    17761 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
    17821 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
    17881 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
    17941 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
    18001 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
    18061 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
    18121 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
    18181 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
    18241 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
    18301 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
    18361 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
    18421 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
    18481 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
    18541 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
    18601 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
    18661 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
    18721 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
    18781 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
    18841 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
    18901 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
    18961 gattgcactg gtatgttatc cggaaatact gattgctgat gaacctacaa cagcgcttga
    19021 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
    19081 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
    19141 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
    19201 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
    19261 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
    19321 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
    19381 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
    19441 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
    19501 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
    19561 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
    19621 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
    19681 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
    19741 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
    19801 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
    19861 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
    19921 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
    19981 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
    20041 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
    20101 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
    20161 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
    20221 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
    20281 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
    20341 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
    20401 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
    20461 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
    20521 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
    20581 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
    20641 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
    20701 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
    20761 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
    20821 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
    20881 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
    20941 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
    21001 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
    21061 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
    21121 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
    21181 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
    21241 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
    21301 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
    21361 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
    21421 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
    21481 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
    21541 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
    21601 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
    21661 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
    21721 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
    21781 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
    21841 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
    21901 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
    21961 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
    22021 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
    22081 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
    22141 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
    22201 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
    22261 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
    22321 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
    22381 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
    22441 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
    22501 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
    22561 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
    22621 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
    22681 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
    22741 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
    22801 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
    22861 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
    22921 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
    22981 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
    23041 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
    23101 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
    23161 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
    23221 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
    23281 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
    23341 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
    23401 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
    23461 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
    23521 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
    23581 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
    23641 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
    23701 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
    23761 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
    23821 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
    23881 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
    23941 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
    24001 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
    24061 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
    24121 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
    24181 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
    24241 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    24301 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    24361 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    24421 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    24481 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    24541 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    24601 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    24661 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    24721 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    24781 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    24841 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    24901 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    24961 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    25021 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    25081 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    25141 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    25201 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    25261 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    25321 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    25381 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    25441 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    25501 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    25561 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    25621 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    25681 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    25741 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    25801 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    25861 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    25921 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    25981 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    26041 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    26101 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    26161 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    26221 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    26281 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    26341 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    26401 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    26461 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    26521 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    26581 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    26641 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    26701 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    26761 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    26821 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    26881 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    26941 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    27001 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    27061 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    27121 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    27181 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    27241 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    27301 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    27361 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    27421 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    27481 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    27541 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    27601 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    27661 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    27721 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    27781 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    27841 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    27901 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    27961 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    28021 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    28081 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    28141 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    28201 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    28261 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    28321 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    28381 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    28441 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    28501 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    28561 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    28621 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    28681 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    28741 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    28801 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    28861 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    28921 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    28981 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    29041 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    29101 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    29161 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    29221 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    29281 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    29341 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    29401 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    29461 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    29521 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    29581 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    29641 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    29701 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    29761 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    29821 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    29881 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    29941 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    30001 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    30061 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    30121 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    30181 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    30241 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    30301 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    30361 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    30421 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    30481 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    30541 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    30601 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    30661 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    30721 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    30781 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    30841 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    30901 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    30961 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    31021 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    31081 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    31141 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    31201 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    31261 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    31321 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    31381 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    31441 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    31501 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    31561 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    31621 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    31681 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    31741 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    31801 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    31861 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    31921 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    31981 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    32041 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    32101 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    32161 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    32221 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    32281 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    32341 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    32401 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    32461 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
    32521 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
    32581 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
    32641 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
    32701 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
    32761 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
    32821 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
    32881 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
    32941 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
    33001 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
    33061 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
    33121 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
    33181 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
    33241 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
    33301 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
    33361 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
    33421 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
    33481 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
    33541 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
    33601 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
    33661 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
    33721 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
    33781 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
    33841 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
    33901 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
    33961 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
    34021 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
    34081 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
    34141 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
    34201 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
    34261 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
    34321 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
    34381 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
    34441 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
    34501 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
    34561 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
    34621 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
    34681 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
    34741 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
    34801 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
    34861 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
    34921 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
    34981 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
    35041 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
    35101 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
    35161 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
    35221 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
    35281 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
    35341 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
    35401 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
    35461 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
    35521 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
    35581 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
    35641 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    35701 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    35761 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    35821 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    35881 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    35941 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    36001 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    36061 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    36121 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    36181 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    36241 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    36301 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    36361 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    36421 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    36481 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    36541 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    36601 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    36661 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    36721 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    36781 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    36841 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    36901 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    36961 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    37021 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    37081 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    37141 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    37201 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    37261 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    37321 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    37381 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    37441 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    37501 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    37561 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    37621 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    37681 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    37741 aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact
    37801 tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt
    37861 gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt
    37921 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
    37981 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
    38041 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
    38101 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
    38161 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
    38221 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
    38281 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
    38341 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
    38401 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
    38461 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
    38521 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
    38581 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
    38641 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
    38701 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
    38761 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
    38821 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
    38881 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
    38941 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
    39001 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
    39061 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
    39121 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
    39181 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
    39241 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
    39301 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
    39361 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
    39421 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
    39481 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
    39541 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
    39601 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
    39661 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
    39721 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
    39781 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
    39841 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
    39901 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
    39961 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
    40021 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
    40081 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
    40141 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
    40201 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
    40261 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
    40321 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
    40381 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
    40441 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
    40501 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
    40561 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
    40621 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
    40681 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
    40741 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
    40801 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
    40861 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
    40921 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
    40981 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
    41041 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
    41101 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
    41161 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
    41221 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
    41281 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
    41341 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
    41401 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
    41461 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
    41521 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
    41581 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
    41641 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
    41701 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
    41761 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
    41821 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
    41881 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
    41941 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
    42001 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
    42061 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
    42121 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
    42181 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
    42241 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
    42301 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
    42361 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
    42421 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
    42481 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
    42541 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
    42601 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
    42661 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
    42721 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
    42781 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
    42841 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
    42901 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
    42961 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
    43021 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
    43081 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
    43141 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
    43201 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
    43261 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
    43321 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
    43381 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
    43441 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
    43501 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
    43561 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
    43621 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
    43681 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
    43741 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
    43801 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
    43861 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
    43921 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
    43981 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
    44041 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
    44101 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
    44161 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
    44221 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
    44281 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
    44341 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
    44401 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
    44461 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
    44521 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
    44581 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
    44641 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
    44701 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
    44761 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
    44821 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
    44881 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
    44941 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
    45001 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
    45061 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
    45121 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
    45181 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
    45241 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
    45301 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
    45361 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
    45421 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
    45481 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
    45541 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
    45601 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
    45661 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
    45721 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
    45781 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
    45841 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
    45901 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
    45961 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
    46021 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
    46081 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
    46141 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
    46201 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
    46261 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
    46321 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
    46381 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
    46441 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
    46501 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
    46561 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
    46621 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
    46681 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
    46741 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
    46801 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
    46861 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
    46921 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
    46981 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
    47041 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
    47101 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
    47161 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
    47221 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
    47281 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
    47341 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
    47401 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
    47461 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
    47521 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
    47581 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
    47641 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
    47701 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
    47761 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
    47821 gttaatcgtc gtttaggagg aacagcaagt gaaggtcaaa aaagcagtca ttcctgccgc
    47881 tgggctagga acacgttttt tgccggcaac aaaagccatg gcaaaagaaa tgcttccaat
    47941 cgtggataaa ccaacgatcc agtttattgt ggaagaagca ttggattcgg gaatcgaaga
    48001 catcttgatc gtaacgggaa aagaaaaaag acccattgaa gatcactttg atgcaaatat
    48061 cgaattggaa aacaatttgc gggaaaagga aaaaacagaa ttactcgctt tagttgaaga
    48121 aacgactcaa gtcaatcttc acttcatccg tcaatctcat ccaagaggac tgggggatgc
    48181 cgtgcttcaa gcaaaagcat ttataggaaa tgaaccgttt gtcgtcatgc tgggggatga
    48241 tttgatgaaa gatgatatcc cattgactca acaattgatg atggattatg aagaaacaca
    48301 agcttccgct gttgccgtca tgcgtgtacc agaaaatgaa acatctaaat acggaattat
    48361 tgatcctaaa acagatagcg aaaagggtcg ttgcagagta aaagggtttg tagaaaaacc
    48421 tgaaatggga aaagcaccaa gtaatttagc cattatcgga cgttatctac tgactcctaa
    48481 aatatttgag atattagaaa ctcaagaacc aggagcagga aacgagatac agctaacgga
    48541 tgcacttcag actcttaacc agacagaggc ggtctatgcg agagagttta aaggaaaaag
    48601 atacgacgta ggcgataagt taggatatat gaagacaaat attgaatatg gacttcaaca
    48661 ttcggaaatt gggacttcat taagtgcata tattatccag ttgagtaaaa agctaagaac
    48721 agaaagttaa ctggaaataa gtggcctgct gagaatgaaa ggctatttgt tattaaagcg
    48781 gcagcttgtg cgtcatttgc gcaactgtcg ttttagtttt ttgactctag atcagttgag
    48841 tcgtggaaaa cgaaagatag aaaaagactt tcgtgaatag attcagaaaa tattatgcta
    48901 atggtttaat ttttttttaa gttgtataaa agaaaatgat tacataagag aaatgaaagc
    48961 caagccagaa gattcatagt agaataaaaa ggcgaatttt ttaattataa aaaaaggtgt
    49021 gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat actcaagtgg
    49081 ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag tctatttatc
    49141 atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta tgcagcatcc
    49201 gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc ttgcgtattt
    49261 gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa aagaaagtct
    49321 cggataggca gaaaaggtag acatacaact actttttcta gtacagattc tctcgaacaa
    49381 agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa aagataaaat
    49441 aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc ttttttcact
    49501 tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc attccttttt
    49561 ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc ttgcttgcac
    49621 ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa aaaaggctcc
    49681 ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata caccctgacc
    49741 gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct aatctagggt
    49801 gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta tttgctatgt
    49861 caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca aagaccacat
    49921 gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag ctgtttctat
    49981 tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt gactattatc
    50041 accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct ggcacttgga
    50101 tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc acactaagtg
    50161 gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata ttgattgaaa
    50221 atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt attgatcagc
    50281 tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga aacccggcaa
    50341 tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt gttactggat
    50401 ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta tttgaatttg
    50461 ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact ttgccaaatc
    50521 tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg cagcaagttt
    50581 tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta tccacatgtt
    50641 gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga aaaaaagaaa
    50701 acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt ttaagatgga
    50761 tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag ttgtttcaca
    50821 gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt agtggaaaaa
    50881 gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt gatgaaagag
    50941 tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta ttgcaatgaa
    51001 gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga aataaaatac
    51061 acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt tttgcaatga
    51121 aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc tctaatctaa
    51181 aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat aaatggaaag
    51241 agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg attttctaat
    51301 aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat ccatttttta
    51361 ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac aaattcttca
    51421 ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt cattattttt
    51481 ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa gttcagcacg
    51541 caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt tttacgtcaa
    51601 gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca attagaagaa
    51661 ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg attggaaaaa
    51721 gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat ctccttgaca
    51781 gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa aaaattgcat
    51841 aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt aaattttgtt
    51901 tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc tgcattgatc
    51961 cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga cgcaggtatc
    52021 tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct attattcttt
    52081 gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc attctcaatg
    52141 gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt aaccgtgact
    52201 tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag cgtagcggtg
    52261 gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt gattatgtgg
    52321 gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa aatccgtaaa
    52381 aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca tatcaaaaag
    52441 acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt agagatgtta
    52501 gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat tccagaaatc
    52561 gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct tttcaaatta
    52621 gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc tgatttagct
    52681 ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg aggatctgat
    52741 gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc gttgaccggt
    52801 gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt caacttggtt
    52861 gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc actggcagat
    52921 ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc aggttttatg
    52981 gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc acggatcgaa
    53041 tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt tttaggaact
    53101 attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc tgtaaataaa
    53161 aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt aaaatcaaag
    53221 gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt atacttaaag
    53281 aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc agattactta
    53341 ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga acagctagaa
    53401 ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt actaaccccg
    53461 ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg ttatcaaaat
    53521 cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac agttgtcggt
    53581 tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa agcgaaaggt
    53641 ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt gccggtaaaa
    53701 acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat cggaatcgga
    53761 gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt cgctcgtgca
    53821 gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat caatgtccac
    53881 ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga aacagaaatt
    53941 ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt tgaagcttgc
    54001 gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga tcctgatttt
    54061 tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt gatcgaggaa
    54121 gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt gccttatttt
    54181 gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt gctagtaggg
    54241 atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg agctataacc
    54301 agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat tggtgctgat
    54361 gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat cgctaaagga
    54421 gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa aagtttttaa
    54481 tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt atctgttgtt
    54541 gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca atggcagatt
    54601 taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa gaagcatacg
    54661 gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca ctaggtgctg
    54721 acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt tctcgaaaag
    54781 acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa aatccgaacc
    54841 atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg ttttttaaac
    54901 ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc tttttgctac
    54961 ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga tttcttggaa
    55021 aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct catccgctac
    55081 gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct gctgacctcc
    55141 cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac gagccagtcg
    55201 atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca ttgctgctat
    55261 gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa taatcgagac
    55321 atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt gatagtatac
    55381 ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg tttttccaga
    55441 accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt ctacatcttt
    55501 ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc gtaacatcaa
    55561 attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc ctatagaagc
    55621 taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc ccaattgtgc
    55681 aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg gattattcgt
    55741 ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg gcatttgtcc
    55801 accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa gtagctgctg
    55861 tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg caagaatcat
    55921 acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt ctcctaaaga
    55981 tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga tcagagtaag
    56041 tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac ttgccacgtt
    56101 gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc taaatgtatc
    56161 tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg gatcagatag
    56221 agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt catttgcaaa
    56281 agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca tacccatact
    56341 gccgcttgtt tctctggatt cataaattcc tttgatcgtc atttcataag tggtatcttc
    56401 atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg tttctgctag
    56461 agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc cttcaccatc
    56521 cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg acacaccact
    56581 gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag caggttcacc
    56641 gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga tggcagttat
    56701 tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta cattatctaa
    56761 ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac tgcctggatc
    56821 tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt taaatgaagc
    56881 ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt gattcgctgc
    56941 ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa tggctgtgaa
    57001 cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc atgcacgttt
    57061 cataaaattc atattttgca tccctcctag tagatgcatt tgataataga caacctatct
    57121 taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc tgtatatata
    57181 aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg tttcaaaccg
    57241 acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag agggattttt
    57301 acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt gttgtataat
    57361 aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg caaaagatcc
    57421 attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga acgcggatgt
    57481 gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg catatgcttc
    57541 attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa cttggatgtc
    57601 ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg gcgttcgtga
    57661 aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt tagattgtgg
    57721 gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg attttacgta
    57781 tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac atgcagatgc
    57841 gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg ggacagagtt
    57901 gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag gatttaagga
    57961 tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag tgagcttttt
    58021 ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa aagaaggtag
    58081 tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt atcaaacaga
    58141 ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa gtgaatcggc
    58201 aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg aagtatttga
    58261 tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta atttgcaacg
    58321 agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct tgactggtca
    58381 agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat taccaagtga
    58441 agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac tgatcatttt
    58501 agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa acggtacaca
    58561 ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc caagcatcgc
    58621 gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg gaacagtgaa
    58681 gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact tgcaattaat
    58741 gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc gtgaattagc
    58801 tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct atattactgg
    58861 acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa ctacaagtgc
    58921 agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg ttttacgtga
    58981 tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca gtcggcgaga
    59041 aaagaaaatc atttcaaaac ctcaaattac ttcacgggga ttcgtttacg taaaagctag
    59101 ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga aacatttaga
    59161 aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat taggccgtta
    59221 cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg aagctactca
    59281 acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga gtgctcgtct
    59341 tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc tttataataa
    59401 acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac gttatttaga
    59461 agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag aatctttcac
    59521 agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc tcgtagtgaa
    59581 aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact tagattacaa
    59641 aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg attttgtatt
    59701 aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac tcgcacatcc
    59761 tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa ttgtgtcaag
    59821 cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga tcgtccatcc
    59881 tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga gcagagacct
    59941 caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg ttacaattgc
    60001 tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata ttttttcatt
    60061 gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg cgaggtgtaa
    60121 aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga aaatggccaa
    60181 cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac tgcagaagaa
    60241 gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg agatatgatt
    60301 cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc tgattatgcg
    60361 aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc gtttccattc
    60421 ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga cggtatcgtt
    60481 cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac gatttatcat
    60541 gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt cgatattgat
    60601 gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc agcaaaggct
    60661 gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt ccctgtcatt
    60721 gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa aataatgagc
    60781 gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt tgattttaga
    60841 atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg caaaagaatt
    60901 gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg tcggactagt
    60961 ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa aacatattga
    61021 acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag gcggggcggc
    61081 aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac atgccatgga
    61141 acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag tccaaaaatt
    61201 agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga ctgctgagat
    61261 tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg atacatttac
    61321 agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag atatcatgct
    61381 tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga cagatacgat
    61441 ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag tcgtcaaccg
    61501 ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat cgccaaacca
    61561 gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa tgagtattgc
    61621 ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg tttccgatga
    61681 cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc agcttgtcca
    61741 gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata tccatacgat
    61801 tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg tagtagcacc
    61861 acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa tcatttctga
    61921 tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg aacaagtgaa
    61981 cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc cacgtgtatt
    62041 ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa acaccatcgt
    62101 ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag gagtgaaatg
    62161 acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat tatccacagg
    62221 catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga actattacgg
    62281 gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg gcttcgcttt
    62341 ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt catcgtgact
    62401 gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga aaaactgatc
    62461 aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt aaatacaaaa
    62521 gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga atctttaaag
    62581 atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac agggcgcttt
    62641 ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag aaattatcca
    62701 tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt cgctaaactt
    62761 tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag aataatcctt
    62821 ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc gatcgtttca
    62881 cttcctggag tgattgacga agtgctggct tgggggattt ttgttcagca gccttcaggg
    62941 tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc taaacgccat
    63001 catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc agaagtagag
    63061 gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa aacagaatga
    63121 tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt ttcaagatct
    63181 tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag atgacattac
    63241 caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg ttgatttgcc
    63301 cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa gacggattag
    63361 tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca gtgattaaag
    63421 acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc aagaacatcc
    63481 gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga ccgatgaaac
    63541 tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca cattcttttc
    63601 ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg tatggttatg
    63661 gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta gttatttccg
    63721 gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg agaagcaaaa
    63781 acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag ataaataatg
    63841 tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt aataagatgt
    63901 tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt gtagcagctc
    63961 atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag attcgtgcag
    64021 cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt atggtaggtg
    64081 tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt tctttagcat
    64141 tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga gcaatccaaa
    64201 ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga cacgtattat
    64261 ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag ctgttgcgct
    64321 tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg aaatcagcgc
    64381 tttgatctga gctaataccg agagttagct gattgaatac catttatttg gaa
//
LOCUS       DAZKMZ010000007        62387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000007 DAZKMZ010000000
VERSION     DAZKMZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.007"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACG8M5_000535"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="ACG8M5_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0357817.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACG8M5_000536"
     CDS             complement(1428..1898)
                     /gene="sufU"
                     /locus_tag="ACG8M5_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0357818.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="ACG8M5_000537"
     CDS             complement(1885..3120)
                     /locus_tag="ACG8M5_000537"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0357819.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACG8M5_000538"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="ACG8M5_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0357820.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACG8M5_000539"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="ACG8M5_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0357821.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="ACG8M5_000540"
     CDS             complement(5386..6234)
                     /locus_tag="ACG8M5_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0357822.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="ACG8M5_000541"
     CDS             complement(6258..6944)
                     /locus_tag="ACG8M5_000541"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0357823.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="ACG8M5_000542"
     CDS             complement(6937..7971)
                     /locus_tag="ACG8M5_000542"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357824.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8034..8290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8704)
                     /locus_tag="ACG8M5_000543"
     CDS             complement(8366..8704)
                     /locus_tag="ACG8M5_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0357825.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="ACG8M5_000544"
     CDS             complement(8706..9062)
                     /locus_tag="ACG8M5_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0357826.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="ACG8M5_000545"
     CDS             complement(9068..10255)
                     /locus_tag="ACG8M5_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0357827.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="ACG8M5_000546"
     CDS             complement(10446..11291)
                     /locus_tag="ACG8M5_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0357828.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="ACG8M5_000547"
     CDS             11525..12490
                     /locus_tag="ACG8M5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0357829.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="ACG8M5_000548"
     CDS             12867..13910
                     /locus_tag="ACG8M5_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0357830.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="ACG8M5_000549"
     CDS             complement(13972..14436)
                     /locus_tag="ACG8M5_000549"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0357831.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="ACG8M5_000550"
     CDS             14682..15629
                     /locus_tag="ACG8M5_000550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0357832.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="ACG8M5_000551"
     CDS             complement(15840..17912)
                     /locus_tag="ACG8M5_000551"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0357833.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="ACG8M5_000552"
     CDS             complement(17845..18222)
                     /locus_tag="ACG8M5_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0357834.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18911)
                     /locus_tag="ACG8M5_000553"
     CDS             complement(18351..18911)
                     /locus_tag="ACG8M5_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0357835.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="ACG8M5_000554"
     CDS             19061..20353
                     /locus_tag="ACG8M5_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0357836.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="ACG8M5_000555"
     CDS             complement(20413..21531)
                     /locus_tag="ACG8M5_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0357837.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACG8M5_000556"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="ACG8M5_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0357838.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACG8M5_000557"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="ACG8M5_000557"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0357839.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="ACG8M5_000558"
     CDS             24737..25336
                     /locus_tag="ACG8M5_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0357840.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACG8M5_000559"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="ACG8M5_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0357841.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="ACG8M5_000560"
     CDS             complement(27031..27375)
                     /locus_tag="ACG8M5_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0357842.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28657)
                     /locus_tag="ACG8M5_000561"
     CDS             complement(27512..28657)
                     /locus_tag="ACG8M5_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0357843.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="ACG8M5_000562"
     CDS             complement(28837..29811)
                     /locus_tag="ACG8M5_000562"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0357844.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="ACG8M5_000563"
     CDS             complement(29891..30766)
                     /locus_tag="ACG8M5_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0357845.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="ACG8M5_000564"
     CDS             30896..32803
                     /locus_tag="ACG8M5_000564"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0357846.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="ACG8M5_000565"
     CDS             complement(33344..36622)
                     /locus_tag="ACG8M5_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0357847.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="ACG8M5_000566"
     CDS             complement(36893..37438)
                     /locus_tag="ACG8M5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0357848.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="ACG8M5_000567"
     CDS             complement(37450..38142)
                     /locus_tag="ACG8M5_000567"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0357849.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACG8M5_000568"
     CDS             complement(38161..38445)
                     /gene="macP"
                     /locus_tag="ACG8M5_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0357850.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="ACG8M5_000569"
     CDS             complement(38458..39021)
                     /locus_tag="ACG8M5_000569"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0357851.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="ACG8M5_000570"
     CDS             39249..39911
                     /locus_tag="ACG8M5_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0357852.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="ACG8M5_000571"
     CDS             40035..41231
                     /locus_tag="ACG8M5_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0357853.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="ACG8M5_000572"
     CDS             complement(41332..42708)
                     /locus_tag="ACG8M5_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0357854.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="ACG8M5_000573"
     CDS             complement(42745..43611)
                     /locus_tag="ACG8M5_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0357855.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="ACG8M5_000574"
     CDS             complement(43700..44725)
                     /locus_tag="ACG8M5_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0357856.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="ACG8M5_000575"
     CDS             complement(44743..45417)
                     /locus_tag="ACG8M5_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0357857.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="ACG8M5_000576"
     CDS             complement(45601..46494)
                     /locus_tag="ACG8M5_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0357858.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="ACG8M5_000577"
     CDS             complement(46568..48265)
                     /locus_tag="ACG8M5_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0357859.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="ACG8M5_000578"
     CDS             complement(48258..49571)
                     /locus_tag="ACG8M5_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0357860.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53512)
                     /locus_tag="ACG8M5_000579"
     CDS             complement(49571..53512)
                     /locus_tag="ACG8M5_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0357861.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53621..54079)
                     /locus_tag="ACG8M5_000580"
     CDS             complement(53621..54079)
                     /locus_tag="ACG8M5_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0357862.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54377..54895)
                     /locus_tag="ACG8M5_000581"
     CDS             complement(54377..54895)
                     /locus_tag="ACG8M5_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0357863.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54897..55513)
                     /locus_tag="ACG8M5_000582"
                     /pseudo
     CDS             complement(54897..55513)
                     /locus_tag="ACG8M5_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55510..56298)
                     /locus_tag="ACG8M5_000583"
     CDS             complement(55510..56298)
                     /locus_tag="ACG8M5_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0357864.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(56360..57271)
                     /locus_tag="ACG8M5_000584"
     CDS             complement(56360..57271)
                     /locus_tag="ACG8M5_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0357865.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57312..58646)
                     /locus_tag="ACG8M5_000585"
     CDS             complement(57312..58646)
                     /locus_tag="ACG8M5_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0357866.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58854..59684
                     /locus_tag="ACG8M5_000586"
     CDS             58854..59684
                     /locus_tag="ACG8M5_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0357867.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59928..60177)
                     /locus_tag="ACG8M5_000587"
                     /pseudo
     CDS             complement(59928..60177)
                     /locus_tag="ACG8M5_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60368..60562)
                     /locus_tag="ACG8M5_000588"
     CDS             complement(60368..60562)
                     /locus_tag="ACG8M5_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357868.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(61143..62315)
                     /locus_tag="ACG8M5_000589"
     CDS             complement(61143..62315)
                     /locus_tag="ACG8M5_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0357869.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT        17592 a        13422 c        11249 g        20124 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacggaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    53401 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    53461 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    53521 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    53581 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    53641 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    53701 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    53761 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    53821 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    53881 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    53941 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    54001 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    54061 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    54121 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    54181 tgatatcctt tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta
    54241 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    54301 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    54361 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    54421 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    54481 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    54541 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    54601 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    54661 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    54721 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    54781 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    54841 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    54901 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    54961 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    55021 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    55081 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    55141 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    55201 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    55261 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    55321 gaaccgagct ttttctcttc atttttttgt aatgataaat catatacaga actgttcgac
    55381 ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga
    55441 taagacgtgg aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg
    55501 atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg
    55561 tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata
    55621 attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt
    55681 ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga
    55741 attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat
    55801 atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa
    55861 gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag
    55921 gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt
    55981 tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg
    56041 aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac
    56101 tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat
    56161 cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac
    56221 gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat
    56281 gattcctatt actgtcatga ggacataact tagtattttt ttgtttgccg attcacactg
    56341 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
    56401 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
    56461 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
    56521 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
    56581 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
    56641 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
    56701 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
    56761 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
    56821 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
    56881 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
    56941 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
    57001 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
    57061 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
    57121 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
    57181 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
    57241 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
    57301 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
    57361 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
    57421 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
    57481 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
    57541 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
    57601 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
    57661 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
    57721 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
    57781 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
    57841 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
    57901 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
    57961 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
    58021 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
    58081 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
    58141 aatatctttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
    58201 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
    58261 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
    58321 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
    58381 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
    58441 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
    58501 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
    58561 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
    58621 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
    58681 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
    58741 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
    58801 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
    58861 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
    58921 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
    58981 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
    59041 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
    59101 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
    59161 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
    59221 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
    59281 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
    59341 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
    59401 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
    59461 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
    59521 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
    59581 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
    59641 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
    59701 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
    59761 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
    59821 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
    59881 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
    59941 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
    60001 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
    60061 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
    60121 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
    60181 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
    60241 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
    60301 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
    60361 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
    60421 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
    60481 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
    60541 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
    60601 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
    60661 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
    60721 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
    60781 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
    60841 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
    60901 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
    60961 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
    61021 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
    61081 taaaataacc aagtcaagtc cataatcctg tgtaaaatac tatacaatgt tttaccggtc
    61141 tctcattgat caaacttaat atattttctt gtagaactga gccattcgtc atgaaggtcc
    61201 ataaggacag ctccaattaa tcgattggca gacgttcggt tgggaaaaat ccgaataatc
    61261 ttttctcttc tgcggacttc ctggttcagt cgttcaagaa ggttggtgct ttttagccga
    61321 ttatgactat ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt
    61381 tcgcaggctt ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct
    61441 gttcgagcga gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt
    61501 tttgaattct tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc
    61561 tgccaacttg cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca
    61621 gaaatgatga gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac
    61681 catgtgtcat cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct
    61741 tctgttatcc cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact
    61801 tttatgtaga gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc
    61861 tgccattcgt tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat
    61921 acagattttc cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa
    61981 acatacatct caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac
    62041 accgtcggtg aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca
    62101 cgagtcgtaa agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt
    62161 tcatagtcat cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca
    62221 gtggttaaaa tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc
    62281 tcttcgttgt tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt
    62341 gtggttaaaa acattgtacc gtaaaagggc tgttacatgg cctttta
//
LOCUS       DAZKMZ010000008        53921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000008 DAZKMZ010000000
VERSION     DAZKMZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.008"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(477..980)
                     /locus_tag="ACG8M5_000590"
     CDS             complement(477..980)
                     /locus_tag="ACG8M5_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0357870.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1339..2364
                     /locus_tag="ACG8M5_000591"
     CDS             1339..2364
                     /locus_tag="ACG8M5_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0357871.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2687..3565
                     /locus_tag="ACG8M5_000592"
     CDS             2687..3565
                     /locus_tag="ACG8M5_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0357872.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4041..4157
                     /locus_tag="ACG8M5_000593"
     CDS             4041..4157
                     /locus_tag="ACG8M5_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0357873.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5085..5423
                     /locus_tag="ACG8M5_000594"
     CDS             5085..5423
                     /locus_tag="ACG8M5_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357874.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5517..5747
                     /locus_tag="ACG8M5_000595"
     CDS             5517..5747
                     /locus_tag="ACG8M5_000595"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0357875.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6147..6518
                     /locus_tag="ACG8M5_000596"
     CDS             6147..6518
                     /locus_tag="ACG8M5_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0357876.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6792..7484)
                     /locus_tag="ACG8M5_000597"
     CDS             complement(6792..7484)
                     /locus_tag="ACG8M5_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0357877.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7487..8272)
                     /locus_tag="ACG8M5_000598"
     CDS             complement(7487..8272)
                     /locus_tag="ACG8M5_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0357878.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8509..9909
                     /gene="lacG"
                     /locus_tag="ACG8M5_000599"
     CDS             8509..9909
                     /gene="lacG"
                     /locus_tag="ACG8M5_000599"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0357879.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9934..10251
                     /locus_tag="ACG8M5_000600"
     CDS             9934..10251
                     /locus_tag="ACG8M5_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0357880.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10264..11937
                     /locus_tag="ACG8M5_000601"
     CDS             10264..11937
                     /locus_tag="ACG8M5_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0357881.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11951..13582
                     /locus_tag="ACG8M5_000602"
     CDS             11951..13582
                     /locus_tag="ACG8M5_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0357882.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14073..15638)
                     /gene="guaA"
                     /locus_tag="ACG8M5_000603"
     CDS             complement(14073..15638)
                     /gene="guaA"
                     /locus_tag="ACG8M5_000603"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0357883.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15697..16620)
                     /gene="coaA"
                     /locus_tag="ACG8M5_000604"
     CDS             complement(15697..16620)
                     /gene="coaA"
                     /locus_tag="ACG8M5_000604"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0357884.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16816..17421
                     /locus_tag="ACG8M5_000605"
     CDS             16816..17421
                     /locus_tag="ACG8M5_000605"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0357885.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17729..18745
                     /locus_tag="ACG8M5_000606"
     CDS             17729..18745
                     /locus_tag="ACG8M5_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0357886.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18886..20187
                     /locus_tag="ACG8M5_000607"
     CDS             18886..20187
                     /locus_tag="ACG8M5_000607"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0357887.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20233..20895
                     /gene="deoC"
                     /locus_tag="ACG8M5_000608"
     CDS             20233..20895
                     /gene="deoC"
                     /locus_tag="ACG8M5_000608"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0357888.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20916..21314
                     /locus_tag="ACG8M5_000609"
     CDS             20916..21314
                     /locus_tag="ACG8M5_000609"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0357889.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21380..22462
                     /locus_tag="ACG8M5_000610"
     CDS             21380..22462
                     /locus_tag="ACG8M5_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0357890.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22674..24236
                     /locus_tag="ACG8M5_000611"
     CDS             22674..24236
                     /locus_tag="ACG8M5_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357891.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24229..25362
                     /locus_tag="ACG8M5_000612"
     CDS             24229..25362
                     /locus_tag="ACG8M5_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357892.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25362..26318
                     /locus_tag="ACG8M5_000613"
     CDS             25362..26318
                     /locus_tag="ACG8M5_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357893.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26417..27583
                     /gene="deoB"
                     /locus_tag="ACG8M5_000614"
     CDS             26417..27583
                     /gene="deoB"
                     /locus_tag="ACG8M5_000614"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0357894.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27607..28425
                     /locus_tag="ACG8M5_000615"
     CDS             27607..28425
                     /locus_tag="ACG8M5_000615"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0357895.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28442..29149
                     /gene="deoD"
                     /locus_tag="ACG8M5_000616"
     CDS             28442..29149
                     /gene="deoD"
                     /locus_tag="ACG8M5_000616"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0357896.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29447..30133)
                     /gene="gpmA"
                     /locus_tag="ACG8M5_000617"
     CDS             complement(29447..30133)
                     /gene="gpmA"
                     /locus_tag="ACG8M5_000617"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0357897.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30305..30886)
                     /locus_tag="ACG8M5_000618"
     CDS             complement(30305..30886)
                     /locus_tag="ACG8M5_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0357898.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31019..31702)
                     /gene="rpiA"
                     /locus_tag="ACG8M5_000619"
     CDS             complement(31019..31702)
                     /gene="rpiA"
                     /locus_tag="ACG8M5_000619"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0357899.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32021..32434
                     /gene="rpsL"
                     /locus_tag="ACG8M5_000620"
     CDS             32021..32434
                     /gene="rpsL"
                     /locus_tag="ACG8M5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0357900.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32518..32988
                     /gene="rpsG"
                     /locus_tag="ACG8M5_000621"
     CDS             32518..32988
                     /gene="rpsG"
                     /locus_tag="ACG8M5_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0357901.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33082..35169
                     /gene="fusA"
                     /locus_tag="ACG8M5_000622"
     CDS             33082..35169
                     /gene="fusA"
                     /locus_tag="ACG8M5_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0357902.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35341..36528
                     /gene="tuf"
                     /locus_tag="ACG8M5_000623"
     CDS             35341..36528
                     /gene="tuf"
                     /locus_tag="ACG8M5_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0357903.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36935..37243
                     /gene="rpsJ"
                     /locus_tag="ACG8M5_000624"
     CDS             36935..37243
                     /gene="rpsJ"
                     /locus_tag="ACG8M5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0357904.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37274..37903
                     /gene="rplC"
                     /locus_tag="ACG8M5_000625"
     CDS             37274..37903
                     /gene="rplC"
                     /locus_tag="ACG8M5_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0357905.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37931..38554
                     /gene="rplD"
                     /locus_tag="ACG8M5_000626"
     CDS             37931..38554
                     /gene="rplD"
                     /locus_tag="ACG8M5_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0357906.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38554..38844
                     /gene="rplW"
                     /locus_tag="ACG8M5_000627"
     CDS             38554..38844
                     /gene="rplW"
                     /locus_tag="ACG8M5_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0357907.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38883..39716
                     /gene="rplB"
                     /locus_tag="ACG8M5_000628"
     CDS             38883..39716
                     /gene="rplB"
                     /locus_tag="ACG8M5_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0357908.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39759..40037
                     /gene="rpsS"
                     /locus_tag="ACG8M5_000629"
     CDS             39759..40037
                     /gene="rpsS"
                     /locus_tag="ACG8M5_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0357909.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40060..40407
                     /gene="rplV"
                     /locus_tag="ACG8M5_000630"
     CDS             40060..40407
                     /gene="rplV"
                     /locus_tag="ACG8M5_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0357910.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40421..41077
                     /gene="rpsC"
                     /locus_tag="ACG8M5_000631"
     CDS             40421..41077
                     /gene="rpsC"
                     /locus_tag="ACG8M5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0357911.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41080..41514
                     /gene="rplP"
                     /locus_tag="ACG8M5_000632"
     CDS             41080..41514
                     /gene="rplP"
                     /locus_tag="ACG8M5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0357912.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41504..41692
                     /gene="rpmC"
                     /locus_tag="ACG8M5_000633"
     CDS             41504..41692
                     /gene="rpmC"
                     /locus_tag="ACG8M5_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0357913.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41717..41983
                     /gene="rpsQ"
                     /locus_tag="ACG8M5_000634"
     CDS             41717..41983
                     /gene="rpsQ"
                     /locus_tag="ACG8M5_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0357914.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42041..42409
                     /gene="rplN"
                     /locus_tag="ACG8M5_000635"
     CDS             42041..42409
                     /gene="rplN"
                     /locus_tag="ACG8M5_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0357915.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42446..42754
                     /gene="rplX"
                     /locus_tag="ACG8M5_000636"
     CDS             42446..42754
                     /gene="rplX"
                     /locus_tag="ACG8M5_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0357916.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42782..43321
                     /gene="rplE"
                     /locus_tag="ACG8M5_000637"
     CDS             42782..43321
                     /gene="rplE"
                     /locus_tag="ACG8M5_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0357917.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43340..43525
                     /locus_tag="ACG8M5_000638"
     CDS             43340..43525
                     /locus_tag="ACG8M5_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0357918.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43561..43959
                     /gene="rpsH"
                     /locus_tag="ACG8M5_000639"
     CDS             43561..43959
                     /gene="rpsH"
                     /locus_tag="ACG8M5_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0357919.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43991..44527
                     /gene="rplF"
                     /locus_tag="ACG8M5_000640"
     CDS             43991..44527
                     /gene="rplF"
                     /locus_tag="ACG8M5_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0357920.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44688..45044
                     /gene="rplR"
                     /locus_tag="ACG8M5_000641"
     CDS             44688..45044
                     /gene="rplR"
                     /locus_tag="ACG8M5_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0357921.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45065..45565
                     /gene="rpsE"
                     /locus_tag="ACG8M5_000642"
     CDS             45065..45565
                     /gene="rpsE"
                     /locus_tag="ACG8M5_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0357922.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45580..45759
                     /gene="rpmD"
                     /locus_tag="ACG8M5_000643"
     CDS             45580..45759
                     /gene="rpmD"
                     /locus_tag="ACG8M5_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0357923.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45803..46243
                     /gene="rplO"
                     /locus_tag="ACG8M5_000644"
     CDS             45803..46243
                     /gene="rplO"
                     /locus_tag="ACG8M5_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0357924.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46243..47538
                     /gene="secY"
                     /locus_tag="ACG8M5_000645"
     CDS             46243..47538
                     /gene="secY"
                     /locus_tag="ACG8M5_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0357925.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47600..48247
                     /locus_tag="ACG8M5_000646"
     CDS             47600..48247
                     /locus_tag="ACG8M5_000646"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0357926.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48438..48656
                     /gene="infA"
                     /locus_tag="ACG8M5_000647"
     CDS             48438..48656
                     /gene="infA"
                     /locus_tag="ACG8M5_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0357927.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48689..48805
                     /gene="rpmJ"
                     /locus_tag="ACG8M5_000648"
     CDS             48689..48805
                     /gene="rpmJ"
                     /locus_tag="ACG8M5_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0357928.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48823..49188
                     /gene="rpsM"
                     /locus_tag="ACG8M5_000649"
     CDS             48823..49188
                     /gene="rpsM"
                     /locus_tag="ACG8M5_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0357929.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49216..49605
                     /gene="rpsK"
                     /locus_tag="ACG8M5_000650"
     CDS             49216..49605
                     /gene="rpsK"
                     /locus_tag="ACG8M5_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0357930.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49685..50623
                     /locus_tag="ACG8M5_000651"
     CDS             49685..50623
                     /locus_tag="ACG8M5_000651"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0357931.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50651..51031
                     /gene="rplQ"
                     /locus_tag="ACG8M5_000652"
     CDS             50651..51031
                     /gene="rplQ"
                     /locus_tag="ACG8M5_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0357932.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51303..52142
                     /locus_tag="ACG8M5_000653"
     CDS             51303..52142
                     /locus_tag="ACG8M5_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0357933.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52118..52987
                     /locus_tag="ACG8M5_000654"
     CDS             52118..52987
                     /locus_tag="ACG8M5_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0357934.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52977..53774
                     /locus_tag="ACG8M5_000655"
     CDS             52977..53774
                     /locus_tag="ACG8M5_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0357935.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        17188 a         9663 c        11605 g        15465 t
ORIGIN      
        1 acgacacaga aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt
       61 tcctttgaaa tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga
      121 agggagccta aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag
      181 ctttttatag aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta
      241 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
      301 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
      361 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
      421 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
      481 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
      541 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
      601 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
      661 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
      721 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
      781 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
      841 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
      901 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
      961 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
     1021 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
     1081 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
     1141 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
     1201 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     1261 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     1321 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     1381 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     1441 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     1501 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     1561 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     1621 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     1681 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     1741 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     1801 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     1861 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     1921 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
     1981 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
     2041 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
     2101 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
     2161 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
     2221 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
     2281 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
     2341 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
     2401 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
     2461 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
     2521 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
     2581 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
     2641 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
     2701 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
     2761 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
     2821 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
     2881 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
     2941 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
     3001 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
     3061 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
     3121 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
     3181 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
     3241 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
     3301 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
     3361 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
     3421 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
     3481 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
     3541 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
     3601 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     3661 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     3721 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga
     3781 acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca
     3841 agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag
     3901 tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac
     3961 catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa
     4021 gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga
     4081 aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga
     4141 atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt
     4201 cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc
     4261 catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa
     4321 ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct
     4381 gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta
     4441 ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac
     4501 ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg
     4561 ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt
     4621 cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc
     4681 gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt
     4741 actattgcta gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa
     4801 gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat
     4861 cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta
     4921 aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt
     4981 tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga
     5041 ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt
     5101 caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa
     5161 atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa
     5221 taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata
     5281 cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata
     5341 ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata
     5401 aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga
     5461 aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc
     5521 tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg
     5581 aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag
     5641 tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt
     5701 tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt
     5761 atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta
     5821 ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta
     5881 gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt
     5941 ttcttaatta gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt
     6001 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
     6061 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
     6121 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
     6181 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
     6241 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
     6301 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
     6361 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
     6421 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
     6481 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
     6541 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
     6601 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
     6661 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
     6721 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
     6781 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
     6841 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
     6901 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
     6961 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
     7021 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
     7081 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
     7141 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
     7201 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
     7261 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
     7321 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
     7381 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
     7441 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
     7501 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
     7561 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
     7621 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
     7681 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
     7741 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
     7801 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
     7861 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
     7921 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
     7981 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
     8041 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
     8101 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
     8161 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
     8221 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
     8281 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
     8341 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
     8401 ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
     8461 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
     8521 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
     8581 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
     8641 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
     8701 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
     8761 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
     8821 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
     8881 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
     8941 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
     9001 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
     9061 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
     9121 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
     9181 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
     9241 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
     9301 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
     9361 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
     9421 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
     9481 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
     9541 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
     9601 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
     9661 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
     9721 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
     9781 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
     9841 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
     9901 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
     9961 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
    10021 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
    10081 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
    10141 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
    10201 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
    10261 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
    10321 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
    10381 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
    10441 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
    10501 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
    10561 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
    10621 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
    10681 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
    10741 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
    10801 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
    10861 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
    10921 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
    10981 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
    11041 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
    11101 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
    11161 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
    11221 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
    11281 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
    11341 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
    11401 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
    11461 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
    11521 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
    11581 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
    11641 gtactttgtg ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca
    11701 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
    11761 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
    11821 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
    11881 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
    11941 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
    12001 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
    12061 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
    12121 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
    12181 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
    12241 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
    12301 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
    12361 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
    12421 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
    12481 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
    12541 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
    12601 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
    12661 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
    12721 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
    12781 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
    12841 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
    12901 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
    12961 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
    13021 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
    13081 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
    13141 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
    13201 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
    13261 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
    13321 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
    13381 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
    13441 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
    13501 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
    13561 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
    13621 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
    13681 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
    13741 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
    13801 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
    13861 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
    13921 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
    13981 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
    14041 tagggattcc ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt
    14101 ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca
    14161 gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt
    14221 gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg
    14281 acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca
    14341 gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca
    14401 gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg
    14461 attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg
    14521 tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga
    14581 gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct
    14641 tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca
    14701 tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac
    14761 ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct
    14821 agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata
    14881 gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg
    14941 ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact
    15001 tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca
    15061 aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata
    15121 ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg
    15181 actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt
    15241 tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct
    15301 ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat
    15361 tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc
    15421 ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga
    15481 ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca
    15541 agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata
    15601 atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta
    15661 aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga
    15721 atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa
    15781 gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa
    15841 ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc
    15901 cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc
    15961 atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa
    16021 ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt
    16081 gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc
    16141 agattttaca cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc
    16201 tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt
    16261 cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct
    16321 ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac
    16381 ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat
    16441 ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat
    16501 gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt
    16561 acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat
    16621 attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc
    16681 caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag
    16741 tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg
    16801 agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat
    16861 tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt
    16921 gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg
    16981 gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg
    17041 ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg
    17101 gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa
    17161 tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg
    17221 atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag
    17281 aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag
    17341 aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc
    17401 atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg
    17461 ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag
    17521 aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat
    17581 aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat
    17641 caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt acactgatag
    17701 ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat
    17761 ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag
    17821 tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta
    17881 tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt
    17941 cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg
    18001 ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca
    18061 agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga
    18121 gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg
    18181 attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc
    18241 tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg
    18301 atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct
    18361 gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc
    18421 atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat
    18481 ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc
    18541 agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt
    18601 gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga
    18661 atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag
    18721 cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa
    18781 ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc
    18841 taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat
    18901 ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc
    18961 acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt
    19021 ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct
    19081 ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt
    19141 ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca
    19201 gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa
    19261 gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa
    19321 agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat
    19381 gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg
    19441 agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt
    19501 gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg
    19561 aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa
    19621 gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca
    19681 gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa
    19741 gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg
    19801 ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat
    19861 ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt
    19921 gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt
    19981 aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt
    20041 gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact
    20101 tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg
    20161 ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa
    20221 aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca
    20281 tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc
    20341 tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc
    20401 gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa
    20461 acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct
    20521 ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca
    20581 aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc
    20641 atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt
    20701 ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca
    20761 gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc
    20821 gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact
    20881 ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga
    20941 tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag
    21001 gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat
    21061 catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag
    21121 aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg
    21181 gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag
    21241 gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg
    21301 aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat
    21361 tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact
    21421 agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg
    21481 caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga
    21541 ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact
    21601 ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc
    21661 aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg gttataaact
    21721 aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga
    21781 tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc
    21841 ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg
    21901 cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc
    21961 aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc
    22021 tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat
    22081 cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga
    22141 atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga
    22201 atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg
    22261 aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca
    22321 cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga
    22381 agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc
    22441 agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa
    22501 ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg
    22561 gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt
    22621 gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc
    22681 aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa
    22741 atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg
    22801 gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga
    22861 aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag
    22921 ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca
    22981 tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag
    23041 agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg
    23101 atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag
    23161 atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa
    23221 tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt
    23281 tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg
    23341 gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa
    23401 cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg
    23461 aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg
    23521 ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga
    23581 tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag
    23641 acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag
    23701 accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa
    23761 cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac
    23821 atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa
    23881 aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc
    23941 ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta
    24001 ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg
    24061 aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg
    24121 tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct
    24181 ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc
    24241 agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta
    24301 ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta
    24361 gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg
    24421 atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt
    24481 tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat
    24541 ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg
    24601 gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca
    24661 atcatgatga actatatctt attatacgta agcacatttg ctttacatga agtgatgcct
    24721 gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg
    24781 ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca
    24841 atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc
    24901 cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg
    24961 atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga
    25021 ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga
    25081 atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg
    25141 ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag
    25201 atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt
    25261 ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa
    25321 tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa
    25381 ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta acgtcactag
    25441 gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca
    25501 tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga
    25561 caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg
    25621 cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga
    25681 tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata
    25741 acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg
    25801 gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc
    25861 tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa
    25921 atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac
    25981 tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta
    26041 acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg
    26101 gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct
    26161 tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa
    26221 ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc
    26281 caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt
    26341 gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa
    26401 gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat
    26461 tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat
    26521 tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg
    26581 tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga
    26641 agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa
    26701 aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga
    26761 attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga
    26821 ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt
    26881 ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca
    26941 attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc
    27001 gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa
    27061 tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt
    27121 tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag
    27181 caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag
    27241 ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta
    27301 tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga
    27361 agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga
    27421 tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc
    27481 attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc
    27541 ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga
    27601 aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa
    27661 ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat
    27721 gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca
    27781 gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc
    27841 atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta
    27901 cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc
    27961 aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt
    28021 gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt
    28081 caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg
    28141 gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag
    28201 atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc
    28261 atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca
    28321 gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa
    28381 caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa
    28441 aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg
    28501 agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa
    28561 tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca
    28621 aggaacagga atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta
    28681 tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt
    28741 tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt
    28801 ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat
    28861 agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta
    28921 caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat
    28981 ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat
    29041 gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac
    29101 gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga
    29161 aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt
    29221 gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg
    29281 tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    29341 tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa
    29401 agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt
    29461 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
    29521 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
    29581 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
    29641 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
    29701 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
    29761 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
    29821 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
    29881 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
    29941 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
    30001 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
    30061 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
    30121 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
    30181 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
    30241 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
    30301 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
    30361 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
    30421 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
    30481 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
    30541 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
    30601 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
    30661 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
    30721 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
    30781 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
    30841 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
    30901 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
    30961 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
    31021 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
    31081 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
    31141 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
    31201 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
    31261 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
    31321 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
    31381 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
    31441 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
    31501 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
    31561 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
    31621 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
    31681 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
    31741 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
    31801 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
    31861 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
    31921 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
    31981 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
    32041 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
    32101 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
    32161 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
    32221 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
    32281 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
    32341 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
    32401 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
    32461 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
    32521 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
    32581 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
    32641 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
    32701 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
    32761 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
    32821 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
    32881 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
    32941 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
    33001 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
    33061 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
    33121 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
    33181 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
    33241 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
    33301 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
    33361 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
    33421 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
    33481 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
    33541 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
    33601 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
    33661 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
    33721 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
    33781 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
    33841 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
    33901 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
    33961 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
    34021 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
    34081 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
    34141 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
    34201 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
    34261 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
    34321 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
    34381 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
    34441 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
    34501 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
    34561 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
    34621 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
    34681 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
    34741 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
    34801 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
    34861 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
    34921 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
    34981 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
    35041 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
    35101 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
    35161 gctgaataat ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
    35221 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
    35281 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
    35341 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
    35401 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
    35461 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
    35521 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
    35581 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
    35641 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
    35701 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
    35761 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
    35821 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
    35881 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
    35941 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
    36001 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
    36061 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
    36121 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
    36181 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
    36241 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
    36301 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    36361 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
    36421 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
    36481 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
    36541 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
    36601 gttccgagct tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga
    36661 agaaacacga aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt
    36721 ttttacaaga aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt
    36781 tactttagtg aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt
    36841 tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg
    36901 tctacttttc aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg
    36961 tttaaaagcg tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc
    37021 aaaaagaact ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta
    37081 cacaatcatc cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac
    37141 acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa
    37201 gctagactta ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa
    37261 tggaggtgta ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt
    37321 cactgaatct ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact
    37381 acaagtgaaa acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat
    37441 gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc
    37501 taagcgcttc attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga
    37561 aattacagta gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg
    37621 taaaggattc caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg
    37681 ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa
    37741 aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt
    37801 tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa
    37861 aaaatcttta attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag
    37921 gaactaagga atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat
    37981 cacacttaac gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat
    38041 cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt
    38101 tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc
    38161 tatccgctca ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta
    38221 cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa
    38281 agttgctgaa aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa
    38341 agaattcaaa caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga
    38401 aaaaggtaac gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac
    38461 ttctgataat gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac
    38521 tgctcttact caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc
    38581 ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg
    38641 gacactcgcg caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa
    38701 gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca
    38761 ggatatacaa aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc
    38821 caattattcg aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa
    38881 acgtggcgat taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg
    38941 attttgctga aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc
    39001 atgctggacg taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac
    39061 gccaatatcg cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa
    39121 cgatcgaata tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag
    39181 tgaaagctta catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc
    39241 cagaagcaga tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg
    39301 ttatccacaa cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta
    39361 caagtgctca agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg
    39421 aagttcgtat gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac
    39481 acgaattaat caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg
    39541 tacgtggtag cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc
    39601 ctatcggacg taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc
    39661 gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac
    39721 tttgaccatg tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag
    39781 gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga
    39841 aaaaagttat caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata
    39901 ccatcgcagt ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag
    39961 gacacaaatt aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata
    40021 agaaaacgaa acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca
    40081 gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac
    40141 cttatcagag gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa
    40201 tctgctggaa tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt
    40261 gacttagatg ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg
    40321 aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat
    40381 atcacggtag tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc
    40441 aattggaatg cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga
    40501 gtatgctgag ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc
    40561 tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat
    40621 tcacacagct aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag
    40681 aaaagaatta aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa
    40741 accagatcta gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt
    40801 tgctttccgt cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg
    40861 gatcaaaact caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata
    40921 ctcagaagga actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga
    40981 agcagataca acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct
    41041 tccaactaaa aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta
    41101 aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta
    41161 gcattcggtg aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc
    41221 gaagctgctc gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa
    41281 attttccctc acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa
    41341 ggggcacctg aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca
    41401 ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg
    41461 aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat
    41521 cagagaatta accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt
    41581 taatcttaga ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt
    41641 acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa
    41701 ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt
    41761 ggtttcagac aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc
    41821 tatctatggc aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac
    41881 agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg
    41941 tttccgtttg ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct
    42001 atatagattg aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg
    42061 tttaaaagtc gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg
    42121 atctggtcgt aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac
    42181 gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc
    42241 aggagctcgt cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat
    42301 tcgtgatgat aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg
    42361 tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac
    42421 ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat
    42481 cactggaaaa gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa
    42541 agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc
    42601 tcaaggtgga attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga
    42661 ctctactggt gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt
    42721 ttctaaaaaa accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg
    42781 aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt
    42841 taactatagc tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt
    42901 tggtgatgcg gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat
    42961 cactggtcaa aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg
    43021 tgaaggaatg ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt
    43081 agacaaattg gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa
    43141 agcctttgac ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga
    43201 agtagattat gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc
    43261 aaactctgat gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata
    43321 attaaaggag gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc
    43381 aaaattttca acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta
    43441 tcgtaagttc catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc
    43501 cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca
    43561 atggtcatga cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt
    43621 aaacatgagt ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg
    43681 aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc
    43741 cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc
    43801 tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt
    43861 ttaggtatcg caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa
    43921 aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag
    43981 gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt
    44041 tactcaagac ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt
    44101 ttcagcagat atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga
    44161 cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt
    44221 aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca
    44281 attacaagga tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc
    44341 accagctgga gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa
    44401 caaagaagaa gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta
    44461 taaaggtaaa ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg
    44521 taaataatcg tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga
    44581 agtttacctt caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta
    44641 tttactgctt gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac
    44701 cagataagaa caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta
    44761 ctgctgagcg cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta
    44821 ttgatgacgt agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag
    44881 gtggaacaaa aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg
    44941 aaaaaggcat taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc
    45001 aagctttagc tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc
    45061 attcatggtt tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa
    45121 ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt
    45181 cggtgacaaa aacggacacg taggatttgg tacaggtaaa gctcaagaag ttcctgaagc
    45241 aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc
    45301 aacaatccca cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc
    45361 tgtagaaggt tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg
    45421 ggtagcagat atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc
    45481 aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa
    45541 atcagttgaa gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact
    45601 ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt
    45661 cttacaaaga ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc
    45721 aatactgttt ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga
    45781 acattaagga ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg
    45841 ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg
    45901 cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg
    45961 acaaacacca ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga
    46021 ctatgcagtc attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc
    46081 tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc
    46141 taatggggaa ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca
    46201 agaagcaatt gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa
    46261 gatgctttta aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt
    46321 gtatttcgcc taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac
    46381 ctttcttctt tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac
    46441 ttttccattt tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt
    46501 cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt
    46561 aaaaaactga atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc
    46621 ggtatcactg ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta
    46681 gcaacttacg taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta
    46741 ggagaacaga tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt
    46801 atcatttcta gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt
    46861 gatccttcag acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg
    46921 ttgatcgtga cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat
    46981 acgaaacgcg tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct
    47041 ggagttattc cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa
    47101 gcattgagcg gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac
    47161 tataatacag cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc
    47221 tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt
    47281 tatataccaa gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg
    47341 cgtttaagta cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct
    47401 caaatggttt gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt
    47461 atcggtgtag ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat
    47521 gttggattca ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca
    47581 tttattagga gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg
    47641 aacacaagct gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat
    47701 gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga
    47761 taaaggtgca ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga
    47821 accagataca gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa
    47881 agctctggac gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca
    47941 tgttggtgaa gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg
    48001 agcaacttac cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg
    48061 tgggcatgaa ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc
    48121 tatcaacatc aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa
    48181 cactattgat ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga
    48241 aaaatagcac gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat
    48301 aaattatgat agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga
    48361 gaattttgag gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt
    48421 acaaggaggt actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg
    48481 aaactttgcc gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta
    48541 ctgtttcagg taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg
    48601 ttgaattgtc tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt
    48661 actccgtaaa actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg
    48721 cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc
    48781 aaaacataaa caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga
    48841 gtagatattc ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt
    48901 aacactactg ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt
    48961 gaattaacga atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa
    49021 ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac
    49081 cgtggaatcc gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca
    49141 cgtactcgta aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa
    49201 ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat
    49261 atagaagcag gtattgcaca tatccattct acattcaata acactatcat catgatcact
    49321 gatactcacg gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc
    49381 aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg
    49441 gaacacggat taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca
    49501 gcaattcgtt cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca
    49561 gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt
    49621 ttgagtttta tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat
    49681 aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg
    49741 caagttcatc gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg
    49801 tcgtatttta ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt
    49861 actgcatgaa ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa
    49921 cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat
    49981 taccggacct gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct
    50041 taacaaagat atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac
    50101 agtgaaacct ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat
    50161 tggcgttctt ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga
    50221 gaacacacgt gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga
    50281 tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt
    50341 ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga
    50401 agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg
    50461 ctcttacaat tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc
    50521 tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc
    50581 aaaattgcac gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag
    50641 ggaaaccaac gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt
    50701 gcgtgattta acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc
    50761 gaaagaagtt cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca
    50821 tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa
    50881 tgaggacatc gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta
    50941 cgcagaacgt caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg
    51001 tgcaccaatg gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat
    51061 gattctttta gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct
    51121 cctcaagcga atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc
    51181 aattacctgt catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa
    51241 ttgaaaattt tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa
    51301 tcatggagcc aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc
    51361 gccccgcatt gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg
    51421 gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag
    51481 aatcagggat cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc
    51541 gacagatggt tggaatggtt tttcaaaatc cagacaatca gtttgttggt tcgacagtag
    51601 aagatgacgt tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc
    51661 gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc
    51721 gtttatcagg agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg
    51781 atatcattat tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga
    51841 tctctacgat caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg
    51901 atattgatga agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc
    51961 gtgaaggtac accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag
    52021 atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca
    52081 gtgaatatat gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt
    52141 agacttcact tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat
    52201 gacgatcaaa gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac
    52261 cttacttcag catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga
    52321 acgagatatt cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg
    52381 catcgtcttt cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc
    52441 tttcggtcct aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac
    52501 attagaacaa gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg
    52561 agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt
    52621 attggatgaa ccaacggcag gacttgatcc ccagggcaga aaagaaatga tggagatgtt
    52681 ttggcgtctt cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga
    52741 tgtagccaat tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg
    52801 ggagccgcaa gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc
    52861 tactgccacc gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc
    52921 tttgacagct gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga
    52981 tgaataaatt gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc
    53041 cccgagctaa attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt
    53101 gggaaacgta ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag
    53161 atttgaaatt ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag
    53221 ctttgcaggt cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata
    53281 tcactgaatt tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt
    53341 ttatgtcgac tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt
    53401 acttgcttcg tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt
    53461 cgattgctct tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc
    53521 agcgcgctcg cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca
    53581 ttcctttgct gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg
    53641 ctatggaagc aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt
    53701 ggcataatag agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct
    53761 ttttaagagg ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc
    53821 aagaaaggat gcaagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca
    53881 caggcctctt tcttttatct gaaaagaaag aacgaaaaaa t
//
LOCUS       DAZKMZ010000009        53719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000009 DAZKMZ010000000
VERSION     DAZKMZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.009"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..840
                     /locus_tag="ACG8M5_000656"
     CDS             160..840
                     /locus_tag="ACG8M5_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0357936.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACG8M5_000657"
     CDS             930..1964
                     /locus_tag="ACG8M5_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0357937.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACG8M5_000658"
     CDS             1978..2313
                     /locus_tag="ACG8M5_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357938.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACG8M5_000659"
     CDS             2297..4777
                     /locus_tag="ACG8M5_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357939.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACG8M5_000660"
     CDS             4833..6392
                     /locus_tag="ACG8M5_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0357940.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACG8M5_000661"
     CDS             6389..7753
                     /locus_tag="ACG8M5_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0357941.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACG8M5_000662"
     CDS             7924..8994
                     /locus_tag="ACG8M5_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357942.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACG8M5_000663"
     CDS             9009..9677
                     /locus_tag="ACG8M5_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357943.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACG8M5_000664"
     CDS             9697..10275
                     /locus_tag="ACG8M5_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0357944.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACG8M5_000665"
     CDS             10676..11587
                     /locus_tag="ACG8M5_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0357945.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG8M5_000666"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACG8M5_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0357946.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACG8M5_000667"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACG8M5_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0357947.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACG8M5_000668"
     CDS             13579..14127
                     /locus_tag="ACG8M5_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0357948.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACG8M5_000669"
     CDS             14242..16602
                     /locus_tag="ACG8M5_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0357949.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACG8M5_000670"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACG8M5_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0357950.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG8M5_000671"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACG8M5_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0357951.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACG8M5_000672"
     CDS             19108..20007
                     /locus_tag="ACG8M5_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357952.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACG8M5_000673"
     CDS             20000..21262
                     /locus_tag="ACG8M5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357953.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACG8M5_000674"
     CDS             21423..23321
                     /locus_tag="ACG8M5_000674"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0357954.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACG8M5_000675"
     CDS             complement(23651..24370)
                     /locus_tag="ACG8M5_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0357955.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACG8M5_000676"
     CDS             24707..25039
                     /locus_tag="ACG8M5_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0357956.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACG8M5_000677"
     CDS             25026..27029
                     /locus_tag="ACG8M5_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0357957.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACG8M5_000678"
     CDS             27048..27518
                     /locus_tag="ACG8M5_000678"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0357958.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACG8M5_000679"
     CDS             27539..28123
                     /locus_tag="ACG8M5_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0357959.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACG8M5_000680"
     CDS             28138..29124
                     /locus_tag="ACG8M5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0357960.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACG8M5_000681"
     CDS             29114..29425
                     /locus_tag="ACG8M5_000681"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0357961.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACG8M5_000682"
     CDS             29447..31228
                     /locus_tag="ACG8M5_000682"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0357962.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACG8M5_000683"
     CDS             31221..32597
                     /locus_tag="ACG8M5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0357963.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACG8M5_000684"
     CDS             32604..33239
                     /locus_tag="ACG8M5_000684"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0357964.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACG8M5_000685"
     CDS             33269..34624
                     /locus_tag="ACG8M5_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0357965.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACG8M5_000686"
     CDS             complement(34816..36210)
                     /locus_tag="ACG8M5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0357966.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACG8M5_000687"
     CDS             36302..37273
                     /locus_tag="ACG8M5_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0357967.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACG8M5_000688"
     CDS             37270..38052
                     /locus_tag="ACG8M5_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0357968.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACG8M5_000689"
     CDS             complement(38221..38592)
                     /locus_tag="ACG8M5_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0357969.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACG8M5_000690"
     CDS             38726..39169
                     /locus_tag="ACG8M5_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0357970.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG8M5_000691"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACG8M5_000691"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0357971.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACG8M5_000692"
     CDS             complement(40040..41407)
                     /locus_tag="ACG8M5_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0357972.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACG8M5_000693"
     CDS             complement(41391..42221)
                     /locus_tag="ACG8M5_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0357973.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACG8M5_000694"
     CDS             42384..42821
                     /locus_tag="ACG8M5_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0357974.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG8M5_000695"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACG8M5_000695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0357975.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACG8M5_000696"
     CDS             43706..44212
                     /locus_tag="ACG8M5_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0357976.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACG8M5_000697"
     CDS             44229..45128
                     /locus_tag="ACG8M5_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0357977.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACG8M5_000698"
     CDS             45215..46099
                     /locus_tag="ACG8M5_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0357978.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="ACG8M5_000699"
     tRNA            complement(46291..46362)
                     /locus_tag="ACG8M5_000699"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG8M5_000700"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="ACG8M5_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0357979.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="ACG8M5_000701"
     CDS             47905..48996
                     /locus_tag="ACG8M5_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0357980.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="ACG8M5_000702"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="ACG8M5_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0357981.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /gene="pgk"
                     /locus_tag="ACG8M5_000703"
     CDS             50148..51338
                     /gene="pgk"
                     /locus_tag="ACG8M5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0357982.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG8M5_000704"
     CDS             51442..52197
                     /gene="tpiA"
                     /locus_tag="ACG8M5_000704"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0357983.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="ACG8M5_000705"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="ACG8M5_000705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0357984.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17827 a         9355 c        11351 g        15186 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttagttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgacttgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagt
//
LOCUS       DAZKMZ010000010        50926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000010 DAZKMZ010000000
VERSION     DAZKMZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.010"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..891
                     /locus_tag="ACG8M5_000706"
     CDS             46..891
                     /locus_tag="ACG8M5_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0357985.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1172..2392
                     /locus_tag="ACG8M5_000707"
     CDS             1172..2392
                     /locus_tag="ACG8M5_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0357986.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2389..5109
                     /locus_tag="ACG8M5_000708"
     CDS             2389..5109
                     /locus_tag="ACG8M5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0357987.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5093..6037
                     /locus_tag="ACG8M5_000709"
     CDS             5093..6037
                     /locus_tag="ACG8M5_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0357988.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6106..7116)
                     /locus_tag="ACG8M5_000710"
     CDS             complement(6106..7116)
                     /locus_tag="ACG8M5_000710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0357989.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7296..7640)
                     /locus_tag="ACG8M5_000711"
     CDS             complement(7296..7640)
                     /locus_tag="ACG8M5_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0357990.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7633..8067)
                     /locus_tag="ACG8M5_000712"
     CDS             complement(7633..8067)
                     /locus_tag="ACG8M5_000712"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0357991.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8205..8942
                     /locus_tag="ACG8M5_000713"
     CDS             8205..8942
                     /locus_tag="ACG8M5_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0357992.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8935..10146
                     /locus_tag="ACG8M5_000714"
     CDS             8935..10146
                     /locus_tag="ACG8M5_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0357993.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10350..11132
                     /locus_tag="ACG8M5_000715"
     CDS             10350..11132
                     /locus_tag="ACG8M5_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0357994.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11202..11864
                     /gene="trmB"
                     /locus_tag="ACG8M5_000716"
     CDS             11202..11864
                     /gene="trmB"
                     /locus_tag="ACG8M5_000716"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0357995.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11962..13716)
                     /gene="ade"
                     /locus_tag="ACG8M5_000717"
     CDS             complement(11962..13716)
                     /gene="ade"
                     /locus_tag="ACG8M5_000717"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0357996.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13848..14777
                     /gene="comGA"
                     /locus_tag="ACG8M5_000718"
     CDS             13848..14777
                     /gene="comGA"
                     /locus_tag="ACG8M5_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0357997.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14734..15783
                     /gene="comGB"
                     /locus_tag="ACG8M5_000719"
     CDS             14734..15783
                     /gene="comGB"
                     /locus_tag="ACG8M5_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0357998.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15780..16058
                     /gene="comGC"
                     /locus_tag="ACG8M5_000720"
     CDS             15780..16058
                     /gene="comGC"
                     /locus_tag="ACG8M5_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0357999.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16033..16503
                     /gene="comGD"
                     /locus_tag="ACG8M5_000721"
     CDS             16033..16503
                     /gene="comGD"
                     /locus_tag="ACG8M5_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0358000.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16475..16822
                     /locus_tag="ACG8M5_000722"
     CDS             16475..16822
                     /locus_tag="ACG8M5_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358001.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16785..17210
                     /gene="comGF"
                     /locus_tag="ACG8M5_000723"
     CDS             16785..17210
                     /gene="comGF"
                     /locus_tag="ACG8M5_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0358002.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17182..17541
                     /gene="comGG"
                     /locus_tag="ACG8M5_000724"
     CDS             17182..17541
                     /gene="comGG"
                     /locus_tag="ACG8M5_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0358003.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17713..18720
                     /locus_tag="ACG8M5_000725"
     CDS             17713..18720
                     /locus_tag="ACG8M5_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358004.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18745..19929
                     /locus_tag="ACG8M5_000726"
     CDS             18745..19929
                     /locus_tag="ACG8M5_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0358005.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20366..21949)
                     /gene="abc-f"
                     /locus_tag="ACG8M5_000727"
     CDS             complement(20366..21949)
                     /gene="abc-f"
                     /locus_tag="ACG8M5_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0358006.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22054..22182)
                     /locus_tag="ACG8M5_000728"
     CDS             complement(22054..22182)
                     /locus_tag="ACG8M5_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358007.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22527..22670)
                     /locus_tag="ACG8M5_000729"
     CDS             complement(22527..22670)
                     /locus_tag="ACG8M5_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0358008.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22893..23372)
                     /locus_tag="ACG8M5_000730"
     CDS             complement(22893..23372)
                     /locus_tag="ACG8M5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0358009.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23445..23729)
                     /locus_tag="ACG8M5_000731"
     CDS             complement(23445..23729)
                     /locus_tag="ACG8M5_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358010.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23807..24001)
                     /gene="ssrS"
                     /locus_tag="ACG8M5_000732"
     ncRNA           complement(23807..24001)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8M5_000732"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24073..25359)
                     /locus_tag="ACG8M5_000733"
     CDS             complement(24073..25359)
                     /locus_tag="ACG8M5_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0358011.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25497..26168
                     /locus_tag="ACG8M5_000734"
     CDS             25497..26168
                     /locus_tag="ACG8M5_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0358012.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26134..26622
                     /locus_tag="ACG8M5_000735"
     CDS             26134..26622
                     /locus_tag="ACG8M5_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0358013.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26635..27582
                     /gene="prmA"
                     /locus_tag="ACG8M5_000736"
     CDS             26635..27582
                     /gene="prmA"
                     /locus_tag="ACG8M5_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0358014.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27583..28347
                     /locus_tag="ACG8M5_000737"
     CDS             27583..28347
                     /locus_tag="ACG8M5_000737"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0358015.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28580..30793
                     /locus_tag="ACG8M5_000738"
     CDS             28580..30793
                     /locus_tag="ACG8M5_000738"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0358016.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30804..31250
                     /gene="dtd"
                     /locus_tag="ACG8M5_000739"
     CDS             30804..31250
                     /gene="dtd"
                     /locus_tag="ACG8M5_000739"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0358017.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31421..32680
                     /locus_tag="ACG8M5_000740"
     CDS             31421..32680
                     /locus_tag="ACG8M5_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0358018.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32769..33716)
                     /locus_tag="ACG8M5_000741"
     CDS             complement(32769..33716)
                     /locus_tag="ACG8M5_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0358019.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34110..35402
                     /gene="hisS"
                     /locus_tag="ACG8M5_000742"
     CDS             34110..35402
                     /gene="hisS"
                     /locus_tag="ACG8M5_000742"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0358020.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35417..37186
                     /gene="aspS"
                     /locus_tag="ACG8M5_000743"
     CDS             35417..37186
                     /gene="aspS"
                     /locus_tag="ACG8M5_000743"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0358021.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37295..38170
                     /locus_tag="ACG8M5_000744"
     CDS             37295..38170
                     /locus_tag="ACG8M5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0358022.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38436..40319
                     /locus_tag="ACG8M5_000745"
     CDS             38436..40319
                     /locus_tag="ACG8M5_000745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0358023.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40394..40678)
                     /locus_tag="ACG8M5_000746"
     CDS             complement(40394..40678)
                     /locus_tag="ACG8M5_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0358024.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40668..41402)
                     /locus_tag="ACG8M5_000747"
     CDS             complement(40668..41402)
                     /locus_tag="ACG8M5_000747"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0358025.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41531..42154
                     /locus_tag="ACG8M5_000748"
     CDS             41531..42154
                     /locus_tag="ACG8M5_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0358026.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42346..43467
                     /locus_tag="ACG8M5_000749"
     CDS             42346..43467
                     /locus_tag="ACG8M5_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0358027.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43474..46602
                     /locus_tag="ACG8M5_000750"
     CDS             43474..46602
                     /locus_tag="ACG8M5_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0358028.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46621..49815
                     /locus_tag="ACG8M5_000751"
     CDS             46621..49815
                     /locus_tag="ACG8M5_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0358029.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49961..50668)
                     /locus_tag="ACG8M5_000752"
     CDS             complement(49961..50668)
                     /locus_tag="ACG8M5_000752"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0358030.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16659 a         8920 c        10599 g        14748 t
ORIGIN      
        1 tagacttaca ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa
       61 ctaattggta tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa
      121 aatgcaagaa caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga
      181 gaagtagaag acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg
      241 ttaaatggtt ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa
      301 aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat
      361 ctcggaagtt atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta
      421 gggagtgaat tttctatcaa agagctaaac tatgagcttg agtatgaaaa atctatttgt
      481 tcaatccctt ataataatat tgaggaaatt atagaaaagg atgctatcag catttacaaa
      541 ttttttattt ttattcctaa tcgtcaattg aaagctaata tatttaacca tttgaaaaag
      601 atgctaaatg tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat
      661 gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg
      721 agaatcgcga tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt
      781 tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca
      841 aatgataaag acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa
      901 ggtagtatag aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat
      961 agtacttctt cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa
     1021 atcatgtcac aatctttttt tcgaataaac ttattttttg aagtcggcca tttatcgtaa
     1081 tttctttgca taatacgaac aaattttcgg ttattgatga aaaaaaacat tgaaaatggt
     1141 atgataaaag aaaaaaagga ggaaattttt aatggtgaaa tttatccatg cggcagattt
     1201 acatatggat cggtcatttg aaggactgac aactcttgat aaaactgtac aggaaaaact
     1261 attgaagacg aatctgaccg ttttatcaaa tatcattgaa caggcaataa taaataacgt
     1321 tgattttgtt ttacttgcag gagataattt tcatcaaaat cgtccttcat taaaaataca
     1381 gaaacatttt tcagaacaga tgaaacggtt agaaaagaac catatacctg tatttataat
     1441 attcggaaat catgatttct atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt
     1501 ccatttattt acatccgaaa cggtagagac aaaaaaaata acgataaaaa gtggagaaac
     1561 tgtctctctc agtggattta gttatcgtca accgtggatt caaaaagaca aagtgatgga
     1621 atttccttct agagaactca cagattacca tattgggttg taccacgggg aacctggcgt
     1681 ctctcaaaaa ggaaactatg ctcctttcca accctcaaag atgcaagaaa agggatacga
     1741 ttattgggct ttagggcata tccatgtgcc aacggtctta aatgaaaagc aaacaattgt
     1801 ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa gcttctacat ctattctact
     1861 agttgaatta agtaaaggaa gttgccaaat acatccgatt aaagtagcag aggtctattg
     1921 gaagacaaag gaaatatcat tgaggaaagc aagaacgaca aaagaggtca ttgttcacat
     1981 tcgtcagcag ctttcaaaag tagaggatgg atttagttta attgagataa aattaaaaga
     2041 ttatgaccat ctcaatacag atgttttgga acggatacaa tcaggagagc tattagacta
     2101 tttgttagag gaattctcag ataggataaa tgatttcttt atttggcgta tttctctcat
     2161 agagaatact ttttatacga agaccccact tgctgcttct gctaaattga tggaacagtt
     2221 atttcaatac tatcagacac ctcaagattt ccaacaaatt ttaaatgaag tatatagtca
     2281 tcaagaagcg gctagaatat tgagcgaact gccggaatat caagaagaaa cacttgaaaa
     2341 agccaagcag ctgcttaatc aagatttttt atttgaggag gatcaggaat gaagatttta
     2401 aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc
     2461 aatcagctga tctatggggg aaacgaagca ggaaaatcaa cgatttatca atttattcaa
     2521 gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac
     2581 ggctcagcct atggcggcaa aatatggctt aaacatcctg tatatggtga atttgctgtc
     2641 gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga
     2701 agtgatgagt tactagaaaa actgatcgca cctttgacga aagagttatt ccagcaggta
     2761 tttactttcc agcaagagca gttgatggaa ctagatcatt tgagagaaaa agagctacat
     2821 gatgcgctga tttctttagg catcacgggc agttctcaag tattccaaaa gcggcaagaa
     2881 tattatcaga aagcacaaca attattcaag aaaaaaggcc aaaaattact aatcaatcaa
     2941 aaattaaatg aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga acaggaagct
     3001 tattttggca agttgatcca acaagaacag gaatacgagt cgcaacagca gcagttattg
     3061 gcacaattaa aagaggcgaa caaacaattt tcattagcca gacagcaaga acttaatttt
     3121 tctttatatg aagaatggca atctctcaaa aaaaacagca ctacaggtct tcctgatgag
     3181 caacttcttt tagatctgga gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg
     3241 gaagaattag aagcaacact ggaaaagcat agcgggatgg atcagcagtc agccagatat
     3301 tatttttatt tagagcagga gcaggaacta aaagatctcc agcaacttca atttacagcg
     3361 gaacaattaa acgaagaatg gcaaagatta caaattgaga ctagagaaac aaattcagaa
     3421 ttgaagattt tggaacaaag gtggcattgg cagaaaaatc cgttaccgga agaattttta
     3481 tatgaagaag aatggaagca tcttctggct gaagaaagaa ggattgctga tctagaaagc
     3541 cagatttctg tgcgtctcca aatagctaaa gaacagcaag agatgattga agcagaagtg
     3601 aatgacttgg aagagaccta tccagcatta ttacaaaatc agtcagagat agcaccagtc
     3661 tcttcatcta aaccgtatat gctgatggct ggcggagcaa taatcattgc aggtatattt
     3721 gtaccaatgc ccttgaaagt gatcctttta ctaattggtg caggcatgat tggctatttc
     3781 ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt tctctgagat aaaaggaatg
     3841 tggcaagaaa aactaggaca acttgatcta gccttagctc aattagctga agcagaagaa
     3901 aaacaaaaga gaactggaga agaaaaacag attcttcagc aagaagtata tcgttttgcg
     3961 gatcatcggc acttaggaaa gatggatacg ctcaaactca tgaaagaata tggacaggaa
     4021 gtcatagatt atcagaaatt atatggcgta tttgagcgga aacataaaag agaacaggaa
     4081 atacaagaaa aactacgaca aatcgaccaa aaatttgagt ttgttaaaga atggttaccg
     4141 atacaagata agacaatttc agaaaaaatg gtattgcttt cccgttttat acaagagatg
     4201 gaacagatga aatttgccag aagctatcag caaaatactt tattgaagca agaaatcaat
     4261 caattgaaaa agaaacaaaa agtatggata caagaataca gcgaacaatt gactcaagca
     4321 ggcttatctt atccttcgga agtacctgtc tttttacaaa aagccaaaca agccgaacag
     4381 cagatgaaaa gatacaatga attatcggaa atgcttactc cactatttcc tgaagagctg
     4441 acaaaagaga aattatctgt aagactgcgc caactgaaag aaaagcaatc ccaactccag
     4501 caagaagaaa aagaagcaag tgaaaatcgg caacgactcc aacttcaagt agaagagttg
     4561 caaagagacg gcacattgga cgaactttat caacaagcta gccggcaaaa agcaaaatta
     4621 caggaattac tggaggaatg gggagcactt gaaatcgccg ggacattctt aggtgatctt
     4681 tcaacagaga tgagtgaaag acaattaccc cagttgttaa gagcagcagg tgattattta
     4741 ggtattttaa caaaaggaaa ttatcagaag atattactga aaaatgatac actgacttta
     4801 acagacggat atacagaatt tcccatctat gagttatcta caggtacgaa agatcagctg
     4861 attatggcga tgagatttgc ttacctctca ttagaagcta accgttcgat atgcccgatc
     4921 atcattgatg atggctggct tcattatgat catcagcgaa agtatcaatt agctgaactt
     4981 ttagctaaat ttggagaaaa gtatcaaata atttgttttt cttctgataa agaaatggta
     5041 agctattacc atgagatgaa ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa
     5101 aattcgtgat ttagtaatcg atgagcagtt tgaacaattt gtcctgatca aaagtgcaga
     5161 tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt actttccaag atacttcagg
     5221 tactatcgat ggtaaatact gggatgcttc ggaggaggaa atcaataaat ttacagccgg
     5281 cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat
     5341 tcttcatatg cgcttaacaa gggtagatga gccaggagag cctagtttat acatggaaag
     5401 agcccctttg aagaaagaag aaatggaaga agaaatcaat caattgttgt ttgagatcac
     5461 caatgcgcat tggaatcgta ttgttcgcta tctacttaat aaatatcaaa aacaattttt
     5521 tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc agtggattag cttatcatac
     5581 tttgacaatg cttcacttag ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc
     5641 attgttgtat gctggtatca ttttgcatga tttaggaaag gtgattgaac tttccggtcc
     5701 tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt catctggtat tgattgacga
     5761 agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag atgttctcgt
     5821 attacgtcat atggttttag cacaccatgg cttattagaa tatggctcac ctgtacgtcc
     5881 aagaataatg gaagcagaaa tattacatca aatcgacaat atcgatgctt cgatgcagat
     5941 gatgttgact tcgattcgtc aaacagaacc aggaaaatac accgatcgga ttttcggtat
     6001 ggacaaccga agtttctatg taccaaaaga tttatagcaa aaatttattt ttttcattca
     6061 aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt ttcttttatt ttgatgaatc
     6121 agttgtttct tcagttgaag attcttttgt tgaatcagtt gattttgtgt cagaactctt
     6181 tgttgaagct gtagtttcac ttgagtcttt cgtacttgat gaagttgttg tgaatgcact
     6241 caacacattt tcaaaggcat catctttgat ttttacattt gcatctttta attcttcacc
     6301 gattacttta gttgtgaatg cattgtcact tagtttagtt tcagtagcga tatcttttag
     6361 ttgatctttg tatttatcca tgtcattgcc tttattttgg tttttcacca ttttgacaac
     6421 gtagtattct gtcgcataag aagtaggatt tgttgtagta ataacatcgg agatctcacc
     6481 gtctttcaat ttgaaagctg cttcttttac ttctgcagga atagttgttg tcgttgagtc
     6541 gaatttaact ttaccgccat cttcttttgt ctctgtatca gttgatttgt ctttggctaa
     6601 tttagagaaa tcatctccat catccgctga ttttttcaca tcttttgctt catcttcact
     6661 tgataatttg atgatttgtg cttctacttc tggatggaaa gatttccaag cagttttcaa
     6721 atcgtcatct gtaatatcta cgtgagcttt caagccagct tcaaaagcta aattgttacg
     6781 aatatattct ttataagtat ctttcgtata tccagcagct tctaattgtg attcaaaagt
     6841 atcacctaat gatttcgctt gtttgtcgta ttctgcatcc acttgcttat cggttacttt
     6901 atcgccatat ttgttattga atactttata aatgatcatc cgttgtacta aggattggtt
     6961 cgatgattca agttttgctt catcatagaa atcagataca gtgatcgttc cgcctttcat
     7021 cgttgcgata tctttattag tatcgcctga acaagctgct agagttaaaa cagccaacgc
     7081 actagtagct gccaaaataa ttgatttttt tttcattaaa atttgcactc ctgtaattag
     7141 ttattatttt tatatattaa ctataccata tcccatttcc tttaaaaacg ttgtcgtggt
     7201 agtttcaaac aaaattcaaa atttcatcac attcctagga aatacgcaaa cttttctcac
     7261 ttatatagtt aaatctgttg agaaaataat cattattata tttgttcagg atactctggc
     7321 agatcagctt ttagtttttc tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg
     7381 aatttaaaat cttcgatatc ttttttgatt gcttcttgga aagcaggaat cacagattca
     7441 gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt atttaaatca
     7501 tttgtcagag ttgttgcttc ttccaattct tcgatcagtt tctttcttgt ttcattcccg
     7561 ctcctgggcg ccaataacag acctccgaca gtgccagcag cagctccaaa taacagtcct
     7621 ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg attgtttctt gaatagcttt
     7681 tagttcttcc gatccataac tatcttgatg attgacaaag tgaatagaaa aatcatcctc
     7741 tttgctgtag cgagggacta aatggatatg tgaatgaaaa acagattgat aggctagttc
     7801 acggttgtta ttcaagatat taagaccttt catttctgga aaagcttttt ccaatgcttg
     7861 agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat
     7921 atctgctaca tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc
     7981 taaaaaagca tagaccttgt cgtcttcata tattttatag cttgggattt cttgattgat
     8041 gattttgcaa aaaatacagt tttccatata aaacatcctt tctataaaga ataaatactt
     8101 cagcttaact ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt
     8161 tcttcgtggt ataataaata aaaataaatg gcggaggaaa aagaatgagt ttaattatcg
     8221 atcatgtcac aggggggtac ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac
     8281 agcctggaga gatagtaggg ttgattggac tgaacggagc agggaaaagt acgaccatca
     8341 agcacattat cggtctgctg gaagcttcaa aaggaacgat agaaatcaat catctatcct
     8401 taaagaaaaa tacggaagaa tatcgcaaac aaattgggta tattccagag actccttctt
     8461 tatatgaaga attgacattg agagaacata tcgaagtcac tgcgatggcc tacgatatac
     8521 ctatggatat agcaatgaaa agagcagaaa agttgttgga gacttttcgg ttatctaatc
     8581 gtcttgactg gttcccagct aatttttcaa agggaatgaa acagaaagtg atgatcttat
     8641 gcgccttttt gatcgagcct tctttatata tcatcgacga accattttta ggcttggatc
     8701 cactagctat caatgcattg cttgaattga tggtagaaat gaaaaaagaa ggagccggta
     8761 ttttgatgtc tacgcatatt ttagctactg cagaaaaata ttgtgatcgt tttgtggtct
     8821 tacataacgg agagatacga gcagacggtt ctcttgagat gttgagaaaa gaatttcagc
     8881 tgccggattc ttctttggac gaaatctata ttgctttaac aaaggagcag gcacatgagt
     8941 agtttttttc aaaaacgttt agcaagacat caaaagaaaa tgatgaaata tatgcgttat
     9001 gtattgaacg atcattttgt tttagtttgt cttttcttga ttggcggtgt gggattatat
     9061 tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct cataatctct
     9121 gtattttgga tgctctgttt attcgtgggt aagatggcta cttttgcaga ggcagcagat
     9181 attgtttttt tacttcctaa agaaaaagaa atgcaaacct atttggaacg agcttttcgc
     9241 tattcttgca tttttccgtt gatctttttg atattggcat gcggattggc catgccatta
     9301 gtggtggtta gtacggagca atccttttcg atgtttttcg tctatctgat catattatgg
     9361 agtttgaaat tcagtcatct gcaaatcaag agagcagaat tgtttcgact agatcaacaa
     9421 gttattcgtt attggaaatt aggctggttt ttttcttctc tgatcttttt gtttctatca
     9481 ttttattggc taatatgggc aggaatagca ggtgcattag tacaggcaag tctatattac
     9541 tggtttttgt ggaagcaaca agaaaaagga ttggattggg agaaaatggt gcatgtagaa
     9601 gaacataggt tgcatcagat ctatcaattt atcaatttat tcacagatgt tcctgaaatt
     9661 acggcaaaga tcaaaagaag acgctatttg gatcctatat tgactcatat taaaagagaa
     9721 tcaaaaaaca cttatcttta cttgtttgca agaagattgg caagaggttc tgattttagt
     9781 ggtctttatt tccgattagt aattataggt gggttattga ttgccggagt agtcgacttt
     9841 tattttagtc tgattatcgg tttcttattt ttatatttga tcggtttcca actgttacct
     9901 ttagcgaacc agttccgcta tatggttctc gttcagcttt atccaatcca taagaatcag
     9961 aagaagcagg cgattcagta tctactcaca tgggtattaa ttatcgcgtc ggttattttt
    10021 ggtttgattg ctgcagtcgt attagatggg cagcagcgat gggttccgct agcggcttat
    10081 ttgattgtta ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca
    10141 gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa
    10201 gaatgctgtt cgttctattt tttttgagaa aagctggttt tttgaatggc aattaaatga
    10261 aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga ctagaataat aagtactgca
    10321 aaacaaagag taagaggacg ggagaatgca tgatggagtt tcagttagac caagagtggc
    10381 ggttacgtcc catcaaaggc gacacaggaa aaacatacat aggcatgcgt gatgaggaca
    10441 aagtctttat taaacgaaac acgactccga tgttggctgc tctttcaagg gaaggaatta
    10501 caccaaaact tgtttggaca aaacgaacgg ggaatggcga tactttgaca gcacaagagt
    10561 ggctggaagg tcgcttgctg actcctaatg agattggtca aagaaacgac gtgattgatg
    10621 ttttgtacca tttacatcat tcaaattctc ttaaaaccat gctgacccgt atcggtggtc
    10681 gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa agaattaccg gaagaactcc
    10741 agaaaaatca atttctgcaa attgtccatc gttttcttcg tgagcagatc ccgtcaatag
    10801 ataaggaaaa cttatcagta gtacacggag atgtaaacca tcgtaattgg ttagtatgta
    10861 aaaattattt atatctagtc gattgggatt caatcatgtt tgcagaccct gcggtggata
    10921 ttgcaacgat tttaggaaat tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt
    10981 atggcatacg cccgactgac gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt
    11041 tgctccaaga aatcaccaga tattgcaaac gtgaagatca tcgacggatg aacgaagaaa
    11101 ttttacaatt aaaacgaatt ttcagtggct gaggtttggc ccaaactctg cggagatttg
    11161 gccagacctt tttaattaga ggaaaggaat ggagataaat aatgcgtgtc cgaaatcgtc
    11221 cgggagcagc ggaaatgtta gcggctcacc cgaattttgt aattagtgat ccaacattgt
    11281 ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc aatccacatt gagattggta
    11341 tggggaaagg ccagttcatc actggaatgg caaaagccca tcctgaaatc aattatattg
    11401 gagtagaaat gcaggtcagt gttgtttcga ttgcactaga caagttgatc gaacaaccgc
    11461 ttcctaacct gaaacttcta catgtggatg gatcagcgtt gacagagtat ttcgctgaca
    11521 gcgaagtaga tcaaatctat ctgaattttt ccgatccatg gccaaaaaaa cgtcatgaaa
    11581 aacggcgatt gacatataag acttttcttg ctgtagatga acagattctt cgtccaaatg
    11641 gagaaatcca tttcaaaaca gataatcaag gattgtttga gtattctctg gcaagttttt
    11701 ctcaatatgg catgatcctc aaacaggtat ggttagattt acatcaaagt caatttgaag
    11761 gcaatattat gacggagtat gaagaaaaat tttcttcaaa aggtcaacgt atttatcgag
    11821 tagaagctca atttcaagat aagaccaaaa agaaaagtct gtgaaactaa ctttctgctt
    11881 tcataaaaac attaatatcc ataaataaat cccagaaaat gaacattaca gatgtaatgt
    11941 attttctggg atttatttga tttaaaatgt atttatttca tcggtttttc cacaaggtgg
    12001 aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg gtgatcttca attcgggtat
    12061 gacagataat gttaagaaag aaagtgtcaa aaaaggatca aaggttttag ctgctcctag
    12121 ttgatgtgct ttagttatta attcattcaa ctgttcatag acgatcgaaa aaggttcctg
    12181 tgtcatgatt ccgcctatag gaagagaaac acaagctatt tcctgctgtt tgttgacaac
    12241 ggtcatcccg ccaccttttt gaatcaaaag attagcagca tacagcatat cctcgtcgtt
    12301 tgtcccaacg acaactagat tatgcgagtc atgggcaatc gttgtcgcta tcgctccttg
    12361 tttcagctga aatcctttca ctatgcctaa tccaatgttt cccgtttgat ggtgacgctc
    12421 gatcaccgca atctttaatt ggtctttttc gattgaagga atgaatcgtt cgccttgtcg
    12481 gtccactttt tctaccaaat ctttagtcac tagactattc gggataattt cgatcacatg
    12541 ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga tttaatggtt gagcgtttag
    12601 ttttggcaga caacacgtat atgaatttga atgatcagca aataaatggt cttggagttg
    12661 cccagattga acaactaatg ttcccttata gaaaacttca tgaattggca gcttggtaag
    12721 atcgtctgtt aagaagaaat cagcttgata cccaggggca atcgcaccta gtgttttcaa
    12781 atcaaaacat tctgctgcat tcaatgttgc catctgaatg gcttgtaatg gatcgattcc
    12841 aagtgcaata gcttgacgga cgatatgatc gattgatcct tcttttacta aatcattgat
    12901 cagtttatca tcggtgacaa agaagcatct tcttgaattt tcaggtgtga tcgccggtag
    12961 tagcgatgcc agatcttttg caacggtccc ttctcgaacc attaaataca taccaagatc
    13021 caaacgttct ttagcttctt ttgcagttgt agcttcatga tccgtccgaa tacctgctgc
    13081 aggataaaca tttaactctt ccatgccgat accagctgca tgcccgtcaa tttttttctt
    13141 atgctggtgc atcaattgga tcttttctag gatgtctgtt tcattatttg ctactgcttg
    13201 atagttcatg acttcagcta agcctaaaac ttcgggtttt tgtaaaaaag gggctaattt
    13261 ttttgcattt aaatttgcac cattggtctc aaaaggggtt acaggtacac aagaaggtag
    13321 cataaaaaag atattcatga gtgtttgtct tgcatcttct atcataaact caagacctgt
    13381 actcccagca atgtttccta tttcatgggg atctgtcact acagtagtaa ctccatgaag
    13441 caaggatact tttgaaaatt cgcttggtga taaaagtgag ctttcgatat gcacatggcc
    13501 atcgatgaat cctggcacta gatattgccc attagcatta tgtacgcttt ttccttcgta
    13561 ttcaccgatt cctacgatca ttccttcgca gattgctaca tctttttcca tgatttcttt
    13621 cgtaaagacg ttgacgatct tagcatgctt gatcactagg tcagcaggct tttttttact
    13681 tgctacttcg atatatttcc ttaatgttgt ttccaatgga gtgcctccca agataaattt
    13741 ttataagtat aacataaatt cggtttttgc caatattttt ttgtaaattc attttatttg
    13801 aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg gagaataatg gaaataaaac
    13861 gattatcgga tcaaatgata agtgaaagtt ttgccaaaca tgcccaagac gtatatatat
    13921 tgccagcttc agaagtattt tacatctatt ttcgctatgg aaaaaaacga acattctgga
    13981 agaaagtctc ttcggcagat ggacagcaac tgatctcaag atttaagtat ttgggacaga
    14041 tggatatcgg agaaaaaaga aaagctcagt taggtgcatt tacttattct ttagaaaaac
    14101 aggaagttcg tctgcggttg tcttctgtag gagattacct tcaacgagag agtttagtga
    14161 ttcggctatt atacggtact tttgaaacac cccatcgttg ctttagttct cgagattttg
    14221 caactatttt acagaaaacc gtcagacgtg gattatacct tttcagcgga ccagtcggat
    14281 caggcaagac ttctctaatg tatcggttgg ctttggaaga aaaattgcaa gtgattacta
    14341 tcgaagatcc tgtagagata gaagaaccgt tttttctgca gctacagatc aatccgaaaa
    14401 tagcacagac ttatgataca cttttgagac tcgcattacg gcatcgtcca gacttgctaa
    14461 tcattggcga gataagagat gaatcaactg cacatgctgc ggtaagagca gcactaacag
    14521 ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga aggtacattg gtcagaatcc
    14581 atgaactgac aaagaaaaaa gaagagctat ctgaatgttt agctggtgtc atttatcaag
    14641 aacttttgga agatgccaat caccatcctt ctgttttatg gagttatcaa tttatggagg
    14701 aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga agctcaggaa aatatttggc
    14761 agtcagaact attctaaagg attaacaaga agccagcaaa accagtttgc acaaaccatg
    14821 ggagaactgc ttttatctgg ttttagtctg caagaatccc tttatttatt actcacgatc
    14881 cgttcgtttc cacctgttat tttggcaaat atccaacatc agctaaagga agggcaagcg
    14941 tttagtgagg tactttcgca aataggatat ggagcagagc aactggtaca gatcgagtta
    15001 gctgaacgtc atgggaatct gccgacaact ttgttggaaa ttgccaaaca gtttcggctt
    15061 gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat atccgatgct attgctcacc
    15121 tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt tgcctcagct tattggttca
    15181 ggaatggtca tgcaagagca ttggggtatt cagctgatac aagtctttca ttggtatgtt
    15241 ctactattca tcctagcggt tactctattg gctttactag taaggtatcg tctgatgaag
    15301 ttgtctcctg ttgatcagag tgaatggata agcagacaat tattgattgg accgatatat
    15361 tccttgtatc aatcggctta tttgtcacta gagattggaa agctttttca tgaaggtttc
    15421 gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac aagaaagtct gattcagcat
    15481 cttgcctcta agttgattca aggattggag gcaggtatgt cattggctga gcagttccag
    15541 aattatcgtt ttctatcaga agaattcagt aaaattgttt tacagggaga ggcaaaggga
    15601 agtttaggta aagaactgat tttttacagc gaatggacaa gaaaacagct tttcaaacgt
    15661 cttcatcact ggatgcattt tattcagcca atcatttttt tgggcgtagc ggctttgatt
    15721 ttattagttt atgccgctgt tttattacct gtttacggaa gcatagagga ggttttgcca
    15781 tgaaaaagag aaaaatatct tattatagcg gttttacatt gatcgaaatg ctaatcgtct
    15841 tactgatcat cagtgtatta gtgttgttgt tcgtccctaa tttgtcgcgt tataggaatc
    15901 atgtagacca agaaagtcgt gaagcgatca tccagttggt agacacacaa aaagagctct
    15961 atgcgcttca aaataatgga agagttccta ctgtggagga attgttgaat gaaggctata
    16021 tcaaacgaga acatgcagaa atctatcagc ggccttgaag gtaaggtttt agcatcagga
    16081 tatacgttgt ttgaatcact cttactgatt ttattactta caacgatcct ttcttttcca
    16141 gttctcgcat tttctgcatg gaagaatgaa ttggcagtct atcaattttt cggccaattt
    16201 gaaaaacgaa tctattctac gcaaaaaatc gctattgtga atcagatcca aacaggcttt
    16261 tattggaata gtgaagagaa tcaaatcatt tttcatgttc cgaatccaga taaaataaat
    16321 tgggaagtat tagatattcc tgatgagatt accttgaaac gccatgcttc tattactttt
    16381 gctgctagaa ccgggaatga aagttcacta aaagcttatc aattttattg ggaagaaaaa
    16441 gagcggacga ttacgtatca attccagata gggagtggtc gctatacaaa aagaattgaa
    16501 taaaacagga tttgttttgt tggagtccct tacagcgctt atgatcgctt ctgtctgtgt
    16561 ctacttagtg agctttggcc agctgttttt gcttcaggca gaaaagaaaa gtggggagga
    16621 actgctattt attcgaatga tttatgaaga tatcaagacg cagcggatgt atcacaaaag
    16681 accagagcga agggaagaaa tgatcccata cgatatccaa attgtcgata ataatggagt
    16741 aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc agtattgaaa aaacaagtaa
    16801 ttccagcatt tacgctgatt gaatgtctaa ttggactgat gatattgagc agtctattgc
    16861 tcacattcaa tcttttgatt cagcaaacca tccaagtaag tgaatttttg cagcgtaaag
    16921 atcaaaaaga gtggctaata tttttagcac aattagaaga agaactcaag agcagtcaca
    16981 atgtctctgt gaaaaatcag caactgcact acacgatcga ggataaacag tatatcatcg
    17041 aaagatatca ggcaatgata cgtaaaagaa gaacttccgg cggacatcaa cctatgttaa
    17101 caagtatcac tgagcttcaa ttattggaaa aagaacacac aatttctttt tatgtacact
    17161 ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa cgatcagtag aaaatgttat
    17221 aagggctcta ttttattttc tgtcttgctt ttatttcttt tatgtacgtt tttattgatg
    17281 gcagcattag aaagctaccg tttgtcaatg gatcttaata tacgaatcaa aaattattat
    17341 atagcaaaaa taatgaagca gatggcttta tcggaacagt ctacgcttgc cgaaagttcg
    17401 gaaggagttt tttactacac aactggatct gtaacatatc agtgggtaca acagtcccta
    17461 ttcttagagg tggaagtctc accgtttatc tttcggttca cagaagaagt aaaaaatgat
    17521 acagatacta gtacagaatg atggaatgtt cgtgaaaacg ccctcttttc tgttataaca
    17581 ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa aaacagtgaa aacgattgaa
    17641 attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa ttgttataat atatctagaa
    17701 atgaggtgca ccatgttacc agaaaaaatg gaacaagggt ttaatcaaaa cctggaagct
    17761 attcagcttt tacagaatgc attgggaact tcctttttag aagcctatgt cgaaaatgca
    17821 gaaaatttaa tagatgatta tcaagtacgt gtggtggatg gagtgccgac aaaagagaca
    17881 acgcaacgga tcacagctct ttatgaagag ttgaagaaac tttctttcga gccagaagaa
    17941 tggcgcagac tgtctcaatt acttttattg aagggcagcc agacagaaca tttgcaggcg
    18001 aaccatcaac tcactcctga tagtatcggt tttttatttg tttttcttat tgaacaacta
    18061 tacacaaata aaaaagcacc ggttaagatt ctggatattg cggcaggaat gggcaacttg
    18121 ttattaacag ttcttttaaa cctatcgaat gcggggtacc aaacagaagg aattggtgtg
    18181 gatattgatg atacattgtt ggctgtcgca gcttctacaa gtgatttgac acaagcaaat
    18241 gttcaatact ttcatcaaga cgggttgcag gaattattaa tagatcctgt cgattttgca
    18301 atcagcgatt tgccaatcgg ttattatccg aatgacgaaa aagcaaaaga gtttttaaca
    18361 agtaccgaag aaggccatag ttatgcacat catttattgc tagagcaatc aatgaaatat
    18421 gtgaagccgg atggttttgg tttgtttttg atgccgagtg gatttttaga aacggatcaa
    18481 agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc ttcaaggaat gattcaattg
    18541 ccagatgaat tgttccgtaa taaacaatcg caaaaaagta ttttgatctt acagaaaaaa
    18601 ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga aacttgcttc attaaaagaa
    18661 ccagagaagg tgacggaatt tttcaacgag tttaaaaact ggaaatcatc tgctctgtaa
    18721 tcattcaacg agaaggagag tcatatgtct aaaacaattg caatcaatgc tgggagttca
    18781 agtttgaaat ggcaattata tcaaatgcca gaagaaaccg tagttgcgaa agggatcgtc
    18841 gaacgtatcg gtttacaaga ttctattttt accatcaagt acggcgataa tcaaaaattc
    18901 gaacaaatca tggatatcga aaaccacgaa gtagcagtaa aaatgctgtt agatcagttg
    18961 attgaactgc aaattttaag ctcttatgat gaaatcaccg gagtagggca ccgtgttgtt
    19021 cacggaggag aaacttatgg ggattcagta gtgatcgatg agaaagtaat ggaccgcatt
    19081 gctgaattag cagaatttgc tcctttgcat aatcctgcaa acttaatggg gatcaaagcg
    19141 tttaaaaaga ttttacctga cattttgagt gtggctgtat tcgatacatc tttccactca
    19201 acaatgccaa agcaaaatta tttatatagc ttaccggtgt cttattatga agatttcaaa
    19261 gtaagaaaat atggcttcca tggcacaagt catcgttatg tgtcggaacg tgcggcaaaa
    19321 ttattgaaca aaccgatcga agaactaaaa atcatcactt gccacttagg aaatggtgta
    19381 tcgatcacag cagtcgatgg aggtaaatcc atggatactt ctatgggatt cacaccatta
    19441 gctggggtga caatgggtac acgttcaggt gatatcgatc cagctgttct cccatattta
    19501 atggaaaaat tagagattga tattgatgaa atgatcaatg ttttgaacaa gaaatctggt
    19561 ctgctaggcc taacaggatt gtctagtgat atgcgtgatc ttgaaaagaa ctatgaaaaa
    19621 gaacatattc gtttagctta tgacattttc gtggaccgca tccgaaaata catcggcggt
    19681 tatgtgacaa cgatgaatgg tgttgatgca attgtcttta ctgctggtat cggcgagaat
    19741 gacggacatg tacgtagtga aatcatcaaa ggtatgtctt ggtttggttg tgaaatcgat
    19801 ccagcgttga atgaattgcg tggtgaagaa gtagatatct ctacaaaaga atcaaaagta
    19861 agagtattag tgatcccaac agatgaggaa ctaatgattg cccgtgatgt ggagcgtctg
    19921 cgcggctaag ccaaagcatt cgttaggata aaagatgaaa cgcatgcaag agggaattct
    19981 tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa tttaacttca atttcccttc
    20041 ttttttacag gatcttctta gggaattttg cttggaaaag tggtcaaaag ggaaaagact
    20101 ggcattttat ttattgattt tcaggaaaat cccaatattt tattttcatt catctttata
    20161 agggacatta catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac
    20221 tttattcttc gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa
    20281 taaaaacttg attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc
    20341 aagttttttg ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga
    20401 tctgctcctg atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat
    20461 ggctgactgc taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag
    20521 catagctgtc gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa
    20581 agagtttcag caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac
    20641 ccacatcgct ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc
    20701 cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct
    20761 gcggcaaata accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt
    20821 cctcaccgag taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg
    20881 ctaaatgatc tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt
    20941 actgctggtg caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca
    21001 agctttgcag ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc
    21061 ggcgttccag ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact
    21121 gacgaaaacg acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat
    21181 tttttgtcag ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg
    21241 gatactcaat caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa
    21301 attgccgatc atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca
    21361 accagcgaat actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg
    21421 catcggctgc taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg
    21481 ttaaccgtgc ttcctgacgg tgtggaatcc ctaagccttg caagatcgct gcaatccggt
    21541 cttctaatac gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca
    21601 gacacttctc taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct
    21661 gtttgaggtc ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg
    21721 aaactgtaag ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac
    21781 cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt
    21841 tgataccgac taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata
    21901 atggattccc gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc
    21961 ttgagaccac tcaaaaaggc catggttatc catggcctgt gttatttaaa ctcagattat
    22021 catgagaaaa ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa
    22081 acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg
    22141 ttggtaattt tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg
    22201 tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt
    22261 acttaatatt tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa
    22321 tgtctcgtgg accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg
    22381 gtgtttgata aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc
    22441 attaaacagc tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg
    22501 ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt
    22561 gccttctcgc ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc
    22621 gccatcgata cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac
    22681 caataagctt tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg
    22741 tgttcacttt tttttatttt acttgaactc attgactgtt ttccctttat tttaaacatt
    22801 ataaagaccg atgagtgctt gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa
    22861 aacctgccga tagttggcag gtcgtctacg tgttaagctt tggtgtggtc tttagctggc
    22921 aattggtttt ctaaatcagt tcggacaaca agcacatcgc aagctgcgtt tctaataaca
    22981 tattcagaaa cagaaccaat aaataaacgt tctacagcat tcaatcctgt ggctcctaac
    23041 ataatcaaat ctacttgatt atcttctggg atttctctgg caataatttg ttttggtgag
    23101 ccatattcta cgacagaggt tacattattc aagccagctt tcttcgcatt gctttcataa
    23161 tctgccaatg tttgctttgc catttcagta gcttgttctg caagcattcc atcaaaggat
    23221 gaaacggttt ggaaagaacg tgtgtcaatg acatgtgcta atagaagttc tccgttattt
    23281 cggatagcta cattgaccgc ctttttaaaa gctaattcag cttcatcaga accatctacg
    23341 gcaaccatga tttttttgta ttgttgtaac ataagcattc actcctcaag tcaatcgtat
    23401 tgtactcttt tattgtagaa cactttaggg aaaaagagaa ttatttactg ctttggttca
    23461 gcaatttttt ttgcaaatcg gatactgaag gtggcagaaa caataattac gatcagtaaa
    23521 aagaccaagg caataaagcg ttgattgaaa aaagctgtta aaatacccat tacgcctaaa
    23581 acagcatatg cagctccgta aaattgtagg aatccttgat ttttatcatt tttgttgatc
    23641 aatacaaaaa agatatcctg tttattaaat aaataatagc taacgaataa taatactaaa
    23701 gtggcgataa tcattaaaat cctaatcatg aacaaatcct ccaaaataag caacaaatag
    23761 aaaaagcaat ttgccaagag caaattgctt tattctttaa aagatggtaa tctccgttaa
    23821 tgccgttgtt tgtcctgtag tattgatccc gcgaattcaa gcaggtgggt cccatggtcg
    23881 tagcttcgaa ctaccaaatg ataaggcatg ttaccagcag tgacacaaga cgggtcccaa
    23941 gatatcaata aaaatgttcg gtcaacacac aaagaggaca attcgaacat cacagatttc
    24001 ccacctctat agtagcatat gaatttaaaa agagcaatga gagagctcgc agaaacagaa
    24061 ccgttttctt gtctagtttt ttggaggatg ctttttccac tcttttatac ggtaatactg
    24121 ttgacccaga gcctgttcat atttccctgt atcttttggc tgataatact gagcattctt
    24181 cagtttatct ggtaaatatt gctgattgac ccaagcctga tcaaaatgat gaggatactg
    24241 ataacccacg ccacggttga gtgatttcgc acctttgtaa tggctatctc tcaaatgatc
    24301 aggaacatct ccagctttgc cctctcgaat atcagcaagt gctgcatcta atgccatgta
    24361 ggcagaattg gattttggcg acaagcaaag atcgacgacg acgtctgcta gtgggatacg
    24421 tgcctcaggc aatcccaatt tttcagcagc taatacagcg ttcactgtac gagccgcagc
    24481 agcaggatta cctaaaccta tatcttcata accgataacc atcagacgcc ggcaaatact
    24541 tgccagatca cctgcctcaa ctaaacgggc gagataatga agtgcagcat ccacatcact
    24601 tccacgaatc gatttttgaa aagccgaaat aacgtcataa tgagcatctc catttttgtc
    24661 atgggtaaga gcttttcttt ggacacattc ttcaatgatc gacagtgtaa gatgaatcct
    24721 tccttcttta tctgaaggag tcgatagtgt agccagttcc agcccattca atgcactccg
    24781 caaatcgccg ttcgttgcac gcgaaaggtg aagaagagca tcttcgtcta actggattgc
    24841 ttgttgtcca aggccgcgtt ctttatccct taaagcatgt tcgactgcca actggatgtc
    24901 ttgttctgtt aatggtttga cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat
    24961 ggtaatataa ggattctcag tagtcgctcc gatcaagatg attcggccac tttctaaatg
    25021 aggaagcaga aaatcctgtt ttgttttatc taatcggtgt acttcatcca ataagaggat
    25081 cactgtacca ctcatttttg cttcttccgc tacaacttgt agatcctttt ttgaatctgt
    25141 agcggcgttt agcatgcgaa atgcataatt agtagagccg gcgatcgcac ttgcgatact
    25201 cgtttttcca gtaccaggag gtccatatag aatcatggaa gagagcatgc gagcatcgac
    25261 catgcggcgt atgatttttc cttccccgac tagatgctgc tgaccaacga cttcatcgat
    25321 cgttcgaggt ctcatgcgat aagccaatgg ctgttgcata ggattttcct ccttcttgtt
    25381 tttacgtccc ttaattatat catatttagc aagatgccta gaaagaacag ccttctgttg
    25441 ttttgttttc atagcttatc caactaatat tgtgtatgat aaagtaggtg agaaaaatga
    25501 cggataaaca aaagatagat gaattttttt ggaataaaac gacgccagaa gtggcacggg
    25561 atttattggg gatgtatttg gagcatgaca caccagaagg aagattagct ggatatattg
    25621 tagacgcgga agcataccta ggtccagaag acgaggccgc ccatagctat ggtttaagaa
    25681 gaacaccgcg tgtgcgggca atgtatgaaa aaccaggtac gatctacctt tacacgatgc
    25741 atactcatcg gattttgaac atcattactc agccagaagg gattcctcaa ggagtaatga
    25801 tacgtgcaat cgaacccgcc gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa
    25861 ctggtccaga tatcagcaac ggtccgggaa aattagtgga agcattagca attcctcaag
    25921 aactttatgg tcagtctata gcagatagtt cgttacgctt agtttttgag aaaaaaaaga
    25981 caccaaaaaa gatcaatgct ttgcctagaa tcggtatccc aaacaaaggt gtatggacag
    26041 aaaagccgtt gcgttttgtt gtgtcgggaa atccttatat ctctttacaa agaaaaaacc
    26101 aaatcgagaa agattggggt tggaggaaag aaaatgaaaa agaaggatct attaacatat
    26161 ttagatgaaa taattgaaaa acaagtgaca gactatgatg tagctttaga ttgggataca
    26221 aaaaatcata cgatcgagat cgttgttcgc ttgtttgcag aaaataaagc acaatttgag
    26281 attgatgatg cagaagggat cgtttcgcaa gaaccaatta tcgaatttga agacggcgta
    26341 cttcttttta atcctcagaa atctgttttt gatgaacgca attatctagc agtaatccct
    26401 tatgaaggaa aaaaagggtt ggcaaaatca gtagctgatt ctttagtgga atatctaaat
    26461 gaagtattag ctcaagggca aagcgatttg cttgattttt tggatgaaga cgatgacgaa
    26521 gctgtattcg agcttcactg ggataatcag caactagcag aattagtaga agtaaaacag
    26581 caaaatgaag cagatactta tttaccgtac ccaagttatt aaggagtgac agatatgaaa
    26641 tggaatgaag taaaagtaga aactgaaagt gaagcagtcg aagcagttgc taatattttg
    26701 atggaggcag gggcgagtgg tgtcgcgatt gaagattctt tagatgttga acattttcag
    26761 tcggatcaat atggtgagat tttagataaa aatgatttta catcgctgaa agaaggcgca
    26821 ctagtcatgg cttattttcc ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa
    26881 gatcgtatca cacaattacc tgagtttggt ctagctattg gaaaaaataa agtaacagtt
    26941 agtgaagtag aggaaagcaa ctgggcaaca gcctggaaga aatactatca tccggtgcgt
    27001 attacgcgaa tgctgacggt tgcgccaagc tgggaaaact accagacaga agatcctcta
    27061 gaaaaaatta tcacactaga tcctggtatg gcatttggca caggcactca tcccacgact
    27121 tccttgacat tgacagcact agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg
    27181 ggaactgggt cgggcgtgct aagtattgca gcaaaatatc ttggtgcaaa agatgtctat
    27241 gcctttgatt tagacgaagt agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa
    27301 atagccaaag atgtccatgt atcagctaac gatcttttga caggaatcga gattgaaagt
    27361 gatgtaatcg ttgcgaacat tttagcagat atcatcttat tgatgatccc agatgcttgg
    27421 cgtttactga agcaaacagg cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa
    27481 atggttctgg atgccatgtt agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa
    27541 gactggtatg caattatttt gaaaaaacca gaggtggaat agatgcagcg gtatttttta
    27601 aaagaatcat atgaatcaaa agactatttt gttttagaag gggaaaacta tcaccacatt
    27661 gtacgggtta tgaggatgga acccgaggat gaagtttttt tggcattcaa tgatcaaata
    27721 gccattgttg cagaaatcac ggagattact gaagaggctg tttatctgaa agaaattaaa
    27781 aaagaagaaa atcaaaagga actgccaagt tcgattacca tagcgagtgg ctatcctaaa
    27841 ggggaaaagc tggattggat cgttcagaaa tccactgaat taggagcgca agcttttctt
    27901 ggtttcccgg catcagcctc tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa
    27961 caacggcttg aaaagattgc tcaagaggca gcggaacaat cccatcggca gatcgtgcca
    28021 tcagtagagt tattggaaac gaaacaagat ttatttgagc ggctagatga ttatgacgtg
    28081 gtcttggttg cttatgagga atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt
    28141 cttagtgaaa caaaaccaga tagtcgaatc ttagctgtct ttggaccaga aggtggatta
    28201 tcccgcgatg aagtcgatta ttttgtagaa cacaaaggga tactttgtgg attaggacca
    28261 agaatccttc gtacagaaac agcacctctt tatttattaa gtgctgctag ctatcaatgg
    28321 gaattaatga atccttcttt cagctaacgt tcttcattaa taaaagagta tcgacagaaa
    28381 gaattgaaaa aatagtcaac aatttgtata atagaaacaa ttatttatac aatggatacg
    28441 aaaaatcgtt tgaaaaagct tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc
    28501 acggattttg ggacaattat caaacagata tttgtcccga ttcccgtttt accgaaaaaa
    28561 gaaaggggga tcgattcctg tgacaaaaga agaaatttta acgggacccg gtgtcataaa
    28621 gctcgttagt caatacatgg gtcccgaaca tgtagcattt gttgagaaag catgcgaata
    28681 cgcgactgct gcacatgacg gacagtttag aaaatcaggc gaaccctaca ttattcatcc
    28741 tatccaagtc gcaggtatat tagcagattt aaaaatggat ccccatacag tggctacagg
    28801 cttcttacat gatgttgttg aagatacaga aatcacttta gaagatctga gagaagaatt
    28861 tggcgatgac gttgctatgt tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa
    28921 atcccacgaa gagcagctag cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga
    28981 tttacgagtt atcatggtca aattagctga tagactacat aacatgcgta cgttgaagca
    29041 cctgcgagaa gataagcaaa gaaggatcgc tcaggaaact ttagaaatct atgcgcctct
    29101 tgcacatcgt ctagggatca gccggatcaa atgggaatta gaagatacag cacttcgtta
    29161 tctaaatcca aaacagtatt accgtatcgt ccacttgatg cagacgaaga gagaagaacg
    29221 tgaaaaatac gtaagtggta ctgttgaaga tattcgaata gcgacagaag agctggggat
    29281 ttttgcagaa atctatggac ggccaaaaca catttattca atctatcgta aaatgaaaga
    29341 tcagaaaaag cagttcaacg aaatttatga cttgctagct attcgagtga tcgtagattc
    29401 aatcaaggat tgttatgctg tattaggagc aatccacaca aaatggaaac caatgcccgg
    29461 cagatttaag gattatattg ccatgccaaa agctaatatg taccaatctt tgcatactac
    29521 cgtgatcggt ccggcaggaa atccggtaga aattcaaatc cgaacacagg aaatgcatga
    29581 aatcgctgaa ttcggggttg ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt
    29641 agaaccagat ggtatgacga aacaattaag ctggttccat gagatactcg aacttcaaga
    29701 cgaaagctat gatgcttctg aatttatgga aggcgtaaaa ggagatatct ttagtgataa
    29761 agtctacgtc ttcacaccaa aaggagacgt tactgagtta ccaaaaggat ccggaccatt
    29821 ggactttgca tacagtatcc atacagatat cggtaacaaa accactggtg caaaagtaaa
    29881 tggcaaaatg gtgcagcttg attacaaatt gaaaaacgga gatatcattg agatcatgac
    29941 ttctccaaat tcatttggcc caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc
    30001 aagaaataag atcaaacgtt tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg
    30061 ccacgaatcc gtggtcaaat gtattacaga tctaggattt acgcctaaag atattttgac
    30121 gaagaacaaa ctgcaagaag cactcgatcg ttttaattat caaacagaag atgatctcta
    30181 tgcagctgta gggtatggag aagttagccc cttgacgatg gccaatcgtc tgactgaaaa
    30241 agaacgtaaa gaacaaaaaa tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca
    30301 gccgaaaaaa gaacctgaca aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg
    30361 ggtagaaaac ttactaattc gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat
    30421 cgttggttat atcactaaag gcagagggat atccattcat cgtcgagatt gtccgaatgt
    30481 tcagcctgac aaaccaaatg tagcagaacg tttgattgaa gtcgaatggg aagatacatc
    30541 gaatacacga aaagagtatg atgcagattt ggaaatttac ggctataatc gttcaggctt
    30601 attgaatgat gtacttcaaa cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc
    30661 acggacgaat aaagataaaa tggcgacgat ccatttgacg gttggtatcc agaatttatc
    30721 ccacctaaag agtatcgtgg ataaaatcaa agcagtacct gatgtctaca gtgtacgccg
    30781 gacgaatgga taggaggaca gatatgagag cagttgttca acgggtaagt aaagctgctg
    30841 tgtcagtcga tcagcaagaa attggaaaaa ttggctacgg cctacttgtt ttattaggca
    30901 ttcatgaaac cgatacgcaa aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc
    30961 gtatttttga agatgaacaa ggaaaaatga atattagtgt agaagatgtg ggaggacaaa
    31021 ttttaagtat ctctcaattc acactgtttg cggatacgaa aaaaggaaat cgaccaagct
    31081 ttgtggaagc cgcacgacca gaaacagcta ttcctttata cgaatcattc aatgaaggaa
    31141 tcagaagtcg cggcatcaca gtcgctaccg gacaattcgg agcagatatg tcggtttctc
    31201 tcgtcaatga tggacctgta acgatcatta ttgattctaa aaacaaataa agtaatctgg
    31261 ttttcactag taaaaactat gaaacacgtc gcttaagtta gactgataaa ctctaactta
    31321 agtggctttt ttgtctgcaa aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc
    31381 ttaaattata caagttattt tttataggag gttgctgaag atgaaagaag taccagtagt
    31441 agatatcaaa atcacagatt taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga
    31501 gttacgccat gaagcggacg ctcccgtcgt caaagcaaag attttcaata aagaagctat
    31561 cacgatctac ggatcatcag cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa
    31621 aggtgctatg ccaaaattag tattgaagac actttttggt caaggtggag tacagacttt
    31681 ggatggagct gctcatcacc atcggaaaaa tatttttatg gatctaatga caccggaaag
    31741 aatggaagat tatcatcgta tcttagataa gaatctgaca caagcacttg aagcacagca
    31801 tggtcaattt gaactatttg atttaagtaa gatggttttc tttacatcta tttgcgagtg
    31861 ggcaggtatc aatttatcag cgatttctaa agatgaggtc gaaaaattag cagaatatca
    31921 aatctctatg atcagcggaa cgtttacttc tccgattgat cacataaaag gtgtcgaaaa
    31981 tcgcaaaaaa tcagaaaaat gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt
    32041 ggctggaaaa gaaaatgtgg cattatatgc ttttgccaat gccacagatt tagatggtca
    32101 gcttttacct ctggaagtgg cagctgttga attattgaac atcatccgtc caacggttgc
    32161 tctaactgtg tgggcggcat tgatgggaca tgcacttttc agtagaccag atttgtatca
    32221 gcaattaaaa aatgactttt cgacgttaca agatccgttt attcaagaaa tgagacgcta
    32281 ttatcctttc ttcccaatgc tacctgcgat ttcgctgaaa gaagtagaag tagatggtta
    32341 tcgtatacca gaaggcagtt gggttatttt agatttatat ggaacagacc atgatgaacg
    32401 aacagttgaa gcgccagatt cctttatgat caaacggtat gtagggaaag caaaagatat
    32461 ttcctataaa gaagaatatg agatgattgc acaaggcggg ggaaattttc gacaaatgca
    32521 tcgctgtgca ggagaatgga tcactcttca tagtctacgt gtgttttccg atcagctagt
    32581 aaataagttt gagttctctg tacctgaaca agattggacg attccattca atcagtttcc
    32641 gacttatccg aatagccgag cgctacttta caagaattag attagcataa acttatcgaa
    32701 aaaaaggaag atcaaacagt gctgatcttc cttttttcat attactataa attgataagg
    32761 tcaattgatc aaaatccacg aaatgcttca tcaatggttt gataatcttc attggataaa
    32821 tgcaaatcta aagcttggac attgtcagat acttgctctg gacgcttagc tccaggtata
    32881 actacagaca ctcgtggatt cttcatgtac caagctaaaa caagttgcgc aggagtggct
    32941 tggtatcttt tagcaatggg gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt
    33001 tctccttgga aatctggatt tctttttcga ggatctcctt caccaaattg tttttcctca
    33061 ccaagttcat attttcctgt cagcaaaccg gaagcaagag gaaaataggg aacaaaactg
    33121 attttgtttt tttcaagata agggaataat tctttttcag cctgccgatg gatcaaactg
    33181 tatttatctt ctactacatc gacatatcca tctgcattcg cttctttcaa ttgttctaac
    33241 gtgaagtttg acacaccgac tgctcggatt tttcctgctt cttttaattc gtgcaatgta
    33301 gcaactgatt cattttttgg tgtgctttca tccggaaagt ggatataaaa aatgtcgatg
    33361 taatcagttt gcagacgttt tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa
    33421 ttatcaaatg ttcgcccttt atttggtaca tgagcagctt ttgtagcgat gacgacacgt
    33481 gagcgatcat attctttttc cttcagtacc tcgccaatca gttcttcgga acggccgttt
    33541 ccgtaagcat aagcggtatc gatcaaattg atcccgctat tcaaagctgt acgaactact
    33601 tgttttcctg tctcatcttc caaaccgtca aaaagattat gtcctccaac ggcatttgct
    33661 cctaatccaa gctgttctgc gtagacttgt gaatgtccga ttcttatttc tgccatagct
    33721 gattcctcct ttttgtctag tctaaaacaa aataccaaaa agctgaacta aaacgttttt
    33781 gtgaggggaa atagtcagat atctcttgac tttgagactg gtttttagta ggttataagt
    33841 aatatcgaat gtcaatgaaa gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc
    33901 gaggctgaaa atgatctaaa gtttcattag cctaaagaca ctctggagat ggacgagtga
    33961 aaagccgtcc cgcgtacgag cgttattcgt aagaggaaag agaaatcttt caaaattagg
    34021 tggtaccgtg tatgaatttc attcaccctt gttcatgatg aacaagggtg atttttttat
    34081 ttacatagct aagaaaggga gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg
    34141 atattttgcc aggcgaatca gaaaaatggc agtttgtcga agaaacagca cgtttattat
    34201 tcagagatta tcagtataat gaaatgcgta caccgatttt tgaacactat gaagtgatat
    34261 cccgaagtgt aggggataca acggatatcg tttcaaaaga gatgtatgat ttttatgaca
    34321 aaggggaacg ccatgtgacg cttcgaccag aaggtacagc accaatcgtt cgttcttttg
    34381 ttgagcataa attgttcggc ccagagtatg cgaaaccgtt caaaactttt tatatgggac
    34441 caatgtttcg ctacgaacgc cctcaaaaag gacggttacg tcaattccat caaatcggag
    34501 tagaagcatt tggatcagaa aaccctgcaa ctgatgtgga gagcatggtg atggcacttg
    34561 atttcttcaa acagttgggg atcacgcaaa tccggctagt catcaattca ttaggtgata
    34621 aagaaacgcg tcaagcctat cgtcaagcat tgatcgacta tcttttacct tttgaatcag
    34681 agctgagtga agactctaaa cgccgccttc atgaaaatcc attacgcgta ttggacagta
    34741 aagacaaacg agatcaaaaa tttgtggcag aagctccatc tatcttagat tatctaagcg
    34801 agcctgctca aaaacatttt gataccgttg tttccatgct ggatgcattg gctgttcctt
    34861 atgaaatcga cagtaatatg gttcgcggac tagattatta cacacatacg atttttgaaa
    34921 tcatgagtga cgcaccaaaa atgggggcac aagcaacgat ttgcgcaggc ggacgttatg
    34981 acaatctagt ggaagaacta ggtggtcctt caacacctgg cttcggattt gcaatgggta
    35041 tcgagcgatt actcttgact atggaggcag aggaagtagt tatccctgca tttaatgagt
    35101 tggatgctta cgtggtcgca ctcggagacg aaacaaatat cgaagcattg aaagtggtcc
    35161 aagcgattcg aaactttggt ttttcagctg accgcgactt catgaaccgt aaagcaaaag
    35221 cgcaatttaa gacagcagat aaagcgaatg ccaaattagt attggtgatc ggtgaagatg
    35281 agcttgctag tcaaacgatt aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc
    35341 cattagaaga gatatatgaa cattttgatg aagtctatga tgaaatgact ttattcgagt
    35401 aggaggaaaa aaataaatgg cacaaagaac gatctattgc ggattagttt ccgaacaaca
    35461 attagaacaa acagttacat taaaaggatg ggtacaaaaa agacgagacc ttgggggagt
    35521 aatctttatc gacctacgtg atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat
    35581 caacaaagaa gcatgggaaa tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac
    35641 tggggaagtt agaagacgcg gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt
    35701 cgaagtgatt gcaagtgaaa tcgtgatttt gaatcaagca aaaaccccgc catttcaaat
    35761 tgaagatgat caagcaatca gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg
    35821 tcgtccaaaa atgactaaaa acatcaaatt aagaaatgcg acaacaaaag cgattcgtca
    35881 ttatttagat gataatgact ttctcgatat cgaaacacct tacctaggga aatctacacc
    35941 agaaggcgca agagactacc tagttccttc acgtgtccat gctggacatt tctatgcgtt
    36001 gccgcaatct cctcaattgt ttaaacaatt gctgatgaat gctggattcg accgttatta
    36061 tcaaatcgtt cgttgtttcc gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac
    36121 gcaagtagat attgaaacaa cgttcttaac agcagaagaa atccaaactt acacagaggg
    36181 attgattgcg aaggtaatga aagaagttcg cggtatcgaa gtcacattgc catttcctcg
    36241 tatgacctat gatgaagcaa tggctcgcta cggcagcgac aaaccggata cacgttttga
    36301 tatggaattg atcgatctaa gcgatacagt caaagaagta gaattcaaag tcttccaaat
    36361 ggctcttgaa aacggcggtg tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta
    36421 ctcacgaaaa gacatggatc agttaggtca atatgtagga caattcggag ctaaggggct
    36481 tgcatggctg aaagtagaag aagacggatt gaaaggtcca attgctaaat tcatgggaga
    36541 agcaacagaa gcaatcatta aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg
    36601 cgcagataaa agtgaaatcg tcgctgctgc attaggtgct atccggacac gtttaggaaa
    36661 agaattagga ctaattgatg aatcgaaatt caatttctta tgggtaactg attggccaca
    36721 atttgaattt tcagaagaag aaggtcgata tgtctctgct caccatccat tcacgatgcc
    36781 aaaagaagaa gatatcccgt tattggctga agaccctgca aaagtctatg cagaagctta
    36841 tgatatcgtc ttgaacggtt atgaattagg cggaggttct atccgtattc atactcgtga
    36901 gcttcaagaa aaagtactag aaactctagg ctttacaaaa gaacaaatgg aagaacaatt
    36961 tggtttcttg ttaacagctt tagattacgg attccctcca catggcggta ttgcattagg
    37021 attggaccgt tttgcaatgt tattagccgg agaagataat attcgtgaag tcattgcctt
    37081 tcctaaaaac ggaaaagcag cagatgtgat gagtgatgca ccaagtatgg tttcgccttt
    37141 acaattgttc gaattgaata ttgatgtaac gaatatagaa gaatagccga taaaaaggta
    37201 aaaaggattt gtcgaatacg cttcttttga caaccttgat ttaattgaaa aacactttat
    37261 aatgaagcca taacatataa agcggggctt ttccatgaaa aaaacattcg aaaattgggc
    37321 ttatcatgat tacacgacca agctttcggt atctatcgtt tatgcgttga tcgcatcagt
    37381 cgcattgaat ttcttttatc agccaggaca tatttattca agtggtatta ctggtctagc
    37441 tcagattttg acgactttgt caaaatcact gataggattc gaagtaccgg tatcaacaac
    37501 tttgtattta ctgaatatac ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt
    37561 tacaattttc acttttatca cagtcacttt gacctccctt tgtatccata ttttaccgga
    37621 aacagtccta tctaccgatc caatcatctg tgcgttgttt gggggggctg tgatgggact
    37681 aggtattggg ctagcgttga gaaacggcct atcatcgggc ggtctagata tcgtgagtat
    37741 cacgattcgt aaaaaaacag gaaagtcgat tggttctatt tcgatctact tcaatgcatt
    37801 gatcgttttt gttgccggaa ttctatttgg ctggcaatat atgttttaca gtacgttgtc
    37861 tattttcgtc agtggaaaag taactgatgt cgtttatact aaacaaaaga agatgcaggt
    37921 aatgatcgta acgaaacatc cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag
    37981 agggatcacg atcatcaatg aagcagaagg tgcttataag catgataagc agacagtatt
    38041 gatgacgatc gttacacgat acgaactgcc tctacttaga gaagcaatga aagcttctga
    38101 tccacaagct tttgtaagta ttgcagaaaa cgtccagata cttggtcgtt tctatgagga
    38161 tggcatttag atcgagtttg acaaaaattc atgaaatact gctgtagaca gacgttcgtt
    38221 tgtctgcagt ttttttctac catagtagaa tcagcaaaga aaaactgctt ttttatttga
    38281 aattttctta aagttgaaaa aaagcatgtt tattttttta aatttatggg atgatagaaa
    38341 aaaatagtag agaaaggaca cgattctact tgctattttc acttaatacc cgtatattac
    38401 gaatgaaaaa agtttatgaa aagttggaat attgaatgga aaaacaaaaa aggttaaaaa
    38461 acagccgcta catcactggg tttgatgggc tccgttcatt agctgtggtg ggtgttatct
    38521 tctatcacct tctgccaact agtatgaaag gcggctattt gggcgttccg atttttttcg
    38581 tcgtatctgg ttatttgatc acggatctgc tgcggcagga atgggaacaa aatggaaaaa
    38641 tcaatatttg gcaattttat gtcagaagaa tgaagagact ctatccggca cttgttttta
    38701 tgcttgtgac agcttcggct tatatcactt tgtttcagcg agggctgtta aacaatcttc
    38761 gaggagttgt ggtcagcagc ttactgtatg tcaacaactg gtggcaaatc aataacggtt
    38821 tatcttattt cgatcgattt gctaatgagt caccgtttac gcatatctgg tctttagcag
    38881 tagaaggtca aaactatttg gtttggccga tcttgtttgt attgttgatg gtatttgttc
    38941 gtaaaagaaa atggatcttt tatactgtgt taggtacatc cttcctctcc gccatcttga
    39001 tgatgatctt gtatacacca ggaggcgatc caacccgtgt ttattacggt accgacactc
    39061 gtctgttttc tatatggttg ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa
    39121 agaaaaacat cccgaaacaa gcgaaaaata ttctgaatat cgctggagga atctcgcttt
    39181 tggcactgat tctttgcttc ttctttttag atgatcatta caatttcatc tattacggag
    39241 gaatgctcct agtgagtctg ttatgcgtag tgctagttgc agtgacagca caccccggag
    39301 caagtttgaa tcgatggctg acaaatccag tatttacttg gattggcaaa cgaagctatg
    39361 gcatttatct gtatcagttt ccagttatga ttttttatga agcaaaggtg actgatattg
    39421 ccgatcatgt tttgttgcat actttagtgg aaatcacatt gattctaatc atcagtgaac
    39481 tttcttatcg atatgtggag cgtccgctag cacgttttga ttatggtcgt acattccaag
    39541 tgatgaaagg atggttcagc aaaccgatca tcagtaagag caaaccatgg gttgttccag
    39601 gcacattact tgtagcaatt gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa
    39661 cagccgatca aaaacaactg caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa
    39721 ccagaaacaa ttcagaaaac accactagtt cggttgacca agcgatcctt gataagtacg
    39781 agttgtccaa ggcacaaggg aataaggcac aaaaattaga actaacggca tttggtgatt
    39841 cagttatgtt ggatgctgca acggatctac aagaacttta tccaaaagta gtggtagatg
    39901 gagatgtcgg acgacaatta tatgccagtg taccgtacat tgaaaaatta aaggaacaag
    39961 atttgttgaa agacactgtt ctgattggac taggaacgaa tggctcattc tcggaagctc
    40021 agtttgatag tgtgatggat gcaatcggtg atcgaaaagt atactgggtc aatgtccgag
    40081 taccgacgaa gcgctggcaa aatgacgtga atgcgatgct tgaacagatg acgaaaaaat
    40141 acgacaatat gacgctgatt gattggtaca gctacagtaa tgaccatagt gactggtttt
    40201 atgatgatca agtccatcca aatcctgaag ggatgacaca ttacattcac ttagtcagcg
    40261 aagccttgct aggaggaagt tcaagtacga acagcagtga gacaaacgtg acagaatgat
    40321 agctacaaaa aacttcaggc aaaaacattt gtttttgcct gaagtttttt tatatttacc
    40381 gtgtttttaa cattcatata gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac
    40441 gttctttttg ttttcgggtg agttttttga atgcagcttc tgcttttgtt gcttcgcttc
    40501 ttgaagaaaa tacttctgca tggatcatct taacaggtct tcttttttga agacgagtat
    40561 atttagcacc agtaccattg ttatgttcct ctaatcgtct agaaagatcg gttgtatatc
    40621 caccataaaa tgtctgatct ttacagaata atacgtaaaa ataatgttca tttgccatag
    40681 agcatctcac gaatctctgc agtgtattct ccatccatat tgtaagtgat caatggtgga
    40741 gcgactttga atccgtctaa tttgccttct ttgattcctt caactaacaa aatatttgcc
    40801 tcttttccag gttttgggta aacaaattgg atccttttag gtgcaattcg atagtcttcc
    40861 atgacctgaa tgatttctaa gaaccgatca ggacgatgga ccatagtaaa gcgtccattt
    40921 gtttttagta gcttacttga aatatcgatc acttcttcta acgtcgtatg aatctcatga
    40981 cgggcgattg ccaggtgttt attagggttc gttttatttg ttggcagccc tttgaaataa
    41041 ggtggattac agacgactaa gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc
    41101 agatcgatgg tgtggacagt catttgttca tttaaatggt taagcgcgat actacgtctt
    41161 gccatgtctg ctagacgagg ttgaagttcg atagcgtcga tagccgcctg ggttcgttta
    41221 cttaaaaaaa gtccaactgc gccatttcct gcacaaaggt caacaatctt ccctttttta
    41281 ggtacacggg aaaagtgagc aagcagcaca gcatctaaag aaaaagaaaa cactttggaa
    41341 ctttggataa tcttgatatc ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc
    41401 atggattccc tccaaaatga aaaaaatagt cgtctgtatt atactatttt tattaaaaag
    41461 aaccaaggtg gcttgtcaaa cctctatttt ttatgcatac tatgaccata agaagggaaa
    41521 ggaagagact atgttttatc gatttatgcg cggggtagtt cgagtactat tagccttagt
    41581 taatggaaat gcccgctatg agaataaaga agttttaccg caagatgaga attacatact
    41641 agttgctccc catcgtactt ggtgggaccc attatatctg gcggtagctg cagcacccaa
    41701 ggaattcagc tttatggcaa aagaagaact gttcaaaaat ccagttctgc gatttatatt
    41761 aactcgatcc aatgcttttc cagttaaaag agaaaatcca ggaccaagcg tcattaaaac
    41821 acctgtaaag attttgaaat cgacaaattt gagtttgatc atgtttccca gtgggacaag
    41881 acatgctaca gaacttaaag gcggaatggc attgatttcc agaatgtcta aagcgaaaat
    41941 cgtgccagct gtatatcaag gtccgttgac acttggggat ctgttcaagc gtcagagagt
    42001 gacagtgcgt ttcggcgaac caatcgactt atcagatatc aagaaaacgg ataaagaagg
    42061 tatggcggag atcgaacgac gaacacaagc agcttttgac aaattggaca aagaagtgaa
    42121 ccctgatttc aaatatgaag tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc
    42181 ttttgaatct actgtcctgt tcagttcaga ctttttcaat gaaaacgagg gagagacgtc
    42241 aagtctgccc tctttttgcg ttccaatatg taaaatagaa gacaggtgtc atgtaatgga
    42301 tcaaagtgtt ataataatta gaaggacatt ttttgaggtg attgaatgcg ttttttacat
    42361 acagccgatt ggcatatcgg aaaaaaacta caaggttatg acttgattga agaccagcgt
    42421 catgtactat cttctatact agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt
    42481 gccggagact tatatgatag gagtgtccca gctgttgatg ccgtagaatt atacaatgaa
    42541 ttgatggtcg attggaattt aaaggaaaag atcccgattt ttgctatatc aggaaatcac
    42601 gatagcagta cgagactttc agcgggtacg ccatggttca gttattcgaa ctattttttg
    42661 cgaacacagc tagcagaagc atttgaacca gtcgagttta aaaacgtaca atttttcttt
    42721 cttccttact ttgaaccaat tgcagcgaga ttatattttg aagatgacaa tatccgaacg
    42781 atcaagcaag cggtagccaa ggtgatagat aaaatgcagg agctatttga ttcagaaaaa
    42841 aaacaagtat tgattagcca tttttttgta atgggaagtg taaaatctga ttcggaaacg
    42901 aaaatcgaag taggcgggtt ggattcgatc cctggtgaac tacttacacc gtttgattat
    42961 gtagcactag gacatttgca taatcaagct gctttgagac aggataatgc tcgctatagc
    43021 ggttccccat tgaaattctc tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt
    43081 gacctgacaa caggcctgga gttgaatttt agggagatcg aacccctgca tgatattgaa
    43141 gaaataacag cgagtttcaa agaattgctg gatcctgcct tttatcagtc cgtcaatcgg
    43201 gagaattaca tctatatcta tctcactgat agaacgatca tcccgaatat gatgaaccag
    43261 cttcggcaaa tatatcctcg tattttgggg attgaacggg caaacggtcg agcagaacaa
    43321 acaaagcgca aacgaaaagt actagaaatg aaagatcctc aaaaattagc agaagcattt
    43381 tttagtgaaa tgacggaaga gcagctaact gcaaaccaag aaaaatggct cggagaaacg
    43441 ttgcaagaaa tcatggaaat ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa
    43501 aattttggtc cttttatcaa tgagaccgta gatttttcac gtttgacaga agcaccgctt
    43561 tttttgatca gcgggaagac aggagctggc aaaacgacga tttttgatgg gatcaccttc
    43621 gcactttttg gtgaaacttc cggaagactt cgctctggaa aagagatgcg ttctttgttt
    43681 gctacaccag acgaagaaac aagcgtgact ttttcttttg aacatcagca gatgagctat
    43741 gaaatcgaac gaaaaccaga acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa
    43801 caggcagcca aagtaacgtt gaccattttt gatgggaaag gaaaagaaat aaaacaaatc
    43861 acaaaacgta cagaagcaga tcagttgatc agagaactga tgaatctcga tgcgaaacag
    43921 ttttctcaaa tcgttttact accccaagga gaattccgta attttttggt ttcatcaagc
    43981 agtgataaag agactgtttt acgtcattta ttcggtactc aatttttcca acagttcaat
    44041 gagcgattaa aagaaaaagc aaaacaacag caacaaaaat tggatcattt agagcaagag
    44101 ctgcatcttc tgcaaaaaag gtttgtttta gtcaaagaag aggaaccagc agcagggttt
    44161 gaagccgttt tagcccaatg gacaaaccat caggcaatac tcgaaaaaca aatcacggaa
    44221 gaaaaagaag ctctaacgga acttcaggaa aaacaaaagc aactagaaaa ttcctactat
    44281 cgttttgaaa gcttgaaaaa aagttatgaa gaaaaaacga tattaataaa caagcaaaag
    44341 gaattagctg ctcaaaaaga cgaaatagag gaaaagaaac gatggattgg gtattatgaa
    44401 tttacgcaac gattagtcga accaatccag cgcctacaag aaataaaagt tgaaaaaata
    44461 gaactgtcac aaaaaagtag tgagcaaaca aagaaactag cgatgactac gcagaattat
    44521 gaagagtggc aaaaaaatga agcaaaccgc gtacgccgac aaacaaaaat caaagaagct
    44581 gctcaaaaac tgcaaaacaa acagatgatg gtgccaattg tcgaatcctt acgtagtaaa
    44641 aaacaagacg cagaacaatt aacaatttca ttggataaag aagaattgaa tgccgagaag
    44701 ctgtatcaaa atctcgctat ctatcaggaa agaactcgaa ttcttcaaga aaatttactg
    44761 gatcaggaaa aactgcaaga agatcgtctt gcttttggac agttaaaata tctagatgaa
    44821 aagcagcaag aaatagaaaa acaaaaagca aaagacactg ggtcgctcgc acagataaga
    44881 gaagaactca aaaaagcaac tgaagaaata acaaaaaata gtcagctatt gaaagaacag
    44941 acagaacaag cagaacagct gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag
    45001 ctttttttgc gtccagggga gccatgtctt gtttgtggat caacggaaca ccccatacaa
    45061 caattaaaac acgaacagcc aaataaggaa gatatcttaa aagtagaaca acaactggca
    45121 gaagctgagt ctgctgtaga aagaactcag caaatcattg ccaaaagtga agcacatgaa
    45181 aataatcttc aacaacaatt atcagattta gaacaaaaaa tcatggcaac gaatatacaa
    45241 ttggctgaac aagaagaaca cttgcaacag gctttgaaaa aggcatttcc tgagataaca
    45301 gaacaaaaaa caaaaacaac tcttgtagta atcgaacaat ctttagatag acgaaaaagc
    45361 aagatggcca atgcaagaat agagctggaa gaaataagac aacaagcaga aaagctcgaa
    45421 caacaattag ctgtctacaa acagcagcag acagaaaaaa ataatcagct ccagcaactg
    45481 caaggagaaa tcaaaagttt agaacaacaa ttgcaagata cggatagtaa ctgtttagaa
    45541 gaggagatac gacgacttga aaaacagtta gaagaagact cgaatcaact agctgttgag
    45601 cagaaagcag gagtgcaatt gttgcaagat cttcatacgc ttcggacaca gcttgatctt
    45661 caacatgaac aaatagaaaa aatagatgaa aaaatagaaa aggaacaaga aagaatagct
    45721 aaaaagttag aaagccagtc gtatttttca acaattgaag aaatctgtgc atttgcacaa
    45781 cagcaagcac aatataaaga aaaccaacgt acagttactt cttatcatga agagcaatta
    45841 gtgacagctg accggctgcg tcaattagaa aatatccagc taactgagac attcccagat
    45901 acagaagaga tcagccaaca gttagaagaa ataagacgta tgatcgaaat aaagcaagaa
    45961 aagatatacg gtttacaaga acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt
    46021 tcgatttatc acacaagcca gacgaaatta gatgagctta cgcaattaca gcagctttcg
    46081 caaacattca acggagaaaa tcctaagaag actagtttgg aacggtatgt gcttcaggtt
    46141 tatcttcaag aagtgttaca agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt
    46201 tatcaatttg agctagcgga taccattggc agttatcgag ggaaaaccgg attagaaatc
    46261 aatatctatg atgatcaggc aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag
    46321 agttttattg cagcactttc tttagcatta gctcttgccg atgtgatcca gacacaagct
    46381 ggtggtgtag caatcgatgc attgtttatt gatgaaggat ttgggtcatt agatgaagaa
    46441 gctttggaaa tggcaatgga agctttagaa acgatcgaaa gtgaaggaag aatgatcggc
    46501 atcattagtc atgtaagaga attgaaagaa cgagtattac aacaaataag aattgataca
    46561 gaaggtagcg gacaaagcaa aatacgctat caacttggat aaaaagggag attggtaact
    46621 atgaaatgga gtattccaga aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc
    46681 cgagtgttat cggttgtccc tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc
    46741 agtgaattgt atatggttga tttggatggt acagcaaaag agatagatta ctgccaatgt
    46801 ccctattggg aggaacataa atattgtaag catacagttg ctgtggaact ttatttacgg
    46861 tcgaaaaatc tatctcgtat catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca
    46921 acaagtgagg gagaaatcat tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct
    46981 caagcggtgc aacctttgat tattgaatac caagtagaaa cgattgagac taatcagtat
    47041 cacccagagc tttctatttt ggcactttac ttgcgtatcg gttcattggc atcaccgaaa
    47101 aaaacatata tcgtaaaaaa catctatgat tttttacaag cttacacaga aaaagaaact
    47161 tacactgtta ataaacagta ccagtttcgt ctagataaac gggcgtttca aaaagaagat
    47221 caaaaaatac tcacgcatct tgcaggaagt gctcagacgc atcaattatt aggtacgaac
    47281 ggcttacaag tcaaaggaaa actagataaa agatatctgc ttctcccagt agaacaagcc
    47341 cgcttcctgt tagagcagat gaatcagact acacgtttgc atctgcagat aaatgaacaa
    47401 aacgatcaag gaatacaatt ttccgatcaa aacaaacctt tatcattcaa agtggaaaaa
    47461 tttggagaag attatttgct gcaagcattg aatgattttg attttttctt tatgcattat
    47521 cagtggggag ttatccaagg gacattttat tctctgacga aatatcaaca aacggtttat
    47581 caaacttgga aacaattgat tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa
    47641 gaattaccgt ctttgtttaa agagattatc cctctgctgt cagagatcgg cgacgtagct
    47701 attgcccctg cagtagaaga ggaagtagca gatatccccg tcgaatttat tttctttttc
    47761 cgaaaagcaa aaggaaaaat ccaagctcga gtcgatttcg tctatggtga agtcatctat
    47821 tcgactgacc cgaagcatga ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca
    47881 gtagaagaga aacgagtact tgatctattc agaatgtatc gctaccagaa aaatgaaact
    47941 ggttatgaac gtccattgcc aagcggggaa gaactatatg cctttttccg cacagaacta
    48001 tctgtcttcc gtcagtttgg tgaagtcaga cttggaagaa aattgcgaga gttgtattta
    48061 gatgcgcata aacatcaacc gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt
    48121 cgattcgatg tgacagggat agaggagcaa gaaatcgatc atgtcctgca aagcttgctg
    48181 agaaatgatg ctttttatac attagaaaat ggtcaggttc tttcttttga ttcagaagaa
    48241 tttcaacaaa caagccaagt gttgcaacaa ttacgggaat cgatacgtac agaagaagga
    48301 acgatccatg tacctaaaaa tcaaggattg atcattcaaa atcaattgga aaaaagcaat
    48361 gctactttta gtgaatcgtt ccaaacgatg gtacaagatc tcattcatcc agaaagatac
    48421 caggcacagc ttcccaaagg attgaatgca acgatgagag attatcaaaa gcaaggtttt
    48481 cgatggttga agatgttagg tcattatcag tttggcggta ttttagccga tgaaatggga
    48541 cttgggaaaa cactacagac aattgccttt ttactttcgg aaaaagaaga acggaaaagc
    48601 ttctctgctt tgatcgtagc gcctgcgagc ttaatttaca actggcaggc agaggtgaga
    48661 aaattcgccc cttctttaag tatccaagta atcaatggga ataaaaaaga acgtgaagaa
    48721 ttactggcta aagacacaga tatccgagtg acttcttatg ccagcttaag acaggatcta
    48781 gctaattatc agtcgcaaaa aattgattat ttgattcttg atgaggcaca aatggtcaaa
    48841 aatagcagta cgaaaactgc acaagcattg agagaattag ctgtacctca gcggtttgca
    48901 ctgagtggga caccaatcga gaataattta gaagaattgt ggtcattgtt tgcaacgatt
    48961 atgccaggat ttttcccgac taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg
    49021 caaatgattc gaccgtttat tttacggcga gataaacaaa ctgtgttgaa agacttgccg
    49081 gaaaaaacag agatgaatct atattctgca cttacagaag aacaaaaaac tgtttattta
    49141 gcttacttgc gtcaaatgcg tgaagaaatc acatcaatgg attctgaagc attcaagaaa
    49201 aaccggatcg ggatacttgc tggtctgaca agactgcgac aaatttgctg cgatcctcgt
    49261 ctgtttattg aagattacca aggcggttcc ggcaagctcg aacaagtaaa agatctactg
    49321 gtagctgcca aagagaacaa acgaagagtt ttgctgtttt ctcaatttac agggatgctg
    49381 actatcttgc aagaagagtt agccgagcta gggatctcga cattttattt aagaggcagt
    49441 acgaaaccac aagatcggtt aagtatggta gacgcattta atgctggcga aaaagatgtt
    49501 tttctgattt ccttaaaggc tgggggaact ggattgaatc tcacgggagc agatacggtc
    49561 atcttgtatg acctttggtg gaatcctgcc attgaagaac aagctgctgg acgtgcacat
    49621 cggatcggac aaaaaaatgt ggtagaagtg tggcgtatga tctctgaagg aacgattgag
    49681 gaacgcatgg attcactcca gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt
    49741 aatgaagaac agttgacgaa actgactgaa gaagacatcc gcatgatttt aagtgtagga
    49801 gaaatcgagg agtaagagac tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa
    49861 agaagcgatt gtttatttga tttaaaaaat agcaaggagg ctgggattcc attttatccc
    49921 agcttccttg ctatcatttt tttatttcta tttttaaagg ttatacaaag attgtcttca
    49981 ttgtctctaa cagctgatca aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa
    50041 ttttgcgttc tcgatggttg aaccaaagcg cttgccagtt agcacttttt gcacctagaa
    50101 catcattgtc gtaattatcg ccaacgtata aggtctcttc tggaagcata tgaaattctt
    50161 tctcagccag ctgaaaaatc tctttttctg gtttttggaa acctgttgct tgagaaatga
    50221 gcatattgcg ggaaggaatc caattgttca attgaagttg cttcagtttt tttgtctgat
    50281 ggtcagtcgg cccatttgtg ataattccta atgggacatt cttttccttc aagaaatcga
    50341 gggttttttt gacctcctca tgcaagcaga tattatcaag ttcttcctcg taaatttttt
    50401 gaaataagag gccatcttct tccgtaatat gagggtagtc taaatctttt agtgattggc
    50461 tgatccgatg tgctcgcata tattctagtg tccaatctcc tgccattact tttgggaagt
    50521 tttcatcact atgatggcgg aaccggatat atagtggatg catatctgca tctgaaacaa
    50581 gcggaacgac tcttttgacc gcgtttcgaa atggctgctg ctgatcatac atcgtatcat
    50641 caacatcaaa cacaactgat ttaatcaaaa ttttacacgc ctttcggttt tcattgattt
    50701 tttcacaaat ccttgactat tgtaacagaa aaaattgtct aaaaaagctg ataaataaag
    50761 gtttttctta ctatatttct atttccttga cagaataagg tttttatatt tcagtttttt
    50821 tcactaaaaa tcgtgaaaca cgtcaataag tttgatgaac aaattaatca ttatggtaga
    50881 agtcgattga tttattttaa tgcaatctct gtggaatttt cgaaag
//
LOCUS       DAZKMZ010000011        50128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000011 DAZKMZ010000000
VERSION     DAZKMZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.011"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..268)
                     /locus_tag="ACG8M5_000753"
     CDS             complement(<1..268)
                     /locus_tag="ACG8M5_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0358031.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGI"
     gene            complement(270..467)
                     /locus_tag="ACG8M5_000754"
     CDS             complement(270..467)
                     /locus_tag="ACG8M5_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0358032.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(861..2588)
                     /locus_tag="ACG8M5_000755"
     CDS             complement(861..2588)
                     /locus_tag="ACG8M5_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0358033.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2581..3774)
                     /locus_tag="ACG8M5_000756"
     CDS             complement(2581..3774)
                     /locus_tag="ACG8M5_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0358034.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3961..4605)
                     /locus_tag="ACG8M5_000757"
     CDS             complement(3961..4605)
                     /locus_tag="ACG8M5_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0358035.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4699..4833)
                     /locus_tag="ACG8M5_000758"
     CDS             complement(4699..4833)
                     /locus_tag="ACG8M5_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0358036.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4835..6967)
                     /gene="feoB"
                     /locus_tag="ACG8M5_000759"
     CDS             complement(4835..6967)
                     /gene="feoB"
                     /locus_tag="ACG8M5_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0358037.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6964..7437)
                     /locus_tag="ACG8M5_000760"
     CDS             complement(6964..7437)
                     /locus_tag="ACG8M5_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0358038.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7838..8062
                     /gene="nrdH"
                     /locus_tag="ACG8M5_000761"
     CDS             7838..8062
                     /gene="nrdH"
                     /locus_tag="ACG8M5_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0358039.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8221..10380
                     /gene="nrdE"
                     /locus_tag="ACG8M5_000762"
     CDS             8221..10380
                     /gene="nrdE"
                     /locus_tag="ACG8M5_000762"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0358040.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10430..11395
                     /gene="nrdF"
                     /locus_tag="ACG8M5_000763"
     CDS             10430..11395
                     /gene="nrdF"
                     /locus_tag="ACG8M5_000763"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0358041.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11484..12623)
                     /locus_tag="ACG8M5_000764"
     CDS             complement(11484..12623)
                     /locus_tag="ACG8M5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0358042.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12932..13645)
                     /locus_tag="ACG8M5_000765"
     CDS             complement(12932..13645)
                     /locus_tag="ACG8M5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0358043.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13899..14600)
                     /gene="nagB"
                     /locus_tag="ACG8M5_000766"
     CDS             complement(13899..14600)
                     /gene="nagB"
                     /locus_tag="ACG8M5_000766"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0358044.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14834..16366
                     /locus_tag="ACG8M5_000767"
     CDS             14834..16366
                     /locus_tag="ACG8M5_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0358045.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16557..17759)
                     /locus_tag="ACG8M5_000768"
     CDS             complement(16557..17759)
                     /locus_tag="ACG8M5_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0358046.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17841..18530)
                     /locus_tag="ACG8M5_000769"
     CDS             complement(17841..18530)
                     /locus_tag="ACG8M5_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0358047.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18636..19004)
                     /gene="rplL"
                     /locus_tag="ACG8M5_000770"
     CDS             complement(18636..19004)
                     /gene="rplL"
                     /locus_tag="ACG8M5_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0358048.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19057..19560)
                     /gene="rplJ"
                     /locus_tag="ACG8M5_000771"
     CDS             complement(19057..19560)
                     /gene="rplJ"
                     /locus_tag="ACG8M5_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0358049.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19764..19891)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19970..20659)
                     /gene="rplA"
                     /locus_tag="ACG8M5_000772"
     CDS             complement(19970..20659)
                     /gene="rplA"
                     /locus_tag="ACG8M5_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0358050.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20763..21185)
                     /gene="rplK"
                     /locus_tag="ACG8M5_000773"
     CDS             complement(20763..21185)
                     /gene="rplK"
                     /locus_tag="ACG8M5_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0358051.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21528..22196
                     /gene="sdaAB"
                     /locus_tag="ACG8M5_000774"
     CDS             21528..22196
                     /gene="sdaAB"
                     /locus_tag="ACG8M5_000774"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0358052.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22209..23081
                     /gene="sdaAA"
                     /locus_tag="ACG8M5_000775"
     CDS             22209..23081
                     /gene="sdaAA"
                     /locus_tag="ACG8M5_000775"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0358053.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23172..24272)
                     /locus_tag="ACG8M5_000776"
                     /pseudo
     CDS             complement(23172..24272)
                     /locus_tag="ACG8M5_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24364..25251)
                     /locus_tag="ACG8M5_000777"
     CDS             complement(24364..25251)
                     /locus_tag="ACG8M5_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0358054.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25328..25873)
                     /gene="nusG"
                     /locus_tag="ACG8M5_000778"
     CDS             complement(25328..25873)
                     /gene="nusG"
                     /locus_tag="ACG8M5_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0358055.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25974..26144)
                     /gene="secE"
                     /locus_tag="ACG8M5_000779"
     CDS             complement(25974..26144)
                     /gene="secE"
                     /locus_tag="ACG8M5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0358056.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26168..26320)
                     /gene="rpmG"
                     /locus_tag="ACG8M5_000780"
     CDS             complement(26168..26320)
                     /gene="rpmG"
                     /locus_tag="ACG8M5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0358057.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26482..27126
                     /gene="cbpA"
                     /locus_tag="ACG8M5_000781"
     CDS             26482..27126
                     /gene="cbpA"
                     /locus_tag="ACG8M5_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0358058.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27182..27685)
                     /locus_tag="ACG8M5_000782"
     CDS             complement(27182..27685)
                     /locus_tag="ACG8M5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0358059.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27698..27744)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28282..29247
                     /locus_tag="ACG8M5_000783"
     CDS             28282..29247
                     /locus_tag="ACG8M5_000783"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0358060.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29346..30518)
                     /locus_tag="ACG8M5_000784"
     CDS             complement(29346..30518)
                     /locus_tag="ACG8M5_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358061.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30505..31650)
                     /locus_tag="ACG8M5_000785"
     CDS             complement(30505..31650)
                     /locus_tag="ACG8M5_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358062.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31664..32588)
                     /locus_tag="ACG8M5_000786"
                     /pseudo
     CDS             complement(31664..32588)
                     /locus_tag="ACG8M5_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32705..33784
                     /locus_tag="ACG8M5_000787"
     CDS             32705..33784
                     /locus_tag="ACG8M5_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0358063.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33781..34409
                     /locus_tag="ACG8M5_000788"
                     /pseudo
     CDS             33781..34409
                     /locus_tag="ACG8M5_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34519..34746)
                     /locus_tag="ACG8M5_000789"
     CDS             complement(34519..34746)
                     /locus_tag="ACG8M5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0358064.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34849..37437)
                     /locus_tag="ACG8M5_000790"
     CDS             complement(34849..37437)
                     /locus_tag="ACG8M5_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0358065.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37457..38116)
                     /locus_tag="ACG8M5_000791"
     CDS             complement(37457..38116)
                     /locus_tag="ACG8M5_000791"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0358066.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38165..38668)
                     /gene="trmL"
                     /locus_tag="ACG8M5_000792"
     CDS             complement(38165..38668)
                     /gene="trmL"
                     /locus_tag="ACG8M5_000792"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0358067.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38889..39755)
                     /locus_tag="ACG8M5_000793"
     CDS             complement(38889..39755)
                     /locus_tag="ACG8M5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0358068.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39864..40505
                     /locus_tag="ACG8M5_000794"
     CDS             39864..40505
                     /locus_tag="ACG8M5_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0358069.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40567..41928)
                     /gene="mgtE"
                     /locus_tag="ACG8M5_000795"
     CDS             complement(40567..41928)
                     /gene="mgtE"
                     /locus_tag="ACG8M5_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0358070.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41946..42845)
                     /locus_tag="ACG8M5_000796"
     CDS             complement(41946..42845)
                     /locus_tag="ACG8M5_000796"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0358071.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42847..43644)
                     /locus_tag="ACG8M5_000797"
     CDS             complement(42847..43644)
                     /locus_tag="ACG8M5_000797"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0358072.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43622..44326)
                     /locus_tag="ACG8M5_000798"
     CDS             complement(43622..44326)
                     /locus_tag="ACG8M5_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0358073.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44457..45033
                     /locus_tag="ACG8M5_000799"
                     /pseudo
     CDS             44457..45033
                     /locus_tag="ACG8M5_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45151..45828
                     /locus_tag="ACG8M5_000800"
     CDS             45151..45828
                     /locus_tag="ACG8M5_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0358074.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45907..47715)
                     /gene="pepF"
                     /locus_tag="ACG8M5_000801"
     CDS             complement(45907..47715)
                     /gene="pepF"
                     /locus_tag="ACG8M5_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0358075.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47891..48889)
                     /locus_tag="ACG8M5_000802"
     CDS             complement(47891..48889)
                     /locus_tag="ACG8M5_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0358076.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49115..49768)
                     /locus_tag="ACG8M5_000803"
     CDS             complement(49115..49768)
                     /locus_tag="ACG8M5_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0358077.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15003 a        10513 c         8787 g        15825 t
ORIGIN      
        1 ttattccagc agttcttcca atttcactca aatttcttat taattcctct tgatattcag
       61 tgaatcttat tttattagta taagcagtaa aatatagata attcaccaat ctatttatta
      121 aaggaccggg atctaattca tatttttgtt tttctaattt taaatatacc ttttgtaaaa
      181 cctcaagaat atcagaatat ttaggatctt tacttataga tatatcatta tataattcat
      241 gaacaatttg cttagcattt tttttcattt tagcacttcc ctggaattgc tccacctaaa
      301 aaaccaccta tagacattcc tgcaatacaa gtagttgcct tggcccaatc gaccgtacat
      361 ttatttttag tgcaatacac tccatttcca taatattttc cactatgagt ggtaccccca
      421 tagataagtt gtgtctcttt aatagacaaa atttttaaat gtttcataat ctaatcaccc
      481 ctaaattaat gtttttagac tattattcta acattcatta aatttcaaaa aacaattcct
      541 tcttgaaacg tagcaaaata attcctgaat aagaatttaa tcacttttaa gtatataaac
      601 tatgtatatt ctttaatatt ttttgagaat gcacttcgta cataataaac tttaatcaca
      661 ttagtctttc atacctacga tcgatctctt tgactcgcac ccataaattc ataatagttt
      721 gtcggcgtct ttggacgttt cgaagatgag ctacgcattc tcttttttta tagacaaaaa
      781 agatagagac gaacatggag cttccaaatt tggatatttg atccaatttt ggaagctcca
      841 tttatttcgt gtctgtcttt ttattttttc cagctttcga ctaattcttg gttctctttg
      901 atccagtctt ttgctgcttg ctgtggatct tttccattgt tgatttccaa cataactttt
      961 tccatatctt tttctgacca atggaaattg tctaatactt tgtaagcttc tggctggtct
     1021 tctttcaatc cttttcgaac catcgtatgg atctgctctt cgcttcccat tccgttttca
     1081 ggatcttcta agtatttcag atcatatttg gcaaacatcc aatgaggtgt ccatcctgta
     1141 actacaattg gttcgtgttt tttgatcgct tgacctagtg caacggtcat tgctcctgaa
     1201 gaagatgtct ctactttcca gtctttcaag ttgtcatatt tttggatcgt gttttcagca
     1261 gccgtaacta caccagctcc tggctcgatt cctatgattt tcttacctgc ctgatcggtt
     1321 aactcatcga tcgaatttac atccatgtag gctggcactg ctaaaccaac ttttgctcct
     1381 gtcaaattgg cacctaggtc ttcgacttga tctttatatt gtgcatattg tgaaccatga
     1441 gttttaggta gccaagctga aaccatggca tctgactcgc catttgaaac agatttccac
     1501 atgattgaat tatctaatgg cgtcatcgtt acatcatagc ccatttgttt caatacttca
     1561 ccgactacat ttgttgaagc aacttctgta tcccattcaa cataagataa gttgatacgt
     1621 tttgtttctt tggcagatga aaaactccct ataccaatcg cacctaagat cactactact
     1681 acagcaccaa tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc tcctgcagct
     1741 tttttattgt ttggctggtt caaatgctgt gtaaaacggt caatgatgat tgctaagata
     1801 actaatgcaa ccccgttgac aaatccattc ccgacttgtg ctctttgcaa tgctgataat
     1861 acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa taacgaccat tgaaagtgac
     1921 agcattgttg tttgattgat ccctgccatg atcgtacttt tagctaaagg caattctagt
     1981 ttgaatagtt tttgacgact tgtgctcccg aatgaatcag aagcttctac taattcttta
     2041 ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg gtggcagagc aaagatgact
     2101 gaggcaaata cccctggaac catcccgata ccgaaaaatg caactgctgg aatcaaataa
     2161 acgaatcctg gcattgtctg cataaagtca aggatcggtt tgataatact ttgggcttta
     2221 ctgctttttg ccatcaagat ccctaatggt accccaatga tgatcgagat cacactagat
     2281 aaaagaacaa gtgtgacagt attcatcaaa tcattccata gaccttggtt ataaataaac
     2341 catagaccga ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat aaagaaagct
     2401 gctaccgtca ataacaagat aaacaaagga gctggaatca ctaacagtaa atttgtaatc
     2461 cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga aaaagttgtc
     2521 gtaaaccatt ctgtgatatt ttctacccag tttgccactg gtaattgata gttcaataga
     2581 ttattcatgt tcactcacct cgcttgtttc tgctaatgct tcaatcacac tacctcggat
     2641 gacgacacct tttaattttc catcctctac aactgcaagt ggagtcggtg agtcataaat
     2701 aatattgaaa atatcactta ccaccatgtc tttcccaatc accgtcacat tttcatctag
     2761 gaagtcgatc aatggacgtt tttgcttcct agcttctaac gcttgatctg cagtgatgat
     2821 ccctttcagg tgtctctttc tgtcaacagc catcagcatg ctgacttctt cattacgcat
     2881 ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg caggtaccat
     2941 gatgttttga gccgtaagca ctttggaacg atcgacttct tccacaaatt cacgtacata
     3001 atcatttgct ggattggtca atatttcttc acctgttccg atctgcatga tttcgccgtc
     3061 tttcatcaag gcgattctgt ccccaatccg taaagcttca ttcaaatcat gggtaatgaa
     3121 aataatcgtt ttttgaacat tggcttgcag gtccagtaat tcgtcttgca tttctcgccg
     3181 gatcagcgga tcaagagccg agaacgcttc gtccatcagc aagatctcag gatcatttgc
     3241 taaagctctg gctaggccaa cccgttgttg catcccacca gataactgac taggatattg
     3301 gtctttaaat gaaagcaaac tagaattttc taatgctttt tctgcttttt cttgtcgttc
     3361 ttctttaggt acaccacgga cttctaaccc gtattctgta ttttccaaga tcgttcgatg
     3421 tggaaacaga ccgaagtttt ggaaaaccat attgatttta tgacgacgga cttctcgcaa
     3481 ttcttctttg cttaacttcg atacatcttc tccatcaata tagatactgc ccgaagttgg
     3541 ctcaatcaga cgattcaata gacggatcaa tgtggatttc ccactcccgg aaagccccat
     3601 gatcacgaag atttctcctt cttttacgtc aaaattcacg tcatacacac caaccgttgc
     3661 accggttttc tttaaaatct ccgttttcgg ctggtgatca ttcatcatat ctaaagcggc
     3721 ttgtgtcttt ttaccaaata tctttgttaa atgttttact tctactttta ccaaacaaat
     3781 tctccttttt tatgtaaatt catttttctt tctgaaaaaa gtgaccacac tatgttaaca
     3841 tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg tccaaactga aaaaaacttg
     3901 gctaaatctt tgtaaataaa aggtttagcc aagttaatat ttttttaata tttcacacaa
     3961 tcattctgca agctgatgct ccgggtaaaa aaagtttgtc atacgctgta gtgtatattc
     4021 atttccaata aaatgaacga tgtcccctgc cgtaaattta gcataggggc caggtgaaac
     4081 aagcagttct tcattatgag aaattgcgat aactgtggcg ccagtcgcct gccagatatt
     4141 caactcgcta attgaacggt ccagatgcat tgctttttct gataaagata attcaaacgg
     4201 aagtaaagga ttcatcttat gtattcgctt cgtttgattg accagctgat ccaataattg
     4261 agaaaaatga ttcagctcat cttgttgctt ctccacactt gccatgattt ctgcttttgt
     4321 atcttggatg gattgtacat cttgatattg caataggaaa tcttttgctt tttccttcga
     4381 agcaacaaat gccccacttc catgccgtac ttccatgatt ccaagatcta ccagcacatt
     4441 gatagccttg cgggtagtct caggagaagc attgtacgta ctggcaagtg ttgagcgggc
     4501 atggatcttc tcacccactt tatatcggtt ttcaacgata cgttcggcta aatcaacagc
     4561 aatctgctgg tagcgcgggc ggacgatttt tttctttcca gacattttct ttcctctttc
     4621 tttttttact ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa ggggagaata
     4681 gcctcttgca acgactgatc atcgcttgat ctgttccttt tttactgggc aagctgtttg
     4741 acagtcatca caattcttac cggattttaa acgagtatag acaattgcgc ctgcacttcc
     4801 aaagattaac acacttaata agaatgtcgc catcctattc ctctccttct aatgacaata
     4861 tagtataaaa ctggttcttg gattttggct gacggaacaa aacataaacg agtcctatca
     4921 tcagtaaaat agcaagatac gtccctaacg ctacctgtcc accctccaat aagtgtccca
     4981 actgataaat cacaaaacta actaaataag ctaaaccgca ttgatagcct atggcgatcc
     5041 aagtccattt cttggcattc atttcccgat ggatagcccc aattgccgca aaacaaggcg
     5101 cacaaagtaa attgaaggct aacaatgaat aagctgctgc gggagtatat gtgtgttgta
     5161 gcacttgcca gatttctctg ccattttcgg atacttccgc atgaccaaac aaaataccaa
     5221 atgtgccaat aatattttct ttagcgacca atcctgtaat cgctgcgact gttccttgcc
     5281 agtttcccca accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa aaggctaaga
     5341 tactttggtc ttcagaaaca ggttgagcat aaaaattata agaagagctg aaccagatca
     5401 cgatactaga aacaaagata atcgttcctg cttttttaac aaaagacttg ctgcggtcat
     5461 aggtttgtct taggaccgct gaccattgag gaaaatgata tgcgggcaat tccatgataa
     5521 aaggagctgt atctccagca aacaaacgtg tttttttcaa gctgattcca gagaaaataa
     5581 ttgcagtcat accaagaaaa taggcagaag gagctaccca agattgatga ggaaaaaagg
     5641 cccctgctac taaggcaata atcggcaact ttgcagaaca aggcatgaag gttgtgacca
     5701 tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg actggccatg acgcctggca
     5761 ctccacatcc tgtagcaatc agcataggaa tgaaggattt tcctgaaaga ccgaatttcc
     5821 tgaaaagtcg atccatgaca aaagcgatcc tcgccatata tccgcagtct tccagaaagc
     5881 tcaagcataa aaacaatata atcaattgcg gtagaaatcc taacactgct cccacgcctg
     5941 cgatgatgcc gttcaagatc agctcctgca tccatcccgc tacttgccat tcagctaaca
     6001 atctgccaac atgttctggt aaccatgtac caaacaatgt gtcattgatc caatctgtcc
     6061 ccattgttcc tactgtttga atagccagat aatagatcag ccacatcaca aatgcgaaga
     6121 tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat tttgtcactc atcgataatt
     6181 ggaaagaatc atttgacaca acacagagtg tacatagttg ggtaataaat tcataacgtt
     6241 cattgaccag aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac tggatcagtt
     6301 gctcgatctc tttttgctga gaaactgcta tctcgtaatc ctctcttacc ttctgatctc
     6361 gttcaaaata tttcattgca taccagcgtt tcttgccgct ttgttctcct agtacatctt
     6421 caatttcatg taaagctgct tctaaacgtg gatcatatgt acagggaaca ggaatagaag
     6481 gccgcttttt tgcagactcc tgaatctttt cgattagttt ttcgattcct tgctttttca
     6541 atacgctgat tggtaaaact ggtacaccaa gaccatagga aagcttttct agattgattt
     6601 tttttccttc ttttttgatg acatccatca tattcagacc aatgatcata ggaatgtcta
     6661 aatccaataa ctgcgtagtt aaataaagat ttcgttctaa attggtagca tcaataatgt
     6721 tgatgatcag ttcaggctgt tcctgaacca aataatctct tgcaatcact tcttctggtg
     6781 tataaggaga caaagaatag atgccgggta ggtcctgaat atgaatgttc ggattttttt
     6841 tcaacgatcc ttcttttttt tctactgtta cgcccggcca attccctaca tattgattcg
     6901 aaccagtcaa gagattgaac aagctagttt tcccgctgtt tggatttccg actaacgcga
     6961 tattcattct ttttcctccc tttgtatcaa gattagttcg gcctctgact ttcgtaacgt
     7021 cagttcatac cctcgcactg tcagttcgat tggatctccc agaggggcta gttttcggat
     7081 aactattttt acatttcgcg tcaatcccat atccattaac cgtcgcttca ctgcgccttg
     7141 tccgtggatt gctacgacga tcccagtctc tcctgctttt aattgatcaa gagaagtcca
     7201 gctttcagag gaagcagtct ttttattgac aaagatttga cctagtattt cccgacttac
     7261 tgccactcga tgacttttta ccagtaaaat ggcattgttg cctgataaat tgacgacgac
     7321 gattttactt ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt tttcttctgt
     7381 ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg tctgcatatt
     7441 gattcctctc ttgtgaaata aatcatgtat taaaatcatt cattatatgt tctaattttc
     7501 ttacgtgtta cgcaaataaa tgagaatcat tctcacatag aatactctcg gtaattgaga
     7561 atgtcaatta tacttgtgat attatgagcg aatctttagt attgatagat aaaaatgaga
     7621 aaaaaatatt tcttcatagt tttccacaat tttttctcta ttaaatttgc tattttccca
     7681 cacgatactt gttttcagct cttttttcaa aaacacaaaa cttagtattc cacagtttcc
     7741 acaacacaat atgtagtgtt tgttgtttga cttttttact aagagtttta tactatctat
     7801 tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa tcttttctaa
     7861 aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc aaattgcttt
     7921 tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag cacaaggatt
     7981 ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc gtcctgatca
     8041 gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac atggatttga
     8101 agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt aaagcttaaa
     8161 aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt gggcgtacgt
     8221 atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga aatcaatcgg
     8281 ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc cttttttgag
     8341 gaaaatgtca aaccaaatac gatgactttt ctatctatta tggaaaaaat caattacttg
     8401 atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc atttattgaa
     8461 aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat ggctgcttac
     8521 aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct ggaaacttat
     8581 gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga attagcactt
     8641 gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc tttcttgaat
     8701 gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt cactgacgat
     8761 atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat tggtggcggc
     8821 gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg atatgagggt
     8881 gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag ctattctaat
     8941 caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca tccagacatt
     9001 attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt aaaaactctt
     9061 tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa cgaagatatg
     9121 tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc ttatgtaaat
     9181 attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca aaaaatcaaa
     9241 gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta tccttacgta
     9301 atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat catgagtaat
     9361 ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa acaggaatat
     9421 gaagtattag gaacggatat ctcatgtaac ttaggctcga caaatatcgt gaatttgatg
     9481 caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac atttattaca
     9541 gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct gaatcatacg
     9601 attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat ggaatatggc
     9661 tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta ttggacactt
     9721 gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt tgaaaaatca
     9781 gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca gccaaaatca
     9841 gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga ttggaatgct
     9901 ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc agtagcacca
     9961 aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat cactcgcatg
    10021 atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc ttatctatcc
    10081 aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa agtaatcgat
    10141 gtttatgcaa tggcacaaca acacgtcgat caaggcatga gtttgacttt attcatgcgt
    10201 tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac gacacgtgat
    10261 ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta ttacgtacgt
    10321 acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg cgtgatctaa
    10381 tatcatagat agattcgctt attcatattt taattaaagg agcattctca tgtcagaaac
    10441 atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat ccacttggga
    10501 gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg acctagatga
    10561 ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg gcggtttgac
    10621 actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag atgtccgcac
    10681 tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc atgccaaaag
    10741 ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa tttttgactg
    10801 gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa tctacaaaca
    10861 cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct tgttctattc
    10921 aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg tagcagagat
    10981 catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt acaaattcca
    11041 attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact ggatgtacaa
    11101 tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat acgatccaat
    11161 cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag cattgatgaa
    11221 cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa tcgtaatgaa
    11281 cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa acggctattt
    11341 gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg aataaggatt
    11401 tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga tattttaaat
    11461 gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt tggactgtct
    11521 aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat atttaatatt
    11581 atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat tcctgcgaca
    11641 cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat tgatttccca
    11701 aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat cgacaacgcc
    11761 atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg taccgctcca
    11821 ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc aagcaacaag
    11881 ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat aatcaaagca
    11941 tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt taccgtttgg
    12001 cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat aaatttgtgc
    12061 ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat aaaaaattgc
    12121 atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat gatattgaac
    12181 aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa atttttaaac
    12241 tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata cattggcaag
    12301 ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat gacagttaaa
    12361 acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct cgggatccga
    12421 tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc agcaacaagc
    12481 aatggtaaaa aaactgccga aagcattttc aatattggac taaaaatatt cggcatttgt
    12541 aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa aaataatttt
    12601 gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact tctattctat
    12661 catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca aaaaaaataa
    12721 acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt agtgacaaaa
    12781 attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat cttcgtaata
    12841 agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt attacttgat
    12901 gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact aaatgcaact
    12961 ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg agcccttttg
    13021 gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat tttacgtcaa
    13081 aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga aaatctttgg
    13141 tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca tggatcacgc
    13201 gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca atggaatagt
    13261 aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg gcagtcgtaa
    13321 gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa ccgacaccac
    13381 tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca agctgtggat
    13441 actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat gcaatgatac
    13501 atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt ttatattgtc
    13561 gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa ataatctctg
    13621 gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat catgtctgaa
    13681 aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg tgacaaaatt
    13741 tttgtcatag tcctttttat gaataaacgg tgttcaatca gctgaccctc ggtattactc
    13801 aacaaacggc aaaatatggg gagctatttt tcctttgtct tctctaatac tcaggtcaaa
    13861 acgctgattt cacaacctct ttagcctact attgaaaatt acaacttact tgctgcttct
    13921 tcatcgatga tcacaacaac attgtcgtga ttttgcaata cacttgctgg caaatgattc
    13981 gttacaggtc cttcaatcat ccctttgatt gcatcagctt tgctttcgcc aaatgccatc
    14041 aagacgattt ctttactttt cataattgaa ccgattccca ttgaaacagc ttttgttggt
    14101 acatcttctg ctttatcgaa gaatcgtttg tttgcattga ttgttgattc tgttaagtca
    14161 actactgaag tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat atgtccattt
    14221 tgcccgattc ctaagatttg gatatcgatt ggatgtgcat cgatgatttg atcatacttt
    14281 ttgcatgctg cttccagatc ttctgctttt ccatctggta caaacgtttc tttgaatggt
    14341 tttttatcaa ataattggac atccatgaaa tgacggtagc tttgttcatc tgaaccgcct
    14401 agtccaacgt actcatctaa gtttacagaa gtcatttcac tgaaatccaa atcgcttgaa
    14461 gtcatttctt tatataaagt aactggtgtg cttcctgttg ccaatcctaa tacttttgca
    14521 cctttgttca taccttcttt gatgatttca aatgcttttt ttccgccttc ttcagcgttt
    14581 tttacacgaa tgatttccat agtgattgct cctttatatt ttggtatagc ccaattatag
    14641 cattcttttt ttctttttac aataattagt atagaccaaa ttgcattttt ttcattttct
    14701 tgacaataaa taggaatccg tgttaggttt cataagatgg ttcaatcaaa caatccatgt
    14761 tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta tgtctgcagt ttttttatga
    14821 aaaaggagtt attatgagta tactgacatt aaacaacatt acccagcaat ttggcgacaa
    14881 aatcctctat gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg ggctgattgg
    14941 acaaaacgga gccggtaaaa gtacattgat taaaatcatc actggtgaga tcttaccaga
    15001 tgacggacag atccattggc aaaaaaatat tcacaaaggt tacctcgatc aatatgtgga
    15061 agtcgatgaa acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa aagaatacga
    15121 aaaggaagaa aaaattgctt ttctttacca aacatatagc gagaccttcg atgattcctt
    15181 gctggaaaaa gctgggaaac tccaaacgga attagaccaa ggagtgtttt atcaaatcga
    15241 caccttagtt gctgagatga gcagcgggtt ggggatcgat gtcttaggtt tagattcact
    15301 gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac tacttttaga
    15361 aaacccagat gttctgatct tagatgaacc aaccaatcac ttagatgatc agcatatcca
    15421 atggctgacg cagtttcttc aggattttga cggtgcgtat atcgtgattt cccatgacca
    15481 agaatttctt gatcagatca ccacccatat cgcagacatc gagtttggaa agattactaa
    15541 gtatactggc catctaaaac aagcattgaa acaaaaggaa caaaatcgcg aaagctattt
    15601 gcgccaatat catgctcaac aaaaacaaat cgaaaagacc gaagcttata ttcgaaaata
    15661 caaagccggt tcccgatcaa cgatggcaaa aagccgacaa aaacaattag ataaaatcga
    15721 acggctgacg cctccagctt cagcaagtaa agctgtcttt gattttccat tctcgcctat
    15781 tgtcacgaca ttagcagtgg aaacaactga tttagtcatc ggatatgatc gaccgctact
    15841 tgcacccatc aatttgacca tccgctttgg tgaaaatatt gccattcgag gcttcaacgg
    15901 gattggaaaa tctactttat taaaaacact gatcggtgag attgaaaaaa taagtggcga
    15961 tttccacttc ccagataaca caaaaatcaa ttacttttca caagatctgt actgggaaaa
    16021 tccattagaa acaccactac aatatttaag cagtcaattt ccaaaagcga ctatcaaaga
    16081 attacgtcgc caactagcta aagcagggtt agttaatcaa ttagcaagtg aacctctcac
    16141 tacattaagt ggtggggaac aaactaaggt gaaacttgca cagttgacaa tgaaccaagg
    16201 taatttactg attctcgatg aaccgacgaa ccatatcgat caagaaacga aagaaagctt
    16261 acaagacggg atacagaaat ttccaggaac agtaatcatc gtctctcatg aacaagaatt
    16321 ttatcaagat cttgtgcaac gagtcattga gattgaacca aaataaacgt ttgattgaat
    16381 agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac tccttcttct gaataaatgg
    16441 tggccaatta gctgaccttc ggtattagct caaaaaacgg caaaatatga ggagctattt
    16501 ttatttggct attcttatta cttgatgcag aacagctttt cacaacctct tttcgtctat
    16561 tttttatttt cgagatttct ctttccttta aaaacaactg tttgaaagaa gaacgcacat
    16621 ccttgaaaaa caagcccgct aaccgttaca cctaaccagc catactgctg ccacattgtc
    16681 gtccctagca atgaaccagc cgcacctcca ataaagtaca taaacataaa tacagtatta
    16741 tttcggttgc ttgctgcttc acctagcccc tgtactcgtg tttgattggc gacttgacca
    16801 aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa atggcttccg
    16861 ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata cacgattttt
    16921 ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc tccagacaaa
    16981 ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg actgctgata
    17041 taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa tccactgact
    17101 gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt aagtgaatca
    17161 aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca gtaaagcaga
    17221 agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca agaaaaaaga
    17281 ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa accgctaagc
    17341 aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc gtaaggaatg
    17401 atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc aaataacgta
    17461 aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctgc cattcgaatg
    17521 atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa ccctaacgca
    17581 tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac agataaacta
    17641 gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac aacgccacaa
    17701 gtgattgata atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt actcttcatt
    17761 gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa atagaaaaat
    17821 acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt cttgcgatct
    17881 ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa cgtgactgca
    17941 gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac agacgaaggg
    18001 atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat cgcatctgca
    18061 gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat tacacctatt
    18121 tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt tttcttatct
    18181 aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc attacccaaa
    18241 atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac tccaactact
    18301 gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa gagtatcgat
    18361 gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc tctcggattt
    18421 aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac taccagtatc
    18481 agaatgattc ccaacgagat caatatcaat cggattattt tctttttcat atttcctcct
    18541 gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag caccgaaatc
    18601 caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac agatgcgcca
    18661 acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc ttctttaact
    18721 ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc agttgcttca
    18781 cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc tacagtgaat
    18841 tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac aggagcagca
    18901 gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc taggatagtt
    18961 gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat ttcctccgtt
    19021 taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc catcttcttt
    19081 gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta gtacagatag
    19141 caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga tttcttccaa
    19201 tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg cttctttagc
    19261 aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg caacagctgt
    19321 aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg cacgtgaaag
    19381 aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt tacgtaaacg
    19441 tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg cagctgctgt
    19501 aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg cttcactcat
    19561 ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa aggcaagtca
    19621 cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc gttcatagtg
    19681 attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag tattgttcat
    19741 catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac cgcagacata
    19801 gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc ttgatgccac
    19861 ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc taaactagtt
    19921 gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat tagaaagaag
    19981 cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt ttcatgtatt
    20041 gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct gcaaagtttt
    20101 caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata tttccagctt
    20161 tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt gttacgtcca
    20221 ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat actcgtccta
    20281 gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag tcaaaccatc
    20341 cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca gctgcttcag
    20401 cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta ccagttccgt
    20461 ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct acgttcaaac
    20521 ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt gctaaagcta
    20581 ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca tcttgcattt
    20641 ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg ttgtacctcc
    20701 cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt agggtgtata
    20761 gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg cattgctgct
    20821 tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct tacttgatcg
    20881 ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc gattttagct
    20941 gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa tgaacggtct
    21001 tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt acgagcgttg
    21061 aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg acctactggt
    21121 ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc tactttcttt
    21181 gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca tatttctcct
    21241 cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca atgcacactg
    21301 gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt ttacattttg
    21361 cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc atttcttctt
    21421 ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa cacggttttt
    21481 tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg aaagatttaa
    21541 ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt agctctcaca
    21601 ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa cagcctgatt
    21661 ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac ggaacagata
    21721 ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta gctgattctt
    21781 tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt gaaaaagccg
    21841 aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt tcagtcactg
    21901 gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc aaaatttctt
    21961 taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt atgattgcta
    22021 aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca gtgacacggg
    22081 aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa gtagaagaaa
    22141 cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc gattaaggag
    22201 ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct tatccaagtg
    22261 tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt agtcacatca
    22321 tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag ggaaaagaag
    22381 gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat gattatttaa
    22441 atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat gctgtagctg
    22501 ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc ggaagcgcgg
    22561 gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca catgaacagc
    22621 aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac aacgcttcta
    22681 tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca atggcagcag
    22741 cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca gtagccatta
    22801 cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt gaagtcccgt
    22861 gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat atggcattag
    22921 cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac caagtcggac
    22981 gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt acgccaactg
    23041 ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc ttaatttcga
    23101 gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat tgcccttctc
    23161 ttcgtttttt attaatccat caatttcgta tattttactt catcgatcgt ttttgttcct
    23221 gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga tgttactcct
    23281 tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac taagtcagca
    23341 ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc gaaaatcact
    23401 ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga cgcaggacct
    23461 ccatctagtt gacgtccacc atgattggat acccagatac catctgcccc tgcagagata
    23521 gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag atgggtgatt
    23581 tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc ataaatttct
    23641 gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc agctaagtta
    23701 ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta accaccgagc
    23761 gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt gttcaaaatg
    23821 aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg tgcgcctggc
    23881 gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga aaagattgat
    23941 ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc caatccttgg
    24001 gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac agatgtatgc
    24061 aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt attaaaagaa
    24121 gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata gccaaaagct
    24181 cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac aatatcgatt
    24241 ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat cttcttgatt
    24301 acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca gatactttta
    24361 tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt tttcatgaag
    24421 catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat ttccgcgttg
    24481 cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat cactcaagtc
    24541 tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa tattaccgat
    24601 catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg ctaactcatc
    24661 tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa ttgctgttaa
    24721 tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc gtagtgcact
    24781 aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt tttctttcag
    24841 atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt tattatcttc
    24901 aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg ttgcttgtat
    24961 ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat ttttttccaa
    25021 acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga aaaaaagtgt
    25081 tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt tatcagaaac
    25141 ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac tgataaggac
    25201 taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca tacacctctt
    25261 cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga catttcaagt
    25321 ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg gtttcacgtc
    25381 caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct gttacttgtc
    25441 cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact tctacttcta
    25501 gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact tcttctggaa
    25561 gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc acaccaggtg
    25621 tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt acgtatcctg
    25681 ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt tctttttctt
    25741 caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca cgagattcga
    25801 tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac cagttttttt
    25861 caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct cagtacaccc
    25921 gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc gagttatttt
    25981 aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat caatccgaaa
    26041 ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct gcttggccaa
    26101 gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca acctcccttg
    26161 ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta caaaacttat
    26221 tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag ttgcgagacc
    26281 cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc ccccttgctg
    26341 agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca atttgtgaaa
    26401 taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt aagctaaaat
    26461 gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa aaaagatctg
    26521 acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga aacttccgga
    26581 taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa catttacaaa
    26641 atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt cactcatctt
    26701 ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt attctttacg
    26761 atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta tggtattttg
    26821 acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca aggaagctat
    26881 gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc aaaaatcatt
    26941 tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga cgaatacatt
    27001 cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga aacgatcgtc
    27061 aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca aaaccaagaa
    27121 gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc gctttttata
    27181 attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat ggtgctgaaa
    27241 cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa aatgcttggc
    27301 cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag aagatcgtgt
    27361 tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct gtcaggccta
    27421 aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa ccaaagaagg
    27481 cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta aagacaacta
    27541 aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca ggcgcaacta
    27601 aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt acggataacc
    27661 gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt ttttaacgcg
    27721 ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga cttttcattt
    27781 atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt aaacgtttgc
    27841 taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt cccagtaaag
    27901 cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa taaaaaagga
    27961 tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg aagatctaag
    28021 tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc gcttgctagg
    28081 cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct gctataataa
    28141 ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca ttcaaaaaat
    28201 agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat atcttgtttt
    28261 attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa tccatcggca
    28321 ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa aaataagcat
    28381 attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat tgctgaaact
    28441 ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat cgatactttc
    28501 ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga tactttctat
    28561 cagatggaag tagaattccc agttgcttat attccagcag gttctggaga tgattttgct
    28621 cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt agcagctcaa
    28681 tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa aaaaggaata
    28741 gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac aaatcattct
    28801 tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt attctctatt
    28861 ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg aggaaaacaa
    28921 ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt tggcggagga
    28981 atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt cgtggtcgaa
    29041 cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa aaaacaattg
    29101 aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc aacaacaccg
    29161 caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat cacaataacc
    29221 acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct acatctggaa
    29281 caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct ttttttacta
    29341 tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt acccaaaata
    29401 aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt gaaccaaaaa
    29461 gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt attttgaaca
    29521 aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa gtattctgct
    29581 ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt gatcccaccg
    29641 ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat gatcggcaat
    29701 aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga ggtgtggatc
    29761 actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta actaatcaaa
    29821 ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg gtttcgttta
    29881 ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa aatggataga
    29941 aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt catccgctga
    30001 ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg tgtctgttcc
    30061 atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg caaaaaggtg
    30121 ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg tatcttgatc
    30181 gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg taagataatg
    30241 tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc atccaatgta
    30301 acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg cgactcttct
    30361 tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc aatattttgc
    30421 gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt cgatttattt
    30481 gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc tttccatcca
    30541 agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat tggtataaaa
    30601 actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca agttgacgag
    30661 atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt gttccgaccc
    30721 catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc tatccttttg
    30781 caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa tcgcacacaa
    30841 cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt ctacttgata
    30901 gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg taaagcttgc
    30961 tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca agcgaatacc
    31021 atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca tcgtcccgta
    31081 gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca tattacgacc
    31141 tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg gcgcgagatc
    31201 aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta aaaattgatc
    31261 catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga gcgaaacaga
    31321 tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt cattcgcttc
    31381 tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga cttgttctaa
    31441 gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa ttccagtatt
    31501 aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa acatcaatcc
    31561 taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca gcagtatttt
    31621 tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct ctcaaatcct
    31681 tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct atttttccgt
    31741 aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta tttagttcag
    31801 tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct attgttcgct
    31861 gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc cgtccgtacc
    31921 atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc catgatcacg
    31981 atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac aatggttgct
    32041 ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg ggggtctaca
    32101 gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc acatgcgatg
    32161 ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac atatttttct
    32221 aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca
    32281 tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa gaccgcaacg
    32341 tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt cattgttgtc
    32401 ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca gttgatggca
    32461 gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc ttgtacttgc
    32521 agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt atcaatttcg
    32581 ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa taaagtgcaa
    32641 gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa tgaaggagtc
    32701 tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta tctcattgct
    32761 tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga tcgccgtttt
    32821 attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc aagttattct
    32881 atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga cgatgcgagt
    32941 gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt tgttgatttc
    33001 gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc
    33061 cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt acattgtctc
    33121 caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag cattggccct
    33181 gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga
    33241 catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga
    33301 aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg ccaccgttca
    33361 cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat cgatctcttt
    33421 tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga ttgaaggaaa
    33481 cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca tcaaagaagc
    33541 cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt
    33601 gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa aaaattctgc
    33661 tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca
    33721 tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg agggacacca
    33781 atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta caaaccattt
    33841 tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag gatgcgctag
    33901 tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg cctaaacaaa
    33961 cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc cttttgcttt
    34021 tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt gtcaaagggt
    34081 atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct tataatggac
    34141 aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag aataaaccga
    34201 cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc atcaaagaag
    34261 tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt gacggcactg
    34321 ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc acgcaattgg
    34381 ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa cctgaatgat
    34441 gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc gcaaaccatt
    34501 tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg gattgttcgg
    34561 cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg attcttcacg
    34621 atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca tctcatctaa
    34681 ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt cggtttcttt
    34741 agccataaag taacgccccc cttttgattt cagcttagca tatcacactg aaaaaacaaa
    34801 agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc aagcattttt
    34861 tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat ctactaactg
    34921 gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt ctacctcttc
    34981 tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt ccattacttg
    35041 gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt ttacaacccg
    35101 tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag aatcttcata
    35161 agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga tccttgattt
    35221 caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg cttgctcttc
    35281 ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca aattacgctg
    35341 aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt cactgccttg
    35401 tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt ttcttttata
    35461 agtgacctca tttgcatcaa actggataac taattcatcc actttatctt cagaatcttt
    35521 tgccaagatg atacccgtga tttctcccat atctccattg aatacattca attcgggagt
    35581 attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc aatgtacttc
    35641 cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta acgcatcgat
    35701 ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac ggaatccctt
    35761 atttttcgct ttttccacaa ttttagagat caagggttca atttgataag catcacttcg
    35821 gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc cttctttgat
    35881 ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct gattgagttc
    35941 cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag gaccgacaga
    36001 gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa tcgctttgaa
    36061 taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca aaagtccgcc
    36121 ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc ccagaagacg
    36181 atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg ctcggccagt
    36241 tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat ccaattccag
    36301 tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt tccccgtacc
    36361 tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag cttgttcttg
    36421 cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct tctgcacttc
    36481 tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg aattaccgat
    36541 cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt gttgtatctt
    36601 tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga tttcaattgg
    36661 gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg gtacgtatgt
    36721 atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc gaattcttct
    36781 cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt tgaacccgat
    36841 tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca aagactcgtt
    36901 cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac gattcagtcc
    36961 catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca tttctctttt
    37021 tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga ttttttcgat
    37081 agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga ttcctgggaa
    37141 tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt cttgctcata
    37201 tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc cataaaaacg
    37261 ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct ctttttcacg
    37321 gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat tactaggatt
    37381 ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg gctgcatcgg
    37441 aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct aaaaaatctg
    37501 tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca gtctctaaaa
    37561 tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc tcttttcctg
    37621 tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa agtgggccat
    37681 ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt gccgattcaa
    37741 tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc aaattcggca
    37801 gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa gtacccagcc
    37861 ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc tctttgttga
    37921 taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct ttaaaaggaa
    37981 catattttac ctttttccca aatgctttga tttcttcgta actttgtggc aaaagcggag
    38041 aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc ccatgtcggg
    38101 taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact ctataataaa
    38161 ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta gggaatcctt
    38221 gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc aatgagcgga
    38281 catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc ataaatgttt
    38341 ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca tcagcaaaat
    38401 tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta cgattaccta
    38461 aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca ttccaataat
    38521 ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc tcgattaaat
    38581 gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt gcagggatta
    38641 atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt atgttcgttt
    38701 ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg tgtcagtcac
    38761 tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca ggaacctgat
    38821 aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt ttttgcaacc
    38881 tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa taattctatg
    38941 tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg ctcttctacc
    39001 tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg tatctcaaat
    39061 tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt ggctactaca
    39121 cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg acgtagttcc
    39181 tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga gagtacacgt
    39241 tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa tgtttgatca
    39301 gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga ttgttctgta
    39361 gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat ctttttcaat
    39421 tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag cgagacttct
    39481 tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga ttggatcatt
    39541 ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg ggataaaaaa
    39601 taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat caagccatta
    39661 tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg attttctgcc
    39721 aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt ctgttttatc
    39781 ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc tagaagaaag
    39841 tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac tacacatcta
    39901 ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac ttagcggtcg
    39961 gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct ggggaaaaag
    40021 atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat aacgatatcg
    40081 aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg gatggcgtag
    40141 tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa ctattgatcg
    40201 aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt cctgaagatc
    40261 ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc ttgactcatg
    40321 gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa ttgattgcct
    40381 atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag aatgcagcac
    40441 atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg gatcttcagt
    40501 tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc aaatgttttt
    40561 gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa tcagtacact
    40621 cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat caaatcctag
    40681 acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga ttgcaaatag
    40741 catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac ctacgattag
    40801 aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt cagccaaaac
    40861 aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa cagccacagc
    40921 tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga agactgctaa
    40981 aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg tcgacatacc
    41041 taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta ctggattttc
    41101 attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag cttcatcatc
    41161 aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat cataatcggt
    41221 aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt cttggtcgtc
    41281 gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg cgtcgtcatc
    41341 attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt gattaacgac
    41401 atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt acatcgcaga
    41461 gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg cgccagccgt
    41521 ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg acatcaaact
    41581 taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag cattatctgt
    41641 atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta ggtattcttt
    41701 catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt taggcgacag
    41761 ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt gcccctgctc
    41821 atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt cccttaaatc
    41881 ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac caatctcacc
    41941 tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa aggctgcttg
    42001 aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca atgaagggct
    42061 tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag cagggcacag
    42121 ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag ctggatatca
    42181 actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc cgaacgtcct
    42241 tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg caaatcgata
    42301 tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata ttttttcaca
    42361 aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg ctttgcaaaa
    42421 agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac ttggttttcg
    42481 taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt tggatggtat
    42541 tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg ttcatcttca
    42601 tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc ttcagcagga
    42661 atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc tacttgatca
    42721 ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt tgagatccct
    42781 ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc ccatgtaaac
    42841 tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat gcatatgtcg
    42901 ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc ggatatttgc
    42961 ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt ccaattttat
    43021 ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa acactcgatt
    43081 attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga tcacagcacc
    43141 gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca atccatctcc
    43201 tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg tacgattggc
    43261 ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt catccaaata
    43321 actgatccga acatccagca agggataact aacactttgt tcccggttcg ttcgtaagct
    43381 gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta aatgacctgt
    43441 atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga acgccgacaa
    43501 taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat ctcgttgaat
    43561 ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct ttgcttcttt
    43621 attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct ttcttcaaaa
    43681 gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac cgctgtgctt
    43741 cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca gcttctgccg
    43801 cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta taattcagcg
    43861 aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt tccgccaaaa
    43921 ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga taagaacggt
    43981 agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc atgtcttttc
    44041 ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac atgatcctta
    44101 acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc cgtaattgtg
    44161 attttgttac aatactatcg actggcttga ctctcccagt gacaaattca atcgggacat
    44221 gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc aattctgata
    44281 cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct gatacctcta
    44341 cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt actcttgatt
    44401 tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc gatcaaatga
    44461 cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac gagatattca
    44521 tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt tatttcgata
    44581 ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc gttggtcatc
    44641 aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca acggatacct
    44701 ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt tcaggcgcag
    44761 tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa accggaaagc
    44821 ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt gatgaaagct
    44881 ggggagacaa tctacacgat tttgaactgg aactagaggt aaggggatgc atctgtggga
    44941 aaaattgctt ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac
    45001 aagatccaac gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg
    45061 agttcatttt tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt
    45121 tgtatagaat ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct
    45181 ttaggggaat actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac
    45241 gggacaccat caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca
    45301 attggggatg tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac
    45361 ttattctcaa tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag
    45421 aaaaaaggcc gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca
    45481 tattctcaag aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt
    45541 aaagaagatc gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct
    45601 cgggaaatgg gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt
    45661 gattttggtg ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg
    45721 tgtaaaacaa cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat
    45781 tctcagttcc attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg
    45841 actcccttac aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag
    45901 aaagccttat aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga
    45961 cattgcatct tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc
    46021 cacaggataa tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg
    46081 ttcctggttt agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg
    46141 gaaaacatag taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg
    46201 gtcttcttcg actgctggac cataatattt tgcattcaat ttaccataac tatcacttag
    46261 gtattcacta gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc
    46321 agcgaattgt gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac
    46381 ataagcccgt acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc
    46441 attcgttgtc gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg
    46501 gttcgaacga gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag
    46561 ctgatccagc gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga
    46621 agaataagca ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa
    46681 tgctttttct acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt
    46741 ttcttttgct tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag
    46801 atcatacatg tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg
    46861 taacaaaggc aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat
    46921 atgattattg ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa
    46981 attatgtcct tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta
    47041 aacactgtac aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa
    47101 ctgaccatac acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg
    47161 aaaaactaga tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc
    47221 acctgctcca gcaagaagag attcctgctc agcagataag acatgggctc gattatctac
    47281 tatttgctgg atataatggc gatagacttc caattttggt tcttctttga aatattgcca
    47341 aatctggtca tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac
    47401 tttcgaaaac agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc
    47461 ttgatcgttt ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa
    47521 tacgaattca atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt
    47581 ttcagattgt tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct
    47641 tgaaaagatc ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa
    47701 ctgctttact tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga
    47761 tcatagtcta acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct
    47821 tccatcaatt ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt
    47881 tttatactta ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat
    47941 agaccatctc ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc
    48001 aaagccatga gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc
    48061 gataaagaaa aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg
    48121 atgatagatg caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat
    48181 tcttttgctt gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt
    48241 ctattttttt catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc
    48301 tactagaaaa agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa
    48361 tatgatgata caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc
    48421 tttttctatt atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa
    48481 aagaattacc aagtatccac catacttggt aactgttatt caaataattt tgggttcttt
    48541 caataaatct ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca
    48601 aaatatccgg cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg
    48661 actgtcccag ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt
    48721 cactaaagac cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc
    48781 cctttttcaa aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg
    48841 catgtttagc aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc
    48901 tgaacttatt tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt
    48961 ctgcatcaag taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg
    49021 ggcttgttac cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac
    49081 aatttttgtc acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct
    49141 cgatagcatt acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat
    49201 cgctcttttc agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga
    49261 gattttccat ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag
    49321 tccaagcaga ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa
    49381 aagcaaaagt tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg
    49441 gatcgctgcc aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag
    49501 aatcttctga ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc
    49561 cgtctccttt tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga
    49621 tatcaacttc ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta
    49681 aatccaaaaa tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg
    49741 tattttcatt aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa
    49801 tatagattca ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc
    49861 gaatttaata aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat
    49921 ttttggcaga cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg
    49981 tgcaatcatc atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa
    50041 caaaaaaact tggaaaaact atctttgtaa tagtcttttg gctctatact ttttgatgtt
    50101 ggtgaacttc accaacatct tttttgta
//
LOCUS       DAZKMZ010000012        49852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000012 DAZKMZ010000000
VERSION     DAZKMZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.012"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8M5_000804"
     CDS             complement(105..629)
                     /locus_tag="ACG8M5_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0358078.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8M5_000805"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8M5_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0358079.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8M5_000806"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8M5_000806"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0358080.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8M5_000807"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8M5_000807"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0358081.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8M5_000808"
     CDS             3157..4026
                     /locus_tag="ACG8M5_000808"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0358082.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8M5_000809"
     CDS             4052..4567
                     /locus_tag="ACG8M5_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0358083.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8M5_000810"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8M5_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358084.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8M5_000811"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8M5_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358085.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8M5_000812"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8M5_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358086.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8M5_000813"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8M5_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0358087.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8M5_000814"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8M5_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0358088.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8M5_000815"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8M5_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0358089.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8M5_000816"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8M5_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358090.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8M5_000817"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8M5_000817"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0358091.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8M5_000818"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8M5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0358092.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8M5_000819"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8M5_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0358093.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13653..13973
                     /locus_tag="ACG8M5_000820"
     CDS             13653..13973
                     /locus_tag="ACG8M5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0358094.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14127..15032
                     /locus_tag="ACG8M5_000821"
     CDS             14127..15032
                     /locus_tag="ACG8M5_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358095.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            15029..15802
                     /locus_tag="ACG8M5_000822"
     CDS             15029..15802
                     /locus_tag="ACG8M5_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0358096.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15983..17407)
                     /locus_tag="ACG8M5_000823"
     CDS             complement(15983..17407)
                     /locus_tag="ACG8M5_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0358097.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17618..18064
                     /gene="nrdI"
                     /locus_tag="ACG8M5_000824"
     CDS             17618..18064
                     /gene="nrdI"
                     /locus_tag="ACG8M5_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0358098.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18129..20111)
                     /locus_tag="ACG8M5_000825"
     CDS             complement(18129..20111)
                     /locus_tag="ACG8M5_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0358099.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="ACG8M5_000826"
     CDS             complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="ACG8M5_000826"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0358100.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(21024..22085)
                     /locus_tag="ACG8M5_000827"
     CDS             complement(21024..22085)
                     /locus_tag="ACG8M5_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0358101.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22296..22823)
                     /locus_tag="ACG8M5_000828"
     CDS             complement(22296..22823)
                     /locus_tag="ACG8M5_000828"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0358102.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="ACG8M5_000829"
     CDS             complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="ACG8M5_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0358103.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24650..25771)
                     /locus_tag="ACG8M5_000830"
     CDS             complement(24650..25771)
                     /locus_tag="ACG8M5_000830"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0358104.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25897..26838
                     /locus_tag="ACG8M5_000831"
     CDS             25897..26838
                     /locus_tag="ACG8M5_000831"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0358105.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26988..27884)
                     /locus_tag="ACG8M5_000832"
     CDS             complement(26988..27884)
                     /locus_tag="ACG8M5_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0358106.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            28153..29493
                     /locus_tag="ACG8M5_000833"
     CDS             28153..29493
                     /locus_tag="ACG8M5_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0358107.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29711..30154
                     /locus_tag="ACG8M5_000834"
     CDS             29711..30154
                     /locus_tag="ACG8M5_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0358108.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30658..32076)
                     /gene="nhaC"
                     /locus_tag="ACG8M5_000835"
     CDS             complement(30658..32076)
                     /gene="nhaC"
                     /locus_tag="ACG8M5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0358109.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32479..33702)
                     /locus_tag="ACG8M5_000836"
     CDS             complement(32479..33702)
                     /locus_tag="ACG8M5_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0358110.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33733..34572)
                     /locus_tag="ACG8M5_000837"
     CDS             complement(33733..34572)
                     /locus_tag="ACG8M5_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0358111.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34589..35269)
                     /locus_tag="ACG8M5_000838"
     CDS             complement(34589..35269)
                     /locus_tag="ACG8M5_000838"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0358112.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(35271..36344)
                     /locus_tag="ACG8M5_000839"
     CDS             complement(35271..36344)
                     /locus_tag="ACG8M5_000839"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358113.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(36400..36638)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(36681..36914)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(36991..37662)
                     /locus_tag="ACG8M5_000840"
     CDS             complement(36991..37662)
                     /locus_tag="ACG8M5_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0358114.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(37749..40397)
                     /gene="mgtA"
                     /locus_tag="ACG8M5_000841"
     CDS             complement(37749..40397)
                     /gene="mgtA"
                     /locus_tag="ACG8M5_000841"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0358115.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(40739..40913)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            41086..41523
                     /gene="ndk"
                     /locus_tag="ACG8M5_000842"
     CDS             41086..41523
                     /gene="ndk"
                     /locus_tag="ACG8M5_000842"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0358116.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(41545..42411)
                     /locus_tag="ACG8M5_000843"
     CDS             complement(41545..42411)
                     /locus_tag="ACG8M5_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0358117.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(42571..44664)
                     /locus_tag="ACG8M5_000844"
     CDS             complement(42571..44664)
                     /locus_tag="ACG8M5_000844"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0358118.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(45413..46036)
                     /locus_tag="ACG8M5_000845"
     CDS             complement(45413..46036)
                     /locus_tag="ACG8M5_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358119.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(46195..47592)
                     /locus_tag="ACG8M5_000846"
     CDS             complement(46195..47592)
                     /locus_tag="ACG8M5_000846"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0358120.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(47624..48595)
                     /locus_tag="ACG8M5_000847"
     CDS             complement(47624..48595)
                     /locus_tag="ACG8M5_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358121.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            48969..49394
                     /locus_tag="ACG8M5_000848"
     CDS             48969..49394
                     /locus_tag="ACG8M5_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358122.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        14360 a        10386 c         8798 g        16308 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg
    13321 taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg tctagttact
    13381 gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc
    13441 ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga
    13501 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg
    13561 taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt
    13621 gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag
    13681 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
    13741 attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa
    13801 tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt
    13861 cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
    13921 tggggatgct actcatcagt atcattggca tcaagattac tagcggccac tagacacttg
    13981 gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa
    14041 aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac
    14101 tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga
    14161 actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg
    14221 ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac
    14281 tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc
    14341 ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa
    14401 caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta
    14461 tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg
    14521 gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt
    14581 tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta
    14641 ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg
    14701 aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa
    14761 tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag
    14821 ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc
    14881 ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata
    14941 tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc
    15001 tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa
    15061 attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg
    15121 aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca
    15181 gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat
    15241 ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg
    15301 agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt
    15361 agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga
    15421 gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta
    15481 tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc
    15541 cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat
    15601 ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa
    15661 tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa
    15721 tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt
    15781 atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag
    15841 ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa
    15901 catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc
    15961 tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg
    16021 caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta
    16081 caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa
    16141 caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac
    16201 ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt
    16261 ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa
    16321 agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg
    16381 tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca
    16441 aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc
    16501 aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa
    16561 ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca
    16621 tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata
    16681 tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag
    16741 aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac
    16801 tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa
    16861 tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta
    16921 atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta
    16981 gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac
    17041 cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt
    17101 tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa
    17161 aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat
    17221 tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag
    17281 ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg
    17341 ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg
    17401 tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt
    17461 tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg gcttttttct
    17521 cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa
    17581 gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat atatatcgat
    17641 ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc
    17701 agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata gtgattttac
    17761 gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg gaaatggttt
    17821 agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg aacatgaaga
    17881 caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca ataatcagta
    17941 ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg cagattatga
    18001 attacgggga acaccatctg atgtgacacg aatttatcag attttagcag aaaacaataa
    18061 ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt tctattgttt
    18121 ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt acctgttttt
    18181 tccaagacaa agttttttac acgatccata ttggttacca agacgatcat ggatgtttct
    18241 ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc aggtgtcact
    18301 tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt
    18361 cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt
    18421 gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt ttctggttct
    18481 acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc tttatctaaa
    18541 gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc tgaagtttga
    18601 ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa ttcttctttt
    18661 tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc agcataagct
    18721 gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg
    18781 gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat cactgccccg
    18841 ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa tgttactccg
    18901 taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt gatcatttct
    18961 gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc cacttgtcct
    19021 gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc aatcatttgc
    19081 acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg
    19141 cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat cccaccgacc
    19201 atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat cagaccgatg
    19261 gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac tactttatgc
    19321 agttttttct caacgattga caatagccaa acagcgaaga tgacacctag gatcccacct
    19381 tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc cggattcatc
    19441 cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc accaaaaact
    19501 tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt tttcaagatg
    19561 ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag
    19621 tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc aggaatcatt
    19681 ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg tttttgtttc
    19741 gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt tccagatgct
    19801 gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt cacgcctgcc
    19861 aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat gacttgaagc
    19921 tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga gacatttaca
    19981 agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat gctttctaca
    20041 ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa agtcagttcg
    20101 ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt ttgcttcagc
    20161 taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga tagccagctt
    20221 aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc
    20281 ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt tgacatcgac
    20341 catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa tcatattcaa
    20401 gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa attctggacc
    20461 tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt tattgcaaga
    20521 aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga tcacatatgg
    20581 tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat tcagcttgat
    20641 tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta cagtcattgc
    20701 ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga atgtcggtac
    20761 gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca tataaatcag
    20821 acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga tacttgtcag
    20881 ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt gaacggcttg
    20941 agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt
    21001 ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg atagttcata
    21061 ccagctttga tttggttgat caatggtagc tcttctttga taactttagc tactacattc
    21121 actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat atagcgcaaa
    21181 tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt tcttgctggg
    21241 ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct tgcatcgtct
    21301 tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac aggttccgta
    21361 tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac gttttctgac
    21421 agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc tagagcgcct
    21481 gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata tagtgggcct
    21541 ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat tttgagccat
    21601 tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg ataataatta
    21661 tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc gatatcttta
    21721 ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc ggcaatctgc
    21781 tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt caaaggcgtt
    21841 agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat
    21901 tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg atatgccgaa
    21961 acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt ggatgccatt
    22021 tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat tgaaaagcca
    22081 aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag tatatatcta
    22141 aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta aaggttgagt
    22201 ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc
    22261 ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta
    22321 ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc
    22381 actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat
    22441 tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat
    22501 cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca
    22561 taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga
    22621 tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg
    22681 attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt
    22741 tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct
    22801 ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc
    22861 ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta
    22921 gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc
    22981 aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc
    23041 catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg
    23101 cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg
    23161 aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt
    23221 cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat
    23281 ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt
    23341 cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt
    23401 ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta
    23461 tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt
    23521 ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc
    23581 gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg
    23641 aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca
    23701 gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg agccatttca
    23761 aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc
    23821 agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca
    23881 tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta
    23941 ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca
    24001 aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc
    24061 cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca
    24121 aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc
    24181 gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt
    24241 agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat
    24301 tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg
    24361 aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag
    24421 tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat
    24481 acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg
    24541 tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact
    24601 ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga
    24661 ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc
    24721 tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga
    24781 gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt
    24841 ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct
    24901 gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc
    24961 cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg
    25021 ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga
    25081 aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat
    25141 ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat
    25201 tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa
    25261 aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac
    25321 ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa
    25381 taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg
    25441 aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg
    25501 cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc
    25561 gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat
    25621 agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt
    25681 tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt
    25741 taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag
    25801 acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac
    25861 taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc
    25921 ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg
    25981 atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt
    26041 aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc
    26101 aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt
    26161 tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta
    26221 ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg
    26281 atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc
    26341 gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt
    26401 caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa
    26461 gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa
    26521 ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct
    26581 atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct
    26641 acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa
    26701 aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa
    26761 tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga
    26821 atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct
    26881 ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa
    26941 ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca
    27001 acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt
    27061 tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt
    27121 gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca
    27181 ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa
    27241 tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga
    27301 atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt
    27361 tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc
    27421 tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc
    27481 gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc
    27541 agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca
    27601 tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac
    27661 acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa
    27721 tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc
    27781 gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa
    27841 agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt
    27901 tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc
    27961 ctatatttgt atgaaaattt aacatttgta tgaaaatatg atgaaataaa tcattcttct
    28021 attaaaattc gcctaaaagt tttctgaata gttgtaattg tctgaaaatt tgttatcatt
    28081 agtcattgtt aaatattgct atttattctt aatataaatt ttgattgtga atttaaaaga
    28141 aagaggtcat ttatggaaga taaaaaattg aaaaaacaac ttagctccag acatatcacc
    28201 atgttagctt taggtggagc aatcggtgca ggtctattta aaggaagtgg cgaagcgatt
    28261 gggatcgccg gcccttctgt attgattgct tttttgattg gtgggctgat tttatatatc
    28321 gtgatgaaag gtctgggaaa aatcgtttta agcggtggcg acacgcatca cggattatct
    28381 ggtttgatac gcccttatct aggtgctcat tctgctgatt ttactgattg ggtctactgg
    28441 tctatgtgga ttatcaatat catcgctgaa gctgttgcag ctgcttcctt tttgcagttg
    28501 tggttcccgc acattccagc ctgggtattt gtttttatgt tggctgtttt aactacgatc
    28561 atcaatcttt attcggtacg gttattcgca gaaacagaat attggctggc ttttgctaaa
    28621 atcagtgtca ttatcttact gatcattttt gctacttatc ttgtcggtca gcaaatgttg
    28681 ggcacaggcg ttttccctac ccttcagcaa ttgactgacc atggtgggtt tactcctcat
    28741 ggtatgaagg gaatcatcag ttctttgctt gtagttatct attcttacgg cggttctgaa
    28801 ctgattgcta tcacggtaag cgaagcagat gatcctaaac gtgcgattcc taaagcaatc
    28861 aaaggtgtca tgggaagaat catttccttt tacatcattc ctttgttttt gcttttgatt
    28921 ctttttcctt ggaatacttt agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa
    28981 aaaatgaata tcccatttgc agcggatatc gtcaattttg tgattgtcct tgctttgttc
    29041 tcatctatca attcgggtgt gtatgcatct tcaagaattt tgttcttccg tttgaaggat
    29101 cgaaaaggtt cctctagaaa actagctgtt ttaaacaagc atcaagtacc gcaacgtgct
    29161 gttttatttt gtaccagtgt tctttatctt ggtgtagctc tttcttattt tgtcggtgac
    29221 aaattatttg gctatctggc aggttccctt tcgtatacag tattattgat ctggatcatt
    29281 atcagtgcgg catcctttgt actggcacta agaaaaggaa cattatggga taaaggaatc
    29341 agcttttttg cattagctgt cttaggattg attttccttg gtatcctgtt cacgaactcc
    29401 attggggtaa ctgtattgac tggcctgctt tacctgttca tttatttcag ttatcaaaaa
    29461 aagaatgatg cgttcgtatt gacgaatgaa tgaaatcgat agaaaatttt attttattta
    29521 tgtggaatag atggagctat tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct
    29581 ttttccgaat aaacagtctc atgacctctt ggtaaacggt gttcaaaaac cctctgtgca
    29641 aaataattga ttgttctcca tgctcatgac atgatagaag aaaggaatga aaaaaaggag
    29701 tttgttttaa atgaaaaagt attggaagtt tttgttgatg atcggtgttt caacggtagt
    29761 tatgtttggt atgatgtact tgaatgtcta tgcgctcgat catatcttct tcagtcgtac
    29821 acgagtattc atggcgttga tgatgggtgc agtcatggca gtcatcatgc tgttgttcat
    29881 gtggaaaatg tatgaaaaca aaaagataaa tgttatgatc ttagccgcta gtcttcttgt
    29941 ttttgccggt tccttgttta tggtacgaag ccaaactact gtcaacgaca cagcttggat
    30001 gaaggccatg attcctcatc attctatcgc tatcctggca agtaaacgag ctgatttaaa
    30061 agatcctgaa gtaaaaaaat tagcggaaga aatcattcag gcacaagaag aagagatcga
    30121 caaaatgaaa aaaatgatca aagaaatgga atgaccatta tccgtccaaa taaaaaaaga
    30181 ggatgttcca gaagttatca gcttcaagaa ataagaagaa gctacttctg gaacgccgtg
    30241 aataaggggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
    30301 gcttctccta tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg
    30361 tttattaaga ggttgtaaaa tcagcgttct gacctgagta ttagagagac aaatggaaaa
    30421 atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg attgaacacc
    30481 gtttatcgag aggttgtgaa aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa
    30541 atggctttcc acatttttgt ataaatttgg cttaataccg agaaactggc ttttgaatac
    30601 cgtttattcg taaaagggat tgtgacaaaa gtcttgtcac aatccctttt tatgttttta
    30661 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
    30721 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
    30781 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
    30841 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
    30901 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
    30961 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
    31021 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
    31081 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
    31141 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
    31201 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
    31261 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
    31321 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
    31381 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
    31441 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
    31501 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
    31561 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
    31621 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
    31681 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
    31741 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
    31801 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
    31861 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
    31921 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
    31981 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
    32041 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
    32101 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
    32161 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
    32221 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
    32281 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
    32341 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
    32401 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
    32461 acacaagatt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
    32521 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
    32581 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
    32641 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
    32701 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
    32761 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
    32821 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
    32881 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
    32941 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
    33001 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
    33061 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
    33121 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
    33181 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
    33241 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
    33301 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
    33361 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
    33421 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
    33481 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
    33541 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
    33601 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
    33661 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
    33721 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
    33781 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
    33841 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
    33901 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
    33961 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
    34021 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
    34081 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
    34141 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
    34201 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
    34261 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
    34321 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
    34381 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
    34441 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
    34501 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
    34561 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
    34621 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
    34681 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
    34741 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
    34801 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
    34861 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
    34921 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
    34981 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
    35041 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
    35101 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
    35161 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
    35221 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
    35281 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
    35341 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
    35401 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
    35461 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
    35521 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
    35581 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
    35641 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
    35701 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
    35761 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
    35821 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
    35881 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
    35941 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
    36001 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
    36061 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
    36121 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
    36181 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
    36241 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
    36301 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
    36361 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
    36421 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
    36481 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
    36541 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
    36601 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
    36661 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
    36721 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
    36781 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
    36841 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
    36901 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
    36961 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
    37021 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
    37081 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
    37141 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
    37201 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
    37261 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
    37321 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
    37381 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
    37441 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
    37501 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
    37561 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
    37621 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
    37681 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
    37741 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
    37801 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
    37861 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
    37921 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
    37981 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
    38041 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
    38101 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
    38161 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
    38221 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
    38281 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
    38341 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
    38401 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
    38461 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
    38521 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
    38581 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
    38641 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
    38701 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
    38761 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
    38821 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
    38881 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
    38941 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
    39001 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
    39061 ttcagcattt cttcaatcgc accttttgtg atcatttgtg ttttaccagc tggatcagca
    39121 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
    39181 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
    39241 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
    39301 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
    39361 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
    39421 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
    39481 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
    39541 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
    39601 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
    39661 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
    39721 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
    39781 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
    39841 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
    39901 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
    39961 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
    40021 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
    40081 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
    40141 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
    40201 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
    40261 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
    40321 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
    40381 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
    40441 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
    40501 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
    40561 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
    40621 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    40681 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    40741 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    40801 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    40861 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    40921 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    40981 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    41041 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    41101 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    41161 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    41221 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    41281 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    41341 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    41401 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    41461 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    41521 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    41581 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    41641 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    41701 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    41761 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    41821 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    41881 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    41941 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    42001 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    42061 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    42121 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    42181 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    42241 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    42301 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    42361 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    42421 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    42481 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    42541 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    42601 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    42661 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    42721 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    42781 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    42841 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    42901 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    42961 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    43021 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    43081 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    43141 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    43201 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    43261 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    43321 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    43381 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    43441 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    43501 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    43561 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    43621 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    43681 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    43741 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    43801 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    43861 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    43921 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    43981 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    44041 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    44101 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    44161 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    44221 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    44281 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    44341 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    44401 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    44461 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    44521 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    44581 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    44641 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    44701 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    44761 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    44821 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    44881 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    44941 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    45001 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    45061 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    45121 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    45181 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    45241 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    45301 caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
    45361 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc
    45421 cataggattg atattgtcct tgtgatgact cattcgtaat aattgaggct tgattttctt
    45481 cacttcccgt tatcgtttgt ccactgccag aatctgaagg acttgttcct gtcgactcgt
    45541 ttttttgaga agagacagtt gttttcttga cttgttcatt tgtctttttc cattccgcta
    45601 aacgatcggc gaccactttt ttgtctactt tttcagaata agcggtgatc acttttccat
    45661 ttttcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
    45721 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
    45781 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
    45841 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
    45901 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
    45961 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
    46021 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
    46081 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
    46141 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
    46201 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
    46261 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
    46321 ccacgaccag ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg
    46381 aatggcttga tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg
    46441 gatcatgcca tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga
    46501 aataaagcag tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc
    46561 ttgttcatag atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt
    46621 gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt
    46681 tacccggcgt ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac
    46741 cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa
    46801 ttcacttgga aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc
    46861 tgctatttgc gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt
    46921 cagattcaag atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc
    46981 taacgctgaa gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc
    47041 ttctggataa tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg
    47101 agtaaaccat ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc
    47161 ataggcttca accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat
    47221 atcgaaatga tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata
    47281 acgatgatag aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg
    47341 gctccattgg atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc
    47401 ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt
    47461 ttgcggttct tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat
    47521 ccttccttct gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg
    47581 atattttttc atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta
    47641 tgatattttc catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta
    47701 acagctgttc tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg
    47761 gaaggatact cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc
    47821 cgattctgac accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc
    47881 tgacaaaaat agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt
    47941 ttctagaata gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt
    48001 ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc
    48061 cgattttttt atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat
    48121 cattcactaa tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg
    48181 gaatactcat ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa
    48241 tcagcccatc gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct
    48301 gatcggcaaa cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc
    48361 ctgagacaac ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga
    48421 agcttcgatt attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa
    48481 tcgcttctgc tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg
    48541 aaactgttcc cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt
    48601 ctcactcctg ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat
    48661 tctttgcata gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac
    48721 ttcctctcac tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc
    48781 atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg
    48841 cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc
    48901 ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg
    48961 gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga
    49021 ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg
    49081 tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag
    49141 atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca
    49201 cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag
    49261 atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag
    49321 aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca
    49381 tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta
    49441 tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat
    49501 tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc
    49561 atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct
    49621 gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc
    49681 tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa
    49741 acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg
    49801 aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa tt
//
LOCUS       DAZKMZ010000013        45669 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000013 DAZKMZ010000000
VERSION     DAZKMZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.013"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG8M5_000849"
     CDS             6..245
                     /locus_tag="ACG8M5_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358123.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG8M5_000850"
     CDS             312..521
                     /locus_tag="ACG8M5_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358124.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG8M5_000851"
     CDS             581..1252
                     /locus_tag="ACG8M5_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0358125.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG8M5_000852"
     CDS             1305..3281
                     /locus_tag="ACG8M5_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0358126.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG8M5_000853"
     CDS             3294..4049
                     /locus_tag="ACG8M5_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0358127.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG8M5_000854"
     CDS             4046..4870
                     /locus_tag="ACG8M5_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0358128.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG8M5_000855"
     CDS             4880..5128
                     /locus_tag="ACG8M5_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358129.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG8M5_000856"
     CDS             5171..7270
                     /locus_tag="ACG8M5_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0358130.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG8M5_000857"
     CDS             complement(7308..9365)
                     /locus_tag="ACG8M5_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0358131.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG8M5_000858"
     CDS             complement(9479..9691)
                     /locus_tag="ACG8M5_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358132.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG8M5_000859"
     CDS             9878..10345
                     /locus_tag="ACG8M5_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358133.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG8M5_000860"
     CDS             10342..10584
                     /locus_tag="ACG8M5_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0358134.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG8M5_000861"
     CDS             10602..10904
                     /locus_tag="ACG8M5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358135.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG8M5_000862"
     CDS             10976..13030
                     /locus_tag="ACG8M5_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358136.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG8M5_000863"
     CDS             13030..15642
                     /locus_tag="ACG8M5_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0358137.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG8M5_000864"
                     /pseudo
     CDS             15639..18170
                     /locus_tag="ACG8M5_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            18222..18554
                     /locus_tag="ACG8M5_000865"
     CDS             18222..18554
                     /locus_tag="ACG8M5_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358138.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG8M5_000866"
     CDS             18555..19148
                     /locus_tag="ACG8M5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358139.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG8M5_000867"
     CDS             19311..21005
                     /locus_tag="ACG8M5_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0358140.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8M5_000868"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG8M5_000868"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0358141.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG8M5_000869"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG8M5_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG8M5_000870"
     CDS             24078..25283
                     /locus_tag="ACG8M5_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0358142.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG8M5_000871"
     CDS             25299..25943
                     /locus_tag="ACG8M5_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358143.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG8M5_000872"
     CDS             25954..26760
                     /locus_tag="ACG8M5_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358144.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG8M5_000873"
     CDS             26782..27012
                     /locus_tag="ACG8M5_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358145.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG8M5_000874"
     CDS             27558..27833
                     /locus_tag="ACG8M5_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358146.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG8M5_000875"
     CDS             27875..28297
                     /locus_tag="ACG8M5_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358147.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8M5_000876"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG8M5_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0358148.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG8M5_000877"
     CDS             29920..30255
                     /locus_tag="ACG8M5_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358149.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG8M5_000878"
     CDS             30402..30596
                     /locus_tag="ACG8M5_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358150.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG8M5_000879"
     CDS             30628..33000
                     /locus_tag="ACG8M5_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0358151.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG8M5_000880"
     CDS             33061..34521
                     /locus_tag="ACG8M5_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0358152.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG8M5_000881"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG8M5_000881"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0358153.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG8M5_000882"
     CDS             36752..36901
                     /locus_tag="ACG8M5_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358154.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG8M5_000883"
     CDS             36975..37568
                     /locus_tag="ACG8M5_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0358155.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG8M5_000884"
     CDS             37584..38483
                     /locus_tag="ACG8M5_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0358156.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8M5_000885"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG8M5_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0358157.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG8M5_000886"
     CDS             39337..39597
                     /locus_tag="ACG8M5_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358158.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG8M5_000887"
     CDS             40038..40244
                     /locus_tag="ACG8M5_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0358159.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG8M5_000888"
     CDS             40244..40495
                     /locus_tag="ACG8M5_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358160.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG8M5_000889"
     CDS             40510..40947
                     /locus_tag="ACG8M5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358161.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG8M5_000890"
     CDS             40940..41647
                     /locus_tag="ACG8M5_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358162.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG8M5_000891"
     CDS             41812..42012
                     /locus_tag="ACG8M5_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358163.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG8M5_000892"
     CDS             42028..42207
                     /locus_tag="ACG8M5_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358164.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG8M5_000893"
     CDS             42548..42811
                     /locus_tag="ACG8M5_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0358165.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG8M5_000894"
     CDS             42805..43155
                     /locus_tag="ACG8M5_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0358166.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG8M5_000895"
     CDS             complement(43179..43793)
                     /locus_tag="ACG8M5_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0358167.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="ACG8M5_000896"
     CDS             44107..44529
                     /locus_tag="ACG8M5_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358168.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="ACG8M5_000897"
     CDS             44539..44742
                     /locus_tag="ACG8M5_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358169.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="ACG8M5_000898"
     CDS             44953..45537
                     /locus_tag="ACG8M5_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0358170.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16742 a         7205 c         9260 g        12462 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 catgaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatatta
//
LOCUS       DAZKMZ010000014        37151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000014 DAZKMZ010000000
VERSION     DAZKMZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.014"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2301)
                     /locus_tag="ACG8M5_000899"
     CDS             complement(148..2301)
                     /locus_tag="ACG8M5_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0358171.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="ACG8M5_000900"
     CDS             complement(2595..3443)
                     /locus_tag="ACG8M5_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0358172.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG8M5_000901"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="ACG8M5_000901"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0358173.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="ACG8M5_000902"
     CDS             complement(5944..6123)
                     /locus_tag="ACG8M5_000902"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0358174.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /locus_tag="ACG8M5_000903"
     CDS             complement(6170..7993)
                     /locus_tag="ACG8M5_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0358175.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /gene="helD"
                     /locus_tag="ACG8M5_000904"
     CDS             8271..10550
                     /gene="helD"
                     /locus_tag="ACG8M5_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0358176.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="ACG8M5_000905"
     CDS             complement(10643..11290)
                     /locus_tag="ACG8M5_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358177.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="ACG8M5_000906"
     CDS             complement(11469..12188)
                     /locus_tag="ACG8M5_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358178.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="ACG8M5_000907"
     CDS             complement(12319..13470)
                     /locus_tag="ACG8M5_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0358179.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="ACG8M5_000908"
     CDS             complement(13687..14427)
                     /locus_tag="ACG8M5_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358180.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG8M5_000909"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="ACG8M5_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0358181.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG8M5_000910"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="ACG8M5_000910"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0358182.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="ACG8M5_000911"
     CDS             complement(15388..16038)
                     /locus_tag="ACG8M5_000911"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0358183.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG8M5_000912"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="ACG8M5_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0358184.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG8M5_000913"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="ACG8M5_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0358185.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="ACG8M5_000914"
     CDS             complement(17481..18008)
                     /locus_tag="ACG8M5_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0358186.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="ACG8M5_000915"
     CDS             complement(18188..18973)
                     /locus_tag="ACG8M5_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0358187.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG8M5_000916"
     CDS             complement(18966..19835)
                     /gene="accD"
                     /locus_tag="ACG8M5_000916"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0358188.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="ACG8M5_000917"
     CDS             complement(19836..21215)
                     /locus_tag="ACG8M5_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0358189.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG8M5_000918"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="ACG8M5_000918"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0358190.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG8M5_000919"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="ACG8M5_000919"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0358191.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG8M5_000920"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="ACG8M5_000920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0358192.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG8M5_000921"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="ACG8M5_000921"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0358193.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG8M5_000922"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="ACG8M5_000922"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0358194.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="ACG8M5_000923"
     CDS             complement(25173..25397)
                     /locus_tag="ACG8M5_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0358195.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="ACG8M5_000924"
     CDS             complement(25450..26412)
                     /locus_tag="ACG8M5_000924"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0358196.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="ACG8M5_000925"
     CDS             complement(26422..26862)
                     /locus_tag="ACG8M5_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0358197.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="ACG8M5_000926"
     CDS             27168..28133
                     /locus_tag="ACG8M5_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0358198.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="ACG8M5_000927"
     CDS             complement(28190..28420)
                     /locus_tag="ACG8M5_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0358199.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="ACG8M5_000928"
     CDS             complement(28506..29393)
                     /locus_tag="ACG8M5_000928"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0358200.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="ACG8M5_000929"
     CDS             complement(29482..30705)
                     /locus_tag="ACG8M5_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0358201.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31768)
                     /locus_tag="ACG8M5_000930"
     CDS             complement(30722..31768)
                     /locus_tag="ACG8M5_000930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0358202.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="ACG8M5_000931"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="ACG8M5_000931"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0358203.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="ACG8M5_000932"
     CDS             complement(33659..33925)
                     /locus_tag="ACG8M5_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0358204.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="ACG8M5_000933"
     CDS             34404..36638
                     /locus_tag="ACG8M5_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0358205.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="ACG8M5_000934"
     CDS             36786..37106
                     /locus_tag="ACG8M5_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0358206.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10450 a         8038 c         6479 g        12184 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcgtatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatagtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tcaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa a
//
LOCUS       DAZKMZ010000015        35500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000015 DAZKMZ010000000
VERSION     DAZKMZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.015"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..381)
                     /locus_tag="ACG8M5_000935"
     CDS             complement(<1..381)
                     /locus_tag="ACG8M5_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358207.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQK"
     gene            complement(566..964)
                     /locus_tag="ACG8M5_000936"
     CDS             complement(566..964)
                     /locus_tag="ACG8M5_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0358208.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(961..1734)
                     /locus_tag="ACG8M5_000937"
     CDS             complement(961..1734)
                     /locus_tag="ACG8M5_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0358209.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1731..2414)
                     /locus_tag="ACG8M5_000938"
     CDS             complement(1731..2414)
                     /locus_tag="ACG8M5_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0358210.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2581..3468)
                     /locus_tag="ACG8M5_000939"
     CDS             complement(2581..3468)
                     /locus_tag="ACG8M5_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358211.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3465..4334)
                     /locus_tag="ACG8M5_000940"
     CDS             complement(3465..4334)
                     /locus_tag="ACG8M5_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358212.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4471..5757)
                     /locus_tag="ACG8M5_000941"
     CDS             complement(4471..5757)
                     /locus_tag="ACG8M5_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358213.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6030..6608
                     /locus_tag="ACG8M5_000942"
     CDS             6030..6608
                     /locus_tag="ACG8M5_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0358214.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6608..7537
                     /locus_tag="ACG8M5_000943"
     CDS             6608..7537
                     /locus_tag="ACG8M5_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358215.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7534..9270
                     /locus_tag="ACG8M5_000944"
     CDS             7534..9270
                     /locus_tag="ACG8M5_000944"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0358216.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9333..10169)
                     /gene="pheA"
                     /locus_tag="ACG8M5_000945"
     CDS             complement(9333..10169)
                     /gene="pheA"
                     /locus_tag="ACG8M5_000945"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0358217.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10166..10675)
                     /locus_tag="ACG8M5_000946"
     CDS             complement(10166..10675)
                     /locus_tag="ACG8M5_000946"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0358218.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10668..11963)
                     /gene="aroA"
                     /locus_tag="ACG8M5_000947"
     CDS             complement(10668..11963)
                     /gene="aroA"
                     /locus_tag="ACG8M5_000947"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0358219.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11976..13067)
                     /locus_tag="ACG8M5_000948"
     CDS             complement(11976..13067)
                     /locus_tag="ACG8M5_000948"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0358220.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13084..14250)
                     /gene="aroC"
                     /locus_tag="ACG8M5_000949"
     CDS             complement(13084..14250)
                     /gene="aroC"
                     /locus_tag="ACG8M5_000949"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0358221.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14252..15316)
                     /gene="aroB"
                     /locus_tag="ACG8M5_000950"
     CDS             complement(14252..15316)
                     /gene="aroB"
                     /locus_tag="ACG8M5_000950"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0358222.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15349..16371)
                     /gene="aroF"
                     /locus_tag="ACG8M5_000951"
     CDS             complement(15349..16371)
                     /gene="aroF"
                     /locus_tag="ACG8M5_000951"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0358223.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17030..18004)
                     /locus_tag="ACG8M5_000952"
     CDS             complement(17030..18004)
                     /locus_tag="ACG8M5_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0358224.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17985..19868)
                     /locus_tag="ACG8M5_000953"
     CDS             complement(17985..19868)
                     /locus_tag="ACG8M5_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0358225.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19886..22111)
                     /locus_tag="ACG8M5_000954"
     CDS             complement(19886..22111)
                     /locus_tag="ACG8M5_000954"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0358226.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22226..23260
                     /locus_tag="ACG8M5_000955"
     CDS             22226..23260
                     /locus_tag="ACG8M5_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358227.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23327..24340)
                     /locus_tag="ACG8M5_000956"
     CDS             complement(23327..24340)
                     /locus_tag="ACG8M5_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0358228.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24418..24606)
                     /locus_tag="ACG8M5_000957"
     CDS             complement(24418..24606)
                     /locus_tag="ACG8M5_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0358229.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25046..25174)
                     /locus_tag="ACG8M5_000958"
     CDS             complement(25046..25174)
                     /locus_tag="ACG8M5_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0358230.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25177..26550)
                     /gene="rlmD"
                     /locus_tag="ACG8M5_000959"
     CDS             complement(25177..26550)
                     /gene="rlmD"
                     /locus_tag="ACG8M5_000959"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0358231.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26661..27716)
                     /locus_tag="ACG8M5_000960"
     CDS             complement(26661..27716)
                     /locus_tag="ACG8M5_000960"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0358232.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27726..29156)
                     /gene="gatB"
                     /locus_tag="ACG8M5_000961"
     CDS             complement(27726..29156)
                     /gene="gatB"
                     /locus_tag="ACG8M5_000961"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0358233.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29153..30625)
                     /gene="gatA"
                     /locus_tag="ACG8M5_000962"
     CDS             complement(29153..30625)
                     /gene="gatA"
                     /locus_tag="ACG8M5_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0358234.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30625..30930)
                     /gene="gatC"
                     /locus_tag="ACG8M5_000963"
     CDS             complement(30625..30930)
                     /gene="gatC"
                     /locus_tag="ACG8M5_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0358235.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31036..33072)
                     /gene="ligA"
                     /locus_tag="ACG8M5_000964"
     CDS             complement(31036..33072)
                     /gene="ligA"
                     /locus_tag="ACG8M5_000964"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0358236.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33089..35323)
                     /gene="pcrA"
                     /locus_tag="ACG8M5_000965"
     CDS             complement(33089..35323)
                     /gene="pcrA"
                     /locus_tag="ACG8M5_000965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0358237.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
BASE COUNT         9833 a         7756 c         6350 g        11561 t
ORIGIN      
        1 cttttgctga cgtctaaaaa tagaaaaaga attttcgtca tctatttgat aacgcatttg
       61 tttttgcagt tctgctgcag tcagatggat cttcccatcg tttgtatcga tcattaaacc
      121 ttctggaaac agttcaacca ctgctcgatc ccaagattca agagaaagct cgatataatc
      181 ttttactggt aattccaatt ctaccttaaa aaagtgcaaa acagccgaca gcccttctgc
      241 ttgatagatt tctcctaatg ttagcccctc tagatggaac tctggtgaaa aatgcagcaa
      301 tgttttattt tcctgcaatt gaacatatag aaatgtagat cgctcaatca gagtatcttt
      361 taaaacaact aaaaaattca tttttctccc ccttttttca tcatttttaa aatagcatat
      421 tttgaaaaga aagtctgatg cttagagtag aatttcataa aaatttacta tttcataagt
      481 attcacgaga aaaatacaaa caaaaaagcg gaaataacgc tcatccgcct tcttgtttgt
      541 gtttatttcc tcttcaaacg cttgtctatg attcagactt ttctctatcg ttattttcat
      601 ctaaaagttc ttgcattccg ataatttctc gatattgatg aatcttagtg ataccaatga
      661 ctttgtctac atcaatacta aaacaaatac cattacctgg aatatcgatg ttgatgcctt
      721 gaccaatcgc atccattact ttattcgtca aactatcggg tactaatgtc aacacaatat
      781 ctttttctgg cgaaatgtcc aaacctaaaa atttccctga atcatgtaca ccagaacctc
      841 ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt tgaaaagtcg ataacatctt
      901 cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag attcaatgct acaagatctt
      961 tcatttgctt tcccccaatt tttgcaatct ttctttttcg attctgtctt tttcatcttt
     1021 tcgtttataa atatatccta gtactaaagt ggataatatt ggtgtcaatg caatcattgc
     1081 aaccacacca aacccgtcaa tgatcggatc tcttccttca atttgtgcag cgattgccac
     1141 gctcattgat aaaataaatg tcgaacacat cgggcccgtt gctactccac cgttatcaaa
     1201 agccattgct aaaaagatct tgtccacttt tcgtcctaaa ataaatgcta gcccgtatcc
     1261 tgggatcaag aaataataaa gagagaatcc gacgagcaaa cgccacatag acaatcctac
     1321 tgctgcgccg atcccaattg aaattaccat cagcatgatt ttcgatcgga ttctcccctc
     1381 gctcaattct tcaacctgtt tgatcattac atgaatcgcc ggctcagcaa accctactaa
     1441 gaaccccatt aaaaagccaa ggggaaccag tatataattg taaggcaatc ccgcaatagc
     1501 actgcctaaa tgctgtccta ccggtacata agccatattg actccgtgca ggaaaaggat
     1561 caacccaata gtcgttatca agaaaccttt cataatgtca aagaaacgct ttttgcgcat
     1621 acgaaaacta atgatattca ttaaaacaaa taggattacg ataggtgcca ctgccaaaat
     1681 gacttccatg gtaacgattt cgattccatc tatcacttgc tgccagaaac tcattggaag
     1741 atcaccccca aaatcatcac ggcaagaatc ggccctaacg aagcaatacc gaccattcca
     1801 aaactgtcat tttcattttt cttttgtttg atcatactag tcatccctcc ggcaagggcc
     1861 aagataaatg gaacagttac tggaccagta gtcactcccc ccgaatcaaa agcaatcggc
     1921 ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc
     1981 aattgataat aggatagctt gaatacgacc cgaagcagcg caaaggctag aaatactcca
     2041 gtgccgacgc tgacgatccc aatcagcaac actctgccaa tctctccgtc agatatatca
     2101 tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata
     2161 gcaaatccca aactgaccaa aacggtcaaa cttttctttt tgatcatata tttccctact
     2221 agatctccga cttccatcat agaataatct gctccaaaca aaaagagcgt cattccgatc
     2281 atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa
     2341 acaaacgtta aaatgacaat caggacagtc atcggcaata tagcagcaat cacttcttta
     2401 aaatttttta gcatgtatcc tcctccttta ctctttaagt ttaccttaaa atgaaagagt
     2461 tttcgagcac aaacttaaaa cgttatcatt taatttttct caacataaaa taacacacgt
     2521 cccaaaagta tctttttact tggaaaaatg actttgaagg aacgcgctta ttgtgttttt
     2581 ttattttaca gagccgatca ttccttgaac gaattgcttt tgcaaaacaa aaaatacgat
     2641 ggcagttggt aaagtagcga tgactaatcc agccatgatc attccatagt ctggtgtata
     2701 ggaagcaccc atagcagata aaacaagcgg aaccgtccgt ttatcaggag attgcaaagc
     2761 tactaagggc cataaataat tgttccagct actcatgaat gtgatgattg cagccgcggc
     2821 ataggtattt ttagccgtcg gcatatacac tctaaaaaaa atacttagtt cttttagacc
     2881 gtctaatcga gcggcttcta ctagatcttt tggaaaagat ttaacatttt gtcgaaagaa
     2941 gaagatcaaa aaagcagtgc taacagatgg caggatgaca accaagaaac tgttgattcc
     3001 caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa gggatcatca atgccgcaaa
     3061 aggaaccatc atcgatagca gtagaatatt gaaaattagg tcttttcgtt tactttgata
     3121 aatctcaaat ccatatcctg ccattgacga aatcagcaat gcaaacacag tggtcaccaa
     3181 agcgatcatc gctgaattcc aaagggaacg agtaaaattc agctcattgg aaaacaaatt
     3241 ttgaaaattg atgatcaagt tactgccaat ctttattttt cccgatgtga tatccacggg
     3301 tgtattcgtc attcccaaaa ccatccagac aaaagggaaa atggaaataa atgataggat
     3361 gcttaatgct gtatacttta atactgaccc aattgtcgaa cgaagttcta atgcttgttt
     3421 tttgacgttg gggcgttcga ttgtttcttt ttctaccata tttttcatcg actttctcct
     3481 cctactttcg tttggattat cgacaatatc acaataaagg aaacaataac aaaggaaact
     3541 gctgccgcat atccaaaatt aggcgtgaat ttataactca aattatagat gtattgagat
     3601 agggtggtcg ttgcatttgc tggtcccccg ccagtaatat tttgtacttc atcaaaaagc
     3661 tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa tgattggttt taaatttggg
     3721 atcgtcactt tgataaactg ctgccattta gaagcaccgt caatagatgc tgcttcataa
     3781 atctccgaat caatattttg catgcctgaa aggaaaaaaa tcatattgta tccagtccaa
     3841 cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa ttggatgagt caaccagcca
     3901 atcggtgtgt caatcatacc aatgcccaac aacaagttgt ttacaaggcc agattgagaa
     3961 aatagacttt tcatgatcag agaataactg accatcgaag tgatacatgg aagaaagata
     4021 gcggttcgaa acaacccttt atatttcaac tttttatcat ttaaaaggtt ggaaataaaa
     4081 agtgctagaa acaacatgat cggtacttgt acaagtaaat acaggaatgt attaaaaaaa
     4141 gcttttttaa aagttgcatc tgtcaacatc cgctgataat tacctaaccc gttaaatgtc
     4201 atattgacag ggggaccact ttgaaaagac gtgatcaaag ccaaaaccat cggaataaaa
     4261 accatgattg atatcaaaat gactggcaag attaaaaaag catgcccgtt tttgaataaa
     4321 tctttctgct tcattctttt ccctccaatt ctatcattga ttatctacat gaaaagacaa
     4381 gaaagacaat atctgtaagg cagacgtgat cttttttgtc ttttcgtttg tctgatgaaa
     4441 aagcgaagaa attcactttt ttaccaccga tcaattagcc aattgtgctt ctacttgttt
     4501 ttgtgtatct gctagtacct tgtctgtctc ttctccattg atgattcgat gaagagcttc
     4561 agctacgact gcttcaattg cataggtctc atgtccgtaa ttcacttctg gtatttcaga
     4621 tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg taaaattcag aagcttcttg
     4681 ataagcttct tgttcattcg cgctaagcat cgtagatact agtccgattt ctttcgcaag
     4741 agtgcccatc agttcttcat tcgaagcaaa cgttgcttct aggaaatctt tcgctgaatc
     4801 ttctccagct actcctttga tcacatacca gccagcgccg ccaaggttag aagcttgcgc
     4861 tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc ttccattttc cagattggtt
     4921 ttctgctcct tggatcgttg aagaatacca agcgccagtt gggctagaag ctactgctcc
     4981 tgattgtatc gcagttactc cagcattcca gtcagaggtt tgttcgacta gatcttcttt
     5041 caaaagagtg gcaaacaatt caagaccata ctttaaagat gcattgtctt ggatcgtgac
     5101 tgtctcccca tcttcagatg tataccattc tcctgcctgt tgcataatca tccttactcg
     5161 accaaggtca gaaggattga cttcagcgat tttctttccc gtcttttctt tgacatctcg
     5221 cgctacttga atatactcgt cccacttcag attctccata tcctcttctg tataacctgc
     5281 ttctgccatc agatccgtac gatagaagtt tgctgttacg cccgaatcga aaggaacacc
     5341 gtatatttta tctcctactt tgtttaccgc aaatttatac ggtgcaaaat gatcttcctt
     5401 tacaatagca gacaaattag cgaatgcatc tggataagaa gtcaaatagc cttgtatcct
     5461 ataatcttca atcaagacga tattgggcaa tccttctgta ttcccgctcg ctaaagcggt
     5521 gttcagtttc tgaacgatat catcctgaga catcgtaaca acttctatct ctgtttcttc
     5581 gttctcataa actttttttg cttcattcac tgctttgata ttgaatgtct catcccacgc
     5641 ccagacagtg actttttctt ctttgtctgc tgtactacta gcacttccgc atcctgttaa
     5701 gaaacctgat gctgccgcta aacaaacgac tccagcaatt aactttctga tgttcatttt
     5761 tatccgctcc ttttccatcc attagttgtt tacgtttgct acattcatca taccaaaatt
     5821 tgaaaacggt ttctttatta ttcagatact tcaaaaattt ctcctaaaat aataaaaaaa
     5881 tacaaatcaa ctatgacaaa aggaataatc ttagcatcta gaccaaagat cgaaatggtg
     5941 aggtttcttt aaaataaaaa tatccttgca ttttctccta gaaataagta tttttaaatt
     6001 acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg cgtaattctt atcgatgatg
     6061 aacagacgat tttagatgga atgaaacagt tgatcaattg gtctgagcac gggtttcaat
     6121 taatagggac tttttccagt ccgctgaaag cgcttgattt ttctttggta catgcaatcg
     6181 atatcgtggt cacagatgta atgatgcctg aattaaatgg aatcgaatta tcgcggctat
     6241 taaaacagca acaacctgaa acacagatcc ttatcctctc tagctatgat gaatttgata
     6301 tggttaaaga ttcttttaaa gaaggcgtcg ctgattatct tttaaagcct cgattgagtc
     6361 ctgatttttt tctaaattcc ttaactgccg tttcacaaaa aatcaaaaaa aagcccaaca
     6421 aaagcccaat caccaatcgt tatgagcaga tctgcgagaa tctttcacag cagattgctg
     6481 gacaaactcg tccattgctt gaccctcttc tttttgaaca caccttgttt ttctgttata
     6541 taccgaaaaa cggccattca gttcaaaaca aggctctaaa caaaaacagt cgatcaccca
     6601 ttcattgatg gatagcgacg attcgtttac tatttatcct gtttctacct tttcggaaga
     6661 atccggctat gtaatcaata ctgattccaa agatacactt aggaagtttg ttcaaaagtt
     6721 tcgtcaagaa gtttttccag gtgatttttt tgttgtttct gattcttttg caatccaaga
     6781 tatcaatgaa atattccaag agatcagaaa ttgcaccaaa ggacaacgtt tctatcataa
     6841 gggaaagaac cttgcttatc agcatgaact tcttccgtta ttaaccaacc acgagcagca
     6901 agcaagtagt tatttaacaa aaatcgttca taaagactac gctttagctg tcaaagaact
     6961 tgagaatttc ttcacgacga tattggaaca gcctattgat ccagcttatt tgaaacatga
     7021 ggcaatcaat cgtttctatg cattactcaa cgctttggct gacgagcatg ttgggaatga
     7081 agaactttct ttgctcaaga tcgcccttcc ttctttatta gccgatgctg agtatttcga
     7141 agatttttct attcttgtca aagaaacact tgagaaactc acccactttt ctgcaaatgc
     7201 aaaaaaagac aatttcgata tcttgacttt gatctacgag ttcgtccagg aaaattatga
     7261 gaaagaaatc aatttacaac ttttatccga aaaatatcat ttttcatata gctatctctc
     7321 agccatcttt actgaaaaat ttggtatcaa cttttcaaaa tatctaaaaa aagtccgtat
     7381 cagtaaagcc aagctctttt tgacggaaac acggcttact ttgacggaaa tctgtgagaa
     7441 agtcggctat accgaactgg gttatttctc cagagttttt aaagaagaga caggaatgac
     7501 accttctcaa taccgtaaag gaacttttgt aaaatgagaa aaaaaatcaa agagcatgaa
     7561 ctctttgcca aacttctgtt cattttgttc atcggtcttt ttctgcaagc catcgtcatt
     7621 tctttgttta tttatcatcg atcaagggat gcctatatcc agctgttcaa tcaatcgaat
     7681 gatgtggtac taaaaaagat tcaatctgaa tttgaaagtt tgaatgatac aatcgagaat
     7741 acattagccg cttttgattc taatccagct gtcaaaagct atttttccaa tgatccagcc
     7801 caacatatgg aacagcttca gcaactaaga acaatccaaa aaatgaatga ctctctttca
     7861 aaaatccatc caatgatcga ttacgatgta ttgatctttg gagaaaatgg aagaactttt
     7921 gtcggaaacg acatgctcac tgccgtatct gccgattctt tttttcaatc tgcgattgct
     7981 cagagagtga atgaacgtgc tgctgataca caaatgcttt ttgcacacca cggattgact
     8041 cttcgcgata agaaagcacc cagcgtcttc tttgtcaaaa aactgaaaaa tacattgaat
     8101 cacgtttacg gatatgctgt tttatcgatc tcatccaagc aactagcgaa tctttttcaa
     8161 tctgtagtaa atcctgagat atcgaagatc tccttcatga acgaagaaca gacgatcatc
     8221 gcatcaaatg aagaaaatac aattgggagc cgatcaatga ttttcaaaaa actaacagta
     8281 ggtgaaacaa cgatttcaaa tggacagcag ttgacccgtc ttcctctgta ccgccaggat
     8341 tgtacattac tcagtcaagt cgatcttcat tctttatcta accaaatggg cgtgattttt
     8401 ccgattattt tgtataatat cgtgacactt gcttttgctg gctcattggt atttatgtat
     8461 ttggatcgac acacaaaatc gatctaccgc ttgattgatt cgctaaaacg gctagaggaa
     8521 gatggcttaa aagctactgt tcctgtacaa ggaagccatg aagtccaatt gctagggaat
     8581 acgatcaatc aattgctaag taatgctcag catcattatg aacagacgat ccaagacgaa
     8641 aagaagaaac gttcgcttga aatccaaact atgcaagcac agatacagcc ccattttatt
     8701 tataatacgc tgactagctt gaaatttttg atttggcaga aaaaaacaga tgaagcagtt
     8761 caaggaatcg acagtttcat tactttacta agaagtacga ttgggaaaaa agaagaagtg
     8821 attccagtca aggaagaaat caaaagcgta caaagttaca tcgatctttt gagtatgcga
     8881 tatggcgaaa gaatctcggc taaaataatg attcctgatg aactgcagtc attatctatg
     8941 ccgaatatga tccttcaacc aattatcgaa aacgcctatc ttcatgcttt tcacaataaa
     9001 acgcaaggat tcatcacggt gtacggcaaa acaaaaaatc agcttctttt attcgaagtg
     9061 attgataacg gcgatggctt cgatactgaa aataccaaac aaaaaaatga tcatttttca
     9121 ggaatcggca tcagccatgt agatgagcgg atacgcctaa tgtatggcga tgcatatggc
     9181 ttaacgatcc aatcagtgat tgggacagga acgactgtaa cgatttgtct accgcttctt
     9241 cctaaaaatc aatcagaata tcaaaaataa agaatagcca agcgctgatt tttttatttt
     9301 aagttaaata aatcaatcag tattcttgac tttcatctac gcgtctcttc tactgtcagc
     9361 actggataac agcccaatat ttgactttct cctccgagca tcttgatttc ttggagcgca
     9421 ttctctacta atgtcattgg acggtccaac aacaaatcga tcacgaaaaa atattctccc
     9481 aagctagttt tcaatggtcg ggattcaatc ttacttaagt tgatttcccg ccagccaaaa
     9541 gctgccagca ttttatgaag catccctggc cgattagcag gtagtgtcaa gatgacactc
     9601 atttttactg gagctgactg ctggctggtc atctttcggt caccgacgat ccaaaaacgt
     9661 gtctggttca actcattatc ttggatattc tcagctaaga tctccaatcc aacatgctgt
     9721 gctgtttcta atgaagcaat cgctgctgcg tcttctttcg gatgttctgc tacatacatt
     9781 gctgcagcag ttgttgattc tgttgctact aaaggaacat taggataatg cgtttctaaa
     9841 aactgttgtg attgtgctag tgcttgtgga tgtgaaagga tttttgtaat ctttgttgct
     9901 ggattgccta aaagctgctg cttgatagga agaacgattt cacttttaac ttcgacttct
     9961 ggatgtttgg aaagaagatc gattgtgtga tgaacagctc cttccaatga attctctaca
    10021 ggaacgacag ccagatcgac ttgtttgcga aacaaagcgc gcaaacaaac agggatggaa
    10081 gcatacgcag tcaattgttc tgtaggaaat agctgacaag ccgcctgata agtgaatgaa
    10141 ctttctggcc ctagatagct gacgttcata tgcttttgat cctttctata atggtatcga
    10201 tgatcttttc tggtgtttga ttggtcgtgt tgattcgatg tgtcgcactt tcttcatacc
    10261 aagaaagtcg ttgtgccaat aatgctttta tttcttctct tgtttttcca agagctaaag
    10321 gtcgaataga cgtttcatct tgttggatac gatcaactag acagtccgct tctgcctcta
    10381 agtaaagaac aatcggctgt gtttttaaaa aacgccgatt ttctgcactt tggacaatcc
    10441 ctccaccagt cgcgataatt ttgtcttcat ttgacagctt tcttaaaata tctgactcca
    10501 cttttcgaaa gcttttttca ccgtaatagt gaaaatattc ggcaatcgac aattggatat
    10561 gttcctcaat ctttgtatct aaatctacat actctttttg taaacattga gctaaccctt
    10621 taccgattgt tgtttttcca gcgcccatga aaccaatcag caaaatgcta gccatgagct
    10681 tctcctcctg acacaagttc tgctaaatcg tcaaaaaatg ccggataaga gattgaaaca
    10741 gcttcagctc cttctagttc gcaaggtcca tcagctatca aagcagctac ttgcagcatc
    10801 ataccgattc gatgatcgcc tctgctggac acttttgcgc catgcagagg tgtcggccca
    10861 tggatgatca gaccgtctgc tgtcgctttt atctttgctc ccattttctg taattcttct
    10921 gccaccgcat caattctgtt ggtttctttt acttttaatt cttctgcgtc tttgatgatc
    10981 gtgattcctt ctgcttgagt agccactaaa gctagaatgg gcaattcatc aatcagacgc
    11041 ggaatgattt ctccaccaat cgttgctttc ttcaaatgac tcgtcttgac acttaaatca
    11101 gccgattggt tctgttcatt gtgattcgtc tgggtaatcg ctgcgcccat ctcctccaac
    11161 acatctaaga taccagttct tgttggattg accccgacat tttttaacaa cagttggctt
    11221 tccggaacaa gaagtcctgc tgctagaaag aaagcagctg atgaaatatc acctggaaca
    11281 gttatctgtt gaccagttaa tttttgcggt ccggtcacta tgatcgtttt atcttccact
    11341 gtgatccttc cgccaaattg acgaatcatt tcttccgtat ggttacggga acgttctttt
    11401 tcaacgatta cggatgtgcc ttctgcttgt aatgccgcaa acagcaaagc agatttgact
    11461 tgcgcactgg ctacaggcat attgtatcgg ataggtgata aagagcgggt tcctgtcaca
    11521 gtgattggta gatactggtc attttcttct ccttgcaagt cagcgcccat ctcgcgtaaa
    11581 ggttccatca ctcgccccat tggacgttta gacaacgaag catccccgaa cagagtagtc
    11641 gtaaaaggct gtccagccaa aatacccatc aataaacgta tggtcgtacc ggagtttccc
    11701 atgtcaattg attcggttgg ttggacaaat gagtggctac cgcgaccatg aatcttgatc
    11761 acctcttcct cttcttcgat ctcgacacct aattgttgga aggcatgtaa agtactcaga
    11821 caatcttctg cgcgcaaaaa attttgtata gtcgtcgttc cttcagcaat tgctccaaac
    11881 atgatgctgc gatgggaaat tgatttatct gctggtatcc taacagtccc tcttaatcca
    11941 tgtatttgtt gtaataattg catttatcca gcctcctatt tgatccagca atgataactg
    12001 gtattagaca taatgattct ttttgcttgt aacaaatcct gttggttttt aaaactgatt
    12061 tgcagtattc ctaaaatatc ttccctggtc tcttggatct tcaaattaat caaagaaact
    12121 cccgcttttc cgatcagtcc agttacttct gcaataacgc caggttcatc tggcacatcg
    12181 ataaataaat caaaaaaggc aggaatcgcg ccttgcttat ggacaggcaa ctggtctcga
    12241 ctttcttttg cccgattgaa aaaagaaaag atttccgatt ggttctcagt catgatccat
    12301 tctgctattt gtgtcatttc ttgttgccaa tctgccaatc gttccaataa tatctgctta
    12361 ttgctcaaca aaatatctgt ccacatgaca ggatctgagg aagcgatcct agtgatatct
    12421 cgaaagcctc ctgctgccag ttgtttggct cgcggatact cttgattgaa ttgttctgcc
    12481 tgattgacta agccagctgc cagaatatgt ggaaaatgac ttagcattcc tgttatacgg
    12541 tcatgttctc ttgctgtaag agttacatat tttgctttgg ttccgcaaaa tagttcttgc
    12601 agtttttcaa catctttttt cgtcttttcc aaaggtgtaa agatgtaata ggcattttca
    12661 aatagtttag catctgctgc ctgtatacct gatttatgag aaccagccat tggatgtcca
    12721 ccgacaaacc gaagattttt ctgctcagcg gatgtcacga cttgctgctt cgtactgctg
    12781 acatccgtaa tgatgacgtg atcagacaag ttgattcttt caaggacatc aagataagct
    12841 aaggttgtac ggacgggaac ggctaataag ataacgtctg cttgttctgc acctgtttca
    12901 aaatcacttg caacgtaatc aacgatctgc tgttctactg cttgctcacg ggtttttgac
    12961 tgactatccc aaccaactac tatcagttct ggattggtct gacgaataca aagtgccata
    13021 gaagcaccaa tcagtccaag ccccacgata aaaacatgtt gtgccatctt atttgccccc
    13081 tttttagtaa tgtttcaagt agtcacggta ctccttcact gcagctttca actcaacaag
    13141 cgaatcactt gggaattttg acaatagctc tgtcgctacc acagtggcta caacattttc
    13201 cgcaacgacg ctagctgcag gaactgctgt actatccgaa cgttctacac ttgctttgta
    13261 aggttctttt gtatcgatat caacactttg aagtggcttg tacaatgtag gaatgggttt
    13321 catgaccccg cgaatgacaa gtggttcgcc attggtcatt ccgccttcga agccgcctag
    13381 attattggta cgtcggaaat agcctctttc ttcttcccat aggatctcat ccatcacttc
    13441 tgagcctggt aatgaaccag aagtgaatcc gaccccaaat tctacgcctt taaaggcatt
    13501 gatactaacc acagcttgtg cgattttggc atctagcttc gtgtcccatt gtacgtagct
    13561 tcctaatcca gctggaacgc ctccaacgag cacttctaca attcctccga tcgtatctcc
    13621 atttttcttt gtctgatcga tcaaatcttt tattctttgc tcaactgctg gatcaacaac
    13681 tcgtacttct gacgcttcag aaatttcttt gatttcttga atcgagatat ttccaggtat
    13741 ttctgcttgg attccaccaa gacaaatcac gtgtcctgct acgatgatgt caagttcttc
    13801 cagaattttc ttggcaattg cgccgattgc tacacgcatg gtcgtctctc ttgcagagga
    13861 ccgttcaagc acattccgaa gatctcgatg atggtatttc ataccgccta ccaaatccgc
    13921 gtgtcctgga cgcggacgtg caactcgccg cagtgattgg tctttttcag gcacttctgc
    13981 tacagacatg acagttgtcc aatttttcca atctttattt tctaccacta aagtgacagg
    14041 tgaaccaatt gttttgccgt gacggatgcc agacgtgatc cgaacacgat ctttttcgat
    14101 cttcatcctt cctcctctgc cgtaaccagc ttgtctgcga gcgagttcgt gattgatttc
    14161 ttcttctgac agaggtaatc ctgctggtag tccttcaata atggctgtta attctggtcc
    14221 atgggattct cccgcagtca tataacgcat cttacagtcc tcctaagtaa tctttgaatg
    14281 tctctgtggg aacaggaacg atttttgctt gcccaatctt ttctagcaag atgatattca
    14341 attggcttcc tcttgctttc ttatcatggg ttatcgcgtg gaataattca tctggatgac
    14401 ttagatcgta cgtaatcggc agatggaatt tttgaagtat cctttctaaa gcagctgtcg
    14461 atccagttgg agacaagccg attttttctg catgagaagt gatggctacc attccaatag
    14521 ctactccttc tccatgactg actaccccat atcctgctgt attttcaatt gcatggccaa
    14581 ttgtatgacc aaaatttaaa ataagtcgat ttccttggtc aaagacatct tcttccacta
    14641 cttttcgttt gatttccaac gtagcagtaa tgattggaac agcatgttcc agaagatctt
    14701 tttcgtcttt cagctcgttc agctgtttcc aaagcgttgc atctgctata gctgctgatt
    14761 tgatgatttc agcgattcct tctcgtaaac gacgtaccgg caaagttttt agaacctcag
    14821 gatcaatcaa tacaccagct ggctggctaa atgttccgac aaggtttttc gctttgtttg
    14881 tattgactgc tgtcttcccg ccaatactag aatctacttg tgcaagtagc gtagtcggaa
    14941 tttgaacgaa atggatccca cgcatgaacg tcgaggccac gaatgctgct agatcaccca
    15001 ccattcctcc accaagtgca agtatcccat cgctgcgggt aaaatcatta tctgctaaaa
    15061 aatcgtataa ctcagctgct tgttctaaag atttactggt ctcacccgcc ggtacgattg
    15121 aaaggactgg ttcaaaatta gcttgtctca attggaacat gattttttct gcatatagtg
    15181 gagcaacgtt tgtatcagtg atcacagcta ctttttgagg tctccaaatc gtttgaagcc
    15241 atgatccagc ctgttctagt gcgccttttt caatatgaat atcgtatttt tggttatcaa
    15301 catgtacagt aagcattcgt ttcacttctt tcttttaaaa agctggaatt aatcgagtgt
    15361 ctgaatcttt tccattgctt ctttcatgat cgcaacttct ttcatcatgc gtgagtaagt
    15421 cttttcattc aacgattggg gaccgtctga ccaagcattt gcaggatcag gatggatctc
    15481 tacgatcatg ccatctgctc cgcttgccac acccgcacga gccattggtg gtacaagttc
    15541 ccaaattcct gtaccatgac ttggatcaac aatcacaggg aaatgagtta attttttcat
    15601 caatgggaca gcacttaaat caaacgtatt acgtgtcgct gtttcgtaag tccgaatccc
    15661 acgttcgata aaaatgacag gagatttgtc ttcgactgca atgtattctg cagcattaag
    15721 ccattcattg atcgtaccgg aaattccacg tttcaatcca acaggtttac ctgttttacc
    15781 gacagcagat agcagtttaa aattttgcat attacgtgcg ccaatctgta ggatatcgct
    15841 gtactcagcc accatatcga tatgcccttc atccatcact tcagtaatca ctttcattcc
    15901 aaattcatca gccgcttgac ggatgtattt caatccttct tcttctaaac cttggaaagc
    15961 atatggagaa gtacgtggtt tgaaggcacc acctcgcaga atttttgcgc ccccggcttt
    16021 ggccattcgc gcacattctc tgatttggtc taatccttcg atagaacaag gaccagccat
    16081 cgtcacgaaa ctgccgtcac caatttttac tccgtctaca tccacgactg tattgccagg
    16141 atggaattct cgagaagtca atttgtatgt gtttaaaata cgcacagcat tttctactcc
    16201 ttcatagcgt gtaaatgctc cttcttgtaa acttcgcgta tcacctttta gtccaatcac
    16261 tactcgttct gttccttgat cgattgagac tttgaatcct tctttttcga tatgatcgac
    16321 gacttttttt atttgttctt ccgttgcttc ttttttcata attactatca ttgtttttcc
    16381 cgctttcttt tgtttactta tctatcttgg ctgattttct ctactttttc aaggtggttt
    16441 tacattaatt cctactaaat gaccgtgtcc tgttgttctc attttcgctc cttctttcct
    16501 acttatgacc cagataatca acaaaaaaag atgtccctgt aggaaattgc ctctacagtg
    16561 aacatcttca acgagcctta aaaagcgctg caaaaatata aaatagccac tatagatgaa
    16621 tttctctacg tggctgtaag tcgtgtttaa aaatctttag ccatcataga tacaaacctg
    16681 gtttgtttgt acccacgaag tcatgaatac aaacctatct aaaatagcta aagtaatacg
    16741 tattcagttt tgtaagtttt ctagatttca taaaaaatcg ctccaaactg atgtaagaat
    16801 tgtacctagt atagacataa aaaaatcgac tgtcaatcat ttttatgaaa ctttttttaa
    16861 ttttctgata gttaactttg tttttcagaa aaataacaat tggttttatt gaagaaaata
    16921 gtcatgaaca aaagtatctg agaacttttt tactctcttg ttcatgacat atagaataaa
    16981 gcagctttgt cttgtaaact ccgtttttca aataataatc taaagattgc tactgaataa
    17041 taaagccgat ttcatggtcc tcttcgctgc tgattcggta aggtgactca acatccgctc
    17101 cccagctatt gatacccccg actcctctta cttttgcaaa aatcgtaaga accgttcgtc
    17161 tttctggcgg tagttcttct atatgtgttg cctgttccag ttcttctgct ttgaatggta
    17221 aagcggaaaa agcaaatggc gtatcctgct gttcaaatcg caacgaaaaa tcttcttgat
    17281 ctttatccgt attatccaac gtttgatttc ttgttagttc tacccatttc gtttccatat
    17341 gcatgccgca ttcttgggga acaaggtatg gggaaacggg gagtcctgcc actttataaa
    17401 taccttcgac accaccagcc atccgatccg gatatgtttc ccctgatagg ccttcatact
    17461 caaaaaattt tgctttcgtt ggcataatga accttaaacc caatgcgggt aattccggta
    17521 atcctttttg tccataataa tgcatattga caagcatttt accctctcca gcgatttggt
    17581 aggaaattgt cacttgggtt tttggtactg tgttgatttc cagtgtaaaa atgatttcaa
    17641 ccgtttcagc gtattcatta cttgaatatt gattatttac tggagcaatc ggcaaatcga
    17701 tctttttatt gtccaccagt acttctattt ctttgacttt tggatacaat ccggctccta
    17761 accacatacc ggatttaaat ccaaatccgc tacctctgtc attatcggtc gttgctcgcc
    17821 aaaaaactgg aagtgtttcc cggtatagcc attcttttcc gtttttattg agcgattcca
    17881 gtccgcctct tgtataagaa aaaatataat gcgtattccc cgatttgaca cctaatgtac
    17941 agtcgccgaa gacgattgca actttttccg tatccatatg acttttattt actgtatttt
    18001 tcataataat atcttacctc ctgcatggcg ggtttttctt gtccatctgc aaaaacgatc
    18061 ccattccctg agaattcata gtcacttggt ttatcgtcaa agtcaccgcc atagcgcatc
    18121 acttctctgc ctgtgattgg atcagtcact tggatcgcct gatctttaaa atcccatata
    18181 tagcctcctt ggtacatttc ataacgatca agcaacgtca tgtaagaatc cattccacct
    18241 aacgaattcc ccatgtcatg catatattca cataagatga acggtttttc cgctttattt
    18301 tcaaggtagt caatgatctc ttctggtggc gcatacatgc agctttttac atctgaaatc
    18361 gaatcttcga atgctggatt tggaaaattc ccttcataat ggacaagtcg atcggggtct
    18421 acttttttga agtaatcgtt catttgtcga atgttttctt ccgcataaga ttcatttcca
    18481 agagaccaaa aaagaatact tggatggttt tttaatgttt cgaaattcgt ttttgctcga
    18541 tcaagaacag ccgcctgcca ctgaggtact gcacctggta cgttgtaaga aggctcaaat
    18601 gcgcccattt tttgccatga gccatgggat tcgaggttcg tttccgccat cacatggatt
    18661 cctgcttggt cgcataaata ataccaagtg atctggttgg ggtaatgaga tgtccgtacg
    18721 ccgttgatct ttgcattttt tatgatagcc aaatcttttt ccatatcttc tatagtgatg
    18781 gcgcgtcctt ttttcggatg ccactcatgt cgatttaccc cgttgataat catccgtttt
    18841 ccattgaagt agatgatttt atctttgatt tcgattttac gaaaaccgaa ggaatagtca
    18901 atatactccg ttactctttt ttcttcgtct atcgtctcca attcaagctt atataaattg
    18961 ggctgaaaat gcccccacag tgccacattt tcaaatccat cgatgcgaat cgtcatttgc
    19021 tcttttaacg gatattgctg ttgatagatt tccttcccat caggatctag tatcgctaca
    19081 ttcagctgac tgtttttggc tacttgctca aaagacaaat ctacttcaag tttgccattt
    19141 tgacatttgt catctaagcg aggtttgatc caaagatctt ctaaatggtt ttgtcctcga
    19201 gcaactaaga agacatctct gaagatccca aaaaatcgga aaaagtcctg atcttccaag
    19261 aatgcagctg tacttctttt gtaaacctcc acagctagaa tattttcttc ttccaaataa
    19321 ggtgttaaat caaattcaga cggagtgaac gaatcttcgg catacccgat aaaatgacca
    19381 ttcaaccaaa taaacattgc ttgttctaca ccagcaaaat gaatcgagat ttgttttccc
    19441 tttagacctt cttctaatcg aaaagtcgtc aaatacgaac caaccggatt gtctttagca
    19501 gctgaaaaga tcccttccgc ctctttctca gggcttgtcg tgaaaggggg acggcgaaat
    19561 acatgtccct cccacggata catcgtattg atgtaatgga tcttgtcata atttgctaat
    19621 tcaatatgac taggaacagt gatttcatca aaagaacagg catcaaaatc ttttttataa
    19681 aatgtttttg gtcgtaccga aggatttggc gaataacaaa aacgccatga tccatttaaa
    19741 gactgtttaa gggaggatgt ggtttctact tcttcccttg ttgcataaca aacatgatcg
    19801 ctatgtgcat ttgattggtt cactctaaaa acttgtggat catctaacca ttttatattt
    19861 gctttcatac ttttttcctt ctctctcata actttttgaa atgaagcagt tctgtttgga
    19921 aatcaccttg aaaatcgggg aagtaaagtc caactgtcat caattcggcc cccgaaaata
    19981 tccgcttcga ggtctgttcc tgataaagtg cctcatcatc taaccccatc agatatactt
    20041 catggaacgc tggttgagct gaagctagta tcctccctaa aaacacaata gcttcatctt
    20101 gtttaggaga cacaaacatc catgcggcaa tattttcttc aaaaggactc gccaaacgat
    20161 aataatggcc atactggatc accggacgaa tcgtttgata ggatataatt tggtctttta
    20221 cttgcttttc ttcttcagga gacagctttg tcaaatccag ttcataacca aaaacagcgc
    20281 tcatagctgt ttctcctctt gttttcaacg aagtagatct gtcggtctga tgatttggta
    20341 cagctgatac atgagctgtg atggacgaaa tcggataagc aaggcttgtt ccatattgga
    20401 ttttcatacg ctccactgca tccgtgttat cacttgtcca tgactgcggc atataataaa
    20461 taaacccggc atcaaaccgt ccacctccac cagaacagcc ttcccataag atttccggat
    20521 aatcagttgt caaatgttca agtagttcat aaagcccgag catataacga tgcgctactt
    20581 ctccttgtct ctgtgggtca agggtaatcg aatagacatc tgaaagcgaa cggttcatat
    20641 cccatttgat gtagtcaaga ggaatcgtat ctaaaatctt tctcatttgt ttctcaatcg
    20701 tctgccgaac atctaacctt gtaaaatcca gtacatgttg tgatctggaa gcagaaggca
    20761 ttctacctgg ctcctgcata agaaaatcag gatgtgttcg atacagttca gaatcaaccg
    20821 agatcatttc aggttcaaac cataaaccaa atttcaatcc tttgctatga gcataatctg
    20881 ctatttcacg taatccattt gtcaacttgc cttcatactc gaaccagtct cctaatgaag
    20941 aattgtccgc atctcttttt ccaaaccagc catcatctaa tacgaacatt tcgatgccta
    21001 gctcctttgc ttcatcaatg atcgccttga ttttttcact attgaagtca aagtaagtgg
    21061 cttcccagtt attgaccaga atcggccgtt cagcaaattg atgacgacct cttgcgattc
    21121 tttcacgtag caactcatgg tggatctgac tcattttgtt caatccctga cttgagtaag
    21181 aaaggatcat ttcaggtgtt tgaaaagact ggcctgcatt caattcccac gaaaaacgat
    21241 ggctgttgat cccaccgacg actctcaatt gattgatctg atctttttca agttcgaaag
    21301 aatggttgcc ggaataaaca aattgaagtc cgatcgcttc tccagtattt tctgtcgtat
    21361 gatgatgaac taaagcaata aagttattca tatggtgact gctcgttcct cgacggcttt
    21421 caaatacttt tgttccagat aaaacagatt gccgtgaaat ctgtctttca ttgacatgtg
    21481 ctcctggcaa agcaatgact tcatcaaatc gcctagtatg ggcaaagtct agctggaaac
    21541 tagccgcttt ttctagtttg attgtttctc cggtttcatt tcttatcttt acagatcgtg
    21601 tgatcactgc tctatcagta aatatggtat agaacaaatc gaaataaatc gctgtttctc
    21661 tatccttaag tgaaatcacc aacgtttgtg cctctgactt gtccatcaca taagtttgag
    21721 gcaagccttc gatctctggc ttcccatctg taatataatg gctgtcatat ctcagatcca
    21781 gtaagtctga tccattcttg cgctgaatga tcgatgcggg cgttcgaaag tccatgctgc
    21841 catgagaaga gtattcttgc ggtagtgtat ctttcgacag agaccggtca ggatttaagg
    21901 gaacatttcc tgaaaatcca cgatctcttc ggggataatt tttattgtct ttgtactgct
    21961 tgactggttt gccaaaataa atatgcgcca atacattgct ttcttctatc tttatcagat
    22021 aagaaagaaa tgtatttcgt aaatggaaga ctcggctctt ttcatcaaat aagatgaaat
    22081 ttttatctgt ttttctatgc gtaatatcca tcatttcact cccaacaaaa cggaaaccgt
    22141 ttactttttt ttataattcc tgtatactgt ttactaaaca attttgtcaa taaaaatttt
    22201 ttattttttt gaggagtgtg taaaaatggt tggaattaga gatgtcgcac gaaaggcaaa
    22261 tgtctctcct gctacagttt cccgtgtgtt gaataacgat cagacgatcc atgtggctca
    22321 gactactcgg caacggatag tagaggcagc tgctgcgttg gattatgaca tcagtactcg
    22381 taaatatatc aaaaagagaa tgccttcgat tggtgtgatc tctacgatcg gcaaagcgag
    22441 cgaagaacag gatatctatt acaaacattt gcgagtaggg atggaagaag aagctcgacg
    22501 tctgcattta gggatgaatc gaatctataa tctatctgat aatcctaaac aatgggaaga
    22561 tctagatcag ttaggtgcgg tgatcgttgt tgggactgtt acgaaagaat cgattaccgc
    22621 cttgttaaaa caaaacatgc aaatagtcgt cgtggataac cctgacatcc agcaagaggt
    22681 cgatatggtg tatgcagatc ttgaacggat gactaaaacc gtactggaat tgttcttaga
    22741 aaatggtcat aaaagaatag cctatatcgg gggatataaa gtcgatatgg atgaattcgg
    22801 tgggaagcag atttctataa atgaaaagag gatgcgtgcc tatcagcatt ttatgatcga
    22861 tcatcactta gaaaaatata tgtcttatca tctaggtggc tggacagaaa aacaaggtga
    22921 ggtacttgcg actaatctat tagaaaatga atcccctctt cctactgcca ttcttgtcgg
    22981 gagcgatcca atggcaattg gtgtctatcg ggcattgaaa caaaaaaata tccaaattgg
    23041 agaagagatt gttgtagcaa gctttgataa tatcgagaca atcgcaaacc tctcccctgg
    23101 cttgaccagt gttcaaatca atgcgaaatc aattgggcaa gcagctgttc gaatggcatt
    23161 ggagcgaatt gacggtatac gggacgaacc aatcattttg acttatcctg cacgattgat
    23221 tgttagggaa agttttatcc caagaacaaa aaggtattga aagagaggtt gtgaaaagcc
    23281 gcttcgtttc ttcatactca aaacgaaacg gctttttcac aacctcttac ttatacttat
    23341 tcacaaaatc caatgtctgc tggtaacaat tttgcgattc ttttagcgtg taatcaaatt
    23401 ggtattcatg ggtaatctct ttttttgtat ctgtataaaa caaggattgc acgggtacct
    23461 ttaattctgt caaccgattt tctaatgctt gaccttgctc ctggaatgag tatgcatttc
    23521 cgtcagttat atacgttggc ggaaattcct tagaaacatg gtcagccaca ctagcttctt
    23581 tcaattgctc actcgttttc caatcttttg ttccaatcaa cgaccacgca acggttttga
    23641 cgaaaaactt cataaatcga tcatccgaat gctgctgtgc tgttagcttc aaatcaacag
    23701 gtccgcaata agaaatggtt cctttcagac tatcttttgg aagtagctgt ttgattttca
    23761 tttgtttagc atacgcatca ttcgtttgta tagctgcata ttgcaatgcg atttgtgaac
    23821 ctgcactatc tccaccaaaa aataattgtt ccatgtctag caatttgtcg tctttttctt
    23881 cagctaataa atctttgatc agttcattca cttgaagaac ttgattaggg tattctgaat
    23941 caggcgctaa ttcataattc atcgcaacca ctgcgatgtt ggcgtctgaa actaattttg
    24001 tcgcaaattc tttgacaccc gatttatctc cgccgacgaa tcctccacca tgtacccaaa
    24061 atagaacagg aacaggcccc ttgctgtttt ttggatagta gatgtcataa gtattctctt
    24121 tatacttgga ctcatacgtc aaatctgttt ttttcgccac gtttttttct gcttgatcga
    24181 acgttttttt atcagtgatt tgaacagtat ttgaaaacat atggttgatc aagaatgctc
    24241 caggtctggg ggaaagctgg aaggcaagaa agattgcaaa cgcgatccca agaatgattc
    24301 cgaagaacca tccgataaat ttaagaattt tctttttcat aaggctccct cagtagttag
    24361 tattaagcat tctgattata ccatagtgtt atttcctatc aagctctcct agatgattta
    24421 atgattttcc ccaaacttct tctgcaatgc ttttgacaaa agaaattttt tccattgttc
    24481 ttcaaacaaa acattttctt cttctgttga agcaaatcca cattgaggac ttaagcacaa
    24541 acgatccaaa ggcagatatc gtgtcgcttc gtagatccgg tcaatcactt gctggtgctc
    24601 ttccatttcg ccatttttcg ttacagtcaa aatctaagac aactaattta tcttcagaga
    24661 ctttctctaa aagaacaaaa ctcccagcac gttcactatc gtattctaaa taataggcac
    24721 ggacattttc ttgagtaaaa taatgggtct gctacgtcgt catcccccct gatgccgcca
    24781 aaatcgaata ataatttccg cgtcaaacat gtatatttag tgttgaatca tttgagaata
    24841 tgaaaataaa aatctttttg atcaatcagt taatcaaatt gaaataacga aaaatctgag
    24901 acagaatgac gttcattctg acccagattt gattttgcac aactcaacag tccttattgg
    24961 caaatcgcca aaataatgta gactaactaa aaagactaag aaacaataac aaaagttaac
    25021 acttcttagt ctttctttag cacgtctatt tattaaagat tttagggcct tttctcatgt
    25081 ttttacgatc attcttaggt gtttccattt gtttggatcg gcctccacct tgtttctttt
    25141 tgatcaattc tttcattttt tctttttggt ccatttttat tctcctttaa cgaatgaagc
    25201 gactaattcg atatgatggg tttgcgggaa aagatctaca ggctgaactt tcttcacggc
    25261 ataacctgct tctgcaaata acttagcatc tcttgcaaaa gttgccggat tacatgagat
    25321 atagacgatt cgttctggtg acatttcgat tgctgattca ataaaatgat gatctaatcc
    25381 ttttcgtgga gggtcaacta cgatcacaga tggtttgatc cctttttctt gccatttttt
    25441 cataactttt tctgcttttc cgacttcata atgaacattt tcgattttat tatttaaagc
    25501 attgaactgt gcatcttcaa ttgcttctgg aataacttcc atcccataca ccttatccac
    25561 tctgtctgct aaagacaagc caatcgtacc tattcctgaa taagcgtcga tcacaatgtc
    25621 ttttttatct aactcggcaa actcgatggc tgtttggtac agatactctg cttgttgagt
    25681 atttacttga taaaatgact ttgctgagat tcgataggtt tttcctaata gctgatcttc
    25741 gatatagtct ctgccatata aaactttttc tttgtctcct aaaatcacat ttgttttttc
    25801 ttcattgata ttttggatga ccgaaaccac ttctggcaat tcttcatgaa tagcttcagc
    25861 aatttccttg gatttaaaga atttttcttt acgagtaact aaaacgacca tcatttcatg
    25921 ggaataatga ccacgacgga taatgatgtg acgaagctgt ccttcatttt tttcttcatt
    25981 atatcctcgt acttggaacc gttgcaagat atctctgact actttgattg cttgatcgat
    26041 gacaggatct tggatataaa aatcttcgat agggactaga tcatggctat tctttttgta
    26101 aaaaccggtg gctaactgtc catcgatacg gcgaacaggg atctgtgctt tattacggta
    26161 gccgaatggt tctgccattc cgaccgttgg aagtacagga acttctggca tcttagcaat
    26221 cttcgccatt acatgctcta cttgtttttg tttgaagaca agctgttgtt catacttcaa
    26281 atgggctaaa ggtgcgatac ctatgcgaag taatgcattg ttttctacag gctgacgatc
    26341 aggtgattgt gtaataaatg tttcgacttt tccatatcca aacttattgc ctgcttttac
    26401 tactagtatt tctacttctt ctccagggag tgcattttcg ataaataatg gataaccatc
    26461 gatttttgct acccccattc cttcataact caagtcgacg atttcgactg tatatcgttc
    26521 gtttttcttt actatttgct gttctgtcat attttgctcc tttaagccta tttttcaatc
    26581 ctactgaact ctactataaa tcagctaatt acacaactaa aaccggcact gaccttacta
    26641 cgttcagcac cggcttttga ttaattcttt tgattaggtg tttcgtctgc tatttttccg
    26701 tcaccgtcga tatcttcatc cgttaaacgt tcgacttctt ttacgaattc tttgctgaca
    26761 acctctaatt cttcttcatc tgtacctgtg attgcttcat caggaatctg atcggtattg
    26821 gcaaagaact caatgtgttg atgaagattt tggaaatgca tcggtgcttc cccgccgtat
    26881 tcaccatcta agttgatcat tactggtcga ttgatatcat cttctgtttc aacgaacaaa
    26941 ctgcttgttt tggtatagat tattcttgga tcatcgacat gtcgaccgcc attcagcatg
    27001 agtgctgcaa tatgcaagat ctcaaagata tttgctgtct tcacaatgat taaagaaaac
    27061 ttcccatcat ccaatttggc atctggcgcg atctgttcga atcctccaac agagttcgtc
    27121 agccctagga aaaacatcga agcatttccg atatattccc cttcatcata cttcaagcgc
    27181 attttgattg gctttactcg agggagcatc tctgctcctt tcgcaagata agcaagataa
    27241 ccaaaaatac tctttagttc tgatggaact tcataagtca attctgtcaa atagccacct
    27301 gcagcgatat tgatgaagta actgttatca gaaaggccga tgtccatttt tacggtttgg
    27361 tgttttttta tcacttctgc tgctgcttta atattatctc taggaatttt taatgctcga
    27421 gcatagtcgt tcgttgtacc agctgggatg atggccattt taggacggtg ttcaagcgga
    27481 gcgattccat taaccacttc gttgattgtg ccatcgccac cagctgccac tactagatca
    27541 aaacctagtt ctgcgactct acatgcttca ttcttggctg aatctggttc tggtgtagtt
    27601 gcataggcgc tggcttcaaa tcctgcttct tctaatgcct gtaggatatc cgccaaattt
    27661 tttttcaata attcttttcc cgaggtgggg ttataaatta cgcgtgcttt tttcattttc
    27721 agcctctatc gtttcgctaa ttcatcttta agtagcttat tcacgacacc aggatttgct
    27781 tgtcctttag ttgctttcat gatttgaccg actaggaagc caaccgcacg atcttttcca
    27841 tttttgaaat catcgacaga ttgttggttg ttatccaaaa tttcattaat gattggcagt
    27901 agtttgttcg gatcagataa ttgcacccaa ccattttttt caactacttc ttgggcttct
    27961 ccgccatttg tgatcaattc acggaagact tttttggcga tttttgagct gatcgttcca
    28021 tcggcgatca aacgaatcat tcctgctaag ttctcaggtg tcaattttgt ctcagctaat
    28081 tccagtttct cactgttcaa gtaagcagaa acttcaccca tcaaccagtt agatgcttgc
    28141 ttggcatctg caccattttc caaaacagct tcaaaaaaat cagacatttc ttttgtaagt
    28201 gtcaatacca ttgcgtcata ctctggaagt ccaaactcac ggatatagcg ttcgcgacga
    28261 gaagctggca tctcaggcaa actagctttt actttttgaa tccattcgtc atcgatcacg
    28321 aatttcggga tatcaggctc tgggaagtaa cgataatcac tagacccttc tttgacacgc
    28381 atcaaaactg ttttattcgt tgtttcgtca aaacggcgtg tttcttgttg gatttctcct
    28441 cctgaaagaa gaacttttgc ttgacgtttt tcttcaaaag ctaaaccttt tttcacgaaa
    28501 gataaagagt tcaagttttt caattctgct ttcgtaccga actcttcttg tccataaggg
    28561 cgaagagaaa tattggcatc acagcgcata gagccttctt ccattttcac atcgctgact
    28621 tctgtaaatt ggataattga tcgcaatgct tctagataag cgtatgcttc ttctggtgaa
    28681 cgcatatcag cttcagaaac gatttcgatc aaaggcgtgc cttgtcggtt caaatcaacg
    28741 taagaataac cgccaatacc atgcatgttt tttccagcat cttcttctaa atggacacgt
    28801 tcgatccgga tttttttctt tttaccttct acttcgattt cgatccatcc gtcatgaccg
    28861 attggttgat caaattggga aatttgatat gcttttggat tatctgggta aaagtaattt
    28921 ttccggtcaa aatgagtatc tttagagatt tcacagttta atgccaaagc tgctttcatc
    28981 ccgaattcca gcgcagcttt attcattact ggcagcacgc ctggataacc ccagtcgatc
    29041 acgtttgtat tgctgtttgg ttctgcacca aaatgagctg gcgctggaga gaaaattttt
    29101 gagttggttt ttaactcgac gtggacttct agtccaatga ctgtttcaaa attcattcgt
    29161 ctttccctcc taaaatagct ggttgttgtt tatggaattc tgttgcttgt tcaaacgcat
    29221 atgccgcttg atacatcgtt ttttcatcaa aataattacc gatgatctgc aatccaactg
    29281 gtaatccttc tgataaacct gccggtacag acatacctgg aagtccagcc aagttgacag
    29341 ggatcgttaa gatgtcactc atgtacattg tgattggatc gttaatgttc tcaccgattc
    29401 cgaatgcaac tgttggtgaa gatggcccga tgatcaagtc ataatctgca aagacttttt
    29461 caaaatctcg tttgatcaat gtacggactt gtcctgcttt tttgaagtaa gcgtcataat
    29521 aacccgcgct taaggagaat gtacctaaca tgatacggcg ttttacttct tcaccgaatc
    29581 cttctgaacg tgagttgaca tatacatcat ctaatgattt cacattttca gagcgataac
    29641 cgtagcggat accatcgaat cgttgcaaat tggaacttgc ttctgatgag gcaatgatat
    29701 aataaacagc cacaccgtat tttgaatgag gcaagctgac ttcttccaca acagcaccta
    29761 atgaacggaa tgtttctgct gctttcaata cagcttcttt tacgccaggg gccacacctt
    29821 cgcctaagta ttctttaggt aaagcaatct tcattccttt gatgtcgcct gtcagtccct
    29881 ctgcaaaatc aggaactgaa acgcctgaag aagtaccgtc tttttcgtca taaccgctga
    29941 tagctgtcaa tgctaatgca ttatctttca ccgtacgggt cattggtccg atttggtcaa
    30001 gagatgaagc aaaggcaatc aaaccaaaac gtgagacacg gccataagta ggtttcatcc
    30061 ctacgatgcc gttaaaagct gctggttggc ggatacttcc cccagtatca ctacctaaag
    30121 aaactggaat ttgtccagct gcaactgctg ctgcagaacc accagaagag ccaccaggta
    30181 cttttgttgg gtcccaagca ttttttgttt tcttgaaata agaagtttct gtacttccac
    30241 ccatagcaaa ttcatccatg tttagcttac caacagggat catatctgct tgatagattt
    30301 tatccatgac cgttgcatca taaattggtt caaaattgta aaggatctta gatgcagcgg
    30361 ttgtcaagat atcttttgtt acgatattat ccttgatacc aattggaata ccagcaagga
    30421 cgttgctttc atcaatccct ttttcatcta aagcttttgc ctgttccaat gctttttctt
    30481 cgttcaatgt aatgaatgcc tcgacctcag gctctgtttc tttgattcgt tcaaatgttg
    30541 tttttgtcaa atcttgtaca gtgatttctt tcgatacaag catatgatgc agttcttcta
    30601 gagaacgatt gtgtaattct gtcattatgc gccagcctcc ccattatcga tgattgcggg
    30661 tactttgata aagccgtctt ctgtttccgg cacatttttc aataattcat cgcggttcca
    30721 tccagcttgc gcccgatctt gtcgcatcac gtttactgtt tcaaccacat tcgacgtaaa
    30781 tggaacacct tccgtatcta cttcttccag catttcaacc atatcgatga ttttacctaa
    30841 ttggtcggta aaacttgcaa gttcttcatc tgcgaacgca agttttgcta atttggcaac
    30901 gtgtttcact tgttcttcac taatcgccat actacccctt ctttctaaac gagttaaaaa
    30961 ctttgctctc tattttaaca caagagtgcc gtgtctgaaa ttgtttattg aaatttgtct
    31021 gaaaaaactc atttttcatt cttctactga gcgactttta gccaatgcat ctgccatttg
    31081 atcttctgtc caaacttcga ttcctaattc ttgtgctttg gtcagcttgc tgccagcatc
    31141 ttctcctgct accacgatat ccgttttttt cgatacgcta ccagtcactt tcccgccaag
    31201 attttcgatc gtctcttttg cttcttcacg agtaaatcgt gttagtttac cagtaaggac
    31261 tactgttttt tctttaaaag gagactcgac ttcttgcaat tgggcatttc gaagcccttt
    31321 gtattcgaaa ttgacgcctg cttgttttaa ttcattcatc aattcatgga cttcttcgtt
    31381 actgaaataa gtgactacac tgtcggcaat gatatcaccg atcgtatcaa gcgcaataat
    31441 cgactcatag tcacttttac tcaatgtttc tagatcaccg aaatgttctg ctaaaatttt
    31501 tgctgctttc gctccgacat gtcggattcc taacccgaaa atcaaccgct cgaccgaatt
    31561 atctttgctg ttgtcgattg ctgtcaaaat attatttgct gatttttctt ttattttgtc
    31621 caacgtcaat aactcttctt ctgtcaattt gtaaagatca gcaacatctg ctactagttt
    31681 tttatcatac atctgttcta gtacgcgcgg ccccaaccca tcgatattca tcgcatttct
    31741 tgaaacaaag tggttcaaac cttctttcat ttgagcaggg catttaggat tgatgcaccg
    31801 taaggctact tcttcatcca aatggacaag ttcacttcca caaactggac aatgtgtagg
    31861 caattggtat tcttcgctgt ctttaggtcg tttatctaac acgacttgcg aaacttctgg
    31921 gatgatgtct cccgctttat aaatcagaac tgtatctttc aagcggatat ccttcaactt
    31981 aatataatca ccgttatgca ggctggcacg gcttactgtc gttcctgcta ctcggactgg
    32041 tgtcatgata gctgttggcg ttactacccc tgttcgaccg actgtccatt cgatgttttc
    32101 gattagcgtt tcaacttctt ctggcggaaa cttgtaagct gctgcccatc tcggtgcttt
    32161 gatagtaaaa ccaagttgat cttgtagcga gaattcattc actttgatga cgattccatc
    32221 gatttcatag ggcaaatcca cacgtttgtc atgatattct tcaatgtatg accacacttc
    32281 atcaatcgaa tgacatagac gtttttcccg attcgtgtgg aatccaattt tttctagctc
    32341 ttctagcgca tcatactggg tttttgcttg cataggaccg aaatcagcta ctgtgtataa
    32401 aaaagtgtcc agatttcttt tcgctgtgat tttagaatct aactgacgca aagaaccagc
    32461 tgcggcgttc cgaggattcg caaagatatc ttttccctct gcttctctgt cttggttcaa
    32521 ttggacaaat gaccgttttg gcatgaaaca ttcgcctctt acttcgatgt tcattggttc
    32581 ttttaatttg attgggaccg accggacagt ttttaggttt tcagtgatgt tttctcctac
    32641 agttccatcc ccgcgagtcg cacctctcac gaaatttcca tcttcataac gcaaagaaac
    32701 ggaaagtcca tctattttta gttcacagca atagtcaact acacgtccca ctgctttttg
    32761 tacacgttga tcaaaagcta tcagctcttc tttgctgaaa acgtcgttca aactgtaaag
    32821 cggaatatca tgagtgactt tttcaaaacc ttctaatact tttccgccta ctcgttgagt
    32881 gggagaatca gacgtgatca aatcaggata ttgttcctcg attgctacta attctgcgta
    32941 ttctttatca taaacatagt cttccactgt tggtttatct tctacgtagt attcgcgaga
    33001 ccattggttc agtcttgtac gaagttcttc tgctcgttta gcagcttcgt caatcgtcat
    33061 ttcatgttcc atcatttacc ctccactact aagctttttc aattggcgca aaggcagcca
    33121 aaagacgttt tactccttga cttgggaaag ccacatctag ttctagatcc ttcgacgtcc
    33181 cgcttactcg gacgactgtt cctagacccc attttttgtg tttgacttta tctccaggtt
    33241 tccagctgtt attttcccct ccagtagcac ttttagaggg aacaaccgct gtctctggct
    33301 gtttatagct atatgctgtt tttttagcga atacaggcgt ttttttaggc gtaggacgca
    33361 tgccttcgat ttccagtaac tcctgatcga tttcttctac aaaacgagaa ggtcggttat
    33421 attgcgtccg tccatataat gttctggaga aagcatttgt taaatacaac gcttcttctg
    33481 ctcgcgtgat ccccacatat gcgagacggc gttcttcctc cagttcgctt tcttccatca
    33541 atgctcgaga taaaggaaaa accccctctt ctagaccaat caggaataca actggaaatt
    33601 ctaatccttt ggctgcatgc aaagtcatca atgtcacttg ggaagcatct tcttctaaat
    33661 tgtcaatgtc ggaaacaagt gccagatcat ttaagaagac cgttagtttt tcttctggtg
    33721 catcagcgtc ttcttcattt tgctgttcga attgtttgtc gaattcttgt gtgaccgtta
    33781 aaaattcctc caagttttcg atgcgtgctt gtgcttctaa tgtattttgg atttttaagt
    33841 cttctagata tccgctacga tccaaaacct ctttcgtcaa ttctgtcact gtcaagtatg
    33901 ggatcatctg agtgacttct tggatcatct ctccaaatgc acctaattgc tgtcctgctt
    33961 ttccaccgat atttgccaga tccacatttt gcgctgcttc aagcagtggc cattcatgaa
    34021 gagaagcaaa actgcgtagt ttttcgatag atcctggtcc gatgcctcgt ttgggcgaat
    34081 taacgatacg ctcaaaactg attgaatctt gaggatttac cagcacattc aaatacgcta
    34141 aaatatcttt gatttcttta cgatcgtaga acttgtgccc tccaaccatt ttataaggaa
    34201 tatttgcttt caacagagtt tcttccatta cacgagattg tgcgttcgtc cggtacaaga
    34261 tggcaaagtc gccgtaattc cgatgattgc ttcgaatctc ttcttgcatc cggtctacga
    34321 taaaacgggt ttcgtctcgt tcattatctg cacggtagta agtgatcttg tttccttctt
    34381 tgttttctgt ccacaagttt tttggttttc gattgctatt gttttcaatg acttggtttg
    34441 ctgcgctaag gatgtttttc gtggagcgat agttttgttc tagcaaaatt actgcagcat
    34501 ctggataatc tttttcaaaa tccaagatgt tctgcatatc tgcaccgcgc cagccgtaaa
    34561 tactttgatc tgcatctccc acgacgcaca ggttcctaaa tctacctgcc aataaattga
    34621 ctagcgtata ctgtgcgtga tttgtatctt ggtactcatc tacatgaata taatggaatt
    34681 tgttttgata atacgtgagt gaatcaggat gttcttcaaa taatcgaatc gtattcatga
    34741 tcaggtcgtc aaagtccata cattgattat attgaagctc tttttggtaa gcagcataac
    34801 attttgccgc aatctcttcg taataactgc cttgcatttt tgcaaaatcc tgaggggtct
    34861 gcaggctgtt ttttgcttga gagattgttc ctaatatact tcgaggatca tattttttcg
    34921 gatcaatgtt cagttctttt agtatccgtt tcatcaaggt cagctgttca gaagaatcga
    34981 tgattgtaaa gttccggtta tacccgataa aatcaacatc tcttcttaaa atacgcacac
    35041 acatcgagtg aaaagtcgat acccagacat cttcgcctcc agatgcgaga atcgcattga
    35101 cacgttcttt catttctttt gcagccttat tggtaaaagt aatcgctagg atgttccaag
    35161 gattgacttc tttttcctca atcaaatagg caatgcggtg tgtcagcact ctcgtctttc
    35221 cacttcctgc acctgccatt agcagtaatg gtccgtcagt gtgaagaaca gcttctttct
    35281 gacgggggtt catcccgttt aataattcag ctttggatga cattaacaac atcctttctt
    35341 ttatcaatct tgttaattat agcattttct tggctttcat taaactagct agttcaaaat
    35401 catttgtttc tttttatgga tttttccttg tcaattcgtt gatttcgcgc taaattaaaa
    35461 gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa
//
LOCUS       DAZKMZ010000016        34963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000016 DAZKMZ010000000
VERSION     DAZKMZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.016"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="ACG8M5_000966"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="ACG8M5_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0358238.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="ACG8M5_000967"
     CDS             complement(2021..2923)
                     /locus_tag="ACG8M5_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0358239.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="ACG8M5_000968"
     CDS             complement(2920..3612)
                     /locus_tag="ACG8M5_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0358240.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="ACG8M5_000969"
     CDS             complement(3655..4296)
                     /locus_tag="ACG8M5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0358241.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="ACG8M5_000970"
     CDS             complement(4334..4648)
                     /locus_tag="ACG8M5_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358242.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG8M5_000971"
     CDS             complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG8M5_000971"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0358243.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG8M5_000972"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG8M5_000972"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0358244.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="ACG8M5_000973"
     CDS             complement(5985..6917)
                     /locus_tag="ACG8M5_000973"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0358245.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="ACG8M5_000974"
     CDS             complement(6914..7690)
                     /locus_tag="ACG8M5_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0358246.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG8M5_000975"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG8M5_000975"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0358247.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /locus_tag="ACG8M5_000976"
     CDS             complement(10934..12010)
                     /locus_tag="ACG8M5_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0358248.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="ACG8M5_000977"
     CDS             complement(12015..13295)
                     /locus_tag="ACG8M5_000977"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0358249.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="ACG8M5_000978"
     CDS             complement(13314..14240)
                     /locus_tag="ACG8M5_000978"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0358250.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="ACG8M5_000979"
     CDS             complement(14253..15536)
                     /locus_tag="ACG8M5_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0358251.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG8M5_000980"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG8M5_000980"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0358252.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="ACG8M5_000981"
     CDS             complement(16324..17235)
                     /locus_tag="ACG8M5_000981"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0358253.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG8M5_000982"
     CDS             complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG8M5_000982"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0358254.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="ACG8M5_000983"
     CDS             complement(17701..18399)
                     /locus_tag="ACG8M5_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0358255.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="ACG8M5_000984"
     CDS             complement(18386..18772)
                     /locus_tag="ACG8M5_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358256.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="ACG8M5_000985"
     CDS             complement(18919..20589)
                     /locus_tag="ACG8M5_000985"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0358257.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="ACG8M5_000986"
     CDS             20772..21005
                     /locus_tag="ACG8M5_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0358258.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="ACG8M5_000987"
     CDS             complement(21104..21535)
                     /locus_tag="ACG8M5_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0358259.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="ACG8M5_000988"
     CDS             21614..22027
                     /locus_tag="ACG8M5_000988"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0358260.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="ACG8M5_000989"
     CDS             complement(22065..23213)
                     /locus_tag="ACG8M5_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0358261.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="ACG8M5_000990"
     CDS             complement(23335..23931)
                     /locus_tag="ACG8M5_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0358262.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG8M5_000991"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG8M5_000991"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0358263.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="ACG8M5_000992"
     CDS             27161..27592
                     /locus_tag="ACG8M5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358264.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="ACG8M5_000993"
     CDS             complement(27637..28986)
                     /locus_tag="ACG8M5_000993"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0358265.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="ACG8M5_000994"
     CDS             complement(29056..30219)
                     /locus_tag="ACG8M5_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0358266.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="ACG8M5_000995"
     CDS             30348..30671
                     /locus_tag="ACG8M5_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358267.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="ACG8M5_000996"
     CDS             complement(30854..32203)
                     /locus_tag="ACG8M5_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0358268.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="ACG8M5_000997"
     CDS             complement(32272..33225)
                     /locus_tag="ACG8M5_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0358269.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="ACG8M5_000998"
     CDS             complement(33327..34517)
                     /locus_tag="ACG8M5_000998"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0358270.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34717..>34963)
                     /locus_tag="ACG8M5_000999"
     CDS             complement(34717..>34963)
                     /locus_tag="ACG8M5_000999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358271.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9952 a         7642 c         6120 g        11249 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga ata
//
LOCUS       DAZKMZ010000017        34501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000017 DAZKMZ010000000
VERSION     DAZKMZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.017"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(236..2128)
                     /locus_tag="ACG8M5_001000"
     CDS             complement(236..2128)
                     /locus_tag="ACG8M5_001000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358272.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2125..3852)
                     /locus_tag="ACG8M5_001001"
     CDS             complement(2125..3852)
                     /locus_tag="ACG8M5_001001"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358273.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3868..4518)
                     /locus_tag="ACG8M5_001002"
     CDS             complement(3868..4518)
                     /locus_tag="ACG8M5_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0358274.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4722..5018
                     /locus_tag="ACG8M5_001003"
     CDS             4722..5018
                     /locus_tag="ACG8M5_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0358275.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5190..5810
                     /locus_tag="ACG8M5_001004"
     CDS             5190..5810
                     /locus_tag="ACG8M5_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0358276.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5902..7068)
                     /locus_tag="ACG8M5_001005"
     CDS             complement(5902..7068)
                     /locus_tag="ACG8M5_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0358277.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7080..7433)
                     /locus_tag="ACG8M5_001006"
     CDS             complement(7080..7433)
                     /locus_tag="ACG8M5_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0358278.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7561..8583
                     /locus_tag="ACG8M5_001007"
     CDS             7561..8583
                     /locus_tag="ACG8M5_001007"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0358279.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8602..8763
                     /locus_tag="ACG8M5_001008"
     CDS             8602..8763
                     /locus_tag="ACG8M5_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358280.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9022..9456)
                     /locus_tag="ACG8M5_001009"
     CDS             complement(9022..9456)
                     /locus_tag="ACG8M5_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358281.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9566..11425)
                     /locus_tag="ACG8M5_001010"
     CDS             complement(9566..11425)
                     /locus_tag="ACG8M5_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0358282.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11426..11566
                     /locus_tag="ACG8M5_001011"
     CDS             11426..11566
                     /locus_tag="ACG8M5_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0358283.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11543..12703)
                     /locus_tag="ACG8M5_001012"
     CDS             complement(11543..12703)
                     /locus_tag="ACG8M5_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0358284.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13010..14434)
                     /gene="araA"
                     /locus_tag="ACG8M5_001013"
     CDS             complement(13010..14434)
                     /gene="araA"
                     /locus_tag="ACG8M5_001013"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0358285.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14450..15148)
                     /locus_tag="ACG8M5_001014"
     CDS             complement(14450..15148)
                     /locus_tag="ACG8M5_001014"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0358286.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15105..16766)
                     /locus_tag="ACG8M5_001015"
     CDS             complement(15105..16766)
                     /locus_tag="ACG8M5_001015"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0358287.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16984..18384
                     /locus_tag="ACG8M5_001016"
     CDS             16984..18384
                     /locus_tag="ACG8M5_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0358288.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18636..19715)
                     /locus_tag="ACG8M5_001017"
     CDS             complement(18636..19715)
                     /locus_tag="ACG8M5_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0358289.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19801..20760)
                     /locus_tag="ACG8M5_001018"
     CDS             complement(19801..20760)
                     /locus_tag="ACG8M5_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0358290.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20772..22283)
                     /locus_tag="ACG8M5_001019"
     CDS             complement(20772..22283)
                     /locus_tag="ACG8M5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0358291.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22305..22868)
                     /locus_tag="ACG8M5_001020"
     CDS             complement(22305..22868)
                     /locus_tag="ACG8M5_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0358292.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22874..23737)
                     /locus_tag="ACG8M5_001021"
     CDS             complement(22874..23737)
                     /locus_tag="ACG8M5_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358293.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23751..24647)
                     /locus_tag="ACG8M5_001022"
     CDS             complement(23751..24647)
                     /locus_tag="ACG8M5_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358294.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24822..26111)
                     /locus_tag="ACG8M5_001023"
     CDS             complement(24822..26111)
                     /locus_tag="ACG8M5_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358295.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26083..27600)
                     /locus_tag="ACG8M5_001024"
     CDS             complement(26083..27600)
                     /locus_tag="ACG8M5_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0358296.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27798..28688
                     /locus_tag="ACG8M5_001025"
     CDS             27798..28688
                     /locus_tag="ACG8M5_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0358297.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28761..29585)
                     /gene="nadE"
                     /locus_tag="ACG8M5_001026"
     CDS             complement(28761..29585)
                     /gene="nadE"
                     /locus_tag="ACG8M5_001026"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0358298.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29597..31069)
                     /locus_tag="ACG8M5_001027"
     CDS             complement(29597..31069)
                     /locus_tag="ACG8M5_001027"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0358299.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31706..32068)
                     /locus_tag="ACG8M5_001028"
     CDS             complement(31706..32068)
                     /locus_tag="ACG8M5_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0358300.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32291..33043
                     /locus_tag="ACG8M5_001029"
     CDS             32291..33043
                     /locus_tag="ACG8M5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0358301.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33183..34106)
                     /locus_tag="ACG8M5_001030"
     CDS             complement(33183..34106)
                     /locus_tag="ACG8M5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358302.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34121..34393)
                     /locus_tag="ACG8M5_001031"
     CDS             complement(34121..34393)
                     /locus_tag="ACG8M5_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358303.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10179 a         7194 c         5947 g        11181 t
ORIGIN      
        1 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtaaa
       61 atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc tcatattttg
      121 ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg
      181 actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg
      241 aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca
      301 tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt
      361 ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata
      421 ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt
      481 catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac
      541 gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca
      601 atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg
      661 ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat
      721 cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta
      781 caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca
      841 gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat
      901 ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa
      961 aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct
     1021 cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt
     1081 tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg
     1141 cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga
     1201 cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa
     1261 attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt
     1321 ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt
     1381 ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg
     1441 ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt
     1501 gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa
     1561 attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag
     1621 acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg
     1681 cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt
     1741 gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct
     1801 gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt
     1861 gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg
     1921 tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca
     1981 tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt
     2041 caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat
     2101 gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac
     2161 gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag
     2221 ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc
     2281 aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc
     2341 cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac
     2401 taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg
     2461 ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat
     2521 ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc
     2581 tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa
     2641 attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat
     2701 gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat
     2761 gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc
     2821 tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa
     2881 agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt
     2941 aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat
     3001 aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa
     3061 agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat
     3121 gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt
     3181 tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc
     3241 tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt
     3301 cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat
     3361 cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag
     3421 tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt
     3481 aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata
     3541 tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg
     3601 cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat
     3661 actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc
     3721 ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag
     3781 tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac
     3841 gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag
     3901 tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa
     3961 gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc
     4021 caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt
     4081 tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg
     4141 agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc
     4201 attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa
     4261 tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat
     4321 tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc
     4381 tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct
     4441 agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa
     4501 aaatgtctgt gtcggcatat ggatcctcct ttctttcaag atgacacatt gtcatatgac
     4561 gctgtgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt
     4621 atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt
     4681 gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg
     4741 tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata
     4801 ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc
     4861 caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca
     4921 ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca
     4981 ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa
     5041 gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt
     5101 tattcttcaa ccttatcaat atgacttgat agccttttct ttttctcata gttataacaa
     5161 tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc aaaggtcatc
     5221 ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta acaagttata
     5281 aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat gatattccgg
     5341 aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca gaattcactt
     5401 ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact gatcagttcc
     5461 ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc ccaacaaaat
     5521 taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat acagaaaacg
     5581 ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat ggcggtgtat
     5641 acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt gtaggcatcg
     5701 atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt gcagatgagt
     5761 tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa aaattgagat
     5821 aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgta ccttggatat
     5881 tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt cttcataaag
     5941 agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag ctgctgcata
     6001 aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag tcagcagaac
     6061 taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg gagagatcaa
     6121 gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga tcatgaatgg
     6181 acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc ctaagtaagg
     6241 gatcaggttc aaaaaacccg caataatccc caacgtgaca gcatactcta aaccaatgac
     6301 cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt gtcctcttat
     6361 ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt tttgcatgcg
     6421 tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca atagataaaa
     6481 taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc cgacaaaact
     6541 tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg ccgaattcat
     6601 gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg ggtcccgcaa
     6661 aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac tgatgaagct
     6721 cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga ttagtgcaac
     6781 aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct tttcaaagta
     6841 atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag gcagacctac
     6901 gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa ataaaaagac
     6961 gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat ttaaaaacca
     7021 gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa ttcctcctgt
     7081 tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg atgaagatag
     7141 attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga tacgtgagct
     7201 actgtctcta aattcccatt ggccatcgga ccgacataat cgatcgtata tacctgatca
     7261 tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga tacttcttga
     7321 atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat cagcatgggt
     7381 tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat cattgcactc
     7441 tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc cgtgtggaat
     7501 aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga atcagctcat
     7561 atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg aatctatacg
     7621 atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt tgaagaaaat
     7681 agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac tagtgtggat
     7741 ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc ggtaacagag
     7801 tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact atacggggcg
     7861 aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga actgaaagca
     7921 gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga tcatgcgcat
     7981 gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct tggtacagat
     8041 cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac taccttcact
     8101 gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca attcgcttat
     8161 ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga aacaggtgct
     8221 tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga aaatggaggg
     8281 gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg aggacataac
     8341 tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa gcaaatcatc
     8401 tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa ttttgtcgtg
     8461 tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga aacaggatta
     8521 ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta ttttgatcgt
     8581 tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact acgggaagaa
     8641 ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga agaggaatat
     8701 cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa tgtagaaaac
     8761 taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc gtctatcctg
     8821 atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga aaaggagtat
     8881 ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca ttatcataaa
     8941 ggattttgat cactctatca taaaggtaat attacattta tattagataa ctctagtttt
     9001 tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt tttggatggc
     9061 agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga atagatcttc
     9121 atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg ctacatatga
     9181 aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg gacgcaaagt
     9241 actgactttc ttaagcattg cccgatattt ttttgttcct tcttcaaata acaggatgat
     9301 ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg cgtttctgat
     9361 cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga cgatttgatc
     9421 gatggactgc acataatagc caatattcgg taacatgttc gttttcctac tttctccatt
     9481 gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg aaaaaattta
     9541 atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct gctaaactat
     9601 attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa atctcttgtt
     9661 gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac agttctcgtt
     9721 caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca tttgttgtct
     9781 gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt gcatcgtgag
     9841 tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt tcatgcaatg
     9901 tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga tgatcccccc
     9961 aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac tgcttcagtg
    10021 ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc ccacttccat
    10081 ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc atatgtgtct
    10141 gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt gtcattactt
    10201 ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc gtataggtca
    10261 tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc tctcttttat
    10321 acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg tttaacgctg
    10381 aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg gtggtgagtt
    10441 gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata tttgctgttc
    10501 caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct acttcataat
    10561 aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga tctgagaaac
    10621 tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta tttttttcat
    10681 cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa tatccttcag
    10741 gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa ccagtgttat
    10801 agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat gcttttcgca
    10861 acaaattatc gggattgttg aaattactga tataccctag cagtccaatt gccacaagca
    10921 gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga aacatgtacc
    10981 agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac agcatagtgc
    11041 cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct ggataaatcg
    11101 gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg atggctacga
    11161 catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa ataagaataa
    11221 tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa gcaaatttca
    11281 acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta ctaaaaacaa
    11341 caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc tgtttggtcg
    11401 aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa taaaagaatg
    11461 ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag aagaattaat
    11521 gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag cggacctttc
    11581 gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca aaatcagtac
    11641 tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat atatctgcga
    11701 tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg gttttctttt
    11761 gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat ggataattga
    11821 ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact gccaaacgtc
    11881 cgctgtctgc caaagcttcc tgagtcgatc cccctagatg aggcaacata agtatacggt
    11941 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
    12001 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
    12061 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
    12121 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
    12181 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
    12241 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
    12301 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
    12361 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
    12421 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
    12481 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
    12541 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
    12601 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
    12661 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
    12721 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
    12781 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
    12841 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
    12901 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
    12961 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
    13021 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
    13081 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
    13141 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
    13201 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
    13261 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
    13321 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
    13381 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
    13441 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
    13501 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
    13561 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
    13621 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
    13681 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
    13741 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
    13801 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
    13861 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
    13921 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
    13981 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
    14041 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
    14101 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
    14161 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
    14221 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
    14281 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
    14341 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
    14401 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
    14461 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
    14521 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
    14581 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
    14641 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
    14701 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
    14761 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
    14821 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
    14881 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
    14941 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
    15001 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
    15061 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
    15121 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
    15181 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
    15241 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
    15301 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
    15361 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
    15421 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
    15481 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
    15541 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
    15601 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
    15661 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
    15721 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
    15781 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
    15841 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    15901 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc gtttggctac actgttcgta
    15961 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    16021 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    16081 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    16141 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    16201 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    16261 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    16321 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    16381 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    16441 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    16501 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    16561 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    16621 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    16681 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    16741 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    16801 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    16861 aagcggttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    16921 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    16981 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    17041 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    17101 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    17161 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    17221 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    17281 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    17341 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    17401 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    17461 tatttgctaa aagtgttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    17521 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    17581 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    17641 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    17701 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    17761 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    17821 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    17881 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    17941 actttattga aaataggcgg tagcgtgatg ggactttcat tcatcctacc agctgtttta
    18001 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    18061 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcatagg cgaaatattc
    18121 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    18181 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    18241 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    18301 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    18361 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    18421 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    18481 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    18541 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    18601 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    18661 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    18721 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    18781 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    18841 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    18901 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    18961 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    19021 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    19081 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    19141 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    19201 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    19261 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    19321 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    19381 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    19441 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    19501 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    19561 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    19621 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    19681 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    19741 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    19801 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    19861 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    19921 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    19981 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    20041 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    20101 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    20161 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    20221 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    20281 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    20341 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    20401 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    20461 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    20521 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    20581 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    20641 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    20701 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    20761 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    20821 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    20881 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    20941 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    21001 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    21061 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    21121 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    21181 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    21241 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    21301 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    21361 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    21421 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    21481 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    21541 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    21601 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    21661 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    21721 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    21781 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    21841 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    21901 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    21961 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    22021 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    22081 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    22141 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    22201 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    22261 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    22321 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    22381 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    22441 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    22501 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    22561 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    22621 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    22681 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    22741 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    22801 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaaattt agtctcaact
    22861 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    22921 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    22981 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    23041 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    23101 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    23161 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    23221 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    23281 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    23341 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    23401 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    23461 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    23521 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    23581 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    23641 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    23701 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    23761 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    23821 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    23881 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    23941 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    24001 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    24061 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    24121 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    24181 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    24241 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    24301 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    24361 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    24421 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    24481 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    24541 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    24601 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    24661 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    24721 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    24781 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    24841 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    24901 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    24961 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    25021 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    25081 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    25141 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    25201 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    25261 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    25321 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    25381 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    25441 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    25501 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    25561 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    25621 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    25681 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    25741 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    25801 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    25861 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    25921 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    25981 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    26041 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    26101 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    26161 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    26221 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    26281 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    26341 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    26401 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    26461 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    26521 tgtttctgtc ctgattggtg caataacgtt taccaattga gcaagacaag caatctttac
    26581 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    26641 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    26701 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    26761 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    26821 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    26881 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    26941 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    27001 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    27061 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    27121 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    27181 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    27241 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    27301 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    27361 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    27421 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    27481 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    27541 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    27601 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    27661 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    27721 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    27781 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    27841 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    27901 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    27961 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    28021 ttcgtgtcga taaaatcgat attagttttc cagaaaaaat attcaatgct tttgatacga
    28081 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    28141 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    28201 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    28261 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    28321 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    28381 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    28441 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    28501 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    28561 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    28621 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    28681 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    28741 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    28801 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    28861 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    28921 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    28981 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    29041 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    29101 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    29161 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    29221 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    29281 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    29341 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    29401 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    29461 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    29521 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    29581 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    29641 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    29701 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    29761 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    29821 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    29881 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    29941 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    30001 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    30061 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    30121 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    30181 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    30241 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    30301 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    30361 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    30421 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    30481 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    30541 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    30601 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    30661 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    30721 cttggatcaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    30781 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    30841 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    30901 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    30961 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    31021 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    31081 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    31141 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    31201 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    31261 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    31321 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    31381 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    31441 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    31501 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    31561 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    31621 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    31681 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    31741 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    31801 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    31861 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    31921 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    31981 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    32041 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    32101 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    32161 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcatgagaaa
    32221 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    32281 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    32341 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    32401 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    32461 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    32521 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    32581 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    32641 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    32701 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    32761 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    32821 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    32881 taagaagtgg ttatcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    32941 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    33001 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    33061 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    33121 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    33181 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    33241 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    33301 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    33361 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    33421 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    33481 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    33541 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    33601 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    33661 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    33721 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    33781 tcaagatacc ttcgctcttt ttctttctga tagcccgtcg gtaaaagtgt cactttgtaa
    33841 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    33901 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    33961 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    34021 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    34081 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    34141 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    34201 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    34261 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    34321 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    34381 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    34441 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    34501 g
//
LOCUS       DAZKMZ010000018        34349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000018 DAZKMZ010000000
VERSION     DAZKMZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.018"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..942
                     /locus_tag="ACG8M5_001032"
     CDS             253..942
                     /locus_tag="ACG8M5_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0358304.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            929..2137
                     /locus_tag="ACG8M5_001033"
     CDS             929..2137
                     /locus_tag="ACG8M5_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0358305.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2431..3702
                     /gene="serS"
                     /locus_tag="ACG8M5_001034"
     CDS             2431..3702
                     /gene="serS"
                     /locus_tag="ACG8M5_001034"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0358306.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4251..5735)
                     /gene="guaB"
                     /locus_tag="ACG8M5_001035"
     CDS             complement(4251..5735)
                     /gene="guaB"
                     /locus_tag="ACG8M5_001035"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0358307.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6240..6935)
                     /locus_tag="ACG8M5_001036"
     CDS             complement(6240..6935)
                     /locus_tag="ACG8M5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0358308.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7025..8125)
                     /gene="ychF"
                     /locus_tag="ACG8M5_001037"
     CDS             complement(7025..8125)
                     /gene="ychF"
                     /locus_tag="ACG8M5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0358309.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8134..8325)
                     /locus_tag="ACG8M5_001038"
     CDS             complement(8134..8325)
                     /locus_tag="ACG8M5_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0358310.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8346..9236)
                     /locus_tag="ACG8M5_001039"
     CDS             complement(8346..9236)
                     /locus_tag="ACG8M5_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0358311.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9223..9990)
                     /locus_tag="ACG8M5_001040"
     CDS             complement(9223..9990)
                     /locus_tag="ACG8M5_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0358312.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10002..10715)
                     /gene="rsmG"
                     /locus_tag="ACG8M5_001041"
     CDS             complement(10002..10715)
                     /gene="rsmG"
                     /locus_tag="ACG8M5_001041"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0358313.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10947..12848)
                     /gene="mnmG"
                     /locus_tag="ACG8M5_001042"
     CDS             complement(10947..12848)
                     /gene="mnmG"
                     /locus_tag="ACG8M5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0358314.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12865..14262)
                     /gene="mnmE"
                     /locus_tag="ACG8M5_001043"
     CDS             complement(12865..14262)
                     /gene="mnmE"
                     /locus_tag="ACG8M5_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0358315.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14561..14944
                     /locus_tag="ACG8M5_001044"
     CDS             14561..14944
                     /locus_tag="ACG8M5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358316.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15112..15870)
                     /gene="jag"
                     /locus_tag="ACG8M5_001045"
     CDS             complement(15112..15870)
                     /gene="jag"
                     /locus_tag="ACG8M5_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0358317.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15888..16706)
                     /locus_tag="ACG8M5_001046"
     CDS             complement(15888..16706)
                     /locus_tag="ACG8M5_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0358318.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16703..17059)
                     /gene="rnpA"
                     /locus_tag="ACG8M5_001047"
     CDS             complement(16703..17059)
                     /gene="rnpA"
                     /locus_tag="ACG8M5_001047"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0358319.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17177..17311)
                     /gene="rpmH"
                     /locus_tag="ACG8M5_001048"
     CDS             complement(17177..17311)
                     /gene="rpmH"
                     /locus_tag="ACG8M5_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0358320.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17834..19168
                     /gene="dnaA"
                     /locus_tag="ACG8M5_001049"
     CDS             17834..19168
                     /gene="dnaA"
                     /locus_tag="ACG8M5_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0358321.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19366..20496
                     /gene="dnaN"
                     /locus_tag="ACG8M5_001050"
     CDS             19366..20496
                     /gene="dnaN"
                     /locus_tag="ACG8M5_001050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0358322.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20725..20967
                     /gene="yaaA"
                     /locus_tag="ACG8M5_001051"
     CDS             20725..20967
                     /gene="yaaA"
                     /locus_tag="ACG8M5_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0358323.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            20954..22078
                     /gene="recF"
                     /locus_tag="ACG8M5_001052"
     CDS             20954..22078
                     /gene="recF"
                     /locus_tag="ACG8M5_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0358324.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22075..24021
                     /gene="gyrB"
                     /locus_tag="ACG8M5_001053"
     CDS             22075..24021
                     /gene="gyrB"
                     /locus_tag="ACG8M5_001053"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0358325.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24041..26512
                     /gene="gyrA"
                     /locus_tag="ACG8M5_001054"
     CDS             24041..26512
                     /gene="gyrA"
                     /locus_tag="ACG8M5_001054"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0358326.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26748..27047
                     /gene="rpsF"
                     /locus_tag="ACG8M5_001055"
     CDS             26748..27047
                     /gene="rpsF"
                     /locus_tag="ACG8M5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0358327.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27097..27609
                     /gene="ssb"
                     /locus_tag="ACG8M5_001056"
     CDS             27097..27609
                     /gene="ssb"
                     /locus_tag="ACG8M5_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0358328.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27635..27871
                     /gene="rpsR"
                     /locus_tag="ACG8M5_001057"
     CDS             27635..27871
                     /gene="rpsR"
                     /locus_tag="ACG8M5_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0358329.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28014..29987
                     /locus_tag="ACG8M5_001058"
     CDS             28014..29987
                     /locus_tag="ACG8M5_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0358330.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            29993..30445
                     /gene="rplI"
                     /locus_tag="ACG8M5_001059"
     CDS             29993..30445
                     /gene="rplI"
                     /locus_tag="ACG8M5_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0358331.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30719..32086
                     /gene="dnaB"
                     /locus_tag="ACG8M5_001060"
     CDS             30719..32086
                     /gene="dnaB"
                     /locus_tag="ACG8M5_001060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0358332.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32866..34158
                     /locus_tag="ACG8M5_001061"
     CDS             32866..34158
                     /locus_tag="ACG8M5_001061"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0358333.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10473 a         6689 c         6812 g        10375 t
ORIGIN      
        1 agaaaatgct tatttatcaa catttctagc ttacatgatc attggttagt tctggtcctt
       61 tttcgttcgt ctttataact ccgagagacg taaaatcctt ttgttttcag tttaatgttc
      121 atgttcttgt cttattcaca ccaactagat gtctcacctt ctaaaatttt ccttaacaaa
      181 gtgctgcttt tatctatttc tgcttttttt tgctagaata atagtaacaa tatgactctg
      241 gaggatttat ttatgaaaat tttgattgct gacgatgata aagaaatcgt tgaattatta
      301 agtatctatg ttcacaatga ggggtatgaa gcagtaaagg cttatgatgg gaaagaagca
      361 ttatctaaaa tcagaacaat ccctgatatt gaattactta ttttagatat catgatgcct
      421 gagatggatg ggatgcaagt agtgaaagag ctacgaaaag aatcccaaat accaatcatt
      481 atgctgacag caaaaactac tgatatggat aagatcaaag gactggtcgc tggtgcagat
      541 gattatgtga ccaagccatt taatccgtta gaagtaatgg ctagagtaaa gtctttatta
      601 cggcgtacaa aaatgcagat tgccccagat cagcctgatg tattagaagt aaatgcatta
      661 atcatcaata aagactctca tgaagttaaa acaatcgatg ggaaagaaat ccagttaaca
      721 gctttggaat tcggtatcct ctacttgctt gctagtcatc ctaatcgagt atttagcgcg
      781 gatgaaattt ttgagcgtgt ttggaaacaa gaaagtattg tctctgctaa aactgtcatg
      841 gttcacgtta gtcatttaag agataagatt gaagaagcaa ctggtggtga aaaagtcatt
      901 caaaccgttt ggggagtggg ctataaaatt gacgcaagat agtaaacgag caactgaaaa
      961 acgccgtcgg atcaatttga cttcaaaaga aatcagcgaa ttgttagcgg aaggaatcat
     1021 cacgattatc cttttgcttc tattaaatgt ttctattctg gttgttatca gttcggtgat
     1081 caacagttca ccttctttga cgaatgcgat ttgggattca aagaatattt ttgcagaacg
     1141 attgaacact gatttgttct ggaacggccg gaattttatt attccctttt tctttctatt
     1201 agatattggt gttttgtatt ggcgcttgat tcgaagatat cgccaaatgc aattgcggca
     1261 tatcatcagc gaacttcact acatcgcaaa cggaaattat gatcatcgta tcccttttga
     1321 actgagtggt gacttaagcc gagtggtaac aagtatcaat ggattggtgg atagtaccgt
     1381 agcagcgatt gaagacgaac gtaaaatcga aaaatcaaaa gatgaattga tcacgaatgt
     1441 cagtcatgat atccgtaccc cactcacgtc catcattgga tacctaggac tgatagaaga
     1501 tggccagtat cactcagaag aagatctttt aaagtatacg catactgctt atatcaaagc
     1561 aaagcagatg aaatctttag tggacgactt atttgaatac actaaggtac gccagcctgc
     1621 tgtcccggtg aatttttctg cctttgacat gatccagttg atcgaacagt tagcagctga
     1681 ttttgagttg gaagcttcta aaaaaaatat ccaaatactt gttcaatcaa aagtagactc
     1741 ccttatcatg gatggcgata ccgaaaaact tgtacgtgtc ttcaataatc ttttgacgaa
     1801 tgcgttgaaa tacggaaaag gagcaacgaa gatcgtaatc gaagtagaac gtatcggttc
     1861 agaagttgtt gcaacagtca aaaataacgg agcaatgatc ccacaacagg caatcgataa
     1921 tctattcgac cgtttttatc gtgtagaaga atctcgttca caagcaactg gaggaactgg
     1981 acttggttta gcaatcgctc aaagcattgt ggctttgcac ggcggataca tttatgcaaa
     2041 atcagataag caatggactt ctttcatcat gcaccttcca atcaagaaaa atgaaaaatt
     2101 acctattgat acacaggaag ttattgacga aagctgaaat attcgctatt cttattacaa
     2161 ttattagaat aaatgaaaaa cacagaaccc agtacacttt attctttttc agagagctga
     2221 tggcaggtgt aaatcagcaa aggataattt ggaagttcca gttcgtttac gtgccctttc
     2281 gaaataagaa aggtcggttc aacagccgtt atgtgttcaa caagtgcagc tgacgctgaa
     2341 cttgggtggt accgcgaatt tcagactgat tcgtcccaag aataatggga tggatcagtc
     2401 tttttttatt ttcaaaaagg aggaaatcat atgttagatg taaaaatgat gcgacaaaat
     2461 attgaagaag tacaagaaaa attgatgact cggggcgtaa aaaaagaaac attagctgaa
     2521 ttcatggaac tagatgaaaa tcgtcgtaag ctactagtca aaagtgaaga attaaaaaaa
     2581 tatcgaaatg atgtttctgc tgagatcgcc caattgaagc gtgcaaaaga agatgcttct
     2641 gctaaaatcg ctgaaatgaa agaagtcggt ggcaacatca aagcattgga tactgaaatt
     2701 gctgaagtgg atgaaaaatt gaaacaaatt gcaactactt tgccaaacct tccccatgat
     2761 tctgtgccag ttggaacaga tgaagaggac aatgtagaaa tccgtcgttg gagcgaacca
     2821 cgtacatttg cttttgaacc aaaaccacac tgggaaattg cagaaaatct tgatatttta
     2881 gattttgaac gtggcgcaaa agtagccggt agccgttttg tctattacaa aggattaggt
     2941 gctcgattag aacgtgctgt ctacaacttc atgcttgatc agcatattta cgaacacggc
     3001 tatactgaaa tgatcactcc ttatatcgtc aacagtaaag cgatgtttgg tacgggacaa
     3061 tttccaaaat tcaaagaaga tgttttccaa ttggagaata ctgatctaac attaatccca
     3121 acagctgaag taccattaac taactattat aatgatgaaa ttttagatgg tgcagattta
     3181 cctatttatt tcactgcact aagccctgct tttcgttctg aagccggaag tgctggtcgg
     3241 gatacacgag gactgattcg tcttcaccaa ttcaataaag tagaaatggt caaattttcg
     3301 gatgtagagc attcttacga agaattggaa aaaatgacag ctgatgcaga agatatcttg
     3361 aaaaaattgc atttgcctta tcgtgtgatg gccttatcaa ctggagacat gggcttttct
     3421 gcagctaaaa cttatgactt agaagtatgg atacctgcac aagatactta tcgtgaaatc
     3481 agttcttgtt ctaactgtga agatttccaa gcacgccgcg caatgatccg ttatcgcgat
     3541 aatgatggaa aaatccacta tactcatact ctgaatggtt caggacttgc agttggccgt
     3601 acagtagctg ccattttgga aaactaccaa aatgaagatg gttctgtcac agttccagaa
     3661 gcgctcgttc cttatatggg gaatctgaaa gttattaaat aaagaggctg tgaaaaagcc
     3721 gcttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     3781 ttggacttaa taccgagaaa ctggcttttg aacaccattt atcgagaggt tgtgaaatca
     3841 gcgaattaac ctgagtatta gagaaaaaaa tggaaaaata gtccctcata ttttaccgtt
     3901 tgttgaacta ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtaagcc
     3961 taccccccag ctcctagtta caaggacaaa ttcaaaaaac agctcttcat attttatgaa
     4021 tgttgtactt gtgattcgga ggtggcaagg gaacactgtt tattcaaaaa gaggctggga
     4081 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
     4141 gtgaatttct tcttatttcc gaaggagttg cttctacttc cgccgtttat acgtgtttag
     4201 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttaatgttt ttaagattca
     4261 actgaatagt ttggtgcttc ttttgtgatt tgtacatcat gagggtgtga ttcttttaat
     4321 ccactgccgc tcatttggat aaattgcgct tcatcacgta attgctgtag attggctgcc
     4381 ccaacataac ccattcctga acgcaaaccg ccgatcattt ggaaaataat atctgcaaca
     4441 cttcctttat aagcaacccg accttcgatt ccttctggta ctaatttatt tgcttcattg
     4501 acactacctt ggaagtaacg gtcttttgaa cctttttcca tcgcgcctaa tgagcccata
     4561 cctcggtatg ttttgaatcg acgtccttgg aagatttcga attcacctgg tgattcatct
     4621 gtccctgcca acatacttcc tagcatcact gcatgtccgc cggcagctaa tgctttgacg
     4681 atgtcaccag aatatttgat ccctccgtct gcaataatcg ctttgccata ttgacgagca
     4741 actgatgctg catcataaat agctgtgagc tgaggtacac ctacaccggc taccacacgt
     4801 gtcgtacaaa tcgatcctgg tccgatacca acttttacga catctacacc tgcatcatat
     4861 aaagcttttg tcgcttcagc cgtcgcaacg tttcctgcaa tcaatgttgc atctgcaaaa
     4921 gttgaacgga tttcttgaat ttttcggatc acacctgcac tatgtccatg agctgtatcg
     4981 atgatgattg catctgcccc tgcttctaat aaagcattcg cacgctcaaa tgtatcgctt
     5041 gttacaccaa cggctgctgc tacaagcaat cgaccatgtt catcttttgc tgcatttgga
     5101 aattcgatca ctttctcgat gtctttgatc gtgatcaatc cgcttaaacg accttcgtta
     5161 tctacgattg gtaatttttc gattttatgt ttttgcaaaa tcttttctgc atcttttaga
     5221 gaggtaccta ctggggcagt cactaagttg tcttttgtca tcacatcagc aatcggcatg
     5281 tgataatctg ttacaaaacg catgtcgcgg tttgtaatta tccctaccaa ttttctgttt
     5341 tcaagtgttt caacgattgg cacaccactg atacgatatt tgctcatcaa ttcttctgcg
     5401 tctgctacaa gatgctgtgg cgttaagaag aacggatcaa tgattacgcc actttcagaa
     5461 cgtttgactt ttcttacttc atccgcttgc tggctgatcg tcatgttttt atggatcaca
     5521 cctagtccgc cttgtcttgc catagcaatg gccatcttgc tgtcggtaac agtatccata
     5581 cttgcactga tgattggaat attcaactta atatttttag ctaattgtac gctcatatcc
     5641 acttcgtttg gtaacacgtg actttctgct ggtatcaata agacatcatc aaaagtaaat
     5701 ccttttttcg tgaactttgt ttcccagttg gacattttta gtaccactcc tctaatttag
     5761 tttatctatt aaaataattg attttttcat ttaagtcaat gatctacttg gaatcaagtt
     5821 agctttctca tagaatacca atataaagcg tttacatctg tgtgtattaa aagagattgt
     5881 aaaaaagctg tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt
     5941 ttgaattggt tgggtttatt accgcagatg caaacttttg aacaccgttt agtggagagg
     6001 ctgtgaaaaa gctgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac
     6061 atttttgcat aaattggact taataccgag aaactggctt ttgaacaccg tttatcgtaa
     6121 aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact atggataaac
     6181 aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt tttgctagtt
     6241 tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa gtagtaacgg
     6301 attgctaaag cagttgcccc gataatgata actactactg gatctaatac tggattgata
     6361 actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt cgttccacct
     6421 aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc gggatattca
     6481 tattgataga tgtatttgta catcatataa aagacccagc caccgattgc agaacctaga
     6541 acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc ggtaatcgca
     6601 atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat caataaaggg
     6661 gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct ttcagagact
     6721 gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa agtaggcaga
     6781 atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc taaagatttt
     6841 ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa actattgttt
     6901 tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc cttagccgtt
     6961 ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta gtttgatagc
     7021 ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca taatcttttc
     7081 cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa tgatccaagt
     7141 cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa tggatgattc
     7201 ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact tcttgttccc
     7261 cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc aactgatcca
     7321 aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct tcatctaatt
     7381 ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt tcttctgcag
     7441 caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg tcatcttctg
     7501 agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat ttaacgattt
     7561 tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc agtacaggtt
     7621 tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt ttagcaactt
     7681 ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact agttccaagt
     7741 tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta atattatcat
     7801 catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg cttaagaatt
     7861 gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata tctgtaaatt
     7921 caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt tgcaaacgcc
     7981 agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga tagtttgctg
     8041 cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg ttgggcaacc
     8101 caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat ttttttcaat
     8161 tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat gtccgcaatt
     8221 catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat tcgtctggca
     8281 agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa tagcctcctt
     8341 tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt caaatctttt
     8401 tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat agctacactt
     8461 gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg tttttccttt
     8521 gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc agaaacaagc
     8581 tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa tttcaagatc
     8641 aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa acgctgatct
     8701 tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt ggcgatataa
     8761 ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag gtttttcatc
     8821 agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg caagttttcc
     8881 agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc agggatcgtt
     8941 tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat ctcatatcct
     9001 tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc aatagaatta
     9061 gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg atttggtcgc
     9121 agctcattca atggtagctg gacaacggta tcatctttta catccaccgt ctctaagttt
     9181 tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt cttcacgact
     9241 taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg gatcgtagtc
     9301 gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg gaatgatcgt
     9361 ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat ttcccagatt
     9421 tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg gattaaagtg
     9481 tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg cgtaatactc
     9541 acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg tcagatggcc
     9601 aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt ctgctaaaga
     9661 tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg ctcctgctaa
     9721 ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag ttgcctcatc
     9781 aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag gttttctgat
     9841 cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata cttttttgcc
     9901 aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga cgccaccttt
     9961 ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat tggctgctta
    10021 ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt ttgaataacg
    10081 atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt gaggaattta
    10141 ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact ttttgctgct
    10201 ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc cgctaacaca
    10261 ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa gtgaggattt
    10321 tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa taatcgggtt
    10381 aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat ctgcagttct
    10441 ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac atcgcataaa
    10501 gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta aaaatgcttc
    10561 agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt ccattcgact
    10621 aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc tatccctttt
    10681 tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat tgtgaaacag
    10741 caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat gcaatccttc
    10801 catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt ctttctagta
    10861 tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg caaaaactag
    10921 ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc aatcttccct
    10981 tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact gattcggcta
    11041 gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc tgttgctaat
    11101 ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat gcgttttaat
    11161 ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg gatttctact
    11221 tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag aagttctgca
    11281 taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt taatggtgcc
    11341 gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg tacattcatc
    11401 aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt gtattgatct
    11461 tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc attatcatga
    11521 cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc gttcgttcct
    11581 tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt gatcactaaa
    11641 ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc ttgtccagcc
    11701 gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt ttcaacgact
    11761 ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc gatagcatat
    11821 cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat gttgacttga
    11881 acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt atttaagcct
    11941 tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat cttatcttcg
    12001 atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat tggggcacga
    12061 tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag ccagcaagat
    12121 tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt cggctctttg
    12181 tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac tcgaggcggt
    12241 gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt cgctagtcca
    12301 atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat aatgatttct
    12361 ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct cgctcctgtg
    12421 gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac gattccttgt
    12481 cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga ataagcatgt
    12541 ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt caacatacgc
    12601 atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc atcgatttct
    12661 cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat gaatgctacc
    12721 atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc agcaagtgca
    12781 gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc tgcttcataa
    12841 tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga ataattgagt
    12901 gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc agcaacgcgt
    12961 catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa tgacttcttc
    13021 aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa cataagtagc
    13081 gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt ccaaacgatc
    13141 tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga tcaatttctt
    13201 caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa tgatccgttt
    13261 caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt cgctttggtt
    13321 tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt cgaccccgat
    13381 ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct tgagcggtac
    13441 cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa tatccgtaac
    13501 gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga catttggccg
    13561 gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg atgtagcaag
    13621 tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact tcgtagtcaa
    13681 ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg ctaatgtctc
    13741 taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta actgattaag
    13801 agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag cttctgcctg
    13861 tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg gctcagctaa
    13921 tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga tcccaccgtg
    13981 acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca tcacggagac
    14041 catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat aatggatcgt
    14101 atgacttggt acttctgata atcgtttgtt tccagattga taaactttat ctgctagttg
    14161 cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag gtggggtaga
    14221 aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac ttcctttttc
    14281 attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg gtgaacttcg
    14341 cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact tgctataagg
    14401 aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat taccttatat
    14461 ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa gatactgtat
    14521 aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc tatctaaact
    14581 atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat tggaagaaga
    14641 aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc agaaatatat
    14701 cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg acccatcttt
    14761 tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg tacttgaata
    14821 tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac gcgacggaaa
    14881 gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa caacgattgg
    14941 ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat ccgctttttt
    15001 ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc tagtaaaata
    15061 tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga atcaataata
    15121 ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg aatgtgtttt
    15181 tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg caggcattgg
    15241 ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat cagctaaacg
    15301 ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta cagacagctt
    15361 atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat tcagtgtttt
    15421 tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa caaccatatt
    15481 atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg aaatttcagt
    15541 aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta cttcttctgt
    15601 tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt cttcttccaa
    15661 aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac ttggctggat
    15721 cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt cttccagtat
    15781 ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt ggacagcttc
    15841 gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta tttccgtttt
    15901 ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct ccgtgcttct
    15961 tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg tacaacttgg
    16021 aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac acccatcact
    16081 aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga ttcaatttga
    16141 ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat cggcaaaata
    16201 aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt caactctggt
    16261 acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa taaaggaaga
    16321 caaccagcat atggattgac gccattttct gcatataaac gctgctgctc ttctcttaac
    16381 ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt taatttcggt
    16441 tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg caataggatc
    16501 acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa tgacaacgcc
    16561 ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt actctgtgcg
    16621 ctgacttccc cagttccgca accggataag acgatgacca aactaacgat accggccatc
    16681 aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt atttttgcaa
    16741 gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc ttcacacttg
    16801 gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata gaataaactg
    16861 ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc ttttttccta
    16921 cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa acgacgaatt
    16981 tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt tcttttttta
    17041 ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta cgctcataat
    17101 cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata aaaaaaacca
    17161 ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga cggctagcta
    17221 acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact ttttgacgtt
    17281 tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg ttcttatcta
    17341 ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt tgtcaatata
    17401 aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat gggaatccta
    17461 tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt tttacacaga
    17521 aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat tccgtctcct
    17581 aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta ttggttttta
    17641 cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact gtgtttttct
    17701 cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt tcttcggttt
    17761 ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt tttaatgaag
    17821 gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag caacatacca
    17881 aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc ctcgaaccct
    17941 tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat attgggaaaa
    18001 aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg agattatccc
    18061 tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa aaaatccaaa
    18121 agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata cctttgatac
    18181 gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg tagcagaaga
    18241 tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg gtaagactca
    18301 cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca aagtaaaata
    18361 tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca aaacagcaga
    18421 agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca ttcaattttt
    18481 tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct tgtacaatga
    18541 agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga aactgcaaga
    18601 acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc cggatcttga
    18661 aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga
    18721 cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag aaggtgcgct
    18781 tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta gtctagctgc
    18841 agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa tcctgcagat
    18901 ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag gaaagaaacg
    18961 cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg agctcacaga
    19021 taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa cggttatcca
    19081 tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa atgaagtatc
    19141 agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa aatagcatgt
    19201 ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg ttttttcgtt
    19261 tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact tttattattc
    19321 tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa agttacttta
    19381 aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc aagcaaaacc
    19441 acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt gactttgacg
    19501 gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga aaaagcaaat
    19561 atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag cgaaatcatt
    19621 cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca agtagcgatc
    19681 acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta tcctcatctt
    19741 cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac taaactaatc
    19801 aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac aggtgtgcat
    19861 ttcattttat ctgataattc tttattagct gtagctactg attctcaccg tctaagtcaa
    19921 cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc tggaaaaagt
    19981 ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat cagcattatg
    20041 gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt gctagaagga
    20101 aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt tgaattttct
    20161 gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga aggccgtaac
    20221 aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa ttcgccagaa
    20281 atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc attagatatt
    20341 tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa
    20401 gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga tggcgttcaa
    20461 tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat gttcttgaaa
    20521 atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta aaatctcttt
    20581 taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg tcttatatgg
    20641 aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt
    20701 aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg
    20761 acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg
    20821 tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa
    20881 ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa
    20941 ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga
    21001 gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa
    21061 aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag
    21121 tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa
    21181 aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat
    21241 caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt
    21301 tgcaccagaa gatctttctt tagtaaaagg cagcccgcag atcaggcgga agtttttaga
    21361 tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat
    21421 tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct
    21481 ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag
    21541 agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca
    21601 tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac
    21661 gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct
    21721 ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca
    21781 gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa
    21841 attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga
    21901 tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg
    21961 aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt
    22021 tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca
    22081 gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt
    22141 gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag
    22201 ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt
    22261 tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac
    22321 ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc
    22381 tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga
    22441 cttcacggag taggatcttc tgttgtaaac gcgctttcta cacagcttga tgtcaaagta
    22501 tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta
    22561 aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa
    22621 atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt
    22681 gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc
    22741 atcaatgaat accattatga cggcgggatc aaaagttatg tcgaatactt gaatgcaaac
    22801 aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt
    22861 gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat
    22921 atccatacgt atgaaggtgg aacacatgaa tcaggattca agactgcttt aactcgtgtg
    22981 ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt
    23041 gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc
    23101 gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta
    23161 tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt
    23221 gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg
    23281 agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc
    23341 aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct
    23401 aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat
    23461 gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact
    23521 gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta
    23581 gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt
    23641 ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct
    23701 ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa
    23761 gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt
    23821 tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc
    23881 aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa
    23941 atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat
    24001 gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga
    24061 aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat
    24121 gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg
    24181 ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc
    24241 tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagaca ttgcgatcta
    24301 tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca
    24361 tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg
    24421 gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca
    24481 aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct
    24541 tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa
    24601 cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa
    24661 tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatggggaa
    24721 atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt
    24781 tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat
    24841 ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga
    24901 agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat
    24961 ccgtcgtgac gccagcgcat ccgttatctt gaataactta tataaattga catccttgca
    25021 aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt
    25081 gaaacaaatc cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac gacggacggt
    25141 ctttgaaaaa gaaaaagcag aagcaagagc acacatctta gaaggtttgc gtattgcttt
    25201 ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa
    25261 agcaacaatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat
    25321 gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact
    25381 cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat
    25441 catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt
    25501 actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt
    25561 tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca
    25621 acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa
    25681 tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta
    25741 tcgggcaaaa ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa tcccaatcat
    25801 caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga
    25861 agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc
    25921 tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga
    25981 cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca
    26041 taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc
    26101 cggggtcaga gggatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa
    26161 ggaaaatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc
    26221 ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa
    26281 aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac
    26341 agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac
    26401 attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt
    26461 cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat aaccatcaag
    26521 actaagtgtg taagctagca agaataaccg attaaaaaat ctggttattc ctgctggctt
    26581 ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga
    26641 ccaaaataga gtgtataatt agagcttgtg agtagtctaa ttaattagat aactctcctt
    26701 gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga
    26761 aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa
    26821 tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt
    26881 gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg
    26941 ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa
    27001 tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg
    27061 tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt
    27121 ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca
    27181 tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt
    27241 atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga
    27301 acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa
    27361 cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca
    27421 tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt
    27481 ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac
    27541 cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta
    27601 ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg
    27661 taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga
    27721 tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac
    27781 tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat
    27841 gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct
    27901 tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct
    27961 cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa
    28021 ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag
    28081 tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga
    28141 atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg
    28201 aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca
    28261 tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat
    28321 tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt
    28381 ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg
    28441 atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa
    28501 aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag
    28561 atgccattga taaattagat gacaaagaga tttcttattt gaactctttc gtcactacac
    28621 tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt
    28681 ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc
    28741 tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga
    28801 ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg
    28861 ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac
    28921 ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag
    28981 cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc
    29041 acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct
    29101 tcaaccagaa aaaagcttac atcgttatca atgagaacga gatcattcca gatgtagaac
    29161 ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac
    29221 gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt
    29281 ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc
    29341 atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatattgaa tcttcagctt
    29401 cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct
    29461 tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca
    29521 atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag
    29581 acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca
    29641 atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg
    29701 aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca
    29761 atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca
    29821 gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact
    29881 tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat
    29941 ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt
    30001 aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc
    30061 aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag
    30121 tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga
    30181 agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc
    30241 gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg
    30301 gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat
    30361 ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat
    30421 caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata
    30481 accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc
    30541 aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc
    30601 ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg
    30661 atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat
    30721 gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt
    30781 aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa
    30841 agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg
    30901 taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga
    30961 agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa
    31021 tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac
    31081 agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga
    31141 tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc
    31201 tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga
    31261 agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca
    31321 gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt
    31381 gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga
    31441 aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag
    31501 ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg
    31561 gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat
    31621 ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga
    31681 ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat
    31741 ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca
    31801 gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga
    31861 atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta
    31921 tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga
    31981 agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa
    32041 agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga
    32101 gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact
    32161 agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc
    32221 tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa
    32281 aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta
    32341 cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    32401 atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg
    32461 ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc
    32521 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag
    32581 ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga
    32641 aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac
    32701 cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa
    32761 gcgtttttat aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat
    32821 tgatacaatg gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta
    32881 gtagtaggta cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa
    32941 aatgctgaag tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt
    33001 aacggtgtga cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc
    33061 agtgtgatcg gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat
    33121 ttacatgaaa aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc
    33181 ttgccttatc atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt
    33241 ggtacaacga ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc
    33301 cgtattgcag acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat
    33361 gacaaaaacc gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt
    33421 tttgaagaat attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt
    33481 atcttgaatg atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta
    33541 atgctggata tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt
    33601 ggggtaacaa tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc
    33661 aaagcctata cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca
    33721 ggagaacaaa tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt
    33781 gtaggctggt ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac
    33841 ctatctttaa actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct
    33901 tatgaattag atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc
    33961 tgcaaaccaa tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca
    34021 cttgcagaac tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt
    34081 gtacgtatct caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc
    34141 gtctgggcgc aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg
    34201 agtcgaagtg tttatctata cgataataat aacttgaaga ggtagtgaaa gagacgttta
    34261 gccttattgc cgataggctg tctcttgaac actgtttatt cagaataagg gactgtgaca
    34321 aaaaccgtgt cacagtccct ttttccgaa
//
LOCUS       DAZKMZ010000019        34118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000019 DAZKMZ010000000
VERSION     DAZKMZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.019"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8M5_001062"
     CDS             complement(99..1229)
                     /locus_tag="ACG8M5_001062"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0358334.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8M5_001063"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8M5_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0358335.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8M5_001064"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8M5_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0358336.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8M5_001065"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8M5_001065"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0358337.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8M5_001066"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8M5_001066"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358338.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8M5_001067"
     CDS             4880..5692
                     /locus_tag="ACG8M5_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358339.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8M5_001068"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8M5_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358340.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG8M5_001069"
     CDS             complement(6502..6732)
                     /locus_tag="ACG8M5_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358341.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG8M5_001070"
     CDS             complement(6716..6913)
                     /locus_tag="ACG8M5_001070"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0358342.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG8M5_001071"
     CDS             complement(7059..7937)
                     /locus_tag="ACG8M5_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0358343.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG8M5_001072"
     CDS             complement(7976..9094)
                     /locus_tag="ACG8M5_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0358344.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG8M5_001073"
     CDS             complement(9091..11190)
                     /locus_tag="ACG8M5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0358345.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG8M5_001074"
     CDS             complement(11241..12062)
                     /locus_tag="ACG8M5_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0358346.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG8M5_001075"
     CDS             complement(12059..12868)
                     /locus_tag="ACG8M5_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0358347.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG8M5_001076"
     CDS             complement(12889..13383)
                     /locus_tag="ACG8M5_001076"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0358348.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG8M5_001077"
     CDS             complement(13395..13895)
                     /locus_tag="ACG8M5_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0358349.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG8M5_001078"
     CDS             complement(13960..16656)
                     /locus_tag="ACG8M5_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0358350.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACG8M5_001079"
     CDS             complement(17084..18157)
                     /locus_tag="ACG8M5_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358351.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACG8M5_001080"
     CDS             complement(18154..18981)
                     /locus_tag="ACG8M5_001080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358352.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACG8M5_001081"
     CDS             complement(18982..19788)
                     /locus_tag="ACG8M5_001081"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358353.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACG8M5_001082"
     CDS             complement(19800..20885)
                     /locus_tag="ACG8M5_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358354.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACG8M5_001083"
     CDS             complement(20902..21444)
                     /locus_tag="ACG8M5_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0358355.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACG8M5_001084"
     CDS             21702..22691
                     /locus_tag="ACG8M5_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0358356.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACG8M5_001085"
     CDS             22688..23425
                     /locus_tag="ACG8M5_001085"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0358357.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8M5_001086"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACG8M5_001086"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0358358.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACG8M5_001087"
     CDS             23989..24621
                     /locus_tag="ACG8M5_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0358359.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACG8M5_001088"
     CDS             24706..25353
                     /locus_tag="ACG8M5_001088"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0358360.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACG8M5_001089"
     CDS             25358..26341
                     /locus_tag="ACG8M5_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0358361.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="ACG8M5_001090"
     CDS             complement(26744..28258)
                     /locus_tag="ACG8M5_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0358362.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="ACG8M5_001091"
     CDS             28396..29202
                     /locus_tag="ACG8M5_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358363.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="ACG8M5_001092"
     CDS             complement(29377..29946)
                     /locus_tag="ACG8M5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358364.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="ACG8M5_001093"
     CDS             complement(30029..32089)
                     /locus_tag="ACG8M5_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0358365.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="ACG8M5_001094"
     CDS             complement(32366..33844)
                     /locus_tag="ACG8M5_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358366.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT        10255 a         7082 c         5747 g        11034 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctctttta
//
LOCUS       DAZKMZ010000020        33319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000020 DAZKMZ010000000
VERSION     DAZKMZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.020"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..717)
                     /locus_tag="ACG8M5_001095"
     CDS             complement(265..717)
                     /locus_tag="ACG8M5_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0358367.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(730..1140)
                     /locus_tag="ACG8M5_001096"
     CDS             complement(730..1140)
                     /locus_tag="ACG8M5_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0358368.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1415..1627
                     /locus_tag="ACG8M5_001097"
     CDS             1415..1627
                     /locus_tag="ACG8M5_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0358369.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1630..1842
                     /locus_tag="ACG8M5_001098"
     CDS             1630..1842
                     /locus_tag="ACG8M5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358370.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1982..2137
                     /locus_tag="ACG8M5_001099"
     CDS             1982..2137
                     /locus_tag="ACG8M5_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358371.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2134..2358)
                     /locus_tag="ACG8M5_001100"
     CDS             complement(2134..2358)
                     /locus_tag="ACG8M5_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0358372.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2436..2624
                     /locus_tag="ACG8M5_001101"
     CDS             2436..2624
                     /locus_tag="ACG8M5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358373.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2621..2794
                     /locus_tag="ACG8M5_001102"
     CDS             2621..2794
                     /locus_tag="ACG8M5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358374.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2763..2891
                     /locus_tag="ACG8M5_001103"
     CDS             2763..2891
                     /locus_tag="ACG8M5_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358375.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2950..3285
                     /locus_tag="ACG8M5_001104"
     CDS             2950..3285
                     /locus_tag="ACG8M5_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358376.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3518..4459
                     /locus_tag="ACG8M5_001105"
     CDS             3518..4459
                     /locus_tag="ACG8M5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0358377.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4461..5351
                     /locus_tag="ACG8M5_001106"
     CDS             4461..5351
                     /locus_tag="ACG8M5_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0358378.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5394..6194
                     /locus_tag="ACG8M5_001107"
     CDS             5394..6194
                     /locus_tag="ACG8M5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0358379.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6209..7060
                     /locus_tag="ACG8M5_001108"
     CDS             6209..7060
                     /locus_tag="ACG8M5_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0358380.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7261..7722
                     /locus_tag="ACG8M5_001109"
     CDS             7261..7722
                     /locus_tag="ACG8M5_001109"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358381.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7748..8200
                     /locus_tag="ACG8M5_001110"
     CDS             7748..8200
                     /locus_tag="ACG8M5_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358382.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8306..8500
                     /locus_tag="ACG8M5_001111"
     CDS             8306..8500
                     /locus_tag="ACG8M5_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358383.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8531..8824
                     /locus_tag="ACG8M5_001112"
     CDS             8531..8824
                     /locus_tag="ACG8M5_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358384.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8755..9294
                     /locus_tag="ACG8M5_001113"
     CDS             8755..9294
                     /locus_tag="ACG8M5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0358385.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9369..9836
                     /locus_tag="ACG8M5_001114"
     CDS             9369..9836
                     /locus_tag="ACG8M5_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0358386.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10491..10655
                     /locus_tag="ACG8M5_001115"
     CDS             10491..10655
                     /locus_tag="ACG8M5_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358387.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            10652..10882
                     /locus_tag="ACG8M5_001116"
     CDS             10652..10882
                     /locus_tag="ACG8M5_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358388.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            11067..11612
                     /locus_tag="ACG8M5_001117"
     CDS             11067..11612
                     /locus_tag="ACG8M5_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0358389.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            11709..11930
                     /locus_tag="ACG8M5_001118"
     CDS             11709..11930
                     /locus_tag="ACG8M5_001118"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0358390.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(12087..12251)
                     /locus_tag="ACG8M5_001119"
     CDS             complement(12087..12251)
                     /locus_tag="ACG8M5_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0358391.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            12353..12874
                     /locus_tag="ACG8M5_001120"
     CDS             12353..12874
                     /locus_tag="ACG8M5_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0358392.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            12861..13301
                     /locus_tag="ACG8M5_001121"
                     /pseudo
     CDS             12861..13301
                     /locus_tag="ACG8M5_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(13341..13499)
                     /locus_tag="ACG8M5_001122"
     CDS             complement(13341..13499)
                     /locus_tag="ACG8M5_001122"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0358393.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            13592..14353
                     /locus_tag="ACG8M5_001123"
     CDS             13592..14353
                     /locus_tag="ACG8M5_001123"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0358394.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            14540..15892
                     /locus_tag="ACG8M5_001124"
                     /pseudo
     CDS             14540..15892
                     /locus_tag="ACG8M5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            15908..17128
                     /locus_tag="ACG8M5_001125"
     CDS             15908..17128
                     /locus_tag="ACG8M5_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0358395.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            17163..17840
                     /locus_tag="ACG8M5_001126"
     CDS             17163..17840
                     /locus_tag="ACG8M5_001126"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0358396.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            17837..19030
                     /locus_tag="ACG8M5_001127"
     CDS             17837..19030
                     /locus_tag="ACG8M5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0358397.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            19045..19371
                     /locus_tag="ACG8M5_001128"
     CDS             19045..19371
                     /locus_tag="ACG8M5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0358398.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            19375..19710
                     /locus_tag="ACG8M5_001129"
     CDS             19375..19710
                     /locus_tag="ACG8M5_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0358399.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            19707..20102
                     /locus_tag="ACG8M5_001130"
     CDS             19707..20102
                     /locus_tag="ACG8M5_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0358400.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            20099..20470
                     /locus_tag="ACG8M5_001131"
     CDS             20099..20470
                     /locus_tag="ACG8M5_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358401.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            20486..21046
                     /locus_tag="ACG8M5_001132"
     CDS             20486..21046
                     /locus_tag="ACG8M5_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0358402.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            21171..21482
                     /locus_tag="ACG8M5_001133"
     CDS             21171..21482
                     /locus_tag="ACG8M5_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358403.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            21506..21697
                     /locus_tag="ACG8M5_001134"
     CDS             21506..21697
                     /locus_tag="ACG8M5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358404.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            21697..25545
                     /locus_tag="ACG8M5_001135"
     CDS             21697..25545
                     /locus_tag="ACG8M5_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0358405.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            25546..26418
                     /locus_tag="ACG8M5_001136"
     CDS             25546..26418
                     /locus_tag="ACG8M5_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0358406.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            26415..27464
                     /locus_tag="ACG8M5_001137"
     CDS             26415..27464
                     /locus_tag="ACG8M5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358407.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            27468..28292
                     /locus_tag="ACG8M5_001138"
     CDS             27468..28292
                     /locus_tag="ACG8M5_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358408.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            28310..30370
                     /locus_tag="ACG8M5_001139"
     CDS             28310..30370
                     /locus_tag="ACG8M5_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0358409.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            30387..30536
                     /locus_tag="ACG8M5_001140"
     CDS             30387..30536
                     /locus_tag="ACG8M5_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358410.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            30533..30979
                     /locus_tag="ACG8M5_001141"
     CDS             30533..30979
                     /locus_tag="ACG8M5_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358411.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            30981..31118
                     /locus_tag="ACG8M5_001142"
     CDS             30981..31118
                     /locus_tag="ACG8M5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0358412.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            31153..31395
                     /locus_tag="ACG8M5_001143"
     CDS             31153..31395
                     /locus_tag="ACG8M5_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0358413.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            31411..31608
                     /locus_tag="ACG8M5_001144"
     CDS             31411..31608
                     /locus_tag="ACG8M5_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0358414.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            31619..32635
                     /locus_tag="ACG8M5_001145"
     CDS             31619..32635
                     /locus_tag="ACG8M5_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0358415.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            32750..>33319
                     /locus_tag="ACG8M5_001146"
     CDS             32750..>33319
                     /locus_tag="ACG8M5_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358416.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSE"
BASE COUNT        11650 a         5523 c         7201 g         8945 t
ORIGIN      
        1 taaatctgat agtgtttctt ccttacttaa tataaatctt tctctatcat gtaatttatt
       61 tagtttttca tcattttttt gttctaattc ataagcttct ttttttattg tgttagcttt
      121 cgacagttta gtgattccta taattaataa aataagtcca atcaataata acattcaaaa
      181 ctcctcattt ctttgatata attattgtag agtttactca gaaatgagga aagagtccat
      241 gtgaaagcat gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg
      301 aagccctaaa tattcaataa tttggaactg actcatatca gataaatctt gttcatctat
      361 tgttctagtt aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat
      421 acaactaagg ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg
      481 ggctatttca tgccataata caaatctttt catagaaggt gataggtttt gattaattaa
      541 tatcacataa catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt
      601 ttcaattaaa tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc
      661 aaactctaca aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc
      721 aaagcccacc tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt
      781 taaggcaagt gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg
      841 ttcaccataa taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg
      901 taaagcaata tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg
      961 tcctaaaaga taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc
     1021 agatggagta cttttattcc atctagacat gcttccattt ccgaaattta actttctttc
     1081 aagttctgcc aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat
     1141 aggatatgca ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt
     1201 tacaaataga gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa
     1261 gactaaaagc aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct
     1321 gtattttgta tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa
     1381 atattagaaa atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat
     1441 tgctttagta aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat
     1501 tacttattta cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat
     1561 cgaagaactt ttggatatca agttagaagt agattctaat gatcagccat cagaacagga
     1621 ggcgtaaaaa tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct
     1681 gaaagtactt atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat
     1741 aacgcagtta cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca
     1801 tggcgttcta aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat
     1861 tcaccacttc tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt
     1921 tttcattttg gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga
     1981 catgaaggaa gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat
     2041 tgaaacagaa gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga
     2101 cggttatcgt attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt
     2161 tccgtgacta tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc
     2221 ttgattttta gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc
     2281 agtcgcttta gaatttccag cacctttgac attccaacca ccattggaat taggtacaac
     2341 atgttgattt tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga
     2401 aatggagaaa ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac
     2461 atcaaattct cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc
     2521 ttagaaaagc cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg
     2581 atttgagaat gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact
     2641 ttttatagga ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg
     2701 aagcttgttc gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa
     2761 ctatggcacg agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa
     2821 atagccgccc tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa
     2881 taaaaaagtg actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa
     2941 ttatatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa
     3001 aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt
     3061 tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa
     3121 aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg
     3181 aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat
     3241 ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa
     3301 ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct
     3361 ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg
     3421 ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa
     3481 ccaccatatg aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat
     3541 gagccatcaa gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac
     3601 aattttagga ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca
     3661 agtagtactt gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt
     3721 ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt
     3781 tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa
     3841 tgccattctg gaatgcaaaa cagccaatca atttctcggt aaagaatggg aaggcgaaga
     3901 ggtaccgctt agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta
     3961 ttgttacatc gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga
     4021 tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct
     4081 tggaggtatc gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa
     4141 gtatgcagat gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga
     4201 acagaaaaac gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga
     4261 caacgagatt atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa
     4321 catcatcagt tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa
     4381 atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac
     4441 cgaaagggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa
     4501 agccacagaa acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa
     4561 caatgagaaa gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg
     4621 ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga
     4681 gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat
     4741 atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat
     4801 tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat
     4861 taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt
     4921 ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg
     4981 agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc
     5041 ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt
     5101 acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt
     5161 ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg
     5221 gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg
     5281 ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt
     5341 taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca
     5401 caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata
     5461 tgtataaatt atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt
     5521 ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca
     5581 catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga
     5641 gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca
     5701 gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca
     5761 aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg
     5821 aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact
     5881 cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca
     5941 aaaagccgaa tctgcaaagt tgggcagatg acattcggaa aatgatcgag atcgacaagc
     6001 ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat
     6061 cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa
     6121 atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg
     6181 agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct
     6241 gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg
     6301 atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg
     6361 gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct
     6421 gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt
     6481 tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt
     6541 aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg
     6601 aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg
     6661 atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa
     6721 attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat
     6781 caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc
     6841 agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct
     6901 agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag
     6961 aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc
     7021 agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa
     7081 agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct
     7141 caacaagaga gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa
     7201 agatttctca ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa
     7261 ttgccaaaaa tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat
     7321 tctgacgtaa catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg
     7381 atcaatgttg atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg
     7441 ttcgatatgg ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt
     7501 gccaaaaaat atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca
     7561 gagtgtatgc gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag
     7621 ataccacttt ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga
     7681 agcaaaacac attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg
     7741 aaaagtaatg aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc
     7801 aaaaatggaa atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag
     7861 ctatgactca gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt
     7921 tatctactat ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat
     7981 tgaccgactg tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga
     8041 taaggacctc gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag
     8101 tcttaaagtc gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt
     8161 tgaaactgca cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag
     8221 aggaggcaga aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga
     8281 tttttaactg gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag
     8341 tctcaaagag gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacga
     8401 attttttaag cagattaccg acattagcat tcagcaagga atggaagacg aagacggaaa
     8461 ctttatcgat gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg
     8521 aggaagcgga atgaaactaa aagacggatt ttactccagc ggccacggca tcggcggttt
     8581 aatgctagat atgccgacaa agaatcctaa aacacgtaag aaaccaaaat tcaaagtcgg
     8641 tgacatggtt cgctgcgaag cagaaggatt catctatcca tttcgtggat atgtagaaca
     8701 cgtctataat cactcagcaa tcattcgcat tgaaaacacg atggaatgtg ataagtggtt
     8761 agcgaaaagc aaagagaatt tagcagtggt gagattggtg gacatggaag ttataaaatc
     8821 ttaattttgc ttttttacaa ctatcctaca acaatgagcg ttattccaca actggaagga
     8881 gaagaggcaa ttgtgaacat tttagagaat atagatatta agcaaacaag gaaaaatgct
     8941 agacgattac taaaaagata cagaaattta gaacgtttgg tcggaccagt gaaaatagac
     9001 ttttccgtga tgactgttac caaaaatttg aaattcacaa ttgacagtca aaacgaagaa
     9061 atcattgaag cgataagtac tcgagattcg gttatcgaag cattaacacg gctaagtaga
     9121 atccatttcc aggtacttta ttatagttat tgttttccta ataagatgtc aatgtatcaa
     9181 ataggagaaa aattaggata ttccgataga actatcgaga gaatgaaggc agtagctttg
     9241 gttgaatttg ctgaggcgta taaatcagga gaactcattt cacgtacaaa ataaaaaaag
     9301 tcggattcct ccgactatga ataatatttc cgacataagt attatatcat aattggggga
     9361 atcagaggat ggtacttttc gatgtaaaga aatatgagac accaagcgcg aaagatgttg
     9421 atatggaacg cacaaaacat aatgtcgctg tgtttctttc agcatatcta tcagctagat
     9481 gtagagtagg gcaacatcga gaaccaaaag tgacagcatc ttattccttg gttccacctt
     9541 ctacagctga tcatatattt gaagcagaaa gaatgctgat cgataaagaa gaagcacagg
     9601 aagaatttga gtatctgcat aaattgtttg ttcgaggcta ttcagcgata cagcatccgc
     9661 ataagcctga tgtgactgaa aggcgcaaga agatattcta tgatcgttat atcaatggtc
     9721 tgcccattta tgtaactgct caaaggaata atactagcga agaatcggtt aaagtagaat
     9781 caaacagaat tatcatccaa tttgcttcat cgttagaact ggttgctttc aagtagccag
     9841 tttttacact ttttataccc ttttattacc aatttggttt ccattttata ccttttttat
     9901 accaatcact tacctattca acgttgtatt atgatagtgt cgaaaaatta ggaaacagga
     9961 tcgacaaaat aaaatgtaag ggaggaaatc tccctcatcg tttaattaag cttcgatagg
    10021 cagcaacgga aatattaaga ataaggatgt gaatttcaac tccttctaaa ttgttcttat
    10081 tatctatcat ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtga
    10141 gcgaagagaa atgattccat taataatttc aatttttgcg ctttgtctta atgtctatat
    10201 gattggattt aaaaatgggc aaaataaaag atagtagtaa ctaagaaaga aatttttata
    10261 ttgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
    10321 ctatgcaatg ttcgattcat tgccagtgac tttggcaacc gaggcatcgg cggtttaaaa
    10381 atataggggt gcgcaatttc gtacgcgttt tgtgcatcgt gtaggttgct attacatatt
    10441 agatcactcg ttgagtggtc tttttatttt acataaagga ggtaacaacg atgtatagac
    10501 cacaatactt agaacagaag tatgaagtaa tcactgtgca aaacggtaac ggtgagatag
    10561 tacgaaagta tagaagacca ataaagagcg atacatataa acgaaaggaa aacaatgaag
    10621 ttatttcttt tcgcagaagg agaaaagcca aatgagaaac tactggtatg tatcgctaac
    10681 taatgaatat cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat
    10741 caaagggaag tactctattg tcgaaatgct aagagaagct acaccaaacg aagtggataa
    10801 atgcaagctg atatattgcg gtcatggcta ttggaaagac gagtatatcc aacaaaacat
    10861 tgagaggtgg atagatagat gagttacctc gaacatttga aacgttgcta catgcattct
    10921 aagaataaac ttcctgacag ctacactgta gatgatgttg ctatacatgt attgaagact
    10981 gagagccata gcagtcctga tggtagcagt aaggagcaga cgctcgcgtg gttcaagttc
    11041 tttaagtgga ttaaggagga agagtaatgc acatgcacat aggagaaaag atatataaat
    11101 tgagacggct taataacgaa acaatgaacg acttagttaa acaaactgga gtgtcaaaat
    11161 catggttatc tgatattgag tctggaaaaa agcaacgcgt tgattttaat aagatatgtg
    11221 caatagccaa acattataaa gttagcttag aatactttcg ggatgattat ttggatagtg
    11281 atgaacacga ggatattatt cctttgacag gcgaaacaag agttaaatca gtgaaatcgc
    11341 ttgaacaaag cctaaagtat ctgaataaca aacaagaacc taaccttcta gagatcaaac
    11401 taaaagatac tgactcagta ccagaggttt ggtacaaggg tgagaggttg gatgaattgc
    11461 ctaaaggatt agtagatgtc tcgtatcatt ggaagactga tgattttact aatgatgata
    11521 gaggagcgaa cgacatcacg attcaatact tttctagctt taatgataag tatccagata
    11581 agaaaacaat cggacacaag agagatatgt aaatgaaaga agttagacct agagacgaaa
    11641 tagacaaact atacaagacc aaacgatggc gagacctaag gcaagtagta atagctaggg
    11701 acttcggcat gtgccaagag tgcaagcgtc gagggcggaa cacaaggggc acgatcatcc
    11761 atcacatagt cgaggcgagg gaagacctgt cactgttctg gtccgtagat aaccttgaat
    11821 gtatctgtgt agcttgtcac aacagagagc atccagagag atcaggcggg aagaagaaac
    11881 caaaacctaa atcacatatc gttaaaatgt attcaactcc tgaaagataa gtttgcagcg
    11941 aaatgaaggt agccccccta ctctaaaaga ttaaagagta aggcttgaga agaacggtgc
    12001 tgtccttcct tcgtaaaaag accgcttttc aagttttttg gagaaaaagg aaaaagccga
    12061 ccaatttaag ccggctttgg acgaagctat ttcttagtcc atttgtttcc tttttgagaa
    12121 gtagggggta atcggtcgcc tggatcaatt tttacttctt gtccgccttg gacatttcca
    12181 ccacgaggac ccacttcttt gtaggttcct tttggtttgt tgtcttcgcc gggtttatag
    12241 agttctccca taggaatccc tccttaacaa atttcggcac agcactgccg ataacctaat
    12301 tataaggatt gtgataacga tttcaatcta tcttttgaaa gaaggtgata ttatgccgca
    12361 accagcgaag agtgcaaaat tacaattatt aaacggaaac ccaaataaga agaataccga
    12421 agaactccgc aagcgagcgg ccgcagaaga caaattaaaa atggctactg acaaaatcaa
    12481 accgccgtta tggctagatt cgctaggaaa ggataccttt gagtttatcg ccgatgaatt
    12541 gctgtctgtg gatttaatca gtaatccgga cgtccataca atggctctat actccaattg
    12601 gtattcgcaa tacgtttctt tagaaaaaca gcttcgaaaa ctacaacgag agtacaagtt
    12661 gaactatgcg cttgcgaaaa aggaggcaga ggcgagaggt gagccgttta atgaacctaa
    12721 tgaattaatt ggtaatccgc tctctcggca gatggataca gcgtctcgga atctccgttc
    12781 ttttggcgct gatttaggac tatcaccagc agccagagct aagttagcta ttaagatggc
    12841 tgatgatggt ggtgatgacg atgacgactt ctaatatttt ggatatgtct tacacagaac
    12901 gtgtggacta ttggcaaagc tatcttgagg agcaagcttc ttggggtggc tttttaaaat
    12961 gtccatatcc ggaattgtta actacttggt atgcggaacg attaatcgat ggaagcatac
    13021 cagccagcaa agaaaatatt caagctgcta aacggcatat gcgtgatttg cagcgccaag
    13081 gaacagatga ttttccttgg atctttgacg aagaaaaagg tcaccggcct attagatata
    13141 tcgaaaaaaa atgtaaacca actgaaggcg actttggttc gtttgttttg caaccttggc
    13201 agcatttcat aattggatcc atgtacggat gggtacatcg tgatacagga gagcgtcgct
    13261 tccgcgaggc tcttattttt gttggacgta aaaacggtaa gttttgccgt ttaaaaatcg
    13321 ggcaaaatcg gtgaaaacct ttatttttgg ctttctttta gtaataattc gatagcttta
    13381 tcaagcaatt tagacatagg aaccatagtt tcttccgaca ttttttttaa tccttcgtat
    13441 aaatctttat caattgcgtt agaaattcta attctgtttt ttagtccgta tttgtccatg
    13501 tgtgacactc ctttttattt tactataaca tccgattcca ccgcttgcaa ctaccatcaa
    13561 ttgatggtag tatataaaag aggtgagtag attgaaaaaa tacattattt acaaacactt
    13621 aaataaaata aatggcaaaa tttatatcgg tgtcacaaat gatattggca gaaggtggag
    13681 gagcggtggg attgaatata aaccaccaaa gaacgaaacc caacaccaca gaagtttttg
    13741 gaatgcaata caaaaatacg ggtgggaaaa ttttgatcat ttgattattg aagaagactt
    13801 aacgatgaaa gaagcttttg agaaagaaaa gttttatatt gaactttatg actcaactaa
    13861 taagaaaaaa ggttataaca tcgcaaaagg cggaaatggc ggaataatat ataaagttca
    13921 ccccaaagga atgctaggga aaaaacaatc taaagaattt tcttcaaatc acagtaaatg
    13981 ggcaaagaac cataagaata attgtatgac aaacggtgat gttgtatggg gtgttactca
    14041 cgaacaccca aaaggcatgt tgggaaagca ccacagcaaa gaatcaatta tgaaaaagaa
    14101 agcatatagt ggtgaaaatg ctgtaacctc aaaaccaatt gtcgcaattg aacgggatgg
    14161 aactaaaaga gaatttcaca gcgctaagct ttgcatggct tactattcga ttagtacatg
    14221 tgttttttac cgacttttaa aagatggcgc cccttatgtg ataaatccta aagcgaacta
    14281 cagaaataaa gaaaaaatat tagctatcga aggaattatg ttcaaacata aagaagatac
    14341 cgaggtaagt tagtagattg cgaaaggtta ctaactaccg taacgcgtag gagttgaata
    14401 aatataatgc tcccaagagt gtccgacagc agataatttg ctgaaaatgt acgctaaact
    14461 gggccagaac tgactgaccg atgaaaatga gggtgacctc cagagtgcga gataaaaagc
    14521 tcgcagataa taacaatcga aaacatcact aatttcaggc ctttccacat acatggtcgc
    14581 ttatgatgat gaacaaggcg ccaacgttta cgtattggca aatgctcgtg atcaagcaag
    14641 cttgttgttt gataaggccg cagaaatggt caaacaatcg ccggcgctct ttaagaaatt
    14701 tggtaagcct aaacgatcaa gtattaatta tgctcccgcc ttttctaaaa tggaaccacg
    14761 cgcctcagat agccggaaat tggatgggct aaacactcac tttggtattt ttgacgagat
    14821 ccacgagttt acgaattaca atctgatcaa cgttatcaag aaatcaagag gaaccagaaa
    14881 acagcctctg atagtttata tcacaactgc tggatatgta ttagatggtc cgttgatgtc
    14941 ttattttgag caaggtgtgg actgtttgga acatttggaa gatgacatcg atgaacggac
    15001 tttctattat ctggcaaaac ttgacagtgc ggaagaggct gatgacccaa gattatggat
    15061 caaagccaat ccgaatattt gtctaatgaa ttttgttggc atgctagatg actatgttaa
    15121 ggataaaaaa gatccaaaag aatatgctga ctggattacc aagcaattta acttgttttc
    15181 cgatatcgat gagctgtcat ttgtcgatat gcctaccatt aaacgaaaca ataaaaccat
    15241 cgatattgaa acgctcaaag gtaagaagtg tgtcggtggt tttgacttgt ccgaaacgga
    15301 agactttacc gcagccgttt tagaatttcc gcttgaaaca ggcgaggtat tcattttgca
    15361 acacacatgg atcccacaag ctagatttga tcgagataac aatcaagagc gtatcaaagc
    15421 gtgggagaag gtgggagatc taacgattat tcctggtgat tacgtcaatt atgaatacgt
    15481 cttaaattgg tttgtagaaa attcgaaaat ctatgacatt gtaaaaatca attatgacaa
    15541 ggccaaggcg ctacgattaa ataaagaact agaaaatgca ggatttgaaa ccaacgagat
    15601 tcggcaaggg tttctatcat taggtgggcc aatgcaaaac ttcaaggaaa tgctattgga
    15661 cggtaaggtg attttcaaca attccaagct ttaccgatgg tatctatcca acgtcaagct
    15721 ggtgatggat cgcaactcaa actggatgcc gtctaagcag tccaagagta gaaaaataga
    15781 tggttttgca gcaagtttga acagccacgc cgaagtgttg aatatgttgg ttaatcctgt
    15841 cggaaccggg aaagtaacct attactcgat ttccgattta atgaatatgt aagaaaggtg
    15901 tggaggaatg agtattttag atcgtttgcg ttcttttggc cgagcgaagc cgaaagcgag
    15961 caaacaagag tattttttga atgacccggg attgataccg tatttagtcg gaaaagatga
    16021 aatatcagaa gggatttttt ccgtaattag ccgtgtatcg aacgtttttg cgtctctccc
    16081 tctcaaaatg atagatgtgg agtttggcca accggacgac tgtcctgcat acaacttgtt
    16141 gagcgaaggc cctcgatatt ttacaaagtt tgattttttc cgggacgtgg aagttttgag
    16201 aaactaccaa gggaatgcgt atgtgcagat tttccgaaat atcaatggag aagtagcaga
    16261 tatggcgtta gtaaaacctg gtgcttgcca tccagtgatt gatatggata gcggggagct
    16321 ttactaccaa gtaactgcga ctgacaaagg cagttacaag caagttatct atgtacatta
    16381 catggaaatg cttcacttta aacaaccgag gtttggcggc ttggaaggta cagaccccac
    16441 aaaagtatta acgaataccc tcggatatga tcgagaagtc cgaaaaatct ctttaagtca
    16501 gcttaaagga agtaatgaag ggctaaaagt taagtttgct agcaatatgg atgaagaagc
    16561 taaaaaagct acagttaaaa acattgctga tttttatcga caaaacggtg gactacttgt
    16621 ggaagaaaac ggtgtagaaa tcgaacgttt acaacgagag ctggtagaca gcaagctttt
    16681 agatactgat aaagtatctc gctccagaat cgctatggtc tacaatgtgc cggagcattt
    16741 catcgggaat aaccagtcga gttactcctc tcaggaacag ctcaatatgg aatttttgac
    16801 gtacaatcta gtaccgaccg ttaatcaata tgaagcggaa ctaaataaga aaacactatc
    16861 gagaaccgaa aaagcaaaag gttatcgata taagtttaat gtcgcaagtt tgctaagagc
    16921 tgacactcag gcaaggggac aattctatca aatcatgcga cgaggcggag catactcagc
    16981 gaatgatgtc cgaagatttg aggacttgca accgataaat aaaaccggta tggatgatta
    17041 ccatatttcc ggagacctat atccaatcga tatggatcca acattaagaa aaacaacctc
    17101 gtctaaaagc gtagccgaaa acggttaggt ttttttagtt tgcaccgaag ggaggtggaa
    17161 aaatgaaaaa agtgacgtta agcggcgatg tcgtggataa cgataccgcg tggctttatg
    17221 actggtttgg gatcaattgt atctcaccag ggaaaatttc tgccgctctt acagaagcag
    17281 cgggggatga agtagaactt gatatctcat cgaacggtgg ggatgtccta gcggcaagcg
    17341 aaatatatac cgctatccgt gcctatccgg gaaaggtatc cggaaatgtt gtgagtattg
    17401 cagcaagcgc tgcaagtgta atcgcttgcg cttgcgaacc gcttagaatc tcacctacgg
    17461 cacacatcat gattcataac gcatgggtga ccactagtgg caacgctgag gaattaaaag
    17521 ccaatgcaga aatgttaagc agtgtggatg agtctattgt taatgcttac gagatcaaaa
    17581 caggactcga tcggaaaaaa cttgctgatt taatggcgaa agatacttgg ttaaatgctc
    17641 aaacagcagt agcagaaggg tttgcagatg aaattatgtt tgcagaagca ccagtaacgg
    17701 tactcaatgc ctctcaaccg gttattccaa aaaacgcagt aactaagttg aaaaatttaa
    17761 tactcaaagc ggaagcaccg caagaagaaa cactcttaca gaaaaaacta aaagccttaa
    17821 atggagggaa aaacgaatga atttagaaca attaaaaaat gcgtgggtcg aggcgggaag
    17881 taaagtctct gacttaaatg cacaactcaa tgcagcattg gttgacgatg aaaaaacaga
    17941 agaagatgta gtaagtttgc aagcacaagt aaaagcagca cgggctaaac gggacggatt
    18001 gaaagagcaa gtggcaaata tggaagccga acaagtctta aacgtcaaaa aagaaccatt
    18061 agataaaaaa gatgaaaact tgaaaaacaa gtttatcaaa gactttaaag cgatggtcaa
    18121 tggtgatcct gctattatgg ctactttgac atctaatacg gatgaatctg gtaatgctat
    18181 cggattgact attcctgtag atgtgcaaac gactattcat actttggttc gtcggtttga
    18241 ctctttgcaa gaatacgtaa acgttgaaaa agtgaccact accagcggtt ctcgcgttta
    18301 tgaaaaatgg tctgatatta caccgttgac tgctttggat actgaagacg gtgaaatccc
    18361 agcaaatgat gatcctgcac tttacttgat caaatacttg atcaaacgct atgcaggtat
    18421 ttccacagta actaacagct tgctaaaaga tactgccgaa aacattttgg catggttgtc
    18481 taaatggatc gcgaaaaaag tagttgttac tcgcaataca aaaatcttgg cagctattga
    18541 tggaatcaaa gcggcacaaa agaaagatgt tacagatgtt gatggaatta aagatatcgt
    18601 aaacgtccaa cttgatccag ctatcgaagc tacatctatg tttattacaa accaagatgg
    18661 cttcaatgtt ttagataaag tgaaacgtgc tgatggatct tacttgttac aaaaagacgt
    18721 agcttctgca actggatata ccttcttagg taaaccaatc aagaaaattg cttctcgttt
    18781 cttgccaaac aaaggtacgc aagctactcc taaatatcca ctgtacattg gtgatctgaa
    18841 agaagccgtt acattgtatg atcgcgaaaa catgagcttg ctgacaacga atattggtgg
    18901 tggagctttt gaaacagaca ccactaaagt acgcgtcatc gatcgcttcg atgtgcaact
    18961 agttgatgat gaagcggttg ttttggctac ttttacaact attgcgaacg agacaccggc
    19021 gaaagtttaa ggagctgatt ccttatgatt cttgatccta aaatggattt aggcgaaatc
    19081 aaaaacgcac taaagattga taccgatgat gacgatgtgg aagtaagccg tgcggcacaa
    19141 gctgcaattg catacattag aggggctatc gggaatgata agccctcttt ttataagcaa
    19201 gaaaatgaaa cggttgatct gattaattta gctatcctgc aattagcaga tcattattac
    19261 aacgctcgat cagcaaccgt gagtgggaac ttgcgagagt acgatttagg ttttacaagc
    19321 ctaatcttgc aactcaaagc aagttatttg ctttttgtgg aggaggagta gcgtatgccc
    19381 cttatccaaa caggaaattt aaatcaacgc atcaagtttg tccgagatac gacggttaag
    19441 gatgaggacg ggcaagttgt cccgacttct acaaccattc ttacttgctg ggcaagtgtg
    19501 cagacacaac gcctgaacga tattaagacg tcgattggta cggctttgga aggaacactg
    19561 acgttcatta tccgctacca acaaaaatca gagctgacca atgatatgaa agtgcgttgg
    19621 aatggaaaaa cgtttgaaat cattacgatt acgaaaggcg agtttgcgaa ggacttcacg
    19681 acaatcattg caaaagaggt ttcaaaatga gtgtagaagt cgatgcaacc gaagtgtaca
    19741 aagcgcttag ggaagtaaaa gcaaacgttc aacgagtgga aagcccagca cttagaaaag
    19801 ctggggagta cgctcaagaa gagttacgac aaaacacacc ttactgggat ggaacgaagt
    19861 caaacggtaa acgtggttcg tatatgcaag aacatgctaa gaaccatgtg gttacaagct
    19921 cgataaaaaa cggattggta gaagtcggct atgacaaaga tgtttcttgg cggatgcact
    19981 ttatcgagtt cggaacaatc aaacaacgtc caaaaggttt cgtacaaaaa acacaaaagc
    20041 aaatcgaaaa acaagtaaca caaatcattg ctgacgaagt aaaaaggagg ctaggacttt
    20101 gaaaacggca gtatcacaag tctattcaat tctgaatagc aatgaaaaaa caaagaacat
    20161 tgatttttac accaatagtg ttccggaatc agctcaaaca gtacctagcc ttccagttgg
    20221 cagaattaca gagatatccg gcaactatga agattttgca agcaacaatc ctttgaccat
    20281 tcaatttaac gtacaggtag atgtatgggt gtcaaccatg aaagaggttg atgcctttta
    20341 ttttgccctt gatgaggtta tgagggggaa tggttggcaa tgcgcataca cggaacaaac
    20401 agatgacgag gacttggaag gtgcaaagcg gattatcaaa cgatatgtag caaatatttc
    20461 actaaattaa aaggagagaa aatagatggc aacagtagga ttcgagagcg tcatttttgg
    20521 cgtaaaaaca ggtgcaggcg gcactctaaa agaattagta gcagataagt cgaaaggcgg
    20581 agcgatcgaa gctaaaatta ctggattagg cgcaacttct aacacaacat acgcttcaaa
    20641 cgtaccgttc ttcattgcaa gtaaaggggt ttcgtcgcca aaagttacgc ttgacgtggc
    20701 agacttaatg gataacggca tttacagcga aatcattggt gctaaaaccg tggatggtgc
    20761 aaatgtaatt ggttcagaaa ctgaagcgcc ttacgtgtcg gtagtcatgg ttacagcgaa
    20821 caaagaagga aaacgcttgt tcatgggatt gacaaaagga aaattcagtc atccagatat
    20881 cgacatgaaa acagctgaag acaaaggggt agaattgcaa accgattcca tcgaagggga
    20941 attcatttct gatgaacgtg gctatgtata cttaacagcc gtagaatcag aaggaatgac
    21001 cttacaaaaa ttcaaggact tggtaaataa caaagagggg gagtagttaa ccctgcatct
    21061 acaccaacga cagatacagg gacaccaaaa gaaccagaac caaaaattga tacacaaggt
    21121 tagccatttt tggctagcct tattttttgt aaaaacaagg aggaaaacaa atgattgaat
    21181 tgcaattgaa acttgacgga aagaaaaaaa cattcaaaca acaagatatt tccgcacgtg
    21241 caatgcgtga gtgtatcaaa ttttacgaga aagcggaaaa agcagaccta actgatttag
    21301 aagcgattga ttcaatgatt gcaattacag cagatatttt ccaagatcca gcagttacat
    21361 ttgatgctat tttagacggt ttgactgcga gcgagttagt accggcatta gaaagtgttt
    21421 ttgaacaaat aaatgaactg ggaaacaatg aaaaaaagca gacggcgagc aaaaagaaat
    21481 aagtttttct gaagctagga aagcaatgga tcaaatctac aaagatttaa tcgaagcagg
    21541 ttggacgatg agagatgtgg acgaagccga ctatcattat ttgttacacc tttttggaga
    21601 agtggagagt ggcgaagaat atgtagatgg tgctgatttc atcaaacaat ttttatcggc
    21661 tgaagactta gtaaaacttg aggaaggagg taaataatgg caggaaaagg acaaccggca
    21721 ggaaatatca agctagggat tagtttagat agcactagtt ttggtaacac gctggacgaa
    21781 atcaatgcga aagtcaaaca agctgagtcg aatatgcgtg ccaatctaaa ggcttatgat
    21841 tcagcaggac gttcatacga agcacttagt caaaagacga aagacttgtc tacggttatg
    21901 gaagggcaaa acgccaaagt aagagaatta acaaagcgcc gtgatgaagc gattagcaag
    21961 tatggcgagg aatcgaaaca agttgctaac cttaacacac agataaacaa tgctaccgca
    22021 aaatataatg cttacagtcg ccagttgaac gacacaaaaa aagaattggt gtattccaaa
    22081 acagccgtca atgatttatc taatgaaatc aaagaaaatg aacgacaaat gaacgccgaa
    22141 gtaaaagcgt tgaaagccgc tggtgatgaa tctggtgctt ttgaagcaaa acaaaaaggg
    22201 ctagccaaac aaacggaatt atccgagaaa gctatcgaag aacagcgcaa agttgtgaaa
    22261 ctgatggctg atgagtttgg cgattcagca aatgaaaccg aagatgcaaa aagggcatta
    22321 gaaaagttag aacgacaaag ccaaatatct agcaggcaat tagaagcact caaaagctcc
    22381 agcgatcaat caggaaaaga aatagaagat tttggcgaca agtccacaag gtcagctagg
    22441 aaactggacg gactaaaaga caaattaagc tcgctaaaaa gcgcattttc gtttggtgca
    22501 gttgctggat tagcgcataa cgctattagc agtgtagtaa gtggcgtgca aggtttggtt
    22561 ggcgaagcag taaacgcatc ggattcattg atgaagtttt ccaaaaccat ggagtttgct
    22621 aactttggga agtcacagat agaaagctcg aaaaaagaaa tgaaagacta cgccgataag
    22681 acggtttatg gtttagaaga aattctgaac acaaccgcac aattggcatc taatgggatt
    22741 cctaactata cagaactaac caaggcggca ggtaacttga atgccgttgc aggcggttct
    22801 agtgatacat tcaaatccgt tgccatgatg ctaacgcaga cggcaggagc tgggaaacta
    22861 acaactgaaa actggaatca attagcagat gcgataccgg gtgcttcagg attgttacaa
    22921 gacgctatgt tgaaaaacgg agcttataca ggaaacttcc gtgatgcaat ggcgcaaggt
    22981 caaatcactt ccgacgagtt caaccaagca attgtacagt taggtatgaa tgacggagca
    23041 gttaaggcag ccacttctac agacacattg agcggttctt gggagcagat gaaatccact
    23101 gtaataaatg ggctacaaag tattatagaa aaaataggcg ttgaaaatat cactggtttc
    23161 atcaacagag taacaaaagg gattgaaaat tctattccta aaattactca atttataggt
    23221 tggttgagag atattggaac gtggatcgtt gaaaatagag agccactaac atggattgtc
    23281 ggaatcatag gcggaattac attagcagta aaagcattga acgtagcaag tatgttgctg
    23341 gcaattactg gcggaacatt ggcagcccct tttgtggcga ttggtgtagc attaggcgca
    23401 ctagcaggtg ctttggtgat agcttataca aaatctgaaa catttagaaa tatagtcaat
    23461 gcggctttta cagctgtgaa aaacgtagtt atgagcgtta tcaataattt ggtggaatac
    23521 tacaaaatgt tgtggagcgt gttgcagtgg ctttgggaaa aaataaaaga atgggcttca
    23581 tggattggca ataaattcat tgaaatgaag aacagcgttg tgaatacggt tcagaaccta
    23641 tggaatggcg tgaaaaactt cttcagcaat ggcgttggag atacgtggaa taaggtagtc
    23701 ggttgggtaa aaaacatttt caacaaagca actgaattga agaacaacgt ttctgatgta
    23761 atcggtaacc tgtggaatgg tatcaaagac acattccgta gaggtatcga tacggtattc
    23821 aattggtttt cagaactacc aacgaagatg aagaatgcca ttattggcgg taaaaacgcc
    23881 attgttgatg cgttcaaaag tattttcaac gcagcactta aagcgatagg taaaccagtt
    23941 aacgcaatca tccatggagc ttcatgggta ctagaaaaat tgggcgcaga acctctacaa
    24001 gaatgggatg taccacaata cgctacaggt acaccagcag gtgggcaccc aatcaatggt
    24061 ccaatgatgg ttaatgatgg acgtggagca gaaacagtta tcacaccaga tggtagagcc
    24121 ttcattccta aaggacgcaa tgttgttcta aacgcaccaa aaggaacgca tgtcttgaca
    24181 gcagaagaga ccgcgcaact tcaaggttct aaagctccta agtatcgtta caaaaaaggt
    24241 actaacttct ttggtaacat gtgggatagt gtgaaaaagg ttgctggtaa tgtaggtcac
    24301 acaattaaaa acgtagtagg tgacgtgtgg gactttattt cagacccggg agcgttagct
    24361 agaaaagtac ttggggggtt agatgtatta ggtggattga caaagtatcc attagaagta
    24421 ggtaaaggca tcctatctaa agcaacaagt gcactgactg aaaaaatcac tgggttgttc
    24481 tcatctggta acttagatac ctccgtagga acaaatggcg tctataaata tttggctgat
    24541 gttgctaagt ctgtgatgaa gaaattccca ggatttgtgg caactagtgg gtatagacca
    24601 ggtgaccctt attcacatgg taaacgtaat gccattgata ttgcattacc aggcgtcaca
    24661 ggaggctcac ctagatacac agaagcagca aactacgctt ttgacaaatt cgcttccaaa
    24721 attggttacg taatcactaa tgggaaagtt cgtgaccgtt caggacaatc aggtcaacca
    24781 gcaactggtg catgggagcc atggcccgat ggagatcact atgatcatgt gcatttaaac
    24841 ggtgtgaaag acccacaaaa cactcaaatt tcaggagata gcgtgggagg cagtggggta
    24901 gaaagatggc gcaatgtagc aattagagcg ttgaaaatga ccggtcaata cagtactgca
    24961 aacttaaatg cattactaaa tcaaatgcgt acagagtcaa atggtaatcc taatgcagtt
    25021 aacaattggg atattaacgc caaaaatgga acaccatcaa aagggttgct ccaagtgatt
    25081 gacccaacat tcagacagta tgcaatgcca ggattcaaca gcaatatttt tgacccacta
    25141 tctaacatct tagcttcaat cagatacgca ctatcaagat atggctcact aacaaatgcc
    25201 tatcgtggag ttggttacgc aaacggtgga attgtaaacc aacatcaaat tgcggaaatc
    25261 gcagaaggaa acaagccaga aattattatt ccgttagata aggctaaacg atcaagagcg
    25321 atgcaattat tgtctatcgc aatggacaaa ctaggagttt ctcctaaaac ttatggaaat
    25381 gcgacaactg tttccagtga ttacgaaatc ctagaagcca ttgaaaaaca ggcggcaaca
    25441 acgaatcagc tgttatcgtt attgctggca tttttcaaag gaaatagccg aactgataga
    25501 gatttagctt tagatattca gaagatcttg gttaggagga tgtagatgcg aactgtatta
    25561 ctgagaaata gaataaacga agaaattgat ttgtccacag aagactattt tgctactggg
    25621 ttgagtaata tggggtttga agtaaaaaaa gaacatgtgg gacagtgggg gaatttcaga
    25681 gaaagcagtg agagcgttga aatatctgaa tttcagtcat ctgtaatcat ttctgtgcat
    25741 gggtttcgag aaaaagaact gtacaattca cttgtgcagt tcctatcgga aggtccgttt
    25801 gaactggaat ttgcttttga cggcgaaaca atggtaagaa gatgcagtct aaaatcttta
    25861 agtaagactg aaatcgatcc gaaaacatcg ttactaacag acactttaga actatatttc
    25921 acatcaaact ggtattccgt aaaacgcgaa aaattaattc agcgaccgaa tgtagtgaaa
    25981 acacgtggta aggtttttcc gtataaaaga tcttatatct acactcaaaa cttgtgggag
    26041 aaaaaaggtg tattcaaatt caataataat tctgtatacc taacgaatag caaggaacgg
    26101 atgtccccgc taaaaattcg tgtgattggg aagtgttcaa atccgtattg ggaagtaatc
    26161 caaaactcac aaatcatcgc aacggatgga tacttcatag atatgactga aacacagact
    26221 ctagaagtat caagcctttt tgaagataca acggcgattt taaaagatat tgcaggggta
    26281 gaatcttctg tctaccaaca acaggattat acaaaaacta attttgttca ggccccaact
    26341 ggagaattca gcattgtgtt tcatgttggg ggggcagacg tagagattga gttatacgag
    26401 gagcgtgatc tgttttgatt ttagcagtca cgctttttca tcgtgattta atgctttata
    26461 acgaacattt attctctaac agttttgaat ttggtgtgga cgagatcaac gaagaggcta
    26521 gtagctttac gatggataag tacgttcctg taaaaacagg cgattttcta ctagcaaaat
    26581 atattcctag tggaaaattt gcatattttg gtgtaattac gtcgcaggaa gacgaaaaaa
    26641 tcagttgtaa aagcttactt agtttagctg atagtgagat accgactgca cgtgtgtcag
    26701 gagataacta cgaagagcat atccggcggt tgattgaata ctatttgctg aatgatccaa
    26761 cgaaacaact aaaagatatt ttagacgtca aagcagaaag tgcgacctct cattcgtatc
    26821 aagctaccga tacgaataaa cacaagttaa gtgcgtatat tctcaatggt ttcaaaaaat
    26881 acaacgtaaa atggtatttc aagggaattc aaaacagaaa aatttataca ggcatacgtg
    26941 ctgtaaatga atcaatttac attaaagaca attcttctga atttagcgat tgggatgtgt
    27001 ttgttcaggc gccgggcgct ggaaacgaga acaagctatt aatcgttgat aaagcaatga
    27061 aagatataga gaaaccgata atactgtcaa catggtattt ggacgaagaa aacaatttga
    27121 cacaagatgg atcgaaagaa aatatcacga aaccaactgt caatttggtt aatatctacg
    27181 atcaaaccgc agaagataaa gcatcctacg aagatgtggc aaaatcagag ttgaaaggca
    27241 atacgtattc gcatgaaatc aaagtgaacg ttgtaagaaa tgcaaaaaat ttgaatgtcg
    27301 aaacgattga aacagggatg tttgccacga tttcttataa aggaaagata tacaagtcgg
    27361 ttttgacagc ttggcgaata tcaagtgata aggagtttgt ggaattgact ttcggaaaca
    27421 taagaagtcg ttttatggat tattttgaag ataatggggg ataaaaaatg gttagcaatg
    27481 tggatggata tcaatttgaa aacgtgaaag taagcgcaga aaatgatgct agactttatc
    27541 acgttttata taatcggaaa aatcaggtta ttgatggtta cgatcagtct atgaatttat
    27601 cttcaagcgg attaacagta aaagttgctg caggagcagc gattattcaa ggtcgtatgg
    27661 tcgttgttcg acaagaagaa agtataacag ttccagcaaa ctcaagtggt tatatagcat
    27721 taacagtgga tttgacacaa gaagttatac ctggatctat tcttccagaa tcggaagaat
    27781 atgaatggac taataatcaa gtcaagctag aatttataac aaaagttata aaaggtaatt
    27841 taaacaatgg tgataaggtc tataatttac cactatgctc agttaattct actggatcaa
    27901 ctgtttcaat ctcaaagata tcggatagtt acgagctgac tctctctaaa ggagaaattt
    27961 tgtggagggg gactgcgtta atgcatgata ctcaaactgt ccaaccttca aaaaaaattt
    28021 ggcagacagt ttcaggtttt ttgttagtat ggctcccata cgaaaacggg caagcaattg
    28081 aggatagata tgtaactacg cctttttata aggagcgtgt aacttttact aatgttttag
    28141 gagaaattgt ttcaggattt gatgactatc acaaaaagtg gtttagtaaa cgaataaact
    28201 ataattcgaa tactaatata tttacaggtg cagcaagcaa tgcaagtgga gataacgcaa
    28261 atatggtgct taggtatata gtttcttttt agttaggagg tatggaagag tggcaaattt
    28321 agaaattaaa ttatctgcaa ataagagaca gccttatcta cgtcaccgtg ttgttggtag
    28381 agttggtgat ggggggctca caacaatcaa tgtacaactt cttgaggaag atgaaattac
    28441 accttttgta attaatctaa acggtacttt gaaatttgtg ggcgaagttt caaacggtaa
    28501 ctataccgag ggagaaccag aaataatcga ttcgactaat gggttaatta gttacacgtt
    28561 cactaagtca aatttcagca cgagtcatca attcaaacaa gcatattttg aatatgtaga
    28621 tcctaacggc aaaaaagtaa cttttcagaa cttcatcata gacgtgttag aacgagtgga
    28681 tattaattcg gagcaagcga aatactatat ttcttcattg gaaaaattac agagtgaaat
    28741 gcaaaccact ttcaatcagt tcattagtga taaacaggtc caatacgatc aaatctactc
    28801 gaaatataac gaattagtaa gattgataaa tgaatcagat aagcaagtaa atgatcgtat
    28861 tgatcaaacc aatcagcaaa tcggcgatct cggcaagctg aaaaagatgt acagtaacag
    28921 catcgacttt gggggctatg attattcggg gaatccgaat ttgttaagta agctatcata
    28981 cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaagggtg aaaactcgtt
    29041 taaatataat aagatatcag ctgaagtgga aggtggagta gagttatatt ataaacgaag
    29101 aggtatagct aactggttac cctctaataa aacgcttgta atgactgtta agcttagagc
    29161 tggagcggac tatagtccag ttgacggaaa acttatactg attagatata ggtatgttga
    29221 cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga taactcagga
    29281 atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag cataccatcc
    29341 ttggatacaa tttagggctc aagatggggt acttggggaa atagaaatga gctatgacat
    29401 caaaatcgaa gaaggctcta cagctacacc atatcagcct aacttacttg atgactctta
    29461 ctggctaggt aaaacaccgt tgggtgagaa tttgataacg aatgccaagt tcccaattat
    29521 aacacgaagc aatcctattt ctggttttga tatttcagaa gaattaataa taggtgaaac
    29581 ttatacggta tctttaaaag ggactaagcc agctactaaa gaattccact tatactacgg
    29641 agaagcaaat taccagcagt tgcagtatca ggcaacttta cttcctgtgg aaggattaac
    29701 agatgtgtgg agtgccactt ttacagccag aaatactagt gaaacgacaa atttacttag
    29761 agtagcgtta tggcaaaaac ctaattctgc aacatatgat actgttcaaa ttgactggct
    29821 caagattgaa aaaggcgaca cacgcacacc aaatatcagt cagtttaaat actttggtga
    29881 aggcttgaaa gacagtaaca atccgaacga ctacagctgg gatgtcacac cagagtatac
    29941 tgaaaaaagc ttgaataata cggttagtct gactgaaccg caatccatcg aaggattgaa
    30001 aaatttcgaa gatggtattc aatcaaaagg aaaatctgta ttgacatcag acgacaacaa
    30061 atatgaagtc gtaaccttaa cagttacaaa cgggaatacc ggatcagcaa agctttatcg
    30121 tgaaggaaaa accgtcacta tttatttttt tgcgttgaat gggaaaagca gtggtggaaa
    30181 tgattcaacg atactaacaa ttccagaagg ctatcggcca ccaattagtt ttgagcaact
    30241 ggttggctcg atagaccgtt ctactttgaa cagtgctcag ttatctattg gtgcagatgg
    30301 agccattaaa tggcgaagaa actcaagtta tggatcggat tatacctttg caattactta
    30361 cacgatttag aaaggcgtga atcgatatga aggcagcata tagaccaatt gaaccttacg
    30421 gattcgagca aatcattgtg aatgatgaag aacatttacc ggaagaatgc acagaagtcg
    30481 aaccaccgat tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa
    30541 catttggaaa tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct
    30601 tgtatccgtt ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc
    30661 gctaacgatt gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa
    30721 cgcgttagat cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga
    30781 aaacggcaac ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca
    30841 ggaaaatact gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat
    30901 gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc
    30961 ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg
    31021 gctgttttac tgacgatcaa gttcgtctat tcgttccact atgtatcaca gatgaagaag
    31081 cagatagaat tattagcaaa gaagagagcg catcttaagt gatgcgtttt tttgttggaa
    31141 agttggtgga acatgaaaga agaagcgctc caagacgttg tggagagatt agtaagaatt
    31201 gaaacaaaat tagacaacta cgaatcactt agagaaaaag ctgatagtgc aaaagatttg
    31261 gcagacaaag cctactcagt agcgctaaac aacgcagaag atatcaaaga aatgaaagcc
    31321 aataataaat ggtcgtgggg ttacatgatc ggtttaggca ttacgatcat tggctatttc
    31381 ttgactaaat tgtaaaggag ttaagaagaa atgattttac ccgataagta ttatcaagtc
    31441 attaaatgga cggttttaac agtattacca gctgcatctg ttttagtagc cacgttagga
    31501 aaagcgtatg gatggaatgg aacagatatg acagtactca ctatcaatgc agtagcaaca
    31561 tttttaggcg ttatcactgg tgtgtctgca tataacttaa aaaaatagga ggaaaaaaat
    31621 gaagaagaaa atcattttat cattgagcct aataatggct ctttttttat tgccaattaa
    31681 cgggttcgcc tatacgatca acaatgaatt taatttaggt gtaaatgaag gtagctcaca
    31741 agtagcaaat aaccaataca tcctattgca tgaaacagca aacgaaactg cgacaggacg
    31801 aaatgaagcg cagtacatga aacgttcttg gtacaatgcc tatacagcgt atattgttgg
    31861 cgatggtggg atcgtttacc aagttggaca acctggttat gtacaatatg gcgctggttc
    31921 atatgctaat gcaaatagtc ctgtgcaaat cgaactgcaa catacgcacg ataaagtaac
    31981 gtttgaaaag aactacaagg catatgttga attagcgaga gattccgcta ataaatacgg
    32041 tattccactt acattagaca caccgtataa tcaaccagga atcaaatcac atttatgggt
    32101 aactcaaaac atttggggcg atcacactga tccgtatgga tacttatcag aaatgggtgt
    32161 aagtaaggaa aaactggctt acgatttagc tcatggattt acagacgaaa acccaacgac
    32221 ttctgagaac aagcctgtca ttgatccaac ccgagcaggt gcagcaaatc caactttatc
    32281 agatggaacg aaccattctc acattgatca gtttggagaa atcgaaaatg cgaacttaca
    32341 cgtcgctgga tggcacatcg ctaactataa atacgagtat attttcatta tggactacaa
    32401 tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagaccgg atgtaaatca
    32461 agcttataat acttctggaa acgttggcta tcatgtatct tttaacatgc gcaatttccc
    32521 taataagaaa gtatacgtca tgatgcgggc aacgaatgat ccagaaggaa acactaaagg
    32581 cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata
    32641 gctcctcgtt gaggagcagt acatagaatt gaaaattaac gttaaatcaa aaaaatattt
    32701 acaagatgtt tatttaagta gataataaaa gaaaaaagca ggtgaaattg tgacagcaga
    32761 aattggtatt atgaataaaa gtggaatcgt gttagcttca gattcagcat ctacgattgg
    32821 agatagcaaa gtatataata ctgcaaaaaa attatttact ttggattcca tgcattctgt
    32881 aggtatcatg atatatggta atgctgaatt taatggtatt ccttgggaaa taatcattac
    32941 tcaatataag aaaagcattg gtagttctgt ttttaatact ttggaagaat acgcggataa
    33001 ttttatagaa tttgtcaaaa cagcatcttt tattagaagt gaacagaccg agcaagaaca
    33061 aatgattggg gtttttcaaa aaataatttc aggtttattt gagagtatag aagtagatat
    33121 taacttttta ataagccaag gtacacaaat agacaaagat gtcctagtga agttattaca
    33181 aacaaaaatg aataccaatt tgtctcaaca gtcccagaca ttcattttag atattgaaaa
    33241 ggcgttattt cttagtaact atggagaaat attaaaagat attcttaata gtatttctac
    33301 aatggaaggt gtttcagaa
//
LOCUS       DAZKMZ010000021        32326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000021 DAZKMZ010000000
VERSION     DAZKMZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.021"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..805
                     /gene="truA"
                     /locus_tag="ACG8M5_001147"
     CDS             59..805
                     /gene="truA"
                     /locus_tag="ACG8M5_001147"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0358417.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(1260..3929)
                     /locus_tag="ACG8M5_001148"
     CDS             complement(1260..3929)
                     /locus_tag="ACG8M5_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0358418.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(4167..4355)
                     /gene="rpmB"
                     /locus_tag="ACG8M5_001149"
     CDS             complement(4167..4355)
                     /gene="rpmB"
                     /locus_tag="ACG8M5_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0358419.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            4625..4987
                     /locus_tag="ACG8M5_001150"
     CDS             4625..4987
                     /locus_tag="ACG8M5_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0358420.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5039..6721
                     /locus_tag="ACG8M5_001151"
     CDS             5039..6721
                     /locus_tag="ACG8M5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0358421.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            6838..8874
                     /gene="recG"
                     /locus_tag="ACG8M5_001152"
     CDS             6838..8874
                     /gene="recG"
                     /locus_tag="ACG8M5_001152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0358422.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            8923..9924
                     /gene="plsX"
                     /locus_tag="ACG8M5_001153"
     CDS             8923..9924
                     /gene="plsX"
                     /locus_tag="ACG8M5_001153"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0358423.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            10046..10288
                     /gene="acpP"
                     /locus_tag="ACG8M5_001154"
     CDS             10046..10288
                     /gene="acpP"
                     /locus_tag="ACG8M5_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0358424.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            10556..11560
                     /locus_tag="ACG8M5_001155"
     CDS             10556..11560
                     /locus_tag="ACG8M5_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358425.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            11561..12505
                     /locus_tag="ACG8M5_001156"
     CDS             11561..12505
                     /locus_tag="ACG8M5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358426.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            12505..13467
                     /gene="opp4B"
                     /locus_tag="ACG8M5_001157"
     CDS             12505..13467
                     /gene="opp4B"
                     /locus_tag="ACG8M5_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0358427.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            13494..14408
                     /locus_tag="ACG8M5_001158"
     CDS             13494..14408
                     /locus_tag="ACG8M5_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358428.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            14434..16215
                     /locus_tag="ACG8M5_001159"
     CDS             14434..16215
                     /locus_tag="ACG8M5_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0358429.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            16563..17249
                     /gene="rnc"
                     /locus_tag="ACG8M5_001160"
     CDS             16563..17249
                     /gene="rnc"
                     /locus_tag="ACG8M5_001160"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0358430.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            17269..20850
                     /gene="smc"
                     /locus_tag="ACG8M5_001161"
     CDS             17269..20850
                     /gene="smc"
                     /locus_tag="ACG8M5_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0358431.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            20859..21668
                     /locus_tag="ACG8M5_001162"
     CDS             20859..21668
                     /locus_tag="ACG8M5_001162"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0358432.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            21679..22677
                     /gene="ftsY"
                     /locus_tag="ACG8M5_001163"
     CDS             21679..22677
                     /gene="ftsY"
                     /locus_tag="ACG8M5_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0358433.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            22864..25131
                     /gene="gshAB"
                     /locus_tag="ACG8M5_001164"
     CDS             22864..25131
                     /gene="gshAB"
                     /locus_tag="ACG8M5_001164"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0358434.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(25220..25672)
                     /locus_tag="ACG8M5_001165"
     CDS             complement(25220..25672)
                     /locus_tag="ACG8M5_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0358435.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            25822..26772
                     /locus_tag="ACG8M5_001166"
     CDS             25822..26772
                     /locus_tag="ACG8M5_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0358436.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            26765..27724
                     /locus_tag="ACG8M5_001167"
     CDS             26765..27724
                     /locus_tag="ACG8M5_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0358437.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            27721..28476
                     /locus_tag="ACG8M5_001168"
     CDS             27721..28476
                     /locus_tag="ACG8M5_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358438.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            28499..29452
                     /locus_tag="ACG8M5_001169"
     CDS             28499..29452
                     /locus_tag="ACG8M5_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0358439.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(30096..30392)
                     /locus_tag="ACG8M5_001170"
     CDS             complement(30096..30392)
                     /locus_tag="ACG8M5_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0358440.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            30747..31103
                     /locus_tag="ACG8M5_001171"
     CDS             30747..31103
                     /locus_tag="ACG8M5_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0358441.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            31113..32012
                     /locus_tag="ACG8M5_001172"
     CDS             31113..32012
                     /locus_tag="ACG8M5_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0358442.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        10710 a         5732 c         6906 g         8978 t
ORIGIN      
        1 aattttatag aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat
       61 ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca
      121 gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg
      181 aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca
      241 agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga
      301 tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca
      361 cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg
      421 cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat
      481 tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg
      541 aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac
      601 aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat
      661 cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga
      721 tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta
      781 tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata
      841 agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag
      901 aagctacttc tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt tgacctgagt
      961 attagagaaa caaacgggaa aatagctcct catattttgc cgtttgttga gctaataccg
     1021 aaggtcagct gattgaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga
     1081 gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg
     1141 acgaggcgtt gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg
     1201 atggaacgtt gttttgattg cccttggtgc ggacagtctg aacaggttcc atcgtttctt
     1261 tattttctac gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc
     1321 gtgccataaa aggggcgatt gcggtcaagg taccagacat aaagaataaa caatgcaata
     1381 cataggacaa aaaggattgc acctatcgta aatccttcag gcaagtaact gaaagtgaca
     1441 gtatgtttac ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg
     1501 actggtttcc catcaatttt agctttccat cctttgtcat aaggaatagt tgtcaccaac
     1561 atggaatctt cagcggtatt tacagtccca gaagcttttc tgccagtggc ttccatttcg
     1621 acaccttttt ctttgatctg attcattgcg gcttgatagg ctacaacatc tagaccaacg
     1681 actttaggct ccatgaaact tatttctttc gtcccataaa aacttgcagt aaatgtcact
     1741 gttgtctctt gaggataata gccaagatca tagtattgac cagaaatgtt gatttgcgtt
     1801 tttcgcgatg taccattgac agtgatcgtc gcactagaac tttctaattg cgcaaaattg
     1861 gttgggaaca aacttaagta agcttgtgta tttgcaggca catggacttc ccaagtgagc
     1921 tcttttggct ggttatctgc tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc
     1981 gtcgtattca actgattctt caaagtaggt gtatagaatg taaaatactg ttcatttaga
     2041 ccagacaatg tattgatcaa gtttgtctga ctggtcaaat tatctgtcac tggctgctca
     2101 acatcattaa cggacaacgg tgcagtaaat gctagtggca atgcattgac attctgatac
     2161 agtgtatagt cgccgctttg accagcttgg cggaagccat atttactgaa cgtgctttcg
     2221 tttttagaaa tgttatattt gattcctgta aacccatcca tcagcaaagt attattgtta
     2281 taacggatat tcaaatttgt ccctcttgaa cggaaaccta atttatccag gtaagaggaa
     2341 gaattacgat ttcttataga agaaaacaaa ctgataccac tgtaaccata gttgatcgaa
     2401 tcgtttgcag atactggatc cagattttcc aagcggtaaa acgagtcgga atcttttttt
     2461 gtttcattta caagagcttt caagtcagga taaggatcag tatataaact gcgggaagca
     2521 taattccaat catcaagaat gccatggatc atactgttcg tattgaccag tgcttcagca
     2581 gacatgatca ataataacaa tatactcaga tgattgaatg aaagtttttt atgctgataa
     2641 aatcccacag caaaagcata aatccctaag aaaaccactg tcaatacaaa ggaagtcatc
     2701 gtcacatagg tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa gagagcagct
     2761 acgaccaaaa tgattcctaa caacagccca cgatcgtctt gttctatctt ttcccatcct
     2821 tttcctgcaa gcaggagcac taagaaagag aacagatagg cataacggaa taaaaacata
     2881 ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa caagataaaa acttgcgata
     2941 agtatggcga aaagactgcc gtacaaaaat ttatttttca acggtacttt ccgactggca
     3001 aaataaaaca gacagaggac taatggtagc agacctgcgt aaacgaacgg aatcgatccg
     3061 tattttgtcg tgtcataaac accaatcata tttttcatga ttagatccag ataagaagtc
     3121 gcttctgttt tcaacgtagt gatctgactc agtgcctcgc catttgaccg aagatccagc
     3181 actgctggca atacaatgat cattgaagcg ccgcctgcca gtaaagaagt gataccataa
     3241 ggaacaatcg tttttttata ttctgaccag tttgtcaata gacgggcgat aaaatataag
     3301 aaagaaaata cgccaatcat aaaacccata taaaaactgg ataaaaacaa gagtaaataa
     3361 ctgataaata aaagtttcgg ccgtttaaga tccataacac gatgaatccc caaaatcact
     3421 aatggaagat aaaaaaatgc atccagccac atgatgatct ccgaatgtgc agtgataaat
     3481 gagatcaatg cgtaactgac gctaagtgca acatgccccc agcgaggaat cttgaacgta
     3541 cgtgcaagaa accaaaaagc caatccggca cagccgacct taagcaaagt cagaacatac
     3601 agtgcatctg gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct
     3661 agatagtaag aaattaaaga taaataattc aatcctagag aagcattcca tgtataaaaa
     3721 atactttgct ttccaagaag aacatttcgg aagctggcat gaaaattaga aaactgtgaa
     3781 aaggcatcac ttgccaaaat acttctgctg cttccaggat agattccgat acttagataa
     3841 acaagtgcta aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg tccgttttct
     3901 ctaaagaatg actttaaatt ttttttcata tttattttcc accaaatttc tatgaagttg
     3961 tattcagttt atcatatcct tgtttttctc tgcttaaaaa gagtctagtt ttcacaaaaa
     4021 aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata aacgcaaaaa
     4081 aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa accgttcact
     4141 atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga tttcaaagca
     4201 cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac tttttgtaag
     4261 ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt tccacttttt
     4321 gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt tgccttgatt
     4381 tggagcaata gaataattca gctcatacca gactaacata tcatatagaa tgggaatttg
     4441 caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa ttaaacaagg
     4501 ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt tatttcaagt
     4561 tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt aaggaggctc
     4621 attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca atgatgtaat
     4681 tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa
     4741 aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact caagaggagt
     4801 agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta tcagctatgg
     4861 tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc
     4921 attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc gtgtactgcc
     4981 agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga ttatctaggt
     5041 gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg
     5101 tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc cagatggcga
     5161 tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag tagattcaac
     5221 ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg
     5281 cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc aaattccaga
     5341 tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag ttgaaactgc
     5401 gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta gagaagcagc
     5461 gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt
     5521 tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag tcctaaaaga
     5581 agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg gctttttaag
     5641 tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag cagaaatgga
     5701 tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga cagaagatat
     5761 ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta cagtcgatag
     5821 tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt ctttacttgt
     5881 ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag gagaagtctt
     5941 gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca
     6001 gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc
     6061 acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga
     6121 aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa gtacggaaga
     6181 cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc caaacaataa
     6241 gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag tagcagttgt
     6301 tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg atcaagcttc
     6361 tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga gcggtcaagt
     6421 cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag gagacttttt
     6481 gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa cagcgttaag
     6541 tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg caggtgatga
     6601 aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac atcctgattt
     6661 ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt ctgctgaata
     6721 atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc ttcggttagt
     6781 gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga aaaatttatg
     6841 cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag agcagctagt
     6901 cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc ttttcgctac
     6961 gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt tactttgaaa
     7021 ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa
     7081 tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca accttatcta
     7141 aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt
     7201 aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt ttcgccgata
     7261 tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa aaaagggttt
     7321 caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga gaaatatcga
     7381 ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca cgaagaaaac
     7441 catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa
     7501 ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta tgatgtccaa
     7561 aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca aaaacgtgta
     7621 acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact tttgcaggga
     7681 gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac cgttacagca
     7741 ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc
     7801 ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg gtcaacgaaa
     7861 gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc
     7921 ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct agttattacg
     7981 gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga
     8041 ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat tacagcatat
     8101 ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc gatcgaaaca
     8161 cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa agaactggca
     8221 agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc attagatgta
     8281 aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt
     8341 ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga agccttcaaa
     8401 gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt aaacgtgccg
     8461 aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca gctgcaccaa
     8521 cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt tgctaatcct
     8581 aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa tggttttgta
     8641 ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt ccgtcaatct
     8701 ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc
     8761 agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc tgagtatcag
     8821 cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga ttagcagact
     8881 gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat
     8941 gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt agcgcagcaa
     9001 gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa gaaatactta
     9061 acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag tgatgatgag
     9121 ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc acaagcagtg
     9181 aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct actagcagca
     9241 gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg
     9301 ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc taacgcagaa
     9361 aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc acgtaaagtc
     9421 cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt
     9481 agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt gaattttatt
     9541 ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt cacagatgga
     9601 tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat gatgaatcta
     9661 ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa
     9721 gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg tgccgttttg
     9781 tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga tgctgttcgc
     9841 tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca attagtcgaa
     9901 caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt ccattgaaaa
     9961 aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt aattgacaag
    10021 tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa
    10081 aattatttca aatcattttg agatagatac cgataaagtg acggatgaat tgaacatcaa
    10141 agatgattta aaagctgatt caattagtat tatggaattc gttttagagc ttgaagatga
    10201 attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt
    10261 agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct
    10321 gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt catttttctg
    10381 ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct atataattat
    10441 taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt attctgtgta
    10501 atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta ttcgattgtc
    10561 cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca aagatgacta
    10621 ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt tagcaatcgt
    10681 tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat tacttgatcc
    10741 taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa tcgacctgaa
    10801 cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc aagatccgtt
    10861 gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac tttcctatca
    10921 cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag aacaagtagg
    10981 gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag gcggtatgag
    11041 acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga
    11101 accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac tgaaagacct
    11161 tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag tcgtagcaga
    11221 aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag caggtgtaaa
    11281 agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact ctataccaca
    11341 agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa
    11401 catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag caagcgatca
    11461 tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt gctcctgcta
    11521 taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat taatcacaat
    11581 caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag taacggatca
    11641 tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat atggtttagt
    11701 gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat tagaaaaggt
    11761 aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa
    11821 aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca gtttgaatcc
    11881 taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac ggttaagcga
    11941 tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc cggaagatgc
    12001 gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag gagtagcccg
    12061 tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg ctctggatct
    12121 atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat acggcctcag
    12181 ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca atatcgcaat
    12241 tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata atgacccgcg
    12301 acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag aaaataggga
    12361 aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc aaaaagaata
    12421 ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac acaaagtggc
    12481 gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg
    12541 atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc gatgccgggg
    12601 gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga acagatgcga
    12661 caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat cggaaatgct
    12721 ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga cttgatcggc
    12781 gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac ttatttgatt
    12841 gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga taagattgtc
    12901 gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt gatcatgcta
    12961 tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac actaggtttg
    13021 caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt accatcaatt
    13081 acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga agtaatcgat
    13141 tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac gaataaagta
    13201 ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa
    13261 atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta tccgggagtc
    13321 ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac atccttagta
    13381 ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat catgagtatc
    13441 gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga tcgatgagtg
    13501 aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga atgattgctc
    13561 gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg gttttattat
    13621 tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat gttagtatct
    13681 tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc attctgggag
    13741 cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga aactctgtta
    13801 cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta gggatcatct
    13861 ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat tttatcatga
    13921 ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga tacaatgtct
    13981 ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga ttattccgaa
    14041 gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca atgggaacca
    14101 atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta atcattacta
    14161 atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact ttcttaggtt
    14221 tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca agtaacggag
    14281 aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt ttagttttga
    14341 tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt
    14401 taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt tggatttgca
    14461 gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga tacctctgga
    14521 aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat tgcaacaaaa
    14581 aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac ggacagtcag
    14641 tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc ttttatggct
    14701 ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga cgacggagct
    14761 gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc
    14821 aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta cgaaatcatt
    14881 gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat cgtaggtatg
    14941 gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat agacgacaaa
    15001 acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg tggtggtatc
    15061 tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga tatggaatca
    15121 agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag taatatcgtt
    15181 gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac gcctaaattg
    15241 aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa
    15301 ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa agaaattgct
    15361 ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt caagttaggt
    15421 aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat ggctgataaa
    15481 tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga acgtttctat
    15541 gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc ataccatgat
    15601 gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt agatgatgct
    15661 ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa taaactaaca
    15721 atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga ttactatatc
    15781 caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct gatcgaattc
    15841 aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta ccaagctgct
    15901 tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc tgcgttcaac
    15961 tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag ttcaagcaaa
    16021 gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa
    16081 gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc
    16141 aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc tgttacagca
    16201 gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt
    16261 tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc atgacttctc
    16321 caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg caaaaatatg
    16381 gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt tttcacaact
    16441 tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac catttcaaat
    16501 ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa ggaatgtgga
    16561 aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt tttcacgatc
    16621 taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat cgcaatctgc
    16681 aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa ctgatggttt
    16741 ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca aaaacacgag
    16801 ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac tttgatcaat
    16861 atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca gcattgcttt
    16921 gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc gacacgaccc
    16981 atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt tcacatgaga
    17041 tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat
    17101 accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc gaagtttatg
    17161 tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca gaacaagctg
    17221 cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa
    17281 cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga ttttgaaaat
    17341 agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac agaggctatc
    17401 cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat gccggatatc
    17461 atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac agtgatttta
    17521 gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac ccgccgttat
    17581 cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt aaaagatatc
    17641 caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat ttctcaagga
    17701 aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca
    17761 gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct ttttgaaaca
    17821 gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca gttaactcct
    17881 ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac actgactcaa
    17941 accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa
    18001 caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc gatccaagaa
    18061 caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa
    18121 aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga aggacaaaaa
    18181 gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata tcaaacttca
    18241 ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa
    18301 gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat aaaaacacag
    18361 caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt aagagatcaa
    18421 tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta tctagaacgt
    18481 caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa
    18541 gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca ggcgcagcta
    18601 aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag
    18661 ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa tcaagtccag
    18721 cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt tggtttttac
    18781 caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt cggagcagtg
    18841 gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc tttaggtggg
    18901 gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg
    18961 aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa accaagacaa
    19021 ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg gattgccagt
    19081 gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct aggaacgatc
    19141 cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat
    19201 cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac aggtggagcg
    19261 aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa acagttgact
    19321 agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt tcaggagctg
    19381 caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg aggcgaacaa
    19441 ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa tgaacttgaa
    19501 cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca gcagtttatc
    19561 gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct tgaatcgcaa
    19621 cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc
    19681 agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa
    19741 gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa agcagaggca
    19801 accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga cttttctgat
    19861 catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc tcagcgagaa
    19921 acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca aaaggagatc
    19981 gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa
    20041 caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa tcatttgacc
    20101 tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta tcaagaaaca
    20161 accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat tgaaaaactg
    20221 ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag acatacattc
    20281 ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga aacgatggat
    20341 gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat ccgtcaagaa
    20401 ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt actgacggaa
    20461 ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg caaaaaactg
    20521 caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc gttgctcttt
    20581 tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga agcagcgtta
    20641 gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga tgacacacaa
    20701 tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact gtacggagtg
    20761 acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa
    20821 gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg
    20881 atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat aaacaagcat
    20941 tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc cccttagctg
    21001 cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac agcatcactt
    21061 tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag aaagtgatgg
    21121 aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg ccactagatg
    21181 ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca
    21241 atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt acagatgaat
    21301 ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct aaaatcaaag
    21361 aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt atgcttttgg
    21421 aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca aaagatcttg
    21481 gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta ccgatgatcc
    21541 aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag
    21601 atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat
    21661 tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt tactggcgaa
    21721 gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga aaagacgcgt
    21781 aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt cgatgaagac
    21841 ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga aacagcgata
    21901 cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa
    21961 gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg aatcaatgaa
    22021 gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt cggtgttaat
    22081 ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa
    22141 aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca gttagttgtc
    22201 tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga tccagcagct
    22261 gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt actagttgat
    22321 actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa aataaaacga
    22381 gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt agatgcaacc
    22441 actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt
    22501 cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc tatccgtaat
    22561 gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga cttggagccg
    22621 tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga agaataaccc
    22681 atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt
    22741 aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg ttctcaaaca
    22801 atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat tggaggtcac
    22861 ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt tatcgaccaa
    22921 gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa
    22981 accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca aacagatttt
    23041 agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga attatttcag
    23101 tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga gatgatctgg
    23161 ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat tgcaaaatta
    23221 aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa
    23281 cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct aagaacattg
    23341 ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact ttatttgaaa
    23401 acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg tgcttcacca
    23461 atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt tcgcagtatt
    23521 cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta tgcttctatg
    23581 aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa
    23641 gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt agcaacaaca
    23701 ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag attgggtatc
    23761 aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg gacagaagaa
    23821 aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa agtagcacta
    23881 gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct tgaaggaatg
    23941 aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga ggaagcattg
    24001 actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga agcacagcta
    24061 tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga aagccatgaa
    24121 cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat atttatgttc
    24181 gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca atttttgcgt
    24241 ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag taaggacagc
    24301 tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct
    24361 gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt aaaagcttat
    24421 ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta cgggttaggt
    24481 atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt agccattgct
    24541 tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga atatcgtttc
    24601 tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa tgttattggt
    24661 gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga
    24721 acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca attgatgttg
    24781 aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt ttatctaagg
    24841 gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga attttctgaa
    24901 gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat tagtggtatc
    24961 gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc atatgggatc
    25021 atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc aggaaaaggc
    25081 agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta aaagaaaaag
    25141 cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa
    25201 atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc ttttttggtt
    25261 tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata tcaatcacag
    25321 gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga tttgcagtga
    25381 cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg atatattggt
    25441 tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga tgttttttga
    25501 gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca gtcatactaa
    25561 gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg ttcactaaag
    25621 gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc ataaaaaata
    25681 ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa tacctaaaac
    25741 atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg aaaatcattc
    25801 tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt agttatttta
    25861 agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact gctccagtta
    25921 gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat ttgtttgatc
    25981 cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac ccaaaataaa
    26041 tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat tttagtggct
    26101 atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt ttgctttgct
    26161 tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa aaatcaattg
    26221 atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc tacttttttt
    26281 gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa ttttgcgact
    26341 gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct cttggtttgg
    26401 ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt aaatctggga
    26461 atagattacc agaagatgca gtttttaggc attggcttag tatcattggc tagtgcagtc
    26521 atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa tcttgtctct
    26581 agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc aggtagtttg
    26641 ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga aataccagtc
    26701 agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat aaggggacga
    26761 cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg ttttatttgt
    26821 tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat gtgcattgag
    26881 gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg ccacaatcag
    26941 ctttcagaca attacgcata accagttttt gactccttca gtattgggac ttgagtcgtt
    27001 atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga tgcttcagca
    27061 gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga gtctattgtt
    27121 atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga tgtcagggat
    27181 gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca tggatccgaa
    27241 tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc agagtcagta
    27301 tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc gatatcgaca
    27361 tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga tagacacaga
    27421 tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat caacggctct
    27481 tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc gtgtcttttc
    27541 tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc tgtttctgac
    27601 aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga gtgtcgtcgt
    27661 tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga agagtatcct
    27721 atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt taaagatgta
    27781 cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg tgctggaaag
    27841 tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga aatctattta
    27901 gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt atcgatccta
    27961 cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga atttggacga
    28021 tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca ggaatcactg
    28081 gaacacattg ggttagaaga aatagcggat caatcgattg acactctttc aggcggtcaa
    28141 ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat tttactggat
    28201 gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat
    28261 ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa ctttgctgca
    28321 agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca aggaacgacc
    28381 caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat acggatttgt
    28441 gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga gagaagagat
    28501 gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag catgtggaaa
    28561 tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg aaactacatt
    28621 gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag aaaaagtcgt
    28681 cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag ataaggtagt
    28741 tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag tagaatcagc
    28801 aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag atctaatcat
    28861 catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc cgaccatttt
    28921 cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca ctgctttagg
    28981 ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat ctagtgcaat
    29041 cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca cacttgtgaa
    29101 tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc atgatttatt
    29161 tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa gtgtttctta
    29221 tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa caaaagctat
    29281 tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac aaacgaatgc
    29341 tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa gcgggggcgg
    29401 tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat agcataaata
    29461 acagaggatt aaactctcat actatgtaac aataatgttt cttacattct taaatagcaa
    29521 gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga tgattttacg
    29581 gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc tacatcatac
    29641 gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt cagcatcaag
    29701 atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa cttaaattta
    29761 gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct gcttctcggc
    29821 aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt tcttattact
    29881 caaaacaaat cgcagttgtc acaatctctt ctaaacgatg ttccatctgc tgcttctcgg
    29941 caataagccc actagactag aaaatatgag aagctatttt ctgtccagcg ttcttattac
    30001 tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat gttcaatcag ctgaccttcg
    30061 gtattagctc aaagcactga tttcacaacc tctatttagg catgtgtctc agcaagcggt
    30121 tgatagttga tgatctgcat ttttcgataa aaatcggcgg gaagcttact ccaccgtttt
    30181 agtaattgat agaaataact ctcttctgct ttgattttat actcgcttaa tatcaatcca
    30241 aaactattcg ttttttttga agctaatttt ttataaatat ccaatgcttt ggttttatct
    30301 tcattgacaa agagtgtttt tacttcaata taagaaccct cgatattttg tgtttcttcg
    30361 ataaaataga tgttttttat atattccttc atagtgattc ctccaaagaa gtcttttatt
    30421 tgataagttt actctaccga agagttgtta ataatccgtg aacaaaaagt aattcttttt
    30481 ccaatagttg ttaaaggttt tttatttttt atagtatttt ttgttattta agaaaggaat
    30541 aaggttaaac aaataacagg ttgctttttg ggtctttttc cgttcaagaa actgaacgaa
    30601 gaaaagggaa agttgtctct tttgtgataa gagaaatcat aataaacgaa tgacttactt
    30661 tccaataaat tgagaacagt cagaaccaga caaattagtt gcatctgatg aataaagtga
    30721 ttattctatg acatgaggtg atacagatga aattagaagt gacaaaagaa gcacaagaag
    30781 tattgaagaa taaattagaa gcagacgatc aacttttatt agatatcgaa aacggtgacg
    30841 gtccatttgc agaaagccaa gtcagttgcc aattggatac ggcatttcga ttgatcattg
    30901 tcaaaaaaga tacacaaaca gatctaagtc tttattctgt agtggctgat acaccagttg
    30961 gaccaatcaa aatcaaagac agtgcaatac tttacatgga tgatccatcc acacttgctt
    31021 tagaaccaac ttataatagt ttacaattaa aaggaccaag cggcttgaga aaaggcaact
    31081 tgcaagttgt tcgaaaagac tagagggata aaatgggaaa attggcgatc atcagcgacc
    31141 tacatgtgga catcaataaa ttcggcgaat cagagctgat gcagctttgg caagttttgc
    31201 aaaagcacaa gatcactcgg cttcatctag ccggagatac ggctaataaa gtagaccgtt
    31261 gcctagctgt tgtcgaattt ttcgtcaata aaggattatc tacgacattt catttcggca
    31321 atcacgagtt ggcagatgtg cccaatgaac agatgatgga ccattttcca gacccacatt
    31381 ttttaaatga acggtatatt gctttaaatg agcagaccgt tcttttaggg atgaatggct
    31441 ggtacgacta tcagttttcc gaattgaaag attacgaggc aatcgtacga atgaagcgtt
    31501 tgtattggta tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa
    31561 tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt gaggtgattt
    31621 tatccactca tttcgttcct aaacgggcat ttatcatgta tcaatctgct ccttatgagc
    31681 gttggaacaa attaaatgca tttttaggat cagaatcatt cggagaatta cttgaccgtt
    31741 attcaaatat caaacaagta gtttttggcc atacgcatcg tcgctttgaa gataaatcca
    31801 tcggtcacac gatttacagc tgcagacctt ttggttatta ttatgaatgg cagctgaccc
    31861 gagattttgt cttgaaagaa cagttgctag acagctatga tcctatgaaa ttacgtgtcc
    31921 tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac acaacatttg caagaggaat
    31981 ttgaacaagc gatgacaatc gttccgtatt gagacagcat tagtaatgtg taaaagaaaa
    32041 taccttgtaa aacataagga taagtaagag cagtagatcc atttatcgtg gatatgctgc
    32101 tttttttgcg gaaaataatc agcgaatgtt tatttgatcg gatgatttgt aactcgcaga
    32161 gtactagatg caagcgtgta ttcttttttt gaacgataat cacaaaaaaa aatataatat
    32221 acatttttta tttaattata attaatattg attgaagtct aggcagtcaa gtcgagagtg
    32281 taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DAZKMZ010000022        32098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000022 DAZKMZ010000000
VERSION     DAZKMZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.022"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1555
                     /locus_tag="ACG8M5_001173"
     CDS             128..1555
                     /locus_tag="ACG8M5_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358443.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1647..2012)
                     /locus_tag="ACG8M5_001174"
     CDS             complement(1647..2012)
                     /locus_tag="ACG8M5_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0358444.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2009..2374)
                     /locus_tag="ACG8M5_001175"
     CDS             complement(2009..2374)
                     /locus_tag="ACG8M5_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0358445.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2566..2814)
                     /locus_tag="ACG8M5_001176"
     CDS             complement(2566..2814)
                     /locus_tag="ACG8M5_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358446.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2813..2941
                     /locus_tag="ACG8M5_001177"
     CDS             2813..2941
                     /locus_tag="ACG8M5_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0358447.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2998..4692
                     /locus_tag="ACG8M5_001178"
     CDS             2998..4692
                     /locus_tag="ACG8M5_001178"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0358448.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4759..4965
                     /locus_tag="ACG8M5_001179"
     CDS             4759..4965
                     /locus_tag="ACG8M5_001179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358449.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5343..6476
                     /locus_tag="ACG8M5_001180"
     CDS             5343..6476
                     /locus_tag="ACG8M5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0358450.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6507..6827
                     /locus_tag="ACG8M5_001181"
     CDS             6507..6827
                     /locus_tag="ACG8M5_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0358451.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6856..7320)
                     /locus_tag="ACG8M5_001182"
     CDS             complement(6856..7320)
                     /locus_tag="ACG8M5_001182"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358452.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7452..8705)
                     /locus_tag="ACG8M5_001183"
     CDS             complement(7452..8705)
                     /locus_tag="ACG8M5_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0358453.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8734..9051)
                     /locus_tag="ACG8M5_001184"
     CDS             complement(8734..9051)
                     /locus_tag="ACG8M5_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0358454.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9077..9397)
                     /locus_tag="ACG8M5_001185"
     CDS             complement(9077..9397)
                     /locus_tag="ACG8M5_001185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0358455.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9861..11276)
                     /locus_tag="ACG8M5_001186"
     CDS             complement(9861..11276)
                     /locus_tag="ACG8M5_001186"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0358456.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11293..12609)
                     /locus_tag="ACG8M5_001187"
     CDS             complement(11293..12609)
                     /locus_tag="ACG8M5_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0358457.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12759..13457)
                     /locus_tag="ACG8M5_001188"
     CDS             complement(12759..13457)
                     /locus_tag="ACG8M5_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0358458.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13746..14735)
                     /locus_tag="ACG8M5_001189"
     CDS             complement(13746..14735)
                     /locus_tag="ACG8M5_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0358459.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="ACG8M5_001190"
     CDS             complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="ACG8M5_001190"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0358460.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15823..17457)
                     /locus_tag="ACG8M5_001191"
     CDS             complement(15823..17457)
                     /locus_tag="ACG8M5_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0358461.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17444..19132)
                     /locus_tag="ACG8M5_001192"
     CDS             complement(17444..19132)
                     /locus_tag="ACG8M5_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358462.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19278..20915
                     /locus_tag="ACG8M5_001193"
     CDS             19278..20915
                     /locus_tag="ACG8M5_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358463.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21060..21800)
                     /gene="truA"
                     /locus_tag="ACG8M5_001194"
     CDS             complement(21060..21800)
                     /gene="truA"
                     /locus_tag="ACG8M5_001194"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0358464.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21991..22626)
                     /locus_tag="ACG8M5_001195"
     CDS             complement(21991..22626)
                     /locus_tag="ACG8M5_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0358465.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(22773..23993)
                     /gene="sstT"
                     /locus_tag="ACG8M5_001196"
     CDS             complement(22773..23993)
                     /gene="sstT"
                     /locus_tag="ACG8M5_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0358466.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            24212..25210
                     /locus_tag="ACG8M5_001197"
     CDS             24212..25210
                     /locus_tag="ACG8M5_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358467.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25406..27241
                     /locus_tag="ACG8M5_001198"
     CDS             25406..27241
                     /locus_tag="ACG8M5_001198"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0358468.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27301..28017)
                     /locus_tag="ACG8M5_001199"
     CDS             complement(27301..28017)
                     /locus_tag="ACG8M5_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0358469.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28244..28786)
                     /locus_tag="ACG8M5_001200"
     CDS             complement(28244..28786)
                     /locus_tag="ACG8M5_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0358470.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28791..29018)
                     /locus_tag="ACG8M5_001201"
     CDS             complement(28791..29018)
                     /locus_tag="ACG8M5_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0358471.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(29097..30977)
                     /locus_tag="ACG8M5_001202"
     CDS             complement(29097..30977)
                     /locus_tag="ACG8M5_001202"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0358472.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31166..31780)
                     /locus_tag="ACG8M5_001203"
     CDS             complement(31166..31780)
                     /locus_tag="ACG8M5_001203"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358473.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31922..>32098
                     /locus_tag="ACG8M5_001204"
     CDS             31922..>32098
                     /locus_tag="ACG8M5_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0358474.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT        10124 a         6271 c         5235 g        10468 t
ORIGIN      
        1 ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat
       61 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
      121 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      181 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      241 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      301 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      361 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      421 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      481 agcttattca ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga
      541 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      601 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      661 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      721 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      781 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      841 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      901 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      961 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
     1021 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1081 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1141 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1201 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1261 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1321 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1381 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1441 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1501 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1561 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1621 tcttgataca cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct
     1681 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1741 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1801 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg
     1861 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1921 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1981 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     2041 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2101 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2161 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2221 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2281 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2341 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2401 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2461 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2521 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2581 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2641 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2701 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2761 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2821 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2881 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2941 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     3001 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3061 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3121 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3181 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3241 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3301 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3361 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3421 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3481 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3541 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3601 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3661 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3721 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3781 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3841 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3901 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3961 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     4021 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4081 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4141 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4201 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4261 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4321 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4381 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4441 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4501 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4561 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4621 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4681 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4741 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4801 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4861 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4921 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4981 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     5041 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5101 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5161 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5221 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5281 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5341 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5401 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5461 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5521 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5581 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5641 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5701 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5761 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5821 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5881 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5941 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     6001 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6061 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6121 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6181 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6241 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6301 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6361 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6421 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6481 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6541 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6601 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6661 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6721 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6781 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6841 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6901 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6961 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     7021 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7081 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7141 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7201 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7261 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7321 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7381 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7441 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7501 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7561 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7621 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7681 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7741 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7801 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7861 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7921 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7981 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     8041 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8101 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8161 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8221 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8281 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8341 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8401 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8461 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8521 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8581 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8641 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8701 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatc
     8761 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8821 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8881 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8941 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     9001 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9061 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9121 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9181 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9241 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9301 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9361 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9421 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9481 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9541 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9601 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9661 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9721 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9781 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9841 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9901 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9961 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
    10021 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10081 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10141 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10201 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10261 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10321 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10381 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10441 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10501 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10561 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10621 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10681 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10741 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10801 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10861 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10921 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10981 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    11041 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11101 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11161 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11221 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11281 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11341 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11401 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11461 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11521 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11581 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11641 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11701 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11761 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11821 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11881 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11941 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    12001 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12061 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12121 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12181 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12241 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12301 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12361 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12421 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12481 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12541 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12601 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12661 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12721 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12781 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12841 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12901 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12961 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    13021 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13081 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13141 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13201 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13261 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13321 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13381 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13441 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13501 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13561 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13621 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13681 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13741 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13801 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13861 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13921 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13981 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    14041 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14101 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14161 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14221 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14281 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14341 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14401 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14461 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14521 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14581 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14641 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14701 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14761 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14821 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14881 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14941 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    15001 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15061 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15121 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15181 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15241 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15301 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15361 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15421 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15481 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15541 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15601 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15661 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15721 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15781 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15841 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15901 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15961 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    16021 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16081 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16141 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    16201 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
    16261 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
    16321 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
    16381 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
    16441 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
    16501 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
    16561 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
    16621 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
    16681 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
    16741 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
    16801 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
    16861 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
    16921 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
    16981 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
    17041 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt
    17101 tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa
    17161 taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat ataacattac
    17221 agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa
    17281 tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg
    17341 aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata
    17401 tatactacta actataatta atccactcaa tattactact tttttattta ttttcattct
    17461 tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc
    17521 gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga
    17581 gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca
    17641 cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct
    17701 gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg
    17761 tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt
    17821 ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt
    17881 ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat
    17941 gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga
    18001 gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt
    18061 tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc
    18121 aaggaaactg gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag
    18181 ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt
    18241 ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg
    18301 ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct
    18361 gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat
    18421 accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca
    18481 caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag
    18541 caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt agacttatac
    18601 tgacagataa agcgatgact tttttttctc tattgaatat caatccataa gcataatata
    18661 aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat
    18721 caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct
    18781 ttttaaaaaa cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc
    18841 aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag
    18901 gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg
    18961 tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag
    19021 ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag
    19081 tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc atagatttgt
    19141 tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt
    19201 gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt
    19261 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
    19321 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
    19381 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
    19441 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
    19501 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
    19561 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
    19621 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
    19681 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
    19741 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
    19801 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
    19861 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
    19921 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
    19981 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
    20041 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
    20101 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
    20161 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
    20221 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
    20281 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
    20341 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
    20401 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
    20461 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
    20521 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
    20581 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
    20641 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
    20701 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
    20761 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
    20821 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
    20881 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
    20941 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
    21001 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
    21061 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
    21121 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
    21181 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
    21241 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
    21301 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
    21361 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
    21421 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
    21481 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
    21541 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
    21601 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
    21661 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
    21721 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
    21781 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
    21841 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
    21901 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
    21961 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca
    22021 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
    22081 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
    22141 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
    22201 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
    22261 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
    22321 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
    22381 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
    22441 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
    22501 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
    22561 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
    22621 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
    22681 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
    22741 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
    22801 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
    22861 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
    22921 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
    22981 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
    23041 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
    23101 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
    23161 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
    23221 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
    23281 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
    23341 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
    23401 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
    23461 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
    23521 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
    23581 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
    23641 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
    23701 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
    23761 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
    23821 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
    23881 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
    23941 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
    24001 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
    24061 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
    24121 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
    24181 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
    24241 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
    24301 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
    24361 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
    24421 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
    24481 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
    24541 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
    24601 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
    24661 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
    24721 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
    24781 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
    24841 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
    24901 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
    24961 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
    25021 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
    25081 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
    25141 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
    25201 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
    25261 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
    25321 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
    25381 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
    25441 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
    25501 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
    25561 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
    25621 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
    25681 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
    25741 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
    25801 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
    25861 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
    25921 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
    25981 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
    26041 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
    26101 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
    26161 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
    26221 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
    26281 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
    26341 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
    26401 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
    26461 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
    26521 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
    26581 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
    26641 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
    26701 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
    26761 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
    26821 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
    26881 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
    26941 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
    27001 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
    27061 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
    27121 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
    27181 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
    27241 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
    27301 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    27361 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    27421 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    27481 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    27541 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
    27601 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
    27661 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
    27721 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
    27781 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
    27841 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
    27901 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
    27961 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
    28021 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
    28081 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
    28141 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
    28201 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
    28261 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
    28321 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
    28381 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
    28441 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
    28501 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
    28561 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
    28621 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
    28681 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
    28741 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
    28801 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
    28861 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
    28921 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
    28981 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
    29041 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
    29101 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
    29161 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
    29221 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    29281 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    29341 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    29401 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    29461 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    29521 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    29581 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    29641 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    29701 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    29761 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    29821 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    29881 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    29941 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    30001 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    30061 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    30121 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    30181 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    30241 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    30301 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    30361 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    30421 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    30481 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    30541 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    30601 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    30661 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    30721 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    30781 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    30841 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    30901 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    30961 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    31021 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    31081 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    31141 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    31201 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    31261 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    31321 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    31381 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    31441 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    31501 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    31561 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    31621 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    31681 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    31741 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    31801 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    31861 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    31921 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    31981 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    32041 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgat
//
LOCUS       DAZKMZ010000023        31706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000023 DAZKMZ010000000
VERSION     DAZKMZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.023"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1018..1359)
                     /locus_tag="ACG8M5_001205"
     CDS             complement(1018..1359)
                     /locus_tag="ACG8M5_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0358475.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="ACG8M5_001206"
     CDS             complement(1359..1589)
                     /locus_tag="ACG8M5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0358476.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1876..2214
                     /locus_tag="ACG8M5_001207"
     CDS             1876..2214
                     /locus_tag="ACG8M5_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358477.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2419..2981)
                     /locus_tag="ACG8M5_001208"
                     /pseudo
     CDS             complement(2419..2981)
                     /locus_tag="ACG8M5_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3713..4294)
                     /locus_tag="ACG8M5_001209"
     CDS             complement(3713..4294)
                     /locus_tag="ACG8M5_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0358478.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4477..5103)
                     /locus_tag="ACG8M5_001210"
     CDS             complement(4477..5103)
                     /locus_tag="ACG8M5_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0358479.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5125..6456)
                     /locus_tag="ACG8M5_001211"
     CDS             complement(5125..6456)
                     /locus_tag="ACG8M5_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0358480.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6611..7012)
                     /locus_tag="ACG8M5_001212"
     CDS             complement(6611..7012)
                     /locus_tag="ACG8M5_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0358481.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7098..7304)
                     /locus_tag="ACG8M5_001213"
     CDS             complement(7098..7304)
                     /locus_tag="ACG8M5_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358482.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7399..8901)
                     /locus_tag="ACG8M5_001214"
     CDS             complement(7399..8901)
                     /locus_tag="ACG8M5_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0358483.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8919..9374)
                     /gene="argR"
                     /locus_tag="ACG8M5_001215"
     CDS             complement(8919..9374)
                     /gene="argR"
                     /locus_tag="ACG8M5_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0358484.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9472..10089)
                     /locus_tag="ACG8M5_001216"
     CDS             complement(9472..10089)
                     /locus_tag="ACG8M5_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0358485.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10310..11683
                     /locus_tag="ACG8M5_001217"
     CDS             10310..11683
                     /locus_tag="ACG8M5_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0358486.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11758..12870
                     /locus_tag="ACG8M5_001218"
     CDS             11758..12870
                     /locus_tag="ACG8M5_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0358487.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12942..14579)
                     /locus_tag="ACG8M5_001219"
     CDS             complement(12942..14579)
                     /locus_tag="ACG8M5_001219"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0358488.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14747..15355)
                     /locus_tag="ACG8M5_001220"
     CDS             complement(14747..15355)
                     /locus_tag="ACG8M5_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0358489.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15739..16890)
                     /gene="queG"
                     /locus_tag="ACG8M5_001221"
     CDS             complement(15739..16890)
                     /gene="queG"
                     /locus_tag="ACG8M5_001221"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0358490.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16909..17661)
                     /locus_tag="ACG8M5_001222"
     CDS             complement(16909..17661)
                     /locus_tag="ACG8M5_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358491.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17802..18752)
                     /locus_tag="ACG8M5_001223"
     CDS             complement(17802..18752)
                     /locus_tag="ACG8M5_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358492.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18753..19592)
                     /locus_tag="ACG8M5_001224"
     CDS             complement(18753..19592)
                     /locus_tag="ACG8M5_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0358493.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19589..20335)
                     /locus_tag="ACG8M5_001225"
     CDS             complement(19589..20335)
                     /locus_tag="ACG8M5_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358494.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20573..21943
                     /locus_tag="ACG8M5_001226"
     CDS             20573..21943
                     /locus_tag="ACG8M5_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0358495.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21998..22894)
                     /locus_tag="ACG8M5_001227"
     CDS             complement(21998..22894)
                     /locus_tag="ACG8M5_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0358496.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23008..23178)
                     /locus_tag="ACG8M5_001228"
     CDS             complement(23008..23178)
                     /locus_tag="ACG8M5_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358497.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23175..23852)
                     /locus_tag="ACG8M5_001229"
     CDS             complement(23175..23852)
                     /locus_tag="ACG8M5_001229"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0358498.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23943..24848)
                     /locus_tag="ACG8M5_001230"
     CDS             complement(23943..24848)
                     /locus_tag="ACG8M5_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0358499.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25023..26477
                     /locus_tag="ACG8M5_001231"
     CDS             25023..26477
                     /locus_tag="ACG8M5_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0358500.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26493..27524
                     /locus_tag="ACG8M5_001232"
     CDS             26493..27524
                     /locus_tag="ACG8M5_001232"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0358501.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27531..28718
                     /locus_tag="ACG8M5_001233"
     CDS             27531..28718
                     /locus_tag="ACG8M5_001233"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0358502.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28874..30256
                     /locus_tag="ACG8M5_001234"
     CDS             28874..30256
                     /locus_tag="ACG8M5_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0358503.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30293..30613
                     /locus_tag="ACG8M5_001235"
     CDS             30293..30613
                     /locus_tag="ACG8M5_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358504.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30813..31481)
                     /locus_tag="ACG8M5_001236"
     CDS             complement(30813..31481)
                     /locus_tag="ACG8M5_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0358505.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8982 a         6422 c         5714 g        10588 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
     1741 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
     1801 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
     1861 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
     1921 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
     1981 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
     2041 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
     2101 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
     2161 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
     2221 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
     2281 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
     2341 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
     2401 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
     2461 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
     2521 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
     2581 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
     2641 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
     2701 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     2761 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     2821 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     2881 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
     2941 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3001 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
     3061 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3121 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3181 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
     3241 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
     3301 cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     3361 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc
     3421 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa
     3481 tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     3541 tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca
     3601 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
     3661 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
     3721 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
     3781 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
     3841 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
     3901 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
     3961 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
     4021 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
     4081 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
     4141 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
     4201 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
     4261 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
     4321 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
     4381 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
     4441 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
     4501 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
     4561 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     4621 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     4681 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     4741 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     4801 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     4861 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     4921 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     4981 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     5041 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     5101 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     5161 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     5221 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     5281 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     5341 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     5401 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     5461 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     5521 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     5581 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     5641 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     5701 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     5761 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     5821 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     5881 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     5941 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     6001 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     6061 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     6121 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     6181 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     6241 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     6301 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     6361 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     6421 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     6481 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     6541 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     6601 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     6661 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     6721 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     6781 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     6841 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     6901 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     6961 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     7021 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     7081 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     7141 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     7201 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     7261 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     7321 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     7381 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     7441 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     7501 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     7561 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     7621 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     7681 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     7741 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     7801 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     7861 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     7921 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     7981 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     8041 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     8101 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     8161 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     8221 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     8281 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     8341 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     8401 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     8461 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     8521 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     8581 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     8641 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     8701 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     8761 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     8821 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     8881 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     8941 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     9001 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     9061 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     9121 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     9181 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     9241 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     9301 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     9361 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     9421 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     9481 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     9541 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     9601 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     9661 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     9721 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     9781 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     9841 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     9901 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     9961 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
    10021 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
    10081 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
    10141 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
    10201 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
    10261 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
    10321 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
    10381 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
    10441 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
    10501 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
    10561 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
    10621 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
    10681 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
    10741 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
    10801 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
    10861 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
    10921 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
    10981 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
    11041 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
    11101 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
    11161 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
    11221 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
    11281 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
    11341 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
    11401 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
    11461 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
    11521 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
    11581 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
    11641 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
    11701 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
    11761 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
    11821 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
    11881 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
    11941 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
    12001 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
    12061 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
    12121 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
    12181 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
    12241 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
    12301 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
    12361 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
    12421 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
    12481 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
    12541 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
    12601 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
    12661 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
    12721 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
    12781 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
    12841 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
    12901 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
    12961 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
    13021 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
    13081 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
    13141 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
    13201 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
    13261 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
    13321 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
    13381 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
    13441 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
    13501 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
    13561 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    13621 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    13681 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    13741 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    13801 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    13861 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    13921 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    13981 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    14041 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    14101 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    14161 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    14221 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    14281 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    14341 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    14401 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    14461 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    14521 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    14581 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    14641 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    14701 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    14761 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    14821 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    14881 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    14941 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    15001 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    15061 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    15121 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    15181 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    15241 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    15301 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    15361 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    15421 tttcataggt aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt
    15481 aacatttaaa caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag
    15541 taatcgtttt tctatgtttt tttatttttg aatgaaaaac gcagattctg taacattcac
    15601 tgcaataatc cataaaaaaa agcaaaaaaa tttttgattt ttatcaaatt ctcttaaaaa
    15661 aatgtaaaaa atacaaaaaa accagtaatt gttacaacaa ttactggaca aaaactttga
    15721 ataatgttaa ctattttttt attttaactt tctccgcaat aatttctggg catgttccaa
    15781 ttcgtttata acgacttctt ctgtttcttt agctaatatt tcatctaata catcaagcca
    15841 tttttccggt tcttttgttc ctagacgtcc aatagcccaa gctgctgttc ctctgatgac
    15901 agggcggaca tctgttaaac attctgaaat ctcaggcaat gccgttcgat cacctagatt
    15961 agctaaagca atcaatgcgt ttctctgcaa aggtttcttc ccacgccatg aaccagctaa
    16021 atgcccaaat tgctgtttga agtctttatt agacatcgaa agcattggct ttaattttgg
    16081 atatatttcg tctacttttg gttccatctc ttcatgaaaa tgaaaatctt ttccttgatt
    16141 gtaagggcag actaactggc agatatcaca tccataaatg acatttcgca tttttttacg
    16201 atattcttcc ggcatcatcc cttttgtctg agtttgataa gacaaacatc gctgggcatt
    16261 cattcttccg tcaccaagca atgcttttgt cggacaagca gtcacgcagc gcatacagtc
    16321 accgcaacca aattctcctg gtttatcagg ctcaaataca atattagtaa cgatctcacc
    16381 aagatagaca aatgaaccga actcttcagt gatcaacaat ccgtttcgtc caataaatcc
    16441 taaaccagct cgctgtgcta cagcgacatc gatcagttct ccagtatcta cttgcggagc
    16501 tagacgccat tcctcttctt tttgccattt ttctgctctt gtttggatga attcgatcaa
    16561 tcggttcaaa cggtctctga gtatatcatg gtaatcgatc ccccaagaag cacgagcaaa
    16621 ctgcccgcgt ttttcatccc taggcacttc ttcatggatc ctagtaggat acgcaagtgc
    16681 aatggaaatg atcgatttcg gctgatcaaa tgtcatttct ggataaagac gctcatcgat
    16741 atttggatgt tcaaagccag aagtatggcc agctgctttt tgttcttcca acgattcttt
    16801 taatgaatca aaaggttccg ccgtggtgaa cccgattttg tcaattccta atcgtttgct
    16861 ttcttgtatg atttcttctt ttaatgtcat ggtcttcctc gcttgctgtt aacggctgta
    16921 taacccataa tatagttttt cttttttcag ctggacaaac tggatctgct gatcggcaat
    16981 cttcttttct tccatccatt tttttcttgc agcaaatact tcccaataat ttgttgaaat
    17041 taattttctt ttgttatcta atgcattaaa aaaattcaat gaaactggtt ttgtacttaa
    17101 cagttcatgg agccttacct cttcccatgg cgaaaactca gacagatcgc gggaatacaa
    17161 tacttcttca tagagatttt tttgctgtgt gtacgtgaat gtttgcggga ttttcccagg
    17221 cctagcaatt tctactagac gattataaaa catattcatt aatttgctgt tggcaataaa
    17281 ttgactttta taataggccg actggatctt tttgattact ccttctgctt tgatgactga
    17341 aaataatgca ggccatgctt tgacggttat ttcgtcgttt tgtttaacta atacgccagg
    17401 tgtgaagccg actggagaaa ggaacacaaa ataaccgata ccggtatttt catctaaagg
    17461 ctgaaattca tcaaacaacc atttgcctat gtacagctct tcatctggtt ctgttgtctc
    17521 attttgattt gctatgaata atttagctaa ttctgcttca agaaattgaa ggtcttgatc
    17581 cttgtcggta ttttcctgct ggctgatcaa tcgaaagact tcttctttaa tcgtatattt
    17641 atctttacgt gtaaaattca tagccacacc ttccttcctt actctaacta taacggaatt
    17701 tcttcaaaaa aggtatctgt acgacgaaaa acagcataat tttttttcat atctcttata
    17761 aaaaatgcaa ggtctgctcc tcaagctaga ccttgcactt ttcagctttc ttttgtttgt
    17821 gacaaacctt catggatcgt ttcaatattc catttcatca tctgatagta agaatctcct
    17881 tgttctcctt cttttgctat ggaatcagtg aacagtttat catagatttt caaacccgtt
    17941 tcttttgcta cccgttccat gcttcttgag tctacactgg tttccacgaa taaaaccggt
    18001 acttcttttg ctcttatctg atcaatgatc gctttcattt gatcaggcgt accttgactt
    18061 tctgtattga tctcccatat ataagctgct ggcaaatcat atgctcgtga aaaatacttg
    18121 aatgcccctt cacttgttac taataatttt ttgttctctt cgatagaagc aaaactttcc
    18181 ttcgcttgtg tatctaattc ttttagtttt tccacatatg cttctgcatt ttttttatag
    18241 agtgtcgcat tttctgcatc tttttcagag aatatacgtg cgatttcctc cacatatttt
    18301 attccgttag ataggtctag ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta
    18361 gttaaataaa gaggttctac atttttgcta actgcaaaat aatcttttcc ttcttctttt
    18421 ttagccgttt ccatcaagtt atcgaaccag ctgttacctg tttcaagatt caaaccgttg
    18481 tataaaataa catctgcatc gcttgccttt ttgatgtctt ctggtagtac ttcatattca
    18541 tgcggatctg ttccgactgg tacgatactg tggatatcta tttgatctgt tccgacctct
    18601 tttgccatgt ccgccaagat cgaattggta gccacgactg acaacttttc tttacttcct
    18661 tcatccgctt ggtcagaagc ctgctttccg caaccggcta ataccaagaa actaactgcc
    18721 aagattaaaa agagtgattt ttttgttttc atctatctgc ttcctttctg aaataaaatt
    18781 ccttttgttg gagaaaataa aaaagcaagg ataaaaaatg cggtacatgc taacacgatc
    18841 gtagctcctg atggccaatt gtaactataa ctgaaaaaca caccgacaac tgcacttagc
    18901 ataccgattc ctgcagctgt aatgatcatt ttcagtaaat ggttcgttag caagtaagcc
    18961 gtggcggctg gtgtgatcaa catggcaatc accaagattg ttcctactgt ctgtaaactg
    19021 actacagcca ctaatgtcaa aaagaacatc aaaagataat ggatcagcga tgtgttcagc
    19081 ccataagctt ttgccattgt cggatcgaat gatgtgatct tcaattcttt ataaaagaaa
    19141 aagacaaata tcaagacgat tccactaacg agtgctgtca gcaacaaatc gctttcccta
    19201 acagctaaga cattcccaaa taaaatatga tacaaatctg tcgcactctg ggcatatgaa
    19261 atcaagataa tccctaatgc aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt
    19321 ttcaatcggc tccgctgagt cacgaaacca atcactgctg ctgttagcat cccaaaagca
    19381 gtggctccaa agatgtaact aaaaccaaac atataagaaa tggctacacc tggaagaact
    19441 gcgtgagaga tagcgtctcc catcaaagac atccctcgta aaataataaa agaaccgatg
    19501 actccagaag caagtcctac aagcatggaa gtgaccaaag cgtattgcag gaattgataa
    19561 ttcattaatc catcaataaa ggattggatc attttttctc tcctcctttt actaaatgtc
    19621 tgatgatctc accataggcc aattgaaggt tcttcgatgt aaagacttct tgtactggac
    19681 cggaagcgat cagctgttta tttaaaagaa taacctcatc gaaatattct tctactttat
    19741 gaagatcatg atggacgatc aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt
    19801 caaagatctt tcgttcactt aacgcatcaa tccctacgaa tggttcatct aaaaagatcc
    19861 attctgctcc ttgggctaga gctcttgcaa taaaaactct ctgaagctgt ccacccgata
    19921 gttcgctgat ctgtcttttg gcatattctt ccatccctac ttttttcaaa gcttgtcttg
    19981 cacgttcttt ttcttttttc ccaggtcttt gtccaattcg taaagatgga tatgttccta
    20041 aaagtacaac gcctaataca tcaattggaa aggaaagatc caattcactt cgctgttcca
    20101 cgtaggctat ttttttacgg actgagttta ccgactgtcc agaaaaggtg actttaccag
    20161 tagtagctgg tatcaactct agcaaagatt tcataaatgt tgattttcct gcaccgtttg
    20221 gtccgataat accggtaatt ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag
    20281 ttttcccttg ataagcggct gaaaggtctt gaattacaat ggccgtacgt tccaaatctt
    20341 acactccttt ttaggtttac ctaacttttt atttaaatat agtttaacta aaatggaaag
    20401 cattttcaag tagaatgatg aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat
    20461 tttctgtaga atcacatcaa gagttcaata aggctaacct aataatgaaa aacatgtgtt
    20521 gaaacgtttg attatttttt cttgttgact caacaacgaa aggagttttt gtatggaaga
    20581 agaaaagtta actcgaagtc ttagtgcccg tcatatccaa atgatcgcac taggtggaac
    20641 gatcggtgtc ggtctgttta tgggtgcctc ctcaaccata cgttggacag ggccctcagt
    20701 gatgggagcc tatgcgattg caggattctt tttgtatttg attatgcgtg ctttaggcga
    20761 aatgctttac gttgatcctt cgactggttc ttttgcaaat tacgcgagcg agtacatcca
    20821 tcctgtggct ggctacttga ccgcttggag taatattttt caatatattg tcgtaggaat
    20881 aagtgaagtg attgctgtag gatcttatat gaattattgg tggcctgatt tgccggcaat
    20941 tattcccgga atcattgtcg tcttgtttct tatgctggca aatcttattt ctgtcaaggc
    21001 gtttggcgaa ttagaatttt ggttttcgat catcaaagtc ataacgatca ttttgatgat
    21061 cattgcaggt cttggggtaa tcttatttgg atttggtaat catggtcagg cagtagggat
    21121 ttcaaatctt tggaaaaatg gtggattctt cactggaggc gtcaaaggat ttttctttgc
    21181 attatcgatc gttgttgctt cctatcaagg aatcgaactg atcggaatga ctgctggtga
    21241 agcagagaat cctaaaaaga cgatcattga agctgtccag tccactatcg gtcgaatctt
    21301 gattttttat attggtgcta tttttatcat tgtgagtatc tatccgtgga atcaattgag
    21361 tgagattggt tcaccttttg tacaaacttt ttctaaagtc ggcattacat ttgctgcagg
    21421 actgattaat tttgtcgtga ttacagcggc attatcaggt tgcaattcag ggattttcag
    21481 cgcaagtcgt atggtctata cacttgccat aaatggaaag atgtctaaaa aatttctgaa
    21541 actatctcgt cacggtgcgc ctttttatcc tgtcgtagca atctcaggtg gaatcttaat
    21601 tggtctgatc ttaaattatc ttctaccaat gttcatcaac cagtcagaaa atattttcgt
    21661 tttcgtatac agctcaagta ttttgcctgg tatggttcct tgggtagtga ttttgatcag
    21721 cgagattcgc ttcaaaaaga agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc
    21781 actatctcct tatacgaatt atgtgacact tgctttttta gctatcgtat tgattttcat
    21841 gtttatcaat cctgaaacga gaatctcatt gattattgga ctcgttttct tgctttacat
    21901 gacgatccat tactttgtac gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc
    21961 accttagtgg aagcttcgtt cttttttatt gcttttatca attcaatttt agcgtctcac
    22021 cgggagtcag gaaataattc tccatgctca tcccattcag cgacaagatc gtttctactg
    22081 gtacgccagt tcgttcggct atttgatttg gaccttctcc ttccaaaact acggttgtcg
    22141 cagcctgtga agtcgcgctg ttggtattag tttcagagga atctgttgtg tcttgtgttg
    22201 ttcgagcttg catttcagaa gcttctaccg actctgctgt ctcagaatca gaagatgttg
    22261 tgtcttgtgt gcttgttgac tctgcttgaa gagaggcgct agtctgcaaa taaatatcgg
    22321 aaatcttagc gacatgattc gtataattag ccgataattg attgatcaaa gtatctaagt
    22381 ctacaccgtc tttattttct gctgcctgat acgctgcacg taattttctg atcccttcgt
    22441 cggagattgc ttgaagctca cgtgcttttt ggttagcaac tgctgataaa ccagctattc
    22501 cctcttgatt tccctgaatt tctgtctggt attcttcaat caatgcaggc gtcttttctt
    22561 cgatcttttt cgaataatca tttacgattg cttgaagttg ctctggcaaa ccatttgctg
    22621 ttttttcttc agaagtttgt gtctcttttg ttttagttga tgactcactt gtttgtttcg
    22681 tctctgtttt ctttgtcgtt gtttccgtta tggtcgtact tttcgcttct gacgaatttt
    22741 ttccatgaaa atatagaccc gcaactcctc ctgctatgat caaaaacacg gcaactgctg
    22801 ccccaatcat gatcatccgc ttacgagctg cctgtttttg ctgtcttctt cgggtcatcc
    22861 gaacattttc tccccactca ttttcatcat acattttttt caccaccaat ttttatgtat
    22921 aatagcgtta tcaaacgctt catattctta ttgtagcaaa taattgtcgc tgtgcacatt
    22981 tttatctttg ttatttataa gaaaatttca ccagttgaga tgattccaag attgtccgtc
    23041 gatttctcct tgcagctgca ttgttcgttc gtctatctca gccatcagtt tctttatttc
    23101 atataagagc gcacggtctt gatattctga tttttcgctt aattcatcta attgttggat
    23161 cagccaatca tttgtcataa tgaagttcct ttcatttctt gcagtgtaaa ggtcaagtct
    23221 tcgatctgct cttttgcctc agtcagtgct tctacgatat atgcttgttt ttcagattct
    23281 tcttcctgca ttttctgatt ttcctgtact ctttgaataa accagtcaat ccgctgagtc
    23341 agcgatggtt tgccttccaa tgagagatac tgctgataag caattagctt ctcccaacgt
    23401 ttttcctggg ataaatacat aaattgtgct aaaacgaatg gcggaagata ggtcatatgg
    23461 aatttatttg cgattcgctg atacggcctc aaaaattcgg aaatcgtata ttcttctttt
    23521 cctccagctt ggtattcttt ttccgccaca cctgttgtca atacgattcc tagttctttc
    23581 ccttttaata catgtgctgc tcgttccaag acattatctt gccaaatttt taaatgagca
    23641 ggagaactat accagtaaaa cggaaactgg aaaatgatcc gatcatgctg ggccaagagc
    23701 tgtagttcag tctctatatc tatttttccg tctggataac ttgattccaa atgatgccaa
    23761 gtagctggta aaccagcaga agcttctttg aaaaattgct gatttaaaga ttcttcgatc
    23821 gttggatggc taatgatgat aagtgtacgc atcccttttc ctccttttta gtcttcgctt
    23881 tacagtgtag cataaccgcc acaagtttct tttgccgatg cttgtcattt taaccagact
    23941 aatcaggttg tccaagaaac tgcggtttgt ttttccaact gttttttcaa ggtatccaaa
    24001 gcaatattct taaaattttc gtttttcata cgtttacgat agttcgtagc cgtttctccg
    24061 gtactctttt tgaacattcg gctaaatacg actagactat tgaaccctac ttgataggca
    24121 atactttcca cggattcatc cgtcagagaa agaagaaacg cccctcgatt gatccgtacc
    24181 ccgatcaaat attccttgat actggtaccc atatgatttt tgaatactcg cgacaaatgg
    24241 ctccgattga ccgctaaatg ctgggcgatt tcctcaacag acaatttttc agcataatgt
    24301 gcctcgatgt atttgactgt ttcaacagct aaagaggaat gaaccttttg tacaccaccg
    24361 ctgacctttc cgccatcttt taaaaggact gataaaaaac ggtaggttaa tgctgttaac
    24421 tgtaattcat tttccaaggt ttcttccgta tctttcatgc attctaaaat aatgtcgata
    24481 tatttttgta tgtcagaaga gaccatcgtg tattggtctt ctctgaaaag cgactgccga
    24541 atgatttctt ctgcagcact cccaccaaat gaaagccagc aatacatcca cggatcttct
    24601 ccatctgata agtaaaacgt cgagtctcct ggacgaatca agaacagatc accttttttc
    24661 aattggaatt gttgattttt cacatgataa gatcctttgc cttccatcac gacatgcagg
    24721 atgtaatcat tgcgaatagc aggaccaaaa gaatgaaacg gcatcgtttt agagaaacca
    24781 caaaaatcaa aatacaattc tttttctact tgttgatgtt ccttttttaa aaaaatcgat
    24841 tgctccacgt ctatccctcc ttactccttt agtataatgc aacaaaaagt taaataaaag
    24901 tcacagaaat atattaaaaa ggaagcgatt tcttgttaag tttaagtcat taaaagatct
    24961 ttgttattcg ttgtaaccgg tttacttagt attatatatt gaacaggaga ggaatacaga
    25021 ttatgaaaag caaaatgatc caacgtgtgt cctacgcttt aggcgctttt ggacacgaca
    25081 cattctatgt tacattaagc acttatttta tggtattcgt gacaagccag atgttcgcag
    25141 gaacagataa ggcaacgaat gcaaaaatga ttgctacagt tacaagctta gttgtaggta
    25201 tccgcttagt tgaaattatt tttgatcctt tgattggtgg catcatcgat aatactcgta
    25261 caaaatacgg taaattcaaa ccatggttag ttatcggcgg cgttgttagt tccgtactct
    25321 tggtagcaat ctttaccaat ttctttggct tagctactca taatacgact ttatttatca
    25381 tattatttgc aatcgttttc atcttattgg atagttttta ttcatttaag gatatcgctt
    25441 tttggtcaat gattccagct ttgagtacgg aaacaaagga acgtgaaaaa tttgctacgt
    25501 ttgctcgttt cggctcttct ctaggtgcaa atggtactac tctagtcgtt gtccctatcg
    25561 ttactttctt tacctatctt gttactcata aacatattga aggcgcttca ggctggttct
    25621 ggttcggtgt gattgctgct gttatttcag gcggtactgc gattatcact gccttgggca
    25681 caaaagaagt cgatactgcg atccgtcagc aaaatgaaaa agctgggatc aaagatgtgt
    25741 tcaaagcagt tgctcaaaat gatcagttga tgtggcttgc ccttgcttat atcgcttatg
    25801 cgattgccaa tgcagcaaca actgccgtat tattctactt cttcaaatat gtacttggcc
    25861 aaccaactga attttggatc gttggtctag ttgctgcctt acttggttta gtagctgttc
    25921 cactattccc aatgcttaca cgcgtcatct ctcgtcgcta cgtttacctt ggagggatcg
    25981 caatgatgat ttcttcttac tttttcttta cgatcgcagg ttcaagtttg ccaatcgtta
    26041 tcatcgggct agtattgttc tacttccctc aacaattgat tttcttatcc gctttgatga
    26101 cgatcacaga ctctgttgaa tatggtcaat ggaaaaatgg tatccgtaac gaagctgtta
    26161 ctctatcaat tcgcccttta ctagataaaa tcgctggtgc tttgtctaac gggatcgtag
    26221 ggtttgtcgc tatcgcttct gggatgactg gtaatgctac tgctgctgat atcactgctc
    26281 atggcgtaac gactttcaaa gcttatgcat tctatgctcc tgctgctttg atgattttct
    26341 ccgctattat ttttgcttgg aagattacat tgacagagaa aaaacatgca caaatcgttg
    26401 agaaattgga aaacaaattg gctccagctg atacaatgga agatgaactt cacgatgcag
    26461 tagatccttc ccattaagaa aggatcttga aaatgaagat tacagaaaaa gattttggcc
    26521 aaggttatca cttgataacc ttggagaaca aaaacaagct agcactttct atctctgatt
    26581 taggtgctag aatcgtcagt ttaaaatcaa atgatcgaga attagttttg ggatttgaca
    26641 ccgctgaaga atatatcgaa aaagatccgt atatcggtgc ttctatcggt cgcaccgcag
    26701 ggagaatcga aaatggccgc ttttctctca atggaaaaac ttaccaatta gccacagatc
    26761 caaaaactgg acataatctg catggtggtg ctcctggatt tgaattaaaa aaatggtctt
    26821 atgtcatttt gaatggcgaa aatgaagctt ctgtcatttt tactactaca agtcccgacg
    26881 gagaacatgg ttttccaggt acaatggatg tggaaatccg ctatactttg acaaaagata
    26941 atatctggcg agtgacctct cgcggcacca gtgaccaaga tactttattc aatccgacaa
    27001 atcatgttta cttcaacttg acgggagatg cttctcagtc aatcgatcaa catgagcttt
    27061 ggctcaatag cgaagcttat gctcctttgc gaacagatag tattcctata ggggtaaaag
    27121 aaaatgctgc aggaagtgct ttcgattttc aaatacctaa aaaattagcg tccgtttttg
    27181 ctagtgattt ggatcaaaaa aatcttgttg acggaatcga tcatccattt ttcttaaaag
    27241 aaaccggctt gggtaaagaa gccgctcgct tgactagccc cgataaaaga atccaagtag
    27301 atattgctac agatgcttct agtgtggtta ttttcactgc aaatttcggc acagaaacac
    27361 cagaaatgcg caaccgaaaa ctggcacatc acggtggcat cacatttgag actcaaacag
    27421 ctccaggtgc agagcgcttc tcagcatttg gttcgattca tttaaaagcc ggatcagtat
    27481 ttgaaacagt aacagaattc aaaatcaaaa caagaaagga ataactcatc atggaaatca
    27541 aaagaagctt aaacacaaaa tttgccgaaa cattcggacc taaaaaaact acacaatact
    27601 tctcaccagg acgcatcaat ttgattggtg aacacacaga ttacaatgga ggccatgtct
    27661 tccccgcttc catcacttat ggaacgcaag gtgtagcagc acctcgagac gataataaaa
    27721 tattagttta ttccacaaac tttgacgata tcggtgtcat tagttttact ttagatgagc
    27781 tagaatatga taaaaaagca aactgggcaa attacgtaaa aggaatggtc cttaaattaa
    27841 aagaagctgg acactcaatc gatcatgggt ttgagttatt agtggaagga acaatcccta
    27901 atggtgcagg actttccagt agcgcttcat tagaattatt agtaggtgtt gtattagaag
    27961 atctgtttga tttaaaaaca gatcgcttgg aactagtcca aacaggtaaa aaagtagaaa
    28021 atgaattcat cggtgtcaat tcaggtatca tggatcagtt tgccatcggc ttcggtgagc
    28081 tagatcatgc aatcttatta gatacaaata cgctgaaata cgaaatggtt ccagtgaaac
    28141 tagatggtta tgcaatcgtc atcatgaata cgaataagcg gcgggaattg gcagattcaa
    28201 aatacaatga acgccgtagc gaatgcgaag aagccttggc acgtttgcaa aaaactttgc
    28261 ctatctcttc tttaggagac ttagatgaag aagaatttga atcaactatt gaccaaatcg
    28321 gcgatgatac attgatacgc agagctcgcc atgccgtttc tgaaaatcag cggactttga
    28381 aggcaaaagc tgaattagaa gctgggaact tagaagcatt tggtcaactg ttaaatgatt
    28441 ctcaccactc tcttcgctat gattatgaag taactgggat cgaattagat acactagtag
    28501 atgctgctca aaaacaagaa ggtgtcttag gtgcacgaat gacaggagct ggatttggtg
    28561 gctgtgcgat tgctcttgtc aaagaagaaa atatacctgc cttcaaaaac aatgtctatg
    28621 atgaatacat gaaagttgtc ggctatgctc cggaatttta tgttgcacac attggaaatg
    28681 ggacaacaaa gatcgatcca gaagctgttc atgtttgata aaaaaatcaa cgatctcagc
    28741 taaaaacaaa tagataaaaa taacctctca gcaatttcca aatgtgagcc ttttgctcgt
    28801 atttgaaaat tgctgttttt tattgacacg gaaagagaac gtgtgttcta attgtgttga
    28861 agaggtgatt gttatgtggg cgaatcaaaa tccggtattt gtttatgaaa aagaaccttc
    28921 aagagatatt ttatgtattg actgcaaatc tttttacgcc tccgtagagt gcgttgaaaa
    28981 ggatcttgat ccccttacaa ctaaattagt agtcatgtcc tatccgtccg attcgacaga
    29041 aacacggggt agcggattaa tcttggcttc cagtcctgca gcaaaaaaag cttacggcat
    29101 caccaatatc agccgtgcca gagatcttcc cttcccctat ccaagtgatt tatatattgt
    29161 tgccccaagg atggcctatt atatggaaaa gaacagagaa atcaattcta tctataaaaa
    29221 atatgcagac gaagcgaacc atcatgtcta ttccgtcgat gaaagcttcc ttgatgtaac
    29281 aaatagcatg gcactctttg gtaaaacaaa tgcgtacgac atggctaaac tgatccataa
    29341 agatgtgttt gagcagaccg gaatctacac gactatagga attggcgata acccgctact
    29401 tgctaaacta gccttagaca atggctcaaa agacggaaag caaatgtttg ctgaatggcg
    29461 ttatgaagat gtaccagaaa agttatggaa aatctcagat atcactgact tttggggaat
    29521 cggcaaacga acagcaaaac gtctgaaaca aatgggtata cattctgttt atgatttagc
    29581 tcacgcggat tattatcact tgaaaagcaa gctaggggtc atcggtactc agctttatgc
    29641 ccatgcttgg ggcatcgatc gaagtttttt gggacaagaa tatacaccaa aatcaaaaag
    29701 tatcggaaat agtcaaatcc tagatagaga ttatacaaga cgagaagaaa tcgagatcgt
    29761 tatcaaagaa atggccgacc aagtcggcac tcgcttgaga cgatctgcag caaaaacaca
    29821 agtagtcagc ttatgggtag gatactcttt aggctatctt gatccaaatg gcaagaccgg
    29881 gtttcaccag caaatgaaaa tcgaagcgac aaatgctagc agagatctag cagatgcttt
    29941 attgaccatt ttccaccagc attaccacta ccaggatatt cggcgtgtag gcgtgaactg
    30001 ttccgacctc gtatacgcga caggtttaca gcttaacctt tttgaagatc ctgaaaagca
    30061 gataaatgaa gcgaaagttg attttgttgt cgatacgatt agaaaaaaat acggctttca
    30121 atcaatcatc catgcgcata gtctcttgca aggaggacgc gccatcgcac gtagttctct
    30181 cgttggcggg catgcaggtg gtatgagtgg attagaaggt ggaaaagatg agacggacag
    30241 caaaaaaatt cattgactat aacgagtatc gagatcggcc atttggatta aaatggggga
    30301 ctgcttttgc gatggaagaa ctagtcaacg gtatcaaaaa aatgaagaag aagcacgcaa
    30361 acagcctctt cctaaacaac aaatgacatt gcaagaaatc gatagtatac tgacagaagc
    30421 ctttttatct cgtaaagcag taatcattca gctcaattta aaagatgaat ttggaagact
    30481 cttggatgag gtcgaagggc acttcattgg tgaagcctat gaagattact ttatgattga
    30541 ccatcagccg atattgtggg aagacgtacg gtatattgag ttaaaggaaa caaaaaaatg
    30601 gtttgtgaca tgagaggttg tgacagaagt gatcaacttc aaaaaataag aaaggatttt
    30661 tcgaaaattg cttcttaaat ttttgtggaa ttttggctta tttttcgaag aagcaacttc
    30721 tggaacaacg tatattagat atcagaggtt gtgaaaaagt cgcttagttt tgagtaacaa
    30781 gccaaggcgc tgatttcaca acctctgtct atctatccat ctattcggta agattcatgc
    30841 atgacgtctt ctttatttag tatgtagagc cctttttctt ctgaccattc aacataaatc
    30901 agctgtttta catctgcata gccttctttc ttcaagttct ccataagcca tgcttcagtc
    30961 aattgattgg actgaagcac tttatgctga atttgccctt catctacaag caggatcgat
    31021 aaagctttgt cttcatctga ttttttcaat acactcattt gaccggcatt ctctaggata
    31081 acatgtttgg cattttcaag aaaaaagcat tcttgctgtc ttaagattgc tcgcaactgc
    31141 tccatctcga tatggttatt tgtcaactct gtcatgttga tgacgccgtc tttgattaga
    31201 acagatggct cgcctttgac ccaacgattg accttttcat ttttttggac gatacgttcg
    31261 ataccataaa tcattactgc ccaaagcgca atggcaaata ggacatgccc tacatggaca
    31321 ttgtcatcat aggttgattc ttctaagatc ccacctaaaa caagcgtata gactaaatca
    31381 aacggtgtga tttctgacat ggatttcttc ccaagcaaac gtaccactaa taataaaccg
    31441 atcaacccga acaccagttt cattgtaatt gaaaaaatca ttttttctcc ccctaactat
    31501 ctgttaatta aattatatat gctttctcac gaacacgcat ggaatataca aggaagaaat
    31561 gtatcctttt actaaaaaaa ggactgtgac actgtagtga cccccaaaag ttagactttt
    31621 ttggagtgga gaaatccact cctttttttt atgctctaaa atgatatttt tacacaaccg
    31681 ttacggttgt tttacgaaat tggacg
//
LOCUS       DAZKMZ010000024        30459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000024 DAZKMZ010000000
VERSION     DAZKMZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.024"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8M5_001237"
     CDS             complement(451..1761)
                     /locus_tag="ACG8M5_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0358506.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG8M5_001238"
     CDS             complement(1773..3131)
                     /locus_tag="ACG8M5_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0358507.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACG8M5_001239"
     CDS             complement(3333..4901)
                     /locus_tag="ACG8M5_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0358508.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACG8M5_001240"
     CDS             complement(4905..5315)
                     /locus_tag="ACG8M5_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0358509.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACG8M5_001241"
     CDS             complement(5312..6235)
                     /locus_tag="ACG8M5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0358510.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACG8M5_001242"
     CDS             complement(6232..7110)
                     /locus_tag="ACG8M5_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0358511.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACG8M5_001243"
     CDS             complement(7097..8434)
                     /locus_tag="ACG8M5_001243"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0358512.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACG8M5_001244"
     CDS             complement(8437..9540)
                     /locus_tag="ACG8M5_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0358513.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACG8M5_001245"
     CDS             complement(9614..11122)
                     /locus_tag="ACG8M5_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0358514.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACG8M5_001246"
     CDS             complement(11088..11780)
                     /locus_tag="ACG8M5_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0358515.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACG8M5_001247"
     CDS             complement(11802..12776)
                     /locus_tag="ACG8M5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358516.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACG8M5_001248"
     CDS             13076..13948
                     /locus_tag="ACG8M5_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358517.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACG8M5_001249"
     CDS             complement(14406..16604)
                     /locus_tag="ACG8M5_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358518.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACG8M5_001250"
     CDS             16836..17828
                     /locus_tag="ACG8M5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358519.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACG8M5_001251"
     CDS             17888..19309
                     /locus_tag="ACG8M5_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0358520.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACG8M5_001252"
     CDS             19302..20801
                     /locus_tag="ACG8M5_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0358521.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG8M5_001253"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG8M5_001253"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0358522.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACG8M5_001254"
     CDS             22393..23076
                     /locus_tag="ACG8M5_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0358523.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACG8M5_001255"
     CDS             23078..23914
                     /locus_tag="ACG8M5_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0358524.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACG8M5_001256"
     CDS             23925..25679
                     /locus_tag="ACG8M5_001256"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358525.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACG8M5_001257"
     CDS             complement(25955..26296)
                     /locus_tag="ACG8M5_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0358526.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACG8M5_001258"
     CDS             complement(26364..28535)
                     /locus_tag="ACG8M5_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0358527.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACG8M5_001259"
     CDS             28746..29603
                     /locus_tag="ACG8M5_001259"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0358528.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..>30459)
                     /locus_tag="ACG8M5_001260"
     CDS             complement(29674..>30459)
                     /locus_tag="ACG8M5_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0358529.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9099 a         6274 c         5391 g         9695 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaattt
//
LOCUS       DAZKMZ010000025        29593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000025 DAZKMZ010000000
VERSION     DAZKMZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.025"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..1125)
                     /gene="whiA"
                     /locus_tag="ACG8M5_001261"
     CDS             complement(190..1125)
                     /gene="whiA"
                     /locus_tag="ACG8M5_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0358530.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1159..2157)
                     /gene="yvcK"
                     /locus_tag="ACG8M5_001262"
     CDS             complement(1159..2157)
                     /gene="yvcK"
                     /locus_tag="ACG8M5_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0358531.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2154..3038)
                     /gene="rapZ"
                     /locus_tag="ACG8M5_001263"
     CDS             complement(2154..3038)
                     /gene="rapZ"
                     /locus_tag="ACG8M5_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0358532.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3173..3904)
                     /locus_tag="ACG8M5_001264"
     CDS             complement(3173..3904)
                     /locus_tag="ACG8M5_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0358533.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(3908..5173)
                     /locus_tag="ACG8M5_001265"
     CDS             complement(3908..5173)
                     /locus_tag="ACG8M5_001265"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0358534.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5436..8252)
                     /gene="uvrA"
                     /locus_tag="ACG8M5_001266"
     CDS             complement(5436..8252)
                     /gene="uvrA"
                     /locus_tag="ACG8M5_001266"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0358535.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8262..10256)
                     /gene="uvrB"
                     /locus_tag="ACG8M5_001267"
     CDS             complement(8262..10256)
                     /gene="uvrB"
                     /locus_tag="ACG8M5_001267"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0358536.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10515..12017
                     /locus_tag="ACG8M5_001268"
     CDS             10515..12017
                     /locus_tag="ACG8M5_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0358537.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            12019..12756
                     /locus_tag="ACG8M5_001269"
     CDS             12019..12756
                     /locus_tag="ACG8M5_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358538.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12927..14120)
                     /locus_tag="ACG8M5_001270"
     CDS             complement(12927..14120)
                     /locus_tag="ACG8M5_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0358539.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(14199..14405)
                     /locus_tag="ACG8M5_001271"
     CDS             complement(14199..14405)
                     /locus_tag="ACG8M5_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0358540.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(14398..15540)
                     /locus_tag="ACG8M5_001272"
     CDS             complement(14398..15540)
                     /locus_tag="ACG8M5_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0358541.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            16052..16687
                     /locus_tag="ACG8M5_001273"
     CDS             16052..16687
                     /locus_tag="ACG8M5_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0358542.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            16841..16918
                     /locus_tag="ACG8M5_001274"
                     /pseudo
     CDS             16841..16918
                     /locus_tag="ACG8M5_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17232..17279
                     /locus_tag="ACG8M5_001275"
                     /pseudo
     CDS             17232..17279
                     /locus_tag="ACG8M5_001275"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17295..19214
                     /gene="tet(M)"
                     /locus_tag="ACG8M5_001276"
     CDS             17295..19214
                     /gene="tet(M)"
                     /locus_tag="ACG8M5_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0358543.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            19333..19500
                     /locus_tag="ACG8M5_001277"
     CDS             19333..19500
                     /locus_tag="ACG8M5_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0358544.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(19560..19913)
                     /locus_tag="ACG8M5_001278"
     CDS             complement(19560..19913)
                     /locus_tag="ACG8M5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358545.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            20417..20836
                     /locus_tag="ACG8M5_001279"
     CDS             20417..20836
                     /locus_tag="ACG8M5_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0358546.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            20862..21068
                     /locus_tag="ACG8M5_001280"
     CDS             20862..21068
                     /locus_tag="ACG8M5_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358547.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(21296..21469)
                     /locus_tag="ACG8M5_001281"
     CDS             complement(21296..21469)
                     /locus_tag="ACG8M5_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358548.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            21481..22110
                     /locus_tag="ACG8M5_001282"
     CDS             21481..22110
                     /locus_tag="ACG8M5_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358549.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(22192..23358)
                     /gene="dnaJ"
                     /locus_tag="ACG8M5_001283"
     CDS             complement(22192..23358)
                     /gene="dnaJ"
                     /locus_tag="ACG8M5_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0358550.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(23509..25338)
                     /gene="dnaK"
                     /locus_tag="ACG8M5_001284"
     CDS             complement(23509..25338)
                     /gene="dnaK"
                     /locus_tag="ACG8M5_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0358551.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(25389..25952)
                     /gene="grpE"
                     /locus_tag="ACG8M5_001285"
     CDS             complement(25389..25952)
                     /gene="grpE"
                     /locus_tag="ACG8M5_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0358552.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(26046..27089)
                     /gene="hrcA"
                     /locus_tag="ACG8M5_001286"
     CDS             complement(26046..27089)
                     /gene="hrcA"
                     /locus_tag="ACG8M5_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0358553.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(27323..28488)
                     /gene="hemW"
                     /locus_tag="ACG8M5_001287"
                     /pseudo
     CDS             complement(27323..28488)
                     /gene="hemW"
                     /locus_tag="ACG8M5_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(28504..28731)
                     /locus_tag="ACG8M5_001288"
     CDS             complement(28504..28731)
                     /locus_tag="ACG8M5_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0358554.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            29215..>29593
                     /locus_tag="ACG8M5_001289"
     CDS             29215..>29593
                     /locus_tag="ACG8M5_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFQ0358555.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLES"
BASE COUNT         8497 a         6056 c         5337 g         9703 t
ORIGIN      
        1 actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa aaaacaatag
       61 aagcctttca acattatgtt actccagtaa agttggaatt atagtttgac ggaagaaaag
      121 caagtatagc ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg tatcagtttt
      181 ttctttggat taaactgctt cttcacgcag tttttccgca aattcattta ttttgcgaat
      241 tcggtgatta attcccgatt ttgatattgc acctgaggga atcatttccc ctaattcttt
      301 caagctgacc tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag
      361 ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc
      421 atcaatcgtc ttgttcagat ttgctgtctc acaattgact agacgattga ccgaatttct
      481 catatcccgt acgattcgaa catcttcaaa tcggagcatt gagttggttg caccaatcaa
      541 tgttaagaaa tcagcaattt tttctgctcc ttttaaatac gaaatatagc cattgcgccg
      601 ttcaagcgtt cgagcatgta gaccgtaata atttaacata tcgcaaatgt cttggttatg
      661 ctgttcataa atagaaaata tttccaaatg ataacgactc gtttccggat tattgactga
      721 acctgaggcc ataaaagcac cacgtaagta agaacgcatt ttttgcgaat ttcccataat
      781 ctcgtccgaa acgtgtccat tgaacatcat accatccatg atatcaagat ccatcaacac
      841 atcttttgtg ccttgcttca accggacgat atagacattg ttctttttta atttcatttt
      901 tttacgaacg agtagctcac ttcgtacctg ataatgatct ttcaaaagag aatagatacg
      961 tctggcaatt gcagcatttt ctgtttggac gttcaaaata aactgctggt tcacaatacc
     1021 taaagagccg ttcattcgca gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac
     1081 ttccaaacca gttagttctt tcttcacatc tgcagcaaat gacatcctat tctcctcctt
     1141 tctccaacaa taaattgctt aatattttga accgaaaact aaacggaaca attcttctac
     1201 tactttgtct ccatcgtgga agacaccacc atctttcaat tctaaaaagt cggtggaaat
     1261 cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc
     1321 atcatagatc tctgggtcca tataatcttc aggcactttt tccgtattga ccaatactgt
     1381 atcgataaaa ggctgattca aatgcttgtg taaaacacgt atatgatcgg catctgtgaa
     1441 atgttccgtt tccccttttt gggtcatgat attacagata tagacggttt cagcagttgt
     1501 cttgagcatt gcttcgccga tttctggtat cactaaattt ggaaggatac tggtgaataa
     1561 acttccgggt cccaaaacga ccatgtcggc gtccataatt gctgaaacga cttttcgagc
     1621 cgctttggct tcacttttat catttgtgtt gcagacatac acacggtcaa tggttttacg
     1681 gttgatcgcg attttagatt ctcctactgc agtcgttccg tctttaaaaa cagcatgcaa
     1741 ggtcaaagga gtttctgaag aaggataaac atgtccatct acatgcatca tttttgctaa
     1801 taattgaatc gcctcataag tgcttccccg catctcggaa actgcggcaa tgatcagatt
     1861 accgatcgta tgattggcta aaaactgatc ttcctttttg aaacggtatt ggaaaatatc
     1921 ttcatagaat tgtggcatat ccgataacgc cactaagaca tttctcaagt caccaggagg
     1981 agccatagcg atcgattctc tgattgcacc gctgcttcca ccatcatctg caacagtcac
     2041 tactgctgtg atatctgctc cttgatttcg taaacttttc aggataactg gcagaccagt
     2101 gccaccgccg accactacga tctttggttt tcttatccgg taggttttca ttttcacgac
     2161 cggttcaccg tctctttcct tttatccttg tcccgatgtg tcacattgac tttatagttt
     2221 ttccctaaag catttgccaa acgttcagtc aatgccacag aacggtgttg accgcctgta
     2281 cagccgattg caactgtcaa gctgcttttt ccttctttaa tgtatccagg caatacagtt
     2341 tccagaagat ccaaaaattt atggtagaat gattgcgttt catcaaagga catcacataa
     2401 tcatagactg gctgatcttt tccggtcaat ggtcttagtt ccgggatata atgagggttg
     2461 ggcaaaaaac gcacatccat cacaatatcc gaatcgatcg gcaacccgta tttaaaacca
     2521 aatgatacaa attcgatccg aaatccttgg tctccctttg atttgaatga ctcattgatt
     2581 ttttcacgta attgtcgtgg tgtcaatgtt gtcgtatcaa tgatcacgga agcttgggct
     2641 ttcaagtcgt ctagtatcgc ccgttcttta cgtatccctt cagtgatcaa accgtccatc
     2701 gccattggat gtgctcgtct tgtttctttg tagcgagcca ctagttcttc gtctgaagca
     2761 tctaaaaata agatagaagt gtcgatgaag ttcgtatttt cgatttcgat cagcatgttt
     2821 tggatttctt caaagaagga acgagaacgc aaatccactc ctaaagccat ttttgtgact
     2881 ttacctgatt ctttgatcaa ttcccagaat tttggaatca atcgaggcgg catattatcg
     2941 atacaaaaat agcccatatc ttcaaaactt tggatagcta ctgttttccc tgcaccactc
     3001 atacctgtta tgatgactaa ttgcaaatta tctgtcatat ttacgaaccc ctctcacatt
     3061 gtgcataata aaattatatc atcccgggcg tatcattacc agttttcttt ttcttttaca
     3121 aaaagatttg gaccaaatat cttttaggat atttgcccca aatccaattt ttttactttt
     3181 ccgatgctgt atccaatgcc tcgcttcttt cagctaatgc acgttcgatc gttcgatccg
     3241 ctaaaataga ttgagcatcg atcactgggt aatgattatc ttcagcaaat tcatgcatca
     3301 aagataattc tgtgcaacct aggatgactt tttcacagtt taggcgctct accgcttcct
     3361 ctaagatttc gtaatagagc tcttgattca aataatccga ttccttgatc tcatgataga
     3421 tcaggtaatt gattttttct tgaagcgcag tatcaggaat cactgtttcg aaaccaagat
     3481 ttttgacttc gcgttcatag attccagctt tcatacttcc ttctgttcct agaatcgcta
     3541 ctctccctgt cgtatgctga cggacaagtt catttgctgc ttctcttggc atatgcaaga
     3601 taggaatatc cgttgccgct tgtaactctt caaaaaagta gtgtgcggtg ttacaagtca
     3661 acacaatgaa gtttggtcgc aataaatttt gtttctcaat atcatccagc agaaacggca
     3721 ttggattttc ttctgatcga tctaaaatat aagctgtacg atcaggaact gttgcatgat
     3781 tgaataagac ataattcaaa tattcttgat cttttgtagc tttcgttcgg tggttgatca
     3841 agcgaacaaa actttctgtc gccatcgtgc ccattccgcc taaaatactg aaaaaattct
     3901 ccatgaacta gccttctttc acatgaaaat actttttgaa tcttggaata tagttatgga
     3961 acatatattt catcaacaac cagcgcttga ttgatcggtc tttttcataa aatacagtag
     4021 taccgtaacg attttctttg atcatttgct cagctagttt tttgtcttca ttttcccgag
     4081 catattctaa aaagattttt ttgggcacac ccatccataa tcttgcttta tctgattgat
     4141 tcgttccata ggtcgtttca acaaagggtt tgttgaacac tcgatcttct gtgacaaaac
     4201 gagccagatt caagccgttc aatgtcacaa agaaactgct acgaccttgt ctcaaattga
     4261 tttcaaataa tttatattct ccatctctag gatcatactt catatcgaaa ttggcaaagc
     4321 ctgtatattc gattttttcc aaaaatgcct taatggtttg atagattttt tcgttataat
     4381 ctggcatgat cacaacgtaa ttaccaatcg atgctggtgt aggatcttct aatagtggat
     4441 gaccaaggca catcatccga acttgatgat cttcatctac atacgcattt agcacacgca
     4501 tattactatc atcacccggg ataaaatctt gtacaatcat ttcactttta taaccggctt
     4561 cgtatatccg gcctaaaatt aaatcgaact cttctctagt ctctaaaata aaagcttttt
     4621 ttcttccttc aaattggaca gaaagatact ccacactgtt tgcaggtttc aaagcaactg
     4681 ggaattcaaa aggcaattcc tgttccaaat gtccatttac taacatttct tctctgacga
     4741 tcaatgtttt aggatacgga agatcatatt cttcacaaac ttcataaaag cttactttat
     4801 tgatcagcct ttcaaataaa gaataatcaa tatatggaca gataaaatat tctgacagct
     4861 cttgtttatg ttgggagatc aattctgcat atccatcacc acaagcaatc aaaagatatt
     4921 ttttactctt atcagaatat ctttcttttg ccaaacgaag catcgtctcg ataaatacag
     4981 ggtctttatc aaatcctgga ataacttcta cattgacaat tttactgtag cgggtagggg
     5041 ctaactgatc cgaagcatat gcttggcaga ttttgccgta tgcttcatta aaagaacgtg
     5101 ccataccata aacgttcatg tcgcttccta ataatatagg gataaattct tcattttcaa
     5161 tactgttcat cattatctct cacttactca taatctcttg tttttctttt ccaactacac
     5221 aaggaatatc ataccataaa gaaaaaagca atcaaaggaa agtcctccga ttgctcaatt
     5281 cttttttgaa cggattgaac ttataggaag tttttatcta taccaatcag tgtagaaaga
     5341 actaccatgt aatgattcta gaacactgat attaataaaa caagcaaaaa ttcatttttt
     5401 tatgtttttt taactatctc agctatctat cctctttata ccattatttt tttcaaatag
     5461 tgacctgtat aactttcttt tacgttaatg atttcttctg gtgttccagt cgcaaggatc
     5521 gttcctccac cttctccacc ttctggtcct aaatcaatga tataatctgc tgttttgatt
     5581 acatctagat tgtgttcaat cactaaaact gtgttaccag catctactaa tctttgtaat
     5641 acatgcaaca agcgggcgat gtcatcgcta tgaagtcctg tcgttggttc atctagtata
     5701 tagaaattct ttccattaga gattttgtgc aattcactgg caagtttcat ccgttgtgcc
     5761 tcaccaccgg acaatgtcgt tgctggttgc cccatcgtca cataacctaa gccaacatca
     5821 acaatcgttt gcagtttgcg atgaatcttt ggaatgtgct tgaagaattc tacagcatcc
     5881 tctactgtca tttccaaaat atcagcaatg ctttttcctt tgtaatgcac ttctaatgtt
     5941 tcagaattat atcgtttacc atgacatact tcacaaggaa catagacatc aggcaaaaag
     6001 tgcatttcga tcttaataat tccatccccg cgacaagctt cacaacgacc gccttttacg
     6061 ttgaaactaa aacgcccttt cttataaccc cgcatttttg cctcgttcgt ttgagcaaat
     6121 aacccacgga tatcatcaaa tacacttgta taagtcgctg gattacttct cggcgtccgg
     6181 ccgattgggc tttgatcgat atcgatgatc ttttcgatac tttcgtagcc ggaaattgtc
     6241 ttgaatttac ctggcttagc agaattctta tttaattttt gcgctaacga tttctttaag
     6301 atactattga ccaatgtact ctttccagaa ccagacacac ctgtgactgc tacaaattct
     6361 cctaatggaa attcaacact aatatttttc agattgtttt ctgaagcacc agtgatcttg
     6421 attgctttcc cattaccttt acggcgttct tttggtacag ggattgattt tttccctgaa
     6481 agatatttcc ctgtcaaaga attttcatca ttagctactt cttcaggagt gcctgcagcc
     6541 acgatttgtc cgccttgttc tccggcccca ggtccgacgt caatcaaata atctgctgcc
     6601 cacatcgtat cttcatcatg ttcgactaca acgagggtgt tgccaagatc acgcattttc
     6661 tttaatgagc caatcagtcg atcgttgtcg cgttgatgca atccgatcga tggttcatct
     6721 aaaatataca aaactccaga aagattcgat ccaatttgtg tagccaatct gattcgctgc
     6781 gcttctcccc cagataatgt tccggaagcg cgactcaaag ttagatagtc tagtcccaca
     6841 ttttccaaga atgaaagtct gtcatttact tcttttaaaa tcggtttggc aattactttc
     6901 tcttgttcag ataacgttaa gtttgagaag aaatctactg ctttttgaat agacaactca
     6961 tttgtttgcc cgatattcgt tccattgatt tgaacagata gagcttgggg attcaatcga
     7021 taacctttac atgtctgaca agttaattct gtcatataaa gacgcatttg ctcacgcgtg
     7081 aaatcactgt tcgtatcatg ataacgacgc tcgatattct tcaatacccc ttcgaaagtc
     7141 gtttctacat cgcgaacgcc accaaaatca ttttcataat ggaaatggaa aagatcatct
     7201 gtcccattca agatgatctc ctgatgctct tccggtaatt cttcgaatgg tgtatctaaa
     7261 tcgataccga aactttcaca agcctgcgct aacatttgag gataatactg agagctgatt
     7321 ggattccatg gaacgatagc accttgacta agtgtcttcg ttctatctgg aatcactaaa
     7381 tcttgatcga cttctaattt gacacctaaa ccatcacagt ctggacaagc tccaaaaggc
     7441 gcattgaatg agaacagtct tggttctagt tcgccaacag taaatccgca ataaggacag
     7501 gcatagtgtt cgctaaacaa catttcttct tcaccgatca cgtcaaccag agcatagccg
     7561 tctgctaatc gcagggccgc ctctaaagaa tcaaataggc gagaacgaat cccttctttc
     7621 acaatgatcc ggtcgatgac gatcgcgata tcatgttttt tgtttttctc cagctcaggc
     7681 acttcactta aatcatagat ctcaccatcg actcgaacgc ggacgtatcc ctctttttgg
     7741 attcgttcga atattttttt atgttgtcct tttttcttta ccacgatcgg tgccaacact
     7801 tggatctttg tgcgctcagg caaagccatt acttgatctg ccatttgatc tactgattga
     7861 ctggtaatct cgatatgatc gttgggacaa atcggatgtc ctacacgtgc aaacaacaag
     7921 cgtaaatagt cattgatttc cgtcacagta cctactgtcg accgaggatt tttacttgtt
     7981 gttttctgat caatagaaat cgccggactc aatccgtcga tgctatctac gtccggctta
     8041 tccatttgtc ctaaaaactg acgagcataa gcagaaaggc tctctacgta tctccgctgc
     8101 ccttcagcgt acagcgtatc aaatgctagg gaactttttc cagaaccgga caaacctgta
     8161 acgaccacga atttatctcg tggaatcgtt acatctatat ttttcaagtt atgggcacgt
     8221 gcaccatgaa tcgtaatttt atcgttagcc atgttgtcct cctactttga agcttttaat
     8281 tctaaaatca tatctcgcac attcgcggct gtttcaaaat ccaaagcttt tgccgcatct
     8341 ttcatttctt tttctaattt catcaataac gtgtcttttt cttccttcgt catttcttcg
     8401 tacgagactt gtaccgcttc ttttgtatca tcttccgatt ctttcgtaat agaaatcaaa
     8461 tctcggattt ctttaatgat cgtttttgga acgatcccat gttcttcatt gtacttctgc
     8521 tgaatcgtac gacgtcttga cgtttcatcc atcgctagac gcatagaatc tgttactttg
     8581 tctgcgtaca tgattacttt accttcttca ttccgagcag ctcgtccaat cgtttggact
     8641 aaggaacgtt cactacgtaa aaagccttct ttatccgcat ccaagatagc aacaagggaa
     8701 acttcaggta cgtctaatcc ttcacgaagc aaattgattc caactaacac atcaaattca
     8761 cctaatcgaa gatctcgaat gatttctgtt cgttccagcg ttttgatatc actatgcaaa
     8821 tatttgactt tgatccctaa ttctttgaaa taatctgtga ggtcttctgc catttttttc
     8881 gtcaatgtcg ttacaaacac ccgctggtct ttttcgactc gttcatttat ttcgcctact
     8941 agatcatcga tttgtcccat gattggacga acttcgatca ctgggtcaag cagccctgtc
     9001 ggacggatga tttgttggat aacggtatct gtttgttcat gttcatatgg tcctggcgtc
     9061 gcagagacat aaatgatctg attgacatgt ttttcaaact cttctaaccg aagtggccga
     9121 ttgtctaaag cagatggcaa acggaaaccg taatctacta gcatctgttt tctcgctcgg
     9181 tcaccattat acattcctct gatttgaggc atggttacgt gcgattcgtc cgccacgatc
     9241 aaaaaatctt caggaaagaa atccaatagc gtataaggcg gttcaccttc tttacgtccg
     9301 tccatatgcc gagaataatt ttcaattccc gacgtgtagc ccatttctaa catcatttca
     9361 atatcgtaat tggttcgctg ttctaaacgt tgagcttcca gaagtttgtt ttcatttcgc
     9421 aagacagtta aacgctgttc taattcctct ttgatattcg ctacggcatg ctccatatgt
     9481 tcatcattcg tcacaaagtg agtcgccggg aagattgcta cgtgttccgt ttctcctaaa
     9541 acttcacctg tcagtgcatt gacttctcgg atacgttcga cctcatctcc aaaaaactcc
     9601 acacgtaagg cacgttcatc tcttgaagcc ggaaaaatct ccacgacatc accgcgcact
     9661 cgaaaacgac cgcgctgaaa atctatatca tttctttcga attggatact gactaagtca
     9721 cgaatcaact gattgcgatc tagttctgct ccctgtcgaa tcgaaactac ttgtttttgg
     9781 tactcaaaag gcgagcctaa tccaaaaata catgaaacgg atgcaatgac gatcacatca
     9841 tttctttcta ataatgaact agtagccgaa tgtcgtaatt tgtcgatctc atcatttaca
     9901 cttgaatctt tttcgatata cgtatcgctt gaaggcacat aagcttcagg ctgataataa
     9961 tcataataag aaacaaaata ttcaacagca ttgttaggga aaaattcctt gaattccccg
    10021 tacagctgac cagccaatgt tttgttatgg gcaataatca gtgttggttt attcactttc
    10081 tcaataagat tggaaatcgt atatgttttc cctgttccag tcgcacctaa taagatttgt
    10141 gcttttttcc cgccgactac gccatccact aattggttaa tcgcttcagg ctgatcacca
    10201 gccggttggt atttcgagac aagctcaaag tgtcgggagg tatctctctc aatcattctt
    10261 cgtcctcctt ttcattcatt ccttttcatt ttacgaccaa taaaaagaaa aggaaagcaa
    10321 aggcattcct tactaaaaaa ttataccaca ccgaacatac tttcgccact tttttatctt
    10381 attatacatg ttatgtttta taacataaaa tgttagaaac tcgcattttt ttcatttttt
    10441 cgagcttaca gtcttgtctg attcagcact tttcgcctat aataataaaa tattggaaat
    10501 ttaggaggtt gattatgaaa agaaagcatt tatttcactc atttgttttc ttaatgacag
    10561 gacttgtgac acttgcgttt ggtcatgttg cacaagctga caacactaca acggcagagt
    10621 caagcacaag cgagacaatg caaaaaatag agcccaaaaa agacgtatac gtaattgcca
    10681 gtgactctgc ttttgctcca tttgaatttc aaaacgcaaa gggcgagtac gaaggcatcg
    10741 atgtaaaatt gatggaaaac atcgccaaac tgcaaggatt taacatccga atggactacc
    10801 gtggtttctc tgctgctttg caagctttgg aatcaggaca agcagacgga atgattgccg
    10861 gaatgactgt aacagatgaa cgaaagaaaa gctatgattt ttctgattct tactttgata
    10921 gcgggatcca attagctgtc aaaaaaggag atacttcgat caagtcttat gaagatttga
    10981 aaggaaaaac agtcggagca aaagttggta ccgaaagtgc tgaattttta agcgaccatg
    11041 aatctgaata tggctattct atcaaatatt ttgatgctgc tgatcaattg tacgacgggt
    11101 taaaagttgg cgcaatcgat gcaatgatgg atgattatcc ggtaatcggt tatgcgatca
    11161 aacaaggaca agaattggaa acaccgatca aaaaagaatc tggcggaaaa tatgcatttg
    11221 ccgttaaaaa aggacaaaat cctgagcttc ttaatatgtt caatgaagga ttgaaagaat
    11281 taaaaagaac aggcgaatac gataagatta ttaaagaata tgtagcggat ggttcagaaa
    11341 cttccagtga gtcttccact gctgatgaat caacatttct tggacttatc caaaacaact
    11401 atcaagcatt gttgaaagga ctttggagaa caattctcct aactttgatc tcctttgctt
    11461 tggctatagt aatcggtgtg atttttggat tattcagtgt agcaccaata cgcgctcttc
    11521 gcacaattgc tagtatttat gtcgatatca tccgaggaat tccaatgatg gttcttgcgt
    11581 tctttatttt ctttgggttg cctggtatcg ttggatttac gattcctgac ttccttgctg
    11641 gtgttatcac cttgactctt aacgcaagtg cttatatcgc agagattgtt cgaggcggga
    11701 tcaacgccgt tcctgtcgga caaatggaag cttccagaag tttaggtctt tcttataatc
    11761 ggacaatgca aaaaatcatt ttgccgcaag cgatacgcat catgatccca tcattcgtca
    11821 atcagttcgt tatctcatta aaagacacaa cgatcatttc cgcaatcggt gtagtcgaat
    11881 tgctgcaaac tggtaagatc attgttgcac gtacaacaca aagtacgtat gtttacttga
    11941 tcattgcgat catgtacttg attttgatca cagcattgac aaaattagca aaagttttag
    12001 aaaagaaggt gaagtaagat ggcagaaaaa atcttagttg agcatttagt caaaaaatac
    12061 ggcgataaca cagtattaaa cgacatcaat gtatccatca acgaaggaga tgtcgtctgt
    12121 atcattggcc cttcaggttc aggaaaaagt acattcttgc gttgcttgaa tcgtttagaa
    12181 gaaccttctg acggtgaaat tattattgac ggcgcccact tgatggataa aagcacagat
    12241 atcaacttgg tgagacaaca tatcggaatg gtattccaac actttaacct attccctcat
    12301 ctttctattt tggaaaacat cattttagca ccgatggacc tgaagaaaga atctagagaa
    12361 gaagcagaga aaaaagcaat caaattactg cagacagttg gtctagaaga taaaaaagat
    12421 atgtatcctg aaatgctttc tggtggacaa aaacagcgtg tggctattgc tcgtgctcta
    12481 gcgatgaacc ctgacatcat gctttttgat gagcctactt ctgcactcga tcctgaaatg
    12541 gtcggtgatg tcttgaacgt catgaaaaag ttagcagaac aaggtatgac gatggttatc
    12601 gttacgcacg aaatggggtt cgctaaagaa gtggccaatc gcgtcatgtt cattgacgac
    12661 ggaaactttt tagaagatgg aacgcctcaa caaatctttg aaaatccgca aaatgaacgg
    12721 acaaaagatt tcttgaataa agtattaaat atttaaaata aattatatat gtctttttga
    12781 ctatcttttt atatcttctt aaatgtccgc aaagccttat tttataggct ctacgggcat
    12841 tttgcttttg tggtaaacct cacatatcta ggtctatctt cttatatttt cactatcaag
    12901 cgtggttaaa atcgtggtaa atacttctat gcaattagac gctgaacctc tgattttgcg
    12961 gtatctatgg acgcatgagc ataccagttc attgtgatac tgatatttga atgccccatg
    13021 atatactgta aatcttttgg gttcatgttc ttgcttgcca gtcttgtgca gaatgtatgg
    13081 cgtagcgtat gcggtgtgat atgtggcaat ggattgtcct tgtggtgctt gttgtatttc
    13141 tttaccatac ggataaataa ggcgttataa tcaatcgcaa ctttgggctt gcccttatga
    13201 ttgacaaata ggaagttgcc ccgtccgtct atcacaaatg gttctgcctt tggacgtttt
    13261 ttcataacct gttgaaatgc ctgtatcgtt tctttgctta atggtacttg ccttattccg
    13321 ctttttgttt taggcgtttc aatataatag ccctgttcct tgctctttag taactggtgg
    13381 tcgataatca caacttcatt cttgaaatca atgtcggcta ctgtcagtcc gcacagttcc
    13441 gaaatacgaa gtcctgtctt taacagtatc agcacatcat cataatgctt gtgatacaca
    13501 ttgtccgtct tgatgaatga gagtaaggct tgttcctgtt cctctgtcaa tgcgactttc
    13561 tttttggtat cattttctag gacttcactc aacttgaaat caaagggatt tttcctcaca
    13621 taatcatctt gaatggctat gtagaatgac gcttttaacg agcgtttatg gttgttaatg
    13681 gtattgtagg aaaagccttt gtctttcatg cgtaacgccc attccttagc gtcagagggt
    13741 ttgattgtat caatgctcct agcacctaac ttgtcctctt tcaataaccg catgagttgt
    13801 tcccgttgtt tctgtgtgct tttcttcaca tttgccctct gtgcgttctg tttggcataa
    13861 agttggcaaa gcgtcatttt cttgccagtg ctgtcgatac cgtcctcaat atcccgtctt
    13921 atctgctgtt ccagttctcg aagtgagggt ttttcccgtt ttccctttgg tgtcgggtct
    13981 gtgggtgtca atctccaagc atacacatat tttgtgtttc caaatgcgtc tacatattta
    14041 tataagtatt ttctgtctgt tcgttggctc tctccagtat gcaggatacg acctttgtta
    14101 tcccgtcttt tttctttcat ggtgtctgct cctttccttg ttggaaagag ccttgatatg
    14161 acttgtgttc atcataacac atacaaggct catttgcatt agatagcgtc caatttatca
    14221 atgacctgtt caaactgtcg gcgtttaatc tgtatgcggt tgccattcat aatgagccag
    14281 ccagcgtcct tgttttcctc tgccaatcgt cttaacttgt tttctccgat acggaaatac
    14341 tttgacgctt cttcaatggt aagggtgtat ttttcccata cgggaatatc ctgtgcatta
    14401 ctcataaaac accccctcac tttcgattac gtctgctatg tccgtacaca tctgttccac
    14461 aagcatttcc tgcttttcag agaggtgggt acattcatca atcattttca tgtagtttga
    14521 gcctgtcagc ctgtcgattt cctctatcat cacgattgac ggcatgacct gtctttccag
    14581 ccattgcaga gtgcgttcca tattgacagg tacggggttc atttcaaaag gtaaactgtc
    14641 atgggaaatg aatatctccc acagcggata gtcgggaaat tgcacaaaat cagtaataag
    14701 gtaaaacacc agcccatgag ggttatatgt caataacaat tcctcaactg cctgtactgc
    14761 ttttgtacca cgcagacgaa tttcaaaacg attgataata tccgtatgtt tctttttggt
    14821 tgcctgttcc ttttgttttt catacaggca gaagtatttt gtgcttgcct ttgaaccgat
    14881 ataaagggtg cttgctaaat tacggttttt tccgcttagt tttccaccct gcacattttc
    14941 ataattttta cagcgacaat ccgcaccgcc atttctgtat ttttcagaca aaacgggaat
    15001 atccaacaga ccacacatat cattgattgc caagtcaaaa cgtcggataa caccgtcaca
    15061 gtcaagacag cggtttaaaa atgaatacca atctctgttc tgtgcctgca agacatattc
    15121 catgttgcgt gagcctgccc cttttaattc caagaatacg cccatgtgtt cgtcatcaga
    15181 agccatgacc ttgatttctc cgtaggcgta atgctcttta tagccataat acccatagtc
    15241 ataatggata aaataatctg ctttcatgcc aagcaccttt cggataattt ccaatgcgtc
    15301 agtcgttgga taacggacag acagataatc aacgagcaga taaaaaggct tatcgcagat
    15361 ataataattc agacaacgca ggataatatt ttgtgtttcc ttgctggcgt tggcttttcc
    15421 gttctcaaag cggttgatac tatatcttga tacatgggta agacttgcaa gttctccttg
    15481 tgaaagaccg cttgcaagac gtttgttctt catttcaagt gcaaattcta aattgttcat
    15541 tgtattcctc caatcccaca atagccttga aatacctgta aacctctatt caaaaagtgt
    15601 gatatgcact atcttcttaa aacttctcat tactactcac aactttggga ttgtatggtt
    15661 tttgacttgt cttttgtgaa atagtgcccc cctgttagat aacgggggca cagacaaggc
    15721 tcgcaggctc gccagccgac aaagcatttc ttccccttga cacaaggtgt catggcggta
    15781 agcctgagag gcttccgcca ccgtgtcaga aatactttgt cagcaagttc tgttttgcct
    15841 tttctaacaa gttccatttc aaccgatatt catatctacg gatttaatca gataggtatt
    15901 tctttttgaa tgtttttata tttcattcta ttgtgcttat tcgattacaa gaacatagta
    15961 attcttatat gcttatttgt caatatcttt ttttgccttt acaaatcata ttaagcatga
    16021 tagacttata atagcgaaga tataacgaga tatggagggt tatgcaatga aaataagcca
    16081 tttaggaaat aatatacaga ccataagaaa atttagggga atgaaacaac aggaacttgc
    16141 ggacaaaatc ggtatcaata tgcagagcct ttccaagatt gaaagagggt tgaactatcc
    16201 tgcctatgaa acgctggaaa agataatgga agtgctggac gttacgccca atgaattgtt
    16261 gtcgggagaa tggaagtatg tcaatcaatc tgaaaaggaa gtctgtcagt ttttaagagc
    16321 cgaagaacgt ctaaatgcag aactgaaaca tggacactat gataacttct ttgacagcga
    16381 ggaagaatgg ctggaatatg agttagaaaa gttacgggaa tatattacag actatatcaa
    16441 cgggaaaagc attgaagcgt ccgaccttta cccaatcaaa gaatttatcc agcatttgaa
    16501 gtttcaaaaa ttattagacc gctatgatga tttatacagt atggatatgt ttggggaaag
    16561 catagaggga cataagtata agacacctta tcaagtcgtg aaaatgataa acccgaactc
    16621 aaaagaagat atggaactgt tacgagaagt attaaggaat aacaattttg atgatgagga
    16681 cgagtaactt ttttcctctt gcgagaagat acagcaaaca aaaagcccga tcaagagtgc
    16741 aaagcaccac caatggtggc aaagctcttg acagggcttt ttcttttctg ttgtgggcaa
    16801 tcaagaggaa gaaagggata aaatctgatt tgtggtaaat caggcaaagg caacgcaggt
    16861 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
    16921 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
    16981 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
    17041 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
    17101 ttggatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc
    17161 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
    17221 aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa
    17281 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa
    17341 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
    17401 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
    17461 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
    17521 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
    17581 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
    17641 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
    17701 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
    17761 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
    17821 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
    17881 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgttct ctgttccctc
    17941 tttatcatgg aagtgcaaaa agtaatatag ggattgataa ccttatagaa gttattacta
    18001 ataaatttta ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa
    18061 ttgaatatac aaaaaaaaga caacgtcttg catatatacg cctttatagt ggagtactac
    18121 atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca gaaatgtata
    18181 cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa attgttattt
    18241 tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta ttgccacaga
    18301 gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg agtaaacctg
    18361 aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat ccgcttctac
    18421 gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
    18481 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
    18541 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
    18601 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
    18661 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatcg tttcaaaatg
    18721 cagttatgga ggggatacgc tatggctgtg aacaaggatt gtatggttgg aatgtgacgg
    18781 actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
    18841 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
    18901 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
    18961 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
    19021 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
    19081 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
    19141 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
    19201 ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat
    19261 gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggagatagt tattataaac
    19321 tacaagtgga tattgtgtcc tgtatgtgga aataaaacac gattaaagat aagggaagat
    19381 actgaattaa aaaaattccc cctctattgt ccgaaatgca gacaagaaaa tttaattgaa
    19441 ataaagcagt tcaaagtaac tgtgattaca gagccagacg caaagacgca gagccgataa
    19501 aatgagatta atacaatctc attttatcgg ctctttccgt tatgtatgga ttcttttaat
    19561 tagtcttcga tgtttcttgc ttcgttgata ccgctggcta aagattccat taaggatagt
    19621 tctttgtctg taaagctatc catgtatttc tctatctgta atcgtcgggt gctttttacc
    19681 aagttattag caggtaagaa aaattcatca acggaaacat gaagtaacga tacaaggtca
    19741 taaagaactt gtatgctggg gtgttgccct ttattttcaa tattagttaa gtaccgtggg
    19801 tcaatttcaa tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa tcgagcttct
    19861 ttaatggcta aaccaaaggc tctaaaatca tatttatctt cttttttacg catagtagac
    19921 cacctctata cattttattg ttcctactga attaaaaaca ggtatagaaa aacgtgttat
    19981 atggtttata ggtttatatt taataaaaag cactactaaa cgccaataaa aaaaaccgtt
    20041 atatggtagt gctatttacg ctgttaaaat attgtatatt acttccaaat ggcggtttgt
    20101 tggaggtcaa cgtcgccatg aagtacatca tatacaataa atttccttac attgggttct
    20161 tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc caccaatgtg gttttataaa
    20221 tcatatagat agaataacag aagcatgtaa acagagaaat aaatctgttt atatgctttt
    20281 ttggctattc agaacttttt tacaaagttt atttatcagt aatgcaacaa atcccccttt
    20341 cacattggga ctaagagtga aaggagataa acgagcaagg ctcacttcct ttcctagaca
    20401 gaaagggggt gagattatga ggacatcttc tttcgagcat atcgtcagat tacagttcaa
    20461 ttctttaatg ctgattgtca ttaagaacaa agtgaaaagt cgaaacagac agcttgcaag
    20521 acattctaaa cgtgaagtat tgttttgtga aatagccgaa acgaaacaag ttgaacgtgg
    20581 aacgattgat acctgttttt atgattgtgt atcatttaaa gtcttgcact ttacaattta
    20641 tgtatctgat gaaacacttg gtacagtttt acatagacta tcagaaaaac agcgtagtgc
    20701 gattttatta cgttattttc aaggaatgaa cgacagagaa atttcagaac tgtaccatgt
    20761 atcacgttct gctatttgca gtagaagaac tagaggacta aggaagttaa aaatgctttt
    20821 gagcgaaagg aagtaaagat tatggctatc cctcttttga attgatttgc aaagcgtcaa
    20881 gcggagatga aatggcagtt aaagagattt tgaagtttta tgacggttat atttctaaat
    20941 tatgcttgcg tcctttttac cactctgaaa gtggtaaaat cattatgcag gttgatgaag
    21001 aactaaaagg agaaattcat acagacatga tgaaagcaat attgaaattt gaaatcggag
    21061 tgaagtaatc aactgtataa aaaaatcggg agatacacag tttcgtgtta tctcccttag
    21121 tagttttacg gtcatatcaa ggctcattca atcgtggtaa attcgtggta agatgagtgt
    21181 ttttctatac ttcaaaatgc ttgaatgtat agtgtttatg cggataattg aaaattagat
    21241 ataaaattta ataaaaacca aaaatgcacg gtcgccatga agggaccgtg tatttttatc
    21301 ttcttatttt ctcacctaat cgtatcagcc gcatcgcttg ttttcctgca caaacaagca
    21361 atcgctctac ctgttccatc gcataagaaa gtaccatcgg ttcattgatg atatcaagta
    21421 catagtcgat accaccctca cttaattgag cttgattttt cttctccata ttatgtagtt
    21481 gtgggacaac tttctgacaa tcagcaattg tgtcaagaac ggatcaatcc tttattggag
    21541 ttgctcgaaa gggtcttctc ttattacgga caggctttgc tcactgtaca tagacaacaa
    21601 atcattatcc tagtcaatcg gatttccaga gcctcattgc tatcattgct tgataaaatc
    21661 cagacaaagt ttaataaaat gtatcagtta cagctaaact ttggaattgg gtcgctttgt
    21721 tatacagagc aagagacacc gcaatctttc cttcatgcca aacaagtttg cgaatggata
    21781 gctttccatc aatctgtgaa cgagatccgc ttttttgaag atttagacct cgggatcgtt
    21841 ttaccagcaa tcccgtcaga tcaacgaaca ttatatgtaa aacgtatttt gaaaagcctg
    21901 acagaagaag aagtacattt gttcaagaag actttggcat gcttcagtaa aaataacgga
    21961 agcatcaaaa attgctcaga agaattattt attcataaaa acaccttgca atatcgattg
    22021 aacaagttcc attctctcac tggttatacc cctagaaact atgacgatta tcatattcta
    22081 aagctggctt ttttgttggt acaaacatga caactacaaa aaaaccaggc gaacatatga
    22141 ttgttcgtct ggtttttaat tattaatgac ttagcgtttt tactagaaaa actacttccg
    22201 ctttttcccg ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc
    22261 aactttgatg ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag
    22321 atttttcggt gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa
    22381 cttcggagcg ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat
    22441 cttcaatttc acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg
    22501 tacaaaactt aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact
    22561 ttcttctaca cggaagacca catacaaatc accaaatggt ccgccgtttt ctccagcttc
    22621 tccttgacca gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt
    22681 aactgaatgt gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat
    22741 ttctttccct gtcccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg
    22801 tgtttggcgt tctacattaa tcgtacctgc accatgacat ttatgacaag tagttggttg
    22861 tgtgcctggt tttgcaccgt tcccaccaca agtatgacag atttcttccc gattatactt
    22921 gatttctttt tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa
    22981 atcagcacct tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga
    23041 gtcgaagata tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa
    23101 tccgccacca gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc
    23161 atatgcagcg cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa
    23221 tttttcttcc gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg
    23281 ataagctttc tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata
    23341 atcacgtttt gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg
    23401 ttatcatatc atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa
    23461 aaagccaaag tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc
    23521 gccatcgact tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc
    23581 tgggttttct tgagcttgtt gttgtgccgc ttgttcgtat agtttaacag tcaagttttg
    23641 aacgatttcg ttcaatgcat cgcgtttagt tttcatttct tcgatgttgt ttgcttcaac
    23701 agcggctttc aattcatcgc gagctgtttc tgcttttttc acttcttctt cgtccacttt
    23761 gccttctaat tctttcaatg ttttgtccac agagaatagc aatgcatcta catcattacg
    23821 taggtcaact tcctctttac gagctttatc tgcttctgca tttgcttctg catcttttac
    23881 catacgttcg atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc
    23941 tttttgtgta cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa
    24001 tgttacttcg atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct
    24061 tcctagagtt ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac
    24121 tgctggttga ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt
    24181 attgcggtca atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg
    24241 tgtaacgtca agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc
    24301 agcacccata gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc
    24361 tttacgtact gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc
    24421 gtcgatttct gattgtgata aacctgcatc ttttagcgct tgacgtactg gtacttttgt
    24481 acgttctact aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa
    24541 atgcaatggt ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac
    24601 gccagataaa tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt
    24661 gtcttgagac aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat
    24721 tttgttatca aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa
    24781 gacaccgtct cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac
    24841 taagattttt tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg
    24901 ttcattgacg atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg
    24961 acgttgtgca tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc
    25021 taaatagtct tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg
    25081 tgtatatgat ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat
    25141 tgatgaaatc gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat
    25201 ttctccattt ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat
    25261 gatttttgct tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat
    25321 accgataatt ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat
    25381 ccgcacaatt attgtgcaac aatgaccatg gttggtctta acacgcgatc atgtaatttg
    25441 tagccttctt gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca
    25501 gtttgtactg cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca
    25561 attccttctt cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc
    25621 aaacctgccc cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt
    25681 gatggaagta attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca
    25741 ttttttcctc gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc
    25801 atttcttcta actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa
    25861 atacccgaat cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt
    25921 tctttatcta gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc
    25981 agctgtttga ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat
    26041 tcacattacg agtttgacaa gtcaagcgaa cggtagtaat cagcaagttt agctgctagt
    26101 tctcttcgat aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct
    26161 aacaaggcta ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc
    26221 atattctgca ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc
    26281 atcggagtga tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg
    26341 aactgatcaa gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa
    26401 accttatctt caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc
    26461 gtttggaaat atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc
    26521 atcagaggat ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga
    26581 cttcccatat attcgggaag agtgaacact tggctttcta cattccccat atctgttacg
    26641 acgatagcaa taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg
    26701 tcttttattt caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca
    26761 gcagattgct taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc
    26821 agcagcaaat catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga
    26881 tagcctaaag cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca
    26941 tcttctaatg ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac
    27001 atcaatgttt tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt
    27061 aaaatgtcgt tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt
    27121 ctaagtgcta atccacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact
    27181 ccatttttta gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt
    27241 tatatcaacg tttcaatagt cctttattgt atttttgaac tttttttatt taaattcaga
    27301 taaaaaacaa atattcttta aactattttt caaacaaaaa gcgctcaaac acattgttcc
    27361 ccaaaaaggt ccctcttggt gtcaaataaa cacggtcttc ttcattaaca agtaaccctt
    27421 cttgctctaa ttcccttagc acatcaccgt acaccgattc aattgttttt tgatagcgat
    27481 catagaaatg ttttttagta atccctgttt ttttcttagt cctaagaaca tttcttcttc
    27541 tatttgattt tttcgtgtca atttttcttc ttccaatacc gggcgttcat ttgctcgtaa
    27601 tgggcgtaaa tagtgttgga ttggtccttt gttgcggtaa cgaatatctc caagataacc
    27661 gctagcacca gctccaaacc caaaatagtg gtcattgttc cagtacatga ggttatgttg
    27721 agattcatgt ccggagagcc caaagttact tatttcatat tggtgacgtc catgcttttc
    27781 cattgcttca atcgtctctt caaacattct ggtttccgtt tcctgatcag gcagttcgag
    27841 tcttccttgt cgtacccagt tcatgaacat tgttttgttt tcgaggatca atgagtacaa
    27901 agaatagtga ggcagatcca aagccaaagc tttttccaaa gtatccagat atccttctag
    27961 tgtttgtccc ggtaatgcat aaatcaaatc gatgcttaca ttggtaaaat tttctgcttc
    28021 taaaaattgc atagtttgat acacatcttc tgcggtgtgt ttacgtccaa tttttttcaa
    28081 taagcgatta tcaaatgtct gcactcccat ggataaacga ttgacgccat agtttttcat
    28141 tacttgaagt ttttcacggg tcaaatcacc tggattcgct tctacagtaa actcattacc
    28201 attttgaaaa ggcaggatct ctctagcacc tgataacagt cgatcaagtt gtttagcaga
    28261 taaagaagtt ggtgtcccgc cccctacgta aagtgtttct aaattcgctg tcggatgact
    28321 ttctagcatt attttcattt cattcagcag catttccaca tactcatcta ctggctgccc
    28381 ttctaaaaaa actttattaa aatcacagta atagcagata tgctcgcaaa atgggatatg
    28441 aatgtacgca gaaaccttgt tttctgcggg atttattgtt tctatcattg aaattctttc
    28501 tccttacgtc gaaaagtcgt gcctgttttc gaccttctta aaatcgcgtt caagcaccaa
    28561 ctcattgaaa taagccgcat atttagaatc aaggaaacct agctctaaaa atgcttgctt
    28621 attgtcatcg gagctacacg gtgcttttac agcctctcga taaacggtaa tcgcaaagct
    28681 gaccttcggc attaagtcaa aatctgcaaa aatatgaaaa gctattttca cagattcctc
    28741 cttaagtgct cagaccacaa cgctttgctt acaacctctc atatacgcat actaaacgta
    28801 tttggcgaac aaatccacta aaaaatccaa gaatcgactt cttggatgat tttttctttc
    28861 attaaactgg ttacctagtt atatatctct agggttgttt tcatgtctga gtgcccgata
    28921 tgatttattt ttcgtttcta taattacttt ttgccttcca gtttctttta atattgtact
    28981 caagcaagtg ggaattggtt cttttgtcat ccatgtatgc aaaatgttat aatgctggta
    29041 aacaaaaatg attacctgtc atttttattt agttcgtatt taattaagtt gactatctgt
    29101 atactctgtc taaaacttaa gaattgttgt tttcgctgtc gctacccaat tttaatgaat
    29161 gtctgtaggt taaaactgtt gtccttgtta tagatagtaa aggagagctt gaaaatgaaa
    29221 actgcgatta cgtttctatt agaggaatat gccgattggg aaggtgctta tattactagc
    29281 acactaaacc aaaatgagaa atggcaaaac aaaactgctt ctatccaatc agaggtcact
    29341 tccattggag gattaaaaac aaaaatcgat tacttaatcg aacaaatacc tgaaaaagtt
    29401 gaccttttta ttatgatcgg tggcaattca tgggacataa aaaatcatca tttatatcaa
    29461 ataatcgata aattgttaaa aacagacgta ccagtagctg ctatttgtgg ggcagttgat
    29521 tatttagctc agagcggatt attgcaaggt tacaaacaca ccggaaatgc gcaatttcct
    29581 tggttagaat cgg
//
LOCUS       DAZKMZ010000026        29379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000026 DAZKMZ010000000
VERSION     DAZKMZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.026"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1108
                     /locus_tag="ACG8M5_001290"
     CDS             <1..1108
                     /locus_tag="ACG8M5_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0358556.1"
                     /translation="VETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLT
                     KIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPTGY
                     ELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGT
                     VLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTK
                     ENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGA
                     YQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1461..2309)
                     /locus_tag="ACG8M5_001291"
                     /pseudo
     CDS             complement(1461..2309)
                     /locus_tag="ACG8M5_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2462..3574
                     /locus_tag="ACG8M5_001292"
     CDS             2462..3574
                     /locus_tag="ACG8M5_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0358557.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3652..3945)
                     /locus_tag="ACG8M5_001293"
     CDS             complement(3652..3945)
                     /locus_tag="ACG8M5_001293"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0358558.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            4113..4427
                     /locus_tag="ACG8M5_001294"
     CDS             4113..4427
                     /locus_tag="ACG8M5_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0358559.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4448..6427
                     /locus_tag="ACG8M5_001295"
     CDS             4448..6427
                     /locus_tag="ACG8M5_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0358560.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6440..6766
                     /locus_tag="ACG8M5_001296"
     CDS             6440..6766
                     /locus_tag="ACG8M5_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0358561.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6782..7300
                     /locus_tag="ACG8M5_001297"
     CDS             6782..7300
                     /locus_tag="ACG8M5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358562.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7300..8652
                     /gene="celB"
                     /locus_tag="ACG8M5_001298"
     CDS             7300..8652
                     /gene="celB"
                     /locus_tag="ACG8M5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0358563.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8736..10241)
                     /locus_tag="ACG8M5_001299"
     CDS             complement(8736..10241)
                     /locus_tag="ACG8M5_001299"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0358564.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10333..10923)
                     /locus_tag="ACG8M5_001300"
     CDS             complement(10333..10923)
                     /locus_tag="ACG8M5_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0358565.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11102..12748
                     /locus_tag="ACG8M5_001301"
     CDS             11102..12748
                     /locus_tag="ACG8M5_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0358566.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12797..13516
                     /locus_tag="ACG8M5_001302"
     CDS             12797..13516
                     /locus_tag="ACG8M5_001302"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0358567.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13995..14942
                     /locus_tag="ACG8M5_001303"
     CDS             13995..14942
                     /locus_tag="ACG8M5_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358568.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14954..15874
                     /locus_tag="ACG8M5_001304"
     CDS             14954..15874
                     /locus_tag="ACG8M5_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358569.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15915..17372
                     /locus_tag="ACG8M5_001305"
     CDS             15915..17372
                     /locus_tag="ACG8M5_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358570.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17717..18331
                     /locus_tag="ACG8M5_001306"
     CDS             17717..18331
                     /locus_tag="ACG8M5_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0358571.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18324..20048
                     /locus_tag="ACG8M5_001307"
     CDS             18324..20048
                     /locus_tag="ACG8M5_001307"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0358572.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            20045..21496
                     /locus_tag="ACG8M5_001308"
     CDS             20045..21496
                     /locus_tag="ACG8M5_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0358573.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21969..24122)
                     /locus_tag="ACG8M5_001309"
     CDS             complement(21969..24122)
                     /locus_tag="ACG8M5_001309"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0358574.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(24124..25170)
                     /locus_tag="ACG8M5_001310"
     CDS             complement(24124..25170)
                     /locus_tag="ACG8M5_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0358575.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25320..26609
                     /locus_tag="ACG8M5_001311"
     CDS             25320..26609
                     /locus_tag="ACG8M5_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0358576.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26622..29324
                     /locus_tag="ACG8M5_001312"
     CDS             26622..29324
                     /locus_tag="ACG8M5_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0358577.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9560 a         5084 c         5981 g         8754 t
ORIGIN      
        1 ggtagaaacc caagcgccaa tagggtatga attagatgcg acaccaatcg agtttgagat
       61 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta caccaggcgg
      121 tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaaggag ctgtttttga
      181 gttacagaac cgagaaggag aaacgctaca aaccggtcta actacaggag aagatggaaa
      241 actggcaatt gacggtttag caccaggagc atatcagctg gtagaaactc aagcgccaac
      301 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
      361 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      421 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      481 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      541 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      601 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      661 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      721 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      781 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      841 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      901 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      961 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
     1021 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
     1081 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
     1141 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
     1201 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
     1261 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1321 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1381 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1441 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1501 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1561 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1621 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1681 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1741 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1801 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1861 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1921 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1981 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     2041 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     2101 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     2161 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     2221 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     2281 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2341 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2401 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2461 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2521 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2581 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2641 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2701 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2761 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2821 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2881 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2941 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     3001 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     3061 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     3121 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     3181 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     3241 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3301 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3361 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3421 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3481 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3541 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3601 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3661 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3721 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3781 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3841 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3901 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3961 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     4021 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     4081 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     4141 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     4201 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     4261 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4321 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4381 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4441 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4501 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4561 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4621 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4681 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4741 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4801 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4861 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4921 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4981 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     5041 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     5101 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     5161 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     5221 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     5281 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5341 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5401 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5461 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5521 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5581 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5641 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5701 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5761 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5821 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5881 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5941 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     6001 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     6061 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     6121 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     6181 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     6241 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6301 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6361 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6421 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6481 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6541 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6601 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6661 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6721 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6781 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6841 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6901 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6961 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     7021 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     7081 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     7141 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     7201 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     7261 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7321 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7381 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7441 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7501 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7561 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7621 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7681 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7741 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7801 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7861 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7921 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7981 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     8041 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     8101 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     8161 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     8221 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     8281 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8341 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8401 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8461 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8521 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8581 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8641 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8701 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8761 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8821 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8881 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8941 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     9001 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     9061 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     9121 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     9181 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     9241 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9301 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9361 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9421 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9481 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9541 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9601 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9661 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9721 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9781 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9841 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9901 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9961 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
    10021 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
    10081 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
    10141 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
    10201 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
    10261 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10321 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10381 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10441 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10501 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10561 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10621 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10681 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10741 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10801 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10861 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10921 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10981 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    11041 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    11101 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    11161 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    11221 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    11281 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11341 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11401 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11461 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11521 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11581 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11641 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11701 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11761 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11821 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11881 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11941 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    12001 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    12061 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    12121 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    12181 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    12241 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12301 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12361 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12421 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12481 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12541 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12601 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12661 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12721 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12781 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12841 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12901 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12961 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    13021 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    13081 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    13141 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    13201 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    13261 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13321 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13381 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13441 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13501 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13561 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13621 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13681 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13741 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13801 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13861 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13921 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13981 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    14041 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    14101 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    14161 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    14221 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    14281 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14341 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14401 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14461 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14521 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14581 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14641 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14701 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14761 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14821 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14881 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14941 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    15001 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    15061 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    15121 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    15181 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    15241 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15301 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15361 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15421 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15481 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15541 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15601 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15661 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15721 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15781 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15841 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15901 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15961 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    16021 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    16081 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    16141 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    16201 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    16261 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16321 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16381 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16441 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16501 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16561 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16621 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16681 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16741 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16801 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16861 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16921 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16981 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    17041 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    17101 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    17161 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    17221 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    17281 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17341 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17401 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17461 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17521 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17581 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17641 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17701 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17761 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17821 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17881 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17941 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    18001 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    18061 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    18121 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    18181 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    18241 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18301 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18361 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18421 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18481 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18541 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18601 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18661 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18721 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18781 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18841 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18901 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18961 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    19021 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    19081 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    19141 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    19201 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    19261 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19321 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19381 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19441 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19501 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19561 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19621 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19681 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19741 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19801 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19861 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19921 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19981 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    20041 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    20101 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    20161 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    20221 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    20281 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20341 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20401 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20461 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20521 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20581 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20641 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20701 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20761 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20821 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20881 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20941 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    21001 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    21061 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    21121 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    21181 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    21241 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21301 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21361 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21421 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21481 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21541 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21601 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21661 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21721 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21781 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21841 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21901 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21961 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    22021 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    22081 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    22141 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    22201 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    22261 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22321 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22381 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22441 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22501 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22561 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22621 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22681 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22741 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22801 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22861 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22921 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22981 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    23041 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    23101 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    23161 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    23221 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    23281 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23341 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23401 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23461 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23521 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23581 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23641 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23701 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23761 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23821 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23881 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23941 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    24001 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    24061 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    24121 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    24181 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    24241 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24301 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24361 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24421 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24481 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24541 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24601 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24661 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24721 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24781 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24841 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24901 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24961 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    25021 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    25081 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    25141 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    25201 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    25261 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25321 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25381 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25441 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25501 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25561 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25621 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25681 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25741 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25801 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25861 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25921 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25981 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    26041 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    26101 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    26161 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    26221 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    26281 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26341 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26401 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26461 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26521 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26581 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26641 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26701 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26761 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26821 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26881 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26941 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    27001 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    27061 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    27121 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    27181 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    27241 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27301 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27361 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27421 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27481 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27541 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27601 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27661 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27721 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27781 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27841 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27901 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27961 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    28021 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    28081 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    28141 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    28201 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    28261 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28321 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28381 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28441 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28501 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28561 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28621 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28681 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28741 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28801 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28861 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28921 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28981 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    29041 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    29101 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    29161 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    29221 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    29281 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29341 atttgtgtgt gcggtttcag atggtggaga aattttaga
//
LOCUS       DAZKMZ010000027        28603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000027 DAZKMZ010000000
VERSION     DAZKMZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.027"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8M5_001313"
     CDS             237..1877
                     /locus_tag="ACG8M5_001313"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0358578.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8M5_001314"
     CDS             2031..3353
                     /locus_tag="ACG8M5_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0358579.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8M5_001315"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8M5_001315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358580.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8M5_001316"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8M5_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0358581.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8M5_001317"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8M5_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0358582.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M5_001318"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M5_001318"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0358583.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8M5_001319"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8M5_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0358584.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8M5_001320"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8M5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0358585.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8M5_001321"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8M5_001321"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8M5_001322"
     CDS             7490..8242
                     /locus_tag="ACG8M5_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0358586.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8M5_001323"
     CDS             8258..8737
                     /locus_tag="ACG8M5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0358587.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8M5_001324"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8M5_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0358588.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8M5_001325"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8M5_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358589.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8M5_001326"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8M5_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0358590.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8M5_001327"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8M5_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0358591.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8M5_001328"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8M5_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0358592.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8M5_001329"
     CDS             14472..14825
                     /locus_tag="ACG8M5_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0358593.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8M5_001330"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8M5_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0358594.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8M5_001331"
     CDS             15781..17715
                     /locus_tag="ACG8M5_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0358595.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8M5_001332"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8M5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0358596.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8M5_001333"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8M5_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0358597.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8M5_001334"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8M5_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0358598.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8M5_001335"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8M5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358599.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8M5_001336"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8M5_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358600.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8M5_001337"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8M5_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0358601.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACG8M5_001338"
     CDS             25388..26296
                     /locus_tag="ACG8M5_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0358602.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACG8M5_001339"
     CDS             26437..28050
                     /locus_tag="ACG8M5_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0358603.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAZKMZ010000028        28259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000028 DAZKMZ010000000
VERSION     DAZKMZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.028"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG8M5_001340"
     CDS             complement(27..923)
                     /locus_tag="ACG8M5_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0358604.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG8M5_001341"
     CDS             complement(916..1596)
                     /locus_tag="ACG8M5_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0358605.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG8M5_001342"
     CDS             complement(1597..2379)
                     /locus_tag="ACG8M5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0358606.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG8M5_001343"
     CDS             complement(2372..3289)
                     /locus_tag="ACG8M5_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0358607.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG8M5_001344"
     CDS             complement(3453..3707)
                     /locus_tag="ACG8M5_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358608.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="ACG8M5_001345"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="ACG8M5_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0358609.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="ACG8M5_001346"
     CDS             7904..8218
                     /locus_tag="ACG8M5_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0358610.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="ACG8M5_001347"
     CDS             8231..8605
                     /locus_tag="ACG8M5_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0358611.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="ACG8M5_001348"
     CDS             8615..8950
                     /locus_tag="ACG8M5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0358612.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="ACG8M5_001349"
     CDS             9033..10373
                     /locus_tag="ACG8M5_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0358613.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="ACG8M5_001350"
     CDS             10451..11125
                     /locus_tag="ACG8M5_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0358614.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11118..11282
                     /locus_tag="ACG8M5_001351"
     CDS             11118..11282
                     /locus_tag="ACG8M5_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358615.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11358..12500
                     /gene="dcm"
                     /locus_tag="ACG8M5_001352"
                     /pseudo
     CDS             11358..12500
                     /gene="dcm"
                     /locus_tag="ACG8M5_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12490..12780
                     /locus_tag="ACG8M5_001353"
     CDS             12490..12780
                     /locus_tag="ACG8M5_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358616.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /gene="mobT"
                     /locus_tag="ACG8M5_001354"
     CDS             13039..14223
                     /gene="mobT"
                     /locus_tag="ACG8M5_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0358617.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14220..14357
                     /locus_tag="ACG8M5_001355"
     CDS             14220..14357
                     /locus_tag="ACG8M5_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0358618.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG8M5_001356"
     CDS             15103..17013
                     /gene="ltrA"
                     /locus_tag="ACG8M5_001356"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0358619.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17117..17341
                     /locus_tag="ACG8M5_001357"
     CDS             17117..17341
                     /locus_tag="ACG8M5_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358620.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17354..17857
                     /locus_tag="ACG8M5_001358"
     CDS             17354..17857
                     /locus_tag="ACG8M5_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0358621.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17917..18306
                     /locus_tag="ACG8M5_001359"
     CDS             17917..18306
                     /locus_tag="ACG8M5_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0358622.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18293..20740
                     /locus_tag="ACG8M5_001360"
     CDS             18293..20740
                     /locus_tag="ACG8M5_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0358623.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20745..22868
                     /locus_tag="ACG8M5_001361"
     CDS             20745..22868
                     /locus_tag="ACG8M5_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0358624.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22865..23869
                     /locus_tag="ACG8M5_001362"
     CDS             22865..23869
                     /locus_tag="ACG8M5_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0358625.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23888..24799
                     /locus_tag="ACG8M5_001363"
     CDS             23888..24799
                     /locus_tag="ACG8M5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0358626.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            24986..25242
                     /locus_tag="ACG8M5_001364"
                     /pseudo
     CDS             24986..25242
                     /locus_tag="ACG8M5_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25459..25674)
                     /locus_tag="ACG8M5_001365"
     CDS             complement(25459..25674)
                     /locus_tag="ACG8M5_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358627.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(25923..26141)
                     /locus_tag="ACG8M5_001366"
                     /pseudo
     CDS             complement(25923..26141)
                     /locus_tag="ACG8M5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(26436..27875)
                     /locus_tag="ACG8M5_001367"
     CDS             complement(26436..27875)
                     /locus_tag="ACG8M5_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0358628.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9209 a         5064 c         5916 g         8070 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt tatgtgcgac tcgttcctta
    14401 gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg gttattagga gaactgataa
    14461 tctgacaggt ggaatgatga agtaacgctc tgaaacgcct gccctgagcc tccgacaagc
    14521 attgtatgag gaaactcaga atgtttgaag ctcggtaaag acggctgaaa gataccgtaa
    14581 tagttgatga acatatatct tctatcgaaa actatccaga ggtgggtagc gtatccaaca
    14641 ggtcgggggt ctataaaata tctatggtta gaatgttcta taaggaactg acgaaacagc
    14701 gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg
    14761 gtgtggcaaa gtaagaatat aggttatgaa atgccatata acgttacagg cggtatcaag
    14821 ccagcaggct tatagctagt gacctaagga tatatatgta tagataagat tatcggaacg
    14881 aggaaaggta ttgggttctc atcaagagaa taaactgacg aagaacaata agcagttgaa
    14941 ctaatgctga aaagcagagg gcgaacctaa gaaattcttg taatgagaat ggaggaatgc
    15001 cctcaagtcg tgtatatatt agaaaagtat tatgttgttt caataaggat tacgagtaag
    15061 ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat
    15121 gaaacattgt gtgtagaaga tttaagacac gctgaatatt acgaaatgca aaacaccttt
    15181 gatgatttat atgccaaaag taagaatgga gatattttca ctcatcttat ggatattatc
    15241 ctatcgagag aaaatatatt actggcatat cgaaatatca aagcaaatgc aggcagtaaa
    15301 acagcaggaa cagacggtac aataattaaa gatattggca aattgccagc agaaacagtt
    15361 gtcaaaaaag taagatacat tgttgcaggc agtccacatg ggtatcgacc taagccagtg
    15421 aggcgaaaag aaataccaaa accaaatggt aaaaccagac ctctaggtat tccttgtatg
    15481 tgggacaggc ttatacaaca atgtataaaa caagtattag aaccaatctg cgaagctaaa
    15541 tttagtgaaa acagctatgg ttttagacct aataggtcag ttgaaaatgc aataaaagca
    15601 acctataacc gcctacaaat cagccagcta cattatgtga ttgaatttga tattaagggt
    15661 ttctttgaca acgtgaacca ttccaagctg ataaaacaga tatgggctat gggaatacgg
    15721 gacaagcact tgatattcat tttaaaaagg atattaaaag ctccaatcaa aatgactaac
    15781 ggtacaataa catatccaga gaaaggaact ccgcaagggg gaataatatc cccactacta
    15841 gcaaacattg ttcttaatga gttagaccat tgggtagaaa gccaatggca ggaaaatcct
    15901 gttacaaaaa actatgtggt tcatatcaat aaaagcggta gcccatgtaa aagcaacgct
    15961 tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata tggttcgtta tgcagatgat
    16021 tttagagttt tctgccgcta caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa
    16081 tggattgaac aacgattaaa attggaagta tcacaagaaa aaacaagaat tgtaaatgtt
    16141 agaaaaagat actccgactt tcttggtttc aagattaaaa tgataccgag aagaaagaag
    16201 ttagttgtta aatcacatat aagtgataaa caattcaaaa accaaaaaga caagctggta
    16261 tcacaggcaa agaaaattgc ttctccctca aaagggaaaa cggaattaga agaaacaaga
    16321 ctttataatt caatggtatt aggtatgcaa aattattatc agattgcaac gaatgtaaat
    16381 ctggattgta tgaaactgaa tagagctgta atggctatct tcactaacag attaggtaca
    16441 aacaaaaggg gcagattgag aaaaacaggc agacaactta cgacaaagga ggcaacaaga
    16501 tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg gcgaacctat tttccctgtt
    16561 ggatatatcc aacacagaaa accaatggca aaggtgtatt tagcgaattg ctacactcta
    16621 gagggaagaa aatttatcca caccaatcta agtgttgata aattactgat gtaccagtta
    16681 atgaaattat ctttggaaaa tcgcacaata gaatacgcag acaatcgaat ttcgctattt
    16741 tctgctcaac atggaaaatg tgctataact ctggaagaat ttcaacaagc taatgaaata
    16801 cattgtcatc atattgtacc aactggagct ggtggaaatg acgattataa aaatcttatt
    16861 ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa
    16921 tatatagctc tattgaagct aaataaagtc cagctcataa gactaaataa atatagagta
    16981 ttagctggca atcaagaatt aaacttgata taggtctact acataaatac ttttcaatat
    17041 aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa
    17101 gtttgagata tttataatat caggaactta cctatccata tatcaatggt ttttaaccaa
    17161 tgcccaatcc ttagtcttac tggcaattgt ggtcattggt ttgtttttag gatttaagcg
    17221 ggagttttcc aaactgattg gctttttggt gattgctttg attgcggtcg gattggtctt
    17281 taatgcttct ggggtgaagg atgtcttact cagtttgttt aaccgaattg tcggtgcgta
    17341 agggggaagc acgatggaac agatgcgtgt ttatattgcg aacctcggga aatataacga
    17401 aggtgaattg gtgggggcgt ggtttacccc acctgtcgat tttgatgaag tgaaagaacg
    17461 aattgggtta aatgacgaat acgaggaata tgcaattcat gattatgaac ttccctttga
    17521 gattgatgag tatacgccaa ttgaggaaat taatcggttg tgtggcttag cggaggaact
    17581 tgaaggaacg ccagttggtg aagttgcttc tgagattcaa catgctttct tcaattcttt
    17641 tgaggagatg gtagagcatg tggatgatat tatctattat ccagattgcg atgatatgag
    17701 tgatgtagca tatttcttga ttgtagaagc tggtgatttt ggagaagtac cagaacactt
    17761 gaagacgtat attgattttg atgcgtgtgg tagagacttg gagattggag gcaattattt
    17821 ggtaacgagt cgaggtgttt ttgagtatcc atattaacaa aattattgaa gggtagttga
    17881 tttctaaact atccttcttt ttaaaggaat gatttgatga agaaaataaa aagctacacc
    17941 agtatttggg cagttgaaaa ggtcatttat gcaattaatg actttcaact gccttttcct
    18001 gtgactttta accagatggc ttggtttgtt ttatccttgc tgtttgtcat cgtatttgcc
    18061 catgtgcctc cactatctat gatagagggg gcctttttaa aatacttagg tataccggtg
    18121 gcagttactt ggttcatgtc gcaaaaaacg tttgacggta agaaaccatt gggattttta
    18181 cgatctttta ttagctatca cttacgattt aaggtcactt tcgcaggaaa gaaagtcaaa
    18241 gaacagaaga aacgatggga cgaacccatt actctggtaa ggagtgtgaa ctatgtaccc
    18301 gattaagtat attgaaaata atcttgtctt taatcaagag ggagaatgtt ttgcctacta
    18361 tgagttaata ccttacaatt actccttttt gtcacctgaa cagaaatatc aagtccacga
    18421 taactttcgt caattgattg cgcaaaatcg tgaaggcaag attcatgcct tacaaattgc
    18481 cacagaaagt agtattcgtg ccacgcaaga acgatcaaaa aaggaaatta ccgggcgatt
    18541 aacagaagta gcgaaacaac gaattgattt acagacagag gcattagtat ccatgattgg
    18601 cgatagtcag attgattatc gcttttttat tggcttcaaa ctgattgcga cagatgaaga
    18661 ggtcaatcta aagaatctca agaaatcttt cttctccggc tttcaagaat ttgtctatgg
    18721 agtgaatcat catttgatgg gcgattttgt ttccttatcc aatgaagaaa ttcgccgata
    18781 taccaagctt gaaaaactaa tggaaagtaa attggctcga agatttaaag tcagacgagt
    18841 cacaccgagt gatctgatgt atttgattga acatatctat ggcgaaaagg gtacaccatt
    18901 tgaagaatat gaatttcagc taccaaagaa aaagctgaag tcggaaactt tggtgaaacg
    18961 ttacgactta ttacgtccca gtcgttgttt gattgaagaa aagccccggt acttatgcat
    19021 ggaacatgag aatcatgaat catatgtagc gtacttaaca attaatacaa ttgtggggga
    19081 aatggagttt ccctcctcag aacttttcta ttaccagcaa caacaattta cattcccaat
    19141 tgacactagt atgaatgtgg agattgtgac gaacaaaaag gcacttgcta ccgttcgcaa
    19201 taagaaaaag gaattaaaag acttggacaa ccatgcctat caatctgaca atgaaacaaa
    19261 ttccaatgtc ttagatgcat tggattccgt agatgaattg gaaactacct tggatcagtc
    19321 caaagaatcc atgtacaaac tgagctatgt ggttcgagta agtgccgaat cagtagatga
    19381 actaaagcgc cggtgtgatg aagtgctaga tttttatgat gataccaatg taaaactagt
    19441 ccggcctttt ggcgatatga tggggctaca tgaagaattc ttaccttcta gtaagcgata
    19501 catgaatgat tatatccagt atgttacgtc tgattttctc gccggacttg gttttggcgc
    19561 cactcagatg ttaggggagt tggaaggcat ttactttggt tataacgtag atacggggcg
    19621 caatgtttac ctgaaaccgg cattggcctc acaaggggtc aaggattcag ttaccaatgc
    19681 gctagcagcg gcattcctcg gttcactcgg tggcggaaag tcctttagta acaatctctt
    19741 agtttattac gccgtccttt ttggtggaca agctgtgatt gtcgatccaa aaggggaacg
    19801 gggtggctgg aaagaaacct tgccagaaat tgcggatgaa atcaatattc tcaacttaac
    19861 tagtaagcca aaaaatcaag gattacttga tccttatgtg attatggaga aaaagaagga
    19921 ttctgaaagt ctagcaattg atattctgac ctttttaaca ggaatttcta gtcgagatgg
    19981 ggagaagttt ccagtgttgc gtaaggccat tcggacggtt acccaaagca atcaacgggg
    20041 attattacaa gtaattaatg agcttcgaaa agaaggctca ccagttgcgg aaaatatcgc
    20101 ggatcacatc gagtccttta ccgattatga ttttgcccat ttgcttttct cagatggttc
    20161 gattacccag tcgattagtt tagataggca gttaaatatc attcaagtag cggatttggt
    20221 tttaccagat gcagaaacca cttttgaaga atataccacc atggaattgc ttagtgtttc
    20281 catgctaatc gtcatttcca cttttgcctt ggactttatc catagtaatc gagaaatctt
    20341 caaaattgta gacttagatg aggcgtggag ttttcttcaa gtagcgcaag ggaaaacatt
    20401 atcgaataag ctcgttcgtg ctgggcgggc tatgaatgcc ggggtttact ttgtcacgca
    20461 aaatagtgat gatttactgg atgaacgctt gaagaataat atcgggttga agtttgcgtt
    20521 tcgcagtacc gatattcatg aaatcaagaa aaccttggaa ttctttggct tggatcgaga
    20581 agatgaaagc aatcaaaaac gcctacgaga tttggaaaat ggccaatgct tgatgcagga
    20641 tttgtatggt cgggtaggcg tggttcaggt acatccagtc tttgaagaac tcttccatgc
    20701 ctttgatacc cgtccacctg ttcaagagag aatggagtga ggggatgaaa aagaaacttt
    20761 ttcggattgt gggaatcgtt tttattagca tactcactct gatagttcta ttgagtttag
    20821 ttggtacggt agccgaggcg acagggctag ttgatgatac agttgaagca ggaaatctat
    20881 attccaaata ttcactgaac aactatcaac tagatttctt tgtggatagc tcttgggatt
    20941 ggttgccttg gaattggggt gatggcttag ggaaaagcgt gatgtatggt ctttatgcca
    21001 ttactaattt catttggacg gtgagtttgt atttgtcgaa cgccacgggt tatgtggttc
    21061 aagaggctta caaattagat ttcatttcgg atacggctga aagtattgga aagaatatcc
    21121 aaacattggc tggtattact gaaaatggac tccagacctc cggattttat tttgggtttc
    21181 tattattgat gattttagct ctgggggtgt atgtggcgta taccggatta ttaaaacgag
    21241 aaaccacgaa agcagttcga gccgtgatta attttgtgat gatttttcta ctatcgggtt
    21301 cgttcattgc ttatgcgcct acctatatca caaaaatcaa tgactttagc tcagatgtta
    21361 gtgaagcggc ccttaccctt ggaaccgaga tcgttgtacc caattcagaa agccaaggaa
    21421 aagatagtgt ggatttgatt cgggatagtt tgttttcgat tcaagtccaa caaccttggt
    21481 tactactgca atttgatgat tccaacatag aagaaattgg tgaagaccgg gtcaacaaga
    21541 ttctatctgt gagtccagat gaaaacaaag gcaaagatcg ggaagaagcg gtcaaggcag
    21601 agattgaaga caatgacaat gcgaatctca gtattaccaa gaccatgaac cgtttaggga
    21661 ccgtggtctt tttggtattg ttcaatattg gcatttcctt ctttgttttt cttctaacag
    21721 ggattatgtt gttctcgcaa attctcttca ttatctttgc gatgtttctg ccaattagtt
    21781 tcttattgtc catgttgccg acttatgaaa gtttaggaaa gaaggcgatt attcgtttat
    21841 tcaatacgat tatgatgcga gcgggtgtta ctttggtgat tacgactgca tttagcatct
    21901 ccacgatgtt tttcaatatt tcggcaacct atccattttt catggttgct tttctgcaaa
    21961 ttgtgacctt tgccggtatc tatttcaaat tgggcgacat tatgagtatg ttcaacctcc
    22021 agagtaatga tagccagtca atgggaagac gcgtcatgcg aaaaccgcaa atgttaatga
    22081 atcgaaaatt gcggcaactc aatcgaaatg tgggacgagc tttggctttt ggtggtggtg
    22141 cagcagtcgg aaataagttg gcaaaggaac aatcaaaatc caaatttaag ccatccggtt
    22201 cttctctacg aaagaattca cgattaccga acgatcacga aatactatct gacttaacta
    22261 aggagaatcc agtttcaaat aataagaaac aatcccgaat gaatttggct gggagaaaaa
    22321 tcggcaaagt tttggatacc caagctttgg tgaaagataa gactaaacaa gtaaaggatc
    22381 aggtaaggaa tacgccaacg aatttgaaat acaatctcca taaaggaatc gaaaagtcga
    22441 aaaaagcacc agaagaattc aagcgcggac tgttccaaga aaaagcggat cgagtggatt
    22501 tgcgagaaaa acaacgacaa cgaagaaatg cgaaaatgga cgaaaaacgc aagatactga
    22561 gtgaagcaac aaatcgtcag ggaaaaagaa gggcgaatat tccaatagat aaagcagctc
    22621 aacctcagaa agatatgatg ctgaaaaaag aagcaccaaa gcggattgtg agagcaaatc
    22681 tgaatcccga aatcaaacga aaactaacta gtcaagaaat catttcaaaa gggaaaagcc
    22741 aatcagcaag taagaaaaat tctttccaac aagtgaaaca gaggtccact ctgccaaagc
    22801 agaccaatca aaaagtacaa aaaagaatga accatcgtcc aaaatcaagg tcaggtgaga
    22861 aaaaatgaac ttgaagaaag tgggtgttat ttcattaact tttctgacca tctcttttat
    22921 gggtattttg atgtgtgtgg gtttgttgtt tggtgaagac agtgagggaa atggtagttc
    22981 tggcacttct cctggcggaa ctagtgtctc agaagaagta ttgaaacacc gtgcaatggt
    23041 ggaaaagtat gccaaagaat cggatataac agagtatatt ccaattcttt tggcgattat
    23101 tcaagtggaa tctggtggca caatggaaga cgttatgcaa agttctgagt cagccggatt
    23161 accacctaat tcattaagta ccgaggcatc catcaaacaa ggctgtctct attttgcgtc
    23221 tttggttaag cgttccaaag aactagggtg tgatcaagat agtattattc aggcctataa
    23281 ctatggtggc gggtatctag atcatgtcgg gaaaaacggg aagaagcata gttttgcttt
    23341 agctgaatct ttttcaaagg aaaaatcagg tggtcaaaaa gtcgattacc ccaacccaat
    23401 cgcgattaag gaaaatggtg ggtggcggta taattatggc aatatgtttt actgtttatt
    23461 agtgaagcaa tacctaacta caactcagtt tgatgacaaa atagtccaag aaatttttga
    23521 tgaggcattt aagtatgaag gaacggccta tgtgtttggt ggctcaagtc cagaaacggg
    23581 gtttgattgt agtggcttga cccaatggtg ttatgccaaa gttgacctga aacttcctcg
    23641 ggtggctcag gatcaatatg atgccatgac tcatattgat ctaaaagatg ccaaaccagg
    23701 tgatttagtt ttctttcatt caacctatga tgccggtact tatgtgaccc atgtgggcat
    23761 ttacgcaggg gagaatcgca tgtttcatgc aggagatcca gtggggtgga cgaatctaac
    23821 ggaaaactat tggcggcagc atcttatcgg agctggccga tacaaataag agaaaagagg
    23881 aaaaagaatg aagataaaaa ttgaacggaa tcaaaaggaa aagagcccaa aaaagaaaaa
    23941 agaacgagtt gtatccgtgg gtaagcatcg aaaaatggtg cttgcccttt ggcttttatt
    24001 gtcttgtagt cttgcttttg gcgtttataa aaatttcacc gccattgatc aacatacaac
    24061 tcatgaaaaa gtggttataa aagagaaggt agtgaatact tctggcgttg aaagttttac
    24121 agaggatttt gcgaaagaat atttttcatg gaaaaataac aaagaagcaa ttgaaaagag
    24181 aatgagtaat ctcgaacagt accttacaga aaacggtctc gctttaatcc aagatatggt
    24241 tcgagcagat attcccacga gttccgaagt tcaatctgta aaaattctag atgttgaaaa
    24301 acactcggaa gaatttgtcg tttcattttt agtggagcaa catattacag agggaaaaaa
    24361 gacgcaatca atttcttctg catatcgagt gacaatcttt gaagatgaaa acaggaatca
    24421 tttagtaact agtttgccaa caatgattgg aaagccagac aaggcaaaat atgaagcaaa
    24481 acaagtagaa aacgattcag aaattgatgc caaaacaacg gaagaaatca ccgatttttt
    24541 agaaacattt tttaaacttt atccatcagc ttctgaaaaa gaattggagt attatgttga
    24601 aaatggtgcg atgcgaccaa tcaatattga cttaaagttt tcggagttag agaatccagt
    24661 ttatctacaa aacggaaaaa attttctagt gagtacaaag ataaaatacc ttgataatgt
    24721 atcaaaaacc actagctact ttcagtacaa attagtatta caaaaaaatg gaaactggaa
    24781 aatcgttgat gttaaataat aatgactcaa tctaatttta ttagaagtta taagtagtgt
    24841 aacttatttc aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccattcggta
    24901 actcgaaaac cgacaattaa gacatagtgc ctttttattt taatccatgt cataatcagt
    24961 ctatatagga aatggttgat ataccatgga attagataca aataatcatt cagtttttct
    25021 tcttggttac ccccttattt tagtagtgaa acattgtaaa catgtcattg atgatgtaat
    25081 gtcagcttat gcaaaaactg cctttgaaag aatttcagaa tcacatcaca ttaccttaga
    25141 tgaatgaaat cgtgataaag aatactttca tattatgctc aaagcacaac ctaaaaccga
    25201 attaacaaaa ttcagcaatg cctaagctca cgtctgataa aatgagactt tctaagggtc
    25261 aaaaatttcc tttgaatacg attattgctt agataaaagt aaaagcctta gaactacggg
    25321 gatagcttgg ttaataagag atatcgttgt tagtaaagac ataggcttgc aagtatgctc
    25381 tattcccaag aatctccccc ttcaagcacc aaggatcgtt aaattcagct aagtggcaag
    25441 taattcacta gacgataatt atctccatat ttcaatctca gcttttccat ttttcatagc
    25501 aaattccgtt atttttatag catctaaaag attcagcttt tccactgatc tttcaccact
    25561 tttcagcttt tttattgtgc ttaaattgat accagtacct tttgatattg aattggaagt
    25621 aaaatcatta agggtttttt ttattaaatt catatccgca attccagcgt tcataagaat
    25681 gaagattctc ctctcataga ctactttgaa aatggtacta cagtggctgt ttatatattt
    25741 cagtaggtta ctgtagtggt catcttatta taattagaaa aggaacctaa atcaaaaatg
    25801 ctaaagaaaa ttttcatagt attaagcctc tcatgactaa agccacggaa ttcttgataa
    25861 aagagtatac ttgcaagcct aaacagcggt atctcttatt aaccaagcta tccccgttgt
    25921 tcctaaggtt cttgatgttt tctaaggcaa tgcttgtatt catagccctt ggtcaaaata
    25981 ttgatactat cgttgacgtt tcgattatga tgtatacggc aaataggaca agtccattct
    26041 cgaattgtga gtgacttctt accatctgtg tgtctacatt ctgaaccaat ttgactagac
    26101 ggaaatcatt tatctatctt aatgattttt tgacagaagt atcgaaaatc tttacataat
    26161 ctgggtgatg tattcttatg cagatgagag cgttggctcc gtattcataa ccagtagcct
    26221 ctaaaacgta gtccatcggt gggataccta gcttgcggtt gccagcggct ttcgcagtct
    26281 tcttgtgatc aaatcaagca tctcattgag cactagtatt ttgaagataa tacttgagtt
    26341 tttcggcttt tagaagttca cttcatcttc catcatccat ggaaatccca ctcacccata
    26401 cgcaggcctc caaaaattct tcaacagtgg ttttttcaga catttttctg aatacctcct
    26461 tgttttttga tacgccagat agtcaggaaa aaggcaacaa aacacaaaac aaagctgcct
    26521 aaaaaggtaa tcttcattcc atataaaaag acatccggcc gatttgcgat atagttagtt
    26581 acttgctggc ccatcttagc agacatcgct tgatataaga ccgtcgtaga aagcgaaatc
    26641 ccgctgacca tccccaaatt tcgggcaaag gaattcatcg agcctgccag tcccaagttt
    26701 tcgatagtga ccgagctcat aaccatcgta ttattggggg actggaacaa ggcattgccg
    26761 gcacccatga tggcagtagc gacaatgtat agccacaaag gagagtccac tgacaaaaac
    26821 atatacatga cctgggttac cgacaataag cccaagccta ctaataccaa aatctccggg
    26881 ccaacccggt cggtcaggta gccgcttaaa ggcgccccga cgaccatcat caaaggaaag
    26941 accatcatca acagcccagc atagctagga ctcaacccgc gagcattttg cagataaaaa
    27001 ggaatcacta cattgacaaa gaagttggaa gaaaaaatca acaaggctgt aatcaagctc
    27061 atggtaaaaa tcttgttctt aaaaataccg aaagaaatca atggagaggc tgctttcttt
    27121 tcaactttta aaaagctgac aaaggcaatc actgaaatta cgaacagtac aagggggact
    27181 ggatggaaaa agccactttc ttgccccaaa aagattgctc caaaaaagct gataatcgta
    27241 gccgcaatgg cagcaaagcc ccctccatcg attgtatggc cgctcatggt gatatctttt
    27301 ggtaagaatt tttcagcaat caacatcgtt acgattccca ccggtacatt gatccaaaaa
    27361 atgtacgccc aagaaaattg ggacaaaatc aagccgccaa gacctggtcc agcaatcgag
    27421 cctaatgaaa caaacgcacc tattgctccc aatgcccgac cacgttcatg tatgggaaag
    27481 acttccgtga tgattccggc atttgtagcc atagtcatac tagcaccaat ggcttgtacc
    27541 acccgtgcga acaataaaat acctagcgaa tgattgaacc cacacaacag cgatcccaag
    27601 acaaagatca gcgtaccaat ccgaaatact ttgattttcc cacggctatc gccaatttta
    27661 ccaaacagca acaaacaaga acagacaatc atcagataaa cggacactac ccattccgct
    27721 tgattcattg gtatgcccat atcatctgag atggtaggta gtgcaatatt gacaatactg
    27781 ccatccaatg tggacatgaa ggtaaacata gatatagcaa tcaaaatcca ccagcgattt
    27841 ttttggactt ttgcatcttc ttgataacta tacataatag gatttccttt ctaatgatcg
    27901 tgtagctaag aacaatataa cctatgtcta ctgcccaaca acttctcgcg actctagcca
    27961 taggaagctt gagtaatttc gatttgatat cctcatgtaa ccatgtaagt tggagatgct
    28021 ttatttgaac ttactctata gtaactagtg ataattcgct ttactttctt ctagcaattt
    28081 ctaaaagtcg acttcattgt tcttcagatt aatagtgtga gctatctcgt gagacgtaag
    28141 caaatttcca ttctactttt tcccaatttt ttcatattta tggtgttgca cctatttgtt
    28201 ttccaatttg cttttcaaat acattgctat gatattcaat tccataggat tatactaaa
//
LOCUS       DAZKMZ010000029        27617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000029 DAZKMZ010000000
VERSION     DAZKMZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.029"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..1953
                     /locus_tag="ACG8M5_001368"
     CDS             187..1953
                     /locus_tag="ACG8M5_001368"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0358629.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2055..3314
                     /locus_tag="ACG8M5_001369"
     CDS             2055..3314
                     /locus_tag="ACG8M5_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0358630.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3461..4762
                     /locus_tag="ACG8M5_001370"
     CDS             3461..4762
                     /locus_tag="ACG8M5_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358631.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4765..5616
                     /locus_tag="ACG8M5_001371"
     CDS             4765..5616
                     /locus_tag="ACG8M5_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0358632.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5629..6444
                     /locus_tag="ACG8M5_001372"
     CDS             5629..6444
                     /locus_tag="ACG8M5_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0358633.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6595..7761
                     /locus_tag="ACG8M5_001373"
     CDS             6595..7761
                     /locus_tag="ACG8M5_001373"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358634.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7991..8638
                     /locus_tag="ACG8M5_001374"
     CDS             7991..8638
                     /locus_tag="ACG8M5_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0358635.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8722..9666
                     /locus_tag="ACG8M5_001375"
     CDS             8722..9666
                     /locus_tag="ACG8M5_001375"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0358636.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9801..11252
                     /locus_tag="ACG8M5_001376"
     CDS             9801..11252
                     /locus_tag="ACG8M5_001376"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0358637.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11273..12109
                     /locus_tag="ACG8M5_001377"
     CDS             11273..12109
                     /locus_tag="ACG8M5_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0358638.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12109..12768
                     /locus_tag="ACG8M5_001378"
     CDS             12109..12768
                     /locus_tag="ACG8M5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0358639.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12894..13583
                     /locus_tag="ACG8M5_001379"
     CDS             12894..13583
                     /locus_tag="ACG8M5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358640.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13589..14545
                     /locus_tag="ACG8M5_001380"
     CDS             13589..14545
                     /locus_tag="ACG8M5_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0358641.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14589..15101
                     /locus_tag="ACG8M5_001381"
     CDS             14589..15101
                     /locus_tag="ACG8M5_001381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358642.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15357..15905)
                     /locus_tag="ACG8M5_001382"
     CDS             complement(15357..15905)
                     /locus_tag="ACG8M5_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0358643.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15994..17175)
                     /locus_tag="ACG8M5_001383"
     CDS             complement(15994..17175)
                     /locus_tag="ACG8M5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0358644.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17411..18919)
                     /locus_tag="ACG8M5_001384"
     CDS             complement(17411..18919)
                     /locus_tag="ACG8M5_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0358645.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19078..19269
                     /locus_tag="ACG8M5_001385"
     CDS             19078..19269
                     /locus_tag="ACG8M5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358646.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19472..20389
                     /locus_tag="ACG8M5_001386"
     CDS             19472..20389
                     /locus_tag="ACG8M5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0358647.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20382..21455
                     /locus_tag="ACG8M5_001387"
     CDS             20382..21455
                     /locus_tag="ACG8M5_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358648.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21603..22121
                     /locus_tag="ACG8M5_001388"
     CDS             21603..22121
                     /locus_tag="ACG8M5_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358649.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22102..22494
                     /locus_tag="ACG8M5_001389"
     CDS             22102..22494
                     /locus_tag="ACG8M5_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0358650.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22570..23505
                     /locus_tag="ACG8M5_001390"
     CDS             22570..23505
                     /locus_tag="ACG8M5_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0358651.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23638..24417
                     /locus_tag="ACG8M5_001391"
     CDS             23638..24417
                     /locus_tag="ACG8M5_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0358652.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24410..24727
                     /locus_tag="ACG8M5_001392"
     CDS             24410..24727
                     /locus_tag="ACG8M5_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0358653.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24971..26581
                     /locus_tag="ACG8M5_001393"
     CDS             24971..26581
                     /locus_tag="ACG8M5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0358654.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26738..27586
                     /locus_tag="ACG8M5_001394"
     CDS             26738..27586
                     /locus_tag="ACG8M5_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0358655.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8938 a         4886 c         5546 g         8247 t
ORIGIN      
        1 ggacactaaa tagtacagga agtagattaa aacttttaaa agttttaagt ctatctattt
       61 ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac atttttgaaa
      121 gcgttgtctt ttgtttctct atcacttata gtgatgacgt catattaaaa cacaaggagg
      181 aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc atttctatat
      241 acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa aaaagtatat
      301 gtgctttgtg gagatccata taccataaca actgaaaaat ggtatcaaaa acaacgacca
      361 atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt gacgagcgaa
      421 acaaggcgct tacagtatgc gttccatgtt gttggggaag ataacacgga ctgtttttac
      481 ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt
      541 cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt aaaagaaaca
      601 gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa
      661 cacactttac cttggggaag taaagatcca gaccgagaag acttcttcgg gggagatttg
      721 caaggtgtga gggatcattt ggattacttg acggaccttg gcgtcaatgg catctatttt
      781 tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta ttatgaggtg
      841 gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc acataaaaga
      901 gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc accacagtgg
      961 caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca catccgtttt
     1021 ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa tatcccatat
     1081 gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc agaagtgcaa
     1141 aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga tggatggcgt
     1201 cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga agcagttaca
     1261 gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca agcttggctg
     1321 caaggggatg aattccatgc agtgatgaat tatgcattca cagattcgat taaagattat
     1381 tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca tcagcaaatg
     1441 ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca tgatactgcc
     1501 aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat ggcctttatg
     1561 tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat gactggtgaa
     1621 gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga tctagagcta
     1681 ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat tatttctgaa
     1741 ggttctactc agtggctaat agtatatgat cgtgaagata agctctattt cacacgggaa
     1801 ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa
     1861 caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag
     1921 atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac aacaagaaag
     1981 cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg ttgaatacac
     2041 aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt
     2101 taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag
     2161 ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat tatataaacg
     2221 aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta gaaaaggaca
     2281 tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga cctgacgtta
     2341 tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg gcggaagtaa
     2401 aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact tatgatggaa
     2461 aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac aaagatttag
     2521 tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac agtcgttttg
     2581 cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact gacttttatt
     2641 attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat ggcacaaatc
     2701 cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat gcaacagatt
     2761 ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga gacttcgcca
     2821 gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg caagcgcaaa
     2881 catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta aataatggac
     2941 agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat gctaaaaaca
     3001 aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa gaaaaattct
     3061 ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca aaggctaaaa
     3121 acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg atgccaaaca
     3181 tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat gcagcatcag
     3241 gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt gaagccatag
     3301 aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga acaggtgctt
     3361 cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc attatcttaa
     3421 aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag ataaaacaag
     3481 caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg ggcagtttgt
     3541 aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat tcattggtca
     3601 gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa gtataccaat
     3661 ggaagatcat tcattattta ttttgattga aggcactttg caattcatca ttacaatcat
     3721 tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga agatgcgtca
     3781 agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag gtgatgaagg
     3841 ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga tcattttccc
     3901 agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata ttccaccagc
     3961 cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt taagctcata
     4021 tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat gtgcaacaac
     4081 tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat ttattaaatt
     4141 caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt ttattactat
     4201 catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc aagtgattcc
     4261 tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa
     4321 ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct atatctatgt
     4381 gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag caaaaatcga
     4441 cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct tcctagtggc
     4501 agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca tgatctatct
     4561 gtttaacaat ggtggtcccg gaagcgtagg cggaggagct ggttctactg atattcttat
     4621 ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg cttcagcaat
     4681 cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta aaaagacaaa
     4741 tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa
     4801 cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat tatcatctat
     4861 ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc ttttagttta
     4921 gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga aacactttac
     4981 gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt acaagtgact
     5041 gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg caaatcaagt
     5101 ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac tgccttttat
     5161 gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat gatctatata
     5221 ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac tgtaccaata
     5281 gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt tttccagatt
     5341 gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt tatgggacct
     5401 tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca gactgttgct
     5461 gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct ttttgctgct
     5521 ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca aaagaacttt
     5581 gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat gcaaacattt
     5641 ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg acgcaaacta
     5701 ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat ggtcatgcct
     5761 gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt tctatccgtt
     5821 cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt atctgaaact
     5881 gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt aggtgtgtct
     5941 ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa ggaacaatgg
     6001 gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta tagcccatct
     6061 ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga aagagaattt
     6121 tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg tcttgtccta
     6181 tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat gacaagaaaa
     6241 agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct gaatgttatg
     6301 ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt tatcttgatg
     6361 ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt caaaacaaga
     6421 ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat gaatgtgaaa
     6481 aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt cttagttgat
     6541 tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa aatcatgtta
     6601 gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt cgaagtaaat
     6661 tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca aatcaaaatc
     6721 aatatccatg aagaaatccc aatgaaagag attacaagta acgcttcttt ggcactagga
     6781 gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt gatcaacgat
     6841 gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg gctgccaaag
     6901 aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt
     6961 aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc ttatttcaat
     7021 acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat cttagacaaa
     7081 ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg aacattgatg
     7141 ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag tgaagaacaa
     7201 tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg ccgtgtatta
     7261 ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg
     7321 ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa agatttattg
     7381 aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg tgcgacaaat
     7441 gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac tgaattagtt
     7501 gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg tcgtgactac
     7561 caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca agtcacgaaa
     7621 gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg tgcagcttca
     7681 tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac
     7741 atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat ttaaaaagaa
     7801 gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg attgtctcta
     7861 aagcttagtt tctttttctt tatattttca taataaaagt ttggttcttt aatttttaaa
     7921 catgacagaa aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg
     7981 gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc
     8041 tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca
     8101 gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac
     8161 aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat
     8221 cgaattgttc caagataaat acccagatac tgcaattact agtttggaat tagatagtga
     8281 ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag
     8341 aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga
     8401 acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga
     8461 ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt
     8521 ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac
     8581 atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga
     8641 atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac
     8701 ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc
     8761 aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg
     8821 gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga
     8881 aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca
     8941 aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac
     9001 caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg
     9061 cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc
     9121 aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa
     9181 gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt
     9241 gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct
     9301 atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa
     9361 ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat
     9421 gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt
     9481 cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt
     9541 acagagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag
     9601 attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt
     9661 tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt
     9721 ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca
     9781 gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa
     9841 aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc
     9901 atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga
     9961 ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc
    10021 aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt
    10081 ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa
    10141 ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa
    10201 gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag
    10261 tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac
    10321 ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc
    10381 catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga
    10441 gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag
    10501 tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg
    10561 ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg
    10621 tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc
    10681 gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct
    10741 cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga
    10801 ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat
    10861 gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac
    10921 caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag
    10981 cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca
    11041 tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac
    11101 cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta
    11161 tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc
    11221 atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa
    11281 aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc
    11341 tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt
    11401 agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg
    11461 ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa
    11521 agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc
    11581 gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa
    11641 atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga
    11701 agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga
    11761 aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga
    11821 tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt
    11881 atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga
    11941 aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt
    12001 aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga
    12061 tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttccc
    12121 ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac
    12181 acattaggga ttggcttcgt gtcgtttctt ttaggaaatt tggtagggtt gcttttgaca
    12241 gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc
    12301 tttagaggca ttcccgcact agtcctgctg tttctgttat atttcgggtt accttatcaa
    12361 ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt
    12421 gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct
    12481 tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc
    12541 tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca
    12601 acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat
    12661 tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa
    12721 aaagtgcaac agtatttaga aaaacgttct atccatcagt caatctgaaa aatagaaatg
    12781 gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt
    12841 taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga
    12901 ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct
    12961 tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct
    13021 tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag
    13081 attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa
    13141 gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc
    13201 gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag
    13261 cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt
    13321 tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaaaag
    13381 atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac
    13441 gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag
    13501 aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt
    13561 tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt
    13621 tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc
    13681 agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa
    13741 aaagcaccat ccaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga
    13801 tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt
    13861 aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg
    13921 acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt
    13981 tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg
    14041 tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt
    14101 tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat
    14161 cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac
    14221 gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc
    14281 tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat
    14341 gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca
    14401 gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga
    14461 acttattagt cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca
    14521 ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag
    14581 gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca
    14641 ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga
    14701 aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag
    14761 tggaagatca aattgctgga acagctgcat tagtctcagg cgtggatccc gtttacacaa
    14821 aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt
    14881 tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga
    14941 ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga
    15001 tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa tttccttttg
    15061 aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca
    15121 aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac
    15181 agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt
    15241 ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg
    15301 tgacatagct tttgtcacga tctctcggct tatcctacga gtattattcg atacatttat
    15361 atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg
    15421 actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc
    15481 gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat
    15541 gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa
    15601 tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta
    15661 atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca
    15721 tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct
    15781 ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa
    15841 atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa
    15901 ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca
    15961 aagcttttaa aagtaacttt gcttttttgt attttatacc agcaagccag tcacaatagc
    16021 acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag
    16081 atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa
    16141 gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa
    16201 attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat
    16261 gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc
    16321 aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt
    16381 gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg
    16441 gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc
    16501 aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat
    16561 ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc
    16621 catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa
    16681 caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg
    16741 tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt
    16801 acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat
    16861 accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact
    16921 tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag
    16981 atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa
    17041 tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc
    17101 ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc
    17161 taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc
    17221 aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat
    17281 tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa
    17341 agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag
    17401 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
    17461 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
    17521 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
    17581 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
    17641 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
    17701 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
    17761 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
    17821 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
    17881 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
    17941 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
    18001 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
    18061 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
    18121 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
    18181 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
    18241 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
    18301 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
    18361 agcaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
    18421 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
    18481 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
    18541 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
    18601 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
    18661 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
    18721 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
    18781 gtacacgttt cgcatgcttg tcgtcaacta acttcccacc aaattctaaa tataatttgt
    18841 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
    18901 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
    18961 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
    19021 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
    19081 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
    19141 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
    19201 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
    19261 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
    19321 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
    19381 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
    19441 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
    19501 tgagccagac gaactagagg agacgaacgc ggatcaggat acaggacaag aatcgtcttc
    19561 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
    19621 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
    19681 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
    19741 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
    19801 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
    19861 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
    19921 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
    19981 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
    20041 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
    20101 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
    20161 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
    20221 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
    20281 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
    20341 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
    20401 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
    20461 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
    20521 aaatacatga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
    20581 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
    20641 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
    20701 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
    20761 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
    20821 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
    20881 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
    20941 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
    21001 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
    21061 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
    21121 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
    21181 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
    21241 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
    21301 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
    21361 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
    21421 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
    21481 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
    21541 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
    21601 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
    21661 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
    21721 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
    21781 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
    21841 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
    21901 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
    21961 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
    22021 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
    22081 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
    22141 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
    22201 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
    22261 tcggaaaaag aagtggtctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
    22321 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
    22381 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
    22441 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
    22501 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
    22561 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
    22621 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
    22681 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
    22741 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
    22801 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
    22861 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
    22921 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
    22981 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
    23041 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
    23101 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
    23161 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
    23221 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
    23281 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
    23341 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
    23401 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
    23461 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
    23521 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
    23581 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
    23641 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
    23701 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
    23761 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
    23821 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
    23881 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
    23941 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
    24001 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
    24061 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
    24121 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
    24181 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
    24241 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
    24301 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
    24361 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
    24421 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
    24481 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
    24541 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
    24601 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
    24661 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
    24721 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
    24781 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
    24841 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
    24901 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
    24961 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
    25021 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
    25081 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
    25141 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
    25201 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
    25261 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
    25321 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
    25381 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
    25441 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
    25501 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
    25561 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
    25621 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
    25681 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
    25741 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
    25801 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
    25861 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
    25921 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
    25981 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
    26041 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
    26101 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
    26161 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
    26221 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
    26281 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
    26341 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
    26401 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
    26461 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
    26521 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
    26581 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
    26641 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
    26701 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
    26761 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
    26821 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
    26881 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
    26941 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
    27001 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
    27061 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
    27121 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
    27181 cgatcgaatc cgagataata cctatgctta tctgattgta gatgatttga acaatttagt
    27241 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
    27301 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
    27361 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    27421 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    27481 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    27541 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    27601 aaaacatacc aaaaata
//
LOCUS       DAZKMZ010000030        27351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000030 DAZKMZ010000000
VERSION     DAZKMZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.030"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..1308
                     /locus_tag="ACG8M5_001395"
     CDS             139..1308
                     /locus_tag="ACG8M5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0358656.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1781..2335
                     /locus_tag="ACG8M5_001396"
     CDS             1781..2335
                     /locus_tag="ACG8M5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0358657.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2508..2945)
                     /locus_tag="ACG8M5_001397"
     CDS             complement(2508..2945)
                     /locus_tag="ACG8M5_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0358658.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2993..3940
                     /locus_tag="ACG8M5_001398"
                     /pseudo
     CDS             2993..3940
                     /locus_tag="ACG8M5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4211..4646
                     /locus_tag="ACG8M5_001399"
                     /pseudo
     CDS             4211..4646
                     /locus_tag="ACG8M5_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4662..5495
                     /locus_tag="ACG8M5_001400"
     CDS             4662..5495
                     /locus_tag="ACG8M5_001400"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358659.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5740..6765
                     /locus_tag="ACG8M5_001401"
     CDS             5740..6765
                     /locus_tag="ACG8M5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358660.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6903..7028
                     /locus_tag="ACG8M5_001402"
     CDS             6903..7028
                     /locus_tag="ACG8M5_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358661.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7053..8168
                     /locus_tag="ACG8M5_001403"
                     /pseudo
     CDS             7053..8168
                     /locus_tag="ACG8M5_001403"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8251..9147
                     /locus_tag="ACG8M5_001404"
                     /pseudo
     CDS             8251..9147
                     /locus_tag="ACG8M5_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9163..9987
                     /locus_tag="ACG8M5_001405"
     CDS             9163..9987
                     /locus_tag="ACG8M5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358662.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10019..11329
                     /locus_tag="ACG8M5_001406"
     CDS             10019..11329
                     /locus_tag="ACG8M5_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0358663.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11477..12736
                     /locus_tag="ACG8M5_001407"
     CDS             11477..12736
                     /locus_tag="ACG8M5_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0358664.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12746..13618
                     /locus_tag="ACG8M5_001408"
     CDS             12746..13618
                     /locus_tag="ACG8M5_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358665.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13630..14460
                     /locus_tag="ACG8M5_001409"
     CDS             13630..14460
                     /locus_tag="ACG8M5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358666.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14500..16683
                     /locus_tag="ACG8M5_001410"
     CDS             14500..16683
                     /locus_tag="ACG8M5_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0358667.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16744..17964
                     /locus_tag="ACG8M5_001411"
     CDS             16744..17964
                     /locus_tag="ACG8M5_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0358668.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            17961..19421
                     /gene="gtfA"
                     /locus_tag="ACG8M5_001412"
     CDS             17961..19421
                     /gene="gtfA"
                     /locus_tag="ACG8M5_001412"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0358669.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19498..20604
                     /locus_tag="ACG8M5_001413"
                     /pseudo
     CDS             19498..20604
                     /locus_tag="ACG8M5_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            20926..21135
                     /locus_tag="ACG8M5_001414"
     CDS             20926..21135
                     /locus_tag="ACG8M5_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0358670.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21128..21277
                     /locus_tag="ACG8M5_001415"
     CDS             21128..21277
                     /locus_tag="ACG8M5_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0358671.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21431..22096
                     /locus_tag="ACG8M5_001416"
     CDS             21431..22096
                     /locus_tag="ACG8M5_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0358672.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22380..23465)
                     /gene="xerS"
                     /locus_tag="ACG8M5_001417"
     CDS             complement(22380..23465)
                     /gene="xerS"
                     /locus_tag="ACG8M5_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0358673.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23799..24032
                     /locus_tag="ACG8M5_001418"
     CDS             23799..24032
                     /locus_tag="ACG8M5_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0358674.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24029..24436
                     /locus_tag="ACG8M5_001419"
     CDS             24029..24436
                     /locus_tag="ACG8M5_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0358675.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25573..26091)
                     /locus_tag="ACG8M5_001420"
     CDS             complement(25573..26091)
                     /locus_tag="ACG8M5_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0358676.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26458..26706
                     /locus_tag="ACG8M5_001421"
     CDS             26458..26706
                     /locus_tag="ACG8M5_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358677.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27209..>27351
                     /locus_tag="ACG8M5_001422"
     CDS             27209..>27351
                     /locus_tag="ACG8M5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0358678.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQS"
BASE COUNT         9366 a         4165 c         5057 g         8763 t
ORIGIN      
        1 gatataacga aatatctaca cagacaaaat gatagcgtaa aatattttgt gtaaatagat
       61 atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt
      121 ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat
      181 aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg
      241 caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca
      301 ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat
      361 ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa
      421 agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg
      481 tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt
      541 tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa
      601 tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt
      661 tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt
      721 gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta
      781 aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac
      841 gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct
      901 agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttcaaa
      961 ttagtttatc aagcaagttc aaaagaagaa gcaaataatc aaataaggtt tatgatagat
     1021 aaatggaaaa agcagtatcc acgagtggtt aaattgctca tgaatcctgc tatattaacc
     1081 ttttataatt tccctccgtc aatcagaaga actatttatt caactaattt gattgaaggt
     1141 tttaacaaac aattaaagaa atataccaaa agaaaagagc aatttcctaa tgaagaatct
     1201 ctggagagat ttctagtatc tcaattcaac aactataatc aaaagtttct atgccgagtt
     1261 cataaaggtt ttaaagaaat acatgataca ttagaatcaa tgttttaact tgatcaacag
     1321 aatgggcttt tccatttaca caaaattatt gacgctcccg acaaaattat gatcttgcgt
     1381 aaagttataa aatattaata aaaatatttt attaatgaaa agcttggtag cacggttatt
     1441 catatgctaa tttatttcac tttatagtaa ggggagttct tagtatattg ttcaaactta
     1501 aatttcatat aatttaatta tgtaaattag ctattctaag aacttctacg gataactgcc
     1561 cgtctaaaat ttaaataaga gcttagaatc ttcatataag taaggttaat ttttttgtac
     1621 aaaaaagtcg gaatattctg agttactgaa atgatataaa atcataaact aaatcccttg
     1681 attttattga ctttcaaaag tttatatata taagttaatg attatggtaa aatatataca
     1741 taatcattaa ctcagaatat ttttaagaag ggaaacaata gtgagctata atgtacaacc
     1801 gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa caagtaatcc
     1861 ggaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta tgtcagatat
     1921 tataggatta aaagttggag atatacgagg caaaagtaag cctattattg ttgaacataa
     1981 aacaggtaag cgaaaaccga tttttataga taatttgaga gaagaaattt tattatatac
     2041 tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg caacaaggtc gacatataac
     2101 aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggac gagatgatat
     2161 aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa ctagagacat
     2221 tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa ggtacattgg
     2281 tataactgaa gatgaaatag gagcatcact tcgtggattt aaactaggtg tataatttaa
     2341 aaatttttat aaacagtaaa gacaaagatt aaaagctatt tttatctaga acgattttat
     2401 ttcataaagt tcgcggaata aaagctgggg ttactggtac ttggtatagg ttcgtgttat
     2461 ataaactaag taattaaata acatttttag gttggaaaag gatgaaatta aaaaaagaga
     2521 atcaatacgg tctcaaaaac gcaaacgctg cggaataaga aaaatactga taactcaacg
     2581 tttagcagac tatctaatag ggatagtctg cttttttcgt ttttgaccat acttttgacc
     2641 ttctgcaaac ttccttgctt ttcctctgta gaactcatga aatctgcaaa acgaattgcc
     2701 gtttcctctg tctgcttttt cgcgatatgg gtgtatatat catccatagt ggttttaata
     2761 tctgtatgtc caagtcgaac atacacttct ttgacagacg caccagattc aaagagcaga
     2821 ctacaatggg tatgacgaaa accatgaagt gtgatatgtt ttaagttatt ctctttaatg
     2881 atgattttca aattgtgatt tacatagtct aaataaaggg gcttattttc ttctgatgaa
     2941 aacaaaagtt gttcaccatc ttttataggt ttgattctca tttttagata ttatgcccgc
     3001 acgtattcat cacatttcta ttgtgaatcg ctttattcga ccgacatttg acttttatca
     3061 taatatactc ggtttaaaat tactaatgaa aacaattaat caagacgatc ataccatgta
     3121 tcacctgttt ttttcagata atcaccaccg tgtgggaact gaactgactt tttttgaagt
     3181 acaagaaggg aacaaccagt tttttggaac caatacgatt gaacgaacga tactaaaagt
     3241 tccttccgaa gcatcattgc atttttggga aatatatttt gaaacacaag gcgtttgcca
     3301 ttatggaatc gaatcatttt ctggacgacc tatcttacgt tttgaagggc cagatgcaac
     3361 acgattagct ttagttccct tacgcgaatt tgaagatatt gatgattatt tcccgtatgt
     3421 cacagaccag atccctacag agcatgccat tttaggaatt gatgccattc aactacgcgt
     3481 acaatattcc gatgcgactg aacgagagtt attgtcggta ggctggtagc acaaagaata
     3541 cgtcagtttc ttttcgggtc atcaacgagt gacaatctta gaaaatcaag acactcagtt
     3601 ctatcaagaa gttcagatta ttcaagatcg tgagaatcca gttgccattg aagggattgg
     3661 tagcgtccat catgttgcgt ttggcgtaga aaataaatct gatttacaac gcatcgacaa
     3721 acaattacaa gagcgcaatt ttataaattc aggaataaaa gatcgtgaat tttttgtatc
     3781 actttattat cgtgacccaa atcaattatt gattgaaatt gctacagggg aaggaaacct
     3841 agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa gaaattcctt tatttttacc
     3901 gcagtactta aatttcattc gtgaacaagt ggagcaataa ttagctaatc aaatccatga
     3961 cgaagagtaa gaggttgact cataagtcaa aaaataaaga aaaacgcatc aaatccattc
     4021 taaagaatgt tgatttgatg cggttcctat ttttataaac tatcattttc gactaataaa
     4081 actacttatg agtcagcctc tctttttttc ttgattttta tacctctttc taggtaaact
     4141 aaacatctgc caaaaatacc gtgtctcatt gaaacattct caactatttc aaaataaagg
     4201 ggagtaccaa atgaacgagt atattgaaat tatccacgca agagaaacaa cttaaaaatg
     4261 tcagtctcaa aattcccaaa aataaaatta gtatttttac cagcgtatct ggttcaggaa
     4321 aatcatcgat tgtttttgat actattaccc aagaagctgg tcgtcaactg aatgacacct
     4381 atgatagttt tacccgttta tttttaccta aatacagtcg tccagaggtt gatgagattg
     4441 ataatctttc tacggcgatt gtggtcaatc aaaagaagat tggtggcagt gcgcgctcaa
     4501 cgttagggac gattactaac atcaatgcgt tatttcgtgt gttgttttca tgttttgcaa
     4561 cacctagttt aggatatgca aatgcttatt catttaatga tcctagtgga atgtgttctg
     4621 tctgtcaggg aatcggtaag acgattgcat ttttaatagg attgctttat ttttcggcta
     4681 ttgataatgc tgattttgta ttttctgatc catttaccgc agaagttttg tctggaattg
     4741 aggaacaggc aaagaatcat ggttattttg tattggtgca taatgttaat tcagcagaag
     4801 atgttaaaac tattcagaga aattggcgtt ttgaggggtt cattgttgtt ggtgtacgtg
     4861 taaaagaatt tttggaatta aataacgttt ttgatggtcc tgttgtttat attgatacgc
     4921 atctttcaaa agagacatta agagaacttg aacaacaaat gaatcgttta tttgtaaata
     4981 caaatgatta ccaagctagt acattggcag tggaacattt gattcattat ggtcatgaga
     5041 aaatagcgtt tctttctttt gactacattg taggtgaacc aagtgttatt caagaacgac
     5101 atcaagctta tatcgataca ttaaagaaat atcatttaac ctttaaatcg cattggatat
     5161 ataacaactc tgaatttgag cgaatttatc aagaattagc tgaatttact gcaattgttg
     5221 ttactggaga ttatttagcg gctaaattag taaaattttt aaaaaataag aataaacgag
     5281 tgatggaaca attatcgatt attggatttg atgatattac ttttgcagaa ttaatggatc
     5341 ctgcgttgac aacaatacga ctaaatccag gtaacaaggg ggcgattgct tttcaccaac
     5401 tgattggact ggccaaacaa gaagaaataa aaaatcaagt actactatta gagggagaat
     5461 tgatttctcg agactctgtt cacaaaatca aataaaaaag ctctgtgaaa taagtatagt
     5521 taacaataaa atatccgaac gatagagaga atgaactgtg atctagcagt tcctcgtcgc
     5581 aaattcacta gaattagcat tatttactct aaattgttcg gatatttttt cttgttttta
     5641 attatgatta cagtgcctat ttttaacgaa atttgcttat tttcaaaatg tatgattata
     5701 tgttagtaaa cactttcaaa tatgaaaaga ggagctatta tggtaggaat tagggatgtt
     5761 gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga ggatccgaca
     5821 ctttcagtaa ccgatgaaac caaacgaaag attgcagaat cggttaattt ttatcattat
     5881 gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg cattaataac gacagtttct
     5941 gaagtagatg agttggaaga tccctatttt cgaacgattc gcagagggat tcaagtcgaa
     6001 gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac accgttggag
     6061 caagacgatt tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct atcttctgtg
     6121 attgaagaag tatcagcaat taattcgaat gtcgtagtga ttgatgaccc tagtattgaa
     6181 gctgaagtag atgcagttta tactgattta acaaaagcaa cgaatcagca tttagatcga
     6241 ttgtataaaa aaggacaccg aaacattgcg tttatcggag gaggacgtgt tcgtttagat
     6301 agccaaggaa ataaacacgt gtcagatgat gattatcgaa aaatagccta tgagacgtgg
     6361 atgaaacaaa aaggattaga aaaatatagt caaatttatt tagaaggctg gaccacactt
     6421 gacggaatgc aggcagcaga tttacttttg aaaaaacatg ctaaagataa gttgccgact
     6481 gctatcatgg taggaaatga tcctattgct gtgggggttt atcgttcact tcaaaaaaat
     6541 ggtctacata ttccagaaga tatttcgatt gtgagttttg ataatattga agttgctgaa
     6601 tttttaacac ctgcattaaa tacggtgaat gtgaatacag aagaaattgg aaaactagca
     6661 gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa agtgattgtt
     6721 tctaataata ttattgttag agaaagtgaa aaaaatatcg attaaaaaag taaacatcca
     6781 aaaaagggtg tttacttttt tgtttactaa tatttttagt aaaaaatctc gacaaaacat
     6841 taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca ggaggaatta
     6901 agatgaaaca tttcaaaaaa atagctttaa cagggatagc tgttttaact gggacattac
     6961 tcgcagcatg tggtggggga gatagtaatt ctaaagacgg gcaagtaact ttaagctatg
     7021 cgagttgaga tactgaccaa gctccaggac taagaaaaat gttagatgaa tttgaaaaac
     7081 aaaatcctga tatcaaagta gaaatggaga caacgccttg ggatcaatat tgggttaaat
     7141 tagaagctgc aacaacggga ggtaatatgc cagatgtagt aaccatgcac tctagcgaat
     7201 cctataaata tatgtcacag aatgctttga tgggacttga tgatgttatc aaagaaaatg
     7261 atattgacat gggaaacttt actgaaggaa ttgctgattt ctatacgctt gaagacgaac
     7321 tatacgcaat ccctaaagat gcttctgtag tggggctatg gtacaacaaa gaattatttg
     7381 ataatgcagg tgtcgattat ccagatgaaa catggacatg ggaaacactc agacaagctg
     7441 caattgattt aacagatgaa tcaaaaggca tctatggttt tgcatcacat attggaacag
     7501 agactggcta ctggccattt atttatcaaa atgggggaga agtatttgcc gatgacaata
     7561 caaaatcagg tttgaatacc ccagaagcac aagaagcttt agctttttat cgagatattg
     7621 cacttgttga tcatgcttca ccaacaatta cagaattaca agatcctgat aagttaagtc
     7681 gttttcaatc aggtagatta gcaatgatga ttgaaggaaa ctggtttgca ccgggatttt
     7741 tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt gttacctaag ggaaaagtta
     7801 atgcaacaat tacaaatgga cttggttggg ctgccagtgc gagtacaaaa tacccagaag
     7861 aagtgaaaaa actaatggca tttttagcta gtgaggacgc caataaaatc caagcggaaa
     7921 cgggggcatc aatcccagca ctaaaaggct ttgaagacga gtgggctgct tcttcagata
     7981 caattgattt gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt ccatacaata
     8041 aagatggatt acgagctgaa caagcagaag ttcaaattat taaccgtatt ctgtctggtg
     8101 aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg atattgagcg
     8161 gaaattaaat tagttaatta tacaatatac aggcgaaagg attctatttc gtctgtatat
     8221 taatggatga ataccaaaaa gggggatatt atgaaaaatc gaatttttgg aaaatatcgt
     8281 aaagatcaag tgatatgggg ctatattttt atttttccaa cggcattagg attaatttta
     8341 ttaaatatat ggccagcaat tcaaacagca attttaagtt ttcaaaaaac ggtagggttc
     8401 ggtgatactg aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga cggtgaagtt
     8461 tttcagtctt tattaaacac attaatttat gcagtggttt cgatacctag tattgttgta
     8521 ttatccttgt tggtggcagt tttaatgaat agaaaagtta aaggattgtc gatttatcgg
     8581 acaatttatt ttctaccagt cgttgctgca ccatccgcag tcgcaatgat ttggcgctgg
     8641 atgttcaata atgattatgg actgattaat aacatgttgg ctaaaatagg tctcgatgga
     8701 ccagcatggt taacggatcc atcgattgcg atttattcta ttatcattgt aggagtttgg
     8761 agtgctattg gttataacat ggtgttatta ctagccggct tataagaaat tcctaaagat
     8821 tattatgaag cagcggaaat tgatggtgct agtccaatcc agcagttttt caacattacg
     8881 ttaccattag ttacaccaac attatttttt gtagttgtaa caacagttat caatgctttc
     8941 caagtatttg atgttatttt tatgatgatt actcctaata gtaccgccat gtttaaatca
     9001 caatcattag cctatttatt ctataaacat tcatttattt taaatgacaa gggatatggt
     9061 tcggcaatcg ttatgttcct tttagtgatt atattaatta ttactgcaat tcaaatgaag
     9121 ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa taaatttttt
     9181 tcaaatgcgc caatccatat catgcttatt ttaggtggta ttgcaatggc attacctttt
     9241 atttggatga ttttaacttc agggaaaacg ttggttgaat ctacccaaat tccacctaca
     9301 attttccctg agaaatttca gctaagtaac tatcaacaag tacttgattt attaccattc
     9361 atggcttttt atatcaatac atttttaatg gttgttggac gagtgattgg ctcggtttta
     9421 ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa aaatatcctt
     9481 ttcagcttag tacttattca aatgatgatt cccggttcca tttttattac tccacagtat
     9541 ttattagcac aaaaattggg tattttaaat acaattccag cattaatatt accaggggta
     9601 gttagtgcgt ttggtacgtt tttattaaga caatttttct taggtttacc tgatgaattg
     9661 gaggaagcgg cctatttaga aggtgccaat atttggacga tctttttcaa aattatgttg
     9721 ccgcttgcta aatcaggatt ggttgcactt ggaatattca cagcgttgtt tgcatttaaa
     9781 gatttaatgt ggccattgat tgtcaatact tcaattgata agatgccttt agctccggga
     9841 ttagcaaact tacaaggaca atatgccacg aacttcccac aattaatggc aggttcgtta
     9901 ttagcaattt ggccaatagt tgtgatattc atcgttttcc aaagaaaatt cattgaagga
     9961 attgctacaa caggtagtaa attataaaac tattaattag tgattggaga gtaagtacat
    10021 gacaaaaatt acatttattg gtgcaggaag tactattttt gcaaaaaacg tattaggcga
    10081 tgcgatgtta actccaagtc tacaagatgc acatattgca ttgtacgata ttgatgaaaa
    10141 acgactaaaa gaatcaaatt taatgcttaa aaatattaat aaaaattcga atgaaaatcg
    10201 tgctgatatt gtttcttatt atgatcgcaa agaagcgtta agagaggctg attttgttat
    10261 caatgctatt caagtgggtg gttataaacc ttcaacagtg attgattttg atattcctaa
    10321 aaaatatggg ttgcgacaaa ctattggtga caccatgggg attggtggga tttttagaac
    10381 cctacggaca ctaccagtaa tgttagactt tgctaaagaa atggaagaag tttgtccaaa
    10441 tgcttggtta ttgaactata caaatccgat ggcgatgtta accatgggtg ttttaaaagc
    10501 gacgagtatt aagactgttg gtttatgtca tagcgttcaa gtatgcgtac cagaattgtt
    10561 tgagcattta ggaatagccg atcaatatga cttaaatgac tttcaatgga aaattgcagg
    10621 cattaatcat atggcattct tgttggaaat tacaaaaaat ggggaagatt tctatccaac
    10681 aattcgtcag ttagctgcag aaaaaccaaa tccacatcca gattctgtac gttttgaatt
    10741 gttgaaacgt tttggttact atattacaga atcaagtgaa cacaatgcag aataccatcc
    10801 atactttatt aaatcaaaat acccagaatt gattgacgaa ttagggattc cgttggatga
    10861 atacttacgt cgttgtgaac aacaaattaa tgattgggaa agcatgcgag aggacattgt
    10921 aaataatggg gagttaacac atacgagaag ccgtgaatat gcttcatata tcatggattc
    10981 tattacgaca ggaactccgt cagtgattgc aggaaatgtc ttaaataaag gcttaatcac
    11041 aaacttaccg acagatgttt gtgtggaagt accttgttta gtcgataaaa atggcgtgca
    11101 accaacctat gtcggtgaat tgccaacaca attagctgct ttaaatcgga cgaatatcaa
    11161 tgttcaagaa ctgacagtag aagccgcttt aacactagat aaatcaaaaa tttatcaagc
    11221 agcgtattta gatccacatc tatcatctga attgtcttta acagatatca aaaatttagt
    11281 ggatgattta atcgaagcac atggggacta tttacctgaa tataattaaa aagaagctga
    11341 aagagtaagg aattttcaat tccttactct tttcttagtt aaatttagta aaaatattga
    11401 caaaacactt agtaaacagt tacattagtg ttaacaaaaa gaaaacgcat tcaaattgta
    11461 taaggaggag cataaaatga agtggtctaa aaaaattatt ttagcttgta tagcactagg
    11521 agcaacgatc tctttgactg cttgtggaaa aaatcaggaa gatgcaagcg gtaaggtaga
    11581 aatagagttt tttaatcaaa aaaaagaaat gactcaaacg attcaggaga ttgctaaaga
    11641 ttttgaagca aaaaaccctg atattcatgt aaaagttgtg gatgtaccga atgctggtga
    11701 agtaattaaa acacgtatgc ttgcaggcga tgtaccagat gtcattaatc tttatcctca
    11761 aagtatagaa ttgaaagaat gggcgaaagc tggttattta gaggacttaa ccgaagaacc
    11821 gtatttagag aatatcaaaa atggatatgc acaacgtttt gctattgagg atcgtgtata
    11881 tagcatacca ttaaccgcta atgtctatgg tttttactat aacaaaactg cttttgagga
    11941 aatggggatt caagcaccag aaacttgggc agaatttgaa aaaatagtag ctgagattaa
    12001 agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa ggctggactt taaatggtta
    12061 tcaccaacta gctatagcga ctgctgctgg tggtgaagag gaagcgaaca atgtttggcg
    12121 tttctcagaa gttaatggaa ttaatgctga aagcaaagaa atgcaaaaag attttaatcg
    12181 attagatttg ttaagagaac caaaagcttt gcaaaacaac tggcaaggtg ccggttataa
    12241 cgatacagta gttactttta caaaaggtga tgcgttgatt atgccgaatg gttcatgggc
    12301 aatgccgatg attcattcac aaaatccaga ttttgaagta ggaacttttc cttttccagc
    12361 agatgaagca ggtcaaagtc taacgattgg cgcaggagac ctagccttat cagtttctgc
    12421 aacttctcaa cataaagaag aagccaaacg ttttgtagaa tatatgacaa cacctgaagc
    12481 gatgcagaaa tattatgatg tagatggtgc accgtgtgct gttgagggcg ttatcgagaa
    12541 tactcaggat tcaccattaa gtggtttaac tgaacttgct tttactgatc gtcatttagt
    12601 ttggttagcc aaagattgga atagtgaaaa tgatttttat accttaacaa caaattattt
    12661 acacaatggc aatcaaaaag ccatgattaa cgcattaaat gctttcttta atccaatgaa
    12721 agctgacgga aggtaggaaa ataaaatgaa aataaaagaa attttaaatc gttactgggc
    12781 aatcattttt gtagcaattc caattatttt gcagatgatt tttttctact ttccaatggt
    12841 tcaaggtgct ttttacagtt taactaattg gactggtctg acttataatt ttgattttgt
    12901 gggtttgaac aattataaga ttcttttttc tgatccgaag tttatcaaat cgattggctt
    12961 cactttaatt gtgacgcttt gcatgattgc tggacaaatt ggttttggta ttttgattgc
    13021 gcgggcgttg aacgcaaata ttaaagggaa aacattcttt agagcatggt tcttttttcc
    13081 agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa caaattttta actatggttt
    13141 accaaaaatt ggtagtatgt taggcattga atttttacaa accagtcttt taggaacaac
    13201 atggggagcg atttttgcag taatttttgt tttactttgg caaggaattg ccatgccagt
    13261 catcattttc ttagcgggtt ttcaaagtat tccacaagaa attattgaag ctgctagaat
    13321 ggatggggcg aatgataagc aaattttttg gaatattgaa atcacctact tattaccaag
    13381 tatttcaatg gtatttatta tggcattaaa aggtggctta acagcatttg atcaaatttt
    13441 tgctttaact ggtggtggac caagcgatgc cacaacatct ttggggttac ttgtttataa
    13501 ttatgctttt aaaaacaatc agtttggtta tgcgaatgct attgctttag tcttatttat
    13561 cttaattggg attgtttcac tggcacaatt aaaattatcg aaaaaatttg aaatctagga
    13621 ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta aatacttgct tttattatta
    13681 gggggcttca tgattttagt tcccttagtt tcaacgctat tcggttcatt taaaacaaca
    13741 cccgatatta tgaataattt ctttcgtttt cctaatccag cgactttgga aaatttccga
    13801 agattgttag cggatggaat tggtcattac ttttggaatt cggcaatcat cacaatcatt
    13861 gcagtagttg ctgtgacagc aattattcct atggctgctt attcactagc aagaaaaatg
    13921 actcgtggga aagcatacgg attgatgtat ttatttttaa ttttaggaat ttttgttcct
    13981 ttccaagtaa ttatgattcc gattaccgtt atgatgagta aaattggttt agcaaatatg
    14041 tggggattaa tcttactcta tctcacttat gccgtaccac agacactatt tttatatgtt
    14101 ggttttatta aactaaatgt gcctatcagt ctggatgagg ctgctgaaat tgatggggct
    14161 agtcacttta caacttattt caaaattgtt ttcccaatgt taaaaccaat gcatgcgacg
    14221 actattattc ttaatgctct atggttttgg aatgatttca tgctaccatt attaatttta
    14281 aatcgtgatt ctaatatgtg gacactacca ttgtttcaat ataattatac gggacaatat
    14341 tttaacgact atggtccaag ttttgcttcc tatacagtag gaattgtcac gattacacta
    14401 gtttatcttg ctttccaaag aagtattata gctggaatga gcaatggagc agtgaaataa
    14461 ccaataaatt attctcatta taaaggagtt cataaactta tgattatatt tgacaatcac
    14521 acacaaacat ttcatttaca aaataaaaaa attagctatc tattaggaat cgaagaaaat
    14581 aattatttaa cgcatcttta ttttggaaaa aagattacgc actattctgg tgggtatcgc
    14641 tacccccgaa cagatcgttc cttctcaccc aacaatgcaa atgcgcaaga tcgtttatta
    14701 tctttagaca cattaccatt agaatttcca ggatatggac atggtgattt tagagaaccg
    14761 gcgcataata ttaaattagg aaacggtagt cgtgtaaatg attttcgtta tgatagctac
    14821 gaaattcaaa aaggaaagcc cagcttagcg ggattgccag ccttatatac ggaagatgat
    14881 gatgaagctg aaacgctgat tattcaattg aaagacgcga ttagtggatt gactttacag
    14941 ttgttctaca caatttatca aaatcacagt gtaattattc gttctacgca attagtaaat
    15001 aatagtgatg aaacagtgga gattaaccgc ttagctagtc aatcaattga ttttccaaat
    15061 cgtccactcg agttaattca tttaaatggt acctgggcaa gagaacgtca aatgactaga
    15121 gaaaaaattc atacagggat aaaagtatta gatagtaaac gcggatcaag cagccatcaa
    15181 caaaatccgt ttgttgcttt agtagatgcc aaaacagatg aatttcaagg agaggcatat
    15241 ggatttaatt tagtttatag cggtaatcac gaaacggtga ttcaaaaaga tccatttgac
    15301 caaacacggg tcattatggg aattaattca tttaattttg gttggcaact aaaaactggc
    15361 gactcatttc aaacgccaga agtcgtgatg gtttatagta atgaaggtct gaatacgtta
    15421 tcacagacgt atcatgaatt gtataatcac cgtttaattc gtggaaacta tcgtttaaaa
    15481 gaacgtccca cgttaatcaa caactgggaa gcaacgtatt ttgattttaa cgaagaaaaa
    15541 atcatgaata ttgtcgatga tgcgaagaac ttgggcattg aaatgtttgt tttagatgat
    15601 ggttggtttg gcggtcgtga aaatgacttc acttcattag gtgattgggt tgaaacagaa
    15661 ggcaaacttg cttgtggttt ggaaaattta gccaagcaag tgcatgataa cgggatgaaa
    15721 tttgggattt ggtttgaacc ggaaatgatt tctgaagata gtgaactatt tcgagcacat
    15781 ccagattggg cattgggtat tccaggtcgt gaccgctcaa ttagtcgaaa tcaatatgtt
    15841 cttgattttt caagacaaga tgttcgtgat aatatctatc aacaaatgac ggcgattttt
    15901 gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc gaaatatgac agaagtctat
    15961 tctgtcttgt tggactcaac gatgcaaggt gaagtggctc atcgttatat gttagggtta
    16021 tatgaatttt tagaaaagtt aacaacgaat tatccaacta ttttatttga aagttgttcg
    16081 ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca tgccacaaac ttggacgagc
    16141 gacaataccg atgccgtggc tcgtctgaaa attcaatatg gaaccagctt agtgtatcca
    16201 atttcaagta tgggtgcgca tgtttcagca gtcccaaatc atcaaacagg aagagaaacg
    16261 agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt ttggttatga gctagatcta
    16321 acgcaattaa cttctgaaga aaaagcagaa attaaagaac aagttgcctt ttataaaaag
    16381 catcgccaac ttttgcaata tggaactttt tatcgcttgg aaagtccgtt tgaaggcaat
    16441 gatactgcat ggatgtttgt ctcaaaagac caaacagaag ccatggtctt ttatttccga
    16501 gtattagcag aagctgcgca tccattagtc acattgaaat tagcaggatt agcagaagaa
    16561 gcaacttatc aaatggaaga tgtaacattt gctggcgatg aattgatgaa tctcggtttt
    16621 tatattgact cagaactaca tggtgattat gcgacacaac gcttttattt gaaacagata
    16681 tagtagcttg aatttacaat ataagtgcaa caccaaaaga gactcattcc aaacgaagaa
    16741 aatatgtttt atttacacgc atttactaaa aagcagcctg atttgaattg ggaaaatgaa
    16801 gaagtacgtg aagaaatcta tgcaatgatt aactattggt tagataaagg actaggcgga
    16861 tttagaatcg atgcgatatt aaacatcaaa aaaaaactag agtatggcat ctttgaatct
    16921 gatggagaag atgggttagc cttcattggg ccctggattc ttaatcagtc gggcattgaa
    16981 gtgtggttaa aagaaatgag agagcggaca tttaaaccac ataatagtat gacggtagca
    17041 gaagccgatg ttcctaatga acgtttagat gaatatattg gtgaagatgg ctattattcg
    17101 atggtttttg attttagtta tacggatatt gatgtacctg aaactggtga atggtttaaa
    17161 gatagccaat ggacatggac ggatatgcgt accaatattt ttacgaatca attagttacg
    17221 caagataaag gttggggcgc cttatattta gaaaaccacg atcaaccacg ttcaattaat
    17281 aaatatattc cagaagaatg gatcaatgat tatagtaaaa aaatgttggc gacgcttttt
    17341 atgttactaa gaggtacccc ttttatttat caaggacaag aactaggaat gaccaacata
    17401 gaaatggcat cattagaaga ttttgatgat gtagctacgc attctcagta taaacgggca
    17461 ttagaatatg gattggcacc agagcaagca ttgaaagctg tcaataaacg aagtcgggat
    17521 aattctcgca cacctatgca atggaatacg caaaaaaatg ccagattctc aacttctgat
    17581 aatgtatggt taaaagtaaa tcctaattat ccagaactca atgcagaagc tcagttaacc
    17641 aatcaacagt cggtattcaa ttattatcat caattaatta aattaagaaa aaatagtgta
    17701 tacaaagacg tgctaatcta cggacaattt ctaccagtga aagataaaaa tgaacatatt
    17761 ttgctttatg aacgccaatt agaagataaa atcgtattag ttatcttaaa cgttacagca
    17821 acagaacaag aatattatgt agatgaacag taccaacaag tattaatcaa taattatgaa
    17881 gatgttttat tagatcaaag tcgaaaattg cgtttacgac cgtttgaaag tattgtatta
    17941 gctaattatg gaggacaatt atgaaaatta aaaacgaagc aatgctcatc acctatgcag
    18001 atagtttagg aaaaaacatg ggcgaattaa aacaagtctt agacaaacat ttacaaggtg
    18061 ttattggtgg tgtacatctg ttgccttttt ttccatcgac aggtgatcga ggctttgcgc
    18121 cgagtgatta tacgacagtt gatccagctc ttggaacatg ggaagatgtg gaagcattag
    18181 gtgaagatta ttatttgatg tttgatttta tgattaatca catttctcgt gaatcaaaat
    18241 ttttccaaga tttcaaaaaa aaccatgagc agtcaccata caaagaaatg tttatccgta
    18301 ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga tattgattta atctacaagc
    18361 gtaaagacaa agcgccgttt caaacggtag aatttgcgga tggtacgaca gaagaggttt
    18421 ggaatacctt tggggaagaa caaattgatt tagatgtgac caaagaagtt gtgaaagaat
    18481 ttattcgaga aaccatcaaa gatatggcga accatggctg ttctttaatt cgattagatg
    18541 cgtttgctta tgcgattaaa aaattagata ccaatgattt cttcgtggaa ccagatattt
    18601 gggaactttt agacgaagtg cgtcaagaag cagcgaaata tgacgtcgaa ttattaccag
    18661 aaatccatga gcattattcc attcaaatga aaattgccaa tcatgattat tatatctatg
    18721 acttcgcctt gccaatggtc actctgtatt cactttatag tggaaaatca gaacgtttag
    18781 ccaattggtt gaaaatgagt ccgatgaagc agtttacaac attggatacg catgatggaa
    18841 ttggtgttgt ggacgctcgt gatttattaa cagatgaaga attggattac acctctgagg
    18901 aattgtataa agtcggggcg aatgtgaaga aggtctattc aagtgccaat tacaataatt
    18961 tagatattta tcaaattaac agcacctatt atagtgcatt aggcgataat gaccgtagct
    19021 atctgttagc acgtgcgatt caagtatttg cgccagggat tccacaaatt tattacgttg
    19081 ggttattagc aggaaaaaat gatattgaac tgttggaagc aacgaaagaa gggcgtaata
    19141 ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga agtgcaacgg ccagtgattc
    19201 aacaattatt tgcactttta aaatttagaa atgaatctgc ggcgtttgat ttggatggaa
    19261 cgattgaagt agcaacgcca tcagaatcag aaatagtgat tacacgtaaa aataaaaacg
    19321 gtagtgatag tgcaacatta acggctaact tggatacgaa agaatttatc attgagtcta
    19381 aagatataaa aattttcact agtgttgcat taaaatttta acctattcca tagaacaaaa
    19441 aatcctttat actagcactt ggatgactta tccaagacca ataaaaagga ctcatacatg
    19501 gataagtata aaacaaatac agcatttaat aaatggtttt ctgccataaa attaggaaaa
    19561 ctttctgtca actcccaaaa atgtattttt gattacaatt cgtatcgtaa aaaactgtct
    19621 tttgaagctg tgctcaagct ctttctctac gcagttaatg aagaaaaaga aagtttgcgt
    19681 gatttgagta cagctcttat cgaccgatcg ctccaactag aaaccgatgt gtcatcgatt
    19741 agtcataccc aattgtctcg agcatttaat gcacttgaac ctaaagtgtt agaagaaatt
    19801 tttcaacaac tgttagaaaa agttcaacat caaatcaaac caaccaagcg aaatcatctg
    19861 tatctgattg attccagtac gttttcgctt agtttaaaac gtcataagtg ggcgactttc
    19921 cgtcaaacaa aatcaggtgt caagttacac ttaaactatt gttatatgga cgatactacg
    19981 atgtatccaa ctgattttac cttaacaaat gcccatgaac atgatctcaa tcaactacca
    20041 gtattggtga atcaaccaga ggcaacttac gtgtttgatc gtgggtatta ggactttgaa
    20101 aagatggatc agatgcattg ggatggctat ttcttcgtca ctcggattaa aaaaaacacc
    20161 aaagttcacg tgatagatac tctggaaaca tcgccagaaa cagaaattct acgagacgag
    20221 ctggttcgtt taggatctaa aacctatcta accgccaatt tccgactagt cactgttcaa
    20281 gataagaacg gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc ttcaaaagaa
    20341 atctctgata tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca catcaaacaa
    20401 cacatgacca ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa tcaattgatt
    20461 ttaacgatga tttccgctct cctcacgttt ctcatcaaat tggagacgaa aacggaaaaa
    20521 tcagtgttcc aaatcaaacg attctttcgt tatttactgt ttcagccctt tgaatgttgt
    20581 ctagaaaaat taattcccac atgagaaaaa agaaatttgg ctcgttactt ttggttttca
    20641 gttgaataac agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag cacagttttt
    20701 tttgacttat tttcagagga aatagttttc agaatattct aaaatgtttt atgcaacact
    20761 agtgatattt tttagaatta gatttctaat gtgcattttt tgagtattaa ggagcaagta
    20821 tacaaagatt tataaagctg attaaatttg aataaaaata tgcaggaacg ctactatttt
    20881 tagtagtgtt cttttttata tttgaataaa aaatgtggtg gttaaatggt ggttataaca
    20941 aaaaatgaat taatgaaatt aggttatggg aagtatcagt ctgagagaat catacgacag
    21001 gcaaaagcaa ttatggtttc aaaaagctat acctattacg aaaatagaag attaggacta
    21061 gtacctgttg aatctgtaga agaagtttta ggtatgaaga tagaggtaga ggaggatttt
    21121 cagaatcatg gttaatttta aaaaagataa aaatgggaaa tatcatttta ctgctagttt
    21181 gggattcgac aaacgaacag ggaaaagagt acaaaaaaac gtagaggggt taaaacagtt
    21241 aaagaagaaa aaaagcgtat accgaacttg tttctaatta tggaaaagaa gcagaaactt
    21301 ctcaaagtac tatgttgttt gaggagtttt tttataaatt attcctacct tattatcaag
    21361 ggaaagtaga agaaagaaca tataaaaatc ggttatctac aatcaaaaaa acttttctta
    21421 tttctttaaa atggagttaa gagaaatacg tccaattgac attcaaaagt ggcaaaataa
    21481 tttattgagc aaatataaaa attcttatgt aagaaacatg tatgggattt ttcagatgtc
    21541 attagatcga gcagttgttt tgggactact tgaaaaaaat cctgcaaaag ttattggaaa
    21601 tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa gaagaattta caaaagttat
    21661 ttcaactttt tatattgatg atttgtacca acattttggt tttatttgta tgtggttact
    21721 ttttatgacc gttatgcgtt tgggagaagc cactgcatta acttggaaag atattgattt
    21781 tgaaaataaa aacttgcgtg tgaataaaac actctattat aagagagcca aaacctatga
    21841 gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc gaactagacg acaatacaat
    21901 taccttttta aaagcttgga aggagcgtca agtttcaata ggaggcatgt cgtttgtact
    21961 ttcttataat ggaacaccta ctcagaaata tacaatccgt catattatga gaagacatgc
    22021 aacgatcgtt ggtgttcata taatccgcat acacggatta aggcatagtc atgcttcttt
    22081 gcttatatca caataaaaag atgcacttcg aattaaagaa aggttaaaac atgctgatgt
    22141 acaaacgact ttaggaactt atggtcattt gtatctaaat tcattgagag agataacaaa
    22201 tgatcccaca gattatatcc aaactaattt ttctaaaatc aaaccgacac ctatttacga
    22261 taataaaaaa taaatgataa acaaaagtag gatcacacca aaagaaacac actagaaaaa
    22321 ctataataca ggcttttcta gcgtgttgtt tcttatttcc gcctatttat acattatact
    22381 cataaagcat ttaatgcatc gatcgtagcc gcattgtcta tatgggtata taaagttgtt
    22441 gtattggtac ttgaatgtcc caattgttgg gcagttaata ccaatgaatt tgtctgttgg
    22501 tagagtctag tagctaatgt atgacgcagt ttgtgagggc tgacttgaat tctaaagcct
    22561 tttgaatatt ttgctaccat ccgttcgatt gcaataccgg atatacgctt ggcttctcct
    22621 tggtaaattg ctagaaataa agctttttcg ttttctgtta ctttatatcg ttctttccga
    22681 atagctagat aattagctaa atattccatt gcgaatgctg ctatattgac agcatctctt
    22741 ttatttcctt ttctagttac taccacatta ttcttatcta aagataggtc atctaaatta
    22801 atatttataa cctcggagag tctcaatcca gatcctaaaa tcagtgcatt gatcgccagg
    22861 tctctttcta catctcgttg ataatagatc aaagctcgtt tactcaaagt atttttatat
    22921 tcattggcaa tataatctag ataattttgt gtatccttat ctagaaataa ttttttctca
    22981 atggcattgg cacgtgcagc taacgtagtt ttatctgtga tggttgctac ttgatccata
    23041 ggattattgt ctaagtatgg ttttcctaga ggattattgg aacttttcga aagatattta
    23101 aatagaacct ttaatgccgt gatatttcta ttgatagtag aagtagatag acctaagtta
    23161 ttattatctt tgagcttagt ttttgttgtc tcttttttta cattttcttt tgtgcgacgt
    23221 ttcaaatgag atttgtacaa gtttaggtca taggcagtta actgtcttat cgaggtgata
    23281 gaagtatcag gaacgataga ctcactcaga agccattcat aaaagagctt ataatttttt
    23341 agatattcta aagtggttcg aggtgattga tctttatcct cacgatcttg aacaaaatct
    23401 tttatataat cgggcatatg aagtatgatt gtatctattt tttgatatat ttctgtattt
    23461 ttcatgtcat tcatcctttc aataagtagg ggagaagagt atctctataa ttggactata
    23521 ccaattatag aatgttttgt caattggtat gctctaaaat ttattaattt gtttcaaaaa
    23581 aagcatataa acaggtatac gtttaataat ttaaaataaa tggtatacat aaatagggaa
    23641 ggaaatagaa gggagcaatc taaatttttt gaattttctt aattaattat atagaaagaa
    23701 caatctttct atagagttag acatgagagg gactatgctt gatttaaaat caacaagcgt
    23761 atatactgta tatacaaaga aaaaggaggg agaagactat ggcaattaaa gaaagaaaat
    23821 ttagggaagt ggggaactca gtagtattaa ctatttcaaa ggagtttctt aaagaaaatg
    23881 ggttaaatcc cggagatacg gtttttttag atgaagaaaa acttcgagcg gcaattacaa
    23941 aaaaacaagc cttatctgat gaaattgatt tagcaatatc tcaagcatta actgaatatg
    24001 atactacttt tagagagctt gtcgataaat gagtattcat tacctagaag ccaaagatat
    24061 tataaaaatg aatgtaatcc aaataaaaaa atactctcct aatgaacccc ttggtgtaaa
    24121 ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga gcctcagctt ttggtgaaga
    24181 ggtttatcct tcgatttatg aaaaagcagc tatcctactt atacaattga taaagaaaca
    24241 tccttttcac aatgcaaata aacgtactgc tttcttagct acttttgtct ttttgaaaat
    24301 caatggacag cttttagcta ttgatcaaaa agatgctgta aatttagcgg tatatattgc
    24361 tacgtatgat aaggatttcg atcaactaaa atatgaagtg atcaacacta taaagacaca
    24421 tacgcagccc atttaaggag ttactgtatg tgtctttata gggttattag ctgctatagc
    24481 gaaataattg aagtaaatta caaatagcta taattcaaat ccaactatat agaaaacgta
    24541 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
    24601 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
    24661 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
    24721 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
    24781 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
    24841 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
    24901 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
    24961 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
    25021 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
    25081 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
    25141 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
    25201 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
    25261 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
    25321 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
    25381 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
    25441 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
    25501 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
    25561 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
    25621 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
    25681 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
    25741 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
    25801 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
    25861 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
    25921 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
    25981 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
    26041 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
    26101 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
    26161 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
    26221 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
    26281 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
    26341 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
    26401 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
    26461 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
    26521 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
    26581 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
    26641 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
    26701 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
    26761 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
    26821 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
    26881 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
    26941 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
    27001 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
    27061 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
    27121 cccccactta gcttggatag gctacaatta actagacaga aaaattaagg tgtgtagact
    27181 agaagaaaac ataccaggag gaatttttat gtctaagaga acacgaagaa ctttttcaca
    27241 agaattcaag caacaaatcg tcaatcttta cttagctgga aagccacgtg tagaaatcat
    27301 tcgagaatat gaactaacgg cttcagcatt tgacaaatgg gtaaagcaat c
//
LOCUS       DAZKMZ010000031        26912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000031 DAZKMZ010000000
VERSION     DAZKMZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.031"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8M5_001423"
     CDS             complement(164..388)
                     /locus_tag="ACG8M5_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0358679.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8M5_001424"
     CDS             complement(586..2166)
                     /locus_tag="ACG8M5_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0358680.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8M5_001425"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8M5_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0358681.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8M5_001426"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8M5_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0358682.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8M5_001427"
     CDS             5331..5765
                     /locus_tag="ACG8M5_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358683.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8M5_001428"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8M5_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0358684.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8M5_001429"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8M5_001429"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0358685.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8M5_001430"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8M5_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0358686.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8M5_001431"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8M5_001431"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0358687.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8M5_001432"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8M5_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0358688.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8M5_001433"
     CDS             11001..11471
                     /locus_tag="ACG8M5_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0358689.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8M5_001434"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8M5_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0358690.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8M5_001435"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8M5_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0358691.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8M5_001436"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8M5_001436"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0358692.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8M5_001437"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8M5_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0358693.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8M5_001438"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8M5_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0358694.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8M5_001439"
     CDS             16164..16967
                     /locus_tag="ACG8M5_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0358695.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8M5_001440"
     CDS             17145..18092
                     /locus_tag="ACG8M5_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0358696.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8M5_001441"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8M5_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0358697.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8M5_001442"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8M5_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0358698.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8M5_001443"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8M5_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0358699.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8M5_001444"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8M5_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0358700.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8M5_001445"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8M5_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0358701.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8M5_001446"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8M5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0358702.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8M5_001447"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8M5_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0358703.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG8M5_001448"
     CDS             23883..25052
                     /locus_tag="ACG8M5_001448"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0358704.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..26911)
                     /locus_tag="ACG8M5_001449"
     CDS             complement(25319..26911)
                     /locus_tag="ACG8M5_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358705.1"
                     /translation="MFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSLESIFSS
                     PINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVM
                     YEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKE
                     SWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLL
                     LSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLIC
                     LIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTL
                     FISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQ
                     MENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGK
                     TIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFI
                     SIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         8233 a         5317 c         4285 g         9077 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa gtgattttag
    26041 ataagattcc taatttttta aagttaaatg ttagtataat taaacaaata agcaaagtta
    26101 agccaatacc aaatgatagt aattctgaat ctatacttcc aatcaaaaac tctttaccca
    26161 gataaattgc tcttttttgt aatatatcca cataaggtct attcagatct tgatacaatg
    26221 tctgctcaat cataggacct ataaacgcta gtgaaagaag taaagtaact atagcagctt
    26281 ttattaattt aataattaag aagatttcta ttttcttttt ccgataaaat tgaataatta
    26341 aagcaattat aatcattacc atagtcatag ctacagaaag aacatgagtg taaattaata
    26401 aagtcatgct aatcgccaac ataacccaag attctttttt tgagtcgtaa agctttataa
    26461 acccgagtaa aataagagga agtattgaca ttgcgagtaa ttcccccatt gcccctcgaa
    26521 gaaatatttc tacactccta aaagaagatg ttgtatatat aatagcgaaa aaggagctag
    26581 taacatgttt tttggtaatt tcatacatta cataatgaca tataaataga gtaatgagcg
    26641 taacaatata aagaaaaagg ttatagccta ttgctaaatt agtaaacttg atcaataaaa
    26701 ataaaggata tagagttaac catgggtaaa aattatttac ttgcatgcca tatgatctaa
    26761 acgtttcaaa attaataggg gagctaaaaa tagattctaa acttaacgca cgattaatgt
    26821 gaaatgccgt atcataaaag ccaaatattt ccatgttttt aaatgctgga aataaatata
    26881 ataaactaac aaatgcataa actataaata tt
//
LOCUS       DAZKMZ010000032        26811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000032 DAZKMZ010000000
VERSION     DAZKMZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.032"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8M5_001450"
     CDS             complement(104..1336)
                     /locus_tag="ACG8M5_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0358706.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8M5_001451"
     CDS             1591..2160
                     /locus_tag="ACG8M5_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0358707.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8M5_001452"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8M5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0358708.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8M5_001453"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8M5_001453"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0358709.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8M5_001454"
     CDS             3732..4655
                     /locus_tag="ACG8M5_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0358710.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8M5_001455"
     CDS             4731..5870
                     /locus_tag="ACG8M5_001455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0358711.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8M5_001456"
     CDS             5863..6663
                     /locus_tag="ACG8M5_001456"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0358712.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8M5_001457"
     CDS             6663..7490
                     /locus_tag="ACG8M5_001457"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0358713.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8M5_001458"
     CDS             7495..8202
                     /locus_tag="ACG8M5_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0358714.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M5_001459"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M5_001459"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0358715.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M5_001460"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M5_001460"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0358716.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M5_001461"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M5_001461"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0358717.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M5_001462"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M5_001462"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0358718.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8M5_001463"
     CDS             11787..13820
                     /locus_tag="ACG8M5_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358719.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8M5_001464"
     CDS             13864..15144
                     /locus_tag="ACG8M5_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0358720.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8M5_001465"
     CDS             15354..16160
                     /locus_tag="ACG8M5_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358721.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8M5_001466"
     CDS             16172..17392
                     /locus_tag="ACG8M5_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358722.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8M5_001467"
     CDS             17382..18968
                     /locus_tag="ACG8M5_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358723.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8M5_001468"
     CDS             19007..21145
                     /locus_tag="ACG8M5_001468"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0358724.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8M5_001469"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8M5_001469"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0358725.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8M5_001470"
     CDS             22874..24265
                     /locus_tag="ACG8M5_001470"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0358726.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8M5_001471"
     CDS             24281..25321
                     /locus_tag="ACG8M5_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0358727.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG8M5_001472"
     CDS             25340..26425
                     /locus_tag="ACG8M5_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0358728.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26811
                     /locus_tag="ACG8M5_001473"
     CDS             26458..>26811
                     /locus_tag="ACG8M5_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0358729.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEF"
BASE COUNT         8600 a         4451 c         5444 g         8316 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt t
//
LOCUS       DAZKMZ010000033        26658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000033 DAZKMZ010000000
VERSION     DAZKMZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.033"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1766)
                     /locus_tag="ACG8M5_001474"
     CDS             complement(66..1766)
                     /locus_tag="ACG8M5_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358730.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2273..3433
                     /locus_tag="ACG8M5_001475"
     CDS             2273..3433
                     /locus_tag="ACG8M5_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0358731.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3449..3892
                     /locus_tag="ACG8M5_001476"
     CDS             3449..3892
                     /locus_tag="ACG8M5_001476"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358732.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4375..4905)
                     /locus_tag="ACG8M5_001477"
     CDS             complement(4375..4905)
                     /locus_tag="ACG8M5_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0358733.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            5010..5561
                     /locus_tag="ACG8M5_001478"
     CDS             5010..5561
                     /locus_tag="ACG8M5_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0358734.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5714..6187)
                     /locus_tag="ACG8M5_001479"
     CDS             complement(5714..6187)
                     /locus_tag="ACG8M5_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0358735.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6325..7797
                     /locus_tag="ACG8M5_001480"
     CDS             6325..7797
                     /locus_tag="ACG8M5_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0358736.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7794..8504
                     /locus_tag="ACG8M5_001481"
     CDS             7794..8504
                     /locus_tag="ACG8M5_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0358737.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8856..9140
                     /locus_tag="ACG8M5_001482"
     CDS             8856..9140
                     /locus_tag="ACG8M5_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0358738.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9421..9840)
                     /locus_tag="ACG8M5_001483"
     CDS             complement(9421..9840)
                     /locus_tag="ACG8M5_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0358739.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(9842..10426)
                     /locus_tag="ACG8M5_001484"
     CDS             complement(9842..10426)
                     /locus_tag="ACG8M5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0358740.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10473..11948)
                     /locus_tag="ACG8M5_001485"
     CDS             complement(10473..11948)
                     /locus_tag="ACG8M5_001485"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0358741.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12129..12440
                     /locus_tag="ACG8M5_001486"
     CDS             12129..12440
                     /locus_tag="ACG8M5_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358742.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12555..14891)
                     /locus_tag="ACG8M5_001487"
     CDS             complement(12555..14891)
                     /locus_tag="ACG8M5_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0358743.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            15033..15701
                     /locus_tag="ACG8M5_001488"
     CDS             15033..15701
                     /locus_tag="ACG8M5_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0358744.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15704..16747
                     /locus_tag="ACG8M5_001489"
     CDS             15704..16747
                     /locus_tag="ACG8M5_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0358745.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16984..17865)
                     /locus_tag="ACG8M5_001490"
     CDS             complement(16984..17865)
                     /locus_tag="ACG8M5_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0358746.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            18024..18752
                     /locus_tag="ACG8M5_001491"
     CDS             18024..18752
                     /locus_tag="ACG8M5_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0358747.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18908..19225
                     /locus_tag="ACG8M5_001492"
     CDS             18908..19225
                     /locus_tag="ACG8M5_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0358748.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19218..19826
                     /locus_tag="ACG8M5_001493"
     CDS             19218..19826
                     /locus_tag="ACG8M5_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0358749.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19881..20672
                     /locus_tag="ACG8M5_001494"
     CDS             19881..20672
                     /locus_tag="ACG8M5_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0358750.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20810..21298
                     /locus_tag="ACG8M5_001495"
     CDS             20810..21298
                     /locus_tag="ACG8M5_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0358751.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21349..22221
                     /locus_tag="ACG8M5_001496"
     CDS             21349..22221
                     /locus_tag="ACG8M5_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0358752.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22397..22759
                     /locus_tag="ACG8M5_001497"
     CDS             22397..22759
                     /locus_tag="ACG8M5_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0358753.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22768..23520
                     /locus_tag="ACG8M5_001498"
     CDS             22768..23520
                     /locus_tag="ACG8M5_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358754.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23534..25336
                     /locus_tag="ACG8M5_001499"
     CDS             23534..25336
                     /locus_tag="ACG8M5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0358755.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25613..26653
                     /locus_tag="ACG8M5_001500"
     CDS             25613..26653
                     /locus_tag="ACG8M5_001500"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0358756.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         8493 a         4645 c         5397 g         8123 t
ORIGIN      
        1 taaaactctc caaacgatta taccgtttgg agagtttttt gatcggatgt atttatccct
       61 tttgcctaag cacgtaaaag cttgtatcta tgataaataa ataaacatag caaggtaaac
      121 cagccaatca aagtaagaga aagaatgaaa tagaaataga ttggcgtttg ataatgaagc
      181 gtgagctcgt tgattccttt ttgctgcttc acgattgggg tcccgatatc tgttacttca
      241 taatcagtca atgtttttcc attttgctgt agcacagtcc gatcgtagac gataacaggg
      301 atatgaacag ttttgtcccc agtgtttttc cacttgattg tcaaaagacc gcctggatgt
      361 gtttttataa aagttgtttc gttttctagt atttcttcgt tataccggtc gtatttattg
      421 tcttttgtct ccttatagat tggcagatag tcaggtgtgg ttttttggaa aacgaacaag
      481 ctggtagaca tatctttgct gaagaatgtt ttccttgctt cttccggcgt atcgaataga
      541 taagtatgtc gactgacaaa tgtttcattt tcccactttt ccagatgctg gtataagttc
      601 tggatcgtct gtgctgaaaa aataagaatg ccaaatatac ttaaaagacg gaaccacttt
      661 tgttcagacc actggttcag tgaaagggca aggtaaagta gaagcaatag cgtaaatggc
      721 acgaaaaatc gaaatggaaa ctggatcaaa ctaataaaag gcacattttt ttcgatcaat
      781 gtcgtccatg gaatcaagct agtagaaaga aaaaagaaaa caagacttgt gtacaacacg
      841 attttttgga ataaggtgtt cttttgttga aagaaaataa aaagcaaaaa acctgataat
      901 atcaatatag gaaaggcctt agggtaatgg agccaataac tgccatccag atctatcgcc
      961 ttttgactca tttctctgtt aacaaaggga ggaagaatct tattcccact gtaaacaata
     1021 gctaatcctg cccaaatatt tgctgtcaga acaaaataaa gcaagaccga cttaatcagt
     1081 tgaagcagtg cttttccttt ttcagtctgg ttaaaaaagt aataaagata aaaagggata
     1141 tacatcaaag ctaacatgat cgaagacagt aaatggatct gtgtcataag tcccactgag
     1201 acagcgactt tgataacagg gaacttttta tgaaggacga aatcagcaat agggatcata
     1261 cataaaggat aaaaacaagt cccccagcta gtgaatcctt ggttgataga ccagtattga
     1321 atagaatacg tactcatgaa taaaacggct actggtacac ttacccaatt tcgaacattg
     1381 acatatctca gtaaaatcag catagaaaat cctgaaataa gataaagaat gaaatttgaa
     1441 agaatctgat aatgaaacca gctacctgca agcaacacga tcaatccttg taaataagcg
     1501 aaaaatgggc cgtaaacggc attcacgatc ctgccacttt gctggaatcc ataaagggaa
     1561 ggaaaataat ggaaatcccc atatttaatt tgttgcgcgg catcatagaa ccgattgtaa
     1621 tggaatgggg catcagcacc caaaattagt ttatgtgtga ataaatgagg catgagaaat
     1681 gccaaagcaa tgaaaaaaat tgctaaaaat gggccatatt ttttcaattg tcgaactcct
     1741 ttgcaaactt tagataaatt catcatatat cctatgcgag aggtaggcaa gaaattagaa
     1801 aattcatgaa aatatttatg aattttacaa gaaaaacata agtgaaagtt ctaaggagag
     1861 aggatgcgac ataagttttg ttgcatctcc ttgttttgaa acagaatttt tcttaatact
     1921 cgaaacaaag tagctttttc atgacctctc ggtaaacggt gttcaatcag ctgcttctcg
     1981 gtaataagct cactagccta gaaaatatga gaagctattt tctgactagc gctcttatta
     2041 ctcaaagcat aacgcagttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
     2101 tgcggtaata agcccaaccc aacaaaaaat atgagtagct atttttttgg gctgtactta
     2161 ttacttgatg caggacagct ttttcacaac ctcatcggct ttcattttct atttttttcc
     2221 tgaacccata taataaaaga aagaacaaaa aaacttgcaa gggaggacac atgtgaagca
     2281 gagtaagcaa aaacagtctc gtcgactttt gtttcgtttg tttttattaa tatgtatcgt
     2341 cgtagggtta ctgatcgctt tatatccatt ttatgtggat tctttaaata gttttattga
     2401 tcaaaaacgt atcgaacaag tacaaaaacg gaccgcagct gaaattgaag tgcagcgaaa
     2461 gaaaatggag gagaaaaacc aacgattaac tgaccaagga ttcaatcctg gtactgatcc
     2521 tttcaatgaa aagaacagaa atgaaagtac aacatccagc caattagagg aatggctgat
     2581 cggaagtatc aatataccta agatccaatt aaatatttca ctttatgatc ggctcaatgg
     2641 taggatcctt gaaaatggag caggcgtctt gcaaggaacc tctttcccgc ttggtggaaa
     2701 ttcgacgcat tcagtgatct ctgctcacag cggtttacct aatcgacggt tatttaccga
     2761 attagatcgt ttagaaaacg gagacacgtt tattttgact gttttggggg agaaattggc
     2821 ttatcaagtt aaaaatatac aagtggtact gccagatgac acgtctgttc taacgatttc
     2881 agaaggagaa gacttagtta ccttgcttac atgtacgccg tatatgatca acacgcatcg
     2941 tttgctagtg acaggacatc ggattcctta taacgaatcg gtaaaaaaag aagaaggaaa
     3001 aggaaatcag gaaagaacca tgcggcaatg gtttattctt gcaggaacga taatagctgt
     3061 agtcatactg ttagtgttta tcggacgact aatctatcag tatagattaa gtaaaaaagt
     3121 gcttgatttt tcatttatta ttagtgattc gtcagggaaa ccggtaaatg gtagttcatt
     3181 cattttgaaa cataagaaaa aaacacttac tagaaatggt gtgccgtttt cagtacaaag
     3241 cgaccatcat gggaaagtca agctggatca attgcctggt ggaacttatc gaatcgtttc
     3301 agctgatgat cctaaagttg ctgtttcttt tggtattaga aaagtaaaac aagagaagat
     3361 atactttttt gaggggcgaa aacttgtaaa agagcttcaa aaaaatggtt tttattttaa
     3421 aatcaatgaa taagtaagga gagaaaaaat gatccgcaca acaacaaaat tgtctgccaa
     3481 agaactggaa tctgttctat cgatttggct gcatgctaac ttggattctc atgaattcat
     3541 tccatcaact tattgggaga atcaagtaga atctgttagg aaagctttac cagatgcagt
     3601 gatttactta gcagaaatag atcaagaaat tattggtttt gctggggtga ttgatcagtt
     3661 tatcgcagga atctttgtga aaagggaata ccggcaacaa agaatcggta cgcaactaat
     3721 gaaaaaaata aaagaaacca ataaaacact gactttgaca gtctatcaaa aaaatcaatc
     3781 agctattcat ttctatcttt cacaaggttt tacaattcaa acacagcaaa aagatgaagc
     3841 agtaaatgag gaggaacttg tgatgaaatg gaatcaaaac gctgattttt gatgaacaga
     3901 ataacgaaat aatgctccca gcctaatttt ttgctgggag cattatttca ttttgaagtc
     3961 actaatttaa ctgtgtgaat atgcagttgt tacaatcttt gataaacggt gttcaatcag
     4021 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt tccatttgtt
     4081 tctctaatac tcaggtcaaa acgctgattt cacaacctct tgataaacgg tgttcaaacg
     4141 gctgcttctc ggtaataagc ccactagcct agaaaatatg aaaagctatt ttctaactag
     4201 cgttcttatt actcaaagca taacgctgat ttcacaacct cttgataaac ggtgttcaat
     4261 cagctgaccc tcggtattag ctcaaaaaac ggcaaaacat gaggaactat ttttccgttt
     4321 ctttctctaa tactcaggtc agaacgctga tttcacaacc tctctttcac aaccttattt
     4381 tactcgttta taattttcat cgaagtattt tccgctacgg atatcatctg gcattccatc
     4441 gtatggttct tcgctaataa catcttcatt gtctatacct gcatcaccca tataagtgat
     4501 cgtagcaaat tcaggaacaa gtaattttgg ataagtttca tatttttcac gagctgcttc
     4561 catttctgcg atatgttcgt tttggaaagt aacagtgatt tctggatgtt cttcaaccca
     4621 tttagggaaa aagatcattc cgtgaagttt cttttcattt ggcataaagt ccaatgccac
     4681 gtctgtccct gttggctctg tccaagtgat tttgaatacg ccgtccgtca atttaacgat
     4741 atcagcttct tggtctttta cccaacggcc tttgaccatt ccgccatgga tacggtaatc
     4801 aacagtatgg tcatttttgg cataccattc atattcccaa ccattgtcat acgtatagat
     4861 aaagtgagtt ccaataaaat cttccaattc tttaaattct ttcatgttta agcatctcct
     4921 tataaattaa aacatgtaat aaactacatg ttttcgatta ctcatttatg atagaatgga
     4981 tttaaagatt tgtcaaggag ggaaaactga tggcaagaaa aaatacactt cgatatattt
     5041 tgttagggtt attaagtaag aagaagatga caggatatga attgaatcaa tcgttcaaaa
     5101 atgaaatagg agaattttgg caagcaaaac atagtcagat ttatcctgaa cttgcaaaaa
     5161 tggaagagca aggaatcatt cagcatcaag tagagatcac aggtgaaaaa ttggaaaaga
     5221 aagtttatga attaacagaa gaagggaagg aacaattggc agagtggatt catacaccca
     5281 ccaatgaact accggttaat cgagacgaat ttgttctgaa attgtatttc gtcaaagacg
     5341 tgaatgatcc ggcattagta gaaattattc agcagcagcg tgaccttcat gaagaaaaac
     5401 gacttcatct attagagcga aaaaaaacga tctttcctac tgaaaaagaa aaacatgaaa
     5461 attacggaca ctacctgatt ttagcgcatg cgatcaatcg ggaaacagaa tatgcaaatt
     5521 ggctaacaga tgtattagaa gaaagtaacc gaaaaaaata aaaaaatatc cgaacagtga
     5581 tggagaattc ctttctatat gaaagaaacg cagatcaccg ttcggatatt cttatgttca
     5641 aacaggcctt ttttcaagta tttatgtagc atctacttat taaaaagctt ggtacttagg
     5701 agattagtgg cgtttagagt tcttttaaca actgttcgta gtcagctttt tcatcgacag
     5761 gaaggagctt gtaagttaat tcgtctgtct cgttgtcatg aatgatgtac atcttagcat
     5821 tctcttcttt gaagttaccg atttgtaaaa tcgtatagcg ttcgtcgatc aattcgatat
     5881 ctttgattgc ttttgaaaaa actttacgga catggtgcat tttcttttgg cggatttctg
     5941 ccttttctgc agcttttgac aaagtaaatc cgtcatctaa atattttttg atcagttcta
     6001 cttttatgac tgtaggtaaa cgatattttc taggacaatt cgcatcagct tcgatcgaac
     6061 gaatgaatcc cttttgttcc caatacctta attgacgtgg agacacacct acgatatcac
     6121 ttaattcact gatgccaaca accaattgat cattttccaa caaacgttta agggaagccc
     6181 caaccatttt tgtcactcct tttgtcctct tgcttccctt aattatatcc gtattaactt
     6241 atgtgtcaag taggttgaca aatagcgaat ttaggactaa agtattctag tagtttcaga
     6301 aaaaattcag aaagaaggag aaatatgagt aacaaccaac ccgtagatat ttacggaaaa
     6361 ccatataatc ggacactttt agttgttgtc ttattaattg gtacattttg taccgttttg
     6421 aatcagacct tattgacgac agcttttcct acattgatga aagctttcga tatttcagcg
     6481 tctgatgtgc aatggttaac gactggtttc ttgctagtca acgggatcat gatcccaata
     6541 acagcatggc tgatcaataa attcagttca agaaatttat atctttctgc catgacgatc
     6601 tttttgatcg gaacgattac ttgctttaca gcacctaatt ttagtacatt actaatcggt
     6661 cgtttgatcc aagcttgcgg tgtgggagta tcgatgccat tattgcaaaa tatcatgcta
     6721 tctattttcc cgccagaaaa aagaggatca gcaatgggga tgtcagggat cgttattgga
     6781 cttgctcctg ctttaggccc tacattatct ggttatatca ttgataacta ccattggcgt
     6841 gacttgtttg gtatggtact gccaatcgtt gttcttgtct tggttttagc attcttccta
     6901 atgaaaagtg ttattccatt gtcaaatcct agcattgata ttttatccgc tattttatca
     6961 acgattggat tcggtagttt actttacgga ttttctagtg taggagatga cggctgggga
     7021 agcttgaaag taattggttt cttagttgtt ggtgtgattt tcatcgcctt gttctcatgg
     7081 cgtcaattac atttggaaca tccattcttg gaacttcgtg tattcaaatc atctacattc
     7141 acgattgcag ctgttttagc tggtgtaaca aatatggcga tggtaggggc agaaatggtc
     7201 gtaccattgt atattcaaaa tattcgtgga gaatcagcat tccattctgg cttgatgttg
     7261 ttgccgggtg cattgatcat gggggctatg atgcctgtga cgggtgcgat tttcgataaa
     7321 tacggcgcga aacgtttagc catcacaggg atgttcattt tgacagcggc aactgcacca
     7381 tttgctttct tgacaaaaac gactccagtt ttatatatcg tgatccttta tgcgatccgt
     7441 atgtttggga tctccatggt catgatgccg gtaacgactt ctggtatgaa tgcattgcca
     7501 aataacttga tcagtcatgg gacagcggta aataatacat tccgtcaaat cgcaagctcg
     7561 attggtacag ctattttgat cagtgtgttg acgaatgtga caaaagcaaa tatcccaagc
     7621 aaatctgtat tggataatac gccattagct tataaagatc aggcaatcaa tgccacactg
     7681 tcaggttacc atgccgcgtt tatcgtagcg atcatctttg ggattcttgg ctttattatt
     7741 gcctttttat tgaagaaaaa agaacctgtt ctaatgaaag cgggggataa tgcatgatta
     7801 ttggagtttt gattgtcagc ttgcttgctt ttgctttaac gaacgtgttc gctaaaaaaa
     7861 catggcaaac ctttttatct ttgattttcg gactaatctt cgtcgcttca ctaagtttga
     7921 tcgtcgctaa tttgtccaat cactttggaa tggaaaaagt aacggaaaca aaaacagaaa
     7981 aaattgtttc cagtgctgat tcccaaggag cagatatgct tttgtataaa gctttaggga
     8041 atggaaaaga aaaagtgtat ctttaccgaa caaatgaaaa acaagaaaaa ccaaaagcaa
     8101 ctggtacgga taacgaaaca aataaagtag aaaagacaga cggcgatgct gaaaaagtga
     8161 cgaaaactac ttattgggaa tacaaaaacg acatgtataa attctggttc aatattgctg
     8221 ataacaatca cgaatatgac agccgagtga atacattcaa aatcccagag acatgggtag
     8281 aattaagtac ggaccaagca gccaagttag ctgaattagt gaagaaacag caaagtacga
     8341 tggaaagtga agcaaaagct tatgttcaag acggtatggt aaaagcaatg actgaaaatc
     8401 caaaaatgag caaagcagag caagaacaac gtacgaaagc cttagctgct gaattccaac
     8461 aacaagcatt cgcaaaatta gtgaaagaag caaaaggcga ataatatttt gaacaccatg
     8521 taggaggttg tgaaaaagct gctctgcatc aagcagtaag aacaatcaga taaaaatagc
     8581 actccatatt tttcatctga ttgggcttag tgccgtagat gcaagctttt gaacaccgtt
     8641 taaggaagag gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa
     8701 cagcttctca tgtttgttga ctattgtgac ttgtgaccga ggagttggca ggagaacacc
     8761 gtttattcga aaaaaggaac tgcgacaaga cttttgtcac agttccttct tttttttgct
     8821 atgctatact agatgtaaaa aataggagaa ataaaatgag ggaaatttat tcttttgaat
     8881 caaaaatcta tgcttccgaa gtcggcaagg gcggggcata tattattttt ccgtatgata
     8941 taagaaaaga attcggtgct ggaagagtta aagtccaagc aacttttgac aatgtgccgt
     9001 atgaaggcag tatcgtgaat atgggcgtga aaaatccaga tggttcagtc tgctacatcc
     9061 ttggactacg aaaagatatt cgtaaaaaca tcggaaaaga aatcggtgat attgtagcag
     9121 ttacagtgaa gcaaaaataa aagaggttgt gacaaaagac gtgtcacaac ctcttgataa
     9181 acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaacc
     9241 catgtttaca aaatttttct tactactcga aacgaagcgg ctttatcaca acctcttgat
     9301 aaacggcgtt caatcagctg tccctcggta ttagctcaaa aacggcaaaa tatgagaagc
     9361 tatttttcca tttttttctc tcatactcag gtcataacgc tgatttcaca acctcttctt
     9421 tcataatggt ttttcaccat ctttataata aactggttct ttgatatgga aatctatttt
     9481 ttccaaccaa tctaaaatct tgtaacctaa ctcttctgac atcggaaaag aggcgttgat
     9541 ttgtgcattg gagatatggt caacatggat accggaagtg atcgtacaac ttcccggaac
     9601 ggcagttttg atgatcacag ctattttttc ggctaagaca tcgtctttat ggaaccgacc
     9661 atcgtggcta gggaaacgta cagtttgcat ctctgtatca cgagaaaatg tagtgactgt
     9721 tccgatatgt ggtgtatctc cgcctgttaa agtgatcaat aagtctttac cgattacttt
     9781 aacagcagct tggatcgtga aacctagttt ggttacttca actatcgttt ctttttccat
     9841 attaatcacc ctgccaacgt tttgaatgct catttacaat ttcaaattga tccaaaagtt
     9901 tcattgtatg gtgattaaca agatcttgga tcgtttgagg ctgattataa aaagcaggaa
     9961 ttggtggtat gatttgcaca cctagtcgtg ctaatttagt caagttttcc aagtgaatgg
    10021 catttaatgg cgtttcacga ggaacaatga ctaactttcg ttgctccttc attgttacat
    10081 cagcagcacg tgcaatcagg ttgtcgccta aaccaacaga gatacctgcg actgttttca
    10141 tacttgccgg tacgatgatc attccatcgg tcaaaaatga tccgctggcg acagcagcac
    10201 ctaaatcttt gtttgaataa tggtaatcaa acaggctttc gaattcagtt agtgataagt
    10261 cggattccag ctttaagttc tgttttgccc atggactgat gattccgtgt gtttctatca
    10321 tcggatattc ttttagtttc ttcactaggt tgatggcata aatggtgcct gaagcgccac
    10381 ttacaccaat cacgattttt ttcttccgtt gtgccaattg ttccattttc cgcactcctt
    10441 tttatgatgt caaaacagtt gcagatcatt cattatttca aatatttttc ccaatcttct
    10501 acttcttgga attttgcacg tacaaaatgt tctttcatat cgaaaggaac tgtaccgtca
    10561 aagatagttt ttgcacttat cccacggaaa cgaatagagc caggattata ttctggtaat
    10621 tctgatgggt ccaatggatg gttcctcatt cctgggatca cgatgatatc tttatctcct
    10681 tggaaacgag tgttgaaagt ccaaactaca tcgttcatgt cgaaaatatc aacatcttca
    10741 tcgactaaaa tgactgtctt caattctttg aaggctgata aagcaagaat agctgcttga
    10801 cgctgaatac cttcatccgc ttcgttttct ttacggattt gcatgatggt catcaattta
    10861 ccaccacctg caggaggatt gtagacattt actacttttc ctggaatagc tttatcgacc
    10921 aaggcaagaa tacttgcttc tgtaggaata ccggccatag atacgtgttc ttcactggga
    10981 ccaatcgttg tttgcatgat tggattgtct tttcggtgag taactgcttt gactttcact
    11041 acttgtacag ctggattagc atccccagta tagccaggaa attctggcat tgcttttcct
    11101 gagttcgtgt tgatatcttc ttgcattgtt tcatttggca tgatttctgc ttcaatgatg
    11161 aattcagcac gagcaatacc cacttcatcg actgctatac catcaacgat ttgaataggt
    11221 tcgttacgca aagctcctgc acaccatagt tcattgtagc caagtggagt ggtaggtggt
    11281 tcgaaagtcg tgccaatggc aattgctgga tctaaaccaa tattgatagt gataggcatt
    11341 ggtttattca aggcttcgaa ttgtttttgg aagtgaccaa tgtggcgtcc gccaggcatg
    11401 atgtacatac cgatcgtatc tttatcttct agaaccatac gatggatcgt tacatcgctc
    11461 attgtttttt ctggatctga tcctaagact acacccattg tgatgtatgg accagcatct
    11521 tcaggagtat ttgtaggtgc aggcaaaatg ttgcgaatat cgaagtctgc atcggtagct
    11581 agatgaacga cttcttgtgc tggagcgtct gctttgtcta ctttcacagg tttgattgga
    11641 ttttctacag cgtctttcaa gaaacgacca agtgttcgat aatcatggtg caaaatttca
    11701 cctacacgtt tacgactagc cattgttccg atgttcacac gtgtgccggg gaaacctttg
    11761 atattgttga aagtcatagc tggtccttct tgagtcggac gttttactgt accgccagca
    11821 ccgatataac ggtaaacacc agataattct gcttcaggat cgatttcaat atctgtttcg
    11881 tgatattgac ctggaagtgt ttttagttct tcgatgactt ttcttaaatc atacggttga
    11941 tctgtcatga gagattcctc cttagttgtc tttacctatc tatagtaaaa ggtgaaaaag
    12001 aggaaaacaa ttcaacttgt tcggtaaagt attctacaat ggaatggatg cgtttttttg
    12061 ataatagaat aaactatgat atacttatca ctaaaagaaa gcgcatactt agtgttggag
    12121 gaaggaaagt gaaaaaaatg gaggagtcat ttcctaaagc agtaaaagtg gaaaatatag
    12181 cgaatatttt gaaagttaca tttgaaaacg gagaagtaaa atacgtgaaa tctcattgga
    12241 cagaagagat aaccgatgcc ttgcaatttg gaaaaaaagg aagaggaaaa agaaaaaatc
    12301 ttctcgcact ttctcgtaat atgtggattg ggactgaagt aacgattgaa gcagatggaa
    12361 cagtattcat aaatggaaaa gacaggtata cgccagaaga gttgtggtac aaaggaaaga
    12421 aaagtatacc agaattataa aaaataatta aaaaaaacag cgcgaaacaa ggaacatatt
    12481 acttcctcgt ttcttgctgt tttctactat ttcagcgctc ttatgactca aatctaaatg
    12541 tggttgtcac aaccttattc tgcacgcaaa gcgatagcgg cgtctttttt cgctgccatt
    12601 cgtgcaggaa gatgaccgcc aagcatcgtc aatatagtag agacgatcac tagaataatc
    12661 gcatggacag gatttagttg agcaacattt tttagatccg tcatgttgta taagatgcta
    12721 ttgataggga acgtagctag ccatgcaata aacacaccta atatcccaga agaaatacct
    12781 aaaatacacg tttctgcgtc aaatacgcga gtgatgtctt ttttacgtgc acctaaagct
    12841 tttagtacac cgatttcttt tgtccgttca atcacagatg tataagtaat gatactgatc
    12901 atgatcatac ttgttactaa agagatccct gcaaaagcaa taaggacata agtgatagca
    12961 tccatcaatc cgccagtcag ttcagtcatc gttccagcta aatcagtata aatgatttta
    13021 tcttcatctg atttgccttt attgtaatca tctagataat ccaagatctt ttctttgtct
    13081 tcaaaattgt tcggataaat catgatactt gaaggcaagc tgtctccacc taaataacta
    13141 agcaatgttt gtttggcatt ttcgtcaact ttttcagtag ttaagacatt tacaccactg
    13201 tctctttgag ctttgacgat ttctgattct ttgttttcgt tgacgatttg agtcgttaat
    13261 tgatcgctat aagcgattcc tggagataag agattcattg tagaagaaga tttcacgcgt
    13321 aggatccccg agatcttcaa ttcatcagaa gcattctgat acatcgcatc ataatctgta
    13381 ttagggataa aattcccagt tggaagtttt gtatagtaag tattgttgtt tactaatttg
    13441 aatgttgtac caacgatatc atcaaaatca agtttttcgt cttctttcac atcaaatcca
    13501 agattcttca atgcgttgat attagtatta ttattgccgt ctacgatcag cacgacatct
    13561 gttgctgagg ctggataact tccggcaagt aaagaataat tgtcttttaa aaagttttct
    13621 ttgctagtgt caagctgtgt agggaaactt gacactccca cacctgtcat tgctgacatc
    13681 gtactagata aagaaagaga ttccgtatca ggattttgat tcgagaagct gacaggctgt
    13741 actttgccat ttacatcacg taaaagattg attcctgtag tacgggtaaa tccgatatta
    13801 ttacttaagt taggatcgat gtctgttacg taatcgacgt atgtttgatc aatcttattc
    13861 gtgtgctgtg ctttatcttc ttcgctgact tttgcagtaa tcgttttgct gtctggataa
    13921 tccgctttgc ttgcacccaa tccggcatcg tcacgagtct ggttagtcgt taccttagaa
    13981 atagtaatcg gaaatttcgc catcgtttca gcttgcgtgt tatcgatttg tttttggaat
    14041 ccagaagaaa gggacagaac gatcgcgatt ccgatgatcc caatactgga agcaaaggag
    14101 gtcaagaaag tacgtccttt ttttgttcgg atattgttga aggaaagctt taacgctgtc
    14161 caaaaactca tctttgttct gcgtaagtta aattgatcat cttttggtcg ttcgatgtga
    14221 ggatgggaat ctgttaagat cttaccatca gaaaattcaa tgattcgatc tgcgtattga
    14281 tgggccagtt caggattatg tgtgaccatg atcactaatt tttctttcga taactcttgg
    14341 atcaattcca tgatctggat acttgtttct gtatctaacg ctccggttgg ttcatcgcac
    14401 aaaaggatat ctgggtcatt ggctagtgct cgagcgatgg ccactcgctg catttgtcca
    14461 ccagataact gatttggctt tttatgcata tgatttgtca agccgactcg gtgcaatgca
    14521 tcttctgccc gttttcgttt ttcgtcttta gaaacaccac ttaatgtcat cccaagttca
    14581 acgttttcaa taatgcctag atgaccaatc aagttgtaac tttgaaaaat gaaaccgata
    14641 gagttgtttc ggtaggcatc ccagtcactg tccttaaagt ctttagtaga ctttccgttg
    14701 attaccatat caccggaatc ataattatct agtccaccaa tcacattcag catagtcgtt
    14761 ttaccagaac cactaggacc taaaatcgct acgaattctt tttgtctgaa agcgacagac
    14821 acaccatcca atgctttggt ggtggtttcg ccgactttat aatatttttt tatatctttt
    14881 aattgaagca taatcaccct tctcccgtat tgaaatattc ggtatactaa taagtatatc
    14941 agaacaatga tcgtagtata cgaaataaat gttaactgaa gatgaactac aagaaaaaat
    15001 ctagaaattt ttcaaaaaac agagggagat caatgaatcg tattttagta gtagaagatg
    15061 atccgcaatt acagttgctt taccgttctg tgttggaacg agtgggcttt tcagtcatca
    15121 ccgcattgaa tggagaggat gcgctgaaaa aactagaagc gacacgtata gaattaatca
    15181 ttacagatat catgatgccg aaaatggatg gctatgactt gctagactca ttacgtagca
    15241 ccagatttga cacacctgtt ctaatgatta cggcaaaggc cgattttgag gataagaaac
    15301 gcgggttcaa attaggagca gatgattata tgacaaaacc catcgatgtc aatgagatga
    15361 tcctacgtgt ggaagcattg cttcgacgtt caaagatcaa ttacagcagt agcttgataa
    15421 tcggacaaac aaaattagag ctagatgctt atacagtgtt acaagatcag cattcgttga
    15481 ttctgcctca aaaagaattt ctgcttctgt ataaattact gtcttatccc aacaagatct
    15541 tcactcgcca acagttgatg gatgatattt ggggattaga tacgaataca gaagaacgaa
    15601 cgatcgatgt acatatcaaa cgtttaagga cccgttttga aaagacggaa gattttcaaa
    15661 tcatcacagt acggggacta ggctataagg cggttattta aagatgaaaa aaaagctatt
    15721 ttcacaatta tggatctatt ttgctagtat tgtcttcatt tcgatcttag tgacactgct
    15781 gtgttttctc ggttttattt tcttattgtc tcgacattct atctcgcctg ctgaatcggc
    15841 tggaaaacca acgttatacc cgttattcgt ctttgcgggg ttcagtatgg tcgtgggtac
    15901 aggaatatcg atttttgttg ggcggcgtat cctgcatcca atcagtgcgc tagggacaaa
    15961 tatgagtctg gtagcaacgg gagatttttc gattcgaatg gatgaacagc agaaagtagc
    16021 agaagtacag caactgtata aagatttcaa cgttatggtc caagaactca atagtatcga
    16081 gacactgagg aatgactttg tgtccagtgt atcccatgaa ttcaaaacac cactagcaac
    16141 aatccaagga tatgttcaat tgttacaagc cccaaatctc tctgacgaag aacgccaaat
    16201 atttttatat cggattatcg aaagtatcac gcagctttca caattaacag agaacatctt
    16261 aaaattgaac aaactggaaa accagcgtat ccaactggaa aagaaagagt atcgattaga
    16321 tgaacaaatc agagaagtaa tcgttttttt tcagcctaaa tgggagaaag aacagctaga
    16381 attggatatc gaacttgctg cagtaaatta cacaggcaat gaagagtttt tgtaccaagt
    16441 gtggctgaat atcatggata acgccataaa gtacaatcaa atcaatggac agattcatat
    16501 caaactattt gagacagcca cagagattgt actggaagtg accgattcag gtgtaggaat
    16561 gaatgaagag accagagatc gaatgtttga aaaattttat caaggagata caagtcgtca
    16621 aatttctggg aatgggctag gactttcact ggttaaaaaa attctagaac tgcatgatgg
    16681 acgtattgat tatagcagta tcgaaggagt aggaacgaca gcaatgatac ggttaagtaa
    16741 acaataggaa aaagaataaa acccctaaaa attctattga aggtttttag gggtttttag
    16801 tcgttttttt gtgattaggg aaatgttctt acggttcttt tggcataaaa atcgctgctt
    16861 tcttattgaa ctacgagaaa tgttagaggt gtcattgaaa caaggacatg atgcaagaaa
    16921 gagagcgaaa gtcatgcgtc gctctctttt tttgggtgga tagatggaca gaaaatgtag
    16981 ggcttagttt tcttcttgat ttaaatactc ccaaaatgta tctgaagcca aagaatgctc
    17041 accgacttta cgagtaaatg caagataact ctctgttgtt ggagaaatag ggagaatcac
    17101 agtattttct tttaatagat tcttgactgt tttttccata acaatagcaa tccccatgtc
    17161 atttgcaatc atattgatga tcaagtcgat tcttgctcct ttataagaga tttttggttc
    17221 aaatccagct tcgcgacaca gatctagtac aggttgatac aagttcgtag tggggccaag
    17281 gagcaaaaag ttctcctttt tcagttcgct tagatccaat ttctttttac tagctaaagg
    17341 atgattttta ggtagaactg ctacaaaatc gtctgtttca gtaggcaacc actcaatcga
    17401 acttggtggg gcctggaaac ttctagcaaa gtggatcgtg tcactatgtt cattggcgtc
    17461 attgtaagtt aattcgacac tttccacttc tttcaactgg actgtaaatt caggatggcg
    17521 tttcaaaaac tcggtgattt tggaaaaacc tttataattg atcattgtcg gtatcgttaa
    17581 gatatttaat gaaagattgt tgtctttcgt ataggtttca agcacttctt gcatagcatg
    17641 gtattgttct acgacatttt ttacatatgg gagcaataag cttccagctt cggtcaattc
    17701 gatttttcta tgggcgcgtt cgaataattt gacgtctaat tctgtttcta acgaaagaat
    17761 ctgtttagaa atatttcctt gggtggtgaa ttgtcgatca gcagttttgg tgaaattcat
    17821 tgtttcggct aaatcaagaa aaacttcgaa tttatggata tccatcatat tcctcctact
    17881 tattccaata aagaatactt taacacaaaa aatgaattgg tgtaatcttc tctgtctgtt
    17941 acaatctttc ttgcagcgaa agcaataatt acagaaattt ttctgatttt tttcgcttcg
    18001 tagaaaaaga gaggagtttt ttgatgccaa ctggagtttt gatcaatgta ggttccgttt
    18061 tgtttggcgg actaattggc ggattagtag gaaataaatt aagtgaacat tttaaagcac
    18121 agctgacgat ggtttttggt gtgtgttcga tggggatggg aatctattcc attgcgccga
    18181 tgaaaaatat gccggctgtt atttttgctt tagtgatagg aacagcgatt ggtttgattg
    18241 ttcatttagg taatgggata aataaggggg ctgcgctgat gcaagtacct atttctaaaa
    18301 tcttccctag tgaaaagctt ggtatgaccc acgatgaatt tatcagcaca ttagtgacgg
    18361 tcattgttct attttgtgca agtggtacgg ggatctatgg aagtttagat tctgggatga
    18421 caggagacag tacgattttg atttctaaat ctgtccttga ttttttcaca gcagctattt
    18481 ttgcgtgtaa tttagggtat gtcgtttctg tgatagcaat tccccaattc attatttttt
    18541 atattttgtt cttattggca aaatttattt atccattgac tacaccagac atgatcttag
    18601 attttaaagc ttgcggcgga ttcttgatgg tcgctaccgg ttttagaatg atcaacgtga
    18661 aaatgttccc agtagctgac atgatacctg caatgatcgt tatcatgcca ctgagctgga
    18721 tgtggacaaa ttggattatg ccactgttat aataaaaatc aaagagaagc cattttttgc
    18781 tgactaaaaa ggatcaaatc ttttagtcag tttttttgta tttgacaaac tgattttgaa
    18841 actatattat atgttgtata atatagattg aaataaaata ttacacttga gaatagaagg
    18901 tgacgtaatg cacatccaga ttccaactgt tcttcttgat ggtactgtgc tagcagcatt
    18961 aagcaaggaa gatatgtatg ggtatgcctt aacaaaaatc gtacaagaaa gtctctctgt
    19021 cagtgaatct acactatatc cggttttgag aagattgaaa aaagaaggat atcttgagac
    19081 ctatgatgaa ccttttgaag gcagaaaccg tcgatattac tcgttgacac caatgggaag
    19141 tcgtcattta gaagaaatta ttgatgaatg gattgttttt cgagattctt tagaccggtt
    19201 attggaggag agcacaaatg aatgaatatt tgaatgaagt tgtggatcat cttcaagaaa
    19261 ttcctgaaga cgatcgtttt gatttgatcc agtattatga ggaatatttt ttagattctg
    19321 gaaaaacatt agatcagatc attgatgaat atggtacacc aaagcagttt gccttaaaat
    19381 taaaaatcag ttacttttca gaagaagaac aagcagaaaa cgagttagtg aaaaatccaa
    19441 aaagacaaat tcgcctgatt tggttaatca ttatcggttt atttgcttct cctgtcctca
    19501 ttccactagc actggggttt atattggtta ttgggggact attggttgct ttactagcgc
    19561 tcatttttag tatctatgtt ttatgtatca gtatcttagg tgttggccta ttcatgatca
    19621 tctcaggaat cagtgtgttt ggacaatcga ttctgagtgg attattcttt atcggtgtcg
    19681 gtctgttcgc cacaggaagc gcagtattta tttcgccact gttgctgaaa ggaacaaaag
    19741 ggctttttta tgtcatgatg aactttgtta aatggatggg acgacgtttt attacaaaaa
    19801 gagatctgca ttcagcgggc gtttgaagga ggacacataa attgagattg aaacattttt
    19861 tgattaccgg tattggtctg atgcttactg gtggcctcct agcaggtgca acttatacat
    19921 taggcgctca aaagagtctg acttgggata atggtcctcg gttgatcgat atggttactg
    19981 agacaaaaaa aatcaaggat gcaaacaaaa tcgtcatcca tgcagaaaat caaacagttt
    20041 cggtcatacg cggattggat tttgagataa aaacgactta tgataagaca gaaaagccag
    20101 aaataaagga gaaaaatggc gtgttgactg tgaatgcagt caaaaaatca acaggtgcaa
    20161 tcattagttt agaagacaca agtgcacaaa tcactattgt tgtccctgca gatcatgaat
    20221 tatctgaatt gacaatcgaa gggaataata gtgcaattga tatcaacgaa gtatcaatca
    20281 aaaaagtgaa tactaagaca catgatggct gggtatcgtt caatgaattt acaggaggaa
    20341 caataactgc gaaagatgaa ttaggctcac ttcgattaga tgaggccaat gtggataact
    20401 tgatcgtcac aggtgaaaac agcaacttcc atctagaaga cagtttgatc agcaaagaag
    20461 gaaaagtcat cctaaacggt ggcaatttat ctttgcatga gatggaaaac agtggctata
    20521 ctatccaaac gtctggtgaa tcttatattg aaaaagacta ccagccaata acaaatactt
    20581 tgttagaaca aggacagaaa aagctaaaga tcactgctaa agacacatcg atcaatatta
    20641 cgacggatca agaggacaat gaaacagatt gatcctgtac tcaactggat attttaagaa
    20701 aaaacagcaa agatccttcc aatctttgct gtttttcatc gcttaacgca cataactata
    20761 gtaaaataag aaagaaaata tctaaaaaga aaacagggtg aaagagtata tgaaggtaga
    20821 agaaggtcaa agagaaaagt taaaaacaga aatggaaaga ttacatactt atatcactca
    20881 actaagccaa accttctatg atccagacaa agaaaaagtg atggttaatt acccgaacaa
    20941 ttcagaaggt cgccaattag agcaagtgta ccatgaagtt ttcaaacatt tattgacggt
    21001 gaagaaagag ctggattatt attctttacc gattatcgat acaggaatat tgaagtatga
    21061 tgggaagaag gaacgattta tctttaaatc tgttcgggaa gatttgcctc tttcagcagg
    21121 gatggatcta gaggtcttgg tagaagacta ttttacagaa gaaaagcact gggttcgaac
    21181 aaaattggat tatttaccgc aggcagccgg agggacacag gcaagcggtt ggtacatcac
    21241 tgaagacaaa gagttagaac tagaaggtgt aatggcgcgt attcgaaaaa aaaattaaaa
    21301 aagtaacatt agatatttat tgaaggataa aggagcgcag gaagtagcat gagagaaaaa
    21361 agagcatttt atatcaatgg ttatgcaggg attgtcggat tggtcgtatt agctttagtt
    21421 ggattgtttt tcttctattt aggtatgtgg caagcgaaag tttgggcact ttttttaagt
    21481 atttttcttt ggttgatcac gttattgttg ttaagttcgg caactgtagt cagtcctaat
    21541 caagcaaaag taattttgtt ttttggacaa tatttaggta cgattcgaga aaatggcttt
    21601 ttccttacga tacctttggc acaaaaaatg actgtttcgt tgaaagttcg gaatttcaat
    21661 agttctgttt taaaagtcaa tgatttggat ggaaacccca tcgaaatctc agcagtagta
    21721 gtctttaaag tcgttgatac agcaaaggca ttattcgatg tagcgtatta tcaagatttt
    21781 gtagaaatcc aaagtgagac agccattcgg catatcgcta gccaataccc gtatgataca
    21841 ttcaatgaga atgatttgac gcttcgagga aacacgacgg cagtgtctga cgagttgcaa
    21901 aaagagctgc aagagcgttt agcagtagca ggtgtcgaag taatcgaaac acgcttgaac
    21961 catttagcct atgcaacaga aatcgctagt gccatgctcc aacgtcaaca agcaaaagcc
    22021 attttgtctg ctcgacaaac cattgttgaa ggagctgtca caatcactca aatggcctta
    22081 gagcaaatcg aagatggaca agaaatcaat ttcaccgatg atcggaaagt ccaattgatc
    22141 aataatttac ttgtctcaat cattacagac aaaggaacgc aacctgttat caatacaggc
    22201 gacatgaaag accgtaagtg acatactttt tgtaagatag gaagaaggag tttaggacgg
    22261 attattgtct tgaactcttt ttttagttgt tttaaagaaa atgacaaaat tgaaagaaga
    22321 atgatagttg attcgaaggc agaattgttc gcttttttct ataatcagta tataaagtaa
    22381 gagagaaggg gaaaaagtga aaaaacttct gatcggtcta cttgttttca tcgttgtggt
    22441 acttgcaggg gtgaaaatcg cagattacgt agttatgggt ggagaagaat actatgtaca
    22501 aattacgaca gatggtacaa aaacagtttc aaaatcagac tctggacaag aaacagtcaa
    22561 ctatagatat atcctgcctg gctatacgaa agagggtaag gaaaaagaac tggacttcaa
    22621 gggacaattg actcgaccgc ttcgtaaagg agcatattta aaaatcacct ggaacaaaaa
    22681 taaaggtgtc acttcgtatg aagaagtcga aaaaaaagac gtgccgaaaa cagcactgga
    22741 aaaactaatt aaagggtagg gaaagaaatg aacaaaatag tcgaagtaca gaatgtaaca
    22801 aaagtatacg gaaaaaacaa tgagaaaaag acgcaagcat taagcggcat cagcttcgat
    22861 gtagaaaaag gcgaatttat tgggatcatg ggcgcttctg gttctggtaa gtcgacactt
    22921 ttgaatattt tatccacatt agataaacca acagatgggc atatccgaat caatcaaaaa
    22981 gatgtgacca cattaaaagg aaatcaacta gcagattttc gtgcgaatga gattggcttt
    23041 attttccaag atttcaattt attagaaagc ttgacagcac aagaaaatat cgctgttcct
    23101 ctttctttac aaggcgtcag accaaaagaa atcaagcaac gtgtccaaaa aattgcagaa
    23161 cgtttatcca tctctcatat tttggaaagc tatccttctg agatttccgg tggacaaaaa
    23221 caacgggtcg ccgccgcacg tgcattgatc acacaaccaa cgattctatt aggggatgag
    23281 cctacaggtg cgctggattc caaaagtgcc cgggatcttt tagatacgat ggacgaattg
    23341 aatacaaaag atcatgtatc gattcttctt gtgacgcatg atccgttttc tgcaagttat
    23401 tgccaacgta ttttattcat caaggatggc ggtatccatc aagaagtgaa acgtggagat
    23461 caaagccgtg attccttcta tcgagaaata ctatctattc ttggaaactt ggaacagtag
    23521 ggggagattg actatgcttt ggaaattatc acttactggg atcaagagcc gtttaagaga
    23581 ctacttggtg ttattctcag gattagttat ggcatctgct attttttaca tgtttgaatc
    23641 aatggcaagc aacgaagctt ttttgaaaag taatacgatc gttgcggcta cagttatcat
    23701 tttccgattc ggttctgttt tactagggat catcacattc gtttatattt tatatgcaaa
    23761 ttcctttttg atgacgatgc ggcaaaaaga ctatgcaatg tttatgatgc tgggagcaaa
    23821 aggcagaaaa atcgcacaga tgattttttc tgaaacattc ttagtaggga tcgcagctac
    23881 tttagtgggt tcagctgtcg gtgtaggatt gacaagtatc gtccaccgat tattagtgga
    23941 tcagttagat ctcaatattt cgcattttac accttttagt attcacgggt tgttgatcac
    24001 tgttttgttt tttgctgttt tgtttttatt agctgctgtt gtcaatgctt tttcgattgt
    24061 caaaaaaccg attttagcat tgattcgtgc ggatcagaca ccaacaagaa tcaagcagca
    24121 taaattcttg tttttccttg aagtagtact gggaattgct tttctaggct gcggttacta
    24181 tatgatgaaa cacgtgggga cctatcaagt ctttggtata ggggtagcct tagttacgat
    24241 cgtattagga acttatttca ttttccattc tgtgatcatt ttctttttat cattactaaa
    24301 gaaatcagaa cagatttcat tgaaaaaatt gaacaatttc acattgtcac agttaaactt
    24361 tcgtattcgt gaatatacac aaatgctctc aatggtcgct atgctgtttg ccttggcact
    24421 gggcgctttg acggtaggat taggttttaa aaatgaaatt gcagacttga cagaaaacaa
    24481 ttctggatat gatctagtga tgaataatcc tcgggcagaa gatcagcaaa aaatcaaaga
    24541 actttcacca acactttctg cttcttatac tcaaaaagaa gatgcgggaa cgatttacta
    24601 taatgcaagt gaatttgcgg ctcaaccatt gatcatgata aaacatgaag atggaacacc
    24661 gccgaagatc acgaaagaaa aagttcctgc taaaaaaatg aatgaactga ctgtacaaga
    24721 ggaacttcga agctatgaat tgccggaaca gcaagaaaaa gaaatcaagg tagtcagtca
    24781 atcagaattt gatcaactga acttgccaca atccacttta cagctagttc aggtaaaaga
    24841 cttccaagca aacctgaaac caatcgaaac acttgttgct caaaataaaa tgaacaatcc
    24901 atctttgcaa aaagtagagt acagcaacca gaaatatgaa atgtatgaag tgtataatgg
    24961 tctattctct ggttttgaat ttatgggatt cttcctaggt ttagctttct tgaccatgct
    25021 tgctagctgt ttgatgttca agatcttgtc aggaagcaag agtgacattg ctcgttatga
    25081 aatgcttgaa aagataggaa cacgacgcgg gttgcttaaa caatcgatcc gtagagaagt
    25141 aggcgtgtta ttcttagcac caggaatctt aggtgtagtc catgtcttat tcggattaca
    25201 aatgtttggt ctattcatgc aaaatccata caaagatatt tgggttccgt ttacgatatt
    25261 cttcgtattg tatttcatct actatgtact gacgacatgg ttatatactg gaatcgtatt
    25321 gaaaaagaga ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aagcaaaaat
    25381 agctcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
    25441 gtttgttcgg aaaaagaggt tgtgacaata tttttgtcac aacctttttt tcacggtctt
    25501 ttattagaat atagtaaatt ttttctcgtt actcacggaa taagctgtta agaaaacggt
    25561 ttccatgtat actggaatca gtgcaaatga aagaaaagag ggtgcaagga acatggaaat
    25621 caatgaacaa aattttggcg aaaactatca cttgattacg atcacgaatc aaaatgggac
    25681 atctttgtct gtcagtgatc ttggggcacg ggttgttcat tttcaaacaa taatcgataa
    25741 tgaaaaacga gagttgatct taggttttga tgaagcaaaa gaatatctag aaaaagaccc
    25801 ttatatcggt gcttcaatcg gacgtatggc tggacggatt acgaatggca cattcaaatt
    25861 agatgatgaa acgttcgaag taaaaacaga cccggacact ggtcattgtt tacatggtgg
    25921 tgcacctagc tttgaagcca agcagtggca ttatacagtc atcaatggtg aaaacgaagc
    25981 atcggtcatt ttctatcaga ctagcccaga tatggaaaac ggatttcctg gaactttaga
    26041 tgtagaagta cggtatacgc tgacgaacga tgatacatgg cgagtgacgg tccaagggat
    26101 cagtgaccgc aaaacagtat tcaatcctac aaatcatgtc tatttcaatt taaccggtga
    26161 tccagcacag tcaatcgacg aacatatact ttggttgaat agccgattat ttgctacgct
    26221 caataaagat acaacgccta caggagaagt agtttcagta agtggcacag catttgactt
    26281 ccaacgtcct aaaaaattag gtgaggtctt tgccagtgat tttcgccaat caatgatagt
    26341 gggcggcatc gatcatccat ttttgttgaa aaaacaatct ggtttactaa atgctgcgga
    26401 attgacaagc ccagatgaaa aagtatcgat ccaagtaaaa acagatgctt caggaatcgt
    26461 gatttttaca gcaaattttg gagaagatgg accggaaatg cgcgggaata aattagtaaa
    26521 tcatggcggt atcacatttg agactcaaga actgccagga gcggagcgac ttgaaagttt
    26581 tggagatgtc actctggaac ctaaccaagt acgcgagttt gtaacggaat acaaaataaa
    26641 tataaagaaa tagaataa
//
LOCUS       DAZKMZ010000034        24981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000034 DAZKMZ010000000
VERSION     DAZKMZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.034"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2193)
                     /locus_tag="ACG8M5_001501"
     CDS             complement(49..2193)
                     /locus_tag="ACG8M5_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0358757.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2550..3923)
                     /locus_tag="ACG8M5_001502"
     CDS             complement(2550..3923)
                     /locus_tag="ACG8M5_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0358758.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3923..4318)
                     /gene="rbsD"
                     /locus_tag="ACG8M5_001503"
     CDS             complement(3923..4318)
                     /gene="rbsD"
                     /locus_tag="ACG8M5_001503"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0358759.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4346..5263)
                     /gene="rbsK"
                     /locus_tag="ACG8M5_001504"
     CDS             complement(4346..5263)
                     /gene="rbsK"
                     /locus_tag="ACG8M5_001504"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0358760.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5371..6384)
                     /locus_tag="ACG8M5_001505"
     CDS             complement(5371..6384)
                     /locus_tag="ACG8M5_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0358761.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6465..7064)
                     /locus_tag="ACG8M5_001506"
     CDS             complement(6465..7064)
                     /locus_tag="ACG8M5_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0358762.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7250..7951
                     /locus_tag="ACG8M5_001507"
     CDS             7250..7951
                     /locus_tag="ACG8M5_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358763.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7961..10672
                     /locus_tag="ACG8M5_001508"
     CDS             7961..10672
                     /locus_tag="ACG8M5_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0358764.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11123..12751
                     /gene="treC"
                     /locus_tag="ACG8M5_001509"
     CDS             11123..12751
                     /gene="treC"
                     /locus_tag="ACG8M5_001509"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0358765.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12811..13992)
                     /locus_tag="ACG8M5_001510"
     CDS             complement(12811..13992)
                     /locus_tag="ACG8M5_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0358766.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14244..15767
                     /locus_tag="ACG8M5_001511"
     CDS             14244..15767
                     /locus_tag="ACG8M5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0358767.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15836..16564)
                     /locus_tag="ACG8M5_001512"
     CDS             complement(15836..16564)
                     /locus_tag="ACG8M5_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0358768.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16577..17857)
                     /locus_tag="ACG8M5_001513"
     CDS             complement(16577..17857)
                     /locus_tag="ACG8M5_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0358769.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17998..18600)
                     /locus_tag="ACG8M5_001514"
     CDS             complement(17998..18600)
                     /locus_tag="ACG8M5_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0358770.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18845..19051
                     /locus_tag="ACG8M5_001515"
     CDS             18845..19051
                     /locus_tag="ACG8M5_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358771.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19363..20082
                     /locus_tag="ACG8M5_001516"
     CDS             19363..20082
                     /locus_tag="ACG8M5_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0358772.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20163..20846)
                     /locus_tag="ACG8M5_001517"
     CDS             complement(20163..20846)
                     /locus_tag="ACG8M5_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0358773.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21064..23250
                     /locus_tag="ACG8M5_001518"
     CDS             21064..23250
                     /locus_tag="ACG8M5_001518"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0358774.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23596..24006
                     /locus_tag="ACG8M5_001519"
     CDS             23596..24006
                     /locus_tag="ACG8M5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0358775.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24020..24376
                     /locus_tag="ACG8M5_001520"
     CDS             24020..24376
                     /locus_tag="ACG8M5_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0358776.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8372 a         4437 c         4872 g         7300 t
ORIGIN      
        1 aaattaaaaa gggagtgtga cagtgtcaca tccccttttt aatttattct atttctttaa
       61 aggcagatca agccagaatg atttgtctaa acttaacaag gttagtacag gaacagttgc
      121 agctttaact gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa
      181 atcccagatt gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga
      241 aaattctaat cctgtgattg gcacgccatc tgttgtccat ttgattggca catcgaatcg
      301 cttagacata ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt cttttttcgg
      361 ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt gtgaatattg
      421 gcgatctgta gcataacgtc cttccaaata tttggttgct tctttgtagt cttttgtttc
      481 cgacttccaa gttggtttgt agaaatcagg attgccagtc agtccatttt tgatcaagtc
      541 ggcatagtct tccaaacttt ccttgtaaga aggatattta cggaattgtg cgcggatttg
      601 gtagctttgt ccggaactat cttgttcaag tgtattgaag gtcacgctgc ttcctttata
      661 tgatcctttg ataccaaaaa ggttatgatt tggttcgctt gataataagc tgtttccaga
      721 tccactttca aggatagctt gagccatcat gactgatgcg tagattcctt ctttttgccc
      781 gatttcctgt gcatcatctg caatcttttt caagaactga tcagttgttt ggttttgact
      841 gaaatggaac gagccatcta atccatctaa aatagatgag ccgcctgtca gcgaacctga
      901 agcagcaggt aaacctgcta agtttactcg gacagcaaaa tcaggcgtga aatctgaaat
      961 agaagttact cgaactttat ctcctggttg aggcgcatgg ataaattgtc cgttcccaat
     1021 atagatacct acatggtgag taacaccgga agaaggtccc cagaagacga ggtcgccagg
     1081 ttgggcctga cttactggaa tctgcgtacc tgcatattgc tgttcgcctg tccagccacc
     1141 gatataatga ccggttgctt tcatatatac gtaataagtc aaaccagaac agtcaaaagt
     1201 atttggacct tttgctcccc atacatatgg ttttccaaga tgcttgaatg cttctcgaac
     1261 gattgcttgt tggacattcg ttgtatttgt aggcaataca gcatcaattg gtgattttac
     1321 aacaaaatca gaagaagcag aagatggatt ctctataccg ctttgatttg ctgtatttgg
     1381 acgagatgaa gcattgttcg ttccgccatt cgatgaacca ttgtttggat ggtttggttc
     1441 tctctgagta tttccattgt tattcgatcc agatgggttt gtttgattcg aagaattccc
     1501 gctgttcgaa ttattgttat tattgtttga attatttgaa ccattattat tgttgttatt
     1561 acccacatct ggtttgtttg gcgtttccgg atctggtgta ctagggactt cttcactagg
     1621 atcagttggt ttctctgttg aagtatccgg tccagcttta aattctgcag taatagataa
     1681 attgttcgta cctactgtaa aggtctttgc atcataattt acttctttgg caccattaac
     1741 agcgactgaa gcaatttgtt caccttcagc tggcgtaata gtgtaagtta ccttcgtttc
     1801 cgctaataaa ccttctaccg ttccactttc cgtaatatcc aaaggttgct gttcagggga
     1861 atccgctgtc gaagcagtca gttggatctt accaatcaaa gaaggagcaa ttgtaaggga
     1921 atatgtctcg gctggctttt cattttctga tggatcagcc ggatcaattt cagttggctc
     1981 ggtcgatcct gttgttgagt tagttgttcc agttgggtct tctgtaaaag aatccgttgt
     2041 ttcactagta gctgagctgg atgtagtcgt atctgttgtg actgtttgat cagctatttg
     2101 attatctacg gcaaatacgg tattggtcga caaaacagac ggagcaatca gccaagtagc
     2161 agctagcaaa cgtatcgttt ttttattttt cataataaac tagtcctttt cctttcattg
     2221 attttctgtc ttattcagca ttcattagac attttttgga acagataatc gtgtcgaaaa
     2281 gctttttctt ttcataaatc tgtctgtgta ttctaaataa aacaatttgt aaataagcaa
     2341 cgagcattca ttcgtttttc tttgacgttc attttattgc atagaccacc tttgtttggg
     2401 gataaacaag tggggataaa aatggttatg catatgtaaa ttataccaaa gtttggaaag
     2461 tatcacaaaa atttataaaa ataatgatcc taaaaagaaa aagactgaaa tatgtgataa
     2521 acatctcata cttcagtctt tttcattaac tattcataag acattaggtc ttcatcaaac
     2581 ccgctaacga gagtagtgat cattgctacg actagactaa cagccagtcc tgctaagtac
     2641 cagaaaaaag taccggaaat gaatgcaggc aatgtggtca gactaccaaa tacaaaagcg
     2701 atcgctttca cttgggcaaa tcccattaca gctccaccag cagctgcacc aatgcaagca
     2761 gcaacaaatg gttttttcaa tgggatcgca attccgtaaa cagttggttc ggtcactccc
     2821 gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac gggtttttga acgtttactt
     2881 cgcagaaata cacctaaagc tgcaccagcc attccaaaat tggctgctcc tgtcaacgga
     2941 acgattcggt cgaacccata agcagagata ttactgatca tgacagggtt tacagcccat
     3001 tggataccaa atattaccaa aatattccaa ccaccagcga tgattgcgcc actgatcggt
     3061 ccgttaatat ccatcatcca agtaattgcg gcagctaaac cttcgccaag gtaaacaccc
     3121 gcaggcccga ataccattaa cgtcaaagga accgtgataa ctaatgaaag taaaggtaca
     3181 aatactaatt gcatattttc gcgaatatat tttttgagga atttttcaac aaaagaataa
     3241 aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat actgcatcag tactacagga
     3301 atccccagaa aatcagtagc tgctttgcca ctgtctgaaa gtaaagaggt cagattcggt
     3361 tcaatcagtg ctgcagcaat gacagccgca atatatccat tcactttaaa cgttttggca
     3421 gcagaaaaag caagaaagat tggcagaaaa taaaagacgg cattcgacgc agcagacaaa
     3481 atatgataag ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa aacaaccaga
     3541 ccttttaata taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat ttccgatatc
     3601 aaaccaaata gtttttcgat cggatttagc ttttctttcg gagctgaatg atcgatttga
     3661 cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca tgattatttc tgcatatcta
     3721 tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt ttaatttttc tgcattggct
     3781 actggctgtt ttttcaattt gatacgaagc cgagtcgcac atggaaggac agaatcaata
     3841 ttgtcttttc ctcccaactg ttcaatcagc atatgaatct gctcggacag taccatctgt
     3901 tcaggattca ttgtcgtttc cattagaaga ccactccact cactaatata atattggcgt
     3961 aaggtgtcat ttcgcctgta cgaataaatg catgacagtt attcaactct tttttcattt
     4021 cttcatgtgg gataaagcta atcggtgtat ctggtaacaa taatttgatt ttttccaata
     4081 tttccgggtt cattgtcttt atttcttccg ccaattcaat cgattgtacc tcaagttctt
     4141 ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc gtttgagaca gccaaatcta
     4201 ttttttcagt accaaagggg acaggcatgc ctgcatcccc gatacttaat ttatcaaagt
     4261 gccccatttg tgcgattact cgtgaaatat ctgaattgat tacttttcct tttttcatgt
     4321 ttgtttcttc ctctcattgt taattttaga ctgctgattg ttctaaagct tcgcggtaag
     4381 ggatagaagg ttgcgcacca taagactgca cagcaacaga agaggctaga tttccataaa
     4441 ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat gcgccaataa
     4501 atgtatcacc tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg acgataccgt
     4561 gttctttttc tgtatgatag aaagcgccgg cactgcctag tgtaatgatt actgttccaa
     4621 tacctaattc atggagtttt tcggctgctg cgaccaaact gttatgatct gtcactctga
     4681 ttcctgtaat gatttctgtt tctgtttcat ttggtataat gatatctgtc ttttcaagta
     4741 gttcagtagg aatcgtttca cgagctggtg caggattcag gatcgttgtt tttcctgcgt
     4801 cttttgcaat agaaaaagct aggatcgtcg catctaagct actttcaaac tgagcaataa
     4861 taaagtcact gtttgtaatc agttgcttgt tttttaggac ttcttgttca tggaaagcat
     4921 tatttgcccc tgcatggatc aagatactat tttctcctgc tgcgtctacc ataatcattg
     4981 cttgtccggt tgtggcgcct tggatttcag ctatcccaga tgtatcgatg ttttcttttg
     5041 tcagtagatc taataactgt tgtccttcgc tgtcagcccc aacgcctcca ataaaagatg
     5101 tccgtgcgcc tgaacgtttg gctgccactg cttggttggc acctttacct ccgccagcgt
     5161 gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg catgttggtt aaacgaaggg
     5221 tagtgtccat attaatactt ccaattactg taaccgtgtt cattttacaa acctccaact
     5281 atctcattta ttttgatggg aaaattattt tttaaaacgt tttagtaaaa cgttttaata
     5341 gttcgataaa aatatagcac accttgaaag ctatttcaag ggtgcagttg aaaatctttt
     5401 ttctaatcgt acaggtaatc ttttttcttc ccattctttt tctgggaatt ggatccgatc
     5461 taacaataac ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga tcgttgtcag
     5521 tttcggcttg atatactcgc acatatctat attatcatag ccaataatgc tgtagtcttc
     5581 agggatgctt tttccagctt cctctaatcc gcgatataat ccaaaagcta attcatcatt
     5641 taaagcaaac acaccagttg cagcagaatc aagaagttct gctgtgatct ggtagccacc
     5701 ttgttttgag aactgtgtag gtaagaggat cagttggtcg tgagggattt ggtaaacatc
     5761 aagagcagat ttaaacccct caatccgagc gtggacattt tccggtggat tttcaggata
     5821 gacaagggcg atcgtttgat gtcctagact aagcaagtgc atgcccgcta ggtaaccacc
     5881 gcgaaaatcg tctgttcgta cggcatcact aaatccttca gattttttct gatccaaaac
     5941 aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag agacagcaga
     6001 agtcgcgata atgaatccat ccacacctcg acgtgtgagc tcggctagat attcgatttc
     6061 tttttgatga tcagaatcag cattacataa tatcgtgacg aaattctgct tatacaagat
     6121 atcctcaatt cctcgcatca gtgtactgaa gaatggattc gtgatatcag gaacgagtac
     6181 accaattgtt ttcgtttcct tggtaatcat ttgtctagca aaatagtcag gctgatagcc
     6241 taattcttct ttggcagcta aaactttctg aacagttttc ggactgaatt tctccgtatt
     6301 tccatttaat atcaaggaga cagttgtgat tgaaacgccg gaaagtttag cgacttcttt
     6361 gattgtgatc ttttttttca ccatgttcat actcctatct aaatcctgtg gttattttcc
     6421 catgaaatca aaaagaagta aatgaaaatc acgtgtgcca gtacttattt gctttgttgg
     6481 atagcttcga ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa tatctttttt
     6541 atgaacaatt gatgggattc tttgtcacga tcactgcaag catcttcagc aagcacgata
     6601 tggtacccgt gttgaaaggc atctaatgca gtagcatata caccgtttgt tgtcgctaca
     6661 ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt gaagatcaag agaagtacca
     6721 aaaaaagcac ttggattgta ttttgtgata gtcaacatat tatctgtatc tttcaaagaa
     6781 tccttcaaaa gtaaatcagt gaattctgga ggtacagaag cagtcattcc acccatatca
     6841 gtttgttgac tcaaataagg gaaagtggag atgtctacgt tcactaaaac tttcaaggca
     6901 gcggtacgtt tcaatgttct tactaacaaa tcattgtttg tcagaataga tgctgcagtg
     6961 tgtggagaga gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc gacaaaagca
     7021 gtttttgata aatcaaaaat catattttcc tttaaatcat tcatgatgtt ccttctttca
     7081 aataacaatg ttttttatgt aagtaaatca taaatcgttt atttgtcaac taaattaaag
     7141 gattcctttc tttagtctag agaaattcag tggtaaaata aataagtgtg agaaaacgca
     7201 ttcgatgcat agatatagcg agaaatatcg tgaaaatgga ggaatcagag tgaagtatgt
     7261 cgaagtaaaa aatgaataca aacgatacaa aatgggagaa acgaccatta cagcaaatga
     7321 cgatatttct ttttcaattg aaaaaggcga attagtgatt atacttggtc caagtggagc
     7381 aggaaagtca acgattctta atattttagg tgggatggat acacctgatg aaggtcagat
     7441 cattattgat gacacagata ttgcgcaatt ttcagataaa cagcttactg cttaccgacg
     7501 aacagatgta gggttcgttt tccaattcta caatctcgtt cctaatctaa cagcaaaaga
     7561 aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag aggtattgag
     7621 gcaagtgggc ctagctcatc gattgaataa ctttccgtcc caattatcag gaggcgaaca
     7681 gcagagggtt tcaattgctc gtgcactagc taaaaatccc aaacttcttt tatgtgatga
     7741 accgacaggc gcgctggatt ttgaaacggg caagcaagta ttgaaattat tgcaaaacgc
     7801 tagcagacaa cacggcaata ccgttttgat cattacacat aattctgctt tagcgcccat
     7861 cgctgataga gtcattcaca ttaatgatgc taaagtaagg tctgttgaat taaatgatca
     7921 tccttcatcc attgatgaga ttgtatggta gggggatttt atgaaaacaa gaacatatct
     7981 taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa tcatccttat
     8041 tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact gctggacctg tcatgcaaaa
     8101 aacgatggat cactatgttg gtacagcagg actttctgat ttgcaaatcg tttctactgg
     8161 tggcttgaca caaaaagata ttgctcaagc agaaaagatc ccagatgcaa aagtagaaac
     8221 aggaaaacag atctattatt ccaatactgg caaaaacgaa gtgattcaaa tattttctta
     8281 taacaaaaat aacaagcaga acaagctaca attaactgat ggaaaactcc cggaaaaacc
     8341 aaaccagctt gtattggatg aaaaagcgga agatgaaggg tataaaatcg gtgatattta
     8401 tcagattgat tcagatgaat tgaaagaaaa agagtatacc atcacgggct ttgtccgttc
     8461 tccattgttc atcaacaatt tggagagagg atacgcgaac gtcggaaatg gtagcgtcga
     8521 ttattttgtc tatttacctg aatccgtttt taaaacagat atttattctg tcttatatct
     8581 tgattttaca aacgtaaaga atttgaatac gtacagcaaa gcttacaaag ataaaatgga
     8641 gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga cccgaagaac gcttagaaga
     8701 gctgaaaaaa tcggccaacg aagaattaga accggcacag caaaaaatag cggatggcaa
     8761 agcacaaaca acgcaagcaa gaacacggtt agaagatgct aaaaaacagt tagcgcaaca
     8821 gtcagcaact atccagcagc ttcctgaagc gcaacgaaca atcgctgttc aaacattatc
     8881 tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta gttgagaaag aacgtgaact
     8941 ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa attgaaaatt taactaaacc
     9001 atcataccta tacaatgaac gttcagaaaa tgtcggtttt caagaatttg gtgatctgtc
     9061 tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc tttttcttta ttgccgcatt
     9121 gatcaccttt acgacaatga cacgtatggt agaagaaaac cgcagggaaa tcgggacatt
     9181 aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct atgcaagtct
     9241 agcctcaggg ataggtattg ttttaggaac gatattagga acgaatcttt tgccaaggat
     9301 catttttgag ctgtctaacg agcggtatga tattgggagt gcgcttattt tttatgactg
     9361 gccgccgatt ttacaagcag ctgccgcatt cttcattgcc gcatttggcg ctgcaatgat
     9421 cgttttattc aaagatttaa gagaacggcc tgctgcattg cttcaaccga aagcaccgaa
     9481 gccagggaaa cgcatttttc ttgaatacat cactccgctg tggtcacgcc ttagctttaa
     9541 tcaaaaaatc agttaccgaa atctcttccg atataagtca agaatgctta tggcgattat
     9601 cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag attctctaag
     9661 ttctgtttca gccaaacaat ttggaccgat cattgactat caagcaatcg ttacattgga
     9721 ttcatccacg gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc aagaaaaagt
     9781 aaatgagatc ctagctattc atactcaaac cgtagaattg agaaaaaaag gacatgcttc
     9841 gcaaagtctg acagtgatcg taccaagtaa aacagctgat ttggatcgtt ttatccattt
     9901 aactgatctt gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta ttacgcaaaa
     9961 agtagcagaa ctatttgact tatctacagg agatcatatt tcgctttatg atgaagatca
    10021 aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta gctttattta
    10081 tatgtcccct gaatattatg cttcagttac tgatcaatcg atgatggata atgcattttt
    10141 gctgcgtaca gataagatga caacaaaaga agaagaaatg ctttccgaga aactgcttgc
    10201 cacagacgct gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc aagaagaatc
    10261 aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac tgctggcttt
    10321 tatcgtcttg tataatctaa ctaatatcaa tatttccgag cgtgtacgcg aattatctac
    10381 aataaaagtc cttggatttt ttgataaaga agtcacgatg tacattgttc gagaaaatat
    10441 catctttact cttttaggaa tcatcggagg atttggtgta ggatatatat tgactgactt
    10501 catcttgcaa caagcgtcta tggagaatgt catctttcca ttagtgatca catggtgggc
    10561 ttatgtctta tcagcaggat taactatcgt atttacggtt attgtgatga ttgttaccca
    10621 tttcaaatta aagcatatcg atatgattga tgcgttgaaa tcgaatgaat aaaaacagag
    10681 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggcaaaa tagtttttca
    10741 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacca tgaatcagag
    10801 gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat tagctcctca
    10861 tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac catttataga
    10921 agaaacaaga atattttgct attttaaaca agaggataga caaatacatg tctgatctct
    10981 tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta caaacgcttt
    11041 cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag gtaataaatc
    11101 agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc aaatctatcc
    11161 caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa
    11221 actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta tttatccaag
    11281 ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc ctatctttgg
    11341 tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga tagaaatcat
    11401 gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa aagcactagc
    11461 aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg attgggtatc
    11521 aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt atttgcactt
    11581 atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag aggaactatt
    11641 caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa
    11701 tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca aacctgaata
    11761 tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa cgtttgggac
    11821 ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa attgtatctt
    11881 gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc atttgaaagt
    11941 cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag aattaaagcg
    12001 actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg ctctattttt
    12061 taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc agtatcgaaa
    12121 acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc cctatattta
    12181 tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg actatattga
    12241 cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag aaaaagaagc
    12301 gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc aatggtcaga
    12361 cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag gcaagctaga
    12421 agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct attacaagaa
    12481 attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc atgcttatga
    12541 agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc agctgcttgt
    12601 attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat tcacagaagc
    12661 caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg agctaaaacc
    12721 ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga
    12781 gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt cagaaaatga
    12841 agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt tgaatttacg
    12901 gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa aaataacgcc
    12961 cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa tcggatggat
    13021 ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa gttgtacggc
    13081 aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg caatgatcaa
    13141 acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca acccagcaga
    13201 aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc agtagactgc
    13261 tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt gttgattagc
    13321 aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg ctaaaactga
    13381 ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac cagtaaataa
    13441 agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg cgccactttc
    13501 ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag aagcgaaaaa
    13561 ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa
    13621 atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt caagaaaagg
    13681 aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa cgataatgac
    13741 tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca aaagataata
    13801 caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc cgacaggctt
    13861 gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga taactattcc
    13921 taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt
    13981 ttcttttttc actctttttc ctcctttatc tataaattca ttatagacaa aagcgggtat
    14041 aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata taaactttct
    14101 catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa attaaatcct
    14161 gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc aaatgaaaga
    14221 agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc atgctggttt
    14281 tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat
    14341 tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc gtcggggatg
    14401 aaggagaagt agagttattg attccagcag ggactgattc ccacgattat gaaccttctg
    14461 ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat gaaaacatgg
    14521 aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat acgataaaag
    14581 cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat gaccatgaac
    14641 acggggaaga aggccataca catgagctag acccacatgt atggctagct ccaagtttag
    14701 caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca gaaaaacagg
    14761 aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc catcagcttt
    14821 atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat gctgcattca
    14881 actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt tcttctagtg
    14941 aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa catggaatca
    15001 actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta gcaaacgaag
    15061 caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa
    15121 atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa aaaacgacag
    15181 agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc gtctacaacg
    15241 gttattttga agacgaggat gtacaagatc gctctctttc agattgggca ggaacttggc
    15301 aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa tataaagaaa
    15361 aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag ggctatcaaa
    15421 cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt aagaatggaa
    15481 caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac gaggcaggaa
    15541 accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct tttagatata
    15601 ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat ttatatgcag
    15661 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
    15721 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
    15781 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
    15841 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
    15901 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
    15961 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
    16021 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
    16081 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
    16141 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
    16201 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
    16261 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
    16321 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
    16381 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
    16441 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
    16501 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
    16561 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
    16621 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
    16681 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
    16741 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
    16801 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
    16861 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
    16921 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
    16981 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
    17041 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
    17101 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
    17161 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
    17221 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
    17281 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
    17341 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
    17401 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
    17461 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
    17521 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
    17581 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
    17641 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
    17701 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
    17761 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
    17821 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
    17881 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
    17941 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
    18001 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
    18061 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
    18121 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
    18181 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
    18241 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
    18301 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
    18361 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
    18421 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
    18481 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
    18541 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
    18601 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
    18661 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
    18721 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
    18781 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
    18841 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
    18901 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
    18961 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
    19021 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
    19081 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
    19141 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
    19201 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
    19261 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
    19321 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
    19381 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
    19441 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
    19501 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
    19561 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
    19621 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
    19681 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
    19741 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
    19801 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
    19861 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
    19921 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
    19981 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
    20041 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
    20101 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
    20161 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
    20221 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
    20281 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
    20341 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
    20401 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
    20461 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
    20521 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
    20581 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
    20641 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
    20701 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
    20761 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
    20821 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
    20881 ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact aaaatcatat
    20941 cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa
    21001 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
    21061 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
    21121 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
    21181 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
    21241 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
    21301 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
    21361 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
    21421 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
    21481 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
    21541 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
    21601 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
    21661 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
    21721 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
    21781 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
    21841 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
    21901 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
    21961 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
    22021 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
    22081 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
    22141 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
    22201 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
    22261 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
    22321 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
    22381 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
    22441 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
    22501 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
    22561 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
    22621 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
    22681 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
    22741 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
    22801 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
    22861 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
    22921 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
    22981 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
    23041 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg
    23101 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
    23161 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
    23221 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
    23281 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc
    23341 ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag gtgtgacaag
    23401 aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc aaaatagaga
    23461 atatatagaa aaattctcat tattcaaacg taaataatca gttattttga ttttttttat
    23521 attttattag ctaataattg gtattttaaa agaaattact gtagaattgg attataatta
    23581 aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc ctgtagaaaa
    23641 gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat ttttgccgaa
    23701 ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg agttcaagat
    23761 attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa cgaattaact
    23821 ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg acctattttg
    23881 attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc atttgtccct
    23941 cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa catcttaaat
    24001 tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact gtgtaacaag
    24061 tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg ttgacttaga
    24121 agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag aagtgcaagc
    24181 agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg acctttcctg
    24241 tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc tcagacgtcc
    24301 tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga tggaacagct
    24361 tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt catacagttg
    24421 taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact ttatttttct
    24481 actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg gatatagagt
    24541 taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg ttttaagtag
    24601 caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat gttagaaatg
    24661 acatgataag ataactgagt tgttaaaaaa gaaccacttg tgaagcttga ttctatcgaa
    24721 aaaaacttca aaaaagttct tgacaaataa caaatgataa gttatgataa atgagttgct
    24781 gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca attacttgtc
    24841 tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga acactcctga
    24901 tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag ggcgtcttga
    24961 ttcaattcaa gacaacaaac a
//
LOCUS       DAZKMZ010000035        24453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000035 DAZKMZ010000000
VERSION     DAZKMZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.035"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG8M5_001521"
     CDS             complement(<1..329)
                     /locus_tag="ACG8M5_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358777.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="ACG8M5_001522"
     CDS             complement(343..600)
                     /locus_tag="ACG8M5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358778.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="ACG8M5_001523"
     CDS             complement(795..1337)
                     /locus_tag="ACG8M5_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0358779.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="ACG8M5_001524"
     CDS             complement(1358..2449)
                     /locus_tag="ACG8M5_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0358780.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="ACG8M5_001525"
     CDS             complement(2457..4667)
                     /locus_tag="ACG8M5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0358781.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="ACG8M5_001526"
     CDS             complement(4683..7211)
                     /locus_tag="ACG8M5_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0358782.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="ACG8M5_001527"
     CDS             complement(7263..7673)
                     /locus_tag="ACG8M5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0358783.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="ACG8M5_001528"
     CDS             complement(7686..7904)
                     /locus_tag="ACG8M5_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0358784.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="ACG8M5_001529"
     CDS             complement(7901..8893)
                     /locus_tag="ACG8M5_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0358785.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="ACG8M5_001530"
     CDS             complement(8972..9883)
                     /locus_tag="ACG8M5_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0358786.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="ACG8M5_001531"
     CDS             complement(9902..10246)
                     /locus_tag="ACG8M5_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358787.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10544)
                     /locus_tag="ACG8M5_001532"
     CDS             complement(10266..10544)
                     /locus_tag="ACG8M5_001532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358788.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10903..11403)
                     /locus_tag="ACG8M5_001533"
     CDS             complement(10903..11403)
                     /locus_tag="ACG8M5_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358789.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="ACG8M5_001534"
     CDS             complement(11404..11949)
                     /locus_tag="ACG8M5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358790.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="ACG8M5_001535"
     CDS             complement(11946..13202)
                     /locus_tag="ACG8M5_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0358791.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="ACG8M5_001536"
     CDS             complement(13563..13874)
                     /locus_tag="ACG8M5_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358792.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15372)
                     /locus_tag="ACG8M5_001537"
     CDS             complement(13897..15372)
                     /locus_tag="ACG8M5_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0358793.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="ACG8M5_001538"
     CDS             complement(15821..16273)
                     /locus_tag="ACG8M5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358794.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="ACG8M5_001539"
     CDS             complement(16275..16640)
                     /locus_tag="ACG8M5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358795.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="ACG8M5_001540"
     CDS             complement(16739..21022)
                     /locus_tag="ACG8M5_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0358796.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="ACG8M5_001541"
     CDS             complement(21169..21408)
                     /locus_tag="ACG8M5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358797.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21740)
                     /locus_tag="ACG8M5_001542"
                     /pseudo
     CDS             complement(21498..21740)
                     /locus_tag="ACG8M5_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21853..22104)
                     /locus_tag="ACG8M5_001543"
     CDS             complement(21853..22104)
                     /locus_tag="ACG8M5_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358798.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..23226
                     /locus_tag="ACG8M5_001544"
     CDS             22267..23226
                     /locus_tag="ACG8M5_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0358799.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23478..>24453
                     /locus_tag="ACG8M5_001545"
     CDS             23478..>24453
                     /locus_tag="ACG8M5_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0358800.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         7351 a         5234 c         3727 g         8141 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt gtctaactta
    23221 atttgacaaa cgaaattttt aggtcgaaag cgaaaaagaa tcgttgttat cttaagaaat
    23281 gctagactta acgcggcttt ttcatgatca ttttctgaaa cgaacagttt tgtcattttt
    23341 aaaagttgtt ttgaaacatt tttgtaacat aactattata tcgttgacac actgctatgg
    23401 tacaattttt atatcgttaa gaaagactta aatatattta gatatttatt aaattaagcc
    23461 agtcggagga atgaagagtg aaaaagagtt taatatcagc agtaatggta tgttcaatga
    23521 ccttgactgc cgtagcatca ccgatcgctg ccgcggcgga cgattttgat tctcagatac
    23581 aacagcaaga tcaaaagatt gcagacttga aaaatcaaca agcagatgca caatcacaaa
    23641 tcgatgcctt agaaagccaa gtttctgaaa tcaacacaca agcacaagac ctattagcaa
    23701 aacaagatac attgcgtcaa gaatctgcac aattagtaaa agacattgca gatttacaag
    23761 aacgtattga aaaacgtgaa gatacaatcc aaaaacaagc acgcgaagca caagtaagca
    23821 acacaagctc aaactacatc gatgctgtat tgaatgctga ttcattagca gatgctattg
    23881 gacgcgttca agcaatgaca acgatggtaa aagcaaacaa cgacttgatg gaacaacaaa
    23941 aacaagataa aaaagctgta gaagacaaaa aagctgaaaa cgatgctaaa ctaaaagaat
    24001 tagctgaaaa ccaagctgct ttggaatctc aaaaaggcga tcttttatct aaacaagctg
    24061 atttgaacgt actgaaaact tcattagctg ctgaacaagc aactgctgaa gacaagaaag
    24121 ctgacttgaa ccgtcaaaaa gcagaagctg aagcagagca agctcgtatt cgtgaacaac
    24181 aacgtctagc agaacaagct cgtcaacaag ctgcacaaga aaaagctgaa aaagaagcac
    24241 gcgaacaagc agaggctgaa gcacaagcta ctcaagcttc atctactgca caaagctcag
    24301 caacagaaga atcatcagct acacaatctt caatgactga agaatcatca tcagctacac
    24361 aatcttcagc aacagaagaa tcaacaacac ctgaatcttc aacagaagaa tcaacagcgc
    24421 ctgaatcttc agcaacagaa gaatcaacaa cag
//
LOCUS       DAZKMZ010000036        24274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000036 DAZKMZ010000000
VERSION     DAZKMZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.036"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG8M5_001546"
     CDS             complement(55..789)
                     /locus_tag="ACG8M5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0358801.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG8M5_001547"
     CDS             1116..1526
                     /locus_tag="ACG8M5_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0358802.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG8M5_001548"
     CDS             1519..2220
                     /locus_tag="ACG8M5_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0358803.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8M5_001549"
     CDS             2220..3806
                     /locus_tag="ACG8M5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358804.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8M5_001550"
     CDS             3825..4046
                     /locus_tag="ACG8M5_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0358805.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8M5_001551"
     CDS             4043..4804
                     /locus_tag="ACG8M5_001551"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0358806.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8M5_001552"
     CDS             5184..5693
                     /locus_tag="ACG8M5_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0358807.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8M5_001553"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8M5_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0358808.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8M5_001554"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8M5_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0358809.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8M5_001555"
     CDS             7320..8171
                     /locus_tag="ACG8M5_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0358810.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8M5_001556"
     CDS             8199..8834
                     /locus_tag="ACG8M5_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0358811.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8M5_001557"
     CDS             8854..9627
                     /locus_tag="ACG8M5_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0358812.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8M5_001558"
     CDS             10035..10832
                     /locus_tag="ACG8M5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0358813.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8M5_001559"
     CDS             10810..11223
                     /locus_tag="ACG8M5_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0358814.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8M5_001560"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8M5_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0358815.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8M5_001561"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8M5_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0358816.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8M5_001562"
     CDS             16000..16560
                     /locus_tag="ACG8M5_001562"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0358817.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8M5_001563"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8M5_001563"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0358818.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8M5_001564"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8M5_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0358819.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8M5_001565"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8M5_001565"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0358820.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8M5_001566"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8M5_001566"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0358821.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8M5_001567"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8M5_001567"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0358822.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8M5_001568"
     CDS             21907..22485
                     /locus_tag="ACG8M5_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0358823.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8M5_001569"
     CDS             22537..23019
                     /locus_tag="ACG8M5_001569"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0358824.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8M5_001570"
     CDS             23136..23510
                     /locus_tag="ACG8M5_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358825.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24274)
                     /locus_tag="ACG8M5_001571"
     CDS             complement(23885..>24274)
                     /locus_tag="ACG8M5_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0358826.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7906 a         4353 c         5198 g         6817 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
    24061 actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa tcataaaaag
    24121 ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt ttcttccatt
    24181 tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct tgataaacag
    24241 ccttaaagtc atcacagatt tcttttcggt cttt
//
LOCUS       DAZKMZ010000037        23633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000037 DAZKMZ010000000
VERSION     DAZKMZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.037"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8M5_001572"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8M5_001572"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0358827.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8M5_001573"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8M5_001573"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0358828.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8M5_001574"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8M5_001574"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0358829.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8M5_001575"
     CDS             2925..3533
                     /locus_tag="ACG8M5_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0358830.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8M5_001576"
     CDS             3858..4181
                     /locus_tag="ACG8M5_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0358831.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8M5_001577"
     CDS             4363..5733
                     /locus_tag="ACG8M5_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0358832.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8M5_001578"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8M5_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0358833.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8M5_001579"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8M5_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0358834.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8M5_001580"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8M5_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0358835.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8M5_001581"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8M5_001581"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0358836.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8M5_001582"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8M5_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0358837.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8M5_001583"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8M5_001583"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0358838.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8M5_001584"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8M5_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0358839.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8M5_001585"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8M5_001585"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0358840.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8M5_001586"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8M5_001586"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0358841.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8M5_001587"
     CDS             16339..16635
                     /locus_tag="ACG8M5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358842.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8M5_001588"
     CDS             17203..18795
                     /locus_tag="ACG8M5_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0358843.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8M5_001589"
     CDS             18808..19548
                     /locus_tag="ACG8M5_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358844.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8M5_001590"
     CDS             19716..21065
                     /locus_tag="ACG8M5_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0358845.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8M5_001591"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8M5_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0358846.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8M5_001592"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8M5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0358847.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6763 a         4760 c         4501 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttc
//
LOCUS       DAZKMZ010000038        23010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000038 DAZKMZ010000000
VERSION     DAZKMZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.038"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..512
                     /locus_tag="ACG8M5_001593"
     CDS             144..512
                     /locus_tag="ACG8M5_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0358848.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            692..1633
                     /locus_tag="ACG8M5_001594"
     CDS             692..1633
                     /locus_tag="ACG8M5_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0358849.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1702..2433
                     /locus_tag="ACG8M5_001595"
     CDS             1702..2433
                     /locus_tag="ACG8M5_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0358850.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2577..4757
                     /locus_tag="ACG8M5_001596"
     CDS             2577..4757
                     /locus_tag="ACG8M5_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0358851.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4777..5259
                     /locus_tag="ACG8M5_001597"
     CDS             4777..5259
                     /locus_tag="ACG8M5_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0358852.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5275..6036
                     /locus_tag="ACG8M5_001598"
     CDS             5275..6036
                     /locus_tag="ACG8M5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0358853.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6167..7501
                     /gene="murC"
                     /locus_tag="ACG8M5_001599"
     CDS             6167..7501
                     /gene="murC"
                     /locus_tag="ACG8M5_001599"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0358854.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7808..8293
                     /locus_tag="ACG8M5_001600"
     CDS             7808..8293
                     /locus_tag="ACG8M5_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0358855.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8286..8969
                     /locus_tag="ACG8M5_001601"
     CDS             8286..8969
                     /locus_tag="ACG8M5_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0358856.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9095..11740
                     /gene="polA"
                     /locus_tag="ACG8M5_001602"
     CDS             9095..11740
                     /gene="polA"
                     /locus_tag="ACG8M5_001602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0358857.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11785..12621
                     /gene="mutM"
                     /locus_tag="ACG8M5_001603"
     CDS             11785..12621
                     /gene="mutM"
                     /locus_tag="ACG8M5_001603"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0358858.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12585..13214
                     /gene="coaE"
                     /locus_tag="ACG8M5_001604"
     CDS             12585..13214
                     /gene="coaE"
                     /locus_tag="ACG8M5_001604"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0358859.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13266..13598
                     /locus_tag="ACG8M5_001605"
     CDS             13266..13598
                     /locus_tag="ACG8M5_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0358860.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13910..14401
                     /gene="nrdR"
                     /locus_tag="ACG8M5_001606"
     CDS             13910..14401
                     /gene="nrdR"
                     /locus_tag="ACG8M5_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0358861.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14424..15797
                     /locus_tag="ACG8M5_001607"
     CDS             14424..15797
                     /locus_tag="ACG8M5_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0358862.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15797..16723
                     /gene="dnaI"
                     /locus_tag="ACG8M5_001608"
     CDS             15797..16723
                     /gene="dnaI"
                     /locus_tag="ACG8M5_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0358863.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16852..17292
                     /locus_tag="ACG8M5_001609"
     CDS             16852..17292
                     /locus_tag="ACG8M5_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0358864.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17436..18137
                     /locus_tag="ACG8M5_001610"
     CDS             17436..18137
                     /locus_tag="ACG8M5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0358865.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18134..19255
                     /locus_tag="ACG8M5_001611"
     CDS             18134..19255
                     /locus_tag="ACG8M5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0358866.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19270..20502
                     /gene="pepT"
                     /locus_tag="ACG8M5_001612"
     CDS             19270..20502
                     /gene="pepT"
                     /locus_tag="ACG8M5_001612"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0358867.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20634..21542
                     /locus_tag="ACG8M5_001613"
     CDS             20634..21542
                     /locus_tag="ACG8M5_001613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0358868.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21577..21849
                     /locus_tag="ACG8M5_001614"
     CDS             21577..21849
                     /locus_tag="ACG8M5_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0358869.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21860..22906
                     /locus_tag="ACG8M5_001615"
     CDS             21860..22906
                     /locus_tag="ACG8M5_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0358870.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7759 a         4006 c         5042 g         6203 t
ORIGIN      
        1 aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac gattctgttg
       61 agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga gaatgttaga
      121 acagatcgtt taggaggaaa cagatgttta cgaatgaaat aaaaatcatg ctttatgtgg
      181 acgatgtaga aaaaaatgca acgttttgga gagcagttgg ctttgcggaa aaagatcgtc
      241 aagaaatgga tgggacgctt ataattgaag taggagttga ggacagtcaa acctcatttc
      301 tgttatatga tcgagcattt gttgagtccc attcacctgg cacagctact agtgccccat
      361 ctttgatgtt ttacagtaaa gatatctttg gactatataa aaaaatgcag acgcaatcag
      421 tcacattagg tgatatggta cagcttggag aagaatatgt ctttaacttt gctgatcccg
      481 atggcaacta ctttgcggtt acaggtaaat aatagaaata aagactttgt ttttctgtgc
      541 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
      601 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
      661 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
      721 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
      781 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
      841 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
      901 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
      961 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
     1021 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
     1081 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
     1141 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
     1201 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
     1261 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
     1321 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
     1381 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
     1441 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
     1501 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     1561 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc
     1621 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     1681 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     1741 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     1801 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     1861 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     1921 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     1981 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     2041 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     2101 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     2161 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     2221 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     2281 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     2341 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     2401 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     2461 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     2521 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     2581 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     2641 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     2701 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     2761 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     2821 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     2881 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     2941 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     3001 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     3061 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     3121 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     3181 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     3241 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     3301 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     3361 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     3421 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     3481 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     3541 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     3601 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     3661 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     3721 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     3781 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     3841 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     3901 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     3961 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     4021 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     4081 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     4141 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     4201 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     4261 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     4321 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     4381 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     4441 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     4501 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     4561 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     4621 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     4681 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     4741 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     4801 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     4861 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     4921 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     4981 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
     5041 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
     5101 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
     5161 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
     5221 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
     5281 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
     5341 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
     5401 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
     5461 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
     5521 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
     5581 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
     5641 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
     5701 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
     5761 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
     5821 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
     5881 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
     5941 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
     6001 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
     6061 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
     6121 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
     6181 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
     6241 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
     6301 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
     6361 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
     6421 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
     6481 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
     6541 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
     6601 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
     6661 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
     6721 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
     6781 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
     6841 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
     6901 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
     6961 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
     7021 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
     7081 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
     7141 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
     7201 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
     7261 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
     7321 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
     7381 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
     7441 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
     7501 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
     7561 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
     7621 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
     7681 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
     7741 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
     7801 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
     7861 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
     7921 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
     7981 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
     8041 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
     8101 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
     8161 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
     8221 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
     8281 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
     8341 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
     8401 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
     8461 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
     8521 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
     8581 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
     8641 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
     8701 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
     8761 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
     8821 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
     8881 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
     8941 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
     9001 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
     9061 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
     9121 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
     9181 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
     9241 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
     9301 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
     9361 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
     9421 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
     9481 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
     9541 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
     9601 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
     9661 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
     9721 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
     9781 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
     9841 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
     9901 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
     9961 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
    10021 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
    10081 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
    10141 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
    10201 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
    10261 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
    10321 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
    10381 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
    10441 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
    10501 actggcagaa aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    10561 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    10621 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    10681 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    10741 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    10801 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    10861 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    10921 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    10981 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    11041 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    11101 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    11161 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    11221 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    11281 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    11341 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    11401 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    11461 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    11521 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    11581 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    11641 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    11701 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    11761 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    11821 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    11881 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    11941 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    12001 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    12061 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    12121 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    12181 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    12241 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    12301 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    12361 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    12421 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    12481 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    12541 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    12601 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    12661 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    12721 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    12781 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    12841 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    12901 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    12961 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    13021 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc
    13081 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    13141 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    13201 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    13261 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    13321 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    13381 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    13441 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    13501 gtaaaacacc gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc
    13561 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    13621 acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag atgactaaat
    13681 cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa aattgaaatt
    13741 tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct tatatccgtg
    13801 ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca actagttgta
    13861 aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc
    13921 cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg atggacgtgc
    13981 gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat ttgaacgaat
    14041 tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga
    14101 taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta tggaacaaat
    14161 ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc
    14221 gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg aaatcgctta
    14281 tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct taaaagagtt
    14341 gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag aaaacaatta
    14401 agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata
    14461 tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc
    14521 tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa
    14581 tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag
    14641 gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg
    14701 atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac
    14761 cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag
    14821 ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg catacagcag
    14881 agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt
    14941 acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag
    15001 tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa
    15061 aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc
    15121 tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg
    15181 aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg
    15241 tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg
    15301 aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga
    15361 aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt
    15421 tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga
    15481 attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg
    15541 caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc
    15601 aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca
    15661 agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac
    15721 agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa
    15781 aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg
    15841 caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca
    15901 agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc
    15961 taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat
    16021 cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt
    16081 accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc
    16141 aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg
    16201 acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa
    16261 agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt
    16321 aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc
    16381 gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga
    16441 tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag
    16501 ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc
    16561 aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca
    16621 acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac
    16681 aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa
    16741 ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc
    16801 ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct
    16861 attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca
    16921 gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt
    16981 acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa
    17041 gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg
    17101 aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata
    17161 agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg
    17221 ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca
    17281 ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa
    17341 aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt
    17401 ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc
    17461 gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag
    17521 atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg
    17581 gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag
    17641 aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta
    17701 ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg
    17761 gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag
    17821 agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac
    17881 ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta
    17941 cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct
    18001 tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac
    18061 aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg
    18121 aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg
    18181 tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa
    18241 gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat
    18301 caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg
    18361 gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc
    18421 cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg actggttttg
    18481 tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa
    18541 aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc
    18601 agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg
    18661 tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca
    18721 agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca
    18781 agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga
    18841 accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga
    18901 ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc
    18961 aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt
    19021 ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca
    19081 tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga
    19141 atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg
    19201 ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga
    19261 gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca
    19321 cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa
    19381 gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac
    19441 ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt
    19501 atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa
    19561 aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca
    19621 aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt
    19681 acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc
    19741 ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa
    19801 gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat
    19861 acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca
    19921 aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat
    19981 gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa
    20041 acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct
    20101 tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa
    20161 atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat
    20221 atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa
    20281 ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga
    20341 ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga
    20401 ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca
    20461 gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct
    20521 tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac
    20581 aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa
    20641 agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct
    20701 ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg
    20761 gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt
    20821 tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat
    20881 tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc
    20941 ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga
    21001 aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct
    21061 tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct
    21121 ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa
    21181 cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg
    21241 tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa
    21301 cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag
    21361 gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag
    21421 cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg
    21481 aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt
    21541 agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct
    21601 ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa
    21661 catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg
    21721 ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa
    21781 aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt
    21841 ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct
    21901 gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga
    21961 tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg
    22021 gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc
    22081 gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt
    22141 ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa
    22201 gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat
    22261 cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc
    22321 aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt
    22381 ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt
    22441 taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag
    22501 ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa
    22561 tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc
    22621 taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt
    22681 tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc
    22741 agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca gttcctttat
    22801 cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag tgatcaaaga
    22861 agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct tttataagat
    22921 ctaccctttt tatcaaatca aagaaaaacg ttatggataa gataatttta cgtttgtttg
    22981 gctctttttt tatatacata caatcaattt
//
LOCUS       DAZKMZ010000039        22666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000039 DAZKMZ010000000
VERSION     DAZKMZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.039"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8M5_001616"
     CDS             133..3660
                     /locus_tag="ACG8M5_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0358871.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8M5_001617"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8M5_001617"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0358872.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8M5_001618"
     CDS             7448..7789
                     /locus_tag="ACG8M5_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0358873.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8M5_001619"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8M5_001619"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0358874.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8M5_001620"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8M5_001620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0358875.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8M5_001621"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8M5_001621"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0358876.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8M5_001622"
     CDS             13000..13737
                     /locus_tag="ACG8M5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0358877.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8M5_001623"
     CDS             13751..14566
                     /locus_tag="ACG8M5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0358878.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8M5_001624"
     CDS             14579..15226
                     /locus_tag="ACG8M5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0358879.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8M5_001625"
     CDS             15242..15898
                     /locus_tag="ACG8M5_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0358880.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8M5_001626"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8M5_001626"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0358881.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8M5_001627"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8M5_001627"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0358882.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8M5_001628"
     CDS             18240..18758
                     /locus_tag="ACG8M5_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0358883.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8M5_001629"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8M5_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0358884.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8M5_001630"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8M5_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0358885.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8M5_001631"
     CDS             20691..21743
                     /locus_tag="ACG8M5_001631"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0358886.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8M5_001632"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8M5_001632"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0358887.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7552 a         3950 c         4826 g         6338 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataat
//
LOCUS       DAZKMZ010000040        22422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000040 DAZKMZ010000000
VERSION     DAZKMZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.040"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8M5_001633"
     CDS             6..305
                     /locus_tag="ACG8M5_001633"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0358888.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8M5_001634"
     CDS             378..1739
                     /locus_tag="ACG8M5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0358889.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8M5_001635"
     CDS             1761..2021
                     /locus_tag="ACG8M5_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358890.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8M5_001636"
     CDS             2035..2787
                     /locus_tag="ACG8M5_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0358891.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8M5_001637"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8M5_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0358892.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8M5_001638"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8M5_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0358893.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8M5_001639"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8M5_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0358894.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M5_001640"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M5_001640"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0358895.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8M5_001641"
     CDS             7161..8558
                     /locus_tag="ACG8M5_001641"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0358896.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8M5_001642"
     CDS             8639..9079
                     /locus_tag="ACG8M5_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0358897.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8M5_001643"
     CDS             9227..9553
                     /locus_tag="ACG8M5_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0358898.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8M5_001644"
     CDS             9554..10303
                     /locus_tag="ACG8M5_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0358899.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8M5_001645"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8M5_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0358900.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8M5_001646"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8M5_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358901.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8M5_001647"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8M5_001647"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0358902.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8M5_001648"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8M5_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0358903.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8M5_001649"
     CDS             14770..17436
                     /locus_tag="ACG8M5_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0358904.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8M5_001650"
     CDS             17800..18552
                     /locus_tag="ACG8M5_001650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0358905.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8M5_001651"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8M5_001651"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0358906.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8M5_001652"
     CDS             19913..22354
                     /locus_tag="ACG8M5_001652"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0358907.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3933 c         4673 g         6618 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt tt
//
LOCUS       DAZKMZ010000041        22112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000041 DAZKMZ010000000
VERSION     DAZKMZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.041"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..2017
                     /gene="tdc"
                     /locus_tag="ACG8M5_001653"
     CDS             182..2017
                     /gene="tdc"
                     /locus_tag="ACG8M5_001653"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0358908.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2322..3800
                     /locus_tag="ACG8M5_001654"
     CDS             2322..3800
                     /locus_tag="ACG8M5_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0358909.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3766..5097
                     /locus_tag="ACG8M5_001655"
     CDS             3766..5097
                     /locus_tag="ACG8M5_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0358910.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5211..7763
                     /locus_tag="ACG8M5_001656"
     CDS             5211..7763
                     /locus_tag="ACG8M5_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0358911.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7770..9209)
                     /locus_tag="ACG8M5_001657"
     CDS             complement(7770..9209)
                     /locus_tag="ACG8M5_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358912.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9184..9366
                     /locus_tag="ACG8M5_001658"
     CDS             9184..9366
                     /locus_tag="ACG8M5_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358913.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9555..9917
                     /locus_tag="ACG8M5_001659"
     CDS             9555..9917
                     /locus_tag="ACG8M5_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0358914.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10104..10961)
                     /locus_tag="ACG8M5_001660"
     CDS             complement(10104..10961)
                     /locus_tag="ACG8M5_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0358915.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11081..11992
                     /locus_tag="ACG8M5_001661"
     CDS             11081..11992
                     /locus_tag="ACG8M5_001661"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0358916.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11999..13645
                     /gene="mdcA"
                     /locus_tag="ACG8M5_001662"
     CDS             11999..13645
                     /gene="mdcA"
                     /locus_tag="ACG8M5_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0358917.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13747..14046
                     /locus_tag="ACG8M5_001663"
     CDS             13747..14046
                     /locus_tag="ACG8M5_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0358918.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14036..15712
                     /gene="mdcD"
                     /locus_tag="ACG8M5_001664"
     CDS             14036..15712
                     /gene="mdcD"
                     /locus_tag="ACG8M5_001664"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0358919.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15716..16309
                     /locus_tag="ACG8M5_001665"
     CDS             15716..16309
                     /locus_tag="ACG8M5_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0358920.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16333..17409
                     /locus_tag="ACG8M5_001666"
     CDS             16333..17409
                     /locus_tag="ACG8M5_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0358921.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17434..18303
                     /gene="mdcB"
                     /locus_tag="ACG8M5_001667"
     CDS             17434..18303
                     /gene="mdcB"
                     /locus_tag="ACG8M5_001667"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0358922.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18414..19178
                     /locus_tag="ACG8M5_001668"
     CDS             18414..19178
                     /locus_tag="ACG8M5_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0358923.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19667..21760
                     /locus_tag="ACG8M5_001669"
     CDS             19667..21760
                     /locus_tag="ACG8M5_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0358924.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21785..22087
                     /locus_tag="ACG8M5_001670"
     CDS             21785..22087
                     /locus_tag="ACG8M5_001670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0358925.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7163 a         3783 c         4714 g         6452 t
ORIGIN      
        1 tcgctttttt gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa
       61 tctacgtgga tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg
      121 tgttacagat aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa
      181 aatgaaagat atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt
      241 ttataaaatg ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat
      301 tccttcagat atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac
      361 ccgcgaccat atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat
      421 cccatggcat tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc
      481 tttattagct tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc
      541 tatggcaact tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat
      601 gacagacgga tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg
      661 gtacgcccgt tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt
      721 gaaaaaaatg tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac
      781 cgaaagcttt acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa
      841 aaatatccaa agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa
      901 ggcgttagat atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga
      961 ttatcgaatg gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat
     1021 cccaattcta ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt
     1081 agataaaatc gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca
     1141 tgttgatgcc gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt
     1201 tgtgccgtat gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt
     1261 aacgattgac aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac
     1321 gatagatcct cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca
     1381 taaaaatatg agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa
     1441 ggctccagat atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc
     1501 cgcagtctgg acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat
     1561 tggggcttca atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac
     1621 tgttaaagga aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa
     1681 ttgggtattc aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa
     1741 aatgttcgat cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc
     1801 tcatactaca tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat
     1861 gggtctaacg aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat
     1921 catgactcct tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa
     1981 agcaatggaa aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat
     2041 aacaagagac atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc
     2101 ctgtttttca tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga
     2161 tcgttcgttt catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat
     2221 actgggtttt gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa
     2281 gaaaacaaaa acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat
     2341 caaagcaaat cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac
     2401 gaaatattcc agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag
     2461 tattattgta cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc
     2521 aaaaaggtgg tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg
     2581 tctcatggct gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg
     2641 ctccgttgtt tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat
     2701 tagttgtcat tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg
     2761 caaaaatcgg cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc
     2821 tcttattagg gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca
     2881 gtttttcatg ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg
     2941 cgccgatcat gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg
     3001 aaaatccagt taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg
     3061 tcaatatttt gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga
     3121 ataatatcgc gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg
     3181 tgaatctgtt tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct
     3241 caggaccagc aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca
     3301 atttttggaa gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca
     3361 ttatctcaat ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga
     3421 tgctcgtaaa ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag
     3481 ttttgagatt gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag
     3541 agaaaaagag caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat
     3601 tttctgtcgg actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca
     3661 tcagtgtcat tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg
     3721 aacaggaagt cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca
     3781 atgaaacgga ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga
     3841 acaggattat ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc
     3901 gtttatttga tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt
     3961 ttagcaaata gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag
     4021 tgggcatttt ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa
     4081 ttaacagcaa taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc
     4141 ggaggtagcg cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt
     4201 gaattggaat cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg
     4261 ggtataggat tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt
     4321 ggacagatcg atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg
     4381 gttttattca gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat
     4441 ccgcaaaaga atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat
     4501 ataggaacga ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa
     4561 agtcctttag tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat
     4621 ctaatgattt tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg
     4681 attttatatc aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag
     4741 aaaggcgtac ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt
     4801 ctacattttt ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg
     4861 agttgttttc tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa
     4921 aagaaacgga acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca
     4981 attgctttca gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc
     5041 tggtttgtat tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc
     5101 cagatacatt ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa
     5161 aaaacttccc tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt
     5221 acagattgac ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc
     5281 aagaagaacg acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc
     5341 agacaaaacg atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga
     5401 tcattgcctc ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat
     5461 tcgtagtgat cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat
     5521 tgaatggttt gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag
     5581 aaaaaatccc tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg
     5641 tcgttccagc agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt
     5701 tgacgggaga atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga
     5761 tcggcgacca gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg
     5821 gtgtcgttgt agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat
     5881 cggtcgaaca acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt
     5941 ttcaaggcat tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta
     6001 tggattggca tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag
     6061 gattgccagc tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa
     6121 aggcgatcat caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt
     6181 cagataagac aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag
     6241 aagaagcctg cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac
     6301 agaaaacaga aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa
     6361 cagccaatct acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat
     6421 atatggcaac gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag
     6481 aagtattatt tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag
     6541 ctgcagaatt tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt
     6601 cgaaacagga actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat
     6661 tgatcgatcc gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga
     6721 tctcagtgaa aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa
     6781 ttggcttaaa acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg
     6841 aagagttgat tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata
     6901 aactgcgaat tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg
     6961 gcgtaaatga tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag
     7021 gtagtgaagt aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga
     7081 ttgcaaaagc cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt
     7141 tcttcttgcc aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc
     7201 gcccgctacc tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt
     7261 tatcttatgc actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag
     7321 atccaaagca aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac
     7381 tgatcatggt tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa
     7441 ccattcttct gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt
     7501 tgtccaagcc gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag
     7561 gtatccttgc tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg
     7621 gtacagtaag tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat
     7681 tcttagttgt agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa
     7741 cagctagaga atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc
     7801 aaacgattga gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga
     7861 gaatctagag ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga
     7921 tcggttagtt cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc
     7981 tgcaagaacg ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc
     8041 ggtttgatag aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt
     8101 ggttcattga tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac
     8161 aagtgtgcat attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct
     8221 tcaatcaata tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga
     8281 gatgtttttt ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg
     8341 aggacctcta aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg
     8401 ttgtaattcc aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga
     8461 taaatacctt ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg
     8521 ctgttggtat ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata
     8581 aagaaccaac atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg
     8641 tttcttaaaa attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg
     8701 ttatgagtga aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca
     8761 agaatacaag ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga
     8821 ttgattttag tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa
     8881 tcagggacag caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat
     8941 tgtttgaaaa aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg
     9001 attcctgctt gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga
     9061 aggtcagaaa aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt
     9121 atctttgtat gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg
     9181 gtaatgattt ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg
     9241 gagaaaataa acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga
     9301 gcatttactg atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa
     9361 aaataggtgg gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag
     9421 gcggattgaa catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac
     9481 tacttgtatt catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa
     9541 agagatggaa gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca
     9601 atttgtctcc agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa
     9661 aaagcaaaaa atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg
     9721 caagtgacca taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa
     9781 ttttgcgagg aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct
     9841 ctattgatct acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt
     9901 ttaaatcaac gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag
     9961 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc
    10021 ttattttcta agaaattact tctggaacac cgtttgttcg aaaaaagagc tgcgacaaga
    10081 attttgtccc agctcttttt tttttatttt cctaaataaa attcccgcat cgtttttgca
    10141 atcattttga tcaaaggatg atcttctgtg aagctagaag ataaataaat tgttcgtgtc
    10201 aagggcaaat ctttgatttc ccgcaagacg aaaggatcat ttttatcgta agaccaagaa
    10261 acttttggga aaaagctaac agctaatcct tgcttgacaa tagaacgtaa tgtcgcagga
    10321 tcatctgtcg tcgaactgat cgtcaattcg atacttttcg cttcaagtgc tgcatccagt
    10381 gtatgacgga aggcgttgct ctttgacagc atcaagatgg gggtgcgttg gatatcttct
    10441 aattgcagat gttctttttt agcaagagga tgtttcttag gaatggcgac ataaacaggt
    10501 tctttcatga taggaatatt ggtcatgcca ggcttcatct ccgaagtgat gtacagtaca
    10561 ggatcggtaa tatcatctgg taaatgatgt tgcatgatcg ttaattggat atcaggatgc
    10621 ttttccataa aaattcggac caagttcgga atagaaacag aggctacctc taaatacaat
    10681 atgacggaag taacgatctt ctcattgacc gaagacaata atttgattcc ttcttctaat
    10741 gtgtctaatg tcttgactgc atatggataa aattcttctg cataaacggt cgtctcaatg
    10801 tttctgccgc tatggtaaaa caaagggaca cctaattcgt tttccaaatg tttgatcgca
    10861 cggctcaagg caggttgaga aataaataat tctttggctg cttgggtaac attttttgtt
    10921 tcgaatactt ttttaaaata ctgcagatga agcaatttca taattatcca tcccatccat
    10981 acctttttgt tatggttttt tctttttttg gcatttttta ttatagcaga aattgactaa
    11041 gatagcagtg gaaaaagtaa gtgctgagga ggcgaggctc atgagtacag gtttcttatt
    11101 tcctggacaa ggcagtcaga agttagggat gttagcggat atccctgaaa tgtacatgga
    11161 acgtttagaa aatgtgacgg ggtatcggtt agttgataaa gaagaaaact atcaagatac
    11221 ggtttttatc cagctagcat tactgacaaa agcagtattt tatttagatg aaatggaaaa
    11281 aaacggtatt cagcctgata ttgcagcagg acattcgatt ggtgcttttt gtgcagctgt
    11341 tgcttgtcga tctttgagat ttgagaatgc agcggaacta gtctatcacc gcgcagtctt
    11401 aatgaaagaa gcttatccaa gcggttatgc aatgggcgta gtagtaggtg tgacgagaag
    11461 cgaagctgag gaaattgtag caaaaacatt tgatcctgac catccagttt atctgtcaaa
    11521 tgaaaattgt ccgatgcaac atacgttatc tgggagtatc gaaggtttat ccgttacgtt
    11581 ggcagaagcc aaacggcacc aagcgcaaac agcgaagctg ctgaaagtcc ctgtgccatc
    11641 tcactgtctc ttgatgaatg atacggtcaa gaaattcaag ccgtatatgc agtctgtcca
    11701 tctatctgat gctagatgtt tgtatataaa aaatacggat ggaagagcca ctcaggacgc
    11761 agaagagatt cgtaaagatt tgttggaaaa cctagctttc cctgtgcagt ggaaccaaat
    11821 gatggacttg gcaaaagaat taggaatgga tctgacgatt gaatttcccc ccggcaatac
    11881 tttgacaaag ctgattcata caaaatttgg tgtagataaa gggatccgta cgatcaatct
    11941 agaccaacat ggtattgatg acgccacgtt tctatatcaa aaatggagat gactaattat
    12001 ggaaaaacca aaccgcaact gggcttctaa acgcgaaaaa aaagcgcagc gattggaaaa
    12061 aatcagcaac ttgctcaatg gcaagtatgt agaaactgaa aaaatcgtag aagtaatgga
    12121 aaaactgatt gctcccggcg acaaagttgt attagaaggc gacaatcaaa aacaagcaag
    12181 ttttctttca gaaagtcttg aaaaagtaga tgcagaaaaa gtgaatgatc tgcatttgat
    12241 catgtctagt atctcacgac cagaacattt gaatattttt gaaaaaggaa tcgcaagcaa
    12301 gatcgatttc tcttatgctg gtgcacaaag cttgcgtgtc gcccaaatga ttgaagacgg
    12361 cacaatgaaa ttaggtgata tccatactta tttggaactt tatggacgct tattcatcga
    12421 tttgattcca aatgttgttt tagtagctgc agataaagca gataaagacg gcaacttgta
    12481 cacaggtttc aatacggaag aaacaccaac aatcatcgaa gctgcagctt tcaaagatgg
    12541 tatcgtgatc gttcaagtta acgaagttgt cgattcttta ccacgtgtag atatccctgg
    12601 tgactgggta gacgttgtcg tgaaagcaga taaaccttat caattagaag ctttattcac
    12661 acgagatcct caaaacatca cagaattgca aattctaatg gcaatgatgg caatcaaagg
    12721 aatctacgca gaacacggcg tacaatcttt gaatcacggt attggattca atacagcagc
    12781 tatcgaattg ttactgccaa cttatggtga atcactaggg ttaaaaggga aaatcgcgaa
    12841 gaactgggta ttgaacccac atccaacgat gattccagcc atcgaatcag gttgggttga
    12901 aagcatccat agttttggtg gcgaagttgg tatggaaaaa tatattgctg ctcgcccaga
    12961 tgtctttttt gtcggtaaag acggcacgat gcgcagtaac cgaacattat cacaagctgc
    13021 aggacaatat gccatcgata tgtttatcgg ttcaaccttg caattagatt acgaaggaaa
    13081 ctcttctacc gtaacaaaag gacgtttatc tggttttggc ggtgcgccta acatgggaca
    13141 taatcctggc ggtcgtcgtc attctacacc agcatggcaa tcacttcgcg acaatgatga
    13201 tccattaggg aaaggccaaa aattagttgt tcaaatggtt gaaacatatg gttcaaataa
    13261 aaaaccagtc tttgtcgaag aattagatgc actagctgtt caaaaacaag caggcttagc
    13321 caatgcacca gtgatgattt atagcgaaga tactacacat atcgtgacag aagaaggaat
    13381 tgcttatttg tacaaagcaa aaacaaaaga agaacgacaa gcagcaattg cagcaattgg
    13441 cggtgttaca cctcttggaa tgactagcac gaaagaatca ctagatagct tgcgtgcagc
    13501 cggcatcgtg aaattaccag aagatctagg catcaaacgt agtgaagcga aacgttcatt
    13561 attagctgca caaaccatcg atgatttgat cgaatggtca gatggcctgt atgaaccacc
    13621 aatgaaattc agaacttggt cataaaattg gcgagaaaaa ccttttgtcg ccatttaccc
    13681 agatggaaga agaaagacga ataagcagaa cgtgtatgct ctgttttaca aaaaggggag
    13741 agatccatgg aacaattagc ttataaatat ccaacgaaac agccgatcaa taagaaaatc
    13801 catgtaggag tggtcggttc aggagatttg gaaatcttga tgtttccaac agaaaaaaca
    13861 gaatctactg taaaaatttg tacaggaagc gatggcttcg gcgaagtatg gaacaatgta
    13921 ttaacgagat tttttgatcg ttatccaatt tcagcagata tcgtgatcaa tgacttcggt
    13981 gctacaccag gagttgtgta cctacgattg acgcaagcaa tggaggaact ttcagatgaa
    14041 aaatagtttt gtagaattaa acgccagaga acgagtgaac gctctacttg acaatggacg
    14101 tgaattgatt gatccattcg atcaaatcat cgcgccaagt ttaccagcac aaggaatcgt
    14161 ccctgaaagt gatgacggga tcgttgtcag ccgtggcacg atcaatggca aacaagcagt
    14221 agtaatcgct atggaaggaa aattccaagg tggcggtatc ggtgaagttt ccggtgcaaa
    14281 aatcgttgct gcacttgaac atgtgttaga agaaaataaa aaaggaaatg agatctttcc
    14341 agtcattgta ctagatacag gtggtgttcg tttgcaagaa gcaaactacg gactgttatc
    14401 tatttcagaa atcggaaatg taatcattgc tctaaaacaa tatgtaccag tcatcggttt
    14461 gattccagga cgtgtaggat catttggcgg tatgtcgatt acttcagcat taatgagtta
    14521 tttaatcgca acaaaaaaag cgagagtcgg tttgaatgga ccagaagtta ttgaacaaga
    14581 agcaggcgtt cgagaatttg attctagtga caaagacttg atttggaata cattaggatc
    14641 tcgtcaacgg gtagaatcaa aaatcattga tgaattagtg actgatagtg tagaagcaat
    14701 caaagctgct gtcatcgcag caatggacga acgaaaagac acgcatcgca gtgaaagctc
    14761 agcattttat ttatcattac ttgattcttt agatctttca aaaccaatgg ccattgacga
    14821 ttacaatgaa gcaattgcta accatcaagc aaaatcaatt gacttaccag aagtagcaga
    14881 aggaaatgaa ccagctgcag caagtgttgg ttacgattgg tttgcggcac taactggcat
    14941 gaaaaatcca acttcttcta ttagtactgt gtattatggt aaatcaagta aattcataga
    15001 agaagccatc gtgacatcaa tcgtacctga tcctaaaaac ccgatgtacc gtgtacgaaa
    15061 cggtgaagtt ggtttagttg aaggtttccg catgggacaa attttggatc acgtttacga
    15121 agaagataaa gacaaaaaag tgaaacggcc aatcatcgtt gtgatcgatg taccaagcca
    15181 agcatacggc tataacgaag aattgatcgg tatccatgtg gctttggcta atagtgctgc
    15241 tgcttatgca aaactccgtc aagctggaca tccagttatt ggattgattg caggaaatgc
    15301 tatttcaggt gcatttttag cacatggatt acaatcaagc cgcttgattg ctttaaacaa
    15361 taaagaaatt accgtccaag cgatgtctaa agaaagtgct gcacgtgtga caaaacgttc
    15421 ggttgctgaa atcgaagcag ctgctgaaaa agttccagct atcgcttacg atatcaataa
    15481 ttatacaaaa ctaggagcga tctatcattt cctagaagat atcaccgacc aagctgcttc
    15541 agaacaaaat gtcagcaaag tcatcgaagc aatcaatgaa gccattcaag atgtccgcaa
    15601 tacaaatgac acgatgctga caaatcgata cacaaacgaa acagccaaaa cattcggacg
    15661 tgtcgaaaca aataaagtcc gtgccaaaat ggacgaagaa tgggatgcat aaaaaatgat
    15721 tcaagcacat gatattgtcc tttttgataa acatgaaata gaagggttca ccttgcctga
    15781 atgggtcgtt ctaactgaaa agatgttcgg aacagttcgt cgtgcgtcga cggatcacgc
    15841 agaaatacta ccgattggtt tacgtggacc tgcccgcaat cagagatggt caacagaaat
    15901 ctcaaaacat gccgtcaaac aagtcattca tccgtgggac atcatcgaac aagaatcgta
    15961 tagacaagag aaaatcatgg attatcctgt ttatcagcaa tttcatgctg ctagaaacat
    16021 attatcagga tgtaaatggg gaatcggcgg cagcttagga tttgaattat cgactgggat
    16081 tccggcagta aaagaaacca gtgattttga tttgctgctg tacgcaaatt caccgattga
    16141 attgccaatt caggtaattc aatcgcatcc tgcttttttt gagcaattcg atacccaagt
    16201 agtgactcgg caaggcggtt tttcactaaa agagtatttg agagagccag agaaaaaaat
    16261 tctattgaaa acagtcgaag gacctaaatt aaccaaagaa ctttggtaat aaagaaaaag
    16321 agagagaaag agatgtttga aaacaaaaaa atcaaaatca acgtaagtga cttccagaaa
    16381 gtatcagctg taacggctgt tatgatgcaa acgatattga gctttgcttt ggcaaacagc
    16441 cacagcgcaa caaatacagc ttttcttggt accttttacg tcatcgttaa atacacagca
    16501 ccaatgttta tttttgcaat tgtttataat atggtaaaaa caagtcagca tttgtcttat
    16561 tgggaatttt tgaaagaaaa gttttttgaa ctagtcgttc cgtatcttct atggacgacg
    16621 gcttatcttt ggctatttcc agcagtccaa caacaaactc cgtatacgga tactgcaagc
    16681 tttttactga aatatgtcac aggtgatgga gcgcctcatt tatggtatac cgttatgatg
    16741 ctgcagatcc aattgttcat gccgtttttt gtttggctcg gttacaaagt atttgctaat
    16801 aaaaaatatg tatggccagt attgattggt tcaacgattt tatatgtcgc atggtatctg
    16861 ttctatcaaa cacaagtatt gactgggcct tatcatgaca gctggtactt attagatcgg
    16921 tttgttgcta gtttcatgat ttatggggta gccgcatttg tttatcacga aaaagtctat
    16981 caatatttgg acagagtacg ttatcttttc ttacctgttg ccttagttat cgcgttcttt
    17041 tctgtccgtt cattgctcgc acatccagga gacttgagct ttgcgaatgc accttacttg
    17101 aatacgatcc aaagtttata cagtttagtc atcatctttg ctgtcttcat aggcgcttcc
    17161 aaaatgattg taaacgattc gccaaaattg ccattgttca aatggttgtc tgtttatgct
    17221 tatcgtacgt atttagccaa tgtatttgtt ttccaagtac tgttattatt gtttaaagat
    17281 agttggctgc agttaccttc aggagtcatg attcttgtag cttatctaat gacggcaagt
    17341 tgtgcatttg ctttaagctg gttacttcat atcatttggg tagctatcaa aaaaggtttc
    17401 agcaagtaat agtaggaaga aggagtgagt gtcatgataa atgacgaacg aaaaaaagca
    17461 gtgactcagc aactcgctgc gttggtagag gaagcattga tcgcagaagt aacattaagt
    17521 ccaaaacctg gattagtgga tgcactggat gcgggtgcac attccgatat ggactatgct
    17581 ttatttctta agtctgctaa gacgctaact cctttttttg aagaaatggc tcaaatagca
    17641 tggcatcatg ctattgatca ggagttaaga gaaagaattg ctgagattgg gcgaaaagcg
    17701 gaaaaggcaa tgctgattgc tacaaatggc atcaataccc ataaaggagc gatttgggca
    17761 atggggctat tgatcagtgt cattgcacaa aaaatcagta tggaacagcc tttttcttta
    17821 tcgaaaatac ttgatgatgt cagcaagctt gctgcttttc ctgatacaaa atatcgtcat
    17881 aaaaaaacca ctcatggtca tagggtgaag caaaaatatg gcgtaaatgg tgcatatgaa
    17941 gaagcgatat tagggtatcc gcatatcagg cttgcaattc aagcagctaa ggagctagat
    18001 aatgattcat atgtacagaa acaaatgcat atgttgttag ttttgatgag ttcgctagat
    18061 gacacatgcg tattgcatcg cagtgatcag aaaacgttag tggaaatgca aaaacttgct
    18121 catgctgcta gtcaaagtgc tttaccaaat cgtccattta cagcactgtt gcacttttgt
    18181 caggaaaacc aaatctctcc tggaggaagc gcagatctct tgtctgcaag cttgttcatg
    18241 ctgaagatag aggaattatg gactgccgac acatacattt gtccgatgtt actcaatgac
    18301 taaataagac aaaattgcta aaaaaacagt ttatgcatct ggttattgac tcttcctttt
    18361 agcaaggcta tcataaaaat agttttttgt aataaaagat aacgaggaag acaatgaaaa
    18421 aaatcaatta tttagcaatc attttgttag tgggcctatg tttgtttgct ctgccgaccc
    18481 aaggattcgc tgtcgcgggt ggtcatggtg gaggtacatc tagcggtcga agttcgggca
    18541 gaggttcatt tggtggtcgt tcatatgggg gcagttattt tggcagtcgt tcttacggca
    18601 gagattctta tgggtattat ggtccaggta caggtccttc tagttatgag caaacgggat
    18661 ttcttgcatt agctgttttg tcagtaggag gggtttctgc tttttctatc gtcaagaata
    18721 aaagacaaaa gaaaaggctg aaagcggaga tattgtcccg catgtcagga acggctaaag
    18781 aaaagaaagc acagtttctg gaaatccaaa agatattcat gaagatccaa tccgcttggg
    18841 aagaagaaac tcctgaaaaa gccagaagct gttatacaga caagctgttt gccgaacatc
    18901 agaaagttct gcaaaaaaac aaagcagacg gtgtaagaaa tcatactaaa aaaataacta
    18961 ttcaggaagt aggaaattat cgacagatcc acgaaaatag ttttgccatc cgaattgatt
    19021 tcagatgctg tgattattca gtcgatcgaa aagatgggcg attgatctca ggaagtaaga
    19081 cacaacgaca gtatttttct cagatatggt attttgattt ttctgagaaa gaaaaaagat
    19141 ggcaagccga ttttattcag ccgattatgc tggattgaca ggaaatattt atgaacggag
    19201 ttgtgacaaa agccttgtcg cagctccttt ttctgaataa acggtgtgcc agagatagcc
    19261 tatcggcaat aaggcacgat tttgtaaaat atgggaagcg atttttcaaa atcgcttctt
    19321 atcgctcaag gctgaacatc tctgtcacaa cctctccact aaatggtgtt taaaagcttg
    19381 catctgcgat aataagaaca accaaacgaa aaataggaag cactatttta gtttagttgt
    19441 tcttattact tgatgcagga cagctttttc acaacctctt ttttgtatag atgggcaaaa
    19501 tttttgtaat aataggcgta tgtctttttc gttgaggaga tgaaaaagca tatggcttat
    19561 tttttgtttg ctttgcttac tttctttcca cttcaagtgg cagaaatatc tactttttat
    19621 tatgtaagcg gaaccatata cttgaaatca gaaggagatc atttctatga aaacaagaac
    19681 aattgaaata cttcacagaa tcatgacagc ccaatatcca gtaaaagcaa aacatctagc
    19741 aagtgagttc aatgtcagtc agcggacgat ccgtcaggaa gttcttgaag cgaatatctg
    19801 gttgagcagt aaaaagcttc cggagatccg tacaattcgc aacaaaggtt ttttgttgaa
    19861 gctggaaaag gaaaagcaag ctcgattaga aactgaaata aaagatgtga aagacggctt
    19921 tttgaatcgg cgcgaacgta tttttgatct agttttagcc gttgcttaca gccgaatccc
    19981 gatccatcta aacaaaaaag aagaagaatt tcaaatctct aaaagtacat tagatgaaga
    20041 tatgagacgg ttacgagcaa cactggtcag ttatgggata gagatcgtca gttatggaaa
    20101 gcaaggactg gtttataaag gttcagaacg atctatccgt acgatgatct atgattttat
    20161 caatcagaat ctgggaatga ccgacttctt tatgaatcaa gatgcacaat taacaccggc
    20221 tcaaaaaatc tttctccatt attttccttt ggtggaaatc aaaaaaatac aggagatcta
    20281 ttccaataag ttgttgaaac aagaagatga tatttataaa aaacaaatcc tgctttttac
    20341 attgatctgg atccagcgaa tcaggaaaca tgaatttatc tctgccatta attggaaaaa
    20401 tacagaaata aaagacagta gcttttctgc atttattgac cacatgatcg aggaatttca
    20461 attggacaat gtcccgcaag tagaaagaaa ttatcttcgt ttcactattg agacgttcaa
    20521 tgctagagat atctccaatt caatcgagtg ggtacaagct caattactaa cgatccaatt
    20581 gatccaattt gtagagaacg agacacacgt acctttccat ctaaaggaag aagctttatt
    20641 cgaaaatctc tataagcaca tggctgcact gattgtaaga atcaagaatc atatccaagt
    20701 catgaatcca ttgaaagaaa acatccgaac aaattatttc ccaatctacc gcgcagtcac
    20761 tcattttgtt cctacgatcg aagaagtaat tggtggcaag atactggagg acgagatagc
    20821 ttttcttgtc atccactttt cgacgatcgc cagtgtaatc aagcgagact tgaattatgt
    20881 ttataaatca gtcgttgtct gtaaccatgg gatggctact ggaaatctgt tagcagagaa
    20941 tctgaaagaa aagtttcctc agatcgagat tactgcagtt ttaagttcaa aagaaatcga
    21001 attagtagat aaattggatg tcaatctggt tttcagcacc tttcaattga attatcgaaa
    21061 taagccgctt ttagttgtcg atccgatttt aactgagacg aatatcttgg ttatagaaga
    21121 ctttttgaaa agaaatgaag aactacagcg gttagagcct aacagtcatg aatcgacgga
    21181 gttattcact caaataatag atgtcatgaa agaaagtggt ggccgagtag atcgaatcat
    21241 ctatggcaag ttagaaaaat tattcgaaac aaacaattta gaaatcaaca aaagggagat
    21301 ccagcctatg ttaaaagata ttttgacgga cagcaatatc atgatcaaag aaaaagccac
    21361 tacttgggag gaatcgattg aactagctgc tagaccattg gttaaagaaa acattgtcga
    21421 agagcgctat gtcgaagcga tgattcgtgc agtgaatgat tacggaccgt atatcgtgat
    21481 tggcaaacac atggcactag cccatgcccg accagaagat ggggtcaata aattaggagt
    21541 cagcgtagct acgattgagc aaccaatcga tttcggtaat cctgaaatgg atccagtaaa
    21601 gattattttt tgtttggcag cagtagactc ctactcgcat ctgaacatca tgaaagaact
    21661 aattgaattg atcaacaacg aagagaagtt ggatcgattg attgaatgca caaatatcga
    21721 agatttcaaa caactgcttt tttcagtagt tgagaaataa taaatgatac gggggagaac
    21781 aaagatgaac aaattgaaca ttttattcgt atgcggtgca ggtttaggaa gtagttttgc
    21841 agcacagatg tctgctgagg atgttttgaa caaattagga gtagaagcca aactggacca
    21901 ttgtgatatt tcttccgcag tttccatgaa accagatgtg atcatcacag cagaaaattt
    21961 tcgttcgcag ttcgaaaagt tcgcagttga tcctaaaacg gcgatgatct atttgaaaaa
    22021 tattgtttca aaaaatgaaa ttgaagaaaa aatcacccca gttctgcaag ataagggagt
    22081 tatctaaata caaaaagaaa agagggtaaa aa
//
LOCUS       DAZKMZ010000042        22076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000042 DAZKMZ010000000
VERSION     DAZKMZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.042"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8M5_001671"
     CDS             complement(270..1766)
                     /gene="lysS"
                     /locus_tag="ACG8M5_001671"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0358926.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8M5_001672"
     CDS             complement(1877..2881)
                     /gene="dusB"
                     /locus_tag="ACG8M5_001672"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0358927.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8M5_001673"
     CDS             complement(2882..3769)
                     /gene="hslO"
                     /locus_tag="ACG8M5_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0358928.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8M5_001674"
     CDS             complement(3986..6097)
                     /gene="ftsH"
                     /locus_tag="ACG8M5_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0358929.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8M5_001675"
     CDS             complement(6187..6732)
                     /gene="hpt"
                     /locus_tag="ACG8M5_001675"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0358930.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8M5_001676"
     CDS             complement(6732..8177)
                     /gene="tilS"
                     /locus_tag="ACG8M5_001676"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0358931.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8297..8767)
                     /locus_tag="ACG8M5_001677"
     CDS             complement(8297..8767)
                     /locus_tag="ACG8M5_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0358932.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8813..9238)
                     /locus_tag="ACG8M5_001678"
     CDS             complement(8813..9238)
                     /locus_tag="ACG8M5_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0358933.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9305..9574)
                     /locus_tag="ACG8M5_001679"
     CDS             complement(9305..9574)
                     /locus_tag="ACG8M5_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0358934.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9585..11180)
                     /locus_tag="ACG8M5_001680"
     CDS             complement(9585..11180)
                     /locus_tag="ACG8M5_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0358935.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8M5_001681"
     CDS             complement(11195..14716)
                     /gene="mfd"
                     /locus_tag="ACG8M5_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0358936.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8M5_001682"
     CDS             complement(14733..15293)
                     /gene="pth"
                     /locus_tag="ACG8M5_001682"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0358937.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15646..16620
                     /locus_tag="ACG8M5_001683"
     CDS             15646..16620
                     /locus_tag="ACG8M5_001683"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0358938.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16790..18190
                     /locus_tag="ACG8M5_001684"
     CDS             16790..18190
                     /locus_tag="ACG8M5_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0358939.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18268..18990)
                     /locus_tag="ACG8M5_001685"
     CDS             complement(18268..18990)
                     /locus_tag="ACG8M5_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0358940.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19069..19575)
                     /locus_tag="ACG8M5_001686"
     CDS             complement(19069..19575)
                     /locus_tag="ACG8M5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0358941.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19698..21497
                     /locus_tag="ACG8M5_001687"
     CDS             19698..21497
                     /locus_tag="ACG8M5_001687"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0358942.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21565..>22076)
                     /locus_tag="ACG8M5_001688"
     CDS             complement(21565..>22076)
                     /locus_tag="ACG8M5_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0358943.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6111 a         4828 c         3993 g         7144 t
ORIGIN      
        1 ttttgttatg tttttgaata aatattttct tctgtctttt ggacaagtaa taatatacca
       61 actaaattta gcttggtcaa cgaatttttt gtattttttc aagttttttt caataaacga
      121 ttattcataa cttttctttc tttatcgttc gttttcgtct tctttaatgt ctagaatagt
      181 cggcattagc ttttgaactt tcgttattat ttattccttt ctacacagca aaagctgcct
      241 aaggaattcc ttagacagct tttattcgat tatctcattg ttgggaatag caaaacatcc
      301 cgaattgatt gtgcatcagt tagcaacatt actaatctgt cgatcccgat gcctagacca
      361 ccagttggcg gcataccgta ttctaaggct tccaagaagt cttcgtctac accgtgagct
      421 tcgtcatttc ctagttcacg ttctttttct tgcgcttcga atctttctct ttgatcaatt
      481 ggatcgttca gttctgtaaa ggcattagca aattcgcgcc ccactatgaa tagttcgaat
      541 cgatctgtga atcgaggatc ttctggattt ttcttcgcta aaggcgaaac ttcgactgga
      601 tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca
      661 ttgataacat gtccgacacc catagcttcg gttacttcta cattgtgttt ttcggctaaa
      721 gctaatgctt cttccaatgt catttcttgc cagaagtcta caccagtttg ttctttgatt
      781 gcatctacca tatggatgcg tttaaagtca ctttctaagt cgacagcttg tccatcatat
      841 gtgatttttg ctgttcctag tactttttca gctgcgttac ggataatacc ttctgtcaga
      901 ttcattacat cattgaaatc agtatatgct gtgtatgctt ctaacatcgt aaattctgga
      961 ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc cgatttcata aactttttcc
     1021 attccgccaa caattagtcg ttttaaatga agttctagcg caatacgcaa atataaatca
     1081 atgtccagtg cattatgatg cgtgataaat gggcgtgcgg cagctccacc tgcctcattg
     1141 tgcaatactg gtgtttctac ttcaatatag ccattgccat ctaaatagcg acggatttcg
     1201 ctgataattt ggcttcgttt cataaatcgg tcaaagcttt cgcgattact gattaaatcc
     1261 aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc catggtattt atctggcagt
     1321 ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg
     1381 tctgttttca tgatttgtcc agttacacca aagaaatctc caaggtctgc atgtttgaat
     1441 acttcatagg cctcatcacc tacttggtct tttcttacat aaatttgaat ttggccttca
     1501 cgatcttgta gatgcgcaaa tccagctttc ccttttcctc ggcgagtcat catacgacca
     1561 gctacactag cggtcaatcc catttctgct agttcttctt ttgttcttgg atcaaatagt
     1621 tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca
     1681 tcttcacgca attgttccat tttttgacga cgtacaagca tttgatcatt taaatcttct
     1741 acttgatgtt gttgttcctg tgtcacaaaa gttcctccat tctcatgtta atctctttcc
     1801 tataatgcca atgaaacaca aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc
     1861 acgaaaaact tagaaatcac aacgtttgtt tcaatagtac tttttccgct ttttctacaa
     1921 attgatccag caggtcattg atttcttgct gtttttctgt ctgattgatt gctactttca
     1981 ctttcgctgc tcgaggaatc ccttttaaat aataagccgc atgtgttctg aattctctac
     2041 aagctacttt ttctcctttg agatctgcta agcgttgtaa atgaagttta gctgtagcaa
     2101 ttttttcggc tggtgttggt tcaggaatca gttcccctgt ttcgagataa tgctgtgtgc
     2161 ggtgaatcat ccatggattg cccagcgctg cacgaccaat catcacaccg tctgcaccga
     2221 catagtcaag catccatttt gcatcttctg gcgttttgac atccccgttc cccataaatg
     2281 gaatcgtcaa atgttttttc acttctttca atacttccca gtccgctttt ccttcataca
     2341 tctgaacccg tgtacgtcca tgcatagcga tggcacttgc tccagctgat tgtgctgcta
     2401 atgcattttc tacagcaaag acgtgctctt catcccaacc aatacgcatc ttgattgtta
     2461 ctgggatatt tactgctgaa gaaacagctt ggaccatttc gtatactttc tcaggatcta
     2521 aaagccattt tgctcctgct tctgctttga ttactttatt tactggacag cccatattga
     2581 tatctattat atcggcagct gtgttttctt cgacaaattg agcagcttca actagactat
     2641 ctttatttcc gccaaatatc tgtaaactta atggatgttc tgtttcttca atgtggagca
     2701 tctctaaagt ttttttgttt ctgaaatgaa taccttggtc actgatcatt tcacatacga
     2761 ctaagcccgc accaaattct ttgacagtga ctcgaaatgc tgaatttgtt attccagcca
     2821 tcggtgcaac aacgacacga ttagggattt ccacattgcc aattttccat gtgtgttcca
     2881 cttaaatccc tcctaaggct tctttcttca atccatctaa ttcttcttcg ctatacttgt
     2941 atttgttatt acagaaagaa caaaccgctt ctgcaccgtg atcttcgtct atcattgctt
     3001 ggatttcttc tgcacctaac gcgataattg cttctgcaaa tttttctttt gaacagtcac
     3061 atttgaattg aacaggcatt ttttccaaaa tgttcaattc ttctgtacca agcaatcctt
     3121 tcaatatttc ttctggtgtc tgtccttcat ctaagagggt agagattctt ggtgtcgttt
     3181 ttaattgttc ttcgatcttg ctgattgttt cttcatcggc acctggcatg atttggatca
     3241 tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac caatacactc agtccaacag
     3301 ctgaagggat ttgttctgag atagcaagat agtaagtaaa atcttcagcg atttctccag
     3361 aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa
     3421 acattccttg tgttccaact gctccccgca catcgatttt accttcacta tttagaggaa
     3481 gactgagatg cggatttttg atgtaacctt tcacatctcc ctttccattt gcatctacta
     3541 caattcctgt tgctggacca tcgccttgga ttcgaacagt tagtttgtct tctcctttga
     3601 gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc caaagcagct gaggaggtgt
     3661 gccaagtatc atgacgtctt tgtgcctccg ctacggtatt tgttgcttga acggcgtatg
     3721 ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata atctttcatt tgttatgtac
     3781 tcctttttcc gtttttaaac tcatagtgaa tcatactagc aactactgct tttttcaact
     3841 ttggaatgct tcttttaaaa ataattcaga atcatctaga aaacaggaca tcaccctatt
     3901 tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat
     3961 atcagatcaa ttgacctata tttttttatt tgtattgatc gtctgaatga ttatcttctg
     4021 attgttgaga atctgaatca tgttctgctt tattttcctc attcagttca tgttttgctt
     4081 cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt
     4141 ttgcttcttc aaaagttttc gcttcttttt cgcttggata atcatttgac tcagtacctt
     4201 caggcatcac accgtgttca aacaatgact tgattgcttt ggcatccaat gtttcgaatt
     4261 ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg agcttcgatg atttctcgag
     4321 ctttatcatg agcttccatc aagatttttc gtacttcttg atcaatctcg aaagcgactt
     4381 gttccgagta agcttttgtt tgaccatagt cgcggccaac aaacacttga tggtttcctt
     4441 catattgaac aggacctaaa cgatcactca ttccatattc tgttaccatg cttcgtgcta
     4501 gagctgtcgc ttgctcaaag tcattggaag caccagttga ttggacattg aagatgattt
     4561 cttcagctgt acgtccacca agtaagccta cgatttgttc aaacatatct tctttcgtca
     4621 tcaagaactg atcttctttt ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga
     4681 tgatcgttac tttgtggacg acccgagcgc gactcaatac taatccgaca atcgtatgac
     4741 cagcttcgtg ataagcaacc atttcgcgtt cttttttgct gattacgcga tcttttttag
     4801 ctggacctgc aatcacgcga tcttccgctt catcgatatc agaagcatcg attttctttt
     4861 tatttcttct agcagctact aacgctgctt cattcaagac attttctaaa tctgctccgg
     4921 caaaacctgg tgtctgttga gccacaactt tcaagtcaac gtcatctgcc atcggtttat
     4981 tccgtgcatg gactcgcaag attgcttcac gaccttttac atctggacgt ccaactaaaa
     5041 tctggcggtc aaaacggcct ggacgtagta acgctgggtc tagtacatca gaacggttgg
     5101 ttgcagcaat gacgattact ccttcatttc cgtcgaagcc atccatttct acaagcaatt
     5161 ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat accggcacca cgttgacgac
     5221 ctaccgcatc aatttcatca atgaaaatga tagctggtgc gtttttcttc gctgtttcaa
     5281 acaaatcacg aacacgactt gctccaacac cgacaaacat ttctacgaaa tcagaacccg
     5341 agatcgagta aaacggtact ccagcttctc cggcaacagc ttttgctaat aaggttttac
     5401 ctgttcctgg aggtccttct aaaagtacac ctgccgggat acgggcacct aattcaacaa
     5461 atcggcgagg atctttcaga aattcaacaa cttcaactag ttcttgtttt tcttcttccg
     5521 cacccgctac atcagaaaaa cgtacgcgat tagctttttt gtctgcttct ttggcttttg
     5581 atttaccaaa gttcatgaca cggccgccac cgccaccgcc gccgccttgt tgtcccatca
     5641 tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa gctaagcaat agggaaaccc
     5701 atataccgct gcttgattct tcttttactg ttgtagcaac tttgttttct tttgccagtt
     5761 ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag
     5821 tcgtatctgt tgaacctaag atagctaacc caccgctgtt agcgacggtc tgctgttctt
     5881 tgtattcacc agtgatctta tatacaccat ttgcaggctg cacttcaaat tctttgatct
     5941 tgcctgcttg tagttgttct gtgaattttg agtactcgat atctgacgat cgctggccgt
     6001 tattaccaaa aatgaaataa acgaccatta ccatcgctaa gattacgagg acataataca
     6061 aggcattttt catcatgccg ttgttctttt tgttcatagt tgccctccct aagcgtcaat
     6121 ttaatttaac agttttttat caatcctgac cattctattg taccataatt taattgagat
     6181 acagcattaa tttgattgat aaacactagg tttcaataca ccgatgtacg gaaggttacg
     6241 atatgcttct gcataatcca agccataacc gaccacaaat tcatttggta cgttgaagcc
     6301 aacataatct gcttcgatat ttactactct tccttcaggt ttgtcaagta gtgtaacaat
     6361 tttgactgat tttgctttgc ggtatttgaa aagatccact aaataagcaa gtgttcttcc
     6421 gctgtcaatg atatcttcaa cgatcaacag atcgcgacct tctacattcg tatctaagtc
     6481 tttgacgatt tttacttccc ctgacgagac tgttgcattc ccgtagcttg aaacatccat
     6541 gaaatccaat tccaaatacg tatcgataga acgaacgata tctgccatga atggtaccgc
     6601 tccttttaag acacctacta ctaggggatt ggtgttttgg taaatttctg ttaactcttt
     6661 gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag atcaaaattc tctcaatatc
     6721 cttttccaac atcaggttct ccttccaatt gcttcttttt gatatttgta aagaagtatg
     6781 tagtgtattt tatcagtttc ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag
     6841 ctatatatat ttcttttggc atctgtaatg atccaagcat gctgcctttg tgaattaggt
     6901 actttctgat cgataaaata tctagatact ttttttgtga gattctctgt taatttaatg
     6961 cggtctcctg cttttctttt ttcgacatac agttgcacat cagcaggaat ccaaatgtca
     7021 tgagaaaata gagaccatga atctgttgct ttgatgtccc ctgcattttc aggtttgacc
     7081 agaccaatcc attctgtttc attcaaatat attccttgtt ctggaatcag caacaagcgc
     7141 tggtcttctg tctggctagt tgcttgtttt tcttttttag agagtacaac aaaatgatac
     7201 tctcttttca gtatccaatt attgccaata ctgagttgcc attggctttt tttctgttgc
     7261 cactggtcta aaatcgactc catttgttgt tctttcaaaa taattcctgt ttttgagaat
     7321 gctttgtcga aaaaggagta aaaaaaatag tatcgttcag catctgtcat ttcttcaatc
     7381 atttctgcag aaaatcgaaa ttgttcctcc cactgttcga cttccttttc gatcaactgt
     7441 cccatatatt tttgaatgac ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa
     7501 tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa ctaccaaatg acgaagccga
     7561 tttctctgga tatctaacaa ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc
     7621 gctgcataag tatataggtc ttctttgcta aaactcagca acggacgaat cagtctgccc
     7681 gtagcaaatg gttgtacttc tttgattcca gaaaaagtat ttaactgtcc cccacgaacg
     7741 attttcatta acaccgtctc gatttgatca tctccgtgat gagcagtcat caacgtgtca
     7801 tattgtcctt cttccatcac gcgagtgaat tcttgataac gaaagttacg agcttctgtt
     7861 tctatggcgg atttggctgg atcttcccaa atacttacat ataaagggag attcttctct
     7921 tcacaatatg agcgaagata ggcagcttct tctgctgatt cttctcttaa ttggtgattg
     7981 atgtggacga tacctaaaga aaaaccgatt tggcgttgca cttgtaaaag taaatggagc
     8041 aaaaccatcg aatcaacacc acctgatacc gctgctaaaa ttctcgatcc ttgttgccag
     8101 aatccttgtt cattcccgtg ccgaataaat gactgctgta atagcggtgc ttttttcccg
     8161 cctttttcaa cgttcatcct ttccacaccc tttcttccta aaaaaaagag agcgggaaaa
     8221 cgtctaaaac gacaattaac ctgccctctg ttgatactat gacccaaata gtcgcactat
     8281 cattatttgc ggctttttag ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt
     8341 ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa aaatgaactc atcaatgaat
     8401 cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa agattttttc tgtgctggac
     8461 gatttcggtt gtcacgggga tctttttgtt gatatttttt aggacggctt tcttctccag
     8521 attcagcagg ttgttcctgc gctttccgga tagatagtcc gatttttcca tcatcattta
     8581 ctgaaataac tttaactgtt acttcatctc ctacacttaa aacatcatgg atgtctttta
     8641 catagccatt tgacacttcg ctaatatgaa ctagacccgt ttttccagca cctagatcaa
     8701 taaacgcacc aaaatttgtg attcctgata cttttccttg cagttttgct cctacctcga
     8761 ttgacataaa aaaaatgttc ctcctaatta tttccctttt ttatattcct gcttattttg
     8821 cagttgtaga actagttgat tgatctgttt catttgtcga gctgtctgta tttgattcta
     8881 atataggata aactttttca ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag
     8941 ctacatactc atcatctttc agcaatgaaa catcttggtg cagctgatca acgtctgcgt
     9001 tgacttcttt catttcagta tttgcttcga ctctcaattc gttcaattga tgcatccttt
     9061 gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa
     9121 ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg ttgttgctga aattcagcat
     9181 actttttctt tgcatattcc gtatctagcg cagcgatttt attgcttttt ttcgtcactt
     9241 ggattcgctc caatcttttt gattgtcttt ttcattatac caatatcgtg aaagcttgtc
     9301 taatctattt ctctagtgtt tctgtgcgat tttcagaaat aatttcgtac attttttgtg
     9361 catcttcttt tttggttgat tcatgtaatt ccgtaatttt cacttccaac gttttgttgc
     9421 caaactggac tttcaaaata tcgcctactt tgacatcact ggaagatttc gccaatttac
     9481 cgttgatttg aattcttcct ttatcagcta cttcttttgc tactgatcgt cttttgatga
     9541 ttcgggaaat ttttaaaaat ttatctaatc gcatatctta ttcctcactt tttaattttt
     9601 ttatttaatc gcaggatttt cttaccaaaa ggcaacatta gccattctcg tactgtaaac
     9661 agacgaagac gaatcactgc ataaatgaac acagaagccc cgatcccaac tcctatcacg
     9721 ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa atactatatt caatacacca
     9781 taatacagca gcagtactac tgtcatacaa agaaggctga acatcaactt ccgtccaaaa
     9841 tgccgttctt tccaaaaagc atttagctct tttgtttcaa ttcgaacaaa ataccaaagc
     9901 gtcacaccta atcctaaaag ggtagacagg cttgcacctg ctgttcctaa taaaccggtc
     9961 agaaaagaag tagtcaatcc ttttaccaat agtccccatc ctgcgccttt taaagaaggg
    10021 cgaaacgagt tcttgctttg agcaatactt tgataagctt ggatgactgc agtgaaagca
    10081 atggaaaaaa caaataatac aagcgctgca tttcccgcat aatctttaaa caacgcataa
    10141 ttgatataag ggagcagcaa ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc
    10201 aatctaagat agattttggc tgtctgtaaa aactgacgat agatccggtt ggtcaaatga
    10261 cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg caatcactaa acctaattgt
    10321 actaaaggct gacctcgatc atagacccct tttgctatct tcgcaccgta ttctgacagt
    10381 ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga tcagctggaa aaagataagc
    10441 aatccactat aaatcgaaac cagtccgcct tctaccagaa aacgccggat caagggcttt
    10501 gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt
    10561 tcatagtagt gaaggatccc atatgcgcat aaaccgccaa aaacagctcc actcattgct
    10621 gccgtccctg tttggtacac cgtccatcca agctttctaa aagcaaacgc actacagaca
    10681 atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa cagcagttgg ttccatcaag
    10741 aatcttcctt ggaaattccc tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag
    10801 gaaactactt ggatcagcgg tttcaattgt atgtctccca tcatccaagc aatccagctg
    10861 ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc aaaatactaa gggataaaga
    10921 ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg
    10981 aactgcggaa gaccggataa agctaaagtc attgctatcc cataaattgg ataaacttgc
    11041 tgataaacat aaaaaccttc gtcgccgact aagttttgga ggggaattcg gtaaagggca
    11101 cttaatactt ttgctatgaa agaagcaatc gtcaatacaa atgctccttg catgacgcgc
    11161 cgcatttctt tatgatccaa atcattgctc cttcctacgc tgacagatat ttttgctcac
    11221 gcaacgcttt gacaaacttt tggatttcct gaagccatac tgcttcttcc atttggttgg
    11281 ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc tagactggct tttagttttg
    11341 tcgctgtcag tgcttcgaat aactgttcca cactatagcg ttttgttcca actttactta
    11401 gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc taggagcgct cgatcgccat
    11461 ccattttgat ctgcccaatt gtcaagagat gtgctacttc ttctggatat tctccaaaac
    11521 ggtcgagcag atcatcttct agttcatcta gcatttcttg cgaatcaagt tcacgaattc
    11581 gtttatagat ctcgattttc tgccgttgat ctgccacgta tgtttcaggt agatacgcat
    11641 cgacaccgag gtcgatctca acagtggtct tctccacttg actattcttt ccttgtttac
    11701 gagaaacagc ttcgcttagc atttgagaat acatatcgaa accgactgca tcaataaatc
    11761 catgctgctg agcacctaag agattacctg caccgcgaat ggaaagatcc cgcatcgcaa
    11821 tcttgaatcc agatcccagt tctgtaaaat ctttgattgc ctgaagacgt ttttcactga
    11881 cttcgttaag gatcttctgc tgctcataca taaagtaagc atatgcgaca cggctgcttc
    11941 ttcctacccg ccctcgcaat tggtataacg tggataatcc catataatca gcgttctcaa
    12001 cgaacaacgt atttgcgttc gggatatcta ctcctgtttc aatgatggtt gtagtgacaa
    12061 gaacatcata ttgaccttca ataaattcaa aaagcgtatt ttctaattgc gcctctgtca
    12121 tttgcccatg agcataaccg atccgtgctt ccggaacgag ttcttggatt tcttctactt
    12181 tttgttcgat cgtttctacc cgattatata aatagaatac ttgtccacct cgtcccattt
    12241 ctcgatggat tgcttcgcga atagccccag gatttttttc cataacatac gtttggatcg
    12301 gataacgatt cgcaggcggt gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca
    12361 tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa aacatccact tgtgcacgta
    12421 actgtttcaa tcgttcctta tgttttacac caaaacgttg ttcttcgtca atgatcagta
    12481 gacccaaatc gctaaattca atatcttttg acaaaattcg gtgggttcct acaacaatat
    12541 caacttgacc tgtacgcagt tgttcgatgg tttctttttg ttgctttctt gttctgaaac
    12601 gacttaatag cccgatattt acggggaaac cttcaaatct ttccagcatc gtttcatagt
    12661 gctgctgtgc caagatcgtt gttggaacta aaaaggctac ttgtttgctt tcctttatcg
    12721 ctttaaaggc cgctcgaagc gctacttctg ttttcccata accaacatca cctacgagta
    12781 gtcgatccat tggtttttct ttttccatat cccgtttgat ctccgctgca cttctcaact
    12841 ggtcatcagt ttcagagtag gggaaggcat tttcgaattc tttttggtag gcgtcatcag
    12901 gtccaaaagc atatcctttt tctgactctc ttgcggcata taatttgatt agatcgtcag
    12961 cgatatcctc gatttttgaa gagacttttc gcttggtttt cgtccattca cttccgccta
    13021 gcttattgat gcgaggcgct ttggcttcag aggcgacata tttttggatc agattcagtt
    13081 gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt
    13141 gaacgccatc tacttctagc gtttccatcc caatgtattt cccgatacca tgattggcat
    13201 ggacaacgta atctcctgct tttaactcat tatagctttt caatcgctct gcgtttgtta
    13261 ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa aatttctttt tctgtgatag
    13321 ctactatttt ttcttgagga agttcaaaac cagcctgcaa agctccttca actaattgta
    13381 cctgtcctct gatcaatgca tttttttctg tttctactac taatatgtct tcatctcgaa
    13441 gcatttgttc tgctttgcgt attctgtcct ttgtcggaat aaagatcaca acagtctgtt
    13501 tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg catttgtccg aagaactgtt
    13561 gcatcgtccg atattgaaaa ttatggattg cttggaaacg aagatttccc atcccttttt
    13621 ggaagagtga gaaaaaggtt gtggtgaact tttccttctg cacgattttt tgtatatctg
    13681 ctgaaaatgt ctgctcagaa aacacccgca attcgctgat tttttgagtc tgccattctg
    13741 ccgcttccct ttcgatttcc cgatttgttt ccatgattcg ctgataatca tcaacgacta
    13801 gtaaactatt tgaagggaaa taatccaaga tcgtcacaga ttcttggtaa agaagatctg
    13861 agtagaattt tgcttcttct gtcgggacac ctgcttgcca gcttgaagtc agctgaccga
    13921 agtaatcttg caaaaactcg cggtctgctt cttctgtggt cgcagcaagc cgtttttcta
    13981 gcattttttg catttgctct attccatgtt ctagatcctc tggtccgtag acttgttcac
    14041 tcgttgggga aacccagatt tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa
    14101 aataccgcat agaatctatc tctacatcaa atagttctac acgaacagga taggttgtcg
    14161 ttaacgggta aatatcgata atgcttcctc gaatactgaa ttctcccggt tttgcgacca
    14221 tctcttctcg tacatagccc atcaaagtca attggcgagg aagattttcc acgtctatct
    14281 catcaccaat atgccaattc aattgcgcct gtagccaagt tttcttattc ggcagtcgtt
    14341 tgtgcaaaga tgcgactggc aacaaataga tacctgcttt tttttcttga agtgcgttaa
    14401 gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa ggccatttca gcagaaagca
    14461 cttcatccac tggaaataaa tggatctgat cttgcggaat cacatgttgc agatcttctg
    14521 ctaactgatt cgcataatac aaatttggca tcagaataat catgttttta tttgttttct
    14581 taaacgctcg agcaaataga agtgttttcg ctgagcccgc taatcctgtg attagttgtc
    14641 taggcatttt ttcttctaaa ttgctgaccc attcttttac ttgcggatct tttccgacta
    14701 attgaataat atccattcct tttcctcctt cctcaattga atcgattcat cgcatccacg
    14761 aacgaatgcc cttctatgag atattcgact gctttaacag attgacacat gctttcttct
    14821 atcaaaggct gattttcttt gctgaaagga ctaagcacat gctggactac tgtttttttc
    14881 ccttctggcc tgccgattcc caccttgatt cgatcaaaga cattcgtatt caaatgggaa
    14941 ataatacttt tgataccgtt atgcccgccg gcacttccct tttgacggag ccggattttt
    15001 cccactgcca gatccaaatc atcgtagatt acgactaatt cctctggata aatcccaaaa
    15061 taagtcatta gtgggccgac tgcacgacca gattcattca tgaatgtttg tggtttgact
    15121 aaaaggatct tttctccatt atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt
    15181 ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga ccataaagcc gatattgtgt
    15241 tttgtatatt ggtatttggt acctgggttt cctaacccga cgatcatttt catttttgtt
    15301 ttccacctaa ttctaaaaag cagcacaaaa ggctggactg ctaattagcg gtccagtcca
    15361 tcgagctttt ttctttttta cacttgtcaa tgcatagtat aacacaaaac aaggcgtttc
    15421 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
    15481 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
    15541 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
    15601 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
    15661 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
    15721 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
    15781 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
    15841 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
    15901 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
    15961 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
    16021 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
    16081 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
    16141 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
    16201 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
    16261 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
    16321 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
    16381 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
    16441 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
    16501 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
    16561 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
    16621 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
    16681 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
    16741 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
    16801 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
    16861 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
    16921 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
    16981 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
    17041 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
    17101 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
    17161 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
    17221 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
    17281 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
    17341 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
    17401 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
    17461 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
    17521 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
    17581 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
    17641 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
    17701 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
    17761 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
    17821 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
    17881 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
    17941 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
    18001 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
    18061 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
    18121 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
    18181 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
    18241 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
    18301 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
    18361 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
    18421 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
    18481 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
    18541 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
    18601 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
    18661 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
    18721 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
    18781 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
    18841 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
    18901 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
    18961 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
    19021 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
    19081 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
    19141 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
    19201 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
    19261 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
    19321 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
    19381 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
    19441 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
    19501 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
    19561 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
    19621 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
    19681 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
    19741 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
    19801 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
    19861 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
    19921 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
    19981 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
    20041 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
    20101 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
    20161 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
    20221 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
    20281 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
    20341 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
    20401 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
    20461 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
    20521 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
    20581 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
    20641 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
    20701 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
    20761 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
    20821 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
    20881 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
    20941 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
    21001 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
    21061 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
    21121 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
    21181 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
    21241 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
    21301 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
    21361 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
    21421 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
    21481 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
    21541 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
    21601 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
    21661 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
    21721 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
    21781 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
    21841 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
    21901 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
    21961 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
    22021 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttg
//
LOCUS       DAZKMZ010000043        21835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000043 DAZKMZ010000000
VERSION     DAZKMZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.043"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG8M5_001689"
     CDS             complement(41..1432)
                     /locus_tag="ACG8M5_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0358944.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG8M5_001690"
     CDS             1689..5078
                     /locus_tag="ACG8M5_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0358945.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG8M5_001691"
     CDS             5081..6502
                     /locus_tag="ACG8M5_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0358946.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG8M5_001692"
     CDS             6499..8376
                     /locus_tag="ACG8M5_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0358947.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG8M5_001693"
     CDS             8468..9310
                     /locus_tag="ACG8M5_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0358948.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG8M5_001694"
     CDS             complement(9462..10337)
                     /locus_tag="ACG8M5_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0358949.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG8M5_001695"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG8M5_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG8M5_001696"
     CDS             11663..11968
                     /locus_tag="ACG8M5_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0358950.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG8M5_001697"
     CDS             12147..14162
                     /locus_tag="ACG8M5_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0358951.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG8M5_001698"
     CDS             14164..14448
                     /locus_tag="ACG8M5_001698"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0358952.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG8M5_001699"
     CDS             14474..15847
                     /locus_tag="ACG8M5_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0358953.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG8M5_001700"
     CDS             complement(15930..17945)
                     /locus_tag="ACG8M5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0358954.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG8M5_001701"
     CDS             complement(17967..19181)
                     /locus_tag="ACG8M5_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0358955.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG8M5_001702"
     CDS             19401..20345
                     /locus_tag="ACG8M5_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0358956.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG8M5_001703"
     CDS             20641..21585
                     /locus_tag="ACG8M5_001703"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0358957.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7421 a         3994 c         4119 g         6301 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacggcaaat acaatgattc atatc
//
LOCUS       DAZKMZ010000044        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000044 DAZKMZ010000000
VERSION     DAZKMZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.044"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG8M5_001704"
     CDS             291..1430
                     /locus_tag="ACG8M5_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0358958.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG8M5_001705"
     CDS             complement(1497..2072)
                     /locus_tag="ACG8M5_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0358959.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG8M5_001706"
     CDS             complement(2077..3051)
                     /locus_tag="ACG8M5_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0358960.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG8M5_001707"
     CDS             3334..4302
                     /locus_tag="ACG8M5_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0358961.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG8M5_001708"
     CDS             4415..4693
                     /locus_tag="ACG8M5_001708"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358962.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG8M5_001709"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG8M5_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0358963.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG8M5_001710"
     CDS             5150..5905
                     /locus_tag="ACG8M5_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0358964.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8M5_001711"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG8M5_001711"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0358965.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8M5_001712"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG8M5_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0358966.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8M5_001713"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG8M5_001713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0358967.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8M5_001714"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG8M5_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0358968.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8M5_001715"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG8M5_001715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0358969.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG8M5_001716"
     CDS             10642..11184
                     /locus_tag="ACG8M5_001716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0358970.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8M5_001717"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG8M5_001717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0358971.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG8M5_001718"
     CDS             12777..13679
                     /locus_tag="ACG8M5_001718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0358972.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8M5_001719"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG8M5_001719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0358973.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG8M5_001720"
     CDS             15122..15544
                     /locus_tag="ACG8M5_001720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0358974.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG8M5_001721"
     CDS             16013..16243
                     /locus_tag="ACG8M5_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0358975.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG8M5_001722"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG8M5_001722"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0358976.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG8M5_001723"
     CDS             17654..17830
                     /locus_tag="ACG8M5_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0358977.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8M5_001724"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG8M5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0358978.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG8M5_001725"
     CDS             18325..18708
                     /locus_tag="ACG8M5_001725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0358979.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG8M5_001726"
     CDS             18877..19761
                     /locus_tag="ACG8M5_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0358980.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG8M5_001727"
     CDS             19875..20111
                     /locus_tag="ACG8M5_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0358981.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG8M5_001728"
     tRNA            20199..20279
                     /locus_tag="ACG8M5_001728"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG8M5_001729"
     tRNA            20286..20357
                     /locus_tag="ACG8M5_001729"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKMZ010000045        18746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000045 DAZKMZ010000000
VERSION     DAZKMZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.045"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(325..1617)
                     /locus_tag="ACG8M5_001730"
     CDS             complement(325..1617)
                     /locus_tag="ACG8M5_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0358982.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1614..2237)
                     /locus_tag="ACG8M5_001731"
     CDS             complement(1614..2237)
                     /locus_tag="ACG8M5_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358983.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2240..3076)
                     /locus_tag="ACG8M5_001732"
     CDS             complement(2240..3076)
                     /locus_tag="ACG8M5_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358984.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3093..3980)
                     /locus_tag="ACG8M5_001733"
     CDS             complement(3093..3980)
                     /locus_tag="ACG8M5_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0358985.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3996..5279)
                     /locus_tag="ACG8M5_001734"
     CDS             complement(3996..5279)
                     /locus_tag="ACG8M5_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0358986.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5962..6645)
                     /locus_tag="ACG8M5_001735"
     CDS             complement(5962..6645)
                     /locus_tag="ACG8M5_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0358987.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6663..7280)
                     /locus_tag="ACG8M5_001736"
     CDS             complement(6663..7280)
                     /locus_tag="ACG8M5_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0358988.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7290..8114)
                     /locus_tag="ACG8M5_001737"
     CDS             complement(7290..8114)
                     /locus_tag="ACG8M5_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0358989.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8107..8667)
                     /locus_tag="ACG8M5_001738"
     CDS             complement(8107..8667)
                     /locus_tag="ACG8M5_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0358990.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8841..9797
                     /locus_tag="ACG8M5_001739"
     CDS             8841..9797
                     /locus_tag="ACG8M5_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0358991.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10102..11058)
                     /locus_tag="ACG8M5_001740"
     CDS             complement(10102..11058)
                     /locus_tag="ACG8M5_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0358992.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11162..11764
                     /locus_tag="ACG8M5_001741"
     CDS             11162..11764
                     /locus_tag="ACG8M5_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0358993.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11852..13351)
                     /locus_tag="ACG8M5_001742"
     CDS             complement(11852..13351)
                     /locus_tag="ACG8M5_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0358994.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13367..13891)
                     /locus_tag="ACG8M5_001743"
     CDS             complement(13367..13891)
                     /locus_tag="ACG8M5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0358995.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14189..14938
                     /locus_tag="ACG8M5_001744"
     CDS             14189..14938
                     /locus_tag="ACG8M5_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0358996.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14929..15714
                     /locus_tag="ACG8M5_001745"
     CDS             14929..15714
                     /locus_tag="ACG8M5_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0358997.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15683..16399
                     /locus_tag="ACG8M5_001746"
     CDS             15683..16399
                     /locus_tag="ACG8M5_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0358998.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16501..17520)
                     /locus_tag="ACG8M5_001747"
     CDS             complement(16501..17520)
                     /locus_tag="ACG8M5_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0358999.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17539..18540)
                     /locus_tag="ACG8M5_001748"
     CDS             complement(17539..18540)
                     /locus_tag="ACG8M5_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0359000.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         5623 a         3934 c         3280 g         5909 t
ORIGIN      
        1 acgtgaaggc agtttgccat atttgaataa aaaatgttgt ttcaaatatt gaaaacagtc
       61 taacactact ttctgaaact gacaacgtgg tttacttatt tttttgtaaa atctaatgat
      121 ttttaatgca ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct
      181 aaaaaagcag cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc
      241 gaataaatag tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg
      301 gaagtaattt ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac
      361 tgcttttcct gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc
      421 atctacgtga gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc
      481 atctaataaa cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc
      541 atatggctca ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc
      601 ctttttatct ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc
      661 tttgctgcca ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata
      721 cgtcaaaatc gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat
      781 atcatcggaa aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata
      841 catttctcgc aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag
      901 ttcgcttcca tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat
      961 cccataaaag tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa
     1021 attcactaga tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt
     1081 atttcgatgc catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa
     1141 gtggatagaa aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg
     1201 atacggtgca taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac
     1261 cgttattatt tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc
     1321 atatcctttt ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat
     1381 aatgacttca gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt
     1441 ctttactttt ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt
     1501 ttgagataca tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc
     1561 ctcataaaaa aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta
     1621 aagtcattct ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt
     1681 aatgcagtga gtaaaaaatc atgctgtata caccagaaca gtccatacaa aaggcataaa
     1741 taccacagaa accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa
     1801 acaagactat ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg
     1861 gatacaaaaa gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc
     1921 actaataaat accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg
     1981 aaacacatcc gtttgatttt tcctttcctc caacatacct ttttttctgc caagtaatgg
     2041 acagatagat aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca
     2101 gacagcacta atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg
     2161 ataagactgg ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct
     2221 tctattggct ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat
     2281 atttattgaa gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa
     2341 acatactcgc gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata
     2401 ccgtgtaaga tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc
     2461 aataaaactg gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt
     2521 tggtaaagac actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg
     2581 ggatgctgac catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg
     2641 gtaaaatcag cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg
     2701 gaatcaaaat cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc
     2761 tttttccttt gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg
     2821 tccctatcgt cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa
     2881 ttccagcaaa ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca
     2941 aattcagcga accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata
     3001 agaccggata aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg
     3061 ttactgtatt attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat
     3121 ttgaaaatca gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta
     3181 gcatacccca tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc
     3241 gtggcatcat tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc
     3301 gcgattgtct gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc
     3361 ttgtaaaacg tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct
     3421 gggatctctt tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac
     3481 atcaaaacaa gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg
     3541 cctaatcgtt ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact
     3601 agagcgatag aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa
     3661 gtcgttcctt tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc
     3721 acaggtacag atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca
     3781 tcactcagca tctttatata attatccaag ccgatccatg agactggatt tcccaaacga
     3841 taatcggtga acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat
     3901 cctaggatga atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt
     3961 tttagttttt cttttttcat gccttcctcc tgctgctatc ctagaatgtt tgacgcaaac
     4021 tcataaagac tctctgctgc ttcttctgct gttacttctt cataaatgac tttttcaact
     4081 aaaagattgt attggtcttg aatttccgga ttcaaagcac tatcaaagac attcacttcg
     4141 ccgttgtcca tatcggaaat catttctaaa taatcaaatg tcgcttgcaa agttgcatca
     4201 tctgcacgag cgagctgttc tcgaatattc gacatgattg gtacaccacg ttctcctttt
     4261 aaaatttcat tggcttcttc actgttttga aagaaatcaa taaacttagc agcttcttct
     4321 ttatgggctg agctttttgc catactcagc atttgcgaag aacgtaaagg gataccagat
     4381 tctccatctg cttttctgcg aggtggatta atcaactgaa tctccatatc ttcaggagcc
     4441 cctgacaata tttctgtcaa ctggttactt gatagaaaca ccattgccgc ttctctatcc
     4501 ctaaccgcct gaaaagacag atccgtcgta cttgcagctc caccacctgg ttctggatgg
     4561 gcccctttgt ctaccagatt tttacgcagc tggaaaaaat ctgttagata gtctggattt
     4621 tcaaatccca agctgttaga tagatcttcc cgattataaa aattcaatcc tttctcaact
     4681 tgtggtattt gaataataca gccagctata aaatcttcga atcttgaaat agcatagatt
     4741 cctaattctt cagtgatttg atagctgatt tgttcaaatt catcccatgt ccaatttgaa
     4801 gctggttcag ggatatttaa ttgttcaaat atatttttat tgtaagcaat cgcaaacgaa
     4861 ttcaccccgt tagacagtcc aactaattgc ccgtcgtaag tcgtcgttgc taaataggat
     4921 tcatctgcat tagaaatatc aattatccct tgctccacat attcatctaa tggttcaatc
     4981 tgattgatgt attgaggaaa ttgattgttc agttggaaaa tgtcccagat atcatctgat
     5041 gctgctagtg tacctaattt tgtgtaatat ccttcataat cataaaattc ataatcgatc
     5101 gtcacatggg gatttttttc ttcataaagc tcgattactt ttactgttgc atcgtgtcgg
     5161 acttgcgaac cccaccaagc catgttcagc gtgatttttt tgtcatcttc ttgcccacaa
     5221 gccgtcaaga agaataacat aacacttaaa gctaagatga ttctcattct tttcttcatt
     5281 ctctgcacct ctacttccat tgttattttt caatatactg tatttttata cagaacatat
     5341 ttattaaacg atctttttca aggggaataa aaaccaagac ttgccttatc gttctgccta
     5401 ggatgaacaa ctcaatactt tcaagaaaaa ttagatagtg ctgattcttc tttcatcgct
     5461 aaaaacactt cttgcttcaa tgatgggagg ataattacgc aaagaaaaaa ggatatcaga
     5521 caactgccca atatccttga catagctaaa taagcatgct tcttcgtgtg cgctcttccc
     5581 taaaaaacag aaaaataact agtaattctg tatgaaatta ggtacaatat acacgtcgat
     5641 gcacaagcat tgactatggc aacttgattg gtcagttccc gctgactttt caagccccga
     5701 caatttagct gctactaaat tgtcgggtgc cttttttagt ttttattcgt ttttcattct
     5761 atagaaagcg cttcaataaa tcaagacatt tttttgttag aatgctaata ataacaaaag
     5821 gttttaactc ttcttatgta cattacaggt aaaaaaacaa aaaaatttct cttttttttg
     5881 aattctgaac aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc
     5941 tcgtcttttg attctcacct gttattcatg attctttttc tcagtttccc gatatttttc
     6001 cattttacga ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc
     6061 tgcttgaatg gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag
     6121 ttcaggatat tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat
     6181 attgataatc gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac
     6241 cgtactgata actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt
     6301 aggaccgttc aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga
     6361 agaacgagca ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc
     6421 ctcaataatg cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc
     6481 attcgcattt gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa
     6541 ttttccaaat atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc
     6601 ttttctcaat tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt
     6661 tgctatctcc ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc
     6721 gcataaccgg ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag
     6781 ttggtggccg taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac
     6841 agttgtgtat ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg
     6901 atagttatcc attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc
     6961 ccataagcct tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa
     7021 tttgattcta atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt
     7081 agttcttggg catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt
     7141 tcttgctgat tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag
     7201 agtatcactg ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt
     7261 ttccactttt ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct
     7321 tctagcgtca attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag
     7381 tgccaaggtt catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata
     7441 aatccatact gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt
     7501 gcatccaata cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc
     7561 agacctgtgt ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc
     7621 gcctcctctt ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact
     7681 tgttgttggg atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct
     7741 tcagcagcct tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg
     7801 ttacttaatg atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga
     7861 ccaaccaaaa ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga
     7921 tcttttgttt ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta
     7981 gcagttgttt tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca
     8041 tcatcgctaa aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct
     8101 aaaaatttat tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg
     8161 aacgtaagtt tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc
     8221 gatctgtttt tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc
     8281 atcatattga atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa
     8341 agggatcagc tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt
     8401 gatttgcttg tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg
     8461 caaaatcttt tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat
     8521 cgcttcagat ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat
     8581 cagcaagtat tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat
     8641 aataagatca atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag
     8701 ttttgatcaa aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct
     8761 ctcctcttta ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa
     8821 ctatgaagga gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca
     8881 tcgcacatga gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt
     8941 ccagaacttc agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg
     9001 gaagctatca agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa
     9061 atagacagca tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg
     9121 aaaaagccat cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa
     9181 agaatttgat cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac
     9241 gctcaataat ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca
     9301 gttacaaaga accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc
     9361 tgttggatat cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg
     9421 aagtagttgc ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta
     9481 tcttgcgtaa caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc
     9541 caaaagtcgg tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg
     9601 ctgatcgtgc ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata
     9661 catctgaagc aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc
     9721 ccaatcgctc tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc
     9781 tatggaaaaa tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag
     9841 aacagccaaa caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag
     9901 gaaagctttt gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca
     9961 caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga
    10021 ggagttgaca ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa
    10081 tattagctga attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat
    10141 ttcaataaaa cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc
    10201 agggcgacta gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt
    10261 taaaatttgt ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc
    10321 atagatatgt tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc
    10381 tttaatcaca ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg
    10441 tctgagccag tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt
    10501 cagtaaatgt tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc
    10561 catttgtctc aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg
    10621 cttcgtacga tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat
    10681 taatcccgct tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt
    10741 gaccaaaacc gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat
    10801 gaacaggaat aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa
    10861 cagtgctgtt gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt
    10921 agagcgcttg gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa
    10981 cagcgataag ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt
    11041 tcgcattcct atacgcattt acttccccct tttttatttt aattatactt gaataatgaa
    11101 aaactagcta tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc
    11161 tatgcaattt ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt
    11221 tttatgtatc ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc
    11281 tgctgttttt tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat
    11341 catcggcaat acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt
    11401 tcacaatgat tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt
    11461 cgtgtcagat ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct
    11521 gattgcgttg agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga
    11581 tacttttata gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac
    11641 agaaaagaag gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct
    11701 aagaacaaat ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga
    11761 ctaattttct tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc
    11821 catttttgat tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg
    11881 cctcttttgg cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct
    11941 ctacgatttc cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa
    12001 gcaaatttgt ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc
    12061 ctgtatcgac aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca
    12121 attgcggtag catatcgata accatactca agacagctgc ttcgccatat tccttgaagg
    12181 cattggcgat cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg
    12241 cttctgcttg tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac
    12301 ggaatcgttc tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt
    12361 ctttagcgta ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac
    12421 gacgtgtgat ttctttttct tccagttcga tttgtttttg acgttctacg attttaatct
    12481 gcatttcctg ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt
    12541 ctgcttttgc tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt
    12601 cttttaatga ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt
    12661 ctgcttgagc ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat
    12721 ctcttttgac ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc
    12781 tgacctcttt gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag
    12841 cgacttcttg tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca
    12901 tagaaccaag aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt
    12961 cttctcttgt cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac
    13021 cgattttgat gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt
    13081 caggggtgga gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa
    13141 ctggcaagac gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa
    13201 tgtgcacatt tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg
    13261 ctgtctggta cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg
    13321 cgatcgcgat aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca
    13381 tgctttcata aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg
    13441 ttccccgctc agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac
    13501 cggtaacact tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa
    13561 cagctacttg tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca
    13621 tcggcatcaa tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag
    13681 caataaataa aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc
    13741 ttgtgaaagt taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata
    13801 catctccaaa aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat
    13861 aaatcgtctc aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta
    13921 tcataccaat gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat
    13981 aggaaggttg tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc
    14041 acaataaatt ctttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct
    14101 ccagaacacg atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc
    14161 catattgatc aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc
    14221 tagcattgct aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga
    14281 cccgacacat gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt
    14341 tatcgttgtc atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg
    14401 tcttcctttc tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc
    14461 tgaaaaagaa ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca
    14521 agagattatt ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa
    14581 agatggacaa aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg
    14641 cgccgttttt cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat
    14701 agcagaacag ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc
    14761 catctcttct gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca
    14821 aacagagcag ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt
    14881 ttgtttaaag agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat
    14941 gggatagaaa ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca
    15001 gtcgcaactt tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg
    15061 aaacaatata taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc
    15121 atttctggtt tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta
    15181 aatcaaaatt ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt
    15241 gccggttttt tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta
    15301 gaaagcgatt cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa
    15361 gtgttaggga acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac
    15421 ggacaggtcc ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc
    15481 tgcgatgacg gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt
    15541 tcgacgaagg gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc
    15601 cacggtggat ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa
    15661 ttaccaaaaa aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat
    15721 ggttgcagaa ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag
    15781 tttcagaaat gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct
    15841 gcttgatgta tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca
    15901 aggaaatgat atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa
    15961 tgtgatccgc tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca
    16021 gctggcgatc gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagccga
    16081 tcagaaattg atcgatcaag tattcaacca caccactccc attcacgaaa taccatcatt
    16141 catcgatttg cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg catttattct
    16201 aaaagaagat actatttttt caacaaaaga actggctcaa aaaagctcac tgtcacgtat
    16261 atccgtcaag aaatatttga attttttagt agaacaagat atattgaacg aaagaatcaa
    16321 atatttggaa atcggccggc cgctaacctg ttatgaattg acaaagaatg ggcggaaaaa
    16381 gctcacagat tactgttgag tcatcgaatt atcccataca aaatagtcca agtatacaga
    16441 agtaacaact gtttacttgg actattttta aacattttat tgacgaatat ccatgttttc
    16501 ttatcttaat tgacgggctt taggaagaga tacctcattt ttcgtttttc cagtttctat
    16561 tatttcacga aagttctcga aacttgtcga aatcatattt gataatgcct catctgtatt
    16621 tgaacctaca tgcggggtta caagaactct agggtacatt tctactaatt gctggatagc
    16681 agggtctgga atcgtctccc atggcttgaa ggatcggaaa aaaacctctg tttcatttgc
    16741 aaaaacatcg gttgcaaaac cagctaaatg attgcttttc aaagctctta aaatagcttc
    16801 attatcttga agctctccac gggctgtatt aatcaagatg ctaccttttt tcatttgagc
    16861 taaaaatcct tcattgatca tttgatcgtt ttcacctggg atatgaggta catgaatact
    16921 gacaatatcg cttttggcta ataactcttc aagcgagtgg aacgtcaata cttttttagc
    16981 agcttctgtc tgatgaatat cgtagccgat cactttggct cctagtccct taaacaaaac
    17041 agcttccgtc aaccctatcc tgcctgttcc tatgatccct actgtgcaat ttctgatttc
    17101 acgactaaac atttgctcat ccactcgaaa atctttgaaa gatgttcgga tcgtcgtata
    17161 cgcagtattt ctcaaaagca tcatggcaaa agtcaaagat aactctgcta tcgcatttgg
    17221 cgaatacgat ggtacacggg ctactgtcat accaaaatca gctgccgctt gtagatcgat
    17281 atgattgact cccacagtcc gtgtaaaaac ataacgaatc ccatattcct gcatttttgc
    17341 aagattggtc tcatctgcta tacaatttcc cctcaacaat acagcatcat ggtcctttgc
    17401 tgacacgata ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat aatggtaaat
    17461 attcaatttt tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg cgattctcat
    17521 actttcttcc tccatttctt atgaaaataa acctatgcca tgaatcattt gtaatacaac
    17581 gatccataca ggtgtcagta ccacagcaaa aatagttgac agtaaggatg cattggaggc
    17641 taaaagtgcc tccctatcaa aactgatagc ataggctgct gccactgttg cagctggtgt
    17701 agccatcatg ataacgattg ttgccagtgc ttcaaaattg actggtagaa tattcgttgc
    17761 cgtcaaaact gctagaatca cgatattcaa tgcaggtaca aataccactt tgactagact
    17821 gtaataccat gaagtttttt cagatgcagc ttttttgaag cttacttcac ctaatgttgc
    17881 gccgattgct agccacgcca atggtgaact taaaccagct aaataatcca tcgactgaaa
    17941 tagccacggt gcagtctggt caattcgcaa aaacgctact tggcttatct ctccttctgc
    18001 acctgtaact gacacttgtg gcagataccc ttggaacagc caaatgaaaa ggccggcaaa
    18061 agtagcgata atgattggat tgacgaacat cgctttgata tttttagctt ccatcttcaa
    18121 gccactcatt tttatgtatc cataagaata caaaaagatt cggtagccaa tattgaaaat
    18181 tgaggaatac atcacgccga caggaccata aatcgcactg acgataggcg ttccgaaaaa
    18241 ggtcgtagaa ccaaagatcg tcaggacacg caacgttgtt tgcttatccc ctttatagct
    18301 taagaaaaaa ggttttgaaa ctagaataag aataatataa ataactaatc cccaaatcaa
    18361 gacattgatt ccttgcatca gtaattctgt attgatatct tgcatgaaag ctcgaaatgc
    18421 cagtgctggc aaagccactg ttaagacgac tttcgataga atttttccaa cagattcaga
    18481 aaaaatcccc ttttttctac aaacaaaccc taataatata ataaaaactg ttgaagtgat
    18541 tgcactgaca atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata agttcatctt
    18601 tctcctccta attatgttca tttattcaca ataaaaaaag cttttatttt gctaatacgt
    18661 attaacattt ttaattttat ctttacattg ttacttattg aacaatagga aattaaagaa
    18721 acttttgaaa cttatgtaaa gtgatt
//
LOCUS       DAZKMZ010000046        18693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000046 DAZKMZ010000000
VERSION     DAZKMZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.046"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1753
                     /locus_tag="ACG8M5_001749"
     CDS             68..1753
                     /locus_tag="ACG8M5_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0359001.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1767..3050
                     /locus_tag="ACG8M5_001750"
     CDS             1767..3050
                     /locus_tag="ACG8M5_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0359002.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3120..3989
                     /locus_tag="ACG8M5_001751"
     CDS             3120..3989
                     /locus_tag="ACG8M5_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0359003.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3986..4822
                     /locus_tag="ACG8M5_001752"
     CDS             3986..4822
                     /locus_tag="ACG8M5_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0359004.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4848..5915
                     /locus_tag="ACG8M5_001753"
     CDS             4848..5915
                     /locus_tag="ACG8M5_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0359005.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5912..6739
                     /locus_tag="ACG8M5_001754"
     CDS             5912..6739
                     /locus_tag="ACG8M5_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0359006.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6784..7809
                     /locus_tag="ACG8M5_001755"
     CDS             6784..7809
                     /locus_tag="ACG8M5_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0359007.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7806..9989
                     /locus_tag="ACG8M5_001756"
     CDS             7806..9989
                     /locus_tag="ACG8M5_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0359008.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            9989..11119
                     /locus_tag="ACG8M5_001757"
     CDS             9989..11119
                     /locus_tag="ACG8M5_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0359009.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11116..11793
                     /gene="pgmB"
                     /locus_tag="ACG8M5_001758"
     CDS             11116..11793
                     /gene="pgmB"
                     /locus_tag="ACG8M5_001758"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0359010.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11877..12404
                     /gene="msrA"
                     /locus_tag="ACG8M5_001759"
     CDS             11877..12404
                     /gene="msrA"
                     /locus_tag="ACG8M5_001759"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0359011.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12605..13291)
                     /locus_tag="ACG8M5_001760"
     CDS             complement(12605..13291)
                     /locus_tag="ACG8M5_001760"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0359012.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13371..14909
                     /locus_tag="ACG8M5_001761"
     CDS             13371..14909
                     /locus_tag="ACG8M5_001761"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0359013.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15029..15496
                     /locus_tag="ACG8M5_001762"
     CDS             15029..15496
                     /locus_tag="ACG8M5_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0359014.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16030..16458
                     /gene="rplM"
                     /locus_tag="ACG8M5_001763"
     CDS             16030..16458
                     /gene="rplM"
                     /locus_tag="ACG8M5_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0359015.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16474..16866
                     /gene="rpsI"
                     /locus_tag="ACG8M5_001764"
     CDS             16474..16866
                     /gene="rpsI"
                     /locus_tag="ACG8M5_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0359016.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16954..18180)
                     /locus_tag="ACG8M5_001765"
     CDS             complement(16954..18180)
                     /locus_tag="ACG8M5_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359017.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(18252..18542)
                     /locus_tag="ACG8M5_001766"
     CDS             complement(18252..18542)
                     /locus_tag="ACG8M5_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359018.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
BASE COUNT         6046 a         3410 c         3945 g         5292 t
ORIGIN      
        1 acagtaagcg ttttccataa tatgatcaaa tcgttgggaa cgctcccaaa aaagaaaggg
       61 gaatccgatg tatacaaaac ttttacaagc gatttatcgc gactcagatt tgtctgagaa
      121 aaccgcctct atgattgaac aaaaagtcag tgtttatcag cagaagaaag gccaggcagg
      181 caaactgcat tgggacgaag aagatatttt cttgattact tatggggatc aattttttga
      241 agagcagcaa ccaactttgc gtacatttaa gaaattttat caaaagtatt tggcagatac
      301 atttagagtt gtacatttct tgccattttt cccatataca tctgatgatg gtttctccgt
      361 tgtaaattat caggcaatca acgaccgttt gggcacatgg gaagatatag aagaaatgag
      421 aagcgaaaca cggttaatgt tcgactttgt ctgcaatcat atgtctgccg aaagtacttg
      481 gtttcaggaa tatttaaact tgaatgatac gtacaaagat ttttttattg atttgccact
      541 ggatacagat acaagcagcg tgactagacc aaggaccagc ccgttactta caacgttcat
      601 tgacaaaaac gggaaagaaa aacacgtatg gacgactttt agcgaagatc aagtagactt
      661 gaattttaaa aatccagaag ttttattgaa aatgatcgat attcttttgt tttacttaga
      721 aaaaggcgct tcatttattc gcttggacgc tgtgggattt ttatggaaag aactaggaac
      781 ttcttgtata cacctgccag aaacgcataa aatcatccaa ttattccgtg caatcgctga
      841 tgaagcagct ccaggaacgg tattgatcac tgagacaaat gtgccacaca aagataatat
      901 cagttatttt gggaatggcg agaatgaagc acagatggtc tatcagtttt cgctaccacc
      961 tttgaccctt caagcgattc gttcgggtaa tacaagtaag ttgaaagaat ggttaaaaac
     1021 catcgatttc gcatcgaaaa aaacaacttt ttttaatttt ttagcctccc atgacggtat
     1081 cggattgaat ccggtacgag gtattctgcc ggaaaaaacg atccttgaat tagtggaaca
     1141 gctgaagaaa gagggtaccc gagtaaacta caagaaaaac ccagatggaa cgaccagtcc
     1201 atatgagata aacgctacgt atctagatgc gttaagttct ccaacagata cgacggaatg
     1261 gagaagcaaa cgctttttag ctgctcattc atttttggta acgttaccag gcgtgccggc
     1321 aatttatatt caaagtgtgc tgggaagtcg aaatgattac aaaggcgtag aaaaaacagg
     1381 agagaatcga aaaatcaatc ggcaaaaatt tttgttagca gatattgaga aagagttgca
     1441 gacagatcca ttaagaacag cagtatatgg tgaattgacc cagatgctat ctattcgaaa
     1501 aaaagaaaaa gccttccatc cgaatagttt gatttactta ctggactcag cagattcttt
     1561 tatcagtttt attcgcgaag cgaaggaaga gaaagtctta gttatccata atttttccaa
     1621 tcaatcagca ggttatcttt taccaaaagg agattaccta gacctgtttt ctggacaaat
     1681 tatccattgc agtcaagaac gaaagttgat attccagccg tatgaatata tgtggctgaa
     1741 aataataaaa taagaggtgg aaaaaaatga aaaaaattat tgttactacc tgtctgctag
     1801 ctactggatt tcttcttact gcttgtggtg aaagtcaaag ctctaacgaa tcacaacaga
     1861 caacgattca attcatgcac tcttctgtcg aacaggaacg attaagtgtg atcaatgaac
     1921 tggttgctga ttttgaaaaa gaaaatccag acatcaaaat caagcaagtg ccggtagaag
     1981 aatcagcgtt caatacaaaa atcgttacgt tagctcgttc cggtaaatta ccagaagtaa
     2041 tggaagtcag tcaggatttt gctaaagtga tggataaaga tgaactgatt gatcagacgg
     2101 cagtccaaaa cgttatcgag gaagctggag aagataatta ttatgacgga gccaaaaacc
     2161 ttgttcgttc agaagatgga aaaagctata ttgctgcacc aattagcggc tgggttcaag
     2221 gtatttggta tgacaaagaa aaattatcag aagctggttt ttcagaacca gaaaattggg
     2281 atgatatttt aaaaattgct caacatttca cagataaaga gaataaacaa tatggaattg
     2341 ctatgcctac tgctgaagga acaatgagtg agcaagcgtt ctctcaattt gctttgtcaa
     2401 atggagcaaa tgttttggat gatgaaggcg aggtaacgat cgatacggaa aagatgagag
     2461 aagcattgtc gttttatcaa aacttatccc aatacacgat gccaggctct aatgatgtga
     2521 cagaaatcaa agatgctttc atgaatggaa cagcaccaat ggcaatctat tcaacttaca
     2581 ttttgccttc tgtttatgaa gcggggaact cagaaaatat cggttttgct atcccaacac
     2641 aaaaagatca agctgtctat ggcacagtat ctggattgac gatttcttct ggtctagacg
     2701 agaaacaaaa agaggctgct gaagcattca cagctttctt atctcaaccg aaaaacatgg
     2761 aaaagtgggt cttgatgtct cctggtggtg ctcaaccagt gaataaacaa gtagtcgaac
     2821 tggatggtta caaagaaaat gaagtcatca agtcttttgg cgagctgcca tctgagattg
     2881 ctagtgcttt tgatgaagta caagtctttg gactagtcgg ggaaaagaat ttcacgaaaa
     2941 tgggagatat cacaagctca ggtgtgatcg gaaaagcagt gaaccaagta actgtcggaa
     3001 acgaagatgt cgatcaagct ttagctgacg cacaaaaaaa tacgaaataa caagaagttc
     3061 tgccaatggt gttcgaaaaa agtagatagc tattggcaga ttatcagggg gaggtttcaa
     3121 tgaatcgtcc aaggaaacat tctgacttaa aattagccat gggactttta gcaccaagca
     3181 tgcttttatt agctgtttta gtgctgattc caatgctttc caatattcag atcagttttt
     3241 tcgatatgcc gctgaatcct aactcagacg ccgtttttat cggattaagc aattatcttg
     3301 aaattttaag tgatagtgaa ttttatcgtt ctttaggttt aacagttgct tacacattct
     3361 tagttgtagc aggcagtaca gctttaggat tagccgtagc tattttgctt aaccgcaagt
     3421 tcagatttag gaaaacggtg cgttcactga ttttattaag ttatgttacg ccatctattt
     3481 ctttgatttt tgcttggaaa tatatgttca ataatagtta tggaatcgtc aatgatctgt
     3541 tagtagatag gttgcagctt ttcaacgaag caccgctgtg gttcgaccag ccagttgcta
     3601 gcttctttat ggtcgttttg tttgctatct ggagatacta tccgtatgcg tttatttcat
     3661 ttttggctat tttgcaaaca gtcgacaaaa cgctttatga agcagctgaa atggacggtg
     3721 caaatgcttg gcagaagttc aagatcgtga cactgccggc aatcaaacca gtactagcaa
     3781 ctgtgattgt tttacgatct atctggatgt tctatatgtt tacagatgtc tatctactga
     3841 cgaataaagt caatatctta ggcgtttatt tgtatcagac agcctttgcg ttcaatgatc
     3901 ttggaaaagc agcagctata tcgattctat tgttcatcat tattgcagcg gtcgtcttgc
     3961 tctcacgaaa gaaggtggac ttgaaatgaa aaataaaaag agcaggaaaa tcttatttta
     4021 cgcgacgatt ttgttgctat tagctgtcaa tttgtttcca tttttgatca tgctgatgac
     4081 atcattcaag gattcacaag aagcaattgc aacgacacaa actatttttc cgaaagaatg
     4141 gacttttgaa cattatcagg atattttcaa tgctgagatt tttccattca tcatgtattt
     4201 caaaaatagt ttagtgatct ccttgatagc ctccgtaact gcagtagtga ttgggatcag
     4261 cggggcatac gctttgtcaa aactacgctt caaagctcgc tcgaaaatca atgctagctt
     4321 ttacaccgtt tatatgtttt ccggtatttt gatcgttgtt ccattattta aaatcatttc
     4381 cggtctaggt ttgtatgata ccaatacagc actgattatc acgatggtcg ttcaaacact
     4441 tcctactgcg atattcatgc taaaaagtta ttttgacacg attccgactg atattgaaga
     4501 agcagcaatg atcgatggtc ttagccggat ccagatcatt attcggatca ttataccatt
     4561 ggctatttct ggaatcattt ctgtcttcgt ttattgcttt atggtttcgt ggaatgatta
     4621 tttatttgct tcgattttcc tgtcgacctc cgaaaaattt actttaccaa tcggtttgaa
     4681 cacattattc agtacaccag actacatttg gggacgaatg atggccgctt cattggtgac
     4741 cgctttacca gtcatcatta tgtatgcgtt atctgaacga tttattaaag gtgatttagt
     4801 cgaaggcggc gtaaaaggat aaaaagaaaa gaaagaggaa agaaaagatg aaaaaattag
     4861 ttgcattaga accaagaata gccggattca aagattacga agaccgtgag atcgaaaaga
     4921 acgaagtacg tatccgagta attttcgcta ctccaaaacc aggaacagaa attgtcgact
     4981 ttagaggatt aagtccattt atcgatgaag aattttcaga agaatggcgc ctattcttgc
     5041 caagagaaga aggaagccaa cgcggtgtgg tatttggcga atttcaatta ggaaatatga
     5101 ttgtcggtac gatcgaagaa acgggcagtt tggtgacaga gtatcaaaaa ggagagttag
     5161 tctgcactta tggacccatt cgtgaaacgc tgattacaaa cggtgttgat aattataaac
     5221 tacgtaaact tgatcggttg gatcaatgga aaaatgcagt atgttacgat cctgctcaat
     5281 tcgctttagg tggtatccgg gaagcacatg ttcgagcagg tgatgctgta gcaatcttcg
     5341 gtttaggcgc tatcgggcaa attgcgattc aattagctaa aaaagcaggt gctactacca
     5401 tcgttgggat cgatcccata gcgcagcgta gagaattagc agaaaaatac ggtgccgatt
     5461 acacgctaga tcctatgaaa gcagatgtcg gttttcgtct aaaggaattg acagataaac
     5521 gtggagtaga ttcagttatt gaaacgagtg gcagtgctgc cgcattacaa gcttctttaa
     5581 gagggatcgg ctatggcggg atcatttctt atgttgcctt tgctaaacct tttccagctg
     5641 ggtttaatct tggtcgcgaa gcacatttca ataatgcaaa aatcgtcttt gctcgagcat
     5701 ctagtgaacc aaacccagat tacccaagat ggaaccgtaa acgaatcgag gacacatgtt
     5761 gggaattatt gatgaacggt tacttggatt gtgaagaaat cattgatcca gttgtgccat
     5821 ttgcagaaag cgcagaagct tacatgcgtt atgtagacca gcacccagaa gaaagtatca
     5881 aaatgggcgt tcaagtccga ggaggagaac aatgaaattc ggtacgcaag atcaagcatt
     5941 tttcccaaga gacattttag aaaaatttag atttatcaaa gactgtggat ttgaagaata
     6001 cgaaatcgac ggtaagttgt taatagaaaa tatcgacaca gtcaaacagg caatcaaaga
     6061 aacagggatc ccagtagtga cagcttgtaa cggctacgat gggtggatcg gtgactttat
     6121 cgaagaacgg cgtcttaatg gtgtacgtca aattgctgag attttgcgtg cactaagcga
     6181 ggttggaggc aaaggaatcg ttatcccagc agcttgggga atgttcactt atcgattacc
     6241 gccgatggtt tcaccccgaa gcaaagaagg agactataaa gcagtcagcg aatcacttcg
     6301 ttatttagat aaagtagccg aagaaacagg tacggttatt tatttggagc ctttgaaccg
     6361 ataccaagac catatgatca atttgttagc ggatgcgcgt cattacattg actctaatca
     6421 attgaaacat gtgcagatca tcgctgattt ttatcatatg aatattgaag aagatgatat
     6481 ttcaaaagca ttgactacgc aaaaagagta tgtgggtcat atccatttag cagataatca
     6541 tcgctaccaa ccaggatctg gcgcaattga ttttactcgc cattttcaaa cgctacttca
     6601 aaatgagtat gaaggttata tggtatatga atgtcgtttg cgaggagaaa atgaagtgaa
     6661 aatttatcag gaatcattgc aatacttgaa aaactgtctg gaaacagcgc gtcatttaaa
     6721 tgatttagag aaagaataga gaaagaataa caataagtaa ataagcaaag gagggagaaa
     6781 taaatgaaga aagtagcaat cataggcgct ggacaagtag cagaaaaagt acatgctgct
     6841 tattatcaaa aaagatcaga tgtcttagaa ttggccgctg tggttgatcc agtcagccag
     6901 cgagcagaag acttttgccg acgtacagga ttttctcatg cgtatacatc agttacacaa
     6961 atgctgaatg agatttctcc cgatatcgtg agtgtctgta cacccaatcg ctttcacttc
     7021 gaacatgtat tggctgccct cgaagctaat gcttcagtga tgtgcgagaa accaccagct
     7081 atgacagctg atcaagcaag aaagatggca gagacagccg ataaaaacgg gcagattttg
     7141 gcttatggat tccagcatcg tttttctgac gaagcaacaa tgatcaagaa tcatcttgat
     7201 gttctcgggg atatttatta cgtagaagcg aatgcgcttc gtcggtctgg tgttccaggc
     7261 tggggaacgt ttatcgacaa agaacttcaa ggaggcgggc ctttgatcga ttatggcatt
     7321 cacatgcttg atacagcgat gtatattact ggatttccta aagtaaaaag cgttcgggcg
     7381 tacagcttcc aaaaaattgg tacgaaaaaa aattcaggga aattcgggaa atgggatcca
     7441 agcaaattca cagtagaaga tgcaattttt ggcacactgg aattagaaaa cggcaagatc
     7501 atccaactga atacttcgtt tgctttgaat atcagagaag acaaactgaa tgtggctttt
     7561 tgtggcgatc aggcaggtgc taatctgtat ccaggcgaac tattcgaaga tgtagatgga
     7621 aagctggttt ccttacaaaa gaccacgcca gttaatgacc cacatgaact aagtatggct
     7681 cattttgtga atcgggcgct cggtgaccag actcagatga ttgcagatgg ctggcaagga
     7741 tatgtcatcc agaatctggt ggaaaattta tatcgttcgg cagaaacagg agaagttatt
     7801 accttatgaa agtagaagaa aaacagtttg atttagagta tatcaataag tatgcgagtc
     7861 agatgaccat tggaaatgga ttttttggta tcagaggaag ccaggaggaa gactatcgga
     7921 atcaagtaag gggaatgttt gcagctggtg tatataaccg acctttagga gcagaatcag
     7981 ccgagttagt gaatctccca gatgtcatca gatgggaaat aaaaatcgac ggtgaacatt
     8041 tttctcttga gacgatgaaa gttcttgcat acgagcgggc actagattta gaaatgggag
     8101 agcttaaacg ctatgcgctc attgaaactg ctcaaggaaa aaaagtggaa gtcattactt
     8161 gtcgtgttgc tgatcaaaat aatctccatc aggcagccct gaaagtggtc atcaagccgt
     8221 tgaccgaaga ctgccaagta gatgtgaaaa cgggtatcga tggacagcaa accaattttg
     8281 gtacacagca gttggttgaa aaagaactgc gagtatttgg tgacgatttg atcagtgcat
     8341 cctaccagac aacagaaagt aaaattgata ttgggattgc agctaaattc aataaagcag
     8401 gtgtattcca agcaaaaaat cgccggatca ccgcttcctt ttcagaaaag atcagaaaaa
     8461 atacgtcctt tacgctggag aagatcatcg tgctctctac ctctcttcag caaacagacc
     8521 cgtttaagaa aagtattgcc caagcccaga aaagcgttag ttatacagaa attttagagc
     8581 agtcgatgtc ttattggaca gatttttggc atcagcatcg gataacgatc caaggcgatt
     8641 cttctttcga tcagttagcg atggacttcg ctgcttacca tttacagatt atgactcccc
     8701 gcaatgatcc aagtgttagt gtaggggcga agggactaac tggcgaaggt tataaagggc
     8761 atgttttctg ggatacggaa atttttatcc tgcctttctt tttatatcag tttcctgaag
     8821 tagcaaagaa tttgctgctg taccgttatg atcgattaga aggcgcacgg caaaaagcaa
     8881 gagattatca ctatcaaggt gcacttttcc catgggaaag cgcacacact ggtcaggaag
     8941 agacacccaa atttgctgca ttgaatatca aaaccggaac aagacaagaa gttgcttcag
     9001 ctgccgcaga acatcatatc agtgcagacc ttgcttatgc cgttcaagag tatgtccaag
     9061 caacagagga tcaatcgttt ttagaaacaa agggaaaaac gctcattcat gaaacagcag
     9121 ctttttggct gagcagagct gtaaaagtcg atgatcattt agagatacac agagtcattg
     9181 gtccagatga atacacagaa tatatcgaca ataatgcata tacgaactat ctggcgcatt
     9241 ataatgtggc cttagctgtt aaatatcaag ctggcgatcc gctttttttg aaagaatgtc
     9301 aaaatttttt agataagtta tatttgccag tgcctaacaa agaacagctg attccgcaag
     9361 atgacacgta tctcagtaag ccagttattg atttgaccac ttacaaagaa cgtcctggca
     9421 gccagtctat tttacttgat tatgcccgta gcgaagtaaa tgaactccaa gtcagcaagc
     9481 aagcagatct tgtcatgctg ttatacttat tacctgagta tttttctcaa ccaatcgttt
     9541 ctaagaattt gcattactat gaaaacaaaa caatccacga ctcatcgttg agtaaagcga
     9601 ttcacgccat agaggcttgc cgtatccaag aaatggaatg ggcgtatcat ttattccaag
     9661 aagcgtgcct gattgattta ggaccagatc cgcattcatc agatgacgga attcatgctg
     9721 cttcattagg agcactatgg ctagcagtta tatttggttt ttcaggtatt acagtaacga
     9781 atggaactct ttctatttct ccaagacttc cttcagcatg ggaaaaaatt tcttttcctt
     9841 ttatttggaa aaatcacagg ttgctatttg ttttgacaaa aaaacaaata gtaataaaaa
     9901 aagagacgaa agaaacatta cctatttttg tcaatggaga ccaataccag ctggaaaaag
     9961 aactgatctt ggaacggagg ggaaaataat gcgtattctg attgcgccag attctttcaa
    10021 agaaagtctg acagccttag aggcagcaca agccattcaa tcaggttttc aagaagtttt
    10081 tgttcaggct gaatatgatc tgctacctat aggcgatgga ggagaaggaa cggtttcatc
    10141 attagcagac agtttaaggt tgaagcggca aacagcagtc gttactaatg catttggcaa
    10201 aaacaaagaa atccagtacg cttcagacgg tcaaactgcg atttttgaaa tggcagaaat
    10261 cgtcggattg aaagatgtcc cactaaacca acaacagcca cttaggttat ccacgcgagg
    10321 agtaggagaa ctgatacgac aattgttgca acaaggtgtt cgacacatca tcatcggcgt
    10381 tggcggaagt agtaccaacg atggcggcat cggcatggca gaaggtctag gttatgcttt
    10441 ttacgatcaa gataatcaaa aagtagaagc actgggagaa aatctaggta aaattgctac
    10501 agttgataaa acgaacgtgc ctattgagct gattaaagcc aaaatcactg tcattactga
    10561 tgttactaat cttttatgcg gaaaagaagg cgcttctttt gttttcggac cacaaaaagg
    10621 cttgaaagaa aatgaattag cagtggctga ccaagccatg catgattttt atatgagatt
    10681 tgcccctcaa gtaatcgagg catcgggcag tagtgctggt ggtggaatgg gtgcaggact
    10741 gttagcattt gcttcaggga aaatagtctc tggcatcgac tacgtattgg atttactgga
    10801 gttcgataaa cgggtacagc aagcagacct cgtaatcgta ggcgaaggac gtctagatgg
    10861 acaaagtcta aaaggaaaag cgccagttgg gattgctaga agagtaccag agggcgttcc
    10921 tgtgatagct atttgcggaa gtgttggtaa aggaagcgaa aaagcagcag atgttggcat
    10981 ttcggccgtt tttccgatcc ttcctgcagc tgtgccttta caagaagctt tacagcaagc
    11041 cactgataat cttcatcgca cagctaaaaa catcgcatct ttacttcaag tcgttcattt
    11101 tcaaggaggt aatcgatgaa aatcaaagca gcactattcg atttagatgg cgttcttgta
    11161 gacacagctc gttatcatta cgaagcgtgg ctggttttag ctaaccagct gtccattcca
    11221 ttcactgaga aagaaaacga gcaactgaaa ggaatatcca gaaccgagtc actagagcga
    11281 ttgctgtcat ttggaaaaat ggaacagaaa ttctcagaaa aagagaaatc agcctttgct
    11341 gagcaaaaaa acaatctata tcttcaagcg attcaaaaaa tggatgaaac atccgttttg
    11401 cctggtgcaa tagctgttct tgagtacttg aaaaaaacaa acatcaagat cggattggga
    11461 tcagcaagca aaaatgcacg cctcatttta gaaaaaacga atttgacaag ttattttgat
    11521 gtccttattg acggcacgca agtatcaaaa gcaaaacctg atccagaagt ttttctcaaa
    11581 ggagcacagc aattgaatgt accgccaaat gcatgcttgg tcattgaaga ttcagaagct
    11641 ggttgtcaag cagctttggc tggtaacatg catgtattgg gaataggtga aaacataaat
    11701 cttccttctg ctgaatatgt gattcctgat ttaaccgttt ttgatcaagt acgttcgttt
    11761 tggcatttat cagaagccgt atcacgtata taatagtttc cgttttttct gtcacaagca
    11821 aaacgttatc ctccttttgg aaatcaaggt atgatgagaa aaaaggggga ttttatatga
    11881 caaaaaaagc agttttcgca ggcggatgct tttggtgtat ggtcaaacca tttgaagagc
    11941 agccagggat catttctgtg acatccggtt atactggcgg gcatgtacca aatccaactt
    12001 atgaacaagt aacaacaggc acaacagggc atacagaagc agtagagatc gaatatgatc
    12061 cagaagttat ttcttatgat cagttggtgg aaatctattg gcagcaaacc gatccgactg
    12121 acgcattagg acagttcgct gatcgcggtg attcttatcg tccagtgatc tactataatg
    12181 atgaagaaga acgaaatatt gcagagcgtt caaaacaagc attgcaagac agcgggcgat
    12241 tcgataagcc aatcgtgaca aaaatcgaac caagaagcga attttatcct gcagaagatt
    12301 accatcagga ttattacaag aaaaacaagc tccattacaa tctttatcga gaaggttctg
    12361 gacgtgcagg tttccttcgt cgacattggg gcgtcaagaa ataagaagcc gaaaaagcta
    12421 cttatggaac accgtatatc agaggttgta aaaccagcat tttgagctaa tactgagggt
    12481 cagctgattg aacaccattt agtggagagg ttgtgaacct gtcgcctagc tccaagtcac
    12541 aaggatttgg taagagaaca ctatatatca gaggttgtga caagtcgttt gtcacaacct
    12601 tttttcaata cctaagatga aggacaatca gcaacgcact gctcaaaaaa ggaatgaaag
    12661 gcagcttttc caactttttt ttatgacaga taaagtaaac agaaaaaacg gatattccaa
    12721 acagactagc aagaaacaag atctgaagca actcttctag agacaagcca ccactccaag
    12781 aaagcatcaa cagagtatcg ccagctccga gataggattg accatagcga aggcacacac
    12841 cgcctacgat aaacgttaac agcacacttt gccattggaa gacgccggcc tgcaaccaaa
    12901 aaatccataa aattgcccaa gaacagaata gcgtgtgaga atggaccatg aagtaaaata
    12961 aatccatcaa cgaaaatagc aaagccgaaa gcatccatat gaacgtcgaa aaatttcttg
    13021 cggaagaaaa agtaaagaac caggcaaaaa gaccaccaca taaggcttct gccaacaaat
    13081 agtagaaggg caaccgacac ctgcaatagc ggcagcgaaa tcgttggacg atgatcgaga
    13141 gaatcggaat caattcatac caagaaagcg aacgacagca tttgacacaa tgagatctag
    13201 gataaataat agagtgacct aacggaatcc gctgggcggt taaacataaa aatgatccga
    13261 tacagcaacc aatgataaaa taaagtaaca cgccatctcc cccttttcaa aaccaagata
    13321 cgcaagctta aactaaattt ttgtacatac ataaaatttg aggtgaaaaa atgaacgttt
    13381 caatcggatt agatatcgga accacacaaa ccaaagcagt tgctttcaca gatgaagccc
    13441 aagaagcagc atccgcttat tttcgctacc ctttgatcca agaaacacaa gggatggcag
    13501 aacaagatca ggaattgatc tttcaaggtg tctgcaccgt tataaaagaa gtaattaaac
    13561 agttgccttc agacacaatc attcaatttg tctccttctc ctctgcgatg catggcctga
    13621 tattgttgga tgaagagaaa acaccgttat ctcgaatgat cacttgggca gataaccgag
    13681 cagcggacga agcagaaaaa ctgaaatata ccgtgcttgg caaagaattt tatcggcaga
    13741 ctgggacgcc gattcatcca atgacaccat tgaacaaaat caagtggctg caaaacagcc
    13801 aacctgaatt attcaaaaaa actgcttatt ttgtcggtat caaagactat atcttttata
    13861 gattgtttgg cgaactgatc agcgactacg gaacagcttc aggaactgga tattttgata
    13921 tccatgaatt ccaatgggca gaagaagtgc ttgactactt gaacattcga atagagcaat
    13981 tgccgcaagt ctttccttcc acctatcaag taacaggtct accatcaaaa acagcagcag
    14041 aactaggttt gccaaatgat atatcatttg ttttgggagg ggcagacggg ccgctttcta
    14101 atctagggtt aggtgctttt ggtgatacga tcgccgcttt gaccgtagga acgagcggag
    14161 cacttcgttt tattactact cagcctcagt tccatccaga aatggagacg ttttgttatg
    14221 ttctggatca gaaacattgg gtagtgggag gagcgactag caatggtgct ggcatctttg
    14281 attgggcagg gcatacattt ttacaggaag tcgttcaatc tgctaaagaa aaaggagaaa
    14341 atccatatga tgcactgctt tcggctatcg cttttgtgcc agcgggtgcc aatggtctga
    14401 tttttcatcc ctatttactt ggtgagcgag caccgctttg ggacgcagat gcgtttggca
    14461 gttttatcgg tttacagcgg caacatacag aaaaagaaat gatgaaggcg attctagaag
    14521 ggatctgtct caacctttat cggattttaa aaggagtttg ccaggaaaat cagctcacag
    14581 agatccgtgc tacaggcggg tttgctcaat ctccgatttt tcgtcagatg atggctgatg
    14641 tactgggtta cccgcttgtt ttccctactg tcacagaagc atccgcatta ggagctgtga
    14701 ttttaggctg gagaagcatc ggaaagatcc aaagtatcag tgaagcacag gaattgatag
    14761 aattgaaaga gcaagttgga gtacaagaaa ctgatcataa aaaatatcag aaaatctatc
    14821 cattatttgt ttcgacacaa aagcatttag cacagactta tcagcaattt gctcaactgc
    14881 gagaagaatt agctgattct gaaaaatgat tggctttacg gataaaaaag cgaactgaaa
    14941 atcgttctca ggtaaatgat tttgttgtac ttttcattga aagtctacta ttattttaag
    15001 tgtaagaaaa tgttaggagg caagaaagat gaaattccaa caaaccaaag aagtattaaa
    15061 tcaattagtt gcagatctaa gccaaatgtc tgttgttgtt caccaaacac attggtacat
    15121 gagaggacca ggtttcctta cattgcaccc aatgatggat gagttcatgg atgatttgaa
    15181 cgctcagtta gatgaagtat ccgaacgctt gatcacatta gacggttcac cttattctac
    15241 attgcgtgaa tttgcagata acacaaaaat ccctgatgaa attggccgct gggatcgtac
    15301 tattccagaa cgtttggaaa cacttgttgc aggctatcgt tatctagcag atctttacca
    15361 aaaaggaatc gaagtttctg gagaagaagg cgacgatcca acacaagata tctttatcga
    15421 attcaaaaca gccacagaaa aacgtatctg gatgatccaa gctcatttgg gcaaagcgcc
    15481 agagatcgac gcgtaaaaac tgttgttgaa cttgaccgaa aaagaggttg tgaaatcagc
    15541 gttttgacct gagtattaga gaaacaaacg tcaaaatagt tcctcatatt ttgccgtttt
    15601 ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt gtaagcctgc
    15661 cgttcagctc cgagtcacaa gaaacaattt taaaaaacag cttcttatgt tttatggata
    15721 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcaaaat aaggggttgt
    15781 gacaagactt ttgtcacagc ctctttttgt ttttcatttt gacaggaaac gaccctttta
    15841 tataaaagaa agaaaagaag taaagtttca tataaatttt tcaatcaagc agaaaaacgt
    15901 tgacattgac tagattagcg ggtaatatgt taaatggtat tgtttgcccc acaaagagcc
    15961 ccggaaactc gaagtgtatg tcaaacatca gaacttaaat tggaggaaaa taacgtgcgt
    16021 acaacatata tggccaaacc aggcgaagta gaacgtaaat ggtatgtagt agacgcaact
    16081 gatgttccat tgggacgtct ttcaacagtt gttgcatctg tactacgtgg taaaaataaa
    16141 ccaactttca cacctcattt ggatactggc gatttcgtca tcgtcatcaa tgcagacaaa
    16201 gtgaaattaa caggtaaaaa agcgactgac aaaatttatt accgtcacag caactaccca
    16261 ggaggattga aatcaatcac tgctggcgaa ttgcgtgcta aaaattctcg tcgtttgatc
    16321 gaaacttctg taaaaggtat gcttcctaaa aacactttag gccgcaaaca atttacaaaa
    16381 ttgaacgtat acggtggagc agaacatcca catgcagcac aacaaccaga agttttagac
    16441 atcacaaact taatctaata ggagggaatt tcattggcac aagttcaata tatcggcaca
    16501 ggccgtcgta aaaacgcagt tgctcgcgta cgtctagtac caggaactgg caaaattact
    16561 gtaaataaaa aagatgttga agaatacatt ccacatgctg acttgcgtga agtaatcaat
    16621 caacctttcg ctgttactga aacaaaaggc gcatatgacg tatttgttaa cgtaaacggc
    16681 ggaggatacg ctggacaatc aggagctatc cgtcatggta tcgctcgtgc attgctagaa
    16741 gttgatcctg acttccgttt agctcttaaa cgtgctggct tacttactcg tgacgcacgt
    16801 atggtcgaac gtaaaaaacc aggtcttaag aaagcacgta aagcttcaca attctcaaaa
    16861 cgttaatatc ctttatatat caacgtttct ggcacttttc aattatttgg aaagtgcttc
    16921 ttttgttttt aagaacacat ttaagaacac atatcaatca cacttattca atcctctctg
    16981 aaaagcttca aaaacagcct tatcaactat atctggtgtg ttcacatata tctttgttgt
    17041 agaaatatct gagtgtgtta aagcatttga aatttgattc atatctgctc caccttgtct
    17101 agctatagtt gcataagtat gacggagttt gtgtggagat aaatgaacta agtcaggatg
    17161 ccttctttta attgaattga ttcgatagtt taggtaatcg gcatgaacag gacaatttac
    17221 ttctccagat ggtttagtat aagtaaagag atactgatct aacgtttgct taatttttaa
    17281 tttcaacaat tgatcagctt gtaccgattt ccattcactt agcaatgtca ttacaacttc
    17341 tggtacttga atgacggtat cttttcgacc ttttgtaaac ttttttcttt gatatttatc
    17401 taaagtatgt ttaagacgga ccgtttgttt ttcaaaatca atatatttcc attgcaaagc
    17461 gtaagtctca ctttttctat caccaagata cagggtaatc ataaaaagtg tgtaatcatg
    17521 aaaagtaagt aaatgattat taaaatcatc atttactgct tctatccaat tagtaagttc
    17581 ttttgatgat agtgcttgct taccttcttg tattcttttt tcttctaaag catttttttt
    17641 aggtgcagtg atactttgaa taattttagt agttcgatta aattcgatat aatttagtat
    17701 ttctgcaata tcgaacatgg aacgaacata tccttttatg atttttatat ttgcatattc
    17761 tttcgatttt ttggttaatt catctgatat tatctcagta ttgatattta gataattcat
    17821 tgcgtattca ccaaacatag gtaaaatatg caaccgaaat atatcttttg tattactaat
    17881 ggttatatca ctaggaggcc tgtttgatct aattgtttga cctaattcat atctaggaag
    17941 ccatttagat tcgtaaaatt ttttaaacgt tatttttcct tttaattcta atgagtttgc
    18001 attctcctct ctcaaaactt tttctatttt cttcttcata tcattttcgg ctttaatcgc
    18061 caattgccta tttggaaatg ttttttgata cttttctgat gcaattcctg ttatttcttt
    18121 aaaccttttt ggatatctca gattagtttt atatttccca tttgaaagct ttgtaggcaa
    18181 aatcctcact ccttaaaaaa agtaaggcag tatatttcac taattttagt atatgcggcg
    18241 agaagaaaat ttcaagtatt cataaagtct ttaatatctt ctacgtcaaa gcgaactaca
    18301 ccaccaactc ttattactgg gaaattatat atacgaatcc acttatctag agtattatta
    18361 tcgattccta gaaatgcaca ggtttgtttt ttattcaggt agactctgga ttctataaaa
    18421 tcatctccta aaatagtagt tatgaaattt ttgaattgct ttgggtaagc acataggaat
    18481 ttcacctctc cccagtttac ggcgagcctt acggaagtat cattatttgc tgctattgtc
    18541 atggcatttt gttttcccag aacaaaaaga aatctctaag tatttgtcgc taatcaggtc
    18601 atggcgtact tgagtttcta gctcgaatag cagggatcgt tattcaaaaa ttgtatttaa
    18661 ttgtcaatga tcaaaaatag tttttttcct att
//
LOCUS       DAZKMZ010000047        18616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000047 DAZKMZ010000000
VERSION     DAZKMZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.047"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..913)
                     /locus_tag="ACG8M5_001767"
     CDS             complement(2..913)
                     /locus_tag="ACG8M5_001767"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0359019.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1290..1928)
                     /locus_tag="ACG8M5_001768"
     CDS             complement(1290..1928)
                     /locus_tag="ACG8M5_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359020.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1932..3914)
                     /locus_tag="ACG8M5_001769"
                     /pseudo
     CDS             complement(1932..3914)
                     /locus_tag="ACG8M5_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4094..4759
                     /locus_tag="ACG8M5_001770"
     CDS             4094..4759
                     /locus_tag="ACG8M5_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359021.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5156..7189)
                     /gene="brxL"
                     /locus_tag="ACG8M5_001771"
     CDS             complement(5156..7189)
                     /gene="brxL"
                     /locus_tag="ACG8M5_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0359022.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7307..9829)
                     /gene="pglZ"
                     /locus_tag="ACG8M5_001772"
     CDS             complement(7307..9829)
                     /gene="pglZ"
                     /locus_tag="ACG8M5_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0359023.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9945..13577)
                     /gene="pglX"
                     /locus_tag="ACG8M5_001773"
     CDS             complement(9945..13577)
                     /gene="pglX"
                     /locus_tag="ACG8M5_001773"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0359024.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13641..17312)
                     /gene="brxC"
                     /locus_tag="ACG8M5_001774"
     CDS             complement(13641..17312)
                     /gene="brxC"
                     /locus_tag="ACG8M5_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0359025.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17337..17912)
                     /locus_tag="ACG8M5_001775"
     CDS             complement(17337..17912)
                     /locus_tag="ACG8M5_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0359026.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17912..18511)
                     /locus_tag="ACG8M5_001776"
     CDS             complement(17912..18511)
                     /locus_tag="ACG8M5_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0359027.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5736 a         3559 c         2608 g         6713 t
ORIGIN      
        1 cttatttact acttgctgta ctagaatctg aagtgaatgt actcaaaaca ttttcaaagt
       61 ctttgtcttt gatttttaca ttagcgtctt tcaatacttc gccaattact ttattctgga
      121 aggtactgtc acttaatttt gtgtctgttg caatttcttt caattctttt ttgtatttat
      181 ccatgtcgtt gcctttgttt tggtttttca ccattttaac cacataatat tccgtagtat
      241 aagttgaagc attggtagaa gtgatcacat cagaaacttg accatctttc aacttaaagg
      301 ctgcttcttt gacttctgcc ggaaccgttg tagtagtgga atcaaatttc acttttccgc
      361 catcttcttt tgtcgtagta tcagttgact tgtctttggc taatttagag aaatcgtctc
      421 ccttatcagc agctttcttc acatcttttg catcatcttc acttgctact tggataattt
      481 gagcttccac ttctggatgg aaagattccc aagccgtttt taaatcttca tcggtcaatt
      541 tgatatgttt ctttagacct tcttgaaagg ctagactttg tttgatactg tctttgaatg
      601 tttgttctgt gtaacctgca gatttcaatt gtgaatcaaa tgaatcaccc aattgtttct
      661 ttgtttgatc atattgggaa tcgatttctt tggttgatac gtcatctcca tatttatctt
      721 caaatacttg ataaacgatc aactgttgca cagcggaatc tgctccttgc atttttttca
      781 agctatccat tacttcttgt tcgctgattg taccgccttt ataactaacg gctgtttttt
      841 caccggcatt cgtacagcct gacaataagc cagtgcctag tacgatggca agtgctaaaa
      901 atcttttttt catttatcaa ttctcctact tttcgttttt tgcgaattgc taaaaacaaa
      961 ggtgttcaag atgaaaatca tcaagaacac ctttactaat ctgctcgata cgtcctgaat
     1021 agatataagg ctacacaatc cagaaagttc gctcagcaga ataaatgtca agtagcacat
     1081 cacaccttaa tatactactt cttttgttac cgtttttcaa gtctatcttg cgttccgtat
     1141 ttaacgtaac taattcttag aacaaatgtt atcgattttg tttttcttct tccgctgttg
     1201 ggatattcaa atcagacact tttatgttcg attttccaga catttttttg ttgtttttat
     1261 tagaaaaagt ttgttttttc atttgttttc taaagcatga tcttcttgtt tcctattttg
     1321 gtcagatatg gatcatgcgt gaccacgata actaacgtac cttgggaagc ctgttgcgat
     1381 aagatttcaa cgacttgctc cttatttcgt ttatctaatg atccagttgg ttcatcggct
     1441 aaaatgattt tgctattctt caataagact ctcgcacagg cgattctttg cttctcacca
     1501 ccagataatg tgtagatagg cgtacttagc ttcttgttta tatttacttt tttcaatgcc
     1561 tcatactttg ctgttcgctt atctgaaaca tttaaattcg aaaacttcaa tccaatatct
     1621 aaattaaaat cgattgtttc atattcaatc aacccaaaat cttgaaaaag aaaagagagg
     1681 gcatttcttt ttatcaagcg cttctctctt ccagttaaat gttttgtttc ttgattaaat
     1741 aaataaaatt gccctgaact gaattgatca ttcaatccta atatattcaa taaagtactt
     1801 ttccctgctc cgctctctcc aactacagtc acaaaatccc cagcatccgc agtgaaacga
     1861 tccaaatgaa gaatcgtttt gtttcgaatg atttttttcg caccttttat cactatcaac
     1921 ggctccaatt gttaacctcc attcatcagc aataactgac ttttcttttc tgtagacaaa
     1981 ataaaaacaa ctgaaagaat aaaatcaatg aagctaaaga aaaaaatatt aaaaagatat
     2041 ttaggctctc tttctataag aaaaaggaag attacacttg tcgttcccca tttgatgagg
     2101 ttcatcatga agaaatcttt atacgtttca aagagcgaat aaccacttga acgaaagata
     2161 gcgattttcc gacaattgta gacaaaatat atgcataaca tgacatagct caacaagaag
     2221 aaagaaaaga aagtgagtaa aaatatctgt atcaagtaat gaaatgatct gcttaacact
     2281 ttgacagcta tctcttcagt ctgagtatac ggaaatgata actgcaagtt atcacgtaac
     2341 tgactttctt caatcagctc aaataacctt tctttcgaca aagagcctat cttgatcttt
     2401 aacggtggat agatttctcc gctgagaata tttctttccc aatacgtact gtttttcaat
     2461 gtcagtattt caacaaccgg ataatgttcg atccagggga tagaaggaat aaatgtataa
     2521 atgggttgat tgttttttat aaatagaaat ttcggttctt tttcactgat ttcttgataa
     2581 tattctgtta tttgcgccag gtcatttttt aaatctgtat gtctatatcg ttcagggatc
     2641 aatataacag ggtgcgtttc tttttcggtt atttggatca accttccaga gacatcaatc
     2701 aaaggaaatt tcttaagata attcagattt atttttatcc ctctgccaaa ctgttcattt
     2761 tcttcattca agtaatctga tacattgact aatatacttc cgtttttatt caaagtttca
     2821 tataattcct gatcggctgc tgatgcaaaa cgattggaat aaataaaatc cacgtggttt
     2881 ttcccgatgg ttactggata aaatacatga tagtcctcac cagcgatttc ttttggtgtt
     2941 tcatgggaaa cagctaactg tgaagaagaa aaaacgatag ttgctaattc gacattggca
     3001 acggtcaaaa gaagagaaaa caacaatcca aatgtaaaaa gaatgtattg gatatagttg
     3061 aacgcttctc ttaacgaagc gattttttca actaataaaa gagaaatcaa agagataccc
     3121 tatattccgc tgaacaagaa aacgtacatc tggaacaaat aaagaaattg ctccttataa
     3181 tacataaaaa taaaaccacc aatgattcct gcaattatga atgtaataat gattttcttt
     3241 ttccccaata aacaccacaa tacttgatgt aatgaaaagc cattattcaa caataaactg
     3301 atttctttat tcatcaataa gaaaaaggat gataacaaaa ggaataaaag aatcaatcca
     3361 atagttaaat acactcgata attatatacg tctgtacttg aaataaccga atacgaatag
     3421 ctattttcaa agtcttcaaa aatattagtg gcataatcag tgtgaaatgc ttgattgaat
     3481 cgttccacaa atagtaaaat gaaccgttca taggcttctt tttctctcgt catcaagaaa
     3541 aattcacctt caaaatcttt tattttgatc gattcttcaa tggacgttac gcgaaaggag
     3601 gtattagaat agggtacctt ctcgtgagaa gatttgacat ccccttgata atacaatgtc
     3661 atttcaagct ttggctgtat cttgaagttc caagatgtat ctacttcatt ggattcattt
     3721 actgtttttt tcatataaga aagctcagtt tctgatgaga cattttcaaa taccatgatt
     3781 agctgctcat attcttcttg cccaataaaa tgttgtggca atcgaatcgg agtataagta
     3841 tctgtttgat ggaaaaattc atttgtccga tagagtacct gtttatttat aataaccaag
     3901 gcaaaaccac tcatgatacc ggtcactatg attgtgagta aaactgtgaa cttttttttg
     3961 attatcacca ctctccctta cttaaatgat tctttgataa aatcataccg tttttcatca
     4021 aaaaaacgta tctatcaaat gtataggtca tgtagaataa atctatatac tgtagaatcg
     4081 aaaggagaag aagatgaaca tcggagaaaa aatacgaact taccgcagaa attgtaagct
     4141 tacgcaaaaa gaattaggcg aaaaaatcgg tgtttccgat aaaacaatct ctagttggga
     4201 aaattctcgt acaatgccag accttgaaat gctgtcactt ttacatcaga ctttaggtct
     4261 gccgattgat tttccttcta ttgaatcagc taccgaatca atagaaccaa cattgccgga
     4321 tgaacctctt gttatcaaga aatcttattc atcaaaacaa cccttctcac tcaaaaagac
     4381 actatctttc tttctgctcg tcttttgttt ggtctatctt tttcttatca attatcaata
     4441 taaaaatgaa acggttgatc gattcaatcc ttttttgaaa ccacaaatcg gctataccac
     4501 actccctgta ggatacgtgg aaaattccat cgaacattgg gtatgtaatg atatctttgt
     4561 tcatggtaca tggctgtctt ttcacgctgg agaggtagaa aaaggtaaga atcatgctgt
     4621 aatcaaacac aaaggaaaac acgtcatgaa cgttcgcctg ctcacttggg aagccattcc
     4681 ctcagattac caagaaatcc tcatccaaga ctatgacgat gccttaaaaa gaagccctca
     4741 ggagccagat aggcattaag taggagatga acgtagagat agaaaaaatc aatgcacatg
     4801 atcttatcaa aaaaaaacag gggtacaaat taattgaaaa agctttgaga tgaaagttcc
     4861 taaataaaaa agttcagaat gataggtaaa aaaatgacta actattctag tattaggcta
     4921 tttgtattat tcttctgtta tttttatatc aaaaaatcca gaaataacta aatataggta
     4981 tttctggctt atagtataat atttacgcca cagcccccaa tgattctttt tggaatcatg
     5041 atcaaggacg gacatacaca aaaatatttt gcccattcga tccgaataga ttaacgacta
     5101 atctagtaag taatatagat tggtacgtag tcaaaccagc aaatggaaaa gttgtctatt
     5161 ccaccccaag ggctttaaag acagcatctt ccggcgtttg ataaaaaatc aaattaaagg
     5221 tacccattaa ttctgggggt acagttccaa tatcagcagc gcttatcata ggtacaagta
     5281 cttttttcgc tccggcatct tttgctactt gtaaacagtt agctaaatta tctaccttaa
     5341 tcatagaacc accaattgta atatccccta aaataactaa acttggtaat acaggtttat
     5401 ttaaggaaac tgagcataaa gctattatag ttgataacga taatgttttt gtcataccaa
     5461 tgccattcat atcttgaaca tgaacgacaa aatcttttgt cgtcaaacta atagactgac
     5521 taacacttcc ataattagct cttaaatatc ggtatgccga atccatcgct tctcttgctc
     5581 cagcatctga attgatccca gtacgttcaa actttccatt tcccgaagtc atttgcgttt
     5641 ccaatttata ggtacctaat tgcccagttt tgccataaga taccgtgtac aaatgccctg
     5701 gcttccctaa cccttctgga atcaaagttc ctccaccttg ttcaggaaca gaaacatact
     5761 cttctcgctc ttcttctaaa tcaatgtatg aaaaattgac atcataaaat tccattccac
     5821 caattttctt taattgttct tttacacgac gacgcatttc aagagcaaat cttagtattt
     5881 cttcgacatc acttttttca aatctaccat caggatacaa cagtttcatc atcccagata
     5941 caatccgacg aactgcaatc gtatcccgct gatttaattg agaaccaagt ttgaaatatt
     6001 tatcaaaact atcgccaaaa ctaacttttc gaagctcacg catgacttca gaaaaataat
     6061 ctgtaatgaa gccatagtca tttgtaaaaa aatctggtcg atatttagga atttcccaac
     6121 ctggtaaata acaatgcatc cgatccaaaa aggctgaatc cgtccccata acctccggaa
     6181 atggctcaaa taaattcgaa gttttcaata ttacatcaac gctttgatta atatttccta
     6241 caaaagccat tgatgctgaa gcattctttt cttctttgcc tctagcaaaa gatcctgaag
     6301 ccatgtaatc tttcatgatt tgaacgccat cattatcctt aaatttaatc ccagcaactt
     6361 cgtcaaaagc aacacagtcc cacataccta caagtcccat tgtacgacta gccatattat
     6421 agaaaaggtt tgctactgtt gtttgtccac ctgaaacaag gattgagttt ggtgaaattt
     6481 ctttatatac atgcgattta cctgtaccac gtggtcctag ttcacagaga ttaaaattat
     6541 tttcaattaa tggaataagt cttgctaaat gaagccattt taccctttta ttaaattgtg
     6601 taggttccat accaatggac cgtagaagta agtcaatcca ttcatttgta gtaaactcct
     6661 tgcgagccat cttgtaagcc tcaatgtcta aaccaggcat ttggattgga tcaacctttt
     6721 ttgcaataaa agggctattc caacgatcat tctcatcata ccaatattcc atcgaaacaa
     6781 tacaccagat ccccccacct aataatcgat caaatttttt cggatactct tcactaattt
     6841 gaacattttt taatcccagg ttagagaatt ctgcttcata acaatctttc ttttcattta
     6901 aatgtacagt tactttatca ataactgtat aaccacccaa ttcacgaatt tttgctttta
     6961 ctttttctgc ttcatcagga cgcacatagt ttcgagcaag aatattttta acattttcca
     7021 cacctttttc aaccaatgtt tcatccaatg acgaagcata cataccaagt aaatattcca
     7081 atacataaac aggtacattc gcaccttctt taattttctt tgttaaatct tttctgacaa
     7141 tctttccggg aaagaattca gataacttta aaatttcctg ttgttccact agaattcctc
     7201 catgtgttca gtcattataa tagcaattaa agtatatcat acttaaccag taggcttgat
     7261 aatacaaaag aaaaagtatc cacaaaaaat tgtggatacc tcgtttttat atatcaaaat
     7321 caaatgattc tgcaaaagga ttatctataa tgaattttat tcgctcggtc aaatcatttt
     7381 tttgattttt tatcactaaa taataagtat cttcaactgt atatcgacga tttacaaatt
     7441 caaaaacaaa tttagtaaac cgctgatttg catcttctgt ttttgaatcc gcaacataga
     7501 tttcttcatt tgagatacgt tcaccatatt catcttcaaa ataaagccag aaagatgcgg
     7561 ggtcataaac atcactaatt gcattttttt gatagaattc taacgtggtt gataatcctg
     7621 taattctgcg gttgctagtc attaattcaa ttgctactgg ttcctttgca ttgctacctt
     7681 tggctaccgt aatttctaga acagggacta gcatttcttg aatagatgac cctccatgaa
     7741 cgtagctttg tcccccgcca gctttcttaa aaatacttga agtaatcggg acacaaatgt
     7801 ttctagtgtc atcatttcct aaagaatctc ccaaagagta atttttcgta ccaattattt
     7861 cgtattcttc ttcactaaga ataaaacgac gttctacacg atctttttca tttctagatg
     7921 gattttcaat cttatctact tcttcaacgt ggcttctttt ataaataaat ccatgatcag
     7981 ccgtaactat aaaacgatag atactgccat cattatgcaa gcgattaata agtcgggaaa
     8041 tttcgcttat cgctaaatca caggcctcaa aaactcgatt ttcattcgag tgatcacctg
     8101 ttctatccac ttgatcgtga taaatataaa tgactgattt gccattaaat acttctcgta
     8161 cctcagcctt attcatctta accacatcat cataatttaa agctacagca tttgcatctc
     8221 gttgctggag aatcgcattt cgattggata aaccattcgt ttttttccca tcaatcagta
     8281 cttctgttcc ttcattgtaa gttagttgat tgtttggtaa taaggcggct ttaccgaatt
     8341 cagtaactga aggtaataca gaaagaaagc tagccatgtt tgttgaatac ttcttttctc
     8401 taactaactt acgttctaaa ttcatcccta actcatatcg aaaagcatct gaaataataa
     8461 ctacagtttt aacagagcgt ttcattacag cttgattata aaattttcga atggatggac
     8521 gattcttaaa ttcaaaagtt tgattccaaa tttgtcctga ttgatcaaga aacttcgaat
     8581 aatttgcttc aactaatcga tacaattggc catatttttg cttttcttct atatgatcac
     8641 tattccaaat aaatttccga taatgcgtat caacccaata catttccttc tcatattgag
     8701 ttagcaaatt atccatgttc atcggcaagc acaatctgtt tttatccaca agataataag
     8761 catgtttcaa taattgatac tcataaatat aatccgatgc gaaatgcatt ttttcgcgct
     8821 gctttaaact atcaaaaatt gtttgtcctg caattttcgt tgtagtgtca gaagccttta
     8881 atcgttctgc ataaaacatt aatatttttg catgaatctc agaaaatacg tctgcttcaa
     8941 ttagatcatc aatttctata ctgtctaata atccatcacc atcaatagcc tgatatactt
     9001 ttttagatag ctgttcaaag ctttccatgt aacgtgtatc attcataaac tgatccatga
     9061 aagtaataat gtttgctttc aaatgtagta tttcaaatcc tttgaacttc ttcggcatct
     9121 cttgatcaat ttgataataa aaatagttca aataaagaca catcgtcagc ttttccaatg
     9181 ttggctggct atccgtaaag ccaaatttca tttgaataaa ttcccaaaat ttagctgtta
     9241 actgatactt atcaagttcc cttaaaggat tctccctact ttctgtaaag ctttttagaa
     9301 ctacacgcag taaatcattt aatcgaatac ttttcgattt caacaaaaca gccattatca
     9361 ttaattcaat cgtttctgat ttattaattg tcatatgcgt actaaattca ccaaaacttc
     9421 tcatgcgatc ctttgcatta aagaaattac gatactgttt aactgtctga tgaaatctaa
     9481 gtggaatagc caattcattc atgataatcg ctaagcggtc tgcactaaat tcctctgaat
     9541 aattcaatac actcaacaaa tgattatctt catctttatc atcccaacga ggaaatggcg
     9601 catagattaa atagttagtt tcgggatctt catattccaa taaacgctta gttttaaatt
     9661 gtgcgtcctt ttctaatcga tagatttttg cattttgaat gaccaatgag tcaatttctt
     9721 caagaaaatc ctgatttgaa tcaaaccaaa aaatgatttt cctacatcca taattaggaa
     9781 aacgttgatt taatttttct tcaatttgtt gcgtatttat atctgtcatc aatctcacct
     9841 aatcgttctc tttctatacc ccttatattg acataatttg gataatttcg cacgcaattt
     9901 aagaataaaa aagaaagttg ttagaaaaca actttcttag ataactattt ttttggttct
     9961 ttgacccctt ttgcaaaaat ttggaatttc tcgccggttt taggatcacg ctgcaactta
    10021 tcataattga caattacacc atcatctaag tctaattcaa ttcgttttaa tgctaagtga
    10081 cccaacttct cgtcatactc acgaacttct tttaattgct tctgaatctt tacaatttct
    10141 tttgtcattg catttctttc ttttgggtta tcacttgttg aaatttgttg ctgacgcaat
    10201 tcgattcgat tgtcatacgc tttttgtagt tcgtgtaggt attcagtacg tacttttcct
    10261 aattgatctg gtgtatagcg atgcaagtac attaacgctt taaagccatt ttgttttcct
    10321 gagtccaatt gccaataaat tggtcgattg gagtatgttt ttaaatggtc tttgtaaaaa
    10381 tcttgtacga agtaacgacg aataatttct cgacttgtcc ctttgccacc taaagtgtca
    10441 gcgataaact ggagattttc ttcaagggtt tctttaccaa aagtaatctc aacgaaatca
    10501 atgaaacgat tgacaatgtc aatttggcta tccttgaaat actcatcatc ggtgatgagt
    10561 aaaacattat cagtatcagg tttaaatttt tgatagcggg aaggatccca ttcgccgcca
    10621 gcgaagatga gaccatcttc gtctagggag taacgaccga agatacaacc tattgaataa
    10681 gaaataaaat ccttaatttc cctcgcctta tcaggtaacc ttgcagagat atattcatct
    10741 tgaacctctt ttgttaattc tttttcaaga gaataatgat taatgaatat gtcgttcagt
    10801 tcctcttcat tcgattttag agtattaaat tgattaacca actgcctact tagacagtta
    10861 aatgcctctg agagatcttt tttaaacata taacttaaca aaatgtttct tctaaactcg
    10921 tatgacgttt caagagcatt atagtctttt ctagctatat cgatattttc atccactttt
    10981 tctcttattt tttttaattt tctctcagat gtaactttaa gaaaaggtag ttttcccatt
    11041 ggtccttcat ggtaatccaa ggtgggagaa ataaaagtta aaaagtgatt aactaattta
    11101 gagtttacat agccaaatat ttcttcaaag tcagttatat cctttgagaa cagaacagat
    11161 cctttagttt caaaaattgc ctgattctgc aatctaaaag atacttgtcc agatgaaagt
    11221 gtcgaccaag taattccctt tttaaaatag taattaagat tttgtggtct agattttaac
    11281 ttcccatttt cattaacata attttttatt tcatctccat cattctccca gttaaccacg
    11341 tattcattgt ttccatacca ttttctaaaa cgaccacctt tagttgtagg aaaccatttt
    11401 tttctagact ttttagcgtc ctctaatgtt ttagcatcaa actttatttt gttaaattct
    11461 acttcaaacc aataccgcaa aaatttattg ttatttccgg ttgccaaccc ttgttttgca
    11521 tctgcaacat cgttagttaa ttttccttga gcaaaatcag tgattacgtt atcgcttgcc
    11581 cagtaagcaa tcggcattcc tgggattttt ttgaagttgt cttgattagt actataaaaa
    11641 taatcgcatt caccatgctg atgaattgct tctaacgttt taattttttg aacttccatt
    11701 ccacctttaa aatctgataa ttttatatag cttccatctt gttcatttga attttttatc
    11761 gtaaacgtac aaattggaac ggttgcttct tcaaaagctg aatactccat ttgaatcaaa
    11821 ctagaaatgt ttctttcatt aattaaaaat gagcgtaact tttcatagct agaaatgaac
    11881 atccaaacaa atggcgtcat aaaggaagca tatccattat caatcagcat ttggctattc
    11941 aatttaataa acacagaaaa tagatctttt ttgacatcag aatagttatc atttacatat
    12001 ttttttaatt tcttatccat tttattaaga taaggcggat tagtgactac cgctacatat
    12061 ttatcaacta gcatttcagc cacgtcaaga atgtgcaagt atttttcttg tatttccttc
    12121 atcccataaa gaatatccat attcgtaaat tcgttgaatt ttgatatata ctctcttact
    12181 tttcctaagt attctttagt tattgcctga ttatttttat cgactaactt aatgattgat
    12241 ccaagttctt tcccattttc aaattgatca aaaattaact ctttgtattc attccagcgt
    12301 tcactatact tatcattgtt caattcacta atacgctctt gatactcgtc tgtcacttta
    12361 ggagaatcaa taaactctct taaattcggc gatagacttt ttttcaaaaa aagacgatcg
    12421 tattgacgcc ctttcatcat aattgcaaaa taagacaatt gatacgctct tttatcgatt
    12481 tcaagaccat ataaattgta ttcaacgatt gcacgtgcag catcacgctc agaatatcct
    12541 aacgtaagat aaatttccat caatacatcg aaggcgtaaa ctaaaatatg cccagacccc
    12601 attgcgttat cgataatttt tagttcttct ggttttaaat tacttacaga tgttgcttgt
    12661 tcaaaagcat atttatctgg aattaagtat ttcaacttat ttcttaattc actattggga
    12721 taatgatcta accaatactt tcctaaacta ttgtcaacca tataacgaac gacccaatct
    12781 gtggtaaata gttgagttgc tgccggaata tcttgttttt tgacaatccc tttataaata
    12841 ttcaccactt gattatgtgg ctcactatta tagtactgat acaaccaccc aataatctct
    12901 acctgtccac cagcttgaac atagaaatca tcctctggaa tacttaaaag atcagcgatt
    12961 acaccatctt ttttaatata agaagctgta aacaaaagtt ctgtataatc ttctacttct
    13021 tcaaatagct caggtaagta atcattcaat tgattacaga tttttataaa aatcaattga
    13081 tacagcttgt ccattgcttc tgctgaatta tctgttttcc aagttctgac ttgttgctgt
    13141 tcattactag ataattcttc aaagatatcg gcatcataaa tatctgtaat aatatctggc
    13201 tcaattttcc cacttatttc actagataat acacgatacg tgtcaggtaa ataatcattt
    13261 acctccataa accgaatggc aatcaatcgg ttaaaccacg tataagcaac ctcttcgatt
    13321 aactcttgaa aagcttctga ataagaagtg acagaagcac gttgttttag ttctgcaatc
    13381 aatttgtttc tttgcgctat ttcttttcct tgaattctta ttcctgattt ttcgatttct
    13441 tgtaaattgc tatcaatttt cttcacatct tcaatttttg attcatcaat tcctaaacgt
    13501 tttgctttag tcattacttc atcaattaat tttctacggg cattgaccgc gaaattcttt
    13561 atcgctttct tatccatttt tctttccctt ctatatgtaa cctttgaaaa gacaggcacc
    13621 tttttatgcc tgtcttttca ctagaattga atcgtgattt ctgtattttc ttgaagttgt
    13681 tccttcagtt tttgattaat ttcagctgtt aactcatcaa tatctgctgc tgacttaata
    13741 gttttaacat gaacgttaag tgttctcatt ggtactgttt tctgttgaat agtaggtttt
    13801 actatcgcta tttcttcagc ttttgctgca gagtctattt catctttttc agtttcacga
    13861 atcaagtttg ctgcttcaat ttgtttacgt gcttgttctt cttgacgtct gcggttttct
    13921 tcattatcga aacgttgatt aaagatcaaa tactgttctg ctacgtttaa tgataagaaa
    13981 acaagatcat ctatagtatt cgcttgttca atttttgctt tcagttcttg aaattcttgg
    14041 tcaactattg gtacaaattg atccttaaaa tcttttcctt ctaataattt aaatgttgct
    14101 tgtttttctt ccgttaattt atttaatatt tcctgacttt ccaactctaa cagttgatta
    14161 tattgttcaa taaattcttg aatcaactca tttagtttca tcatatctga ataaggatca
    14221 gcctttttca aaatggtttt catctcatga atcaattgat caatttcttg gctagaagtt
    14281 tgacgccgac ttttttcgat taatctaatt ttatcttgtg cattctccca gatttttttc
    14341 tgagtactat tttgcttgtg gaaattttta attttatcat atgtttcagc ccagtcgaat
    14401 aattcatcgc gatatttttt gattgtataa aaaaagtctt caacatctga cgtttgaaca
    14461 attacttcaa gatatttttt tatttgctca tattcataag cactagggta ggtataagtg
    14521 ttttgtaaat caaaataaga gcgtaaattg tctaattgac cttttaaata tttcttgaaa
    14581 ttttccattg tatgttcatt gttattttca tcaacaatat tacgactttg aaacagttct
    14641 ctaccgattt cgtcaaccaa tttttttgct tttggcgaaa tcaccgcgct tggttcaatt
    14701 tttatctttt cactttctcg tcgtttttga ataatcatta caatactatt tggatcatcc
    14761 atccgtgaaa tttgtacatt gttataaagt aaatcaattt taccagacat gaaaagttta
    14821 gcgatgcacc attccacatc aagttctgtg aatccatacg gttgttttgt ttcaaaacga
    14881 tcacgaattg ttttcatcgt aatcgttagg tgatttctct tttgatcatt gataaaattg
    14941 aaaatttcct gaactgcttg ctcatttgat ggatcttcaa taatttcatt tttgctttct
    15001 ttcactaaat ataagatatc ttctttttct ttaggcgcat caatttggtt taatttatgg
    15061 tatacttctt cagccatttt ctgaagtgct gattggaatt ttgctttaaa gtcggagcct
    15121 gtcagttgaa tttctgcacc gttatagaaa aattttgcag atttcaacag ttcatttaat
    15181 tgatcgttga tacttgcact tagttcctgt aattcaccag atttttccga acgaatcgcg
    15241 tcaaagttct ttactacact cgaataagtc gttgttaaat aaagattcat tctagcatac
    15301 attgctatat catctttaaa ttctttatct gatggcaatt ctacgaataa taagttacgg
    15361 gaactcttaa gcgcaagttc tgcctcttga cctgctaaat cagagtatgg tgaaataaaa
    15421 ttaactgtca tttcagcatt tccagcttgc ttgtatggac gttgatctaa cacttgtgca
    15481 taagtaaacg tataccggtt tttaaattta tttccacctg acatgcctaa tttttcatag
    15541 gcaaaactat ttcggtctag aacgccgtca aaaattcgtt tagatagttc tacgatcaaa
    15601 tcactatctg taatattttg atttttaatc tcacgagtaa tttcctgttc ttcatcagtt
    15661 tggaaaatat aactatctaa gttcttccgc acaaagtttt ccttaattaa aaggtccaat
    15721 gcttcttcta ctcgtttttt agtttccagg cggtcttcgt tgatatttct tatcatcaag
    15781 ctcgtaatat tttcaacatt tggtttgatg gcttgatcaa catatttaat catgaataaa
    15841 attcgtaaga catttacgtt aaagttatct atatcagttg gtctttgaat atgagaatta
    15901 tttgtagctt gtgtaatcac tcggctaatt gctgtatcaa tccaacgttc taacgagtaa
    15961 taaaactgat ctaaagaaac caaactggtt gtatcatttt ccttatttcg ttcagcggct
    16021 tctttaaaca ttgctagtaa tgaacgttca ccagacgata agtttgatcc ttgatagctg
    16081 tgattacgaa tctcggttag aatttgcccc aataaattga attgataggg aataaatgga
    16141 taaacttctg cgtaattttc agcatcttgg aagagcttta attcaggact atcaacaaag
    16201 agtaatttat ttttcaattc tgtttgaaca gtttcaaatt gtaaggctaa agaatcattg
    16261 gcaaactctt ttttcttcaa aatacgttct ttaattactt catcggcatt tgctgaagat
    16321 aaactcacag ttgtggcaaa acgatccttg atcttagaaa aatcattatc agaattgtta
    16381 tcaaagtttg ttacgtgatc aatcgcttcc tgacttgtca ctaataccca agccttacct
    16441 tcacaaattc gacccaaatc ttccacaacg gtttgtaact gcaaagtcaa ctcacgatta
    16501 ccgccgatat attgtccgac ctcatcgact agaaaggcaa tatgatggtc tgcttctttg
    16561 gaatcaagat agcttttaac tagatttgca aagtcatcaa tgcttattaa ataatttcct
    16621 gatgcttttt ctaaaaaatt atcaatagcg gcttcactca taaaatcgat tttaagtgcc
    16681 gcttctttga tatcatcttg gatccaatca aactcatggc gctcttcttg ccattttgcc
    16741 ccattaatct cttcaaaagc agctttgaat gcatcaaact tttcttcttt aattaaccga
    16801 cgttcaaaat ctgctagatg tggaatcgtg cttaatcctt gcatttcgtt aaataccttt
    16861 aaaaaaacat tcacgattcc gtttttgtct tttttaccac ctgcatcact ttttgaatca
    16921 atattaaaca gaataacatc tgtatttatt tgtgagcacc gttccatcgt tgccaaagtc
    16981 agtggatctt tgattttccc atcttctttg aagtaatcaa tggctttctt gtcatttatt
    17041 tctcgatttg cgagcaagta agaaataatt tttaagaaat gagatttacc tgaaccaaag
    17101 aacccctgaa tccaaacacc cattttatct gttggtgtat tgatactccg ttggtaggca
    17161 tcaaaaattg ttttaaagtg tttttgtagt tctttcgtaa ccacgtattc ttctaattct
    17221 tgttgaacat tttcatcctt tgcttgaccg accttaataa ctccttgaat atttcggtca
    17281 attggttttg caaaaatatc tttaattagc atttaatctc ctaccttcca tcccaactaa
    17341 attctaaatg ctcgataata attatcatct ttattggtat taagaattgt caaacttaat
    17401 tcatcatatt cccctggata catcatcaca actggtacgc gatcaaatac ctgatgcatt
    17461 ttatttaaaa tagagtgcgc tctaattaat ggatataatt cgccaattcc tgtcaggaac
    17521 acaactgatt tttctggtgt atgggcatcc acatattcaa aaaatacatt ttcttcctca
    17581 ggtaaatcca ttttcaataa acgaccaatg ctttccacta atttttgtaa accacgtcgt
    17641 tcttccatct taatcgcttt ttcagtgaaa ccgcgttttt ctagatactc taacataatc
    17701 acatagagat tataaacttt cacttcataa ttatcaccaa cagatgttcc agatatatca
    17761 ttaatataat ctcttacaat taactcgtct ttcggatcgt agtcaaagat ataatagcct
    17821 acttcattac ctaaaccacg attcaataaa aaatcttctt tttgaattac ttcattcaac
    17881 ttttccaaat gctcttgaat cgataatgac attataaaat tcctcccagt gctagtacaa
    17941 tatctttacg attcttgtca actaaccatt gaatcaatcg tttatctaat aatatcggag
    18001 tcattcgtcc atattctttt tcccctctga ctaatcctgt ttcaattaga ttcgtttgat
    18061 aagcggcaat aagccgttta atcgtataat ctttccattt tgaaatatct tctgaacttc
    18121 tccgcttctc tgtaaaaaaa gaacggtaat caactggtgt taattgcaaa tttcctttac
    18181 gtagattttc agcatatacc tcagtcataa attcattaaa aagatcatca atcataagga
    18241 tggaaatcaa attcactaac ttttgattat ctatatttaa ctgattgaaa agttgcaatc
    18301 cagcttcatc cagttgacta actctccttt tcacgttctg atagatttct ttttgacggt
    18361 tactgctcac tgcaaaaaaa atattttcat gttcattttt ctctttaatt tctatatctg
    18421 tatatccttc tagtcgtaat tgaatatata acttgaactc tgaaaaccaa aagcgtttag
    18481 aaactagacc agcagaatat tttttattca atagattgcc tcctaatcat ctataattca
    18541 ataagtaaac aagtgttttt tgctcatcgt tagcattcat tttttagtat ctactataca
    18601 atacatattt attata
//
LOCUS       DAZKMZ010000048        18457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000048 DAZKMZ010000000
VERSION     DAZKMZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.048"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8M5_001777"
     CDS             99..746
                     /locus_tag="ACG8M5_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0359028.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8M5_001778"
     CDS             1139..3784
                     /locus_tag="ACG8M5_001778"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0359029.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8M5_001779"
     CDS             4094..5407
                     /locus_tag="ACG8M5_001779"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0359030.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8M5_001780"
     CDS             5397..6050
                     /locus_tag="ACG8M5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0359031.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8M5_001781"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8M5_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0359032.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8M5_001782"
     CDS             6947..7147
                     /locus_tag="ACG8M5_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0359033.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8M5_001783"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8M5_001783"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0359034.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8M5_001784"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8M5_001784"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0359035.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8M5_001785"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8M5_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0359036.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8M5_001786"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8M5_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0359037.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8M5_001787"
     CDS             13269..13466
                     /locus_tag="ACG8M5_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359038.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8M5_001788"
     CDS             13619..14104
                     /locus_tag="ACG8M5_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0359039.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8M5_001789"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8M5_001789"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0359040.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8M5_001790"
     CDS             14981..15508
                     /locus_tag="ACG8M5_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359041.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8M5_001791"
     CDS             15599..16336
                     /locus_tag="ACG8M5_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0359042.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8M5_001792"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8M5_001792"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0359043.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..>18457
                     /gene="hflX"
                     /locus_tag="ACG8M5_001793"
     CDS             17259..>18457
                     /gene="hflX"
                     /locus_tag="ACG8M5_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0359044.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKT"
BASE COUNT         5961 a         3195 c         4019 g         5282 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaac
//
LOCUS       DAZKMZ010000049        18328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000049 DAZKMZ010000000
VERSION     DAZKMZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.049"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..506
                     /locus_tag="ACG8M5_001794"
     CDS             24..506
                     /locus_tag="ACG8M5_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359045.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            703..1593
                     /locus_tag="ACG8M5_001795"
     CDS             703..1593
                     /locus_tag="ACG8M5_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0359046.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1918..4389
                     /locus_tag="ACG8M5_001796"
     CDS             1918..4389
                     /locus_tag="ACG8M5_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0359047.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4492..6228)
                     /locus_tag="ACG8M5_001797"
     CDS             complement(4492..6228)
                     /locus_tag="ACG8M5_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0359048.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6225..6929)
                     /locus_tag="ACG8M5_001798"
     CDS             complement(6225..6929)
                     /locus_tag="ACG8M5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0359049.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7060..7563
                     /locus_tag="ACG8M5_001799"
     CDS             7060..7563
                     /locus_tag="ACG8M5_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0359050.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7523..7861
                     /locus_tag="ACG8M5_001800"
     CDS             7523..7861
                     /locus_tag="ACG8M5_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0359051.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7882..9300
                     /gene="ffh"
                     /locus_tag="ACG8M5_001801"
     CDS             7882..9300
                     /gene="ffh"
                     /locus_tag="ACG8M5_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0359052.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9412..9990
                     /locus_tag="ACG8M5_001802"
     CDS             9412..9990
                     /locus_tag="ACG8M5_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0359053.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9994..10515
                     /locus_tag="ACG8M5_001803"
     CDS             9994..10515
                     /locus_tag="ACG8M5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0359054.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10594..11148)
                     /locus_tag="ACG8M5_001804"
     CDS             complement(10594..11148)
                     /locus_tag="ACG8M5_001804"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0359055.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11401..11676
                     /gene="rpsP"
                     /locus_tag="ACG8M5_001805"
     CDS             11401..11676
                     /gene="rpsP"
                     /locus_tag="ACG8M5_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0359056.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11688..11939
                     /locus_tag="ACG8M5_001806"
     CDS             11688..11939
                     /locus_tag="ACG8M5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0359057.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12064..12855
                     /locus_tag="ACG8M5_001807"
     CDS             12064..12855
                     /locus_tag="ACG8M5_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359058.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13025..13546
                     /gene="rimM"
                     /locus_tag="ACG8M5_001808"
     CDS             13025..13546
                     /gene="rimM"
                     /locus_tag="ACG8M5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0359059.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13536..14297
                     /gene="trmD"
                     /locus_tag="ACG8M5_001809"
     CDS             13536..14297
                     /gene="trmD"
                     /locus_tag="ACG8M5_001809"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0359060.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14433..14780
                     /gene="rplS"
                     /locus_tag="ACG8M5_001810"
     CDS             14433..14780
                     /gene="rplS"
                     /locus_tag="ACG8M5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0359061.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14912..16048)
                     /locus_tag="ACG8M5_001811"
     CDS             complement(14912..16048)
                     /locus_tag="ACG8M5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359062.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16164..16730)
                     /locus_tag="ACG8M5_001812"
     CDS             complement(16164..16730)
                     /locus_tag="ACG8M5_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0359063.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16866..17519)
                     /locus_tag="ACG8M5_001813"
     CDS             complement(16866..17519)
                     /locus_tag="ACG8M5_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359064.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17569..17997)
                     /locus_tag="ACG8M5_001814"
     CDS             complement(17569..17997)
                     /locus_tag="ACG8M5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0359065.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18002..>18328)
                     /locus_tag="ACG8M5_001815"
     CDS             complement(18002..>18328)
                     /locus_tag="ACG8M5_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359066.1"
                     /translation="TIAEVERQVGISNGQIRRWDKASPKSENLKKVADYFGVTTDYLL
                     GNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRVKDILIATFWDIVKEDK
                     EKGKKM"
BASE COUNT         5842 a         3349 c         3640 g         5497 t
ORIGIN      
        1 aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat
       61 ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct gttgtacaag
      121 gtgcggagat attaggcctg atccaaggaa gcggatttgt ggtttctagg aattttccag
      181 ctttgactgt tttctattta gtgataagtg taggtctctt attaccaaaa tctctgatca
      241 atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca tctatgtttt
      301 ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga ttgaaggatt
      361 cagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta gtaattgcct
      421 catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa agtgaacgat
      481 ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaagca tatgagctgc
      541 gtaaaatgca tgtttctcat atgctttttt tattattatg taaagaatgc gaaaaacgtt
      601 tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg gatttacagt
      661 cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa agtattgttt
      721 actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg aaatacagat
      781 ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta
      841 ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt acaagaaaat
      901 ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa
      961 gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga cggagttgat
     1021 gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcgccaat cgtcaataaa
     1081 gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac aggaaaaatc
     1141 actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac
     1201 ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac ccctgtgcag
     1261 tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac accaggagca
     1321 atcagctatt tagctttctc atatctcgat gattccacac aagcactaag tatcgacggt
     1381 gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc atatgaacat
     1441 atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta catgatgaca
     1501 gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac aatgatggaa
     1561 gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc cacggtagtc
     1621 gttactatta aggaaaaatc aactatgttt cccacatata catttttcct ttaattgtga
     1681 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattaaac
     1741 accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagctcttt ttttgctata
     1801 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     1861 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     1921 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     1981 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     2041 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     2101 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     2161 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     2221 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     2281 acagcaaaag tcaatgtcat acgggacggg atagaacaaa caatagatat agaagacctt
     2341 gtaatagatg acattgttat tttagagact ggacatcaaa ttccagcaga tgcagaggtc
     2401 gtggaaggaa aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     2461 aaagaaatag gcgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     2521 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     2581 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     2641 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     2701 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     2761 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     2821 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     2881 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt gatcgtctct
     2941 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     3001 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     3061 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     3121 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     3181 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     3241 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     3301 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     3361 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     3421 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     3481 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     3541 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     3601 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     3661 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     3721 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     3781 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     3841 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     3901 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     3961 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     4021 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     4081 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     4141 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     4201 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     4261 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     4321 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     4381 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     4441 cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt
     4501 cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc
     4561 attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg
     4621 tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat
     4681 gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg
     4741 gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat
     4801 aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat
     4861 cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg
     4921 ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat
     4981 ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt
     5041 cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga
     5101 agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc
     5161 taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga
     5221 attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc
     5281 ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc
     5341 ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg
     5401 catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat
     5461 catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg cccgatacat
     5521 cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc attgatcggg
     5581 tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta gtacgggcag
     5641 cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac
     5701 aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa
     5761 aaaaccgctt gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac
     5821 tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca aagcgctacc
     5881 taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa
     5941 tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc
     6001 gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc
     6061 gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc
     6121 cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac
     6181 taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg ttttggttcc
     6241 tgaaacttat agccaaatcc tctaatagtc accaagtact ttggatgttt tggatctcgc
     6301 tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag
     6361 tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc
     6421 atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg
     6481 gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca
     6541 gcttcatctt ttgattgctt acgaggttca atccgtctga atattgcttt catacgggct
     6601 aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca
     6661 atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt
     6721 tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa
     6781 atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca
     6841 ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt
     6901 tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt
     6961 ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa
     7021 taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt
     7081 ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa
     7141 ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt
     7201 cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca
     7261 aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa
     7321 gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct
     7381 tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa aaatggtgcc
     7441 ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg
     7501 ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt
     7561 tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc
     7621 agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata
     7681 tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt
     7741 ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa
     7801 gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata
     7861 aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg
     7921 atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg
     7981 gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact
     8041 aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa
     8101 caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag
     8161 ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt
     8221 aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg
     8281 ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt
     8341 caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc
     8401 cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca
     8461 ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg
     8521 caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta
     8581 gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg
     8641 gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc
     8701 atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc
     8761 cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac
     8821 gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac
     8881 tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg
     8941 atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt
     9001 gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg
     9061 ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg
     9121 aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg
     9181 gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc
     9241 aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa
     9301 gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag
     9361 caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt
     9421 ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct
     9481 gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg
     9541 agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat
     9601 atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga
     9661 attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa
     9721 attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc
     9781 gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa
     9841 ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct
     9901 tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag
     9961 gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct
    10021 ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact
    10081 ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg
    10141 ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag
    10201 aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta
    10261 aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg
    10321 gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg
    10381 tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca
    10441 tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat
    10501 tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt
    10561 acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc
    10621 tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt
    10681 ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac
    10741 attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc
    10801 tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct
    10861 tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt
    10921 aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg
    10981 agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa
    11041 tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta
    11101 agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct
    11161 ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact
    11221 tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa
    11281 tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag
    11341 ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata
    11401 atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc
    11461 gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac
    11521 aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta
    11581 tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg
    11641 caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa
    11701 gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag
    11761 acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg
    11821 gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg
    11881 ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa
    11941 taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga
    12001 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    12061 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    12121 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    12181 ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat
    12241 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    12301 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    12361 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    12421 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    12481 atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    12541 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    12601 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    12661 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    12721 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    12781 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    12841 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    12901 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    12961 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    13021 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    13081 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct
    13141 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    13201 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    13261 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    13321 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    13381 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    13441 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    13501 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    13561 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    13621 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    13681 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    13741 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    13801 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    13861 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    13921 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    13981 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    14041 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    14101 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    14161 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    14221 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    14281 cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag
    14341 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc
    14401 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    14461 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    14521 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    14581 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    14641 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    14701 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    14761 tcaaagaaat ccgtcgttaa tcatcgttat cagacagaga aagaaccctt ggtattcaag
    14821 ggttcttttt ttattatctg tttttttata ttaacgattt ctatttggta catttttgat
    14881 taataaaaag gggcaaaaaa ggggcaacaa gttacagttt ttctatatct tctttcaatt
    14941 tttcttttac tccttttgtc acatgcaaat aaattttttc agtgatatca ctattttcgt
    15001 gaccaacgcg atcctgaatg gcatacagag gagttcctag ttccgctaat tttgaaatat
    15061 gggtatgtct aaaaatatgt gaactaagtt ttttatcaat cttcatgtca gctttatgac
    15121 ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt tcctttcgtt gtttgaaata
    15181 gaaattgacc atttggattt aattctagaa gctcattgta aatagctatc gctttttttg
    15241 gtaaatcaat ttctcgcatt ccggcagctg tttttgtaga atcagatttt ttcatatcag
    15301 ctattgaacg ttctctatac atcatcgtcc catttataac tactgatgca ttattattgg
    15361 taatgtgtac atcatcttta cttaatgcga ttgcttcacc agacctcatc ccagtcaaat
    15421 atagccactg gaaaaggaga gaatatcttt tattgtgtga agttgtgaaa tctactagtc
    15481 tattatattc atcatcttct aaaaatttat ctttaatttt tatcgttttt gactctcttt
    15541 tataatcaat aactacttca tcgataggat ttttttctac ataacctttt ttcatagcat
    15601 atgaaaaaag aagattcaat ttggatttaa ttacactgac gtacttattt gacaagtcat
    15661 ctttatatat catgtcttca aaaatattat tcaaatctat tgtattaata ccagaaacga
    15721 tgtaagtctc aggtatcttc ttctttatag tgtttaatat attattcgta ggataatatg
    15781 tagattcctt aacttgtctt ttgtatataa ctaaccattc ttcaacaagc tcatgaaatg
    15841 ttagatcagg cttatgaagt gttttttcgt ttaattttat atcaattttt ttatttaact
    15901 ctagcaatgc cactttctga gtttctcgag atttattttt ataagtaatg ctcacttttt
    15961 ttctttttct agtttttgga tcaatatatc gctcattata tttatatact ttttttccgt
    16021 ttttatcagt tttagtttcg atccacattg ttttcatctc ctatttgttg ctataatagg
    16081 aatagataag taagccgatt atagcaggtt tatttttcat cacgtccaca aactttggcg
    16141 agggaggggc gtgtttttgt tagttattgt ggtaggttat ctaaagcgta ttgtgcttgc
    16201 tcatttgtaa aaccttcaaa tataagttga tcgtacaatt gcgaatctga cattgatgcc
    16261 caatcatcgt aactttccgc tttagccaaa gcttgttcgt tgtaatcgac atcaacatta
    16321 gccagcgcat aatcgattgc atcacttgga tattcttcaa actctagttg ttcacgcaaa
    16381 ccttgttcag acataccagc ccaacctaga tagctttcag ctttgctcaa tgcatttctg
    16441 tattcacgag gaacactttc ttcttgagta acttcttcta tagtagtttc agtagtttca
    16501 gtttcttctt caacgactgt agattctgct accgattctg cagaagacac ttctgtcacg
    16561 ctttctttag ttgtttcagc gacggtattt ttagcatcat cactgcctcc ttgcgaacct
    16621 atagcaataa acaccacaac aaccgctaac aaccaaaacc atacccgttt ataaaacggt
    16681 ttcttaactt tatacatttt cccatcttga cccataactt tttttgccat ttaaatatcc
    16741 ctcgcttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc cgtgttgcag
    16801 cacgggcttt tttctttata acttttttag agattatagg caaaatagta gggcataaaa
    16861 atgtattatt gaattccgta tttagaaaat cctaattgaa cttcaccgga agtcttttgc
    16921 tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca tgcaacaggc
    16981 gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc ttgctgagcg
    17041 cttgtttgtt ggtcttctgg attctgttga gatgctgctt gttgttcttt ctgatcttga
    17101 ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc cacgccgaaa
    17161 gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg taaagtgcgt
    17221 tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtact ctgactggag
    17281 cttataactg ttgggctagg ttccgccgta gcttggttgg agcttgtaac tgttgaacta
    17341 gaatctgttt tagatgtaga tttgctagta gatgaactgg tttcagttgt ttcttttgtt
    17401 ttacttattt ttgtttcttg gttagaagtg gtatctgttg attcagcttt tttattattt
    17461 gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac ttttttcatt
    17521 ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt atataagaaa
    17581 acgataagcg ctttctggaa gcccgtaaag attctttaat tcctcgattt ttttaggata
    17641 ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc aattagcttc
    17701 gctttcagat ttgcttctag atgttcttgt tgatacataa tagctggata agccacgatg
    17761 aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctcgcag
    17821 ttcatcattt aaaaagatta tcgcacgatc tctaatttct tgaaactgcc ccttgggatt
    17881 ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat aaaaaggatt
    17941 agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca tctccataag
    18001 ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaaa gtcgctatca
    18061 ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt ttccccacca taagacatat
    18121 taacatttga gtctagtagt ttatcaagtt caaccacttc ctcttttgta gcccacttgg
    18181 gaacattatt atttcccaat aaataatcag ttgtgacacc aaaataatca gcaactttct
    18241 ttaagttctc agattttggc gaggctttat cccatcttct tatttgtcca ttagagatgc
    18301 ccacctgtct ttctacttct gctatagt
//
LOCUS       DAZKMZ010000050        17984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000050 DAZKMZ010000000
VERSION     DAZKMZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.050"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..822)
                     /locus_tag="ACG8M5_001816"
     CDS             complement(<1..822)
                     /locus_tag="ACG8M5_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0359067.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELD"
     gene            complement(1126..3291)
                     /locus_tag="ACG8M5_001817"
     CDS             complement(1126..3291)
                     /locus_tag="ACG8M5_001817"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0359068.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3551..5845
                     /locus_tag="ACG8M5_001818"
     CDS             3551..5845
                     /locus_tag="ACG8M5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0359069.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5838..6503
                     /gene="pgmB"
                     /locus_tag="ACG8M5_001819"
     CDS             5838..6503
                     /gene="pgmB"
                     /locus_tag="ACG8M5_001819"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0359070.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6496..7503
                     /locus_tag="ACG8M5_001820"
     CDS             6496..7503
                     /locus_tag="ACG8M5_001820"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0359071.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7525..8544
                     /locus_tag="ACG8M5_001821"
     CDS             7525..8544
                     /locus_tag="ACG8M5_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359072.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8825..10192)
                     /gene="nhaC"
                     /locus_tag="ACG8M5_001822"
     CDS             complement(8825..10192)
                     /gene="nhaC"
                     /locus_tag="ACG8M5_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0359073.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10301..11722)
                     /gene="tyrP"
                     /locus_tag="ACG8M5_001823"
     CDS             complement(10301..11722)
                     /gene="tyrP"
                     /locus_tag="ACG8M5_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0359074.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(11965..13842)
                     /gene="tdc"
                     /locus_tag="ACG8M5_001824"
     CDS             complement(11965..13842)
                     /gene="tdc"
                     /locus_tag="ACG8M5_001824"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0359075.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14133..15389)
                     /gene="tyrS"
                     /locus_tag="ACG8M5_001825"
     CDS             complement(14133..15389)
                     /gene="tyrS"
                     /locus_tag="ACG8M5_001825"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0359076.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(15501..15706)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15969..17270
                     /locus_tag="ACG8M5_001826"
     CDS             15969..17270
                     /locus_tag="ACG8M5_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0359077.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(17339..17938)
                     /locus_tag="ACG8M5_001827"
     CDS             complement(17339..17938)
                     /locus_tag="ACG8M5_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0359078.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         5473 a         3821 c         3257 g         5433 t
ORIGIN      
        1 atctaattcg atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa
       61 aatccgttga tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa
      121 tttttctgta ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt
      181 ttcattcgcg acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata
      241 accttgagag tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc
      301 caaaaagtag ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca
      361 ccaagaaaag tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat
      421 caagatcttg cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg
      481 actttccaat gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat
      541 gtggctcatt tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa
      601 acggtaggca aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat
      661 tagcttcgca ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat
      721 cacttcataa tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc
      781 tggctcaagc cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt
      841 catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt
      901 tctcctgcta actcctcagt aataagctca atggaccagg agctattttt tgtttggctg
      961 tttttatcac ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac
     1021 tgctgcttct cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
     1081 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga
     1141 gccgatcact ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt
     1201 atcgtttgta aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag
     1261 atccatctca gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc
     1321 aaaagctggc tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc
     1381 atctttcgtt tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc
     1441 gatcggcgca acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt
     1501 ttgtgagaat accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga
     1561 aactagttct tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga
     1621 aaccatttct ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt
     1681 caagacatct gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc
     1741 catcgcccct gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact
     1801 tacgcgtaac cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa
     1861 atgaatgatc tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt
     1921 ttcttcactg tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt
     1981 gatcaagaaa tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa
     2041 gttgataaga tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc
     2101 gatattccca aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc
     2161 ttggatcact gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa
     2221 tggttctgtt acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata
     2281 tcttgctttc ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat
     2341 acctgaagaa ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac
     2401 aaactcatat tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc
     2461 caaccataac ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc
     2521 atacgatccg ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa
     2581 tggcaatagt aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc
     2641 ttgtgattct gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac
     2701 cacagcaaga atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg
     2761 ctttccattg aaaaatgaca aagcatccgg caattttcta taattatagt acttattata
     2821 agccattgcc ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg
     2881 tgcttccaat acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg
     2941 ggtaaatgca tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat
     3001 ccgattgatt aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc
     3061 ccaacttcct cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc
     3121 aatattttca attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg
     3181 aattgatttc cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat
     3241 taaagccttt ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag
     3301 ctcctttacg caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat
     3361 gctttatttt ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc
     3421 ttctataatt ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt
     3481 ttgtctattg acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag
     3541 gaggagaaaa atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac
     3601 gacacaatta gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg
     3661 ttatatgggg atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac
     3721 ttatttggcg ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta
     3781 tccagaatac tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat
     3841 caacgatcag ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga
     3901 tatgaaaaat ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg
     3961 catttccttc gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac
     4021 tatagaaatg cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt
     4081 tcaaaacgaa gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat
     4141 agaagaaatc ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt
     4201 tactgttact gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca
     4261 cgaagatact ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg
     4321 gtcagtggaa aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc
     4381 agaaaaagct gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga
     4441 gcaaacagcg gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga
     4501 agaagcacaa caaggtattc gattcaattt gttccaacta ttctctactt actacggcga
     4561 agatgaacga ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac
     4621 ttactgggat acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt
     4681 aactaagaat ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca
     4741 gcaacaagga ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca
     4801 taacgagtgg gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat
     4861 ctacaattat acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt
     4921 tttagcagaa atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa
     4981 atacatgatc cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg
     5041 gtatacaaat acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta
     5101 tcaaaaagaa gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc
     5161 agagaacatg tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt
     5221 cttagataaa gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca
     5281 gcattggtct tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca
     5341 ggggatttat ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt
     5401 ctatgaacca atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc
     5461 agcagaatta ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga
     5521 tttggataat tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag
     5581 ctggttgtcg attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt
     5641 cgcaccattt ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg
     5701 tttattgttc atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc
     5761 tctagaaatt tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct
     5821 aagaaaggag gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg
     5881 atactgctga attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga
     5941 tcgaccacac attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc
     6001 ttttagctta tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga
     6061 aaagaaaaaa cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc
     6121 ccggggtcgt gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt
     6181 ctgcaagtaa aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg
     6241 ctattgccga cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg
     6301 cagcaaaagc tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg
     6361 gtatccaagc catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc
     6421 ttggagagga gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag
     6481 aaaagtggca tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa
     6541 ataaagaaaa tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc
     6601 cgcctgcacc aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct
     6661 tacgatcgtt tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct
     6721 gtagccggaa ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga
     6781 tcacaccata tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt
     6841 ttcaaaactc catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat
     6901 ccaggaccaa tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca
     6961 acctctgcta gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac
     7021 ctttcaggaa atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt
     7081 gtgcttgaat tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg
     7141 ccaatcgact ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga
     7201 ctagatgatg tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc
     7261 gagaagcatt ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt
     7321 tctacgactg gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac
     7381 ggattagcaa tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg
     7441 atcaacctcc atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa
     7501 taacaagaga aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc
     7561 ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca
     7621 acaaagaaga aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc
     7681 cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca
     7741 aaagagcgga tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg
     7801 cggaattatg atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag
     7861 aacgtccagc gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat
     7921 agtcaagata ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga
     7981 cagccttatc atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt
     8041 aaacaagcaa cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac
     8101 acgactcatg aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg
     8161 gctggtcttc ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt
     8221 gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc
     8281 cttcgcacga tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc
     8341 attagcttca acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat
     8401 atcgatatct ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa
     8461 gaattgagca gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc
     8521 gttcctttag aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct
     8581 atggtcagct ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa
     8641 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     8701 ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt
     8761 ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct
     8821 tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag
     8881 gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc
     8941 ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat
     9001 ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga
     9061 atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga
     9121 tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt
     9181 cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa
     9241 gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat
     9301 ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc
     9361 cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta
     9421 gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa
     9481 tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac
     9541 tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga
     9601 cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc
     9661 caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg
     9721 caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc
     9781 ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga
     9841 caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc
     9901 cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa
     9961 tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat
    10021 ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg
    10081 caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca
    10141 acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc
    10201 tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta
    10261 ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt
    10321 tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga
    10381 acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg
    10441 tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc
    10501 cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt
    10561 taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg
    10621 taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg
    10681 aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat
    10741 ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg
    10801 gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga
    10861 tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga
    10921 tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga
    10981 cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg
    11041 cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga
    11101 taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg
    11161 aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta
    11221 aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga
    11281 cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac
    11341 aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca
    11401 atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc
    11461 atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc
    11521 cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa
    11581 acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa
    11641 tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt
    11701 tacttacttc agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg
    11761 tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct
    11821 ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag
    11881 cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc
    11941 tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta
    12001 gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg
    12061 ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc
    12121 acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc
    12181 atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc
    12241 tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc
    12301 atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg
    12361 atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa
    12421 acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg
    12481 taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa
    12541 gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa
    12601 gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg
    12661 aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata
    12721 tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta
    12781 atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc
    12841 acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc
    12901 taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga
    12961 tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat
    13021 tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg
    13081 gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg
    13141 aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat
    13201 ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt
    13261 cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag
    13321 cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc
    13381 agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac
    13441 ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca
    13501 tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc
    13561 ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc
    13621 tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc
    13681 tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc
    13741 caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa
    13801 tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa
    13861 aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt
    13921 tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt
    13981 tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat
    14041 taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat
    14101 ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa
    14161 tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg
    14221 acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct
    14281 tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt
    14341 tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc
    14401 gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc
    14461 aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca
    14521 cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag
    14581 aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg
    14641 tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt
    14701 ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct
    14761 tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct
    14821 gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat
    14881 tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg
    14941 atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca
    15001 agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta
    15061 ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc
    15121 gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg
    15181 ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga
    15241 atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg
    15301 ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg
    15361 cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa
    15421 agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa
    15481 aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt
    15541 ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag
    15601 actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct
    15661 gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg
    15721 tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc
    15781 tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa
    15841 aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct
    15901 cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga
    15961 cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg
    16021 ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca
    16081 ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag
    16141 ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg
    16201 aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg
    16261 agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca
    16321 gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt
    16381 atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg
    16441 ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg
    16501 ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt
    16561 atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa
    16621 tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc
    16681 tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct
    16741 tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag
    16801 acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga
    16861 aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac
    16921 tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa
    16981 aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag
    17041 cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg
    17101 atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca
    17161 atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat
    17221 tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca
    17281 tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt
    17341 atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa
    17401 gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga
    17461 tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa
    17521 gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt
    17581 ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat
    17641 tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt
    17701 ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat
    17761 aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt
    17821 tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa
    17881 aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt
    17941 gtagagcctc ccgtaatttt aaacttttat aatatatata gttc
//
LOCUS       DAZKMZ010000051        16266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000051 DAZKMZ010000000
VERSION     DAZKMZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.051"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..431
                     /gene="scm"
                     /locus_tag="ACG8M5_001828"
     CDS             <1..431
                     /gene="scm"
                     /locus_tag="ACG8M5_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0359079.1"
                     /translation="TSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTD
                     TSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNK
                     SKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(586..1572)
                     /locus_tag="ACG8M5_001829"
     CDS             complement(586..1572)
                     /locus_tag="ACG8M5_001829"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0359080.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1595..2161)
                     /locus_tag="ACG8M5_001830"
     CDS             complement(1595..2161)
                     /locus_tag="ACG8M5_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0359081.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2175..2591)
                     /locus_tag="ACG8M5_001831"
     CDS             complement(2175..2591)
                     /locus_tag="ACG8M5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0359082.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3048..4982)
                     /locus_tag="ACG8M5_001832"
     CDS             complement(3048..4982)
                     /locus_tag="ACG8M5_001832"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0359083.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5284..6219
                     /gene="pplA"
                     /locus_tag="ACG8M5_001833"
     CDS             5284..6219
                     /gene="pplA"
                     /locus_tag="ACG8M5_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0359084.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6309..7412
                     /locus_tag="ACG8M5_001834"
     CDS             6309..7412
                     /locus_tag="ACG8M5_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0359085.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7425..8378
                     /gene="menA"
                     /locus_tag="ACG8M5_001835"
     CDS             7425..8378
                     /gene="menA"
                     /locus_tag="ACG8M5_001835"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0359086.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9192..10538)
                     /gene="gor"
                     /locus_tag="ACG8M5_001836"
     CDS             complement(9192..10538)
                     /gene="gor"
                     /locus_tag="ACG8M5_001836"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0359087.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10672..11925)
                     /locus_tag="ACG8M5_001837"
     CDS             complement(10672..11925)
                     /locus_tag="ACG8M5_001837"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0359088.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11931..12854)
                     /locus_tag="ACG8M5_001838"
     CDS             complement(11931..12854)
                     /locus_tag="ACG8M5_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0359089.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13075..15567)
                     /locus_tag="ACG8M5_001839"
     CDS             complement(13075..15567)
                     /locus_tag="ACG8M5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0359090.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15574..16041)
                     /locus_tag="ACG8M5_001840"
     CDS             complement(15574..16041)
                     /locus_tag="ACG8M5_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0359091.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16177..16250)
                     /locus_tag="ACG8M5_001841"
     tRNA            complement(16177..16250)
                     /locus_tag="ACG8M5_001841"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16214..16216),aa:Arg,seq:acg)
BASE COUNT         4778 a         3404 c         2895 g         5189 t
ORIGIN      
        1 caacaagtga tagcacggac acgtcagctt caagtgacag tacagatact acctctacca
       61 caagtgatag ctcagattca tctaccacta caagtgatag cacggacacg tcagcttcaa
      121 gtgacagtac agatacatcc tctaccacaa gtgatagctc agattcatca acttcaagcg
      181 atagtacaga cacgtcgtct accacaagcg atacacgtca tgcaagtgtc tccttctcaa
      241 aaacaagttc ggataacagg acgaacttta acttaactaa taaaacaagc aatgacaatg
      301 gaaacaaatc aaaaagagca cttcctaaaa ctggaagtca atcgaataat tggataacac
      361 tcgcaggcgt tatattactt gtaatcggtt taagaatcac ttttatgagt tataagaaat
      421 cgaaaaggta aaaaaatatt caagataaaa aagagcaacg tcgctaacgg acgttgctct
      481 ttatttttgc tttttatcat aaatacaaga actacaattc aatttgtcaa aaaacacgta
      541 gatttccttc tgagaaattt atttacgtat cgaacctttc cctattcagt ctttgcgatc
      601 caaaatcagt tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg tggcaggtaa
      661 tttctttatt tccttcaaag ccttttctgt gtaagaggcc gctaacttcc tagcttcttc
      721 cacgcctcct gtatgttcaa cgattttgcg tacggcgtcg acatcttctt ctgtcattgc
      781 cgctttcttc tctaaataag gtagtagctt tggtttgttt ttttgcaaag agtagatcaa
      841 cggtaaagaa tagacccctt gacgcatgtc ttctagtaca ggtttgccga tttcctggct
      901 ctcttttgta taatctaaaa tatcatcgat aatctgaaag gccatcccga tagaaagtcc
      961 gatatctcct gctttttttg cgaagcgctc gcttgttcca ctttcaaaag cgccaacaga
     1021 acaacttaat tggaacagtt cagctgtctt acctgaaata ttttccaaat attcttttac
     1081 agaaagttca taattatagc gatcatccat ctgccctaat tcgccattca atactttttc
     1141 catactgcga gaattcaatt ggagactttt caaggaagtg gagtaatcag acaatagttt
     1201 aaaacagcag acaaataaat agtctcctgc atagacagaa acgacattac caaactgact
     1261 acggatatta ggctgaccac gtcgcagatc cgcttcatca acgatatcat catggatcag
     1321 tgtggccgta tggagcattt cgatagaagc tgccaaagcg atggctttgt ctcgatcttg
     1381 ctctggtcca aattgtgaga acaatagctg ataggcagga cgcaacagct ttccgccggc
     1441 atggatcgtt tctttgattg cttgttcgac aggtttgttt ttcaggcgga cagtttcttc
     1501 catccgtttt agtgtttctg tcaattcttt acttaaagca ggatagtttt cccacattgg
     1561 atgtagcttc ataaaaaaac tccttgtgtg acgattattt tttggtaaac attgggctcc
     1621 atttttgttt ggttccctgc tcataatctt tttggaatcg ttgaagtgtc tgcacatgtc
     1681 gtaaaagata attcgctgcg atcccgattg caatccctga taaaatccct aaaactgaaa
     1741 ggactggcaa gtacaacatg actgtccatg attgtgcaat gaaagaagcc atcaaaagct
     1801 gtccgacatt atgcatgaaa ccgccggccg cactaattcc gatgatactg acacgttttg
     1861 gtcctaattg cttgaccaat aacattccaa aataactaag caaagcacca ctcatactat
     1921 aaaggaatgt ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt agccaaagta
     1981 gtaagaagct gtctcgtttt ttcatggtaa aaatggcaac gatcgtgatt agattggcta
     2041 agcctagttt tgcaccaggc gcaaaagaaa aaggataagg gatcatattt tccaataaac
     2101 caatgatcac tccttgtgca acaagtagcg aaatataaat cattttttgt aatttactca
     2161 tggtgtgtga aggtctaata aatcagactg tcttcgtcac ctccatctgt tgaacggact
     2221 tcgatcacta atttatgtgg caaacagacg atggtttcgc catttttcgt tgcccagcct
     2281 cgacgtacgc agatctggtc atcgcaatct gcttctttta tccgtactcg atcgccatct
     2341 atctcgatca agttataatc tccgtgttca tctttgtaag tatatgtgtt gctctcttct
     2401 ccagccatca aagggaatgt tttgatttct gtgccatcca cacgcagtac cgcttctttt
     2461 tctgctgttg cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa agaaagaaca
     2521 gtcaaaagca aaataataaa aacatcccaa ggtttgagca gacttttttc agcaaattct
     2581 ttaaggctca tttttgttct cctttttcca gattaactca cccaatttta gcataaaaac
     2641 aagaggttgt gacacctgtg tcttgctttg agtaataaga acgaagaata gaaaacagct
     2701 ttccatgttt tctaattctt tgggcttatt accgagaagc agcaggtgga acaccgttta
     2761 taacaagagg ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat ttacgaaaat
     2821 tacttttcaa gtttttgtga aatttcggct tatttttcga agaagctact tctggaacac
     2881 cgtttataac aagaggttgt aacagaggtt gtgacacctg tgtcttgctc atggaggctt
     2941 atctcgggca tcttattcct ttaattttac ataaaaagga ggctgtgacg gtttgtctca
     3001 gcccctttct attgaaatct tgcattcaaa taacgttctt tatctattta tttcttgccg
     3061 tactgggaac ttggtctacc gtaccaccaa ttacctagtg tacgttgtag ccatggaatt
     3121 acccagcgat ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa agcaacaaaa
     3181 atgaaccaaa tgtttaccca gtaaaacatg cctgataagc agaacgtgat caccaataca
     3241 atcgttgtcg cactgcatag ccatgtgaat aatccagcaa tcaatgctaa tgccaaggca
     3301 atttcaaaga ataccatgaa tttttggaag aacattgcta catcattgtt tggcatgatc
     3361 acttgcatga tctttgtgaa ccagtctggt gacttctcaa tcacagccat tggttcttca
     3421 ccgtatgcat aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc ggcacttgcc
     3481 cctgctccgc tggcagctgt ggttgcatca ccagcagcag aagcaccact ggcaacatct
     3541 gttacattgt attcccatgt ccaagggaac acactgtttc cagtgaacca tgatccttca
     3601 ccaaaccaag tacttggttt gaacacttca ccattcccaa tgattttttt ggaagcttca
     3661 acgaaccaaa ctaaaccata gaaaatacga agtggcacac tccataagac attgccgtaa
     3721 cgagaagaat gtcctcgagt cacgttgcga tcatctttga tatggaaaaa ctcatgcatt
     3781 aaatattgga acatataata accagaacga atatcaaaga agtatttcaa attcacgata
     3841 tgtttcatta cgatagctaa aaatccgctt aaatggatct tatcaaatag gttagctacc
     3901 ccccatttgg caccaataga aaccataaat ccttgatagt ttcctttaaa tttatgcttt
     3961 tctccgcctt tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt ttgttcagca
     4021 gcttgaacga tctgcggtgt cggtgttcct gggaattctt cataaaagac taaatcacca
     4081 atgatataga tgtttttatc ttcgtagcct tttgcctgca tatattcatt ggcgatcaac
     4141 cgctgtccgc gtgctgattc taatccaaag tcagcagcgt cagaagttcc tttgacacca
     4201 gctgtccaaa tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt gatatgatca
     4261 gcagccactt ctacgattgg tgcattcaat aataattcca cgttgtgctt acgcaaatag
     4321 cgttcagctt ttgcagcatc atttcgatcc aacatattca agatcgtcgg cattgcctct
     4381 acgaccatca gtgtgatttc gtttggatct aatttataat ctttggctag gcgatttttc
     4441 cagtcgatca gttccccaat catttcaata ccggtaaatc cagaaccaca gacaacgaaa
     4501 gtcagcatgg ctttacgcag ttcatcatct ggttccaatg cagcttttgc tactgtcttc
     4561 tcaatatgtt cccgaatcct tacagaatct tcaaatgacc aaagggtgaa tccgtgtgct
     4621 ttgacaccag gtgtaccaaa gtcattcggc tctccgccca ttccaaggat cagctgatca
     4681 aaaggatagg aacctaattt ggtcttgacg atttttcgtt ctttatcgat accgattaca
     4741 gtgtctgtga caagttgtac attcttttga cgagcaaaca aatgctgcag atcgtattgg
     4801 attgcttctg gttctacacg tccgccagct acttcatgca actcggtcat catagtatgg
     4861 tacgaatgac gatcgatcag agtaattgct acatcttctt ttttcagttt ctttgccaag
     4921 tattttgtag ctgaaacacc ggcatatcct gctcctacaa cgacaatatt ttgctttgcc
     4981 attgatcatc cgctccttat agactatcta tataatgaat atatgataat tcttttagtt
     5041 tgatacttat tacacaatct ccatatatta tatagatgtt ctgttctttt gtctaactcc
     5101 ttttggattt ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg ctgaaagaaa
     5161 acgtttgaaa tattacatgg gttatttatc aaaaatagtt gacttatagg ctaaaaagat
     5221 tagaatgtat aatgaatcac aaagaaaaat tgtggactag aaatggaatg gtggggtatt
     5281 aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat tagcattttc agctctagtg
     5341 ttaggcgctt gcggcgcaga tgacaacgga agctcaaact cttcttctga atcatctaca
     5401 gcacaatctt cgactgcaaa aagcacaaca gaatcatctg caaaagttgt agctggcgga
     5461 gatttgcaag acggcacata taaattagaa gaaaaaaatt attcaaatgg ttaccgtgca
     5521 gtctttgaaa tggttgtaaa agacggcaaa atcactgaat caaaatatga taacgtcaat
     5581 gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca aaaacatgga agcaaaatct
     5641 ggcacaaacc cagaaaaata cattcctgag ttgaatgacc aattcttaaa agcacaaagc
     5701 gcaagcggtg tggaagtcgt aactggagct actcactcat ctgaaagctt ccaaaactac
     5761 gcacaacaat tgatccaagc tgctcaagct ggtaacacag atacaatcga aatcgacaat
     5821 ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa agaactactc taacggctac
     5881 cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa ctttgattat
     5941 atcgacaaag acggtaaatc aaaacaagat gatacagaat ataatgaaaa tatgaaagca
     6001 aaaagcggta cagaaccaaa aacttacatc cctactctga acgacgaatt cgtcaaagca
     6061 atgggcgaag aagatggctc acctgctaat gtagaagttg ttacaggtgc tacacacagc
     6121 tcacattcat tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa aggcgacact
     6181 caaacaatcg aagtagataa tatcgtaaca gaaaaataat cacataatca tactaataaa
     6241 agttggaggg tgggacgata gccagattag gcagtcgtct cacctttcaa aatgaagaga
     6301 ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac actgggcatc tcttttatgg
     6361 tgctaactgg ctgcagcaac tcagcaaata aaacaaccac tgaaagttca gcagctacca
     6421 agatattgaa agatccatat tctgatgaac agttcttgtt agggacttac gttcgtatcc
     6481 gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga gtaaaagaac
     6541 ttggtgataa aatcacgatc aatcaaaaag gttctgagat cgatgaagtg aatgagcaag
     6601 ctgggatcaa accagtaaaa gtttctgatg acgtctacac gttagtcaag cgggcctacg
     6661 aatatagcca agattctcaa ggcggattcg atatggcgat tggtgcaatc acacaattat
     6721 ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga agaaatcgat caggcattga
     6781 aattagtgga ttatcataag atcgaactaa atgacaaaga aaaaactgtt tatctaaaag
     6841 aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg gacgaagtag
     6901 tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc ggtaatgtct
     6961 atgtcttagg ccacagccca agaggcgaaa accaagactg gacagtcggc atccaagatc
     7021 cgaacatggc tcgcggatct gttttaggtt ctattaaaga acggaacaaa acattagtta
     7081 cctctggaat ttacgagcgt tatttagaag tagatggcaa aaaataccat catttgtttg
     7141 atccaaaaac gggttatccg ttcgacaatg atatcgcaag tgtcacgatc ataacagata
     7201 aatcaattga tggtgacggg ttgtctacag cagtcttttc aatgggtgtg aaaaaaggac
     7261 tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg acaaaagacg
     7321 ataaagtata tgtgaccgat ccaatcaaag acaccttcaa attatcggag gactcccatt
     7381 acacgatggg tgaccgttct gaactaaaat aaggagtacg aaccatgaca ctaaaaatct
     7441 ttttagaagt agttgaaata cgaacaaagg tggctagtgt atttcctttt gcggtagggg
     7501 tattattctc tatcgctttt tttcatgaga tacattggct aaacactttt ttatttttta
     7561 ttggaatgct agtttttgat ttagcgacta ccgctatcaa taattacatg gattatgaaa
     7621 aggctcattc tgaagtatat aagtatgaag aaaatgtcat tggccgcaga aatctatctc
     7681 ctgtattgat tcgaaacatg attttagcta tgattgcatt tgtttttgtt atcggcgtta
     7741 ttctaacgat caatacgggt tggcttttac ttttgatggg atttatctgt tgttttattg
     7801 gagtattcta tacattcgga ccgattcctt tatctcgaat gcctttaggg gaaattttca
     7861 gtggggtgac tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat acattcggtt
     7921 cagatgtcta tgatctgaca gtcaactttt catctggaca atttgctttg gtaggaaatc
     7981 tttgggctat agcagcaatc gtattagctt ccttaccctt ggtgtttacg attgccaata
     8041 tcatgttggc taataatttg cgggatcttg accgtgatat tgaaaatcat cgctatacac
     8101 tagtttttta tatcggacgt cctgcgggaa cagtcttatt ccagctattg atgtatgctt
     8161 gctatttcgt gattttaatg gggttgttga gcggtatcta tgaatggccg atcttggtca
     8221 ccttcttgac cttgccaatt gtgaagaagc atttggatca attcaagcaa acgttacccc
     8281 agcctatcag tttcagctat tccatcaaaa atatggtctt attcaatgcc agttatgtat
     8341 tggggctatt actaacaatc attatgcaag ccatttaaca gcatgaagag gttgtgaaaa
     8401 aactgccctg catcaagtaa taagaacagc caaataaaaa tagctcctta tatttttcgt
     8461 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttatcag gaggttgtga
     8521 aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt
     8581 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgta
     8641 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
     8701 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
     8761 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
     8821 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
     8881 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
     8941 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
     9001 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
     9061 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
     9121 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
     9181 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
     9241 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
     9301 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
     9361 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
     9421 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
     9481 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
     9541 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
     9601 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     9661 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     9721 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     9781 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     9841 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     9901 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     9961 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
    10021 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
    10081 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
    10141 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
    10201 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
    10261 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
    10321 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
    10381 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
    10441 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
    10501 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
    10561 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
    10621 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
    10681 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
    10741 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
    10801 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
    10861 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
    10921 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
    10981 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
    11041 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
    11101 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
    11161 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
    11221 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
    11281 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
    11341 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
    11401 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
    11461 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
    11521 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
    11581 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
    11641 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
    11701 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
    11761 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
    11821 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
    11881 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
    11941 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
    12001 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
    12061 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
    12121 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
    12181 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
    12241 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
    12301 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
    12361 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
    12421 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
    12481 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
    12541 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
    12601 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
    12661 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
    12721 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
    12781 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
    12841 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
    12901 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
    12961 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
    13021 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
    13081 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
    13141 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
    13201 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
    13261 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
    13321 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
    13381 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
    13441 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
    13501 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
    13561 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
    13621 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
    13681 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
    13741 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
    13801 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
    13861 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
    13921 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
    13981 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
    14041 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
    14101 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
    14161 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
    14221 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
    14281 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
    14341 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
    14401 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
    14461 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
    14521 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
    14581 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
    14641 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
    14701 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
    14761 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
    14821 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
    14881 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
    14941 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
    15001 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
    15061 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
    15121 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
    15181 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
    15241 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
    15301 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
    15361 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
    15421 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
    15481 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
    15541 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
    15601 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
    15661 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
    15721 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
    15781 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
    15841 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
    15901 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
    15961 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
    16021 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
    16081 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
    16141 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
    16201 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
    16261 taaaaa
//
LOCUS       DAZKMZ010000052        16062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000052 DAZKMZ010000000
VERSION     DAZKMZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.052"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..685)
                     /locus_tag="ACG8M5_001842"
     CDS             complement(38..685)
                     /locus_tag="ACG8M5_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0359092.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(693..1379)
                     /locus_tag="ACG8M5_001843"
     CDS             complement(693..1379)
                     /locus_tag="ACG8M5_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359093.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1385..2458)
                     /locus_tag="ACG8M5_001844"
     CDS             complement(1385..2458)
                     /locus_tag="ACG8M5_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359094.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2680..3354
                     /locus_tag="ACG8M5_001845"
     CDS             2680..3354
                     /locus_tag="ACG8M5_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0359095.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3359..4699
                     /locus_tag="ACG8M5_001846"
     CDS             3359..4699
                     /locus_tag="ACG8M5_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0359096.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4848..5834)
                     /locus_tag="ACG8M5_001847"
     CDS             complement(4848..5834)
                     /locus_tag="ACG8M5_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0359097.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6558..7589
                     /locus_tag="ACG8M5_001848"
     CDS             6558..7589
                     /locus_tag="ACG8M5_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359098.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7698..9230
                     /locus_tag="ACG8M5_001849"
     CDS             7698..9230
                     /locus_tag="ACG8M5_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0359099.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9369..10181
                     /locus_tag="ACG8M5_001850"
     CDS             9369..10181
                     /locus_tag="ACG8M5_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0359100.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10171..10656
                     /locus_tag="ACG8M5_001851"
     CDS             10171..10656
                     /locus_tag="ACG8M5_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0359101.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            10679..11224
                     /locus_tag="ACG8M5_001852"
     CDS             10679..11224
                     /locus_tag="ACG8M5_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0359102.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11297..12940)
                     /locus_tag="ACG8M5_001853"
     CDS             complement(11297..12940)
                     /locus_tag="ACG8M5_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0359103.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13069..15297)
                     /locus_tag="ACG8M5_001854"
     CDS             complement(13069..15297)
                     /locus_tag="ACG8M5_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0359104.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(15330..15809)
                     /locus_tag="ACG8M5_001855"
     CDS             complement(15330..15809)
                     /locus_tag="ACG8M5_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0359105.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4740 a         3062 c         2907 g         5353 t
ORIGIN      
        1 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
       61 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
      121 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
      181 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
      241 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
      301 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
      361 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
      421 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
      481 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
      541 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
      601 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
      661 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
      721 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
      781 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
      841 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
      901 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
      961 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
     1021 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
     1081 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
     1141 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
     1201 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
     1261 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
     1321 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
     1381 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
     1441 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
     1501 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
     1561 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
     1621 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
     1681 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
     1741 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
     1801 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
     1861 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
     1921 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
     1981 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
     2041 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
     2101 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
     2161 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
     2221 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
     2281 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
     2341 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
     2401 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
     2461 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
     2521 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
     2581 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
     2641 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
     2701 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
     2761 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
     2821 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
     2881 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
     2941 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
     3001 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
     3061 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
     3121 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
     3181 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
     3241 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
     3301 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
     3361 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
     3421 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
     3481 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
     3541 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
     3601 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
     3661 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
     3721 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
     3781 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
     3841 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
     3901 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
     3961 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
     4021 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
     4081 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
     4141 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
     4201 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
     4261 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
     4321 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
     4381 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
     4441 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
     4501 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
     4561 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
     4621 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
     4681 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
     4741 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
     4801 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
     4861 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
     4921 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
     4981 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
     5041 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
     5101 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
     5161 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
     5221 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
     5281 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
     5341 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
     5401 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
     5461 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
     5521 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
     5581 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
     5641 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
     5701 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
     5761 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
     5821 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
     5881 tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt
     5941 aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa
     6001 tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga
     6061 tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct
     6121 caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat
     6181 ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa
     6241 atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag
     6301 cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta
     6361 aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa
     6421 atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga
     6481 tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact
     6541 attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc
     6601 aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc
     6661 tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga
     6721 gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt
     6781 atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc
     6841 aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat
     6901 taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt
     6961 tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt
     7021 tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta
     7081 gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg
     7141 gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga
     7201 ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga
     7261 tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg
     7321 tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca
     7381 aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg
     7441 cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct
     7501 catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag
     7561 cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa
     7621 ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa
     7681 aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga
     7741 attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt
     7801 cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga
     7861 aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata
     7921 agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc
     7981 ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag
     8041 aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg
     8101 gaacaaaatc aaaacagatt cctattcttt atttaatgga acaacctgct ttgtctgttt
     8161 ctcaatcatt tttagacaaa aatgaaattt cttatatgac ccatacttct ggtacaactg
     8221 gtatccctaa acttatttgt cattctgctc attctatggg atggcggacg aaatggcaaa
     8281 aaacagtttt cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc tctccagtgc
     8341 attccagatt caatattggt atctcttctt tgatgtccat gggatttcct atgatgcctt
     8401 tagcaaacgc acaaagttca aaagttgttc atatgttgga agctcaccgg ccaattgcac
     8461 tagagaccca tccgaataac tttgttcagt ggcgttttac tgctaaggag catccagaag
     8521 ctttttcggg tatccgatac tatcactcca cttttgatgc tataaacaac cagacaatga
     8581 agacattttt gcgaacgtcg atggcacagg atgcggtatt tcttcaagta tatggccaaa
     8641 gtgaatgcgg accaatgatt cttaaggctc atacactaga gagtttgaaa acaagtgatg
     8701 caagagatat gggcgttgga ctggaagaca tgacaagcgc tcggattaca gattcagttg
     8761 ggaatgtcct acctgaaaac acagatggtc atatacaatt actgtctaaa ggacgggctt
     8821 tgacttatta caaggaagat gcacggttcc aggaaaatgt ttatggcgat tggtgggata
     8881 gcggagatta cggttttatg gataagcatg gccacctatt tttgaaagat cggcaagtgg
     8941 atttgatcga aacgatcaat agtacattag ctattgaaga ctttttatta gattcgttag
     9001 attttttggc agaagtagtg attgtaaggg ataaaaatca ttctccacaa ccgattattg
     9061 ctttagctcc tgataaagaa atggattgga atcgatggtg ggagcaagtc catgaacttc
     9121 ctcgcttgaa cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca gctacaatga
     9181 aagtgcaacg gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa ctatttaagg
     9241 aaagaaagat ccattttggt ctttcttttc tttttgtttt tttaaattgg atgagataaa
     9301 aatatgccaa ttggctgttt ttcttttttg aaaatttcgt tatactctta ttcatggagg
     9361 aaaatgttat gaaaagaatc ttgacaattg gcggttctga tccatttgct ggtggaggaa
     9421 tccaaacaga tttaaaaact tttgagaatt ttggtctatt cggactaagc gccttgactt
     9481 ctataggggc actagatatt aatgagcgat ttatgctgga atctatatct ttggaattgc
     9541 ttaaacgaca gttagtttcg attgataaaa tgacttcatt agatggtatc aaaattgggt
     9601 tattgaattc aagtgaagca atcatggttg ttcgagactt cataaaaaca aaacaaaata
     9661 tcccaatcat tttagaccct gttttggcat tcaaagaaac tgaagctgtg atcaatcaat
     9721 tgtatatttc gcagctcatc gaagaacttt ttccacttgt tacgatgatt acgcctaatt
     9781 tggcagaagc tgctttactt tctggtcaag ctcttccctc ttcgttaaca gaaatgatcc
     9841 atctcgctaa agaactcact gcttcaggaa caaaaaacat tgtgatcaaa ggcggcacaa
     9901 ggatggctgg agatgaagct gtggatcttc tctattcaat tgatcattat gaggtatttt
     9961 ctcataaaaa aatcgacact acaacagtaa acggggccgg ttgtgccttt tcttctgcaa
    10021 taacagcgaa tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa agtaaaaaat
    10081 ttgttgccca ttgtatttct aatggcgtgc tgatgaacga tcataccgga agtgtctggt
    10141 ctggcggtat tcaaaaagga ggaatgagaa atgaaaacta agcaattaac gatctatgct
    10201 atgctgattg cgttgacagt tgccctttca ttaacaattt tgatccctgt accggctacg
    10261 aatggatttg tgacattatg cgaggccggg atctataccg ctgcttttct ttatggtcca
    10321 acgggtggat tagttgttgg tgcttccagt ggtctattga tcgatttgat ttcaggatat
    10381 ccgcagtggg cagttttttc attagtgatc cacgggttac aaggatatgt tgctggttct
    10441 ttcagcaaat cttctactcg tatgtgggga ttagggcttt tattaggaag cttagttatg
    10501 atcatcggct atggtatcgc cggttggatt ttatatgatt ggccggctgg agtggcttct
    10561 ataccaggaa acatcttcca aaatctgatt ggaatgggac ttgcctttcc tataactgca
    10621 agtcttcaac gtattcaagc acggaagcaa tattaattga aaaagaaggt tttagaaaat
    10681 gaagttgtca gaaaaagaat taaaagacca attgactgaa atagttaatg acatattggc
    10741 tgaggcacac ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa gcgaagtcgt
    10801 tggaggacat attggaaaaa attccagtgc ggaagttggt caatggatca tccgtacgtt
    10861 aaaagagttg ttggatccta aagaaattgc gctagctgtc caaggttgtg aacatttgaa
    10921 ccgagcatta gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa ttgtctctgt
    10981 tgttcctgca ctacatgctg gaggtgcttg ttctgttgct gcttttgacc agttcaatga
    11041 tcctgtcgaa gtggaacacg tagttggaca agccggtatc gatatcggtg acacatcaat
    11101 cggtatgcat atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa cccttggaca
    11161 agctcacgtg acagcccttc gctctcgtcc taaatatatc ggtggtccta gagcgaatta
    11221 ttgaaatgag taattgacag agcaatctga aaaacttcct cgcaaagtcc aactaatgaa
    11281 ctttaacgag gaagttttac ttgtttgttt tatataaccg agcttcataa ggtgctagta
    11341 tgcctttagc ttcaaattct ccgctagagg aatttgacaa gagtaatgtc caggactgat
    11401 ctgcttcttt tggaagttca tagtcagcaa gtgattctgt taagtttacc ataataagat
    11461 attgttcatc ttctaaatat ctttcataga cataaagttg tggatgttca tgaagatatt
    11521 ctttataaga accgtatatc agacctttat tggtttgtct aatgcggatc atgttcttat
    11581 agaattgcag gaccgactct ggttcatatt cttgttgttc tacattgatc gttttggtat
    11641 ttggattagt gatcagccaa ggttcatgtg tagaaaaacc agcatattgt tccgaagtcc
    11701 attgcatggg tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc aatgcttctt
    11761 ctcttgtctt accttccgca agcagccttt tataaagtct tttcgaatct actgcatcaa
    11821 cttgatcgat cgattcaaag ggcatattcg tcattccgat ttcctgtcct tgataaataa
    11881 atggcgtacc ttgcaaaaag aaatacatca tagcgattgc ttttgcagac atagcccaaa
    11941 aatcgggacg agtatctcca aacactgaaa cggcacgagg aacatcatgg ttttccatat
    12001 ataaagcgtt ccatccgata ccatccaatg aagtttgcca agcgcttaat gcatgtttca
    12061 atttaaggac atctaattgc ccttcttttt cttgctgcca gatatggata tgatcaaatt
    12121 cgaatatcat atcgaaataa ccgtctttcc ctacccattg tggtccttct tctgcagtga
    12181 caccacttgc ttctccgaca gtcatgatat cgtactcttc aaaaacatgt ttcaaatcgg
    12241 tcaaatgttc ttcgattcct gagacatttt gaaagggtga gaaagggttc tctgtggctt
    12301 ttactgaata ttcatctttt tttacatgag aaatcgcatc taaacgaaat ccatctatcc
    12361 ctttatccaa ccaccatcgg atcatattga atagttcttc tttcaatttc tcgctttccc
    12421 aatttaagtc tggttgttct tttgcaaaaa catgcagata atactgtcca gtttcatgag
    12481 aatactccca agcagaaccg ccgaaaatcg atgtccaatt attcggttct ttgccatcta
    12541 ccccgtcgac ccaaatgtaa taatcgcgat acgggctttc tttagatttt ttcgattctt
    12601 caaaccattg atgctgatcc gaactatggt tgatgacaag atccataatc actttgatcc
    12661 ctaattcatg tgcttctttt agcaataaat caaaatcttc catcgttccg aatctctctt
    12721 ctatatcttg atagtcactt acgtcataac cattatcgac aaatggagat tgatagatcg
    12781 gattgatcca gataaagccg ataccaagat ctttcaaata attaagtttt tcacggatcc
    12841 cattgatgtc tccttgacca tcgccattgc tatctttgaa actagcagga tagatttgat
    12901 agcctactgc ttctttccac catttttgtt cttgtatcat atcgtttcac tcctttaacg
    12961 gtccctcttg attataaccg attctatttt agaagtatcc ttgttaccgg taactagaaa
    13021 cgaaaagctc aacgaatctg ctaagtgcgt tcgttgagct ttattttatc atagatagcg
    13081 ggcaatcagt tgaatcattt gcttgggaaa ttcattcaag tcagtaatat ctacaaaacg
    13141 actttcaccg tagatcgact tgatttcttc tttgtcttgg ccaatagctg cggagacaaa
    13201 taggattcct tgacgttcat attctttcaa aacagactgg atatcttctt ttgctttttt
    13261 gcctgtataa tccggcaacg cttttggctg accgtcacta atattaagca acagtttggt
    13321 tgttgcggat tgtctgttta atttttctgc tgctaaacga agtgcagcac catcacgatt
    13381 gttttgcctt ggtttcatag tgactaattt ttcatttaac cattgaaatc cgtcttcaaa
    13441 ttctttataa gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg tatctccgta
    13501 aatcaacaat gggatatcga ctttcttaga aaattcggca atggcaatcg ctgctttttt
    13561 tgccgcttcg atccgatcat cccgtatcat tgacccagat tcatcgatcc gtacagctac
    13621 cgctaaagaa ggttgctcat gtggcgggtt ctttttgtca aaatttctca aatcaccata
    13681 tgctactttg cttgcattga accgctgacc ttcgtatttt cctttttggt acgtctctgt
    13741 ttgttcatta tccagtaact ctaaaatttg ccggctaagt gattctacta ttggcattac
    13801 ttgcatgtgt agctcacgtg cttccggcgt ttttccttgg aaagctggtc ggtgaacgat
    13861 gagaccttct ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt tattcagttg
    13921 cttcttgatt tccttatcat gtgcttccat gtcagaagca gacatttctt gagaatcatc
    13981 tcctgtttgt tgttcttttt gattttttcc ttcgatatcg ctactgcgtt ctttttgcat
    14041 ttgaggagga atctcgtttt tttctattgt ttcttcttgt ttctcttctc cctcatttgg
    14101 ttcttcagca gacaacgatt tcttttcatc taagcgcaga atttcttcag cattttcagt
    14161 gatgtttcgt tcatttgctt cttcggcgct aatggttctt tcttttgtat gctcagattg
    14221 aggatgaccc catttttctt ttctcaatat gtcctgaagt tccagtagca atccactgtc
    14281 agacaaagct tgttcaagaa ttgccaattc atctgaatca gtggataatt tatacaaaac
    14341 ttttggataa atcgactgga gtaaatcttg tccttgctta taggtatttg cccaataaaa
    14401 ataagaacgc attcctgcta cacctttgat ggcgttagct tttgcagttt catccaacaa
    14461 acggataatc tctgccatct ttaagagaag ttcttgtttt tggatcatag ttttcgccat
    14521 cgcacgttct gccatgactt ctaaggcagg aagatccatt ttttccgcat gttgcatccg
    14581 atctcttaaa gattcattta atgggcgatt tccttggtaa tttcgatttg tagtgatcac
    14641 aatgacgcaa tctggatggc gacgaatgat tcctgtcggt aaattcaaca tcccatttgg
    14701 ttcgagcgct gagttaagtg ccacaagaac agaggcatct cggatgacag tcggctcttg
    14761 aatctctaat aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt aatagctgta
    14821 atggattgca tctggataat cagcttcgat cctttgaact agctctgcta atttttcttt
    14881 agcagaaaaa ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag cttcaagtgt
    14941 atctgtttgg taaatagctt ctagtaattc ttggtcttgt tccttattag aatctactac
    15001 aggtaataag gctccgaaaa catctgattt gtccatatct gcaaaacagg tcacttttgt
    15061 atagggaagt tgcagatcag cagatagtgc tttggctaat tgtgtcttcc ccgacccggc
    15121 atctccttcc aaaagtacgt tactgatttt catttcagga tcttgccaat tccctttgat
    15181 ttcggaggcg atccgccatt cttctgcact tgtccgatgg cttttcggtt tcttccagat
    15241 catttgcttt tctagccgtg aaaaggttcg gttttctgtt agtgagaaat tctccatgct
    15301 tctcttcccc ttttgatcat ctctgtctat taatatccta gctcatctaa aagacgtttt
    15361 aaaatacgga atcgttcccc aatcagttct ccgtcttttt gtagtgtatc gtaatacaaa
    15421 atgatgtctt catcgatctt tggatttttg gtatctactt ctaccgtcat tcggttccct
    15481 tgtaatccaa tgatgtctac gactggattt tctggatcat aaaatttttc ataacgataa
    15541 gcatcttgga agtataaact tccttgcgca gctaagatcg ctaaatctaa aatacgtgtg
    15601 attggtaatt cttccgactg tcgtgaccat ttttctccag tatgtcgcca taccttgcct
    15661 gagatatcga cttttccacg atcattccac tgagcaaggc ccagtgaaag cccttttgca
    15721 tccatattta atgcatttct tccatcaact ttatcatagt cttctacaac gattactggt
    15781 ttatgtttta aatttgttgg tatttccatt attttaactc ctcatccttt tatttttagt
    15841 aaataagtaa attagtagtt tactcattta ctaaactata tgcgacatta tagaggattg
    15901 aagtgaggat tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc atctcgttct
    15961 tttacacgct cattagtaac atgtagaaag tccaagatga tgttggaata ttttggctct
    16021 atactttttg atgttggtga acttcaccaa catctttttt gt
//
LOCUS       DAZKMZ010000053        15892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000053 DAZKMZ010000000
VERSION     DAZKMZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.053"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1182
                     /locus_tag="ACG8M5_001856"
     CDS             157..1182
                     /locus_tag="ACG8M5_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359106.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1777..5430)
                     /gene="rpoC"
                     /locus_tag="ACG8M5_001857"
     CDS             complement(1777..5430)
                     /gene="rpoC"
                     /locus_tag="ACG8M5_001857"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0359107.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5509..9126)
                     /gene="rpoB"
                     /locus_tag="ACG8M5_001858"
     CDS             complement(5509..9126)
                     /gene="rpoB"
                     /locus_tag="ACG8M5_001858"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0359108.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9854..10822
                     /locus_tag="ACG8M5_001859"
     CDS             9854..10822
                     /locus_tag="ACG8M5_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0359109.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10840..11745
                     /locus_tag="ACG8M5_001860"
     CDS             10840..11745
                     /locus_tag="ACG8M5_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359110.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11742..12548
                     /locus_tag="ACG8M5_001861"
     CDS             11742..12548
                     /locus_tag="ACG8M5_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359111.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12705..13673
                     /locus_tag="ACG8M5_001862"
     CDS             12705..13673
                     /locus_tag="ACG8M5_001862"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0359112.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(13990..14514)
                     /locus_tag="ACG8M5_001863"
     CDS             complement(13990..14514)
                     /locus_tag="ACG8M5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0359113.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14801..>15892
                     /gene="scm"
                     /locus_tag="ACG8M5_001864"
     CDS             14801..>15892
                     /gene="scm"
                     /locus_tag="ACG8M5_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0359114.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTT"
BASE COUNT         4597 a         3386 c         3069 g         4840 t
ORIGIN      
        1 agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa
       61 gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta
      121 ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt
      181 gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat
      241 atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg
      301 agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat
      361 cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca atccatttct
      421 gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa
      481 gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta
      541 agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg
      601 gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc
      661 caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt
      721 ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc
      781 ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta
      841 gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt
      901 ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag
      961 cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt
     1021 tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa
     1081 cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc
     1141 gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa
     1201 cagaaacact gaggataagt cagttactct gttctttttt caatcactca tccctcgtta
     1261 ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga
     1321 taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc
     1381 acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc
     1441 atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt
     1501 tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct
     1561 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
     1621 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
     1681 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
     1741 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
     1801 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
     1861 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
     1921 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
     1981 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
     2041 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
     2101 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
     2161 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
     2221 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
     2281 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
     2341 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
     2401 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
     2461 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
     2521 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
     2581 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
     2641 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
     2701 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
     2761 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
     2821 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
     2881 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
     2941 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
     3001 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
     3061 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
     3121 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
     3181 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
     3241 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
     3301 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
     3361 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
     3421 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
     3481 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
     3541 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
     3601 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
     3661 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
     3721 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
     3781 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
     3841 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
     3901 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgggaagg
     3961 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
     4021 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
     4081 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
     4141 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
     4201 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
     4261 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
     4321 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
     4381 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
     4441 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
     4501 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
     4561 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
     4621 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
     4681 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
     4741 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
     4801 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
     4861 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
     4921 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
     4981 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
     5041 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
     5101 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
     5161 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
     5221 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
     5281 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
     5341 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
     5401 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
     5461 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
     5521 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
     5581 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
     5641 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
     5701 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
     5761 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
     5821 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
     5881 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
     5941 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
     6001 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
     6061 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
     6121 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
     6181 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
     6241 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
     6301 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
     6361 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
     6421 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
     6481 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
     6541 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
     6601 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
     6661 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
     6721 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
     6781 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
     6841 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
     6901 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
     6961 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
     7021 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
     7081 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
     7141 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
     7201 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
     7261 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
     7321 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
     7381 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
     7441 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
     7501 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
     7561 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
     7621 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
     7681 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
     7741 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
     7801 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
     7861 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
     7921 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
     7981 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
     8041 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
     8101 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
     8161 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
     8221 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
     8281 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
     8341 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
     8401 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
     8461 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
     8521 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
     8581 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
     8641 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
     8701 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
     8761 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
     8821 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
     8881 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
     8941 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
     9001 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
     9061 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
     9121 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
     9181 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
     9241 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
     9301 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
     9361 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
     9421 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
     9481 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
     9541 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
     9601 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
     9661 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
     9721 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
     9781 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
     9841 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
     9901 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
     9961 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    10021 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    10081 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    10141 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    10201 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    10261 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    10321 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    10381 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    10441 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    10501 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    10561 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    10621 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggta aacaaacagg
    10681 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    10741 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    10801 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    10861 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    10921 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    10981 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    11041 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    11101 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    11161 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    11221 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    11281 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    11341 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    11401 tatatgattt ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc
    11461 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    11521 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    11581 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    11641 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    11701 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    11761 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    11821 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    11881 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    11941 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    12001 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    12061 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    12121 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    12181 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    12241 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    12301 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    12361 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    12421 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    12481 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    12541 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    12601 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    12661 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    12721 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    12781 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    12841 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    12901 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    12961 cttctgaatc tacgatgaaa gatgtcaaac tggcagcagc tcaaggagat tctgtttcga
    13021 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    13081 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    13141 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    13201 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    13261 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    13321 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    13381 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    13441 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    13501 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    13561 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    13621 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    13681 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    13741 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    13801 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    13861 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    13921 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    13981 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    14041 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    14101 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    14161 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    14221 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    14281 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    14341 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    14401 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    14461 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    14521 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    14581 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    14641 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    14701 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    14761 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    14821 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    14881 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    14941 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    15001 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    15061 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    15121 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    15181 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    15241 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    15301 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    15361 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    15421 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    15481 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    15541 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    15601 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    15661 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    15721 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    15781 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    15841 cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta cc
//
LOCUS       DAZKMZ010000054        15546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000054 DAZKMZ010000000
VERSION     DAZKMZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.054"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..743)
                     /locus_tag="ACG8M5_001865"
     CDS             complement(30..743)
                     /locus_tag="ACG8M5_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359115.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            918..2342
                     /locus_tag="ACG8M5_001866"
     CDS             918..2342
                     /locus_tag="ACG8M5_001866"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0359116.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2641..3348
                     /locus_tag="ACG8M5_001867"
     CDS             2641..3348
                     /locus_tag="ACG8M5_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0359117.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3335..3658
                     /locus_tag="ACG8M5_001868"
     CDS             3335..3658
                     /locus_tag="ACG8M5_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0359118.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3747..4784)
                     /locus_tag="ACG8M5_001869"
     CDS             complement(3747..4784)
                     /locus_tag="ACG8M5_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0359119.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5105..6088)
                     /locus_tag="ACG8M5_001870"
     CDS             complement(5105..6088)
                     /locus_tag="ACG8M5_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0359120.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6503..7273)
                     /locus_tag="ACG8M5_001871"
     CDS             complement(6503..7273)
                     /locus_tag="ACG8M5_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359121.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7266..8201)
                     /locus_tag="ACG8M5_001872"
     CDS             complement(7266..8201)
                     /locus_tag="ACG8M5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359122.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8517..10166)
                     /locus_tag="ACG8M5_001873"
     CDS             complement(8517..10166)
                     /locus_tag="ACG8M5_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0359123.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10283..12886)
                     /locus_tag="ACG8M5_001874"
     CDS             complement(10283..12886)
                     /locus_tag="ACG8M5_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0359124.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12898..13947)
                     /locus_tag="ACG8M5_001875"
     CDS             complement(12898..13947)
                     /locus_tag="ACG8M5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0359125.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13973..14287)
                     /locus_tag="ACG8M5_001876"
     CDS             complement(13973..14287)
                     /locus_tag="ACG8M5_001876"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0359126.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14303..14749)
                     /locus_tag="ACG8M5_001877"
     CDS             complement(14303..14749)
                     /locus_tag="ACG8M5_001877"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0359127.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14750..>15546)
                     /locus_tag="ACG8M5_001878"
     CDS             complement(14750..>15546)
                     /locus_tag="ACG8M5_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0359128.1"
                     /translation="ERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTS
                     KLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLG
                     ADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKE
                     GILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFA
                     LNEKVKPELNKIYETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4608 a         3202 c         2591 g         5145 t
ORIGIN      
        1 taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca
       61 aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca
      121 acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca
      181 aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt
      241 tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg
      301 actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt
      361 cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc
      421 gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg
      481 ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact
      541 tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct
      601 ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt
      661 ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc
      721 atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata
      781 ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata
      841 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
      901 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
      961 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
     1021 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
     1081 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
     1141 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
     1201 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
     1261 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
     1321 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
     1381 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
     1441 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
     1501 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
     1561 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
     1621 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
     1681 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
     1741 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
     1801 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
     1861 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
     1921 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
     1981 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
     2041 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
     2101 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
     2161 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
     2221 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
     2281 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
     2341 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
     2401 aataacaaat actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca
     2461 aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact
     2521 acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag
     2581 aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
     2641 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt
     2701 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg
     2761 gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc
     2821 atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc
     2881 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
     2941 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
     3001 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
     3061 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
     3121 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
     3181 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
     3241 attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca
     3301 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
     3361 gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact
     3421 gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
     3481 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
     3541 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
     3601 cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat
     3661 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
     3721 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
     3781 cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
     3841 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
     3901 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
     3961 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
     4021 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca
     4081 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
     4141 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
     4201 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
     4261 agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg
     4321 cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc
     4381 aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg
     4441 acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct
     4501 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact
     4561 ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga
     4621 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     4681 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac
     4741 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     4801 cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt
     4861 tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg
     4921 ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa
     4981 ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg
     5041 ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa
     5101 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc
     5161 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
     5221 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
     5281 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
     5341 tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca
     5401 tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat
     5461 ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag
     5521 ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
     5581 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
     5641 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa
     5701 tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta
     5761 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
     5821 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
     5881 gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga
     5941 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
     6001 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
     6061 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
     6121 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
     6181 tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca
     6241 caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac
     6301 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
     6361 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
     6421 acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa
     6481 cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc
     6541 aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg
     6601 gcacatcgat tgttccaagg aatccgtaac ggaaaccgga gatcatataa acgatcggat
     6661 tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac
     6721 ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact
     6781 gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg
     6841 taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg
     6901 ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga
     6961 tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag
     7021 agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac
     7081 caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca
     7141 tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga
     7201 cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat
     7261 aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt
     7321 tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc
     7381 tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca
     7441 tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca
     7501 aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc
     7561 agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa
     7621 tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc
     7681 tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt
     7741 tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt
     7801 gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc
     7861 atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc
     7921 gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg
     7981 actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact
     8041 aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca
     8101 tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca
     8161 taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat
     8221 agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc
     8281 gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag
     8341 tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa
     8401 caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     8461 acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat
     8521 cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct
     8581 actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
     8641 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
     8701 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
     8761 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
     8821 cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg
     8881 gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
     8941 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
     9001 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
     9061 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
     9121 ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
     9181 ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt
     9241 gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
     9301 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
     9361 acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat
     9421 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc
     9481 agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
     9541 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg
     9601 attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga
     9661 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
     9721 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
     9781 ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct
     9841 tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag
     9901 gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg
     9961 atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct
    10021 tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt
    10081 aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt
    10141 aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt
    10201 atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt
    10261 ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa taatcatagg
    10321 gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa
    10381 ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc
    10441 cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa
    10501 tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta
    10561 acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt
    10621 tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa
    10681 aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt
    10741 catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt
    10801 cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca tactctttta
    10861 aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga
    10921 atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat
    10981 caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta
    11041 aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc
    11101 cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt tcttccattt
    11161 cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa
    11221 ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt
    11281 ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca
    11341 aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa
    11401 aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta
    11461 atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat
    11521 ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa
    11581 tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg
    11641 gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac
    11701 tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat
    11761 cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga
    11821 ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag
    11881 cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac
    11941 catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt
    12001 taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt
    12061 aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt
    12121 ctacataaga aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct
    12181 ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa
    12241 tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg
    12301 caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg
    12361 gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg
    12421 gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat
    12481 gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca
    12541 ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg
    12601 attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc
    12661 ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat
    12721 cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat
    12781 ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc
    12841 attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta
    12901 ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc
    12961 attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat
    13021 attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa
    13081 aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa
    13141 aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat
    13201 agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc
    13261 taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa
    13321 gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc
    13381 catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga
    13441 catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac
    13501 ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt
    13561 tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca
    13621 agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc
    13681 agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga
    13741 aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa
    13801 tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa
    13861 ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat
    13921 ttctcttcct tgtttcttga attccattag taactccccc acgatatatt ttttatttgt
    13981 taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc
    14041 ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg
    14101 caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca
    14161 tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat
    14221 tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt
    14281 ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc
    14341 tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta
    14401 aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat
    14461 caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca
    14521 ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc
    14581 ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca
    14641 caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg
    14701 catttaaatc tatcactata ttatttatat tgattttttc actgatcatt tataagcctt
    14761 ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt gctaataact
    14821 catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca ggttttactt
    14881 tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa tcaattcctt
    14941 ctggattaac gtagacacag attactggct tttttataaa atctacttcg atatgcggca
    15001 tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct ccctctagta
    15061 tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt aaaacgttca
    15121 cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa
    15181 aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg acatctgccc
    15241 ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc ggaatcgtgc
    15301 ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt agtacatctt
    15361 ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat tttgatgttc
    15421 catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa
    15481 gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt atacagaatc
    15541 tctctt
//
LOCUS       DAZKMZ010000055        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000055 DAZKMZ010000000
VERSION     DAZKMZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.055"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG8M5_001879"
     CDS             2..1021
                     /locus_tag="ACG8M5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0359129.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG8M5_001880"
     CDS             1018..1785
                     /locus_tag="ACG8M5_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0359130.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8M5_001881"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG8M5_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0359131.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG8M5_001882"
     CDS             3750..4070
                     /locus_tag="ACG8M5_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0359132.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG8M5_001883"
     CDS             4070..4420
                     /locus_tag="ACG8M5_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0359133.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8M5_001884"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG8M5_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0359134.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8M5_001885"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG8M5_001885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0359135.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG8M5_001886"
     CDS             6414..7439
                     /locus_tag="ACG8M5_001886"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0359136.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG8M5_001887"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG8M5_001887"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0359137.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG8M5_001888"
     CDS             8517..8957
                     /locus_tag="ACG8M5_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0359138.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG8M5_001889"
     CDS             8960..9562
                     /locus_tag="ACG8M5_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0359139.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG8M5_001890"
     CDS             complement(9625..10728)
                     /locus_tag="ACG8M5_001890"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0359140.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8M5_001891"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG8M5_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0359141.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG8M5_001892"
     CDS             12294..12689
                     /locus_tag="ACG8M5_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359142.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG8M5_001893"
     CDS             12689..12940
                     /locus_tag="ACG8M5_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359143.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG8M5_001894"
     CDS             12983..13378
                     /locus_tag="ACG8M5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359144.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG8M5_001895"
     CDS             13375..14631
                     /locus_tag="ACG8M5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0359145.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG8M5_001896"
     CDS             14643..14966
                     /locus_tag="ACG8M5_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359146.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG8M5_001897"
     CDS             14959..15225
                     /locus_tag="ACG8M5_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359147.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKMZ010000056        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000056 DAZKMZ010000000
VERSION     DAZKMZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.056"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8M5_001898"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8M5_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8M5_001899"
     CDS             706..1734
                     /locus_tag="ACG8M5_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0359148.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8M5_001900"
     CDS             1731..2489
                     /locus_tag="ACG8M5_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0359149.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8M5_001901"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8M5_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0359150.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8M5_001902"
     CDS             3376..4629
                     /locus_tag="ACG8M5_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0359151.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8M5_001903"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8M5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8M5_001904"
     CDS             5300..5626
                     /locus_tag="ACG8M5_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0359152.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8M5_001905"
     CDS             5694..6407
                     /locus_tag="ACG8M5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0359153.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M5_001906"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M5_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8M5_001907"
     CDS             7690..9555
                     /locus_tag="ACG8M5_001907"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0359154.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8M5_001908"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8M5_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8M5_001909"
     CDS             11120..11821
                     /locus_tag="ACG8M5_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359155.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8M5_001910"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8M5_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8M5_001911"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8M5_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0359156.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG8M5_001912"
     CDS             13307..13882
                     /locus_tag="ACG8M5_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0359157.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG8M5_001913"
     CDS             13882..14322
                     /locus_tag="ACG8M5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0359158.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG8M5_001914"
     CDS             14338..15039
                     /locus_tag="ACG8M5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0359159.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKMZ010000057        14933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000057 DAZKMZ010000000
VERSION     DAZKMZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.057"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(500..1066)
                     /locus_tag="ACG8M5_001915"
     CDS             complement(500..1066)
                     /locus_tag="ACG8M5_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0359160.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1066..2022)
                     /locus_tag="ACG8M5_001916"
     CDS             complement(1066..2022)
                     /locus_tag="ACG8M5_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0359161.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2022..2678)
                     /locus_tag="ACG8M5_001917"
     CDS             complement(2022..2678)
                     /locus_tag="ACG8M5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0359162.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3048..5066
                     /locus_tag="ACG8M5_001918"
     CDS             3048..5066
                     /locus_tag="ACG8M5_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0359163.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5136..5342
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5493..7907
                     /gene="leuS"
                     /locus_tag="ACG8M5_001919"
     CDS             5493..7907
                     /gene="leuS"
                     /locus_tag="ACG8M5_001919"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0359164.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8255..9808
                     /locus_tag="ACG8M5_001920"
     CDS             8255..9808
                     /locus_tag="ACG8M5_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0359165.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10189..11973)
                     /locus_tag="ACG8M5_001921"
     CDS             complement(10189..11973)
                     /locus_tag="ACG8M5_001921"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359166.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11988..13709)
                     /locus_tag="ACG8M5_001922"
     CDS             complement(11988..13709)
                     /locus_tag="ACG8M5_001922"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359167.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13809..14570
                     /locus_tag="ACG8M5_001923"
     CDS             13809..14570
                     /locus_tag="ACG8M5_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0359168.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4860 a         2743 c         2956 g         4374 t
ORIGIN      
        1 tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata aattcttcca
       61 tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa agcgatattt
      121 gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat atatgaatga
      181 catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg ccattctttt
      241 atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg aataaagagg
      301 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat
      361 gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc gtgaataaag
      421 aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa acatatcatt
      481 tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata ggcggctggt
      541 tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc agtttggaac
      601 aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag tagacgacaa
      661 ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag
      721 tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga ttgaaaatcc
      781 ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa
      841 acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta gcatcgattg
      901 cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt tctgctagaa
      961 aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct ggttgcaaga
     1021 tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga cgctcacctt
     1081 tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt catcaattgc
     1141 attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat cccacggcgc
     1201 atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct ggtcgcaaat
     1261 gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa
     1321 cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga tatctgaatc
     1381 taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg gtaatccgtt
     1441 gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc catctaaata
     1501 agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa
     1561 tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat caactacttc
     1621 atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg gtaaattgac
     1681 tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg taaaattttg
     1741 gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt cgatttcttt
     1801 ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg aaccagatac
     1861 actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac agtcaaatcc
     1921 tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag cgtaattcca
     1981 agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc cttctttctg
     2041 taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag gcacagccaa
     2101 gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc cagataaatg
     2161 cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa tgtgcgtaag
     2221 gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag aatcatactg
     2281 cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa
     2341 ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc aattacaata
     2401 aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat ccataaagcc
     2461 gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat aaagagttgg
     2521 tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg atcgaaacca
     2581 gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag
     2641 aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct ttgaagcaat
     2701 gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa accaatgtat
     2761 gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg gaaagacatt
     2821 ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga aatcctttta
     2881 aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca ttacggtaaa
     2941 ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa atattaagag
     3001 aagttataat tattaataat agataaattg aagttggagg atagaagatg gaaaacattg
     3061 caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg
     3121 cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta gtacagccgg
     3181 ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat acagcggcat
     3241 ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat ctatatgcat
     3301 cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag
     3361 caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc gtctacatgg
     3421 atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc cgacaatatc
     3481 cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact tcatttggca
     3541 acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca gatgcgacag
     3601 cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta aataatatca
     3661 tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca ggcggtggag
     3721 cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca agcaattctg
     3781 gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct ggtgattctg
     3841 tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa tggaacaaca
     3901 tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc caggaaaatg
     3961 gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct ggtacatcaa
     4021 acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct gtttggaaga
     4081 tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat atcaagaaca
     4141 atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct aatgctggag
     4201 caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc tcaagttctt
     4261 ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt tggagcgtct
     4321 caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc caaaataact
     4381 ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac agcacgtcag
     4441 gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact ggcagcagtt
     4501 cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa
     4561 acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac ttcatttatc
     4621 caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca ggttcaacta
     4681 cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca tcatccactg
     4741 gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc
     4801 atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc atctatccag
     4861 gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa tcagttactg
     4921 gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct gacagtcatg
     4981 gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt atttatccag
     5041 gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga ttgaattcca
     5101 caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa ggaagtagta
     5161 aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat gcttttgaac
     5221 tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga cggccgttac
     5281 aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat
     5341 aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg agaaaacaaa
     5401 gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa
     5461 caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga
     5521 aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa
     5581 agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag
     5641 ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat
     5701 acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc
     5761 tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc
     5821 aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg
     5881 agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg
     5941 aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag
     6001 tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat
     6061 ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt
     6121 ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata
     6181 ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca cgaccagata
     6241 ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta
     6301 cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag
     6361 atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa
     6421 tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat
     6481 acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc
     6541 aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt
     6601 acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag
     6661 caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct
     6721 atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca
     6781 tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc
     6841 ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg
     6901 agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa
     6961 tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa
     7021 aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg
     7081 gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg
     7141 atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct
     7201 taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg
     7261 tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag
     7321 atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg
     7381 tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg
     7441 atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa
     7501 atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag
     7561 tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc
     7621 ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac
     7681 catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag
     7741 taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag
     7801 aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa
     7861 aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc
     7921 aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct
     7981 cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg
     8041 ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc
     8101 atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga
     8161 gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg
     8221 cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga
     8281 agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat
     8341 gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt
     8401 cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa
     8461 tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct
     8521 tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca
     8581 aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt
     8641 tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct
     8701 gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa
     8761 cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc
     8821 tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt
     8881 agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc
     8941 tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg
     9001 gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa
     9061 acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt
     9121 tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat
     9181 tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct catggtttat
     9241 ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca
     9301 aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg
     9361 caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat
     9421 ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca
     9481 gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag
     9541 tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga
     9601 aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag
     9661 cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa
     9721 aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca
     9781 actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac
     9841 cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg
     9901 tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta
     9961 caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg
    10021 attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt
    10081 atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc
    10141 gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg
    10201 aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt
    10261 tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt
    10321 tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg
    10381 ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac
    10441 aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa
    10501 cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg
    10561 atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc
    10621 gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc
    10681 aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat
    10741 ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc
    10801 accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt
    10861 catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt
    10921 cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat
    10981 ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc
    11041 ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat
    11101 cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag
    11161 ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa
    11221 agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt
    11281 gaatgttttg accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc
    11341 cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac
    11401 aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa
    11461 cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt
    11521 caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac
    11581 ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg
    11641 caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt
    11701 gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt
    11761 gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc
    11821 gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca
    11881 tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa
    11941 agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt
    12001 gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt
    12061 ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg
    12121 cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat
    12181 gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct
    12241 ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa
    12301 ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat
    12361 tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc
    12421 aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg
    12481 gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct
    12541 atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc
    12601 ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact
    12661 ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga
    12721 tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc
    12781 agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata
    12841 gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca
    12901 ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa
    12961 acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg
    13021 atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga
    13081 ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc
    13141 cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa
    13201 acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa
    13261 cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa
    13321 aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat
    13381 ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc
    13441 tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact
    13501 gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact
    13561 gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct
    13621 aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt
    13681 ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg
    13741 attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt
    13801 gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa
    13861 aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga
    13921 atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata
    13981 gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt
    14041 tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac
    14101 aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg
    14161 aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag
    14221 atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc
    14281 cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat
    14341 ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga
    14401 aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg
    14461 aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa
    14521 acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg
    14581 tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat
    14641 tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg
    14701 aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc
    14761 ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact
    14821 gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga
    14881 taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt aca
//
LOCUS       DAZKMZ010000058        14624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000058 DAZKMZ010000000
VERSION     DAZKMZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.058"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..815
                     /locus_tag="ACG8M5_001924"
     CDS             3..815
                     /locus_tag="ACG8M5_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0359169.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="ACG8M5_001925"
     CDS             812..1612
                     /locus_tag="ACG8M5_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0359170.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="ACG8M5_001926"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="ACG8M5_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0359171.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="ACG8M5_001927"
     CDS             3109..4818
                     /locus_tag="ACG8M5_001927"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0359172.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9388
                     /locus_tag="ACG8M5_001928"
     CDS             5030..9388
                     /locus_tag="ACG8M5_001928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0359173.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9533..10006
                     /gene="rimP"
                     /locus_tag="ACG8M5_001929"
     CDS             9533..10006
                     /gene="rimP"
                     /locus_tag="ACG8M5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0359174.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10029..11204
                     /gene="nusA"
                     /locus_tag="ACG8M5_001930"
     CDS             10029..11204
                     /gene="nusA"
                     /locus_tag="ACG8M5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0359175.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11226..11519
                     /locus_tag="ACG8M5_001931"
     CDS             11226..11519
                     /locus_tag="ACG8M5_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0359176.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11516..11827
                     /locus_tag="ACG8M5_001932"
     CDS             11516..11827
                     /locus_tag="ACG8M5_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0359177.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11840..14146
                     /gene="infB"
                     /locus_tag="ACG8M5_001933"
     CDS             11840..14146
                     /gene="infB"
                     /locus_tag="ACG8M5_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0359178.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14173..14520
                     /gene="rbfA"
                     /locus_tag="ACG8M5_001934"
     CDS             14173..14520
                     /gene="rbfA"
                     /locus_tag="ACG8M5_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0359179.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4982 a         2598 c         3255 g         3789 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5701 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5761 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5821 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     5881 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     5941 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6001 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6061 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6121 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6181 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6241 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6301 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6361 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6421 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6481 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6541 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6601 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6661 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6721 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6781 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6841 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     6901 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     6961 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7021 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7081 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7141 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7201 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7261 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7321 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7381 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7441 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7501 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7561 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7621 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7681 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7741 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7801 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7861 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     7921 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     7981 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8041 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8101 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8161 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8221 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8281 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8341 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8401 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8461 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8521 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8581 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8641 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8701 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8761 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8821 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     8881 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     8941 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9001 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9061 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9121 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9181 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9241 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9301 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9361 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9421 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9481 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9541 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9601 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9661 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9721 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9781 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9841 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
     9901 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
     9961 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10021 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10081 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10141 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10201 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10261 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10321 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10381 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10441 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10501 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10561 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10621 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10681 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10741 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10801 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10861 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    10921 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    10981 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11041 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11101 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11161 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11221 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11281 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11341 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11401 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11461 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11521 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11581 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11641 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11701 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11761 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11821 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    11881 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    11941 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12001 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12061 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12121 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12181 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12241 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12301 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12361 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12421 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12481 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12541 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12601 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12661 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12721 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12781 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12841 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    12901 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    12961 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13021 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13081 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13141 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13201 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13261 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13321 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13381 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13441 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13501 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13561 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13621 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13681 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13741 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13801 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13861 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    13921 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    13981 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14041 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14101 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14161 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14221 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14281 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14341 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14401 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14461 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14521 tcaaaaactc ccgcaaatcc tgtgaaatca ggctctttgt caataaggac tgatgagttg
    14581 tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DAZKMZ010000059        13959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000059 DAZKMZ010000000
VERSION     DAZKMZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.059"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG8M5_001935"
     CDS             <1..135
                     /locus_tag="ACG8M5_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0359180.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8M5_001936"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG8M5_001936"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0359181.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG8M5_001937"
     CDS             complement(2412..2663)
                     /locus_tag="ACG8M5_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0359182.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8M5_001938"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG8M5_001938"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0359183.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG8M5_001939"
     CDS             3613..3831
                     /locus_tag="ACG8M5_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359184.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="ACG8M5_001940"
     CDS             complement(4120..4632)
                     /locus_tag="ACG8M5_001940"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0359185.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8M5_001941"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="ACG8M5_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0359186.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="ACG8M5_001942"
     CDS             complement(7085..7528)
                     /locus_tag="ACG8M5_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0359187.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="ACG8M5_001943"
     CDS             complement(7529..8317)
                     /locus_tag="ACG8M5_001943"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0359188.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8M5_001944"
     CDS             complement(8314..9255)
                     /gene="rnz"
                     /locus_tag="ACG8M5_001944"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0359189.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="ACG8M5_001945"
     CDS             complement(9432..10019)
                     /locus_tag="ACG8M5_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0359190.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8M5_001946"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="ACG8M5_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0359191.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="ACG8M5_001947"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="ACG8M5_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0359192.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="ACG8M5_001948"
     CDS             complement(12936..13871)
                     /locus_tag="ACG8M5_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0359193.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAZKMZ010000060        13839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000060 DAZKMZ010000000
VERSION     DAZKMZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.060"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..364
                     /locus_tag="ACG8M5_001949"
     CDS             170..364
                     /locus_tag="ACG8M5_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359194.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            633..932
                     /locus_tag="ACG8M5_001950"
     CDS             633..932
                     /locus_tag="ACG8M5_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359195.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1029..1463)
                     /locus_tag="ACG8M5_001951"
     CDS             complement(1029..1463)
                     /locus_tag="ACG8M5_001951"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0359196.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1695..2405)
                     /locus_tag="ACG8M5_001952"
     CDS             complement(1695..2405)
                     /locus_tag="ACG8M5_001952"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0359197.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2395..3255)
                     /locus_tag="ACG8M5_001953"
     CDS             complement(2395..3255)
                     /locus_tag="ACG8M5_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0359198.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3260..3898)
                     /locus_tag="ACG8M5_001954"
     CDS             complement(3260..3898)
                     /locus_tag="ACG8M5_001954"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0359199.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3913..4212)
                     /locus_tag="ACG8M5_001955"
     CDS             complement(3913..4212)
                     /locus_tag="ACG8M5_001955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0359200.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4255..5739)
                     /locus_tag="ACG8M5_001956"
     CDS             complement(4255..5739)
                     /locus_tag="ACG8M5_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0359201.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5759..6220)
                     /locus_tag="ACG8M5_001957"
     CDS             complement(5759..6220)
                     /locus_tag="ACG8M5_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359202.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="ACG8M5_001958"
     CDS             complement(6238..7305)
                     /gene="ulaG"
                     /locus_tag="ACG8M5_001958"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0359203.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7523..8293
                     /locus_tag="ACG8M5_001959"
     CDS             7523..8293
                     /locus_tag="ACG8M5_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0359204.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8566..9393)
                     /locus_tag="ACG8M5_001960"
     CDS             complement(8566..9393)
                     /locus_tag="ACG8M5_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359205.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9961..11196
                     /locus_tag="ACG8M5_001961"
     CDS             9961..11196
                     /locus_tag="ACG8M5_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0359206.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12195..12455)
                     /locus_tag="ACG8M5_001962"
     CDS             complement(12195..12455)
                     /locus_tag="ACG8M5_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0359207.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12482..13783)
                     /locus_tag="ACG8M5_001963"
     CDS             complement(12482..13783)
                     /locus_tag="ACG8M5_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359208.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4452 a         2832 c         2178 g         4377 t
ORIGIN      
        1 tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc gagtgaaaaa
       61 aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat cattttcact
      121 tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt
      181 agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata
      241 tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat
      301 aaagaacgag ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa
      361 gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga
      421 ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat
      481 accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga
      541 tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt
      601 tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg
      661 aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca
      721 aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg
      781 caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg
      841 ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat
      901 ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat
      961 actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt
     1021 attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa
     1081 gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt
     1141 ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc
     1201 aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg
     1261 atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc
     1321 ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt
     1381 gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg
     1441 atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata
     1501 atacagttct ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat
     1561 tccttaaaat tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga
     1621 taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc
     1681 ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt
     1741 ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg
     1801 gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc
     1861 ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat
     1921 tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt
     1981 acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga
     2041 gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa
     2101 tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg
     2161 agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc
     2221 accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact
     2281 cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc
     2341 ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa
     2401 gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc
     2461 tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc
     2521 tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac
     2581 atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc
     2641 agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga
     2701 caaattgcct aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc
     2761 taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat
     2821 gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga
     2881 attcattgat ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct
     2941 tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc
     3001 tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg
     3061 tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa
     3121 tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc
     3181 ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat
     3241 cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg
     3301 cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat
     3361 cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa
     3421 agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag
     3481 ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat
     3541 ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt
     3601 tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc
     3661 ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga
     3721 ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta
     3781 agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc
     3841 ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct
     3901 tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat
     3961 ttctttgact gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg
     4021 agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact
     4081 ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat
     4141 tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac
     4201 taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac
     4261 tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt
     4321 tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc
     4381 ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat
     4441 cccgatataa ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc
     4501 tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt
     4561 attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac
     4621 gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc
     4681 aaacaagacc ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc
     4741 aattcctgga acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag
     4801 ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa
     4861 agaagtcgtc ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat
     4921 caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga
     4981 agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat
     5041 ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc
     5101 taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc
     5161 ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc
     5221 taaaatcagt cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag
     5281 taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga
     5341 acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag
     5401 taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac
     5461 agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag
     5521 tccaaccaag attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa
     5581 gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa
     5641 aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt
     5701 tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt
     5761 aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct
     5821 caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt
     5881 ctggatcttt tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt
     5941 cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac
     6001 catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac
     6061 cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat
     6121 cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg
     6181 ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta
     6241 taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata
     6301 aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca
     6361 gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat
     6421 ctcttttgga tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt
     6481 tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc
     6541 tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt
     6601 ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc
     6661 gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc
     6721 ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt
     6781 gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat
     6841 tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca
     6901 attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac
     6961 gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa
     7021 attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat
     7081 gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc
     7141 ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg
     7201 atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc
     7261 tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc
     7321 atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt
     7381 ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat
     7441 aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga
     7501 tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa
     7561 agaaattttg gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc
     7621 ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga
     7681 gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg attttgatca
     7741 atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga
     7801 ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca
     7861 atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga
     7921 taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg
     7981 cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag
     8041 cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga
     8101 aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg
     8161 actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa
     8221 attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt
     8281 agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa
     8341 caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg
     8401 tatccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc
     8461 taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt
     8521 tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt
     8581 ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat
     8641 atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag
     8701 caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca
     8761 taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata
     8821 tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc
     8881 cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc
     8941 agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata
     9001 aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa
     9061 aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt
     9121 ataagattct tggtattttg atacagaatt caatgattga atctcattta tttttttcct
     9181 gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca
     9241 aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt
     9301 tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta
     9361 ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta
     9421 tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga
     9481 ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga
     9541 tttctttttg tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt
     9601 catggcgaga aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata
     9661 atgacgacag cacacctatc aattaatata attattttat ttttttatgt tctcatgttt
     9721 atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat
     9781 tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag
     9841 ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg
     9901 tgttttttag tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc
     9961 atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt
    10021 gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact
    10081 tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg
    10141 aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt
    10201 accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag
    10261 cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag
    10321 attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat
    10381 gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg
    10441 acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa
    10501 aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc
    10561 atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt
    10621 ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag
    10681 cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg
    10741 cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca
    10801 cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa
    10861 tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa
    10921 gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt
    10981 gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa
    11041 ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt
    11101 cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa
    11161 gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta
    11221 atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt
    11281 aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca
    11341 tccttaaaat aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt
    11401 cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga
    11461 aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca
    11521 tcgctttgta gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat
    11581 ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc
    11641 cgatgcatac tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga
    11701 atgttctatg tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat
    11761 tcccagcaca attatttcta tacacgtttc atgatttgaa taaacactca catcatacat
    11821 attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat
    11881 atttgacttg atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt
    11941 tccatttttt cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa
    12001 aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag
    12061 cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt
    12121 taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac
    12181 cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa
    12241 ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc
    12301 ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga
    12361 ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa
    12421 aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa
    12481 cttagacagt ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat
    12541 cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca
    12601 agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg
    12661 tccatgttgg caaaacagta aatctattga ccatttctaa tttgactgca aaataactga
    12721 taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca
    12781 taggagctcc aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac
    12841 gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga
    12901 tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat
    12961 agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga
    13021 tcagccacat tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc
    13081 acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat
    13141 tggttaaagg ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc
    13201 gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg
    13261 caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac
    13321 gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca
    13381 aagaactcat aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag
    13441 gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc
    13501 catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca
    13561 tcgtcatcct aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc
    13621 caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg
    13681 ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg
    13741 gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc
    13801 aataaaatat gaaccattca ttgcatgaca acgcaaaga
//
LOCUS       DAZKMZ010000061        13644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000061 DAZKMZ010000000
VERSION     DAZKMZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.061"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8M5_001964"
     CDS             complement(<1..210)
                     /locus_tag="ACG8M5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0359209.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG8M5_001965"
     CDS             complement(216..446)
                     /locus_tag="ACG8M5_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359210.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG8M5_001966"
     CDS             1063..1725
                     /locus_tag="ACG8M5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359211.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8M5_001967"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8M5_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0359212.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG8M5_001968"
     CDS             5145..5459
                     /locus_tag="ACG8M5_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0359213.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG8M5_001969"
     CDS             5472..5846
                     /locus_tag="ACG8M5_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0359214.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG8M5_001970"
     CDS             5856..6200
                     /locus_tag="ACG8M5_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0359215.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG8M5_001971"
     CDS             6271..7623
                     /locus_tag="ACG8M5_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0359216.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG8M5_001972"
     CDS             7683..8825
                     /locus_tag="ACG8M5_001972"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0359217.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG8M5_001973"
     CDS             8850..9104
                     /locus_tag="ACG8M5_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359218.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8M5_001974"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8M5_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0359219.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG8M5_001975"
     CDS             10541..10678
                     /locus_tag="ACG8M5_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0359220.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG8M5_001976"
     CDS             10683..10904
                     /locus_tag="ACG8M5_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359221.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG8M5_001977"
     CDS             10917..11417
                     /locus_tag="ACG8M5_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0359222.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG8M5_001978"
     CDS             11514..13361
                     /locus_tag="ACG8M5_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0359223.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..>13644
                     /locus_tag="ACG8M5_001979"
     CDS             13364..>13644
                     /locus_tag="ACG8M5_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359224.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDT"
BASE COUNT         4601 a         2193 c         2997 g         3853 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acac
//
LOCUS       DAZKMZ010000062        13441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000062 DAZKMZ010000000
VERSION     DAZKMZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.062"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..643)
                     /locus_tag="ACG8M5_001980"
     CDS             complement(101..643)
                     /locus_tag="ACG8M5_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0359225.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            778..1167
                     /locus_tag="ACG8M5_001981"
     CDS             778..1167
                     /locus_tag="ACG8M5_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359226.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1171..1935
                     /locus_tag="ACG8M5_001982"
     CDS             1171..1935
                     /locus_tag="ACG8M5_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0359227.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2064..3011
                     /locus_tag="ACG8M5_001983"
     CDS             2064..3011
                     /locus_tag="ACG8M5_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359228.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3141..3881
                     /locus_tag="ACG8M5_001984"
     CDS             3141..3881
                     /locus_tag="ACG8M5_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359229.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4172..4840)
                     /locus_tag="ACG8M5_001985"
     CDS             complement(4172..4840)
                     /locus_tag="ACG8M5_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0359230.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4974..5261
                     /locus_tag="ACG8M5_001986"
     CDS             4974..5261
                     /locus_tag="ACG8M5_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359231.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5677..7215
                     /locus_tag="ACG8M5_001987"
     CDS             5677..7215
                     /locus_tag="ACG8M5_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0359232.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7212..8177
                     /locus_tag="ACG8M5_001988"
     CDS             7212..8177
                     /locus_tag="ACG8M5_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359233.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8164..9486
                     /locus_tag="ACG8M5_001989"
     CDS             8164..9486
                     /locus_tag="ACG8M5_001989"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0359234.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9549..9653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9727..10470
                     /locus_tag="ACG8M5_001990"
     CDS             9727..10470
                     /locus_tag="ACG8M5_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359235.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10485..11477
                     /locus_tag="ACG8M5_001991"
     CDS             10485..11477
                     /locus_tag="ACG8M5_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0359236.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11489..12226
                     /locus_tag="ACG8M5_001992"
     CDS             11489..12226
                     /locus_tag="ACG8M5_001992"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359237.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12216..12938
                     /locus_tag="ACG8M5_001993"
     CDS             12216..12938
                     /locus_tag="ACG8M5_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0359238.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13022..>13441)
                     /locus_tag="ACG8M5_001994"
     CDS             complement(13022..>13441)
                     /locus_tag="ACG8M5_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0359239.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4429 a         2296 c         2792 g         3924 t
ORIGIN      
        1 tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta
       61 aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc
      121 tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag
      181 cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa
      241 gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat
      301 aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa
      361 tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga
      421 tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta
      481 aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa
      541 tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa
      601 aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca
      661 tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat
      721 acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt gattaaaatg
      781 aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac
      841 ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg
      901 ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt
      961 ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta
     1021 ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata
     1081 ttatttggta cactgaacct gttccagaca actgttggag catggttgct atggcgatca
     1141 ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc
     1201 catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt
     1261 gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa
     1321 attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa
     1381 cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt
     1441 gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca
     1501 gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat
     1561 atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc
     1621 gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga tttgatttca
     1681 tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc
     1741 ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa
     1801 aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga
     1861 catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa aaatttgata
     1921 aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt
     1981 tattttgggc tataataaac tctgttagga aaatttggta taattgatag taagaatgac
     2041 aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg
     2101 tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga
     2161 aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag
     2221 cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa
     2281 ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt
     2341 attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca
     2401 tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct
     2461 tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag
     2521 caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg
     2581 aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag
     2641 ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata
     2701 tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc
     2761 tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg
     2821 gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga
     2881 tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag
     2941 aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt atcagagaga
     3001 caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta ttttcttaaa
     3061 ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga aaagttccat
     3121 ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt tttcctgcgt
     3181 acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta tttgacggac
     3241 aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa gaatttcatt
     3301 tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat ggattgaagt
     3361 cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat tataccggcc
     3421 attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat tcctctcgtt
     3481 ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac gcatctgttg
     3541 acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca tcgattatcc
     3601 cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg aaaaaaacgg
     3661 atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa agaaatggtg
     3721 agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag cagtcagaag
     3781 aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga ttgatcgatg
     3841 atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac aaatttatat
     3901 tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc tatccgaaag
     3961 tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc aataagtaag
     4021 aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagttta
     4081 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
     4141 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
     4201 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
     4261 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
     4321 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
     4381 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
     4441 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
     4501 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
     4561 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
     4621 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
     4681 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
     4741 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
     4801 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
     4861 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
     4921 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
     4981 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
     5041 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     5101 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     5161 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     5221 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     5281 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     5341 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     5401 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     5461 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     5521 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     5581 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     5641 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     5701 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     5761 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     5821 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     5881 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     5941 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     6001 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     6061 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     6121 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     6181 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     6241 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     6301 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     6361 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     6421 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     6481 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     6541 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     6601 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     6661 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     6721 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     6781 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     6841 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     6901 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     6961 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     7021 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     7081 aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc
     7141 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     7201 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     7261 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     7321 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     7381 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     7441 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     7501 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     7561 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     7621 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     7681 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     7741 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     7801 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     7861 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     7921 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     7981 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     8041 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     8101 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     8161 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     8221 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     8281 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     8341 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     8401 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     8461 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     8521 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     8581 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     8641 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     8701 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     8761 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     8821 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     8881 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     8941 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     9001 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     9061 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     9121 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     9181 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     9241 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     9301 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     9361 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     9421 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     9481 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     9541 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     9601 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     9661 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     9721 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     9781 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     9841 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     9901 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     9961 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
    10021 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
    10081 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
    10141 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
    10201 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
    10261 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
    10321 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
    10381 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
    10441 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
    10501 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
    10561 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
    10621 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
    10681 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
    10741 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
    10801 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
    10861 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
    10921 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
    10981 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
    11041 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
    11101 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
    11161 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
    11221 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
    11281 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
    11341 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
    11401 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
    11461 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
    11521 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
    11581 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
    11641 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
    11701 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
    11761 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
    11821 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
    11881 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
    11941 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
    12001 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
    12061 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
    12121 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
    12181 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
    12241 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
    12301 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
    12361 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
    12421 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
    12481 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
    12541 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
    12601 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
    12661 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
    12721 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
    12781 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
    12841 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
    12901 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
    12961 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
    13021 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
    13081 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
    13141 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
    13201 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
    13261 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
    13321 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
    13381 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
    13441 a
//
LOCUS       DAZKMZ010000063        13429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000063 DAZKMZ010000000
VERSION     DAZKMZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.063"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8M5_001995"
     CDS             complement(<1..914)
                     /locus_tag="ACG8M5_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0359240.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M5_001996"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M5_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0359241.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG8M5_001997"
     CDS             2006..2176
                     /locus_tag="ACG8M5_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359242.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8M5_001998"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG8M5_001998"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0359243.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8M5_001999"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG8M5_001999"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0359244.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8M5_002000"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG8M5_002000"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0359245.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8M5_002001"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG8M5_002001"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0359246.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8M5_002002"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG8M5_002002"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0359247.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG8M5_002003"
     CDS             6751..7752
                     /locus_tag="ACG8M5_002003"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0359248.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG8M5_002004"
     CDS             7749..9110
                     /locus_tag="ACG8M5_002004"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0359249.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG8M5_002005"
     CDS             9070..10221
                     /locus_tag="ACG8M5_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0359250.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG8M5_002006"
     CDS             10196..11365
                     /locus_tag="ACG8M5_002006"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0359251.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG8M5_002007"
     CDS             11540..12919
                     /locus_tag="ACG8M5_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0359252.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG8M5_002008"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG8M5_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2340 c         2924 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt acgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKMZ010000064        13267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000064 DAZKMZ010000000
VERSION     DAZKMZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.064"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..346
                     /locus_tag="ACG8M5_002009"
     CDS             179..346
                     /locus_tag="ACG8M5_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0359253.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(408..2219)
                     /locus_tag="ACG8M5_002010"
     CDS             complement(408..2219)
                     /locus_tag="ACG8M5_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0359254.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2216..2581)
                     /locus_tag="ACG8M5_002011"
     CDS             complement(2216..2581)
                     /locus_tag="ACG8M5_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0359255.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2744..3139)
                     /locus_tag="ACG8M5_002012"
     CDS             complement(2744..3139)
                     /locus_tag="ACG8M5_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0359256.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3157..4119)
                     /locus_tag="ACG8M5_002013"
     CDS             complement(3157..4119)
                     /locus_tag="ACG8M5_002013"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0359257.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4119..4331)
                     /locus_tag="ACG8M5_002014"
     CDS             complement(4119..4331)
                     /locus_tag="ACG8M5_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0359258.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4352..5050)
                     /locus_tag="ACG8M5_002015"
     CDS             complement(4352..5050)
                     /locus_tag="ACG8M5_002015"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0359259.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5070..5612)
                     /locus_tag="ACG8M5_002016"
     CDS             complement(5070..5612)
                     /locus_tag="ACG8M5_002016"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0359260.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5744..6748)
                     /locus_tag="ACG8M5_002017"
     CDS             complement(5744..6748)
                     /locus_tag="ACG8M5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0359261.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6745..7734)
                     /locus_tag="ACG8M5_002018"
     CDS             complement(6745..7734)
                     /locus_tag="ACG8M5_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0359262.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7731..8537)
                     /locus_tag="ACG8M5_002019"
     CDS             complement(7731..8537)
                     /locus_tag="ACG8M5_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359263.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8703..9659
                     /locus_tag="ACG8M5_002020"
     CDS             8703..9659
                     /locus_tag="ACG8M5_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0359264.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9736..10254)
                     /locus_tag="ACG8M5_002021"
     CDS             complement(9736..10254)
                     /locus_tag="ACG8M5_002021"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0359265.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10342..10491)
                     /gene="rpmG"
                     /locus_tag="ACG8M5_002022"
     CDS             complement(10342..10491)
                     /gene="rpmG"
                     /locus_tag="ACG8M5_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0359266.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10719..11165
                     /locus_tag="ACG8M5_002023"
     CDS             10719..11165
                     /locus_tag="ACG8M5_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0359267.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11360..13255)
                     /gene="asnB"
                     /locus_tag="ACG8M5_002024"
     CDS             complement(11360..13255)
                     /gene="asnB"
                     /locus_tag="ACG8M5_002024"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0359268.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4137 a         2808 c         2265 g         4057 t
ORIGIN      
        1 tttatggtga aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa
       61 acatttagta aacattttca aggatttttt gcaaaggttt acaaacaaac gtaaaactag
      121 cctttttttc tcttttgttc cataatagat ataacaaata ataaatgagg tggattcaat
      181 gaaaaaattt gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg
      241 tttggttgct tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat
      301 cgaagaaaac cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa
      361 tacgcatgaa gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc
      421 aatataagtt gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta
      481 gctactgctg cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca
      541 gactggattt cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct
      601 tctgaatgat aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc
      661 ttcaaatttt ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct
      721 tcaatcaaat agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc
      781 ttttgctgaa tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct
      841 gctaattctt tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta
      901 aaccatcccc atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc
      961 tgccggattg gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg
     1021 caattgcctt tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa
     1081 aagggctcat tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg
     1141 attagtgctg gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca
     1201 aaatttttct ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac
     1261 tcaacgtctt ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc
     1321 cattgctcca ggttttcatt tgttagtaac cgtggccatg ccattgctcc gccttgcttt
     1381 tgatttcttc ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt
     1441 tccttgcctg atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt
     1501 gatactgtaa acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca
     1561 ttttctttag gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac
     1621 agtaaaacat ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt
     1681 tcccagtgac cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt
     1741 aaaagataac catcttttct tttgcttact ttttttacta aagaataaac aaactgaata
     1801 gaagctggaa attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa
     1861 catcgagtag attgctgatc tttttccaga cgattgattt cttctataat tagttgctta
     1921 tttttggtta ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag
     1981 ttgggtccct gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact
     2041 tgttgagaaa cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct
     2101 ccaggaccat tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg
     2161 agaatccttt ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt
     2221 tgcatgcctc catttcgata aattttttca ataaaacagc atggttctga tgttcatttt
     2281 ttgcaccata aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa
     2341 tagcttgctt cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag
     2401 caaatcgttc tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt
     2461 tcaaccacag atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca
     2521 cgagtattct atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca
     2581 tagttgtttc cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa
     2641 taaaaaaaat gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct
     2701 aatgattgat tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt
     2761 tgatttttcc agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg
     2821 ctttacgtaa tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga
     2881 caggctggtc ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc
     2941 ttgcacctaa aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg
     3001 cttttctcat tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca
     3061 ttactcgtag atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat
     3121 taattaccca taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga
     3181 ggcagcacca atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc
     3241 tcttacttgc gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc
     3301 tcctgctgcc gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa
     3361 gtttccgcaa gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg
     3421 atcgtcttct tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag
     3481 cattgctttc agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac
     3541 cacacctgta gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa
     3601 cccttcagga tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat
     3661 caaatttccg ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac
     3721 gtctggatcg ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc
     3781 attatcgata gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac
     3841 ttctgtccag tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc
     3901 tggggtaccc atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg
     3961 atgattgatt gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt
     4021 atcgatgctc catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt
     4081 tgttccacct aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg
     4141 ctttcttcaa ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga
     4201 tcaatcaaac ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag
     4261 atgcgtgcac ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat
     4321 aagctctcca tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac
     4381 ccagtaaaat acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt
     4441 gtatcgaata gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc
     4501 caattagccc accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa
     4561 atgtcaacac aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt
     4621 taaaatgaaa ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac
     4681 caccagacag aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc
     4741 tgaacatata aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca
     4801 taaaatacag tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg
     4861 gtgtcactaa acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat
     4921 aataagggat ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc
     4981 ctaaaaaagc ataggtccat aacggtcgtc ttaaccatac acgcaatttt atttgctgtt
     5041 gataattcat gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga
     5101 tcaaagctct ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt
     5161 tttttatgaa aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa
     5221 ccggctctat tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag
     5281 agcttctctg attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca
     5341 tccatttcat aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt
     5401 tgttcttttt ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa
     5461 gtcgtcccta tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt
     5521 cgctgttctt tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct
     5581 ttccggcgta atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat
     5641 ggtataggaa aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa
     5701 aaagaagacc tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt
     5761 gtttttgtct gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa
     5821 ttcctgtagg tacttcccca cttagtagaa tcactctagc aaccatatct gcactaagca
     5881 gaaaaatgga accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga
     5941 ctagcttcgc agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta
     6001 cactaccgct tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt
     6061 taaggcctaa tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat
     6121 aaagtcctcc tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc
     6181 agctcgcacc ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag
     6241 ataattggat cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg
     6301 acaataagat tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac
     6361 agcaagcagc taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat
     6421 agtgcgaaaa gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca
     6481 atatactaga atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc
     6541 caagacaagc tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa
     6601 tcagcgccaa agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa
     6661 taaaaggcgt accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga
     6721 aatagaacgt ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa
     6781 cggaacgcca atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat
     6841 cctcgctgta aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag
     6901 aacataacga tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat
     6961 aaatgaaaca ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa
     7021 aacaatcaac cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa
     7081 atgaatcaaa gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa
     7141 aggaaacaat actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa
     7201 agcaagatcc tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa
     7261 agaactaata gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa
     7321 ctgcaaaaaa ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa
     7381 cacagaaaaa tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc
     7441 acccgcgttg attcctagta agcctgaatc tgctaaagga tttcttgtca ctccttgcat
     7501 caatgcaccg ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa
     7561 acgaatattt cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg
     7621 ccaaaaatct gcttttccca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat
     7681 caaaagaaca aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga
     7741 tcctcctgct cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga
     7801 agcgtgacaa tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact
     7861 tcttcaggtg taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa
     7921 aaacgtgaag catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga
     7981 ttgatttcag tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc
     8041 ggttcatcca agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc
     8101 tgggtttgtc caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta
     8161 gcatccaacg cccactcgat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt
     8221 tgataagggt atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca
     8281 ctagtttgtg ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata
     8341 tcagagttat tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc
     8401 aataaagtag attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga
     8461 atcgtaacag acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct
     8521 gctcttaatc tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct
     8581 gtttcaacat ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc
     8641 gttcgaaata tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag
     8701 aaatgaaatt aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac
     8761 tgagcgcttg ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt
     8821 catccaatag tactcataca gtcacagata cacttggaca taaagtagag attccaaacg
     8881 cgcccaaacg gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac
     8941 ctatcgccca gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa
     9001 aggatgtccc aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg
     9061 atttgctgtt gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca
     9121 aaatcgcgcc aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa
     9181 aagatgtcgg caaagctttg gcaaaagaaa aagaggcaga aaagatcatt tccgattatc
     9241 aaaaatccat caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg
     9301 cagtgttgtg ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa
     9361 gaatcgtata cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa
     9421 aagccacttc tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact
     9481 acatcatcct tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga
     9541 atcttaaagc agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat
     9601 ataacggacc tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat
     9661 tttaactaaa aaaagcatct tcacgattag tcgtgatatg ctttttttct tattaacaaa
     9721 ctagacgcat agattttatt cttcttttga agcaacccta ttttttactc tttcccattt
     9781 ttgaaaacga tgggcatata gattttcttg atccgttatc ccctcatact cctctataag
     9841 ggcgaagtct gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat
     9901 caatgttcgc cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc
     9961 cgaaacatag atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg
    10021 cattacttcc gcattttctt tcgtgcgaaa atttggctgc gttgtcaaca cgatgatatg
    10081 gcgataagga agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat
    10141 tcgatccatt gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa
    10201 accatttttg ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc
    10261 cgctccttat gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag
    10321 gaattgagaa ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg
    10381 tgggcaatat ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag
    10441 atagttacgt tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc
    10501 tcccattttt acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt
    10561 tttctagatt gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac
    10621 aacggtttta cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt
    10681 tatatagatt atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact
    10741 ttcctgcatc aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa
    10801 atgcattatt agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag
    10861 caaaaaaatg gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa
    10921 atccaagcaa gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag
    10981 atgtgatcgt agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg
    11041 caagcaacca ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa
    11101 acaaagaatc aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt
    11161 attgaacata tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa
    11221 caatggtgtt cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag
    11281 ctgttttttg aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt
    11341 tattgatatt tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg
    11401 tttcatctac aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc
    11461 tttgctcttc tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat
    11521 caaaaaattc ttttgcaaaa tctgcttcga acaatgcttt gacttgttcg tagccgtgtt
    11581 cttctttcaa ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt
    11641 tttccggaag atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca
    11701 ataaaaactt acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta
    11761 ctcggacttc caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg
    11821 gcatccattg atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg
    11881 ttttctcgta atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg
    11941 ttaacacttc tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt
    12001 ttgcaggagc aagtgattca tataaatgga tccgtccatg gaaatttttc atttttccga
    12061 ttttacgtcc taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca
    12121 cacggatgaa ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat
    12181 ccgccccttc tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt
    12241 acaaaggtac gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga
    12301 atgctttgaa cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt
    12361 cggttagttt tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga
    12421 aagaatgatc tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga
    12481 aagaaccaac ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt
    12541 cgattttttc gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc
    12601 aatattgctg aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac
    12661 gatagacatc tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag
    12721 gtttcaacgc ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga
    12781 tttctgaacc aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa
    12841 agtggtctct tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca
    12901 ttccgcggat cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt
    12961 ctgtatctgc atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct
    13021 ggtaattata gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag
    13081 gctgagtacc gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt
    13141 tatcgatata ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat
    13201 taataattgt ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac
    13261 tttcaca
//
LOCUS       DAZKMZ010000065        13244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000065 DAZKMZ010000000
VERSION     DAZKMZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.065"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..541
                     /locus_tag="ACG8M5_002025"
     CDS             <1..541
                     /locus_tag="ACG8M5_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0359269.1"
                     /translation="KHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYH
                     GSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGG
                     PDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYS
                     DKLLNRIDKEILKVGNIDE"
     gene            534..1961
                     /locus_tag="ACG8M5_002026"
     CDS             534..1961
                     /locus_tag="ACG8M5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0359270.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1963..3033
                     /locus_tag="ACG8M5_002027"
     CDS             1963..3033
                     /locus_tag="ACG8M5_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0359271.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3020..4441
                     /locus_tag="ACG8M5_002028"
     CDS             3020..4441
                     /locus_tag="ACG8M5_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0359272.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4454..5461
                     /locus_tag="ACG8M5_002029"
     CDS             4454..5461
                     /locus_tag="ACG8M5_002029"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0359273.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            5473..6477
                     /gene="neuB"
                     /locus_tag="ACG8M5_002030"
     CDS             5473..6477
                     /gene="neuB"
                     /locus_tag="ACG8M5_002030"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0359274.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6474..7622
                     /gene="neuC"
                     /locus_tag="ACG8M5_002031"
     CDS             6474..7622
                     /gene="neuC"
                     /locus_tag="ACG8M5_002031"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0359275.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7595..8272
                     /locus_tag="ACG8M5_002032"
     CDS             7595..8272
                     /locus_tag="ACG8M5_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0359276.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            8223..9404
                     /locus_tag="ACG8M5_002033"
     CDS             8223..9404
                     /locus_tag="ACG8M5_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0359277.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            9417..10394
                     /locus_tag="ACG8M5_002034"
     CDS             9417..10394
                     /locus_tag="ACG8M5_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0359278.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            10394..11302
                     /locus_tag="ACG8M5_002035"
     CDS             10394..11302
                     /locus_tag="ACG8M5_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0359279.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            11303..12055
                     /locus_tag="ACG8M5_002036"
     CDS             11303..12055
                     /locus_tag="ACG8M5_002036"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0359280.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            12060..13007
                     /locus_tag="ACG8M5_002037"
     CDS             12060..13007
                     /locus_tag="ACG8M5_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0359281.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         4761 a         1650 c         2385 g         4448 t
ORIGIN      
        1 aaaacatgat tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc
       61 tataattaat cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt
      121 tgatttgtat catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat
      181 acaaatgtat gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc
      241 agatgaaaaa ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat
      301 tggaggacca gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaacttc
      361 gaaattagca aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga
      421 tatagatcaa ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc
      481 tgataaacta ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata
      541 gaatagtacc tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt
      601 taatcatttg tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga
      661 aaatagcttt tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt
      721 tttttaaaat gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta
      781 taatcattaa cagagaaaat tttttaattc catctattta taattttgga tatttgctga
      841 ttactttgat agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa
      901 aaatggtgta ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta
      961 tctatcaatt tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg
     1021 caagacaagg ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag
     1081 ctatgtttgg atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa
     1141 caaaaacgat tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac
     1201 tttcttcgtc tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt
     1261 tgtttgccaa ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat
     1321 ggatggtaag catattatta gttctattgg tatatttttc ttttggtact aattatgcta
     1381 aggatttttt agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa
     1441 atgaatcaga taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata
     1501 actccgaaat ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac
     1561 caactttgtt atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt
     1621 tgaaaaaagt agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca
     1681 tgcataatgg atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct
     1741 atgctttcta tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga
     1801 attatgcgtt tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg
     1861 tggaggctca tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt
     1921 tgggaagcat ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt
     1981 gcttggactc ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa
     2041 tcaaaaggag atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt
     2101 ataaaagaat tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct
     2161 tcctttaaac gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta
     2221 tccgtttatg atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc
     2281 aatgtttaca aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact
     2341 tatttgaaga agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta
     2401 aataaaaata gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg
     2461 ttactttatg atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg
     2521 atgaaaaaca tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc
     2581 ttttttgatc aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct
     2641 aaagtaaata gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca
     2701 aggacaaatc gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg
     2761 ctacttgaaa atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt
     2821 tcaccatcta tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta
     2881 aatgaatata cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc
     2941 tgtgaacatt ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa
     3001 ttaagaaagt tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa
     3061 gtccagtttc tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat
     3121 tcctattttt gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc
     3181 atttgtatca attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc
     3241 aagttttgac tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac
     3301 actttcgtta attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt
     3361 ttttgatatg aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt
     3421 aattagttat atccagtctt attttacgat taactttaac cattttaaat atttgatttt
     3481 atcttttata tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata
     3541 ttttgatgat aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg
     3601 tttagtaatt tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg
     3661 gagatacgct ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt
     3721 agcacaattc gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata
     3781 ttcctacatt agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc
     3841 ctgggttcca tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt
     3901 tagtaactat tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat
     3961 tgatttagca aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc
     4021 gataacctta ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta
     4081 tgagaaaaaa acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact
     4141 attaaacatg attttcatac ctaggttagg aataattgct gctggataca caaccgtatt
     4201 tacgtatttt ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca
     4261 attatttgac acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat
     4321 acttcatatt ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat
     4381 tagtttagtt atatattttt taaagattaa gaatcgaatt atagatgact ataattcata
     4441 ggaggagata taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat
     4501 tggaagtgaa attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag
     4561 tagaggtgaa gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag
     4621 atttttaatt ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga
     4681 tattattttt catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga
     4741 agctgtaaaa acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa
     4801 tgtaaaacat tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg
     4861 tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac
     4921 agaaacaata ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat
     4981 tcctcttttt agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat
     5041 gacacgattt atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa
     5101 ttcaaaattt ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc
     5161 tcaatcatta ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa
     5221 agttgtaggt atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat
     5281 tgaacgctca tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga
     5341 cgaactgtac aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga
     5401 attaatgagt ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta
     5461 aggagtttta tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat
     5521 ggaagtctag atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg
     5581 gttaaatttc aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa
     5641 tatcagaagg ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa
     5701 ttatctcaat ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt
     5761 ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga
     5821 tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat
     5881 gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta
     5941 gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt
     6001 tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt
     6061 gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct
     6121 aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct
     6181 tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt
     6241 ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga
     6301 aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt
     6361 acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa
     6421 aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa
     6481 aaatacttat agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga
     6541 atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata
     6601 aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc
     6661 aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa
     6721 atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata
     6781 gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata
     6841 ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa
     6901 tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg
     6961 aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata
     7021 gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat
     7081 acgttttgca agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt
     7141 tagctttgat caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata
     7201 gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta
     7261 tttatattac gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta
     7321 tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa
     7381 atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa
     7441 atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct
     7501 tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat
     7561 tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt
     7621 aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat
     7681 tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa
     7741 aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa
     7801 aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat
     7861 aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt
     7921 ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc
     7981 agatgctgta attggagagc atgctataat aaacacaggt agcattgttg agcatgattg
     8041 tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt
     8101 aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc
     8161 ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc
     8221 ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat
     8281 tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata
     8341 attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata
     8401 ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct
     8461 taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag
     8521 ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta
     8581 atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca
     8641 accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac
     8701 gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt
     8761 taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat
     8821 ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa
     8881 aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc
     8941 aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg
     9001 gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata
     9061 aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc
     9121 caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta
     9181 acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac
     9241 atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt
     9301 cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg
     9361 aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga
     9421 acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac
     9481 actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg
     9541 aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa
     9601 taaattcagt ttttgtttgt acatccccta taacccatga atttattata aaagaggctt
     9661 taaatttaga ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa
     9721 taatcgaagt agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga
     9781 gaaaagaaat tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat
     9841 accatgtagg acagtattta cccgactggc atccttggga agattatcgc aatttttttg
     9901 tttcaaacaa aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta
     9961 tagatacatt tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg
    10021 atattgatta ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa
    10081 ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa
    10141 agattttaat agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt
    10201 ctagtatgac agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata
    10261 ctattattga agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga
    10321 aaatggaacc ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca
    10381 ttgaggtaaa ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca
    10441 tctaaaaaat ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta
    10501 tagtagcagt ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt
    10561 ggatacacat tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact
    10621 aaatgtaatt gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag
    10681 tattgatctt actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag
    10741 gtataaaaat atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt
    10801 aagagctatt tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa
    10861 ttattctgcc tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga
    10921 ttatattata aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc
    10981 gaatcttgaa attaacagta atgatttagc aacatatata ttgagatacg atgataaaat
    11041 tatagaaatt catttagatt attttggaag agttcctaca agaaaatttc aattgtttac
    11101 tttagatgaa tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat
    11161 tgttaaagaa atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt
    11221 tgaaaacgta gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt
    11281 gaaaatagct gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc
    11341 taaggggttt aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc
    11401 tttagcaatg atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt
    11461 gaatacggac agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat
    11521 tgtagaacgt aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga
    11581 ttcattctta caactagaaa gagaagataa tatatttgat ttaattatag atttagattt
    11641 gacttcacct ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa
    11701 tttagataaa gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat
    11761 ggtaaaaaga attgaaaatg aagttactat agttaataaa tcaagataca catctaggca
    11821 acagacacct gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct
    11881 aaaaaaaagt aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct
    11941 aattttagat attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt
    12001 tttagaagat tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga
    12061 tgagctgtta tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt
    12121 taggagaaga acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc
    12181 ttgatatgac gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg
    12241 aagagatcat gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg
    12301 tggcagattc agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa
    12361 tgattttaga attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt
    12421 cagcggctgt ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc
    12481 cattgacgcc ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc
    12541 atttatatga tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa
    12601 atccgaattc tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa
    12661 tcgtaggaga agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg
    12721 tttatataga tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg
    12781 ggaaacaatt caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa
    12841 tcaatgaaat tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata
    12901 tccatgactc tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg
    12961 gtattttaag tggaatgact aattatttga aaactgaaat caattaaaac atactaagat
    13021 aagattagag ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca
    13081 aatctgggat agaagggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat
    13141 ttggacagaa cagaaagaag aaaacctctt tctgttctgt ttatttggtt aaaaatgttt
    13201 tgccatgacg aatcataatt cgtagtttga aattgtaaaa gttg
//
LOCUS       DAZKMZ010000066        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000066 DAZKMZ010000000
VERSION     DAZKMZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.066"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8M5_002038"
     CDS             147..1016
                     /locus_tag="ACG8M5_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0359282.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8M5_002039"
     CDS             1225..2250
                     /locus_tag="ACG8M5_002039"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0359283.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8M5_002040"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8M5_002040"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0359284.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8M5_002041"
     CDS             3333..5036
                     /locus_tag="ACG8M5_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359285.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8M5_002042"
     CDS             5038..7071
                     /locus_tag="ACG8M5_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0359286.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8M5_002043"
     CDS             7082..7522
                     /locus_tag="ACG8M5_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359287.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8M5_002044"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8M5_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359288.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8M5_002045"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8M5_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0359289.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG8M5_002046"
     CDS             9793..11559
                     /locus_tag="ACG8M5_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0359290.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG8M5_002047"
     CDS             11574..12011
                     /locus_tag="ACG8M5_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359291.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG8M5_002048"
     CDS             12024..13175
                     /locus_tag="ACG8M5_002048"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0359292.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKMZ010000067        13142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000067 DAZKMZ010000000
VERSION     DAZKMZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.067"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8M5_002049"
     CDS             complement(24..722)
                     /locus_tag="ACG8M5_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0359293.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8M5_002050"
     CDS             complement(715..2067)
                     /locus_tag="ACG8M5_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0359294.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8M5_002051"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8M5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0359295.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8M5_002052"
     CDS             3851..4588
                     /locus_tag="ACG8M5_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0359296.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8M5_002053"
     CDS             4713..5192
                     /locus_tag="ACG8M5_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0359297.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8M5_002054"
     CDS             5189..5956
                     /locus_tag="ACG8M5_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0359298.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8M5_002055"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8M5_002055"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0359299.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8M5_002056"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8M5_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0359300.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8M5_002057"
     CDS             7567..7797
                     /locus_tag="ACG8M5_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359301.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8M5_002058"
     CDS             7788..8036
                     /locus_tag="ACG8M5_002058"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0359302.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8M5_002059"
     CDS             8142..8726
                     /locus_tag="ACG8M5_002059"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0359303.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8M5_002060"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8M5_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0359304.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8M5_002061"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8M5_002061"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0359305.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8M5_002062"
     CDS             10627..10806
                     /locus_tag="ACG8M5_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359306.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8M5_002063"
     CDS             10828..11853
                     /locus_tag="ACG8M5_002063"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0359307.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..>13142
                     /gene="glyA"
                     /locus_tag="ACG8M5_002064"
     CDS             12028..>13142
                     /gene="glyA"
                     /locus_tag="ACG8M5_002064"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0359308.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFK"
BASE COUNT         4494 a         2369 c         2652 g         3627 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 ga
//
LOCUS       DAZKMZ010000068        12773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000068 DAZKMZ010000000
VERSION     DAZKMZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.068"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..853
                     /locus_tag="ACG8M5_002065"
     CDS             284..853
                     /locus_tag="ACG8M5_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0359309.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            840..1700
                     /locus_tag="ACG8M5_002066"
     CDS             840..1700
                     /locus_tag="ACG8M5_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0359310.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1890..2594
                     /gene="yycF"
                     /locus_tag="ACG8M5_002067"
     CDS             1890..2594
                     /gene="yycF"
                     /locus_tag="ACG8M5_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0359311.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2599..4434
                     /gene="walK"
                     /locus_tag="ACG8M5_002068"
     CDS             2599..4434
                     /gene="walK"
                     /locus_tag="ACG8M5_002068"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0359312.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4431..5747
                     /gene="yycH"
                     /locus_tag="ACG8M5_002069"
     CDS             4431..5747
                     /gene="yycH"
                     /locus_tag="ACG8M5_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0359313.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5748..6617
                     /locus_tag="ACG8M5_002070"
     CDS             5748..6617
                     /locus_tag="ACG8M5_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0359314.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6672..7481
                     /locus_tag="ACG8M5_002071"
     CDS             6672..7481
                     /locus_tag="ACG8M5_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0359315.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7584..8213)
                     /locus_tag="ACG8M5_002072"
     CDS             complement(7584..8213)
                     /locus_tag="ACG8M5_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0359316.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8383..9675
                     /locus_tag="ACG8M5_002073"
     CDS             8383..9675
                     /locus_tag="ACG8M5_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359317.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9751..10413)
                     /locus_tag="ACG8M5_002074"
     CDS             complement(9751..10413)
                     /locus_tag="ACG8M5_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0359318.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10638..10922
                     /gene="groES"
                     /locus_tag="ACG8M5_002075"
     CDS             10638..10922
                     /gene="groES"
                     /locus_tag="ACG8M5_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0359319.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10973..12598
                     /gene="groL"
                     /locus_tag="ACG8M5_002076"
     CDS             10973..12598
                     /gene="groL"
                     /locus_tag="ACG8M5_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0359320.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4246 a         2201 c         2765 g         3561 t
ORIGIN      
        1 cattcataga ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct
       61 tgcaagagaa caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg
      121 gcgggtctcc tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt
      181 tcatggaatc ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata
      241 agtctgatat catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca
      301 gcaattaatg gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact
      361 ccctgactta gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc
      421 tgtgatacaa ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa
      481 actaggtttg ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt
      541 acttgcaatc actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt
      601 gttccaagga aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga
      661 agcagctgtg tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga
      721 ccaggcaacc cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa
      781 acttcttgcg gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa
      841 tgaaaaacaa taaaatcgca attttagtag attcaggtac ggatgttcca gaagaattga
      901 taaaaaaata cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata
      961 cagataaaat tgacattacc cctgaagaga tctatcaacg tttgccagag gaaatccctg
     1021 gtacatcttt gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg
     1081 gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg
     1141 ttcgtttaat tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata
     1201 ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag
     1261 aatgggatca actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta
     1321 atgttgcgac attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg
     1381 taggaaccgc tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca
     1441 cagtaggaaa agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag
     1501 aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag
     1561 aagcaattga aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact
     1621 ttggccagat ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg
     1681 tacaagtttt agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc
     1741 cttttttatt gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt
     1801 ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta
     1861 tgacaataat atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga
     1921 agccgatttc ggatatcgtc aaattcaatt tagccaaaga aggctatgac gtttatactg
     1981 catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac
     2041 ttgatttgat gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt
     2101 atgatatgcc gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct
     2161 tagagttagg cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac
     2221 gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac
     2281 ctgcggaatt gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac
     2341 gaggagaaac aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc
     2401 accttggaca agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt
     2461 ttggagatgt acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata
     2521 atccaagcca tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc
     2581 cagaacagga gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag
     2641 atcgctatta cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt
     2701 atacgtggtt tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg
     2761 gaatcattag caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa
     2821 gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa
     2881 atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc
     2941 gctgtgggaa agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt
     3001 gctttagata acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc
     3061 ggcgatgccg ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa
     3121 atcaataaca cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg
     3181 atcgtatctg ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa
     3241 gccattcgaa tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa
     3301 cttggacagc tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat
     3361 acaatggaag ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc
     3421 attgcgacag atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta
     3481 gatatcaaaa acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa
     3541 gaatatacgt tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc
     3601 agtacattgg aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa
     3661 tcaggtttta tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca
     3721 gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca
     3781 agtatgcgta gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca
     3841 ccaaattttt tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac
     3901 ctcttaaatc tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat
     3961 ttcaacgagt tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa
     4021 aaaaattata agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca
     4081 gataaaatca tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat
     4141 gggggaacga ttacttgtcg cttattagaa acgcataata acgtgatttt aagtatcacc
     4201 gatcaaggat tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga
     4261 gtagataaag cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga
     4321 gaagtgatta aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg
     4381 actttttata tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct
     4441 ctgaaaaagt cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt
     4501 atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc
     4561 aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta
     4621 catggcacca ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc
     4681 aattgatcat cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat
     4741 cacagtttga acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt
     4801 ttttgctaag agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa
     4861 aagaaagcga aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc
     4921 gatttttgaa ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag
     4981 atatcaataa agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat
     5041 cgacgatcga cacgcaatat gctacgaaac attcgatcaa actgaagaag tatagttata
     5101 tcctgtcaca gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg
     5161 tgaaaaataa tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta
     5221 tccattcgga aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg
     5281 ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc
     5341 tcagatattt tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc
     5401 cggtttttgg gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg
     5461 gttctgtgca agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag
     5521 acgaagaagt agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag
     5581 cagatagcaa caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa
     5641 cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt
     5701 cagctaacga ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa
     5761 aaatcgaatg gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt
     5821 atcatgacgc ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc
     5881 aacaacgtct ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc
     5941 aaggctatta tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca
     6001 aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga
     6061 aatctttatc cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt
     6121 ccttggaaga ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca
     6181 cgaaaaactt ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg
     6241 aaggcatccc attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata
     6301 atgattatag catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg
     6361 aaaagacgga actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa
     6421 taccaagagg atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc
     6481 gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg
     6541 tccaaattga gagcgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa
     6601 aagtggaaga tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa
     6661 gtaggagtca aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa
     6721 gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac
     6781 ttagcggtaa aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag
     6841 atggaatact tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc
     6901 gtaagtatca tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga
     6961 ttggaaatgt accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg
     7021 gcgatatcga tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc
     7081 gtttccataa agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt
     7141 atatgcgagg tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg
     7201 agatgttacg catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag
     7261 gacatctttc caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca
     7321 aacgaatcta tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa
     7381 caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg
     7441 atacagatcc agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa
     7501 tataaaaagc agaagataaa atcaatcagg gttggtttta tcttctattt ttattttttt
     7561 agcatattaa aaaaataacc gtattatcga ctgactacac tgatgtgctg cttttcatgt
     7621 ttctcatcca gtatctcatc tactaatgca atcgcgtaat ctgccatgct gatcgtactg
     7681 tcgctagcag cgtttctttg taaataatca tcacttaaca aataagtacc tgtacgtttg
     7741 ccatgtggat caaataaaga agctggactg atataagtcc aatgtagatt tttagattgt
     7801 ttcaatttta acaatccttc gtacatattg aaagctgttg gatagaaggg tgcattttta
     7861 ggtacttggt caatcatacg agtcgtcttt tctgtatcta agaataacga agaggcaccc
     7921 ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct caatcaaatg ggacaaagaa
     7981 gtttgatgca tttcttcttt tcctttggga gcgttgaaag catcaacgac aacatcaaaa
     8041 gatgcaagat cagtagttgt caactcaaat aaatcctttt gtataaacgg aacatctgat
     8101 ggcaattttt ctggatgacg cacgattgcc gtcacgtcta aatctctgga caatgcttcg
     8161 tctaaaataa atgaacctgc atggccagta gctccaatga gtgcaatttt cataaaaaac
     8221 ctcctttttt attcctgtcc attgtaagca gttgctgaat attcgtcaaa gaatccgatt
     8281 tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat cattgggtat agttaaatta
     8341 agaaaactga ttgataaaaa tagaagttga aggagataag aaatgaaaga gtggttagat
     8401 cgtgcctttt taccaatgat aacatctgtt gtgaatctta gacccttaac tgctttgcgt
     8461 aatggaatga cttacacgat cccaataatt attgttggct cgctgttttt cttatttgct
     8521 cattttcctg tggattacat cgctcagtgg atagacaaaa caggattttc tatgtatttt
     8581 acacaagtat accaggctac atttgatgtg atagcctttt ggatcgtttt tgccattgca
     8641 tacgcttatg caaaagaaga agacatcgat cgcttaccag tcggcataac agccttgtct
     8701 gcttatctac tgcttattca gccactaaat actcagatag atactgtctg gacaggtgct
     8761 gaagggatac taggagcagt tacaactggg ctcatcatcg gatggggcta tgcacgttta
     8821 gtaagacttg cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct
     8881 tttacttcat tgattccaat cattgttata gtcactttta cgcttatcgg ttcagcggtt
     8941 tttcaacatt tgttcagaat gactgttgtt caaggagtat ggacatatgt ccagcttccc
     9001 attcaatcgt tcatagacac gttgtttggt gtgcttttat taggtttttt tgttccgttt
     9061 ttatggttat ttggtcttca tggatctgcg atggtcaatg ggttggttag tcccgtcttg
     9121 caagcgaatt cattagcaaa tgcagaaatc ttagcctcag gacaagagtt gacggtggca
     9181 aatggcggac atatcgttac tcagcaattt ctcgatcagt tcatgacggt tacgggtgct
     9241 ggattgacgt taggggctgt attttttatg acggtttttg cgaaatcgaa gaaatacaga
     9301 gaattaggaa atcttgctct tttaccagcc tttttcaaca tcaatgaatc aattattttt
     9361 tccacaccga tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc accggtcgtt
     9421 tcaggattga ttacctattc cgctttatat tttggattcg ttcctttatt tacagcagtc
     9481 caagtccctt ggacaacacc gccgatcctc tcgggtttct tagtaggcgg agtgcctgca
     9541 gcgattttac aaggtatcgt gttgtcaatt agttttttca tttatttccc atttttgaaa
     9601 aaaatagatt ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg agcagcagaa
     9661 aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt agattgggaa aatcaccaat
     9721 ctaggatgac aggagtattt atagtcatgt ttatactagc cctttttgga cagctaattg
     9781 aacaagaaca actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc agatccgacc
     9841 agttgctttg tactctaggc tgaagaagaa acctaggaaa aagtagatta gcagatgacc
     9901 atcattatga gcaaacgcaa acagcaaaga gctgatcact gctccgaccc aaaaatttga
     9961 atatcgactg atgatcccca gtatactgcg gcggaatacg aactcttcca tgatgggtcc
    10021 agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg atcatctgga tgatattttg
    10081 tgtattctcg gaaggggttg ctttgccaaa caatacagat tctaacatga cagcgatgct
    10141 ttgcagaatc aatgcgacaa agattccaac caatccataa ataacaatct ttggagcaga
    10201 agcttttttt accgctgatt cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc
    10261 taggacaacg gcgcctagta tatacatgac tgcagttgct gtgatcaatt cagacgtaga
    10321 ttttacaagt cctatcgaag ctaggatcaa gggtgagata aaaattagac catagcaaaa
    10381 aatgctgagg aagctatatt tttttaaaga catgtaattc ctccttttaa gggataacaa
    10441 gcatgcaagg caagcttata tcgattgatt tcaattatat cataatttca ataagcagct
    10501 gtcagaataa aatttctttt ctttgattta aaagcttgca aaaaagaggg agaatgagta
    10561 ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc taacagatta aaaaaagagt
    10621 tatcggaggg atttatcgtg ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa
    10681 aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc
    10741 aaactgggac agttgttgca gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc
    10801 ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt acagaagtaa
    10861 aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg attgttgagt
    10921 aaatttggaa gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa
    10981 agaattgaaa tttgcagaag acgcacgcgc agctatgtta cgcggggtag ataaattagc
    11041 agatacagtg aaagtaacat taggacctaa aggccgaaat gtcgtactag aaaaatctta
    11101 cggctctcca ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg aattagaaga
    11161 tcattttgaa aacatgggtg caaaacttgt atcagaagta gcatcaaaaa caaatgatat
    11221 tgcaggagac ggtacaacga cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt
    11281 aaaaaacgta actgcaggtg ctaatcctct aggcattcgt cgcgggatcg aattggcaac
    11341 aaaagcagca gtcgaagaat tgcataatat ctcaactgtt gttgattcaa aagaagccat
    11401 tgcacaagta gcagctgttt cttcgggttc tgataaagtt ggacatttga ttgctgatgc
    11461 aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac
    11521 agaactagat gttgtagaag gtatgcagtt tgatcgtggt tacttatcac aatatatggt
    11581 aacagataac gacaaaatgg aagctgttct agaaaaccca tatatcttga tcacagacaa
    11641 aaaaatctct aacatccaag atattctgcc attgttagaa caaatcttgc aacaatcacg
    11701 tccattgttg attattgctg acgatgtaga tggtgaagca ttgccaactc ttgtattgaa
    11761 caaaatccgt ggaacattca acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg
    11821 taaagcaatg cttgaagata tcgctatttt gacaggtggt acagtaatta ctgacgatct
    11881 aggtttagaa ttgaaagatg caacaatcga aaacttagga aatgcttcta aagttgttgt
    11941 agataaagac aatacaacga tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg
    12001 tgtgcaattg atcaaaaacc aaattgctga aacaacttct gattttgacc gcgaaaaatt
    12061 acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac
    12121 tgaaacagaa ttgaaagaat taaaattacg cattgaagat gcattgaatg ctacacgtgc
    12181 cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa
    12241 agtttctgct gtagaagcag aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc
    12301 tttagaagaa ccaattcgtc aaatcgctga aaacgctggt tatgaaggat cagtgattgt
    12361 tgataaattg aagaacgtgg aactaggcac tggtttcaat gctgctactg gagaatgggt
    12421 aaacatggtt gaagcaggta tcgtagaccc aactaaagta acacgttctg ccttgcaaaa
    12481 tgccgcttcc gtttctgcct tgttattaac gacagaagca gtcgttgcag ataaaccaga
    12541 accagcagcg ccagcagctc ctgcaatgga tccatcaatg atgggcggta tgatgtaaga
    12601 aaaagcaact tgcttatcta caactgatca ttagtgaaag acagataaaa agaggctgga
    12661 acagaagcgt ttaactccaa gaaataagaa gaaattcacg gaaattgctt ttcaaatttt
    12721 tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgctgttta tac
//
LOCUS       DAZKMZ010000069        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000069 DAZKMZ010000000
VERSION     DAZKMZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.069"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M5_002077"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M5_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0359321.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8M5_002078"
     CDS             complement(323..892)
                     /locus_tag="ACG8M5_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0359322.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8M5_002079"
     CDS             1078..1980
                     /locus_tag="ACG8M5_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0359323.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M5_002080"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M5_002080"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0359324.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M5_002081"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M5_002081"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0359325.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M5_002082"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M5_002082"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0359326.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8M5_002083"
     CDS             8554..8739
                     /locus_tag="ACG8M5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0359327.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8M5_002084"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8M5_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0359328.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8M5_002085"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8M5_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0359329.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8M5_002086"
     CDS             11896..12705
                     /locus_tag="ACG8M5_002086"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0359330.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKMZ010000070        12743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000070 DAZKMZ010000000
VERSION     DAZKMZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.070"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..545
                     /locus_tag="ACG8M5_002087"
     CDS             102..545
                     /locus_tag="ACG8M5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0359331.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(551..1735)
                     /locus_tag="ACG8M5_002088"
     CDS             complement(551..1735)
                     /locus_tag="ACG8M5_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0359332.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2003..2701
                     /locus_tag="ACG8M5_002089"
     CDS             2003..2701
                     /locus_tag="ACG8M5_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0359333.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2708..3385
                     /gene="nth"
                     /locus_tag="ACG8M5_002090"
     CDS             2708..3385
                     /gene="nth"
                     /locus_tag="ACG8M5_002090"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0359334.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3511..4044)
                     /locus_tag="ACG8M5_002091"
     CDS             complement(3511..4044)
                     /locus_tag="ACG8M5_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359335.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4341..6722)
                     /locus_tag="ACG8M5_002092"
     CDS             complement(4341..6722)
                     /locus_tag="ACG8M5_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0359336.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6712..7359)
                     /gene="recU"
                     /locus_tag="ACG8M5_002093"
     CDS             complement(6712..7359)
                     /gene="recU"
                     /locus_tag="ACG8M5_002093"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0359337.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7423..7962
                     /locus_tag="ACG8M5_002094"
     CDS             7423..7962
                     /locus_tag="ACG8M5_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0359338.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8052..8471
                     /gene="gpsB"
                     /locus_tag="ACG8M5_002095"
     CDS             8052..8471
                     /gene="gpsB"
                     /locus_tag="ACG8M5_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0359339.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8493..8860
                     /gene="rnpB"
                     /locus_tag="ACG8M5_002096"
     ncRNA           8493..8860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8M5_002096"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9069..10235
                     /locus_tag="ACG8M5_002097"
     CDS             9069..10235
                     /locus_tag="ACG8M5_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0359340.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10281..11777
                     /locus_tag="ACG8M5_002098"
     CDS             10281..11777
                     /locus_tag="ACG8M5_002098"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0359341.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11858..12541
                     /locus_tag="ACG8M5_002099"
     CDS             11858..12541
                     /locus_tag="ACG8M5_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0359342.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSETAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12550..>12743
                     /locus_tag="ACG8M5_002100"
     CDS             12550..>12743
                     /locus_tag="ACG8M5_002100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359343.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4274 a         2230 c         2629 g         3610 t
ORIGIN      
        1 ttttttcttg gtttcagatt gggttttcct acaaaatatt tttatctttg ttgaatagga
       61 aacaaaaaaa caatataatg aaagaaaaag aaggtgtaga aatggataaa agacaactgc
      121 taatagaaga atatctggat ttagctaatg agattattca tcgtaataaa ccagaaatgg
      181 aaaaagaatt tgcaggattt actctaaatg aactagaagt gattgaacat atcgggaaaa
      241 tccctgatcc taacgtgaca aaattagcga atgctagtta catgactaga ggagccatca
      301 gtaagctaac aaaaaaactg atagctagag ggatcataaa cacttatcaa agcgaagaga
      361 ataaaaaaga aatctatttt caattgacgc caatcggaca aaaaataagg aatagacacg
      421 agcaactcca tcaaaaatgg gtacagcgag atgctactgt atttgaaaat atgtcaaaag
      481 aagaatttga cacagtattt cgttttatcg gtcgatacag agaatttata cgaagtagca
      541 aatgaagcta ctatttttta ttgtagtgat aataggcaaa aatcataagc aaaaatccga
      601 ataccgtcgg aagaaatggt gctgcagcat aaagctgtcc tgcgattagc gggccgatga
      661 cgcgcgatag agattgtata ctttgggtac cgcccattac aagaccttgc tggttatctg
      721 agacagaatt ggatagttgg ccattgaata caggagtaaa aatagaatca ccgaaaccat
      781 aacagataat cccaaataaa aacaaaatcg gcagtagaag aattccagat aatgtgatga
      841 atagataggc gaggatttca ctgaacatcc ctattcttgt gatctgctgt tctgaaaatt
      901 ttttgagcaa tcgaggcata atgaacaatt gcgagacagt atccaagatt ccaatcaaag
      961 agaagcttaa accaaccaac ccagccttcc actgaaaggc gtcaatcgaa aattgagaaa
     1021 agatagattg aagagagcca gctgccaacc aaacagcaaa acctgctgtc aataaaggaa
     1081 taacggatga aatcttgaat aattgttgta attggtgaaa aggtcttaca tgggagaaac
     1141 ttaaatctac tgaacgtttt tttattggaa ggctttctag cataaatgtg tagccataaa
     1201 cggcatttaa gaaagtaaag agtgctccaa taaatatagg aacagaattc cccaactcag
     1261 ctaatagacc ccctataata ggacccagcg ttgttcctat accgactaaa gcacccatcc
     1321 atccaaaaac tttggttctt tcattaggtt ccgtgatgtc ggcaaagtaa gcatataagg
     1381 tggagatttc tcctgccgtc aatccttcaa tgatacgacc aaggaacaac atccatatcg
     1441 aatttcctaa accaaaaagc aaatatccga tacttgatcc taataaactg ataataagga
     1501 tcggatgacg cccaaaatga tcacttaagc taccgagaat gggggcagat aagaaggcag
     1561 ctagagcata aacagacatg agcaaagtga tatagaaagc ttgctgatgt atggagtgag
     1621 aataaggcag cgttaaaaac ggaagtacag gactgacgat agtaaatcct aatccagtga
     1681 ataagacaga gacgtatcca aaaactttag aatgtttatt tgtgaaactg tacatgtaaa
     1741 aactccttta tgcaataatg ttaacttgga aacaaaaata gtgtatagta attttgtttc
     1801 cttgtcaaca ttctaaggaa cagcgaaatc tacttaattt atttattccg aaaagtgaag
     1861 atacgcatca aaaacatgaa caaacaaaaa tcaagtagac gacattgtaa atctgaccga
     1921 tcagtgaagt aaatagaaaa gaaacctgtt cttgcttccc atgttttttt tacgtatact
     1981 gtgattgagc ggaggagaaa tcatgttgaa tttagaaaat tacttaagcg ctggacaaac
     2041 agtcatatct aatcttttac ttaaaaatta tcataggatc ggtctacaat ctgaagaatt
     2101 tttatttgtt ctacagttgc atatggcaca gcttgaagga gatactttcc ctgatttgca
     2161 gatgattgct atggatatgg gaatcaaaca agatcagatt tttcaaatac tggatcgtct
     2221 cgtaacaaat ggatttatca agatagaaac gacgattgtc aacggtaaaa aagcagatcg
     2281 atataatctt taccccatct atgatctatt aggtgagtat ttaaaaacac aagaaaaaaa
     2341 atacgaagaa aaaacacaag aaaaagaaat ccagtcagtg tatcaattat ttgaacaaga
     2401 atttggacgc ccgctttctt cgatcgagtt ccagcggatc ggacaatggc tagaagaaga
     2461 ccattatcag ccggaaattt tgaagctagc actgcgggaa gccgtattaa atcaggctta
     2521 tagttttaat tacgttgacc gcattttgct atcgtgggaa agaaaaaatc tacgtacaaa
     2581 acaacaagtg gaagaagacc aaaaacgaag aaaacagcag cttcttcaaa gagaagtaga
     2641 acagtcagct caacaagaag aattgccgaa agtctcactg aaaaattggt tggaggagta
     2701 gcggaatatg ttaagcaaac aaaaaacaat ggaagctcta gaaacgatgt atggaatgtt
     2761 tcccgaagca catggagaac tcaagcacaa taatccattt gagttattga ttgcagtgat
     2821 cttaagtgcc caggcgacag atgtatcagt gaataaagca acgccagatt tgtttgcctc
     2881 ttttccgaca cccgatgcgt tagcagaagc ttcaatcgat gaaatcattc ttaaaataaa
     2941 aacgattggt ttgtatcgaa ataaagcaaa aaacatcaaa gcgtgtgcac agcagctgat
     3001 tgaacgtttc gatgggcaag tgccaacgtc aagagaagaa ttgatgtctc ttcctggtgt
     3061 aggcagaaag acagcaaatg tcgtattggg ggatgcattc gggataccag caatcgcagt
     3121 agatacccac gtggaacgtg tctcaaaacg cctgaggatc tgcaaactag atgctacggt
     3181 catggaagta gaagaaacac tgatgcggaa agtaccacaa gaattatggg taaagacaca
     3241 tcatacactg attttttttg gtagatatca ttgcacggca agaaatccca agtgcgaggt
     3301 atgtcctttg ctatcaatat gtcaagatgg gaaaaatcgg atgcgtctaa aagaaaaggc
     3361 gctaaaaaag aaaccacgcc tgtagacgtg gtttctttaa ttgcaaataa atgtatgaat
     3421 tatcttgtta ttatggagaa aagctgtttc gtttatcttt tttgcatgaa aaagtaaatg
     3481 tacatactca gtagctagga tggtaactta ttacaattta ttgcgataaa aataaaagtt
     3541 gttattttta ttgggaaaat acccgatgta gaaaataatg ccttccaata tacctataaa
     3601 ataagtccag tattccaaag gattatataa ataagtgacg tatctatcta aaggtgtaat
     3661 aaaaatgata agtgataata aaaaaatacc atttataact aaacataaac taatggattt
     3721 gggcggtttc aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt
     3781 gagaatcatt gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag
     3841 tacagaaaaa ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa
     3901 acaaaatttt tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag
     3961 atggtgcaga caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc
     4021 tttcaaaaaa ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt
     4081 attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa
     4141 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
     4201 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
     4261 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
     4321 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
     4381 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
     4441 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
     4501 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
     4561 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
     4621 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
     4681 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
     4741 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
     4801 gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
     4861 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
     4921 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
     4981 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
     5041 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
     5101 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     5161 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     5221 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     5281 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     5341 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     5401 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     5461 ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta
     5521 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     5581 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     5641 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     5701 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     5761 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     5821 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     5881 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     5941 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     6001 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     6061 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     6121 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     6181 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     6241 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     6301 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     6361 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     6421 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     6481 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     6541 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     6601 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     6661 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     6721 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     6781 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     6841 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     6901 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     6961 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     7021 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     7081 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     7141 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     7201 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     7261 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     7321 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     7381 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     7441 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     7501 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     7561 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     7621 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     7681 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     7741 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     7801 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     7861 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     7921 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     7981 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     8041 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     8101 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     8161 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     8221 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     8281 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     8341 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     8401 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     8461 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     8521 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     8581 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     8641 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     8701 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     8761 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     8821 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     8881 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     8941 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     9001 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     9061 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     9121 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     9181 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     9241 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     9301 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     9361 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     9421 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     9481 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     9541 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     9601 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     9661 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     9721 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     9781 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     9841 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     9901 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     9961 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
    10021 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
    10081 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
    10141 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
    10201 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
    10261 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
    10321 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
    10381 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
    10441 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
    10501 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
    10561 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
    10621 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
    10681 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
    10741 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
    10801 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
    10861 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
    10921 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
    10981 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
    11041 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
    11101 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
    11161 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
    11221 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
    11281 gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
    11341 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
    11401 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
    11461 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
    11521 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
    11581 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
    11641 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
    11701 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
    11761 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
    11821 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
    11881 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
    11941 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
    12001 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
    12061 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
    12121 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaaacggc
    12181 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
    12241 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
    12301 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
    12361 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
    12421 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
    12481 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
    12541 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
    12601 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
    12661 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
    12721 ttggcaggag aacaccgttt att
//
LOCUS       DAZKMZ010000071        12709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000071 DAZKMZ010000000
VERSION     DAZKMZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.071"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG8M5_002101"
     CDS             <1..730
                     /locus_tag="ACG8M5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0359344.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG8M5_002102"
     CDS             788..2827
                     /locus_tag="ACG8M5_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0359345.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG8M5_002103"
     CDS             complement(2936..4087)
                     /locus_tag="ACG8M5_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0359346.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG8M5_002104"
     CDS             complement(4241..4771)
                     /locus_tag="ACG8M5_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0359347.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG8M5_002105"
     CDS             complement(4870..5220)
                     /locus_tag="ACG8M5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0359348.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8M5_002106"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG8M5_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0359349.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8M5_002107"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG8M5_002107"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0359350.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG8M5_002108"
     CDS             complement(7623..8285)
                     /locus_tag="ACG8M5_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359351.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG8M5_002109"
     CDS             8469..10115
                     /locus_tag="ACG8M5_002109"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0359352.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002110"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002110"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0359353.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..>12709)
                     /locus_tag="ACG8M5_002111"
     CDS             complement(11296..>12709)
                     /locus_tag="ACG8M5_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359354.1"
                     /translation="NFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYY
                     RLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLS
                     ISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVL
                     DRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPIC
                     REQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIP
                     EKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFRE
                     QNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQI
                     FSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYI
                     FELKEEALMDQSKKENQVTS"
BASE COUNT         4251 a         2345 c         2356 g         3757 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaattt
//
LOCUS       DAZKMZ010000072        12520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000072 DAZKMZ010000000
VERSION     DAZKMZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.072"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..160)
                     /locus_tag="ACG8M5_002112"
     CDS             complement(<1..160)
                     /locus_tag="ACG8M5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0359355.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEIS"
     gene            complement(157..810)
                     /locus_tag="ACG8M5_002113"
     CDS             complement(157..810)
                     /locus_tag="ACG8M5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0359356.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1098..1618
                     /locus_tag="ACG8M5_002114"
                     /pseudo
     CDS             1098..1618
                     /locus_tag="ACG8M5_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1616..2335)
                     /locus_tag="ACG8M5_002115"
                     /pseudo
     CDS             complement(1616..2335)
                     /locus_tag="ACG8M5_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2286..2495)
                     /locus_tag="ACG8M5_002116"
     CDS             complement(2286..2495)
                     /locus_tag="ACG8M5_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359357.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2477..2545)
                     /locus_tag="ACG8M5_002117"
                     /pseudo
     CDS             complement(2477..2545)
                     /locus_tag="ACG8M5_002117"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            2772..4045
                     /locus_tag="ACG8M5_002118"
                     /pseudo
     CDS             2772..4045
                     /locus_tag="ACG8M5_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4152..5183)
                     /locus_tag="ACG8M5_002119"
     CDS             complement(4152..5183)
                     /locus_tag="ACG8M5_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359358.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5190..6020)
                     /locus_tag="ACG8M5_002120"
     CDS             complement(5190..6020)
                     /locus_tag="ACG8M5_002120"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359359.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6017..6832)
                     /locus_tag="ACG8M5_002121"
     CDS             complement(6017..6832)
                     /locus_tag="ACG8M5_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359360.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6829..7653)
                     /locus_tag="ACG8M5_002122"
     CDS             complement(6829..7653)
                     /locus_tag="ACG8M5_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0359361.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7643..8743)
                     /locus_tag="ACG8M5_002123"
     CDS             complement(7643..8743)
                     /locus_tag="ACG8M5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0359362.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8921..9706
                     /locus_tag="ACG8M5_002124"
     CDS             8921..9706
                     /locus_tag="ACG8M5_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0359363.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9739..10122
                     /locus_tag="ACG8M5_002125"
     CDS             9739..10122
                     /locus_tag="ACG8M5_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0359364.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10198..10329
                     /locus_tag="ACG8M5_002126"
     CDS             10198..10329
                     /locus_tag="ACG8M5_002126"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0359365.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10298..10534
                     /locus_tag="ACG8M5_002127"
     CDS             10298..10534
                     /locus_tag="ACG8M5_002127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0359366.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10546..10632
                     /locus_tag="ACG8M5_002128"
                     /pseudo
     CDS             10546..10632
                     /locus_tag="ACG8M5_002128"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10612..11583)
                     /locus_tag="ACG8M5_002129"
     CDS             complement(10612..11583)
                     /locus_tag="ACG8M5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0359367.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12235..>12520
                     /locus_tag="ACG8M5_002130"
     CDS             12235..>12520
                     /locus_tag="ACG8M5_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0359368.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3756 a         2819 c         2293 g         3652 t
ORIGIN      
        1 tagaaatttc atttgccata ttatgaatct tttcttttct cgtctccaaa tatgttttag
       61 gaaagataaa aaaaattaat gaatgaacta atataattat tattccaaga acagaaaata
      121 tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca atttatatcc
      181 tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt cttttatata
      241 tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat aatcaataat
      301 ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata cttctagttc
      361 ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat taaaattaac
      421 cgatagatcc ttatattcaa acttctctaa atgtccataa ttcttcttaa ttaaagaatc
      481 tattctagct tttaagaccg gcaatgaaaa tggcttttct acatatccgt ctactaaatt
      541 agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta gaattggtaa
      601 attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag gtatctgaat
      661 gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc ttccatcttt
      721 agcttgaata acagtatatc caaattctga aagatattca cttattccct ctcttatcat
      781 attatcatct tcaacagtta atattttcat atatacctcc acttaagcct atacactaga
      841 tattatacag catattatgg ctctataata tctagtgttg gtgacgcctt ctgttgccaa
      901 tactttttct tttttttaat aaatgcaaac agacaccttg aaatccttta gaaaaaaggc
      961 ttctataata ttttgtgttg ttggaaaatc gaaggcgatt ttctactaac actttttctt
     1021 cgtttcaact caaaaagaga ctcacttttg gtaaaattta gtcgaccaaa acatcaccct
     1081 accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta acagataaac
     1141 atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt caagcgcagt
     1201 ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga tcgattcgta
     1261 ttatccgcaa cggttcctat acgacacgta ctcagatgtt gaaagtcaaa gaaaagttga
     1321 ctattttaga attaaaacgt acccgttttc tatgctatga ttgtgggcag accttttctg
     1381 cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag caggcgattc
     1441 tcatagaact gtacgaaaat caatcacgta atctgaaaat cagaagaagt accgtcggct
     1501 caagcgatat tggaaactct tactcaaaga ttccacgaca ttggagccgc tcaaacggca
     1561 ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgtcgatg aaccgttgtg
     1621 cacaatggtg tcctgaacgt acagcgacac cttccatatc cagagcagtc gccacatcac
     1681 ggggatggat gccatccaaa ttaaaagcaa ccacgccagt atgatgttca ggatcttgcg
     1741 gtccgtaaat ggtgataccg ccgatcgctt gtaatttagg taaaacgtag gcaaccagtt
     1801 cttgctcata acgatgaata ttctccatac caatctcatt caagtaatcg attgctcctg
     1861 caatgttcgg agtaccagct tcaaatttcc atggcagctc tttccatgaa ctatcgtaaa
     1921 gattgacaaa atctatcatt tcaccgccga attcgactgg ctccatttgt tctaacaatt
     1981 gacgtttgcc atataataca ccgattcctg tcggcccgca cattttatgc ccgctaaagg
     2041 cgaaaaaatc tgcatccaac gcttgtacat caactgtcat atgcggtgct gcttgggcac
     2101 cgactggact atcctcaact acaacagcct gttcaaccct cagccgtttc aagcacaaca
     2161 aataaccttc tggatctggt ttgccgacct gaagatcatc gatcgtcaca aaggaatcaa
     2221 ttttttcctt aatcatcgga tgtgcttcaa aaaaatagtc tagctcccgc agttcagaat
     2281 tgagactact ccgattttcc agtgctgctg atccgctgct caaaatgttc agcatccact
     2341 ggtctcccaa ccgcttgact tatcccttca taaaaggaca gttctgctgc cgtcccaaca
     2401 ccttgttgat agtctgcaaa aggaatttta tagccgaact gctccagtaa ataggcttca
     2461 gtaaatagtg atgaatactc tcgtaaaaca tccattcact gtcaatcatc gtcccatcaa
     2521 aatcaaaaat aagtgctttg gtcatcttct ttccccctta cccaaaacaa gcaaaagctg
     2581 tgtcctgctc tctctacagt atcagcataa aaactgccgt ctgaaaagtc aaaggtgaaa
     2641 gaaatgtggc gctataattt ctaggactga tagtaaatca ttctatcagt ccttttgagc
     2701 ataaaaaaac aaaacataca gaagctccgt tagaattgaa tcaccacaac acaatcaaag
     2761 gagcgttctg tatgcctgat cttcatctta tcaaactatt actaggaatc aaagatggaa
     2821 acattcaaat cactcatgtc gagaagaaaa aaatacattc aatcaacagt atcgttgttt
     2881 ctgccacgct cattaaaacg attcagccct gtcctcagtg taaacaggtg aatcaacatg
     2941 ggctgatcgt taagaacgga aagaaaaaat cgctggtcct tctcaataag tgtgcgaatc
     3001 aacggactta tcttgctttg gcaaagcaac ggtacctctg tcgtggctgt cacacctatt
     3061 ttacggcgaa tacctacatt gttgaaaaaa actgttttat cgccaaacaa gtacattata
     3121 aaatattgga ggaattgact gaaaaacaag caatgacgac gatttctaaa cattgcggcg
     3181 tttcttggtc aaccgtttcg cgaacactcg cttctctgct ccccatgaca aacgtgaaaa
     3241 taaattggtt gcctcgctgt tcattggtag atgaatttcg ttcattgaaa aatcaagtcg
     3301 gtccctattc tttttcttgt atggatggcg atactgggaa attactggat attcttcctt
     3361 cacgtaagaa gaaagatctc atttcttatt ttatgcaatt tgaacgacgt gcacgtttaa
     3421 acgtaaaaat actcgtaaca gatatgaacg cctcttatgc tttgctgata aaagagtgct
     3481 ttcctcatgc caaactcgtt gtggatcgct ttcacatcgt caagcacctc attcgcagtt
     3541 ttaaggatat ttgactatga gtcatgaaaa gcttcgaccg aaacgatcca atacaagcca
     3601 aacactatcg acaagtgaag gctttatctc gacttctgat caaacgacag gacacgcttg
     3661 tttatgataa atggacaaaa tggcgtaatt ttggttgggc ctatttaacc gaaagtgaag
     3721 tcgtagaacg tctactttct acttctgatg aactacgaat agcttatgct tactatcaag
     3781 aaattcttca agctttttat gacaaagaag cggacatctt ctttcagctt gtaaaaacaa
     3841 tgcccaagtc ggttccaaga gaactgcatc atataaaaaa agcgtttata aactatgaat
     3901 ccggcattcg tttagcctta gagttgcctt actcaaatgg caaaatagaa aatctgcata
     3961 cccacatcaa agcattgaaa cgaatagctt acgggttagg atgagaacca gaattttctt
     4021 aatcaatcat ctgatcactt attaaacata aaaaaacatc taaagcagaa aatcaattct
     4081 accttagatg ttcttatatc actctatcag tcctatttga caaagagcca gaaatgtttg
     4141 ccgactatac actagctttg ttgtgcaaaa tgctgagtat caatttccac agggattgct
     4201 agaatatctt tcagctgaat gtataaggca tcgctaattt tctcttcccg atcgttcaac
     4261 cgatccacta aaatttcttt gccctccagc gtttgaaaaa cataccgttg aatgttgcct
     4321 aaaataaatg attccttcca tagaaccgga atcttaaccc cctgaggaac cggttcaaat
     4381 tgaatcagct ccggtcttaa ataataaatg cagcgattgc ccttagcgga aaactcgtgg
     4441 gtatactgat tcagctcata ccattcgaaa cgattatagt tgccgataaa ttccgcaaca
     4501 aatcggctct gaggattctg atacaattcc ttcgaggtgg aaacctgagc cacccgccca
     4561 gcttccatca caaaaatccg atcactgatt cgcatcgcct cttcttggtc atgagtcaca
     4621 aaaatcatcg tcatccctag cgattgctgg atagcgcgca aatcccgctg cagctgtttt
     4681 ctaattcggg catccaaagc acttaatggc tcatccagca gcaggacttt cggttcagtg
     4741 accaatgagc gagccagtgc cacccgttgt cgttgaccgc cagaaagaaa ctggacattt
     4801 ttatctgcct gctccgtcat atccaccaat gatagcatct caaaaacctt ttcctgaatc
     4861 acctcaggac tcagcttctt taccttcaaa ccaaaggcta cattttcaaa aacagtcata
     4921 gttggaaaca atgcatactg ttgaaaaaca aagccgatat tgcgatcctt cgtaggctta
     4981 tcggcaatat tctcattgtc cagcacaatc tcaccgctct ttggttgttc caatcccgtg
     5041 atacagcgca gcaacgtaga ctttccgcac ccgctgggac ctagtaaagt aaccagctca
     5101 cccttttcaa tctgaatttc atcaatagct aaaatctgtt tttcatcaaa aaacagattg
     5161 agatcatgta tttctaaaaa cattttccgt cacaactcct ttaagggctt ctttttcgac
     5221 tgcttctctt ccctcgtctg cttaagggac agctgttgct tttgtcgata agtcagcata
     5281 aaggtagtta aagcaattcc caccataaac agcaaataca aaaccatcac agcgctagcc
     5341 aaatggccgt tttcgttcat ccgccgcagc atatagatgc gtaccgtctg aaatcgaccg
     5401 ccaatcaaca ggttcgtcag catgtactcc ccaaaaaccg aagaaaaaat catcagagaa
     5461 actagggtca caccgatacg gatgtttgga aacagcacct ggataataat cgtagacatc
     5521 ggggcgccca aggtttctgc tgcatccact aattccttca gattaattaa gcgcatctgg
     5581 ttgttaagac taagatagac aatcggcaac gccgtaataa acaatgcacc taccaacact
     5641 acaaacattg ggataccagt ttcagcatac gttcgaagca aagccgttac caaaatcact
     5701 cctggtaaag catacggcag caggagcagc ttttccagcc aacgattcaa acgagggaaa
     5761 tacaaatgaa tccaaataat ggtgggaatc atcaacaaca gaatcgtcac caagaccaca
     5821 cctgctaata aaagtgagcg acccaccgca gcaataaatt ccctgtccgt cagcagctgt
     5881 tggaaccatt gcaaggtaaa atcactaggt aaaagcgatt ttgaccattt agtggagctg
     5941 gcatacaaaa tcgttccaat aatcggcagc aagtaataca ggacaaagca gaacaaaatc
     6001 ggttttatta actttttcac gtctgcacca ccttgccttt cttttgaata atcaacattg
     6061 ccgttaccag caggacacct aaagcgactg ccaacgcact gcctaaatta ggcttggcga
     6121 acacatctcc cgacactaaa gccgcaattc gaatcgttaa aagatttaca ttactgctgg
     6181 tcagggcata cgctgtttca taggtcccca tgccattagc aaataagatg acaaaggtgc
     6241 tggcaatcga tggtcgcaaa aaaggcaaag ccaccttgaa ccaagcatac gctgatgaag
     6301 cgcccagcaa ttgtgcggac tccagccatt cctttttcac ttccttcatg ctgggataca
     6361 aaaacaagac ccctaacgga atttggaaat aggtatacgt gatggtcaat ccccaccaag
     6421 aatacaaatt gaagtccgct agaaatggca aaatcatttt cagcacacct acattcccca
     6481 acaaaataat aaaagagaat gccagcggaa tcccagcaaa attagctgcc aaattggaaa
     6541 caaaggtgat tttctcctgc aatttagtcg atagattcat taagcataaa cataaaataa
     6601 acgtcccgat taacccaatc acactggagc tgacagcaat cagtacgctg ttaacaaagg
     6661 cctgtgtgta atatttgttg ctgaaaatat cactgtaatt ggccaatgtc cactggttag
     6721 ttaaatcact gtgcaagctg cacagaacca taaaaagcag cggcagcagt aaaaatgcac
     6781 agaagataaa ggaggcagga accagaacca agcgtttttt catctctttc atcccctcat
     6841 caattcttga atgtcctgca ttttctctga cggtgcaatt cctaataacc agcaaaacaa
     6901 tggagccgcc agcagctgtg gtacagtttg ttccttcagc ttttggcgtt tatttgaaat
     6961 caaataaaaa ggaacttcac gatgtgcagc caagctgccg ccatgcagtc cgtgcgcatc
     7021 catcccgtga tccgcagtca ccacgacttg ataacctttt tgcagccatt caggaaggta
     7081 aatagccaat aatctgtccg cctgattaac cgctgctgca tattcatgag aatcggcggt
     7141 aaatttgtga ccgacatcat caatattcat cgaatgtacg accaaaaagt ctggctgata
     7201 ctgattcact aaataatggg catccgcaaa taaatgcgaa tcagggtaag tgtcctcact
     7261 ataaaagatg ccatgctgaa tcagtcgctc ctcctgatgc tggatgcgat caaccaaacg
     7321 attatagggg gcttgattgt acaactcgct gtaccagtgg taagcggcgg ctcctgtttt
     7381 tccgccagct tccttaacta attcaaacag cgaccactct ttggatcgct gagcatggta
     7441 attactatag atgccatttt cataggctgg aacgccggtc atcagtactt cgtataacgg
     7501 acgtgaattg ctgggcagct cactttttac cttcaataag cttgcttgct ggtgctcaac
     7561 taaatgattc atgaacccaa gctgggcgac ggctgtgtcg tagcgacagc cgtctagtat
     7621 aactacaatc agtttgtcaa gtctactttg cataggtcag tacctccgcc tgccaaagct
     7681 ctggaagctc tacagaggtc ttgttccaag cttcattgtc ttcaactggc accgccgctt
     7741 cataagcatc ctccggcagt aatttttcag ctacttcttt aggcagctct accttgcgaa
     7801 taggtcgcgc atagcccttt gccaaattga tttgcccttc atcagataaa atatattccc
     7861 gtgctagctt tgccgcattg ggatgagggg catttttatt gattaaggtt gtatacccgc
     7921 taatgatgga gccgtctgtt ggcacagtta cctcaaaacg ctcggagtca atttggtcac
     7981 gataatttag tccattgaag tcccaaacaa ctgccacatc gatttcacct ttttcaagat
     8041 ttgctacgct ggcatcaacg gtggacaaac ggcctttttc tgccattttt ttgtagtaat
     8101 tcagacctgg ctgaatatcc ttttcgtcac ctccgttggc aatcgccgca gccaatacag
     8161 caaattgtgc ttggttggca atcgtcacat cccccacgct aacttgatag tcaccctctt
     8221 gcagctcctt ccagctagtc ggtgcttgct tcacattctc tttatcggtg ataaaggaaa
     8281 tggttcccgt atagcttaac aaccaatccc catcatcatc ctttgcccaa tccggaattt
     8341 cctcccaata gttggtctta taaggtaagg ttaactcttt gctttcagcc aacggaccga
     8401 agctgatacc gacatcacca atatcggcgg ttccattttt tccttctgat tcaaacttag
     8461 ccaattcttc tgcactggac atatccgtat ccacgtgact taagccgtat tctttttcaa
     8521 ggtcgttcca agtgcctacc caatttgccc aagtatccgg cattcccaag ctggcaatct
     8581 ctccttcttc cttggctttt tctataatcg tatctaaaga gtcgtctgcc gcagccgatt
     8641 gcttcgttgc ttcacctgag ccgcagcctg tcagcaggat acttactgtt gctgcacaaa
     8701 ataaaccgat agtcatgtta cgaatttttt cattcatttt cattcattct tctccccttt
     8761 cgataattcc attctaacaa agctttgtaa tgagagaatc agtttcttga aaaggtttca
     8821 tgaacaatgt aaaggttgta tatggtttgt aaatttcgac gaattcccgt taaccttcta
     8881 aagcttttgc ggtatgatag tagaaaaaag gaggctccct atgcagtatt atgatcagca
     8941 tctccacacg tatttttcct ttgattcaga ggaaaaattt gaaaactatt tagcctatca
     9001 gccggagttt ttcgtttcta cggatcactt tgatttaaac aatccatcta ccggaaaaga
     9061 tgacattccg gattatccag cctatataaa caaactggac tctttaaaag aaacctattc
     9121 caccaaattt ctgcgaggta tcgagattgg cgttgttcca ggacaagaag agcggatcgt
     9181 ggattacctc gctcagcatc cttatgattt aaagctggta agtattcatc aaaatggccg
     9241 ctttgattat atggacgatg tggttttaac caaagatccc attcaaacta ccaaggaata
     9301 ttttgatcag atgttgcagg tcttgacgga ttttccagaa ggagatattt tgactcactt
     9361 tgattatgga ttacgccgct tcaccttcac agcggaagaa ttcgcggagc attttgaggc
     9421 tcagctgcta acgattttca aaaaggttgt tgaacgagaa ttggcgatgg agttaaatgc
     9481 caagagcttc ttgaaatacg gcaacgaaga cctttaccgt tatgccgttc ctttgtatca
     9541 aagtgtcggc ggaaagctct ttaccttagg ctcggatgct catgttgccg aagattatca
     9601 gctgggcttt aaaaaaatga gtcagctgtt ggcagactgt caggtagaaa agttgctagt
     9661 cattcaagga aaagaacgcc gcctgacgcc gctgccgatt ttgtgattcc tatataatag
     9721 aagaaagtgg tgaaaaaaat gagcaacctg tgggactaca atcaagaggc acccatccat
     9781 tatttaattg cgcgacattg ggatgcgttg aaaattgagg cggtttgccg atccttgtta
     9841 gcagccgttc caaaacaaca actggaaaat tttttggtgg ctgacagctt gcagcgggaa
     9901 aaagttcagg cttattttgc agcctttaaa gatcagcctt tggaatattt gcatgcgcag
     9961 tttcatcttt tttatcaagt agccgcccca gatgactaca acgatttacg aggacagctg
    10021 caattgacct ttcaagcgga tgaaaccgcc tataccgttt tattgggaat ggctcgcctt
    10081 ggtgatcagg cgaaggtcga gtggcggatt ttcgatattt gatggcagga tcaaggcgtt
    10141 tcagttgcaa accttcctct ctttcaatta gaaaaaccgc agcaggaaga agtttgtgtg
    10201 ctggtcaccg atgctggtga aattcgtctc cgcttatttc ctgaccaagc tcccctggct
    10261 gtagccaact ggcgctccct agctgagcaa ggcgtttatg acaacacacc tttcggctcg
    10321 tgtcattaag gaatttgtta ttcaaggtgg cgctttggat ggttctggtg atgaagctgt
    10381 ttccagctat gagggctttt tcgctgatga agtgcatcgc ggactttatc attttaatgg
    10441 cgccttggcc ttaggggacc acggccccca caccaacggc aaccagtttt ttattgtgca
    10501 aaacacaaag gcgcaggctg acctgctaat gtaaagcaag catacttgga gcttggagga
    10561 ctaccggagc tggatggtcg ttatactgtt tttggacagg tataccaagg gttagaaatc
    10621 gtagcagcga tcgcagctca gccgacaaac agtgaagatg taccgattga gccagtgttc
    10681 attcgacaaa ttaacttcga aaaaacatcg taaaaagaca ctcactgact tcagcaaaag
    10741 ctctattctt gagctggctg cagctagtga gtgtctttca tttatccata agataaagct
    10801 tggattcaat aaataaataa atgttttggt taaagctgtg accagcaaat gaggaaccgt
    10861 gcgagccagt tgatctgcca atgcaaacat ttctttaggt gaaggcgctg gcgtgtctgc
    10921 caacggatac tccagtccca atgcttggta accgtgcata ccatagcagt ggtacggtag
    10981 taattctaac ggtacgaacc gatcaagatc tgccacaaaa caagcaatcg tcagcagctc
    11041 ggtcaggttc atattgatac ctggaaccac aggaactcga attcgcactt cgcagttagg
    11101 atactcaatt aaaaagcgac gaatattctt caaaatttgt tggttatcta agccggtcca
    11161 ttgtttgtgg agcttgctgg taaacatttt tacatccaca aacaatgtat ccacataggg
    11221 agctaccttt tggatttcat tataagcacc acaaaagctg gtttccagta cggtattgat
    11281 tccaatgtat ttcgattttt gcaaaatttc tttagcaaaa tccgcctgaa ccagtgcctc
    11341 gcctccgcta atggtcactc cgccatccga gtgaaagtaa aaaacagcat ccttctcaat
    11401 ctcatccatt acctcttcag cggtcatggt ctggccaaat cctttttcaa tctgcttgct
    11461 ttgtgattcg ggaatcgaac accaggcaca gcgtaatggg cagcccttta aataaacaac
    11521 ggtccgtaaa ccagcaccat catgtagaga accctgttct attcttaata ctgttccttc
    11581 catatagatt tttgaattac acactagttt aaactaaaac acacaaggaa aaacttacca
    11641 tttaatcata aaattagctc atataaatat actaggatgt aaaatagact ggtcaaaatc
    11701 tggtcaaaaa agaggactaa tacaggtagt cctctttttt gttattttaa tattctaaat
    11761 cattttttat tggtttattc ccttattcga tgtagactca aatcactata tcactagata
    11821 tcaaacggcg tcatacggaa tcaaaaacat caaatttgac ctgttaaatg gtcattgaat
    11881 aacattcaaa atcaataaaa cggagtcaga cacggagtca aaaacgctga gagtaaaaaa
    11941 tatatacagc cttgtaggta aagtaccaca gggctgtata ttttttattt cagattacct
    12001 taaatgattc catttacttt ttatctcatt ttttcctact agttagtatc aaatactctc
    12061 ctattttctc aataaactat ttttggatac atggtatctg aaacagctaa aaccttgtca
    12121 ttacagcaaa aaatcacgtt atccagactg tagacaaagt ccattttatg gatgatgtct
    12181 acagtctttt cttttataat agatttatga aaacgaatgc aaggagtgat agctatgatg
    12241 tctaaaaaca atacgaatgg tagaaatcaa tttgcgatgc ttacgattga tgatctagtt
    12301 cctcaagatc atctcgttcg aaagattgat gccgcgcttg attttgaatt tatttatcca
    12361 atcgtcgaag ccacttactc cgatttaggt cgcccaagta tcgatccagt gattctgatc
    12421 aaattggtgt ttattcagta tttattcggt atccgttcca tgcgacaaac cattaaggaa
    12481 gtcgatacca atgtcgctta ccgctggttt ttaggctact
//
LOCUS       DAZKMZ010000073        12491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000073 DAZKMZ010000000
VERSION     DAZKMZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.073"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8M5_002131"
     CDS             <1..375
                     /locus_tag="ACG8M5_002131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359369.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8M5_002132"
     CDS             complement(324..893)
                     /locus_tag="ACG8M5_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0359370.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8M5_002133"
     CDS             complement(967..2256)
                     /locus_tag="ACG8M5_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359371.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8M5_002134"
     CDS             2514..3458
                     /locus_tag="ACG8M5_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0359372.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8M5_002135"
     CDS             3472..4545
                     /locus_tag="ACG8M5_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0359373.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8M5_002136"
     CDS             4535..6706
                     /locus_tag="ACG8M5_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0359374.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8M5_002137"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8M5_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0359375.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8M5_002138"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8M5_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0359376.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8M5_002139"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8M5_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0359377.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8M5_002140"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8M5_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0359378.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8M5_002141"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8M5_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3519 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa a
//
LOCUS       DAZKMZ010000074        12337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000074 DAZKMZ010000000
VERSION     DAZKMZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.074"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8M5_002142"
     CDS             <1..154
                     /locus_tag="ACG8M5_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0359379.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8M5_002143"
     CDS             complement(208..486)
                     /locus_tag="ACG8M5_002143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359380.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8M5_002144"
     CDS             complement(601..1218)
                     /locus_tag="ACG8M5_002144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359381.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8M5_002145"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8M5_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0359382.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8M5_002146"
     CDS             2780..3196
                     /locus_tag="ACG8M5_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359383.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8M5_002147"
     CDS             3193..3663
                     /locus_tag="ACG8M5_002147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0359384.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8M5_002148"
     CDS             3677..4450
                     /locus_tag="ACG8M5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0359385.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8M5_002149"
     CDS             4464..5276
                     /locus_tag="ACG8M5_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0359386.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8M5_002150"
     CDS             5330..6295
                     /locus_tag="ACG8M5_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0359387.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8M5_002151"
     CDS             6431..9154
                     /locus_tag="ACG8M5_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0359388.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8M5_002152"
     CDS             9167..9799
                     /locus_tag="ACG8M5_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0359389.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8M5_002153"
     CDS             10007..10231
                     /locus_tag="ACG8M5_002153"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0359390.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8M5_002154"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8M5_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0359391.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8M5_002155"
     CDS             10556..10825
                     /locus_tag="ACG8M5_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0359392.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8M5_002156"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8M5_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0359393.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4030 a         1998 c         2422 g         3887 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgat
//
LOCUS       DAZKMZ010000075        12209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000075 DAZKMZ010000000
VERSION     DAZKMZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.075"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            434..664
                     /locus_tag="ACG8M5_002157"
     CDS             434..664
                     /locus_tag="ACG8M5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359394.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            823..1656
                     /gene="terS"
                     /locus_tag="ACG8M5_002158"
     CDS             823..1656
                     /gene="terS"
                     /locus_tag="ACG8M5_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0359395.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            1649..3064
                     /locus_tag="ACG8M5_002159"
     CDS             1649..3064
                     /locus_tag="ACG8M5_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0359396.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            3076..4605
                     /locus_tag="ACG8M5_002160"
     CDS             3076..4605
                     /locus_tag="ACG8M5_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0359397.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4523..5575
                     /locus_tag="ACG8M5_002161"
     CDS             4523..5575
                     /locus_tag="ACG8M5_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0359398.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5577..5894
                     /locus_tag="ACG8M5_002162"
     CDS             5577..5894
                     /locus_tag="ACG8M5_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359399.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            6021..6644
                     /locus_tag="ACG8M5_002163"
     CDS             6021..6644
                     /locus_tag="ACG8M5_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0359400.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            6659..7549
                     /locus_tag="ACG8M5_002164"
     CDS             6659..7549
                     /locus_tag="ACG8M5_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0359401.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7572..7787
                     /locus_tag="ACG8M5_002165"
     CDS             7572..7787
                     /locus_tag="ACG8M5_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359402.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7799..8140
                     /locus_tag="ACG8M5_002166"
     CDS             7799..8140
                     /locus_tag="ACG8M5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359403.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8140..8511
                     /locus_tag="ACG8M5_002167"
     CDS             8140..8511
                     /locus_tag="ACG8M5_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359404.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8504..8899
                     /locus_tag="ACG8M5_002168"
     CDS             8504..8899
                     /locus_tag="ACG8M5_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0359405.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8901..9278
                     /locus_tag="ACG8M5_002169"
     CDS             8901..9278
                     /locus_tag="ACG8M5_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0359406.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9279..9887
                     /locus_tag="ACG8M5_002170"
     CDS             9279..9887
                     /locus_tag="ACG8M5_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0359407.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9887..10228
                     /locus_tag="ACG8M5_002171"
     CDS             9887..10228
                     /locus_tag="ACG8M5_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359408.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10470..>12209
                     /locus_tag="ACG8M5_002172"
     CDS             10470..>12209
                     /locus_tag="ACG8M5_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0359409.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGG"
BASE COUNT         4422 a         1696 c         2512 g         3579 t
ORIGIN      
        1 aatgattcca ttaataattt caatttttgc gctttgtctt aatgtatata tgattggatt
       61 taaaaatggg caaaataaaa aatagtagca gccaagaata attttatagt gtcactgtgg
      121 cggaaagggt agacgctaag catgtgtgct aggtcaatgc ttcggcaacc atgcaaggtt
      181 cgattccttg ccagtgactt tggcaaccga ggcatcggcg gtttaaaaat ataggggtgc
      241 gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat tacataactg gttaggttag
      301 attgagattt gggattcggt acaaatgaat cgtcaaatgt ctcaagcaca ggatcggaaa
      361 cgtccctgcc tgtgcattac atattagatc actctttgag tggtcttttt ctttacataa
      421 aggaggctac ataatgagaa actactggta tgtatcgcta actaatgaat atcctcgaac
      481 cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa atcaaaggga agtactctat
      541 tgtcgaaatg gggagagaag cagaaccatg tgagattgat gcgtgcaagc tcgtttattg
      601 cggtcatggt ttctatgatg aaccaaacat tcaaaataat attaacaaga atttgaggga
      661 ttagaatgca agaaatggct taaaaatctc caatcaggac aaatcaagtt agacaaagtt
      721 tccgatttaa agatattgat tgaagctgat ttaatgctaa aagatattga aaattagaaa
      781 acaaaactca acctaaacag aatgtgaggt ggtgtgtatt gaatggcaag acaacgtgat
      841 ccaagacgtg atgaagccaa aagaatttgg ttagaatcca acggagaaaa gcagttaaag
      901 gaaattgcat ctgaattaaa tgtttcagat tctcaggtta gaaaatggaa atcgcaagac
      961 aaatggagcg ctgaattgat tagtaacgtt accaatggca aaagtaacgt tactaatcaa
     1021 ggtggcgctc ctattggtaa tcaaaatgct aaaggtaaca aaggaaatag ccgagcttct
     1081 ccgccagtgg gtaataaaaa cgctttgaaa acaggtgagt atgaaacaat attttttgac
     1141 acgttaaccg ataatgagaa ggacatctat tctagtttga atgatgatcc ttcttttgct
     1201 ttgtccgaag aaatacgact tcttaaaata agacaacttc gaatgatgaa aagaatccaa
     1261 caagctgaag ctggactaaa tgatgaagaa gtcgaacgat tgcagcagct aagaaagatt
     1321 aaaactccga ttgaaaaaaa tggtaaaaag ctagaaatca agcgtgaggt tatgcaagat
     1381 gtgcagatta gcagaaaaaa acatcgcaaa attgatgata ttctttcaat tgaagattca
     1441 ttgactcgga ttagcaacca gttagctaaa gccatcaagc aaatgaatga actttatatg
     1501 aatgaataca gaactgattt aattaaagct cagactgata agatccaagc tgagacaaat
     1561 gaaattggcg gaaataattc aggtgaagaa atagaagaat ggaaacaggc agttttaaat
     1621 gccgcaaaca aacgggcggt gaaagaaaat gaataatgaa tttattcctt ttgctgatat
     1681 tggttctgcc attgattatt actatgataa accagtagct ttttgccaag atattttgca
     1741 tttgaatcct gatgaatggc aagaaaatgt tttaaatgat ttagctgaat tttcaaaggt
     1801 ttctgttcgt tctggtcaag gagttggaaa aacagcatta gaagcaggag caatactttg
     1861 gttcttaacg tgtcgaccct acgctaaagt aatagcaaca gctccgacaa tgaagcaact
     1921 ttacgatgta ctttgggcag aggtagctaa atggttaaat gatagcttga tcaaaaactt
     1981 actgaagtgg acaaagacca aaatttatat ggttggtgat tcagagcgtt ggtttgctac
     2041 ggctagaaca gcgactaaac cagaaagtat gcaaggtttt cacgaggacc atatgttgat
     2101 tgtggtagat gaagcttctg gtgtgtctga tccaattatg gaagctattc ttggtacgct
     2161 atcaggtttt gataataagc tgttgatgtg tggaaacccc aataatattg aaggtgtctt
     2221 ttatgattcc cataattcag accgtgataa atacagagtt cataaagtat caagctatga
     2281 tagtaaacgt acaaacaaag acaatataga aatgattctt aaaaaatatg gaaaagaaag
     2341 tgatgttgct cgtgtccgta tttttggaga atttccgaaa ggtgcgttgg attcatttat
     2401 cagtcttgaa acagttgaat tggctacaga aaaacaaatt agtgattctt tagtcaataa
     2461 aacaaaggtt gctcatattg gtgtcgacgt agctcgatat ggtgatgatt ctacgattct
     2521 ctttcctaga attgctacca gggcattgga gtatgagaag tattcaaaac gtagcaccat
     2581 ggaaacaaca ggatatgtca tcaacatggc caagaatcta atgagtcaat atccgagtat
     2641 tgataaagtg atgattaaag tcgatgacac tggtgtcgga ggtggtgtaa ccgaccgcct
     2701 agaagaactt atagaagaca aacattatcc ttttgaggtg tttggagtga ataacggttc
     2761 aacatcagaa gacgattttt acgataattt aggtactcaa ctatggggaa acatcaagga
     2821 aatgttagaa gaaaatatga cagcaaatct taacggagaa cagcctgtta ttgaattgcc
     2881 ttctgatagt tcgttaatca aagaattaag tactcgcaaa ttcaaaatga caagtagaag
     2941 tcgtatacgt ttagaaagta aagatgatat gaaaaagcga aatattggta gtcccgatat
     3001 tgctgacgca ctggctttag cgttttatga gccaccaagt cactatcaat ttattcaatt
     3061 ttaggaggtg agcttttgat tagtacagta ttatctttag agagatataa gaagctacgt
     3121 gttaaatatg caacacaaat tgaagatgga atgtttgatc cgaatggttt tatagaagat
     3181 atgaaaccat tttttgctga tcgcgaaaga aaatatctag cttatactag cgaaaagaat
     3241 gaaatagata atagaccaaa gcctaacact gatattgtaa aagttaataa taaacttcat
     3301 gctggtatgt attcaattgt cgtagaccaa gcagtcaatc attttactgg tatacctatc
     3361 aaatgggatt acgatgtatc ggaacaaaaa agaacactca ttcaaagatt aaaagataaa
     3421 ttcttaaaaa atgatataga acttccgaca gttccagaag cttttaacaa attaacaagc
     3481 aatcttgatt ccatgagatt cgcaatgctt gattctgaaa ctgcaacttt tcaaggagct
     3541 tgtggagtag ctttccgatt gctagagcct gtggaagaag atgatggttg gaagttaaga
     3601 gcaagtaata ttgaaccttg gagagcagaa agatatggaa atgccggaat ctatatcaaa
     3661 gaaaagtatg actcttacca gaaaaaattt tttcaagaaa tgaaagttat gacaagaaat
     3721 aaaattctga catatgcttg ctatggtgat ttgaattttg ttacaagcgg aacgttcaaa
     3781 aaaattgatg agaccgataa ccctttgggg acgattacct tggcagaatt taaaaataat
     3841 acgaatcgtt attgtgattt tgaagtagct gaggaaattg gagatgcaat tgatcgtgct
     3901 ttgtctgatc aacaaaacga aatcgaacaa tttaagcttg cgtacatgct tgttactggc
     3961 acaacaatga gcaaaggcac tgctaaagaa atgatgaatc agctagggat tatcaactta
     4021 aaagccccta ctgcaaaagc tgaatatgtc acaaaaaatt tagcaaaaga tttcaatgaa
     4081 tatcatatgg atctattgaa aaaacagttt tacaccattt gtaaagcaat cgattttaac
     4141 gatgaggtgt ttaaatcaaa cagttctgga gaagctcgca agtggcaaat cattagccta
     4201 gaagcgaaaa caaatactaa agagcaatat tttagagaag gattgaaaga atgcgcagaa
     4261 acaattgctg cttttcttaa gtttcatgac aaagtagaaa ttgagcctga aaaaattatt
     4321 tttactttct ctagatcttt gccaactgat ttaagttatt tagctgaagc tttacctaaa
     4381 cttgcaccat acgtatccaa acgaactatt cagagccaaa ttccatttgt aacagatgtt
     4441 gattatgaaa atgaaatgat ggaattggaa agtggaagtg cttatccaga tagtgaatac
     4501 aattttggcg gaggtggcaa tagtgacgat agaaacgaag tattggacca aacgtcgaga
     4561 actggaggat caagcaaggc tcaaacaaga aaatcagaca cttaaaaaat taactagtgt
     4621 atttcctgaa gcactgaaag agatacaagc aaaactatta tcacaagctg acttacacaa
     4681 tatcacttat ccagaaatga tggagtttta cagtacaagt aatcagaaaa aatatcgtga
     4741 atatgtggaa aaaaattata agtcattaaa aatgtatgat gcaaaataca aagagtttat
     4801 cgatgaattt tttccaccat ttgactatgc aaaagtcaat cgcttattac aaatacgatc
     4861 agatgtattt aaaattcttg cagaatatgc gatggatgca gatgtgaatc aatatttttc
     4921 tgatcgctta gaggaaattc ttcaaagaac atattcttct aatgctaatg tttttgttca
     4981 acttttaaac gttgatatac caaattattt accagaaaat gaaattaaac attatttgaa
     5041 ctatccatgg tcaggaaaga cattttcaag aagactttgg ggaaatattt catcgcttga
     5101 acagaaactc tctaatgcta ttgtaaaaag tgttgctagt ggagaaggcg ttatacacgc
     5161 attaaacacc atgagattag actcagaaat ttgtgacatg tttaagttag aagaatcaaa
     5221 gtataacaaa gcgatagaaa atctcgttcg aacggagtat gcaaaatttg cacaagatgg
     5281 tattgaaaaa tcatatttag aaacaggtat tgaggaatac aacgtattga ctgcaaaaga
     5341 tgagagagtt tgccggattt gtggaggaaa ggcaagtaag aatccctata aactgaaaga
     5401 tgctgtcata ggtgaaaatc gagcaccttt ccatagtcgt tgtagatgta cggatgttcc
     5461 taatttacca aaattaggaa aggatattga tgaagaatat gaccgtttat ttggcgattt
     5521 attagatgag tttgcacatg attcttttgg aattaatttg aaacggagga agtagaatga
     5581 aagatttttt tgaagcagta ctaacaatta atgtaaatgc tgatattgca gaagcctaca
     5641 aaacagctat tgagtctgag aaccatccta atggcttgag agaccattgg aatggcaatt
     5701 atgcctacgt ggttattggc gatcaaactg ttaattatca agataatact ccagttgata
     5761 agaataccgt taatttaacg attcaattat tatctcattc attaccaaat ttaaaagaaa
     5821 cagttgattg gtatgaaaag atgggatgta ttgttgttag aactgactac aaagaaggaa
     5881 agtctagtaa ttaggctttt ttattttgtc cgaaatgacg ttaaactagc gcaatactgg
     5941 gcttggttga atggtggggc gcaactatta aaactcaaag caatgcgggg cgtgaaaacg
     6001 aatcgtgggg cgaaaggaga atgagcatga aacacaaatc attaatgcca atgaatttgc
     6061 aatactttgc cgaaggtgac gatcaaaagt tttcttttga tgacttcaaa tcttttgtgg
     6121 aatccaatga agaagctcaa aaatttattc agtcacaatc tcaaagtgtt gccgacaaac
     6181 aattggaagc ttggaaacaa aataacttag ataaaattaa acaggatacc atcaaggaat
     6241 atgaggaatc taagaaaaac aagtcacctg agcaaattca actggaaaaa ctacaagctg
     6301 aatttgaagc agaaaaagcg ttgcgtgtga caagtgataa taaagcattt gttgcagaac
     6361 aaattgctgg attagaacta gatggagagt taaaagagtc tatttctcaa tttatgctaa
     6421 ataatcttgt tagttcggat acagatttca ctaagaatgc tgttgaaggt tttacaagtg
     6481 ttttgaatgc aattaatgag aaacatgcag atgcattaaa agaactacaa atgaagtctg
     6541 catttggtgg aactcaacaa tcgaataacc aggttcagca gaacaatgaa acattaacaa
     6601 atccagaaga acaattagga caaattcttc aacaatttaa ctaggagagt gaaaaattat
     6661 gaaaaaaaca tctttaaata atttagagta tttggatatt tcaccagcga ttaatgctat
     6721 gcaagtacca aatacacctt ttttaagcta tttacttggt gctggaaaaa cagagccagc
     6781 aaactcgaca gaaattaaat ggcgtgaata tgatatcaac aacgatgatt cttctgaaaa
     6841 acttgagggc ggagaatatc cagatgctga atcaggtcga acttggttta acaactatac
     6901 tgaaattttt agaaaatcaa cctctgtatc tggtacatta gatgctatta atgtgaatgg
     6961 agtcggaaat gaattaacta atcaagtagc actacgtggt atggaaatga aaattgattt
     7021 gaaccgaaaa ttgattactg gtgtaaaagc tgatgaaaat ggttctaaag gtcgtcgaat
     7081 gaatgggatt ttgaacttga tcaattcagc aaataaggca gaaacagcca ctgcgggtgc
     7141 agtaacaaga aaagatatcg atactttatt taaattgatg tatgaaaaag gttatatggg
     7201 agaaaaacta tgcttgattt ctccagatat gcaggagtta atgactgatg agttagatgg
     7261 aaaatcaaca aaaattgttc agttcgggga aagagtaact tttggattgc aaattggaaa
     7321 tatcgtgtct aattacggta caggtattgc tctactagaa ccatcattgc caaaaggaac
     7381 aattgccgca atcgatacta attatgtgaa actacgtcca ttacgtgaat ggagagcaga
     7441 agaacttgca aaaacaacgg attctagacg tatcggtctt gtaggtgaat actctcttga
     7501 atacaatgct tcaaactctg gggcaatttt aaatttaaag tctgaataaa agggagttag
     7561 tactcccttt tttgatagga ggaattacag tggtaaaaaa agatgaaact aaaaaagatg
     7621 aagtcgtgaa atatagagta ggtaaaacta aaaattttgt tgggtttgtt catcctaaaa
     7681 ctcgtagatt tatcacagca gattcaaata acgaatttat catttctata gatgataaag
     7741 aagcaattgc aattttggaa gatgcaattg atgttaatga aatttaggaa gtgatctgat
     7801 ggatgaatcg ctaaaaacgg aaatcattga gtctacaaaa gaagattttc cagatttgag
     7861 tgaagaacgc ataactaatt tattagaaat aattttgcta gaaattgaat catacaacac
     7921 ttgtaaaaat gatatttcat gggaaaagtt aaaaagcgtg attagtgaag tgttgtataa
     7981 aataataaaa aatgaatcag aaaaaacagt atcttcagtt agacgtggcg atacaacgat
     8041 tagttatgct tcaacaacaa atgatgttag tgagttactt ctaggctatg gtgatttgat
     8101 acgaagagtt attggttgtg gaggattgga gtttttttaa tgaatgaagc agatatttta
     8161 gaaactactt atgaagatag ttgtattatt gaaagactga cggacattga agattctaat
     8221 acaaatatta ctattcaaga ttacaaaaaa gtatatgata atcctatttc ttgcgctctt
     8281 tcacaaggtc aaatcgatgg actagcagtc atagaagatg gagagatggt aaatgtttca
     8341 actgacacat ataaattatt tgttcatcct aagattaaac taaaaaaagg agatcgaata
     8401 acaataactc aaaaggccag tggcttaatt ttttctctat ttgctactaa gcctttttac
     8461 tatcctagtc attgtgaagt gaatttgata ggaagtgaga aaaatgggta atctcaaata
     8521 tgaatcaaat gcagaaaaga tcattgagaa ttttaaaaat atgactgtaa ttgctcaaaa
     8581 agaaggaata tccttcgtta acgattcaat gaataaagtc gttagtttag ctaaaccatt
     8641 aactcctgta aaatcaggta atttaaggcg aggatatcgt gtagtaaaag ctagaaaact
     8701 atcaagcggt cgaattgttg gagaagttat aaacaatgaa cattatttta aatatgtaga
     8761 agaaggtcat aggactaaaa atggtggctt tgtcaaaggg aaattcatgt taactcgtgc
     8821 aacgaatctt gcaaatatgt cttatattcc tcgaagattt aaacaaatgg caataaaaat
     8881 cgttaagaaa ggaaagtaac atgaaagatg aaatcattgc tgcaatcagt agcaaattaa
     8941 aagaaatcta tcctgatggg acaatatatc ttgattcagt tatgcagtca actaaagatt
     9001 tttatttcgt tttatcagta atggaatctg gaactgaaaa tgtaggaatt gatattcaaa
     9061 atgtttcttt cttaattgat attgcattga ttgataataa ccctaataga aatttaatta
     9121 atgaattagt ctcacgttgt gggacttttt ttaatacgat tacaatcgat gagcaaatat
     9181 tatttccaaa agcctattta cctgatgaag cagatggtgt tcaacatatt cgttttacat
     9241 tagaatttcc acaatatatt gaatggagtg aaagataaat gggagaaaaa agaagtaaaa
     9301 ccggaattat ttctgtcgaa aaacctactt ggtttccttt ggaagatgag acagggaatt
     9361 ttccggtata tggtactgcg ttgccaatgg gaacagcagt aagtatcaaa ccaacagcta
     9421 attatgaaac aacacaagat tatggggatt cagtggttca agatcaattt acagcttttg
     9481 gtggtgcaga agttgaactg gaagcaaatg gatatactca taaagtttta tctacaatta
     9541 ctggagggaa aattgttaaa ggtggtgcct tgcgttctgg agaagatatt gcgcaagatg
     9601 gtgcgtttgc atatagacgt aaaaaatcga atgggaaata ccgttatacg gtcttttata
     9661 aaggacagtt tgctctagat tcagatgaaa catcaacgat tgaaggaagt aaagtaagtt
     9721 ttactcatcc aacttggaag ggttcatttg tagatgtacc aggacttgga tatatgtatt
     9781 cagttgatga agatgatgaa ggcgtggata aagcaatgat cggaaattgg tttacaaaag
     9841 tagcaattcc aattgaagaa gcagaacttt caggaggtac agaataatgt caaaatatca
     9901 aacaacgatt aaattaacga aaaaagacga agaaggtaaa tatgagcaag tacaatttaa
     9961 atctgctgaa tttttaccag gaacagttgt agaagatgct gcaggagtta tggaagaaat
    10021 gcaaacagcg actgataagc aatcagttaa aaaagcttta agtcgtgctt attcatttat
    10081 tgcagatact ttatttgagg gacaatttac tggtgaagat tattgcaaag gaattgatgc
    10141 tcgtgagatt gcctcattga caggaaaatt attgaagtct gttactgcag gttttgatga
    10201 aacttataca gagacgaaaa aaaagtaagt gaggcgctca agtcaccttc atttaagtat
    10261 tcgattactt accgagaatt agatataaag acgcaattac tcgaagcagg ttggacgtta
    10321 ccagaaattg aacgtactga cttggatgag ttaatgcgtc tttttgcttt tagagatgcg
    10381 gtaaaagagc atgaagatgt tgagtactat gataatttca ctcaatttta ggaggtgata
    10441 ctttgaataa cgatgaccta attctgaaga tgatactaga tgaatctggt tttactgccg
    10501 gtatgaataa tgctgtcaaa aagttgaact cttttgatga aacaatcgaa agaaaaagta
    10561 gaaatagtgg cagttcatta ggtagtattt ggaaaatatt tgctggaagt tttctagcta
    10621 gcggagtaac tagaatcgta ggagctggct ttgatctaat caagggttcc ataagtggag
    10681 cggttgatcg agtagatacg atgaataacg ctctacgaaa tttccaaaat atgggattta
    10741 gtaattcaga aattatgaag aatatcggaa agaatgggct tttatctcaa ggtattcaag
    10801 gacttcctac tgctttgaat gatgcgataa gtcatgttca acttcttgct tcttctacag
    10861 gtgatttaac tcgttcaact caaatattta aagctttaaa tgatggaatt cttggtttcg
    10921 gtggttcaac tgatcaagtt aatgaagctg ttattcaatt atctcaaagt ttctcaaacg
    10981 gaaaagtaga cgcacaaact tggaactcaa tgatcaacgc tcaacttgga cctactcttt
    11041 ctgctattgc taaaaagatg ggaattacaa tgggagatct gaaagaaggt ttatctcaag
    11101 gtaagatttc tgttgaagaa ttccaaaatc aattaataga aatggatacc aaaggtggcg
    11161 gaggacttaa atcattaagt caaatcgcta aagattctac taagggaatt aaaacctcta
    11221 tacaaaatgc aaaaacagct gttacacgtg gtgttggtga ggtaatagaa ggattaaata
    11281 aagcattagt agattcagat ttaggtggat ttaagggaat tattgataaa gtgggcagtt
    11341 caatggaatc gttcttaaaa gtaattgctg caaatattcc tacagcagta tcatttttaa
    11401 gtaatttatt taacgaaatt caaaaatttg gttctgcttt gaaattcatg atgccctttc
    11461 ttgttcctgc agctaccgct tttggagcgt tcatgtttca acttaaaggt ataccagcaa
    11521 taataaaaag cttcaataat tttaagaatg ccataattgg cgttggaaat tcattaaaga
    11581 ttatgggggc aatagctgct gctaatccat ttgttttaat tattggtgca gtcgttggtg
    11641 caatagctgt tttcggttac tttatggcaa ccaatgaaga gcttagaaac aaggttatat
    11701 ctatttggaa tgatgtgaaa gattccgtaa ttggtgtatt aaaaaatata aaggactggg
    11761 gaattgatac ttggaattct gctaaagaaa tggcatcaaa tgcagttgag ggtgtcaaag
    11821 atgcttggtc aggaacaaag gaatggtttt ctaacacctg gaaaggtatt aaggatgggg
    11881 ccacaggttt atttgataaa acagtagaga cttcaagaaa tgcagtcgat agtgtaaaaa
    11941 atgcatggtc taatatgaag aaatggtttt ctgatacttg gcaaagtata aaagattcag
    12001 ccagagaaaa atggaatgga atcaaaggtt ctattatgtc agttgctggt ccattgatta
    12061 caggtataaa gaatgcattt ttacatgtta ctttttatct agagacctta tggaataatt
    12121 tagttgagat tggtaaaaac gtctttgaga ttttaaaaaa tgttatactt gcgccggttt
    12181 tgtttattac ctccatgatt tctggtgga
//
LOCUS       DAZKMZ010000076        12203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000076 DAZKMZ010000000
VERSION     DAZKMZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.076"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG8M5_002173"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="ACG8M5_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0359410.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG8M5_002174"
     CDS             complement(642..1364)
                     /gene="pyrH"
                     /locus_tag="ACG8M5_002174"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0359411.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG8M5_002175"
     CDS             complement(1500..2381)
                     /gene="tsf"
                     /locus_tag="ACG8M5_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0359412.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG8M5_002176"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="ACG8M5_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0359413.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="ACG8M5_002177"
     CDS             3621..4100
                     /locus_tag="ACG8M5_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0359414.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="ACG8M5_002178"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="ACG8M5_002178"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0359415.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="ACG8M5_002179"
     CDS             5401..5529
                     /locus_tag="ACG8M5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0359416.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5533..6078
                     /locus_tag="ACG8M5_002180"
     CDS             5533..6078
                     /locus_tag="ACG8M5_002180"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0359417.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG8M5_002181"
     CDS             complement(6532..8223)
                     /gene="argS"
                     /locus_tag="ACG8M5_002181"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0359418.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG8M5_002182"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="ACG8M5_002182"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0359419.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG8M5_002183"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="ACG8M5_002183"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0359420.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG8M5_002184"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="ACG8M5_002184"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0359421.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3343 a         2529 c         2203 g         4128 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa cccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaaaaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc att
//
LOCUS       DAZKMZ010000077        12110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000077 DAZKMZ010000000
VERSION     DAZKMZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.077"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8M5_002185"
     CDS             140..460
                     /locus_tag="ACG8M5_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359422.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8M5_002186"
     CDS             912..1112
                     /locus_tag="ACG8M5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0359423.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8M5_002187"
     CDS             1259..4048
                     /locus_tag="ACG8M5_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0359424.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8M5_002188"
     CDS             4095..4622
                     /locus_tag="ACG8M5_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0359425.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8M5_002189"
     CDS             4643..5833
                     /locus_tag="ACG8M5_002189"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0359426.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M5_002190"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M5_002190"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0359427.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8M5_002191"
     CDS             7966..8640
                     /locus_tag="ACG8M5_002191"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0359428.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8M5_002192"
     CDS             8637..8978
                     /locus_tag="ACG8M5_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359429.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8M5_002193"
     CDS             9008..9367
                     /locus_tag="ACG8M5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0359430.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8M5_002194"
     CDS             9627..9749
                     /locus_tag="ACG8M5_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359431.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8M5_002195"
     CDS             9891..11975
                     /locus_tag="ACG8M5_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0359432.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4044 a         2113 c         2603 g         3350 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca
//
LOCUS       DAZKMZ010000078        12080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000078 DAZKMZ010000000
VERSION     DAZKMZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.078"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..963)
                     /locus_tag="ACG8M5_002196"
     CDS             complement(181..963)
                     /locus_tag="ACG8M5_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359433.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1028..1864)
                     /locus_tag="ACG8M5_002197"
     CDS             complement(1028..1864)
                     /locus_tag="ACG8M5_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0359434.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1882..3291)
                     /locus_tag="ACG8M5_002198"
     CDS             complement(1882..3291)
                     /locus_tag="ACG8M5_002198"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0359435.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3305..5128)
                     /locus_tag="ACG8M5_002199"
     CDS             complement(3305..5128)
                     /locus_tag="ACG8M5_002199"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0359436.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5971..6297)
                     /locus_tag="ACG8M5_002200"
     CDS             complement(5971..6297)
                     /locus_tag="ACG8M5_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359437.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6343..6705)
                     /locus_tag="ACG8M5_002201"
     CDS             complement(6343..6705)
                     /locus_tag="ACG8M5_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0359438.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6742..6956)
                     /locus_tag="ACG8M5_002202"
                     /pseudo
     CDS             complement(6742..6956)
                     /locus_tag="ACG8M5_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7084..7419
                     /locus_tag="ACG8M5_002203"
     CDS             7084..7419
                     /locus_tag="ACG8M5_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0359439.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7495..9492)
                     /locus_tag="ACG8M5_002204"
     CDS             complement(7495..9492)
                     /locus_tag="ACG8M5_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359440.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9590..9865)
                     /locus_tag="ACG8M5_002205"
     CDS             complement(9590..9865)
                     /locus_tag="ACG8M5_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359441.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10097..10831
                     /locus_tag="ACG8M5_002206"
     CDS             10097..10831
                     /locus_tag="ACG8M5_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0359442.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10870..12012
                     /locus_tag="ACG8M5_002207"
     CDS             10870..12012
                     /locus_tag="ACG8M5_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359443.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3913 a         2408 c         1846 g         3913 t
ORIGIN      
        1 acttatatat caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata
       61 gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg
      121 tataagtacg gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca
      181 ttattcaata acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac
      241 cacagaataa tcttttttcc cattttcaag taaccgaagt ggaatgtctt taccaacttg
      301 ctctgtcact gcattactga tacctgttag attagctaat aaatctgata aagattgagc
      361 atctgtagaa atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt
      421 ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt
      481 cttcccagta tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt
      541 ctgctccgta actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact
      601 tatatattgt ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc
      661 actataacca gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga
      721 aaaatagaca tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac
      781 ttgagacctt cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt
      841 attattaatc tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca
      901 ctttaccact tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc
      961 caaggaatca ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt
     1021 tcttcattca gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg
     1081 ctgaggcacc catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt
     1141 tcggaaaacg ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct
     1201 tcttaatcat tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg
     1261 aatctttatc aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta
     1321 gtgctcttac cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa
     1381 tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga
     1441 tcaattggat agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa
     1501 tcagcaggcg attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt
     1561 gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat
     1621 aatcgataat tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa
     1681 tgtaatcctt ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt
     1741 cagccggaac caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta
     1801 cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc
     1861 tcattagatt caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca
     1921 aaagaagctt tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac
     1981 atatataaaa ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga
     2041 ccaaggtaaa taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat
     2101 atgtttggct agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat
     2161 gtcgggtgcc ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa
     2221 tttcctcaat gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc
     2281 tacataagga ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc
     2341 ccccgctcta gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga
     2401 ataataatta atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc
     2461 ctcaatttta ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt
     2521 tccccgaact gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc
     2581 aattgcatct tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc
     2641 gatttttcct ttaagattca actgatgata caagtgaatt actctagctt gagctaataa
     2701 aacatggtga ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa
     2761 accaatcgct ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt
     2821 caccttgtca ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt
     2881 tgctcgattt aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat
     2941 ggtgacaatc ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga
     3001 aattcccttt tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga
     3061 aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg
     3121 gtaaaaatcc gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat
     3181 aatagacggt tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga
     3241 ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct
     3301 ctccttaact aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa
     3361 ttcctgcgta attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt
     3421 ttctaatggc agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg
     3481 cttttacaaa tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga
     3541 tcaataattc aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag
     3601 taatgacttc tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc
     3661 cctttcccaa tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg
     3721 ttccttttac cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat
     3781 cagaagtatc gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta
     3841 cacacgtaat aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg
     3901 ctgctaagcc aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac
     3961 cagcaattgc accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc
     4021 catagagtga aggctcagta attcccatta aagcagtgat cccagtggac acagctagct
     4081 gcttcaactc tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa
     4141 tattagaagc cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca
     4201 tagtagcgat tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac
     4261 cacccatcaa aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg
     4321 ctataagatc gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca
     4381 taattaaaaa taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat
     4441 ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc
     4501 ccgcataatc tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa
     4561 aaagctgtga aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt
     4621 tgacatcaaa ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg
     4681 catcagcaat agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca
     4741 aagtcaagac ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg
     4801 gtgtaaaggt ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg
     4861 aagaggggcc ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt
     4921 ggacttcatt gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac
     4981 caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca
     5041 gtctggtggc acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata
     5101 catttcttcc tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc
     5161 aagtcaaacc acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata
     5221 atcagtaaca tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc
     5281 gcaatcaaca gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca
     5341 gttagatcac atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta
     5401 atattaacta taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg
     5461 aaagcatcaa aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa
     5521 attgatgaat ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa
     5581 tagtgatact tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga
     5641 gatataaagg tatcgataga atgatttggg ctagtattct taaaaattaa ctagcaaatg
     5701 tttaaactga tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa
     5761 taaaaatacc aaacatatcc ataataattt tttctttttc atttctttta tattgttata
     5821 acagtgaata acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta
     5881 gtatttaatt tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc
     5941 tgtttggacg gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg
     6001 tatctccaca tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact
     6061 tccattgtct aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt
     6121 cacatcatca catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg
     6181 tttcgatgac ttctctaatc cagatgtacc tgtcattttt gaaaaaatga tacctataat
     6241 agccaagaca acgataacta taatgataaa cttcttgttc atatttactt tattcaactt
     6301 tttatctcct atctccaaaa taatttgctc ttatccctct tatcagtaca ttgacatccc
     6361 taaaagtaca ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttg
     6421 ttcatcttta tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg
     6481 aataaatgct aacgctacta ccatagaaat ccaaccaata atcaaataca gcttccctgt
     6541 ctcttgtcca tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt
     6601 tactgcgtct gctccagttg tctcatctgt ttcttgtagt gctccgcaaa actggcaata
     6661 cttactttca tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa
     6721 aaacattcaa ttgtttatta gttaactgtt tactttatta aatacatcag aaaacagctc
     6781 attcattggt acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt
     6841 tctccctgtt tgccacgaac ttacacattg ctgtgtaaca ttcatttttt tgctaactcc
     6901 gtttgagaaa gtcctttttc ttttctgtat cggctgagat tacttacttt tttcatgatt
     6961 ctcccctcct gttttattat atacaacaat atgatgtatt tcaactcata aaatataaaa
     7021 aaacttcaaa taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta
     7081 ttaatgaaac agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg
     7141 acacaaaaag aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa
     7201 aattctgtaa cagagcctaa cctcgatagc ttaaccgctc tttctagagc cctaggaacc
     7261 cctgtaagcc attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc
     7321 tatcgttctc ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa
     7381 aggcaagaaa atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac
     7441 tttaacggtg ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt
     7501 taatacaatt gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt
     7561 aaattgataa agaggaacta attcaactcc tgataatatc aattctttta ttacttcatc
     7621 tatccctgct ccttttgcta ggtagactaa aaaactacta cttgctttca cattgtatag
     7681 tctcttaata tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct
     7741 gttttctttt aaagcatttt ccacgctgtt attcccaaca ttacgtagac gttcacaaac
     7801 agtgttagta agttctttct ctgtcattag atatgttcct tgccacgata agccctgtaa
     7861 aacttcatgt gcttcatcta aaatcaattg tttttccttg ctaagttctg atcgggaatt
     7921 ttgaggaaat actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga
     7981 aaaatattga tatgtaaaat gaaatttttc cagaagtgga aggatatctt cattcaattt
     8041 ttgaatcaca gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt
     8101 aaagtttcct aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg
     8161 aacatcttca tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt
     8221 attacattta tctatgtcat aagttttacc atagtaaaaa ctcatttttt tagctaattg
     8281 ttcccccgac aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga
     8341 aagcaagaca tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag
     8401 tttgtctttc attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg
     8461 aaaatgtgta ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact
     8521 gtctttcttt aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact
     8581 ggaatgacaa taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt
     8641 atgatcgttg tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc
     8701 aatggttaac aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca
     8761 gtctttaaac cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc
     8821 ggtttcattg actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt
     8881 catggtaaaa tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat
     8941 cactgcatct gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc
     9001 acacccgggg atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt
     9061 aattgcttct tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat
     9121 acttttttgt atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctat
     9181 gcttaagtat ctactatcat ctttactcaa atctactacc ttccccttct catctttaac
     9241 agtgttatcg acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc
     9301 tttattcttt gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc
     9361 atctcttgta acttctctat agttatctaa acacctatct cttacatcag ttttttcttt
     9421 ttttgtaact ggcttctcta ataatttttc tttatctaca taaattggaa tattaatcat
     9481 tgatgtttcc atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat
     9541 aataaaaaca aactctttat tactatatgt tttagtctta tttgtttact caacctttat
     9601 accgttccat gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta
     9661 taacaggtaa cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat
     9721 attcacttgc ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta
     9781 gcataggctt tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa
     9841 tgctatcaat gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt
     9901 ttctaagaat tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt
     9961 aatcaaacaa aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaaga
    10021 ggtgtcaata tctttgtaaa atttatttac aacacccctt tttcgaggta tactaatttt
    10081 aggaggtgtg gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat
    10141 actcggaaca aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg
    10201 tcttatgatt aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg
    10261 aacctattta ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat
    10321 agaggagtta gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga
    10381 tagcattgta ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca
    10441 acagttccca ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga
    10501 accagagatg caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat
    10561 tagtcttttc aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt
    10621 tgacaataac attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga
    10681 tgaatggcta cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat
    10741 tactgataat ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa
    10801 gaaaaaaatg aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga
    10861 gatgatttca tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg
    10921 tataaaggac acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga
    10981 ggttttagta gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta
    11041 tacggtgtta aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata
    11101 actcatcagc gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat
    11161 aatcaaatac ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt
    11221 caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag
    11281 actgcacaaa ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa
    11341 aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag
    11401 gagcttaata atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc
    11461 ttcagattat tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa
    11521 gacatagaca cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt
    11581 ggtaaagtta taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat
    11641 actgagactc tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa
    11701 cttggataca atactagctc taaagagcaa catgttttta caacagttaa aaacactcta
    11761 tacattccaa acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct
    11821 cgtattacac ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa
    11881 tctgtcaagg tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc
    11941 tatgcacata ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg
    12001 gctcatcagt aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa
    12061 aagctagaac ccttatatat
//
LOCUS       DAZKMZ010000079        12031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000079 DAZKMZ010000000
VERSION     DAZKMZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.079"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..2228)
                     /gene="glyS"
                     /locus_tag="ACG8M5_002208"
     CDS             complement(153..2228)
                     /gene="glyS"
                     /locus_tag="ACG8M5_002208"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0359444.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="ACG8M5_002209"
     CDS             complement(2230..3147)
                     /gene="glyQ"
                     /locus_tag="ACG8M5_002209"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0359445.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3533..4351)
                     /gene="recO"
                     /locus_tag="ACG8M5_002210"
     CDS             complement(3533..4351)
                     /gene="recO"
                     /locus_tag="ACG8M5_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0359446.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4494..5393)
                     /gene="era"
                     /locus_tag="ACG8M5_002211"
     CDS             complement(4494..5393)
                     /gene="era"
                     /locus_tag="ACG8M5_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0359447.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5408..5803)
                     /locus_tag="ACG8M5_002212"
     CDS             complement(5408..5803)
                     /locus_tag="ACG8M5_002212"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0359448.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5787..6263)
                     /gene="ybeY"
                     /locus_tag="ACG8M5_002213"
     CDS             complement(5787..6263)
                     /gene="ybeY"
                     /locus_tag="ACG8M5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0359449.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6292..8484)
                     /locus_tag="ACG8M5_002214"
     CDS             complement(6292..8484)
                     /locus_tag="ACG8M5_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0359450.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8508..9479)
                     /locus_tag="ACG8M5_002215"
     CDS             complement(8508..9479)
                     /locus_tag="ACG8M5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0359451.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10184..11563
                     /gene="brnQ"
                     /locus_tag="ACG8M5_002216"
     CDS             10184..11563
                     /gene="brnQ"
                     /locus_tag="ACG8M5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0359452.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11845..>12031
                     /locus_tag="ACG8M5_002217"
     CDS             11845..>12031
                     /locus_tag="ACG8M5_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0359453.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         3287 a         2561 c         2196 g         3987 t
ORIGIN      
        1 ttatttcctc acttttcatg agcgttttga aaattcgaac aaagaataaa ataacaaaaa
       61 ttattaaaat agaattaata acagctattt ataacaaaaa aaacagagac acagcacgtt
      121 tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg atccaagctt
      181 gctaatgaca aagccatggc agctatttga cttagttgtg ttagtcggtt cttacgtact
      241 gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag tggacgaaga
      301 gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc tttcattgct
      361 tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg gtctacttct
      421 gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa agcttccatt
      481 gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc tgcaaatact
      541 tttgtcagat ctttttgttc cgcattcaaa acagcatcga tgacatcatg acgaatattc
      601 ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacttg ttgttgccct
      661 tctgataaac caatgccgaa acgatccgta tctttactga taacatcgct aatcgtttct
      721 tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgatccgaac gacaccgtat
      781 gcttggcgac gtaaggcata aggatcgttt gacccgcttg gaatcatccc aactgcaaag
      841 aaagaaaaga tgctatctaa tttatctgca atcgctaaaa cagaacctac tgtagactga
      901 ggtagctctc cttcactgga tataggcatg taatgctcac gaatcgctgt cgctacagca
      961 ggtttctcgc ctttcagaag agcatatttt tcgcccatga tcccttgtag ttcaggaaat
     1021 tcccctacca tgttggttac tagatcaaat ttatagattt cagcagctcg ttgcaaatcc
     1081 atcaattctt cctcagatag tcctacttgt tcgccaatga tacgggcaat gacttgtacc
     1141 cgttgcattt tctcatagat actaccgatt ttttcatgga aagtaacagt ttttagtttt
     1201 tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc cgcatcttct
     1261 aaacgagccg tcaatacttt ttgattgcct cgaaccacat cttccaattt tacccgatca
     1321 ccattacgag cagaaataaa atgaggaagc agcatacctt gatcattccg tacctcgaaa
     1381 taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga aagatatttt
     1441 tcttcgaagt cgccagaaaa gacagttgga tattcgacta aattgttgac ttcttctagt
     1501 aaatcttcgt ctagatcaat aacccagtga tttttttctg ctaattcgtt tgcttgtgta
     1561 acgatcattt cttgtctttt ttgcggagac gcaatgacga attgttcatt caatttttct
     1621 tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg acctcgagag
     1681 gctctgcctg ttttgacatc caagatttcg aatgggatta cttcgtcatc taacaatgcc
     1741 acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg catcgttaca
     1801 gggaaagtca aacttttgat cacccctgct aagcctgtca atacttcttt tgcttcctta
     1861 ccatgagtgt atttcgttac atagacatat tccacacctt ttaattctct aaaagtgata
     1921 gcgtctgtcg tcagaccttg tccgcggaca aatccttccg ctgctttcgt ccagtttcca
     1981 tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact gtccgcttgt
     2041 cgttctggaa tatttgttac tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc
     2101 gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc taattgtttc
     2161 atgcttggtg tgacaacatg ggcaggcatt tcttccagcc cgatttcaag taataagtct
     2221 tttgccatct tattcagcct ccttcacggt ttctggattt gctgtgtttt tattcaacaa
     2281 agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac gagccatgtt
     2341 gcggatgcga gaaagataac cggcacgctc tgttacggat actgcaccgc gggcgtctaa
     2401 taagttgaat gtatggctac atttcaagat ataatcatac gccggatgaa caagattttc
     2461 atcgatacag cgtttagctt ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg
     2521 gtcgcttact tcaaaagaat atttggaatg ttcatattct ggttgtttga agatctcgcc
     2581 atatttcacg ccatcagccc attctaaatc atagacactt tcaacttctt ggatataaga
     2641 agcaagacgt tctaatccat aagtgatctc agaagttact ggtttacaag gcagtccacc
     2701 aacttgctgg aagtacgtga attgtgtgat ttccattccg tcaagccata cttcccaacc
     2761 cacaccggca cagcccatgg atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg
     2821 ttctaatgga tcgattccta aaaggcgcaa gctttctaaa taaagctctt gaatattctc
     2881 aggtgaaggt ttcatcacca cttggaattg atgatgctga tacaaacggt ttgggttttc
     2941 accataacgt ccatcagctg gtcggcgaga tggctctacg taagcagcat tccatggttc
     3001 tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta cctgctcctt tttctgtgtc
     3061 ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat
     3121 atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaatttta aagcgttgct
     3181 gtcaaataag aatacaggaa agaaaacgac aaaaaatccc tatgtctgat tacagacata
     3241 gggacgaata attcgcggtt ccaccctact tccgaataaa gattcggcag cttcatttat
     3301 cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg ctcccactct cccgaactcg
     3361 ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg cagttttctt
     3421 gtttctataa tagatcctat cactgaacga tctatttaat ctatggttaa taatagacgg
     3481 aactccctgt gtttgtcaaa tggttttgat tttttgttgt atgttttatt tactattctt
     3541 ctttttcagg tgtctctttt tctttttcag acatgtcttt ctctgacgtc tttaatacgt
     3601 gtgcccaaga cttcatttga ttgatgaact ttttgctttt caaatggata ccgacatatt
     3661 catcatacag ctggtcgatg agttcacgga ttgcttgttt ggtttcttct ttcagatgga
     3721 ttccactaat tttatcataa gaaatagctg aaaacatccg aacgaaataa acagctcgag
     3781 gatctgcatg ataacggtga tcgtccatcg accaatgtct ttcgcatatc acaccaccat
     3841 atttggaaga atagtcaaat tttccttttg tttccccgca tacgacgcag tgtgtccaga
     3901 ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa aatattcgtg atgatttctg
     3961 catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt aaactggaac aaatgcgggt
     4021 cgtactgatg atcttcgatt gccgcatcta ctagattcaa taaatatgtg ccatagccgc
     4081 tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac atctttgaca ctgcttaaaa
     4141 atgacaaacc ttcacttcga aaattcccga tataaactgc ttcagtaaaa gggagcaaag
     4201 ccggagctaa gggctgattt tgccgatgga tctctttgat ataaaacatc attttgccag
     4261 aagattctgt aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa
     4321 tcaagccttt tgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa
     4381 aagcagattc tgccgatcca gatcatttat gttgcgatct tccgcagctt ttctcaacac
     4441 gcggattttt attcagttta tttctatacc taatttacaa gaagctcaga atatcagtat
     4501 tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca gtctttttga
     4561 actttgaccc aaagttctag aaagactttg tctcctaaaa gattttcgat atcttttctt
     4621 gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat ccctttttgg
     4681 ctatctcttt cgacaataat cgtagcctgg atatggattt tatcattctc gtttcgcttc
     4741 atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact
     4801 ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac ttgatcatct
     4861 ggaaaatatt gtgggccctc tggcatttca ctgactaata catccatcaa acgttcgacg
     4921 ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa aaggcatctg cgttgaataa
     4981 tcttcgatga ttcccaacaa ctcgtctgga tggattttgt cgattttatt gataatcaga
     5041 taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt
     5101 ttttgatcag cactaatcat aaataagacc gcgtccactt ctttcaacgc actgtaagct
     5161 gcttctacca taaaatctcc taaacgatgc tttggtttat ggattcctgg tgtatcaata
     5221 aaaatcaact gagcttctgg tgtagtgtat actccttgga ttttatttct agttgtctgt
     5281 gccttgtcac tcatgatggc aattttttga cccacgattc gattcaataa tgtggatttc
     5341 cctacatttg ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatatgtt
     5401 ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc aacccaacga
     5461 tgacggtaaa acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc
     5521 caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac attttcaaaa acagtattga
     5581 tgatctctac tatccatacg agaaaaacag ctaaaagcag ccataaccat tctatccgat
     5641 caagttggaa aatcacgccc ataaacaaag cgaggattcc gaacgcaaca tgtttcctca
     5701 tgttgcgttc ttcctcaaaa acagttttga ctccttggac ggcaaattcc acggacatca
     5761 ggaaatgttt gtttttctct acgtttttat cttttaagtc cataggcatc taagatctct
     5821 ttctgtaaac caaacatttc tttctcatcc tctggtgtca tatggtcata cccgttgata
     5881 tgcaagaatc catgaaccgc taaaaagcca agttcccgat catacgaatg gccgtaagct
     5941 tctgcttgct ctgcagcacg ttcagtagag atcatgatgt ctcccagtac acgaggcaat
     6001 ggattctctc catcttcaaa aatcactggg atttcgtctt cgccctcttc ttccaaggca
     6061 aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt
     6121 gctgcattgt ccatgaaagt aacagacatt tctgtttctt ctggcaactc taaatatgat
     6181 gctgcaaatt gcagcaagtc gtcgatttct ttcattttct cttctgagac ttggtttgtt
     6241 tcatccatga aggtaatatc cattatacat cgcttctttc tattcttctt gctattcttc
     6301 ttgttcttct tttatcttct caacttccga tttcatttta ggatatttga tccgagaatg
     6361 gaaggtactg cttaaaccgc tgatcaatga tttttctact ttacggattt cttttaacgt
     6421 caatccactg tcgtccaatt gtccatcgga aatacgctct tcaataagat tatggacaaa
     6481 actttcgatt ttttcgctcg ttggatggtc cattgcccgt actgccgctt cacaactgtc
     6541 agctatattg acgattcctg cttcacggct ttgaggtttt ggtccaggat aacggaattg
     6601 ctcttccgta acttccgggt tgcgttcctt tgccttaacg tagaaataac gcatcagcgt
     6661 ggttccatga tgctgtctac aaatatcgat caccatttgc ggcatattat aatcttccaa
     6721 tattttggca ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6781 gaaattgtgc ggattttccg ctcctgaagg caagttctca acgaaaaagc tggcgtgttt
     6841 gatcttgccg atatcatgat aatagcaagc tactcgagtt agtaaagaac gtccaccgat
     6901 ttctgctaca gcattagcac ttagattggc taccatcatg ctatggtgat acgtacctgg
     6961 tgcttcttcc agcaactgtt tcaataaagg atgattggga ttgctgagtt catttaatac
     7021 gatcacacta tcatcattga ccatcaattc aatatagggg tgcagaccta tcgtcagcaa
     7081 gaatgaaaac agtgcacctg tgaatccgca gactagcgtt aaccaagtcg ttgcatcccc
     7141 aaagctcatt ccttggtaga taatcaaaat gacatccatc atcacaggaa aaataatgac
     7201 ccacatagca gcggataacc cttgttctga gatccgtttg cgtctgacga tcgttgccat
     7261 gagaccagaa aatacatagg acatcaaaat aactgtcaga aagttcgtgc caatcgaatc
     7321 ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca gccatgatcc ctgctcttcg
     7381 attcaagaaa aagcttaaaa caagtggagc aaaagccgcg gggtaaaaca gcggaatata
     7441 agcagcttgt tctgtttgga agagttggaa aaatttcatt agtacgacgc ttactgacat
     7501 tgccgtcacg tagaaaagta cgtattttgt tctttctcct gcattttgga attgaagtga
     7561 attataaatc agtacaccga tttgaagcaa aacagctaaa accatggcaa tgagtgggaa
     7621 aatagaagtc gtttgattcg tcaatcctag taattccaat tttttcatcg ctgaagcatc
     7681 aatttgattt ccttcgcgaa caataatttc tccttggaag atcatcactg gttgtacagc
     7741 ttctcgtgca gattgtttta attcttctgt ctttttctca ttcaatacat cattaactac
     7801 gatgccttga tccactaagt agcgaatcat ttgttgctgc tctgttgtga cattcaaata
     7861 ttgaatctga tcttctgctt cttgtttaaa agcttccaat tcgctttcac gaacttgttt
     7921 actcatctgt tcgtcaatga gcttcaaact ttcatcccta actgtgtgaa tttccgtttc
     7981 agtcatttca aatatcgtcg aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt
     8041 ttctgcattc acgttttcaa aatttttctt cagtgtagca atccgctctt ctgtagtagg
     8101 tttcgggata ttttcatctt ccttcgcctt atctaccttg ccattataag atttgttgat
     8161 ggtatcattc gtcttatcga tcaattcgaa taattgcttg atccgattgt tctggtcatc
     8221 tgccagatct ttttgataag tatattctgg agtcactgct tctgctgcca gttttcttct
     8281 ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca cggatactct cttctgctac
     8341 ttggccttct ctgaaatcaa tggccttttg tttgacacta ctaaatgtaa cgccgcagac
     8401 aatgataaaa aacaagaaca gaaggaaagg caatgctgcc tttccgagtt tttcttgtag
     8461 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatga
     8521 agatccgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8581 cttgcttcaa aattaacaaa atcgattttt ttgatttgct ttaatgttcg ttctgcatga
     8641 acaagaccac ttgtcgtccc acgaggcaag tctatctgac ttgtatcccc attgacaatc
     8701 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8761 tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa
     8821 ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8881 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt cagatccccg
     8941 ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc
     9001 acttcgcctt tttttagggc agcaattgct aatactacgg caagaaacgt tttccctgtt
     9061 ccggctggcc ctacaccaaa tacgatgtca tgtttggcta ccgattcaac gtatttcttt
     9121 tgaccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9181 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9241 tctggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9301 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9361 atggttgttt ccgttgtttc ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9421 atgctgacat catcagcagc tgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9481 aatagacatc cccttttttt cgattcattc tttcctattt taccatactc agaagaaact
     9541 atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac catacgtacc aagaaacgtt
     9601 aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt
     9661 aagttatatg aaagtggaga acaaaatatt tcgtttcatt caacaagtat tctctaggta
     9721 tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa
     9781 acaggtgtat tattacgtca acaaataaac atacagcaaa gcgtcgagga aaacagtagg
     9841 ctattcaacg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9901 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
     9961 aggctagcgg ataactttat caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
    10021 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gctttttttt
    10081 attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat tgctgcaacg
    10141 tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag aaacaaaaac
    10201 ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc
    10261 aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact gggtcagtgc
    10321 tggcgtagga tttgctatct caggtgctct tttcccatta ctagctatct tagctgtagt
    10381 agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat ggtatgctgc
    10441 cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac cacggacagc
    10501 tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata attcattagg
    10561 tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg tccatccaac
    10621 acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat tattagcaat
    10681 catttttgtc gtttctttat tccatccaat gggaaatcta aatcaatctg ctgcagatac
    10741 ttatcaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg ttgatgcagt
    10801 agctttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga
    10861 taaaaaagtc acagaattaa cggcaaaagc tggtagcctg agcgttgcgc tagaagtcat
    10921 gatctatttt ggacttgttc ttctcggtgc gatgagtttg aataagatgt ctttatcaga
    10981 aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact tcggcgctag
    11041 cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac tcgttgtttc
    11101 ttttgcgcaa gatttccata aactgttccc taaagtcagt tatatgacat ggctgcggtt
    11161 aacgactttc gtctcttttg tcgttgcaaa tgctggttta gacaatatca tccaatggtc
    11221 tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgatcttgc tttccttaac
    11281 agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt ttgctgctgt
    11341 tcctgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac aaagagttgt
    11401 agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattag gacttggctg
    11461 gatggtccca acgttacttg gttacgctgg cagcctactg ttttattacg cctatcgcct
    11521 atcagggtat aaacaagaag caaatgaact accagaagaa taaccaaatc aaacacaatt
    11581 taacataaga aatattaaac aggaaaaaac cagctagatg aaagaatcct attctttcgt
    11641 ctagctattt tttagttctc ttttttacgc gcacaaaaaa actgcggaca atgattgtcc
    11701 gcagtttaaa tgggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt
    11761 ttcttttttc taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa
    11821 aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat
    11881 attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac
    11941 gaaacatgtt ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgt
    12001 cactatcaaa ataagcaaac gatcattaaa t
//
LOCUS       DAZKMZ010000080        11285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000080 DAZKMZ010000000
VERSION     DAZKMZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.081"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8M5_002218"
     CDS             complement(33..1076)
                     /locus_tag="ACG8M5_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0359454.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG8M5_002219"
     CDS             complement(1237..1920)
                     /locus_tag="ACG8M5_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0359455.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG8M5_002220"
     CDS             2115..3044
                     /locus_tag="ACG8M5_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0359456.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8M5_002221"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8M5_002221"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359457.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8M5_002222"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8M5_002222"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0359458.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8M5_002223"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8M5_002223"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0359459.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG8M5_002224"
     CDS             5429..6232
                     /locus_tag="ACG8M5_002224"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0359460.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG8M5_002225"
     CDS             6316..7863
                     /locus_tag="ACG8M5_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0359461.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG8M5_002226"
     CDS             complement(8160..8357)
                     /locus_tag="ACG8M5_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359462.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG8M5_002227"
     CDS             complement(8433..8633)
                     /locus_tag="ACG8M5_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0359463.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG8M5_002228"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG8M5_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG8M5_002229"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG8M5_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0359464.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3291 a         2205 c         2246 g         3543 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaa
//
LOCUS       DAZKMZ010000081        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000081 DAZKMZ010000000
VERSION     DAZKMZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.082"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M5_002230"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M5_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0359465.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M5_002231"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0359466.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8M5_002232"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8M5_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0359467.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M5_002233"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M5_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0359468.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M5_002234"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0359469.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8M5_002235"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8M5_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0359470.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8M5_002236"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8M5_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0359471.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8M5_002237"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8M5_002237"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0359472.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8M5_002238"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8M5_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359473.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8M5_002239"
     CDS             9295..9939
                     /locus_tag="ACG8M5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0359474.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8M5_002240"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8M5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359475.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8M5_002241"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8M5_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359476.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKMZ010000082        10768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000082 DAZKMZ010000000
VERSION     DAZKMZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.083"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..560)
                     /locus_tag="ACG8M5_002242"
     CDS             complement(<1..560)
                     /locus_tag="ACG8M5_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0359477.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHN"
     gene            complement(1128..2195)
                     /locus_tag="ACG8M5_002243"
     CDS             complement(1128..2195)
                     /locus_tag="ACG8M5_002243"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0359478.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(2215..2694)
                     /gene="pssE"
                     /locus_tag="ACG8M5_002244"
     CDS             complement(2215..2694)
                     /gene="pssE"
                     /locus_tag="ACG8M5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0359479.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(2711..3172)
                     /gene="pssD"
                     /locus_tag="ACG8M5_002245"
     CDS             complement(2711..3172)
                     /gene="pssD"
                     /locus_tag="ACG8M5_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0359480.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(3186..4436)
                     /locus_tag="ACG8M5_002246"
     CDS             complement(3186..4436)
                     /locus_tag="ACG8M5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0359481.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(4814..6265)
                     /locus_tag="ACG8M5_002247"
     CDS             complement(4814..6265)
                     /locus_tag="ACG8M5_002247"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0359482.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(6586..7350)
                     /locus_tag="ACG8M5_002248"
     CDS             complement(6586..7350)
                     /locus_tag="ACG8M5_002248"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0359483.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7378..8076)
                     /locus_tag="ACG8M5_002249"
     CDS             complement(7378..8076)
                     /locus_tag="ACG8M5_002249"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0359484.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(8088..8867)
                     /locus_tag="ACG8M5_002250"
     CDS             complement(8088..8867)
                     /locus_tag="ACG8M5_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0359485.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(8883..9827)
                     /locus_tag="ACG8M5_002251"
     CDS             complement(8883..9827)
                     /locus_tag="ACG8M5_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0359486.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(9873..10652)
                     /locus_tag="ACG8M5_002252"
     CDS             complement(9873..10652)
                     /locus_tag="ACG8M5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359487.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         3141 a         2232 c         1590 g         3805 t
ORIGIN      
        1 atattgtgct gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat
       61 ataaaatcga ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt
      121 atagcgttat ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta
      181 tcatcataat attcataata tccagcataa catgtgtctg cattactcct ttttatacca
      241 tttaacattg cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca
      301 tattttcctt ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt
      361 ttatgaataa tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat
      421 gaatccttag atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac
      481 actgattcca aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta
      541 attagatcac tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg
      601 acggcatcaa cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg
      661 aatgacatac tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata
      721 atatgatcct cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac
      781 aatattgcaa ttttatttct taatatgatt atttcactca ttcattactg ccacatatac
      841 acaacttata accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt
      901 catcatccag aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat
      961 gtaccgtgct tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt
     1021 cttacacgga taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac
     1081 ttaataagtt cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc
     1141 cctttttgat acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt
     1201 gccaaatcct tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt
     1261 attgcagatg tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca
     1321 cgtactgctg aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc
     1381 gcctctagaa tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat
     1441 cttctatagc actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat
     1501 tgcaaattat attcttctat caattctttg agcgatattt cttcatcacc tgctccagca
     1561 atctcaaaaa taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc
     1621 aaatcgtgta caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt
     1681 gaattccact ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta
     1741 caatttggct caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa
     1801 tcagactctc ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac
     1861 cattttttaa attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac
     1921 cacttattca gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata
     1981 atattaggtt tactcaataa agagatacct ataattttag caaatgcata cataaaatat
     2041 aaagatttaa atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc
     2101 tttttttccc aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc
     2161 gagctgtcat taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat
     2221 tttttctatt aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt
     2281 aaacatccga actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg
     2341 atagataata ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg
     2401 ctcaccatac ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc
     2461 aattatagca cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt
     2521 aaactcatcc ctatttagaa atgctttata ggaataattt agtggtttat aatcactata
     2581 accactctgt gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc
     2641 ttttaacaat ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt
     2701 ctcctattat ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag
     2761 tttcccactg aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag
     2821 ggctggttac ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta
     2881 ttaagcagat agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct
     2941 ttatatatat aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt
     3001 cttttctatt tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt
     3061 ctgtctgttc tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta
     3121 actgttcata atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct
     3181 ctctatcagt cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag
     3241 caggtatcat atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg
     3301 ttgataacct tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc
     3361 acagtcgcac ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa
     3421 ttatcactgt tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg
     3481 ttgacatcat actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga
     3541 tctgcgatga aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca
     3601 ggaacatctg catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca
     3661 tatccaaagg atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg
     3721 attatctgct gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag
     3781 ctgactcgca gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat
     3841 cccataaaaa gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa
     3901 acatgagcag catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat
     3961 ggatacagat tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg
     4021 ttcttactcc ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg
     4081 atgaccatga tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca
     4141 gaagtatcga aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa
     4201 tctgcggatt tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct
     4261 gtaagatact tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca
     4321 atcttttcag ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg
     4381 atactcaaac caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata
     4441 tctcctcttt cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa
     4501 aataacacag cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca
     4561 tttataaacc acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa
     4621 taacaagata cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata
     4681 aatcttcatt ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat
     4741 tccaaatctt tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta
     4801 taacactttc gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata
     4861 aaattttaaa atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta
     4921 cttcttcaaa atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg
     4981 gcttaatagc aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc
     5041 atggtcgaaa tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt
     5101 catctaaaga cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa
     5161 gtattttatt tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca
     5221 tcttattttc tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa
     5281 acttatacat cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg
     5341 ggcctggtga attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa
     5401 tacatccaac aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg
     5461 tcataacatt gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt
     5521 ttcccaaagc actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag
     5581 agcatcgttc aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt
     5641 catctaccca tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca
     5701 ctccctcaac atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat
     5761 acattttata gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg
     5821 taactataat aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc
     5881 ataagaggcg agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga
     5941 ctctagaata atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca
     6001 acataatcgc ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac
     6061 ctctatatgc ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca
     6121 tgttttgata cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata
     6181 gataaaaagc aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc
     6241 atccccttga acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt
     6301 gtattacttg ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc
     6361 ttgtggatat tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt
     6421 tttttactca tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt
     6481 ctgcttactc aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg
     6541 atccccttgt gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa
     6601 cttcttcttt tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc
     6661 ctttgcgact tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc
     6721 atagcactct tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat
     6781 ctgcacgaga ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac
     6841 cccaacataa cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa
     6901 atgatttggg tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt
     6961 tcctagttct aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg
     7021 aaattcgatc agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat
     7081 gtcttcgatg agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa
     7141 ctgtcttttc tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc
     7201 attgctgtat tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg
     7261 aatcgctttt tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc
     7321 tggtaggata tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta
     7381 atttccgtag taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa
     7441 gactcgtgct tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga
     7501 catattttca cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc
     7561 tgttacggct actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc
     7621 ggataggatc tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg
     7681 tccactaggc aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact
     7741 tcctgatgaa ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata
     7801 gacgacgggt ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa
     7861 aacgactgca atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac
     7921 gacaatcgtt ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt
     7981 acgatattgt tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg
     8041 agtttgtgct gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag
     8101 cggcttctgc gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt
     8161 ccttttttac ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag
     8221 attgggaatc ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat
     8281 tccaataaga atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa
     8341 ttcaatttat tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag
     8401 attttatcaa cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta
     8461 gcgatctgtt gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa
     8521 ttttctgcct gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg
     8581 tcttctgttt ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct
     8641 ttatacgtat tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat
     8701 tgtggcagtg tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat
     8761 gttacgattc ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt
     8821 ttgcgtaatg tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt
     8881 tcctatttat tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct
     8941 tgatcactga ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga
     9001 tcttgcttaa tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca
     9061 aagttcatgt ctgttttcat attcgactct agagcattca aaacggattg atagttgctg
     9121 acaccgttta gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct
     9181 tgtctaccgt aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga
     9241 tccccgttca atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg
     9301 tcattattga cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg
     9361 atagagacat agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg
     9421 gcagctccgc cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg
     9481 tcgacatacg tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact
     9541 gtggcaacca tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca
     9601 atccccatca atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt
     9661 tctccttggg ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga
     9721 tataattttg ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata
     9781 atcaaaaata tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt
     9841 tgctaacctt gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt
     9901 tgctgagcaa cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc
     9961 tgctctattt gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga
    10021 ccgtctttca gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta
    10081 tcagcgaatc ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc
    10141 atattttcac gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca
    10201 tccacagctt gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg
    10261 atcttcagtt ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg
    10321 atcgcagcag cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt
    10381 ttgatagaag atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt
    10441 aggaaaattt tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg
    10501 gtgtcttttt gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc
    10561 tgattattga gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca
    10621 gtagccaata acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga
    10681 taagtaaaga tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt
    10741 aaactaatat attcatagaa atatttat
//
LOCUS       DAZKMZ010000083        10767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000083 DAZKMZ010000000
VERSION     DAZKMZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.084"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1820)
                     /gene="glmS"
                     /locus_tag="ACG8M5_002253"
     CDS             complement(15..1820)
                     /gene="glmS"
                     /locus_tag="ACG8M5_002253"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0359488.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2229..2519
                     /locus_tag="ACG8M5_002254"
     CDS             2229..2519
                     /locus_tag="ACG8M5_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359489.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2532..3557
                     /gene="sppA"
                     /locus_tag="ACG8M5_002255"
     CDS             2532..3557
                     /gene="sppA"
                     /locus_tag="ACG8M5_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0359490.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3560..4219
                     /locus_tag="ACG8M5_002256"
     CDS             3560..4219
                     /locus_tag="ACG8M5_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0359491.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4376..5308
                     /locus_tag="ACG8M5_002257"
     CDS             4376..5308
                     /locus_tag="ACG8M5_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359492.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5462..6751
                     /gene="tig"
                     /locus_tag="ACG8M5_002258"
     CDS             5462..6751
                     /gene="tig"
                     /locus_tag="ACG8M5_002258"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0359493.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6998..8248
                     /gene="clpX"
                     /locus_tag="ACG8M5_002259"
     CDS             6998..8248
                     /gene="clpX"
                     /locus_tag="ACG8M5_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0359494.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8331..8918
                     /gene="yihA"
                     /locus_tag="ACG8M5_002260"
     CDS             8331..8918
                     /gene="yihA"
                     /locus_tag="ACG8M5_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0359495.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8992..9147)
                     /locus_tag="ACG8M5_002261"
     CDS             complement(8992..9147)
                     /locus_tag="ACG8M5_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0359496.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9283..10611
                     /locus_tag="ACG8M5_002262"
     CDS             9283..10611
                     /locus_tag="ACG8M5_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359497.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         3560 a         1866 c         2229 g         3112 t
ORIGIN      
        1 tccttgttct tttattattc tactgtcact gatttcgcta aattacgtgg tttgtcaacg
       61 tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt gagtcggtac gactgtggtt
      121 agagtagtta gataagggtg gacagttggt agcaccagct ggtcactttc cttttctaat
      181 ccctctgaga caataactag tgttttagcg ccacggcttt cgacttcttt caggtttcct
      241 cgagtatgcg caccagtcac ttcttcactg atgattccga tcactggtgt tccttcttcg
      301 atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa aaccttctgc ctggatataa
      361 ctgatttctt tcaatttcaa agcagcttca agagaaacgt tgtagtcagc accacgtcca
      421 atgtaaaagg catttcttgt atcgctgaga tatacttctg ccaactcttc taaataatct
      481 ttttgggcga tgaccgattc catcgcatta gcaactaaac tcaattcatg agtgatatcg
      541 aatacaagag aagtttcaaa ttctttttta tcaccgatag ctttggctaa taaagttagt
      601 acagctattt gtgcagtata agctttcgtt gatgctactg cgatttcggg tccagcatgc
      661 aatagcagtg taaaggatgc ttcccgcgaa agagtagaac cagcaacatt tgtaatcgta
      721 agtgaagggt agccgagacg gttgattttt accagcactt ggcggctgtc tgctgtttcg
      781 ccactttgac tcaagaaaat aaagaatggt ttttttgaaa gaagaggtgt gttatagcca
      841 aattcactgg ctaaatgaac ctcaacagga atctgtgtca atttttccaa agtctgttta
      901 cctgctaacc ccgcatggta actagttccg caagcaacga tatagattcg atcgctatca
      961 gacaatgtat cgaccagctt ttgatcaaga gagacacgat tgtcttctgt tacgtatttt
     1021 tgagcgatac gacgcatgat aataggctgt tcgtcgattt ctttcagcat ataaaaagga
     1081 taggtacctt tctcgatatc tgaagcatct acttgtgctt cgaatgaaga acgttctacg
     1141 atgtcacctg caaaattttg aataataact ttttctgatg tgacggtaac catctcttca
     1201 tcttcaatct caacgaattc cttcgtatga gcaagcatag ccattgcatc gctggcaatc
     1261 acattgaatc catcgccgag tccgattaat agtggacttt tatttttagc gacgtaaata
     1321 acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag aacctttgat gacccgcaaa
     1381 gcttttaaaa acgcctcttg cgttgtagta tccttcgcga aagtttcaat tagatgagcg
     1441 ataatctcag tatcggtctc accaataaag tggtcatttg ctaaataagc aattttaagt
     1501 tcttcaaagt tttcgattac gccgttgtga acgagcacga agcgtccact ttgggaagta
     1561 tgtggatggg cgttttcttc agaaggttgc ccgtgagttg cccatcgtgt atgaccaata
     1621 cctatatttc ctgtagtatc aatatttatt ttgtttttca aattttgaat tctgccttgt
     1681 gctttgacca gataatcagt tgtgccatct gagacaaaga taccagctga atcataacca
     1741 cggtattcta atttttctag accgtcaatc aatcctggtg ttacattttt taatccaatc
     1801 attccaacaa ttccgcacat aaaaaacatc ctactttcta taatgaattc gtatgaatgc
     1861 ttccagctgc aggaaaatcc ttttgtttta tctttgcaga cattttttgt ggattttctg
     1921 tagagtgcag tccctagggc catccgccga atctttcgat aaaccctctc ctcgtcatct
     1981 gtttacacag acctggcgct atcctaaaag gtataactat cctccttttt gttttttatc
     2041 tggaaacatt taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa
     2101 aataaattgg tatatatcaa attctttgtc aaatgtcctt tttatgtcaa aaaataatgg
     2161 gtttttatct atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag
     2221 gagtaaaaat ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt
     2281 ccaaattcga agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct
     2341 gggatagcat ccaagaaatc gcacctgatg ccttggaagt tgctcaagct cgtgagttta
     2401 cggtagaaga tgaattaaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag
     2461 agcggttcga cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga
     2521 aaggagaaag aatgaataaa agacgttggg cagcagttct gattgctgca ggattatttg
     2581 tggtttcgtt agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat
     2641 tgaacagttg gttatatggg gatgaagaac tagctccaaa tgtgttggaa gagggagaca
     2701 gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag
     2761 gtttatttgc ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg
     2821 aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct
     2881 acgaaagtgc ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa
     2941 tgtatgtcag catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag
     3001 ataaaatctt tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat
     3061 tgaattattc aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag
     3121 cgttgaaaga tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc
     3181 aagcatatat cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata
     3241 aatctgaaga agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa
     3301 aagaagtagg tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatacgaa
     3361 aggaacaaca gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg
     3421 ctagtacatg gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta
     3481 gtcagatctt atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt
     3541 attacggagg tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga
     3601 taagcaacag cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc
     3661 ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa
     3721 gtatacggac acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc
     3781 aggttttttt atccgaatgt ttgccttctt ggtcgatctt ttgtgtatcg gagctattac
     3841 gcgtttaaca cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct
     3901 gagtctctac ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa
     3961 attcaatcat ggccaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga
     4021 tgaagaagaa cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct
     4081 aaaatttcct ttgctgatgt tagggtatct tccagcagtc tttaatcaga aaaagcaaca
     4141 tctgggcgat tttttcacta atacaagtgt tgttacgatc aatctgatca aagcgttcaa
     4201 ccaggaagtc aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa
     4261 agtcagatct aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt
     4321 ttttgttttg ctactattga acatgagctg aatatgagca agaggaggac actgcgtgaa
     4381 gccgattgat tttccagaaa aatatgagaa tttaatgcga gtggcacaac aagcactagc
     4441 aaaccagcaa tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag
     4501 ctttgaggct aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc
     4561 attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt
     4621 ttattttgac ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag
     4681 tacagatttt tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga
     4741 attatccgga caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact
     4801 tccttcgttg gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta
     4861 tcatgagttt atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc
     4921 aagggcaaaa ttactggaga cacttaggca attgaacgaa tgccatcaag ttttctattt
     4981 gacgattgaa gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca
     5041 ggcaagttac cagcaattat gtgtattttc agatcgttac gggaatgaag atgcgctgct
     5101 tagttccgtg ttaaaagaag aattcacgtt gcaatctgca attgtttatc ctgtctatga
     5161 tacatacatc gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa
     5221 aacgatgtat gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat
     5281 tttgcagcaa atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg
     5341 aaaaacaagg attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt
     5401 aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta
     5461 aatgactgca acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc
     5521 gcaagacgaa atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa
     5581 cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga
     5641 agaagcttta tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt
     5701 caaagaagct ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa
     5761 aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca
     5821 atataaagat ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc
     5881 acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc
     5941 tgaaaatggc gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga
     6001 tggcggaaaa ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt
     6061 tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc
     6121 agaagattat caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca
     6181 tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga
     6241 cgaagtatca acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa
     6301 agaaaaagca gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc
     6361 agaaatcgtt gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga
     6421 attcttgaac aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg
     6481 ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac
     6541 aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat
     6601 caatgcagaa atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg
     6661 cgtgttgaca gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat
     6721 cacaggtaca gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa
     6781 gctacttcta gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt
     6841 ccttattttt tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa
     6901 cactttttct ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt
     6961 ttcttgcata ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa
     7021 tgagaccgta cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc
     7081 tggtccaggg gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga
     7141 agaattttat gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct
     7201 tgaagtattg aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc
     7261 cgtatacaat cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga
     7321 actgcaaaaa agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc
     7381 acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac
     7441 agaagctggt tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc
     7501 tgattacaat gtggaacttg cagaaaaagg gatcatctat attgatgaga tcgataaaat
     7561 tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca
     7621 agccttgctg aaaattctag aaggaactgt tgcaagtgtg ccacctcagg gcggacgtaa
     7681 acatccacat caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg
     7741 tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt
     7801 tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga
     7861 agatttacta aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc
     7921 tttagagaaa ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct
     7981 agtcaaacaa tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga
     8041 agcgttgaga gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg
     8101 ttccatcatt gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat
     8161 cgaaaaagtg attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta
     8221 taaagattca gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc
     8281 ttttgcgaca gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc
     8341 atcaagctga aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc
     8401 cagaaatagc tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga
     8461 ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt
     8521 tttatttgat cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag
     8581 tatccaaaac tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag
     8641 accagctacg tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata
     8701 tccagatgta tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg
     8761 cagataagat ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg
     8821 attttgatcg caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag
     8881 aagcgtgggc agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca
     8941 gtcaaactct ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg
     9001 tttcttgtct tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc
     9061 ttttggtgca actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt
     9121 atctgtacgt tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc
     9181 tatcatgtaa tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcatt
     9241 ggttataatt aaattataaa aagatttgaa agaggtgaga aaatgattgg cgaagataaa
     9301 ttattgatga ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc
     9361 gattgggcta ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa
     9421 caagcggatc cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga
     9481 gacgaaagtt ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat
     9541 aatcaattat tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg
     9601 attcgagact tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct
     9661 tatagcgagt ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa
     9721 aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat
     9781 tgcaaccaat ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg
     9841 gaaatgtatt atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc
     9901 caacaagtag aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga
     9961 gaccccttta actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag
    10021 cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt
    10081 gaatacattt acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa
    10141 cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat ccaacaagga
    10201 gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt
    10261 gacgagaatc gatcacgcat gatgtgctat aaagttatcg atccgactgt tgctttggat
    10321 aatggaatcg aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat
    10381 gaaaaatatc aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca
    10441 gaattaccgt taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag
    10501 aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa
    10561 tttttagatt accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa
    10621 aatggataaa caactctatt atcgaataat ggttccggca tcattattat ccgtctgtct
    10681 cttttttgtt cctaattttc ctattcgatc agtcatcggt tttttggtca tgtttggcgg
    10741 gatgggactt tattattatc ttcttca
//
LOCUS       DAZKMZ010000084        10642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000084 DAZKMZ010000000
VERSION     DAZKMZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.085"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8M5_002263"
     CDS             34..657
                     /locus_tag="ACG8M5_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359498.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8M5_002264"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8M5_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0359499.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8M5_002265"
     CDS             2153..3034
                     /locus_tag="ACG8M5_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359500.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8M5_002266"
     CDS             3037..3807
                     /locus_tag="ACG8M5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359501.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8M5_002267"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8M5_002267"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0359502.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8M5_002268"
     CDS             5118..6281
                     /locus_tag="ACG8M5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0359503.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8M5_002269"
     CDS             6398..6808
                     /locus_tag="ACG8M5_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0359504.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8M5_002270"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8M5_002270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0359505.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG8M5_002271"
     CDS             9381..9665
                     /locus_tag="ACG8M5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359506.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG8M5_002272"
     CDS             9825..10061
                     /locus_tag="ACG8M5_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0359507.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG8M5_002273"
     CDS             10058..10456
                     /locus_tag="ACG8M5_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0359508.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3721 a         1772 c         2249 g         2900 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gt
//
LOCUS       DAZKMZ010000085         9970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000085 DAZKMZ010000000
VERSION     DAZKMZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.087"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..963
                     /locus_tag="ACG8M5_002274"
     CDS             130..963
                     /locus_tag="ACG8M5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0359509.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            979..1716
                     /locus_tag="ACG8M5_002275"
     CDS             979..1716
                     /locus_tag="ACG8M5_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0359510.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1871..2839)
                     /locus_tag="ACG8M5_002276"
     CDS             complement(1871..2839)
                     /locus_tag="ACG8M5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0359511.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3071..3904
                     /locus_tag="ACG8M5_002277"
     CDS             3071..3904
                     /locus_tag="ACG8M5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0359512.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3938..4777
                     /locus_tag="ACG8M5_002278"
     CDS             3938..4777
                     /locus_tag="ACG8M5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0359513.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4803..5363
                     /locus_tag="ACG8M5_002279"
     CDS             4803..5363
                     /locus_tag="ACG8M5_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0359514.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5562..7283
                     /locus_tag="ACG8M5_002280"
     CDS             5562..7283
                     /locus_tag="ACG8M5_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0359515.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7448..8590
                     /locus_tag="ACG8M5_002281"
     CDS             7448..8590
                     /locus_tag="ACG8M5_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0359516.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8606..9817
                     /gene="thiI"
                     /locus_tag="ACG8M5_002282"
     CDS             8606..9817
                     /gene="thiI"
                     /locus_tag="ACG8M5_002282"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0359517.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3401 a         1691 c         2093 g         2785 t
ORIGIN      
        1 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
       61 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
      121 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
      181 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
      241 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
      301 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
      361 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
      421 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
      481 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
      541 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
      601 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
      661 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
      721 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
      781 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
      841 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
      901 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
      961 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     1021 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     1081 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     1141 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     1201 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     1261 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     1321 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     1381 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     1441 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     1501 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     1561 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     1621 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     1681 tattgtgaag caaatattat atggaaaaat aaataaaaaa gaggttgtga cagaaggggg
     1741 caacttcaag aaataagaag aaatttacga aaattgtttc tcaaattttt gtaaattttc
     1801 gacttagttt tcgaagaagc tatttttgtc acagcctctc tttttaagtt tagctattag
     1861 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
     1921 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
     1981 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
     2041 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
     2101 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
     2161 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
     2221 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
     2281 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
     2341 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
     2401 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
     2461 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
     2521 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
     2581 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
     2641 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
     2701 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
     2761 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
     2821 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     2881 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     2941 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     3001 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     3061 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     3121 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     3181 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     3241 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     3301 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     3361 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     3421 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     3481 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     3541 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     3601 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     3661 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     3721 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     3781 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     3841 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     3901 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     3961 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     4021 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     4081 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     4141 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     4201 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     4261 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     4321 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     4381 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     4441 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     4501 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     4561 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     4621 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     4681 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     4741 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     4801 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     4861 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     4921 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     4981 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     5041 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     5101 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     5161 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     5221 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     5281 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     5341 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     5401 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     5461 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     5521 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     5581 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     5641 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     5701 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     5761 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     5821 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     5881 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     5941 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     6001 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     6061 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     6121 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     6181 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     6241 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     6301 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     6361 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     6421 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     6481 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     6541 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     6601 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     6661 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
     6721 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
     6781 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
     6841 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
     6901 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
     6961 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
     7021 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
     7081 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
     7141 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
     7201 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
     7261 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
     7321 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
     7381 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
     7441 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
     7501 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
     7561 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
     7621 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
     7681 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
     7741 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
     7801 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
     7861 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
     7921 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
     7981 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
     8041 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
     8101 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
     8161 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
     8221 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
     8281 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
     8341 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
     8401 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
     8461 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
     8521 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
     8581 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
     8641 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
     8701 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
     8761 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
     8821 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
     8881 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
     8941 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
     9001 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
     9061 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
     9121 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
     9181 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
     9241 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
     9301 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
     9361 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
     9421 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
     9481 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
     9541 cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag aacagatcga
     9601 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
     9661 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
     9721 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
     9781 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
     9841 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
     9901 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
     9961 ttttttacta
//
LOCUS       DAZKMZ010000086         9801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000086 DAZKMZ010000000
VERSION     DAZKMZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.088"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG8M5_002283"
     CDS             complement(34..2154)
                     /locus_tag="ACG8M5_002283"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0359518.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG8M5_002284"
     CDS             2384..3562
                     /locus_tag="ACG8M5_002284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0359519.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8M5_002285"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG8M5_002285"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0359520.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8M5_002286"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG8M5_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0359521.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG8M5_002287"
     CDS             complement(4915..6168)
                     /locus_tag="ACG8M5_002287"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0359522.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8M5_002288"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG8M5_002288"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0359523.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG8M5_002289"
     CDS             7746..9092
                     /locus_tag="ACG8M5_002289"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0359524.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG8M5_002290"
     CDS             complement(9400..9786)
                     /locus_tag="ACG8M5_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0359525.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2009 g         2884 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt t
//
LOCUS       DAZKMZ010000087         9725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000087 DAZKMZ010000000
VERSION     DAZKMZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.089"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..225
                     /locus_tag="ACG8M5_002291"
     CDS             103..225
                     /locus_tag="ACG8M5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359526.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            341..1648
                     /locus_tag="ACG8M5_002292"
     CDS             341..1648
                     /locus_tag="ACG8M5_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0359527.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1767..2966
                     /locus_tag="ACG8M5_002293"
     CDS             1767..2966
                     /locus_tag="ACG8M5_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0359528.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2993..4261)
                     /locus_tag="ACG8M5_002294"
     CDS             complement(2993..4261)
                     /locus_tag="ACG8M5_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0359529.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(4316..4382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4424..5070)
                     /locus_tag="ACG8M5_002295"
                     /pseudo
     CDS             complement(4424..5070)
                     /locus_tag="ACG8M5_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(5478..5981)
                     /locus_tag="ACG8M5_002296"
     CDS             complement(5478..5981)
                     /locus_tag="ACG8M5_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0359530.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(6104..6868)
                     /locus_tag="ACG8M5_002297"
     CDS             complement(6104..6868)
                     /locus_tag="ACG8M5_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0359531.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6976..7251
                     /locus_tag="ACG8M5_002298"
     CDS             6976..7251
                     /locus_tag="ACG8M5_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0359532.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            7689..8639
                     /gene="yidC"
                     /locus_tag="ACG8M5_002299"
     CDS             7689..8639
                     /gene="yidC"
                     /locus_tag="ACG8M5_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0359533.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(8818..8997)
                     /locus_tag="ACG8M5_002300"
                     /pseudo
     CDS             complement(8818..8997)
                     /locus_tag="ACG8M5_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2935 a         2035 c         1743 g         3012 t
ORIGIN      
        1 ttaagttatc ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga
       61 aatgctgata aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt
      121 ttcagtttat taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg
      181 gaaacacagc ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc
      241 gttcttatcc taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt
      301 taatttgtag cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg
      361 tctagtccta ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc
      421 tgtgcaagct gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca
      481 aagcggtaag attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac
      541 aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg
      601 tgataaagtt tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa
      661 tgttcctttg cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat
      721 ccagtcagcc aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa
      781 attcactgaa cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt
      841 taatgcatca ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga
      901 aaacgacgaa aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt
      961 ggatttccca gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac
     1021 gcaatcacca gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa
     1081 ggaaggcgtg gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg
     1141 tacgatcact aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc
     1201 ggaaaacccg agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa
     1261 ttggtcagaa aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga
     1321 tgtaaaagac ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg
     1381 ggtacgaaac gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca
     1441 agtcactgac aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac
     1501 gatcaccttg gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga
     1561 gatcattacg gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt
     1621 cgcagaattt ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga
     1681 aagatgtttt tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata
     1741 ctgtataaga aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc
     1801 tctatcttat tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct
     1861 tgttctttta cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg
     1921 aaatttttta ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt
     1981 tttcttattt cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca
     2041 tcagtttcgg catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg
     2101 gactgatcgc cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat
     2161 tgattgtttt tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag
     2221 ctaatctttt aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt
     2281 tcttgttaaa atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct
     2341 tgatctatct atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca
     2401 accggcctcg atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag
     2461 aaacagtttc tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc
     2521 aaagccgtgc tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg
     2581 aaaaagtacc agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa
     2641 gagacattct tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg
     2701 aaatcatgga tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc
     2761 acatttttag agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg
     2821 caaaaacagc tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga
     2881 tgatgaacaa aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct
     2941 tatcgctgat tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct
     3001 tgtttttttc tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta
     3061 cttgctgagc agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca
     3121 taaagaagta tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa
     3181 tcggtgtatt tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa
     3241 cagaaacatg gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg
     3301 gtgtcacttc tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc
     3361 caataaaatc atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca
     3421 atccgagata acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta
     3481 tcgtatttcg acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga
     3541 tcgaacggct gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga
     3601 agaacagcaa catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac
     3661 catagctttc tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt
     3721 ctgttcggac tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg
     3781 tgccaaatac agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg
     3841 atttatccaa tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg
     3901 ctgttccttc acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg
     3961 taagtggaat aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta
     4021 agttgtttcc acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac
     4081 cactaactgg tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg
     4141 tcaggctatt taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga
     4201 tcaccagcca cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca
     4261 taaaaaattc ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt
     4321 agtaggcgtc attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac
     4381 ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta
     4441 cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac
     4501 tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg
     4561 tagaaagaaa aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta
     4621 tcgttggtga taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga
     4681 attttttctg cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt
     4741 cattccaagc tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt
     4801 tgctaggctg cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt
     4861 gcgcttttgt cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc
     4921 gtgatctaat atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt
     4981 tggatcattg acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa
     5041 atattggtat ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt
     5101 gcatactttg tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac
     5161 tcttttaaat aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta
     5221 tgtacatgat tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca
     5281 aacagaaaaa aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg
     5341 ttcaaaagct ggcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagcaattt
     5401 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct
     5461 attattggtt ttatctatta aatcgaggat tctttcccaa agtaacgttg ccatctctta
     5521 cgcatacttg ctgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat
     5581 gttacgatat atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc
     5641 aaaatgatat ctcgctcaag atccgacaat tcggtgatct tttctgcatt ctttacagca
     5701 attcttggat ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag
     5761 tagaacagat catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt
     5821 tttgcgagca gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga
     5881 acatgctgtg tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca
     5941 taagacatat attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct
     6001 ttttgaattt cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc
     6061 tagtcgcctt ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat
     6121 aaagaacaat ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga
     6181 tatatacgtt ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc
     6241 ccatgattat tgcgaagtta tctgattttt ctactttgtc taatgcgtgt gcttcttcat
     6301 ttaattctgt gccatacact gtaaaccctt tgtctttcat taaagaaatc acttctgtca
     6361 aatctttttg caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg
     6421 tactataaat atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg
     6481 ccgtacggat catcgtccct acatttccag gatcttgtac attatctaac aacagccatc
     6541 ctcctgaaaa atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg
     6601 gtgtaggtag atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct
     6661 ctttgtttgc ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt
     6721 cttttatttc tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat
     6781 attcttcctt ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg
     6841 gattttttgc tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa
     6901 taggtgcttg agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa
     6961 agaaaggtga cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta
     7021 ggatttcgct acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa
     7081 aacgaagatg acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa
     7141 tttattgaag aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc
     7201 caagaagacc cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa
     7261 aaagctggtc tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc
     7321 aacaggctgg acttaatgcc gtagatgcaa gcttttgaac accgtcgata aggttattct
     7381 tgaggcgctt tgcctcaaaa attaaggaca gcagtgaaaa agctgatcta tcacttgcag
     7441 tgagttcatt tgactccgct ttttcgggca gaaaaaaagt ttttagttta ttaaagtaag
     7501 aaagaaacac ttggaatcat tctacttttt cctagtattt ctttcttgat tttttgtctt
     7561 tttttcaaaa tttttcgcta cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt
     7621 gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga
     7681 agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc
     7741 tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact tcgggaatcg
     7801 tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt gaaaatttca
     7861 attggtctta tggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc
     7921 cactaggtat tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa
     7981 aaccacaagt tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa
     8041 tggcagcaca agcagaaatg cagcaagtat accgcgaaaa cggattgagt atgaccggcg
     8101 gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tactttacgg
     8161 cacgttatac agaaggtatc cgggaatcat ctttctacgg catcgattta ggctcaccaa
     8221 gtatggtact tgttgccatt gcaggggtcg cttatctgct tcaaggatac atctcaacga
     8281 tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta
     8341 tgatcgtctt catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg
     8401 gtgtgttcag ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag
     8461 cacaagtggc agaagagttg aaacaaaacc caccaaaaca agtcgtcact ccaagaattg
     8521 atgtgacaga atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa atcaagcaac
     8581 ccaaaaacag tacaaacaat aaaggccgca acgcaggaaa gcagcagaag cggaagtaga
     8641 tagtaaataa gagtgtaata aaaattcatt aacagtttta gcaattaaaa atatcgacca
     8701 agatttttga aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc
     8761 atcgattgat cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta
     8821 tctatcaacg tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ccttaatatt
     8881 ttgttttcct tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg
     8941 cgccactccc ttcgcccatg ttgctcgccc acataagaca ccattctcca taaaggacaa
     9001 cgcggagata ccacccatct aaataacatc cataaacctt tctagtagaa gaatagtgat
     9061 agtatccatc cctttttcta ctgggaatac aattacacga ttcaaagctt gatacacaac
     9121 atttcccttc ttataaaccg ctattgtaac cgaattgttt tgaatcacct ttttgggata
     9181 gaaactggct cctttccgca acttcttaaa tgttggtttc ttttatactt tctttgtagc
     9241 aactacaaat ttcttcaaat ctctaaatgt ttgcataaca accgcctttc tgacttttac
     9301 tcaggatggt atttaaaaat cagcaaaata aggcttctct ccatcttttt tatttcctag
     9361 ctgctttaaa gacactcttg ctgtttttta atctctatca agggggctct gaatctattt
     9421 tgatgaactt atcttattaa tttctcaaat gagctgtcag acacaactgt attttttcaa
     9481 tctcataccg gacaactatg ccagcttaaa aaaatttcct ctggcaaaac ttctttattg
     9541 aaaaatgaca atgaaaaaag accattcttt ttgttgaatg ctcattgtgt aaacatattt
     9601 cctgcgactg ctcttcgaac cattctcagt gcaatcccta atttatctat taaatcttct
     9661 tttagttcct catctttcat attcaaaatt tcctttgtat gttacacaag tttgttctct
     9721 aaata
//
LOCUS       DAZKMZ010000088         9593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000088 DAZKMZ010000000
VERSION     DAZKMZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.090"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8M5_002301"
     CDS             complement(<1..641)
                     /locus_tag="ACG8M5_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0359534.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8M5_002302"
     CDS             complement(643..1407)
                     /locus_tag="ACG8M5_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0359535.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8M5_002303"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8M5_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0359536.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8M5_002304"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8M5_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0359537.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8M5_002305"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8M5_002305"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8M5_002306"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8M5_002306"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0359538.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8M5_002307"
     CDS             4453..5310
                     /locus_tag="ACG8M5_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359539.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8M5_002308"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8M5_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359540.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8M5_002309"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8M5_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0359541.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8M5_002310"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8M5_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0359542.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG8M5_002311"
     CDS             8086..9567
                     /locus_tag="ACG8M5_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359543.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAZKMZ010000089         8993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000089 DAZKMZ010000000
VERSION     DAZKMZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.091"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8M5_002312"
     tRNA            2..74
                     /locus_tag="ACG8M5_002312"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8M5_002313"
     tRNA            78..150
                     /locus_tag="ACG8M5_002313"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8M5_002314"
     tRNA            155..225
                     /locus_tag="ACG8M5_002314"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8M5_002315"
     tRNA            238..311
                     /locus_tag="ACG8M5_002315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8M5_002316"
     tRNA            323..394
                     /locus_tag="ACG8M5_002316"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8M5_002317"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8M5_002317"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0359544.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8M5_002318"
     CDS             1983..3455
                     /locus_tag="ACG8M5_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0359545.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8M5_002319"
     CDS             3538..4326
                     /locus_tag="ACG8M5_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0359546.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8M5_002320"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8M5_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0359547.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8M5_002321"
     CDS             5176..6888
                     /locus_tag="ACG8M5_002321"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359548.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG8M5_002322"
     CDS             6891..8663
                     /locus_tag="ACG8M5_002322"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359549.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>8993)
                     /locus_tag="ACG8M5_002323"
     CDS             complement(8745..>8993)
                     /locus_tag="ACG8M5_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359550.1"
                     /translation="ISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLK
                     AIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2698 a         1518 c         1989 g         2788 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tat
//
LOCUS       DAZKMZ010000090         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000090 DAZKMZ010000000
VERSION     DAZKMZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.092"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8M5_002324"
     CDS             <1..260
                     /locus_tag="ACG8M5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359551.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG8M5_002325"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG8M5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG8M5_002326"
     CDS             complement(1379..1984)
                     /locus_tag="ACG8M5_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359552.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG8M5_002327"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG8M5_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG8M5_002328"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG8M5_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG8M5_002329"
     CDS             3019..3990
                     /locus_tag="ACG8M5_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359553.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG8M5_002330"
     CDS             4130..5443
                     /locus_tag="ACG8M5_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0359554.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG8M5_002331"
     CDS             5486..6352
                     /locus_tag="ACG8M5_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0359555.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG8M5_002332"
     CDS             6349..7197
                     /locus_tag="ACG8M5_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0359556.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG8M5_002333"
     CDS             7211..8683
                     /locus_tag="ACG8M5_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0359557.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG8M5_002334"
     CDS             8700..>8942
                     /locus_tag="ACG8M5_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0359558.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKMZ010000091         8677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000091 DAZKMZ010000000
VERSION     DAZKMZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.093"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..495)
                     /locus_tag="ACG8M5_002335"
     CDS             complement(49..495)
                     /locus_tag="ACG8M5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0359559.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8M5_002336"
     CDS             complement(522..698)
                     /gene="rpsU"
                     /locus_tag="ACG8M5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0359560.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(860..1288)
                     /locus_tag="ACG8M5_002337"
     CDS             complement(860..1288)
                     /locus_tag="ACG8M5_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0359561.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1380..2240
                     /locus_tag="ACG8M5_002338"
     CDS             1380..2240
                     /locus_tag="ACG8M5_002338"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0359562.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(2334..2684)
                     /locus_tag="ACG8M5_002339"
     CDS             complement(2334..2684)
                     /locus_tag="ACG8M5_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0359563.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(2700..4427)
                     /locus_tag="ACG8M5_002340"
     CDS             complement(2700..4427)
                     /locus_tag="ACG8M5_002340"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0359564.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8M5_002341"
     CDS             complement(4640..5566)
                     /gene="trxB"
                     /locus_tag="ACG8M5_002341"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0359565.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(5711..7144)
                     /locus_tag="ACG8M5_002342"
     CDS             complement(5711..7144)
                     /locus_tag="ACG8M5_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0359566.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(7144..8547)
                     /locus_tag="ACG8M5_002343"
     CDS             complement(7144..8547)
                     /locus_tag="ACG8M5_002343"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0359567.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            complement(8487..>8677)
                     /locus_tag="ACG8M5_002344"
     CDS             complement(8487..>8677)
                     /locus_tag="ACG8M5_002344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359568.1"
                     /translation="KIFTKIQKKTLTFVNVTGKKRISIVCFVYKKDNKSVKSKGVRTW
                     QKESKSLRLVEAAVTPPS"
BASE COUNT         2340 a         1946 c         1492 g         2899 t
ORIGIN      
        1 gaatttcttc accaacactc attgacgaag agcctttaaa tgataaaatt aagattgtaa
       61 tagttctttc acaatggcat ttacttggtt tccatctgct tttcctttga ctttaggcat
      121 gatcgcaccc atgactttgc caaattcttt cattgatgaa gcaccagttt gtgtgattgc
      181 ttcttgaaca agttgtcgaa tttcttcgtc ggaaagttgt tccggcatgt atttttcgac
      241 tatcacaatt tcacttttta ctttttcagc taaatcgtca cgtcctgctt cttcaaactc
      301 atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac accgttaatt cttcttcttc
      361 tgtcaagtcg tgaccttctt taatctgctc gttttgtata gatgacttga tcattcggat
      421 gacttgtaaa gattccttat ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct
      481 acttagtagt gacacactac caactccaat tcatcacaga actagaattt tttgcgtttt
      541 ctagctgctt cagatttttt cttgcgtctt acacttggtt tttcgtagaa ttcacgttta
      601 cgagattctt gtaaagtacc cgcttttgac acggaacgtt tgaagcgacg aagagcatcg
      661 tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt taattccctc cctccgagct
      721 taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa aacaagactg tcctgtaaat
      781 attatatcta atgctgtttt gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa
      841 tgttatttta tttaaatcct tattgacaat tttcacatct tccgaaaatc tcgaatctat
      901 gcccttcgat cgtacatcca ccgagctgtt cttcgaaata attcattgga cacatatgga
      961 tttctttcgt tgttccgcac accgtacaga taaagtgatg gtgatggccg atttcccctt
     1021 gacagcaatg aaaacgaaac ttcatctctc catttaaatc ggtttcttcc agtaaatcga
     1081 tttctgcaaa atcatggaga tttcgataaa tcgtatcgta acttactcct ttgaattcct
     1141 gattcatata gtcaaaaact tctttagcgg atacgtagcg attgcgcttc actaaataag
     1201 ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt atttttcaat gtttctagtg
     1261 attgttcaat caatgtctgt tttttcatga cttattcctc caaatcaaaa tgattacgaa
     1321 gtacacattc tagagtatgg tataataaac ataaaaaaaa tgcaagtgag gttttatcca
     1381 tgtcttccga ttctacagaa atcattactt tttttgtcat ctctgactct gctggagaaa
     1441 ctgcgacaaa attagcgcaa gccactatgg cccaatatcc taccgttgag ttcaatttat
     1501 tccgcagaac atttgtgaca gatgagaaaa cattgatgca ggcactgcaa gatgcactaa
     1561 aagaaaaagc ccttgtccta cacacattga tcaatcaaga attgatcgag atgacgcgtt
     1621 ctttttgtga agaacatcaa ttatttagtt ttgatgtgat gactccgccg atatcagaaa
     1681 tcgagcgctt aactggtatc aagcccattc ggcaacctgg cgcccttcat ttattgaatg
     1741 agaactattt caaacggatc aaagcaatgg aatttgctgt aaaatacgat gacgggaaag
     1801 atcctcgtgg gtttctagaa gcagatgtcg ttttgctagg cgtatccaga acttccaaaa
     1861 cgcctttgag cttgttttta gctaacaaaa atttaaaggt tgccaatcta cctttgatcc
     1921 cacaagcaca tattcccaaa cagctttggg agatcgatcc taaaaaaatc gtgggactga
     1981 cgaataaccc agatatttta aacaatatcc gtaaagaacg gatgcgctct tacggattaa
     2041 atcctgatac tgcttattcc gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg
     2101 atctttatga aaaattgggc tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa
     2161 cagcttcact tattttgaat gctttagacc tagaagatca tagttattat ggaactgaaa
     2221 ccaccgaaga aacagactaa cagtttttct tgtctttttc ttaaaaaaac tggactgtgt
     2281 cagcgaagaa gattcgttga tacagtccag tttgtctcta tttgcaccta tcgttattga
     2341 tgttccacat gtttgagcgt ctggttcaaa atatccagta cgtgagaatc atcaagacta
     2401 tacaaaatcg cttttccctc acgatgggac ttgacgacat ggttttctct taataaacgc
     2461 agttgatgcg atacagcaga ttgttccatt tctactgctt gggtgattgc tgtcacattt
     2521 tgttccccgt ctttcagata cagcagaatt ttgagacgag tggggtcact caacattttg
     2581 aatagttggc tgactttctc tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc
     2641 aacttgaaaa aagactgact tctaaagttt accacttttt ccatcagtct caagtttttt
     2701 tattcttctg tcattttttt gataatcgct tcataagcgg ctactttttc agctgcgtct
     2761 tctgaagagt ttccttttac accgatataa aatttgattt tcggctcagt tcctgaagga
     2821 cggatagcga tccagccatt ttcttccagc gtatacttca atacgtcaga tggcggtgtc
     2881 gttagttttt ctacgtttcc ttcacggtct gttctcgtca actctttgaa gtcttctgtt
     2941 tggatgactt ttgctccggc aaattctgtc ggtgcttctt cacggaattt ttccatcaaa
     3001 gcagagattt ttgcagagcc ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg
     3061 tagtagccat atgtttcaaa aatcgtttgc aagccgtcat acaatgtttt gccttgtttt
     3121 ttatagtaag ctgctacttc tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga
     3181 acgaaaggtt taactaaata accgtaactt tcttcgaatc cgaacataaa agtatgggaa
     3241 tgatcttctt caaattgttg aattttttca gcaatgaatt tgaatcctgt caatacattg
     3301 aacatggtag ccccatagtg ttccgcaatt gctgtaggca attcgctaga aacgattgat
     3361 ttcaataccg ctgcattttc aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat
     3421 tgagcaaaca gggctccgat ttggtttcct gtcaatactt cataggagcc atttggcaaa
     3481 cgaacggctg cgcctaaacg gtctgcatca ggatccgttg caatcagtaa gtcagcgcct
     3541 tctttctcgc caagtcgcat cgcatactcg aaagcagagt gttcttctgg attcggtgat
     3601 ttgactgtag tgaaatccgg gtctgcgaca gcttgttctg gaactagtac gaattgttcg
     3661 aatccagctt gtttcaacgc acgttcgccc aacatttttc ctgttccgtg aagtggtgtg
     3721 tacacaagct tcaaatcttt acccattgtt tccaccaatt catgatcaat cgttactgct
     3781 ttgatttctt ctagataagc ggcatccact tcttctccga tgatcgtgat caatccgcta
     3841 tgttcagctt cgtcgtcaga taatacttca acttttaacg gattgctgac ttcacggaca
     3901 tatttcgtca atgcatctgc gtctgcagga ggcatttgtc ccccatcttc tccgtagact
     3961 ttatatccgt tgtaagctgc tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc
     4021 ttcaaatgac ggactgcaaa agaaagctct ggtgttggac gtaaactttc gaatacataa
     4081 gaagggatat tgtgatgtgc taatgttttg gctgcttcca tggcaaattc tggtgattga
     4141 tggcgtgagt cataagcgat agcgacacct cggcgttttg tttcttcacc ttgtgtatcc
     4201 atgaaacgtg caagtccttc tgttgcttgt cttaccgtat agatgttcat tcgattgatc
     4261 ccaggtccta agattccccg catgcctgca gtaccgaatt ccaacggtgc ataaaaagca
     4321 tcttcaagtt tttcaggatc ttcacccaga tcatttaatt gtttttttaa gttttcttct
     4381 aaattttctt catgtaccca ttgttcatac acttgttccc aagacatgaa aataacacct
     4441 ctttctgaaa tatggttctt aataagtata gcattcactt attagatatg aaagtatgag
     4501 gaccaaaaaa cgcttaaatc aagaaaagat tcttttatca agcacggtta caagtattcg
     4561 cataaatcgg attcagtaga tttcacgcaa aaaaaagagt aaaagaacaa ttgaaaccgt
     4621 tctttcactc tttttaaact tatgcagttg tttcttctaa agattccaag tatttataca
     4681 cttgttgtcc agcaatccca ccatcaccaa cagcagtagt gatttgtcgc aaatcttttt
     4741 cacgaacatc tccgatggca aaaacgcctg gtatattcgt cttcatttcg ttgtctgttg
     4801 gaatccagcc ggcttcgttc gtcaacccag cttctctaaa tggtgcagtt aatggatcca
     4861 atcctacata gatgaatact ccgtctgctg ccaaatcaaa ctcattgcca gttttgacat
     4921 tacgtgcttt cacacctgtc actaccattt catttccaac gatttcgtca acgactgtat
     4981 cccacataaa ggaaattttt tcattcgcaa atgcacggtc ttggatgatc ttttgtgcac
     5041 gtaattcatc tctacggtgg acgatgacta cttcagaagc aaattgtgtc aaatagatgg
     5101 cttcttctac tgctgagtct cctcctccga tcactactag acgtttgtta cggaaaaatg
     5161 caccatcaca aactgcacag taagaaacgc cacgtccagc aaattcttcc tcgccaggta
     5221 cgcctaattt acggtggata cagcctgttg cgataatgac tgtttttcct tcgtaagttt
     5281 tatcttcaca aataacttct ttgtggctgc cgtgatcctt gatatcttgc acgattccat
     5341 aagcattttc tgtcccaaat cttgaaacac cttcatacat cttataagct agatcaggac
     5401 ccatgattga atcgaaacca ggatagtttt cgatttctgc cgtattgttc atttggccgc
     5461 caggagcgcc ttgttcgatc atcaatacgg ataagtttga acgtgaagca tataaagcag
     5521 cagtcatccc agcaggccct gcgccaataa cgattacatc atacatatgt agattctcct
     5581 ttacttaatc aagtaagcat actttacagc caatgcgaaa aaaagtctat tgttctgctt
     5641 gcgtttctcg aatagcttta ttatactcga aaaatccgct gaaaaacaat agacttttat
     5701 aaatcattga tcaatcgttc gaaagatttt ctccatttga atcaattact tttttatacc
     5761 agttgaatga tttcttcttc gaacgggcta atgtgccatt tccttcgttg tcacgatcga
     5821 cataaatgaa tccgtacctt ttcttcatct cacctgtacc agctgatact aaatcaatac
     5881 atccccaagt cgtatagcct aatagatcga ctccatctaa ttctactgca tctttcattg
     5941 cctggatatg tgctgccaga tagtcgattc gataatcatc ttccacataa ccattctcat
     6001 cgggcgtatc gactgcacct aagccgtttt ctacaataaa tagtggtttt tgataacgat
     6061 cataaatatc attcatcgtt attctcaagc ctaatggatc aatttgccaa ccccattcac
     6121 ttgcttccag atatggattt ttgacagaag caaagatatt tcctgcagtc tgttccaaga
     6181 gttcgttgtc agtagtcgaa actcgagaag aataataaga aaaggagata aaatcaacag
     6241 tatgttcttt cagcaatgcg gcatccccag gttccatttt gatctgaatg ccatttcgtt
     6301 ccatttcttt caatgcataa gcgggatatt ctcctcgtga ctggacatca atgaagaaat
     6361 aattttctcg atctgacttt ctagaagccc aaacatcttc aggattgcaa gtgtatggat
     6421 aatatgctcc tgctgccagc atacagccta ctttgttctc cggatctact tcatgagcga
     6481 tcttcgttgc gatggcactt gcaaccaact catgatgtgc cgcttgatat ttgatttgtt
     6541 cttcattttc tccttcttca aaatacagcc ctgcgcccat gaatggtgca tgaaggatca
     6601 tattgatctc attgaatgtc aaccaatatt tgacaagtcc tttatagcga ttgaagatga
     6661 ctcggcacag attttcataa aaaccaacga gttctctgga acgccacgca ccatattctt
     6721 tgatcaaatg catcggacaa tcaaaatgtg taatcgtcac aagcggctcg atcccgtact
     6781 tttgacattc tttaaagaca tcctcataaa atttcaaccc agattcattt ggctcttttt
     6841 catcgcccat tggaaaaata cgactccaag caatggacat ccgataagtc ttgaacccca
     6901 tttctgcaaa taaagcaata tcttccttat aatgatgata catatcgatc gcaatttttg
     6961 ctggataaaa atgattttca tcaaattcaa acattttctt ttttccagta atgacaggaa
     7021 aacggtcttc tccgattggc acaacatcta cgttcgctaa cccacgtccg ccttcatcat
     7081 aaccgccctc gcattgattg gctgctgtag ctccgcccca taaaaaatct tttctgaatc
     7141 ccatcatact tcctccatta catgtgtatt gcttgataat tctcttgcca gttcatcttg
     7201 gacttcgatt ccttctgctt ttaaccggct gattgtttcc ttgaattgcg gtaaatactg
     7261 ctgatgtgca ataaacaact catccatgat ccgttttgct gtctttcctg atcgtataag
     7321 cggattgatc gtaaacgctt gcaaagctgt tccataatca ccagtcacgg ctgcttcgat
     7381 cgtcagcaat tccatattct tcattacttg caaccagcct ctttcagcag caggaaggga
     7441 accaaacgct acatttcgtg cgccttgtgc gccaacatag gcagtgactt ctactacaca
     7501 atctgccggc agatcaggaa cagctccatc attctttgta gataccacga tctgtgtttc
     7561 tttatttcca tagatcgaag caatcgtttc acaggcagca tctgaataat aagcaccacc
     7621 tcgtttttgc aattgttcag gtttatgatc caattctgga tctttgtata attcaaacag
     7681 ctctgcttct gtttctttca cttgctgcgc tcttgtacca atcgtttgat attcttctaa
     7741 agcatgttcc agcatttctt cttcacgata gtaatagcgg tggtagccac aagggatcat
     7801 gttcatttgt tttaattgtt ctttgaaaaa cgggatgtca aagatatttt tcggcaatcc
     7861 attgtcttca tcgtataaac gatcaatcaa ttctgccgtg cggtcatttc ctttattatc
     7921 tgctactttg tgccaatgga agtgatttag accggcaaac ttgtagatca gctcttcttt
     7981 tttcttgcca agcattgttg gttctgtcat catcgccatt actggcacat tgcataatcc
     8041 gatcacttta tcccatttgc catatctgat aactgcttct gtcaccatcc cgcttgggtt
     8101 cgcgaaatta atcagccaag catttggaca taaacgcttc atgtcttcaa cgattgccaa
     8161 aaccactgga acggtacgaa aagctttgaa tatcccgcca gcaccatttg tttcttgccc
     8221 aatcatccca taataactag ggatccgctc gtctttgatt cgtgcatcta attgaccgac
     8281 acgaaactga gtcgtaacaa aatccgcatc tttcaatgct tcttcccgat ccagagttgc
     8341 atagattttg acttcatacg gagaagcatc ccacatacgc tgagacatct gactgacgat
     8401 ctcaagcttt tcttttcctt cttctacatc tacgagccag atttctcgta tcggtaattc
     8461 ctcatatctt ttgataaatc cttccatcaa ctcgggggtg taactgctgc ctccaccaat
     8521 cgtagcgatt ttgattcctt ttgccatgtt ctcactcctt tactttttac acttttatta
     8581 tcttttttgt aaacaaaaca aacaatagaa attcgtttct ttccagtaac atttacaaaa
     8641 gttagtgttt tcttctggat ttttgtaaat atcttgt
//
LOCUS       DAZKMZ010000092         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000092 DAZKMZ010000000
VERSION     DAZKMZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.094"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8M5_002345"
     CDS             complement(<1..149)
                     /locus_tag="ACG8M5_002345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0359569.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8M5_002346"
     CDS             complement(593..1714)
                     /locus_tag="ACG8M5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0359570.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M5_002347"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M5_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0359571.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M5_002348"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M5_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0359572.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8M5_002349"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8M5_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0359573.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8M5_002350"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8M5_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0359574.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8M5_002351"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8M5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0359575.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8M5_002352"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8M5_002352"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359576.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKMZ010000093         8337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000093 DAZKMZ010000000
VERSION     DAZKMZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.095"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..682
                     /gene="rpsN"
                     /locus_tag="ACG8M5_002353"
     CDS             413..682
                     /gene="rpsN"
                     /locus_tag="ACG8M5_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0359577.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            697..876
                     /gene="rpmF"
                     /locus_tag="ACG8M5_002354"
     CDS             697..876
                     /gene="rpmF"
                     /locus_tag="ACG8M5_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0359578.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            908..1285
                     /locus_tag="ACG8M5_002355"
     CDS             908..1285
                     /locus_tag="ACG8M5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359579.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1306..1455
                     /gene="rpmG"
                     /locus_tag="ACG8M5_002356"
     CDS             1306..1455
                     /gene="rpmG"
                     /locus_tag="ACG8M5_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0359580.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1479..2462
                     /locus_tag="ACG8M5_002357"
     CDS             1479..2462
                     /locus_tag="ACG8M5_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0359581.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2492..2614
                     /locus_tag="ACG8M5_002358"
     CDS             2492..2614
                     /locus_tag="ACG8M5_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0359582.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2632..4155
                     /locus_tag="ACG8M5_002359"
     CDS             2632..4155
                     /locus_tag="ACG8M5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0359583.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4179..4418
                     /locus_tag="ACG8M5_002360"
     CDS             4179..4418
                     /locus_tag="ACG8M5_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359584.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4611..5072)
                     /locus_tag="ACG8M5_002361"
     CDS             complement(4611..5072)
                     /locus_tag="ACG8M5_002361"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0359585.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5267..5485)
                     /locus_tag="ACG8M5_002362"
     CDS             complement(5267..5485)
                     /locus_tag="ACG8M5_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359586.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5603..7219
                     /locus_tag="ACG8M5_002363"
     CDS             5603..7219
                     /locus_tag="ACG8M5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0359587.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7460..>8337)
                     /locus_tag="ACG8M5_002364"
     CDS             complement(7460..>8337)
                     /locus_tag="ACG8M5_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0359588.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2858 a         1281 c         1662 g         2536 t
ORIGIN      
        1 tatgattgaa ttagttcact taatgtctat tcttataata taaggaataa tcaaagtttt
       61 aaaaaattgg cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt
      121 catattgccg ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac
      181 taataaaagt gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga
      241 ttatctctat gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt
      301 tcatgttatt tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt
      361 aacgattacg atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa
      421 aaagtcgaaa attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa
      481 acgtcaggaa ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc
      541 aaatccgaat cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg
      601 aaagttcggt atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg
      661 agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca
      721 tctaaggcga agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca
      781 tttgacgaag cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat
      841 aatggaaaga aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg
      901 aatcaaaatg gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga
      961 aacgacccag caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat
     1021 tattactgaa acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta
     1081 ctatgaggac caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga
     1141 gtatgacgag gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa
     1201 agaaaattta gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat
     1261 tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt
     1321 attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat
     1381 aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt
     1441 aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg
     1501 tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac
     1561 cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc
     1621 acaaattatt agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt
     1681 gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac
     1741 atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa
     1801 tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat
     1861 tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc
     1921 aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac
     1981 taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca
     2041 cacatagtga aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct
     2101 tagtttttga aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc
     2161 atcaagtaca tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt
     2221 ttttactcca atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta
     2281 aaggattgat tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac
     2341 aggttaattt tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa
     2401 aaatgttgga tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat
     2461 aatgataagt agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata
     2521 tcgacgactt aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga
     2581 acataaacga aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa
     2641 atcagtgtag gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact
     2701 aatgatgcaa aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat
     2761 ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt
     2821 ccagcgggat cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat
     2881 gatgctgatg tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa
     2941 gaaagttgga agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg
     3001 ccaggtagtg aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat
     3061 cctcatacgt gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag
     3121 ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg
     3181 actaaactga aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa
     3241 agttttgtga cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa
     3301 gttcctattg ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta
     3361 aaagagtatg ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat
     3421 aaaattgcaa aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt
     3481 gaaagtctta caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac
     3541 aatctaaaag cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa
     3601 gaagcgaagg atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat
     3661 cgcgaattga gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga
     3721 attttggatg aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag
     3781 tacaaagatt attatactac tggctataag acagatattg atactatcaa cattaaagac
     3841 aacaccattg attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga
     3901 tacaaaattt tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat
     3961 gatgcaggtg ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt
     4021 aaggcagagc atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa
     4081 ttgcataatt ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa
     4141 atgatggctc attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt
     4201 ctatggattc caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat
     4261 taaatgggag tacattagct gatattgctg caactttaca tgttagtatt gacacagttc
     4321 atagagtatt aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta
     4381 gacgtttagc aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca
     4441 tttttccctt atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg
     4501 caacacttta tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct
     4561 gttattcagt ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt
     4621 cgattttggc aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca
     4681 agggcatgag gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa
     4741 tgattggaga attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata
     4801 ctgcttaaaa gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc
     4861 tttcctgcta aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca
     4921 gttttgccag ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat
     4981 tttggacggc catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt
     5041 tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt
     5101 gagaggggtt ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta
     5161 attgaaaagt ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga
     5221 aaatcgacaa ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa
     5281 cgtccgcatt tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt
     5341 ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc
     5401 caattatact aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta
     5461 tagtaagatg acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact
     5521 tatactagtt ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc
     5581 aaggtataga aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct
     5641 tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat
     5701 tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa
     5761 aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac
     5821 attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca
     5881 aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt
     5941 agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat
     6001 agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag
     6061 aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca
     6121 attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat
     6181 ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat
     6241 taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac
     6301 ttcctattca gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga
     6361 aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca
     6421 aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt
     6481 tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa
     6541 aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat
     6601 tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa
     6661 taaactagtt tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg
     6721 ttgtttaact gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa
     6781 aaagtctaat atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa
     6841 gataggcgat gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt
     6901 attctctata gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg
     6961 aattgatggc aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa
     7021 ttatcatcct gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt
     7081 taaagaagat aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg
     7141 tcaaacgagt atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac
     7201 agattactac atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat
     7261 tattattaat aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag
     7321 aagcggtgac aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc
     7381 ggttagatta cttattttgg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     7441 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     7501 tagtcagttg ggtagtattc ttagttttct taaaatattt tcctcgatat aaaaagattc
     7561 gttctcggta gttttgaaag tttctatagc cataggcaat tcgttttagt actttgatat
     7621 ggttattcaa acattctata gggccgttgg aatagggaac gcgcaacgct cgtttgattt
     7681 ctgtttggta tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt
     7741 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata acggaaagaa
     7801 aatcctggta gacttcgtag cctcgcttta aggaatcatc ataagataat agccgatcga
     7861 ctatctctgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgacctt
     7921 tcaattccca cgcattcttt tgaagtagtt tccagtattt ctttaagtgc ttcgaccgac
     7981 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaaa gcccgaccaa
     8041 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     8101 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgcacttgt tcacggactt
     8161 ttatagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     8221 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacatttt
     8281 tcactgattt gaattcatcg aaacacacca cttctggtaa cagctggctc aaagaaa
//
LOCUS       DAZKMZ010000094         8275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000094 DAZKMZ010000000
VERSION     DAZKMZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.096"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8M5_002365"
     CDS             476..1339
                     /locus_tag="ACG8M5_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0359589.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8M5_002366"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8M5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0359590.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8M5_002367"
     CDS             3339..3860
                     /locus_tag="ACG8M5_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0359591.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8M5_002368"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8M5_002368"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0359592.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8M5_002369"
     CDS             5941..6759
                     /locus_tag="ACG8M5_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0359593.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8M5_002370"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8M5_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359594.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8M5_002371"
     CDS             7228..7806
                     /locus_tag="ACG8M5_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0359595.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8M5_002372"
     CDS             7901..8038
                     /locus_tag="ACG8M5_002372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359596.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2491 a         1485 c         1632 g         2667 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaa
//
LOCUS       DAZKMZ010000095         8148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000095 DAZKMZ010000000
VERSION     DAZKMZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.097"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..307
                     /locus_tag="ACG8M5_002373"
     CDS             83..307
                     /locus_tag="ACG8M5_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0359597.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            449..2200
                     /locus_tag="ACG8M5_002374"
     CDS             449..2200
                     /locus_tag="ACG8M5_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0359598.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            2200..3984
                     /locus_tag="ACG8M5_002375"
     CDS             2200..3984
                     /locus_tag="ACG8M5_002375"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359599.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            4319..5171
                     /gene="licT"
                     /locus_tag="ACG8M5_002376"
                     /pseudo
     CDS             4319..5171
                     /gene="licT"
                     /locus_tag="ACG8M5_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            5268..7154
                     /locus_tag="ACG8M5_002377"
     CDS             5268..7154
                     /locus_tag="ACG8M5_002377"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0359600.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(7195..8097)
                     /locus_tag="ACG8M5_002378"
     CDS             complement(7195..8097)
                     /locus_tag="ACG8M5_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0359601.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
BASE COUNT         2536 a         1417 c         1777 g         2418 t
ORIGIN      
        1 aagaatacgc ctttcttttt tcaataaact ttagtaaaat aaatcattga gagcaattcg
       61 gaaatagaaa ggagagattt atatggtatc aacaggtatt gtcgtactta ttgctattat
      121 tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata tgaaagacta
      181 tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga tgtcaatggg
      241 gcaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga accaagggaa
      301 aaaataggtt ctgacccaaa gactttttgc gggctgattt tttcagtccg ttttttattt
      361 cgtaatttgt tagactattc agactattca gcaaaaaaga caagcacaga caatcctatg
      421 ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaaattag gttggttttt
      481 tcgtcaggaa aagaaacatt atttaattgg gattttctcc ctattcgcag tggccttggt
      541 acagttagtt cctccaaaag tgattgggat catcattgat gagatcgccg ataagaattt
      601 atcaataaag atcatttcct ggtggatctt attattgatc cttgcagctt tcttacaata
      661 tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcggt tggagagaga
      721 acttcggcag cagctattca aacattttac agaaatggac aatctctttt accaaaaata
      781 tcggactgga gatttgatgg ctcatgcaac gaatgatttg aatgcaatcc agaatgtagc
      841 aggagctgga atcttgacct ttgcagattc agtgattacg ggcggaacaa cgatcattgc
      901 gatgattttg tttattgatt ggcgcttaac actgattgcg ctgattcctc tgcctttgct
      961 agccgttact tcacgagtgc tggggtcaaa actccacgat gcttttcgtg attcgcaaga
     1021 agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca aagtcattaa
     1081 aacatttgga caagaaaaag aagatctcgc ggattttgaa gaaaagattg aagatgcaat
     1141 tattaaaaac aaacgagtca attttttaga ctccttattt gatccattca ttaccttgat
     1201 tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct tggaaggcac
     1261 gatctcaatc gggcagatgg tctcatttat tagctatatc ggtatgcttg tttggccgat
     1321 gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt atgatcgcgt
     1381 caatgaactt ttgcatgaaa agacgcatat cgtggaacaa ctgggtgcag tgcagacccc
     1441 ggcaaaaggc gagctgaatg tagctatttc ctcttttaac tatccgaaag aagaatcacc
     1501 agcattagaa caaatcaaat ttgcgatcca agaaggagaa acattaggta ttgttgggaa
     1561 aactggtgcg ggaaaaacga caatcatcaa attactgatg cgtgaatacg atgattatca
     1621 agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg ctttattagg
     1681 atcaatcggt tatgtgcctc aggatcactt cctgttttcg atgaccattc gagataatat
     1741 ccgtttttct aatccaaaac tttcagaaga agaagtagag catgcggctt ctttagcatt
     1801 tatcaacaat gaaatcaagg aattgccaaa aggttatgat accttagttg gagaacgtgg
     1861 agtctcttta tctggcggac agaaacagag gatttcgatt gctcgagcat tgatcaaaaa
     1921 tcctgaattg ctgatcttag atgatgcact ttctgcagta gatgccaaaa cagaagaagc
     1981 tatcttgcat aatttaaaag aaataagaaa agagaaaaca accatcattg cagctcaccg
     2041 tttaagcagc gtgatgcatg caaaagaaat cattgttgtc gatcaaggga aaatcattga
     2101 acgaggaacc caccacgagc tgcttgcatt aggtggctgg tatgaacgaa tgtgggaaaa
     2161 acagcaatta gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa ttatcaatcg
     2221 gaatggacaa aatcgattcc acttaaagaa cagaagaaga tcatcaaacg actgatgaag
     2281 ttcgccaaac cttttagaag aacgttcatc gttgctatct tatttgcgtt tgccctgtct
     2341 gtgatcaatg tcctattgcc aaggatcatc caaactttta tggatgatca tctggctaaa
     2401 cagtcagcta cgacacaagt tattttgttt tttgctggtg tgtacttgtt tggagtcata
     2461 gtcaaaagta tcatttggtt tttccaatgg tatttgtatt ccatggcttc attgaagacc
     2521 tatcagtatg ttcgggtaag attatttgaa aagctgcata cactaggtat gcgttatttt
     2581 gaccagacac cggcaggatc gaccgtttct cgagtgacga atgacacaga aacgctattt
     2641 gaattttggt atgtcttttt gatggtattg actgggatct ttgcagtcgt ttcttctttc
     2701 gttgccatgt ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt cttgccgatt
     2761 ttaggaatcg tcatctggta ttaccagaaa ttcagttctc gtatttaccg aaatatgcgg
     2821 gaaaaattaa gtcagctgaa cacgaaattg aatgaatcga tttccggcat gcgtattatc
     2881 cagcagtttc gccaagaagc tcgtttagct aaagaatttg aacaagtgaa caacgagtat
     2941 ttagacacgc gctatgcgat gatcaaaacg aattcgattc tgttaagtcc aatcatcaat
     3001 tttctctatg cattggcgat tgcgttggcg ttgactttgt ttgggatcga tgcgttgcat
     3061 tctcccgtag aagttggact gatctatgca tttacgacct atgtccagga attttttaat
     3121 ccaatgtctc aaatgatgga ctttttgagc acctttacag atgggatggt tgccggtagt
     3181 cgtattttga aaatcatgga tacagaagaa tatgcaccgc aacaaaatcc tggagcagat
     3241 caagcgatta cttcaggtaa gatcgagttt cgcaatgtaa gcttttctta tgatggagag
     3301 cacaatgtat tgaacaatat ctcttttgtc gctaatccgg gtgagaccgt tgcattagtg
     3361 gggcacaccg gaagcggaaa aagctcgatc atcaacgtat tgatgcggtt ttacgaattt
     3421 tatgaaggcc aaatcttgat tgatgatcat gacattcgag aatatccaat ggaagagtta
     3481 cgtaagaaaa tgggattggt cttgcaagat gccttcttgt tttatggaga tattgcagga
     3541 aatatccgga tgatgaatcc agctatcaca gacgaacaga ttcgccatgc agctgagttt
     3601 gttcaagcaa acaagttcat cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa
     3661 cacggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg aacaatcgtg
     3721 acagatccta aaatactagt attggatgaa gcaacagcaa atatcgatac tgaaaccgaa
     3781 ggattgatcc aagaaggatt ggcaaaaatg cgacaaggac ggacaacgat agcgattgcc
     3841 catcggctgt ccacgattcg ggatgcagaa ttgattttag ttttagacaa aggtcatata
     3901 gtagagcgtg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc agatatgtac
     3961 aaactgcaaa gcagtggtgt gtaaaaagag gttgtgacag tagcatagta gcattttgcc
     4021 ctaagcgcta gagtgaaaat aaaacagagc ttcgcagatt cttcatctgt gagcgttagt
     4081 ggcgtagtca cccgatttaa aaaagacgac gacagctagt caaaagcggc taataaccac
     4141 taagaaagcg ttgacaaaag cgtttgcttt cgataatata aagaaaacga tacaacttgg
     4201 atagtgattg cctgatgcaa gctaaacctg atgagtctcc catggttcca tggacggctt
     4261 tttgggtttt tctttttgtt ttggatacag ccctatatat catggaaagg agcaaaacat
     4321 gctgattgag aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga
     4381 aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga
     4441 ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga
     4501 attggcacaa gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac
     4561 aaaagaggct ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt
     4621 gtatacggct atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga
     4681 tatcaaacaa ctatttccta aagagtatgc catcgggaaa aaaaacagtg aagaaaatct
     4741 cggataaata ccacatcaag ttatcagaag atgaagccgg atttatcgca ttgcatttag
     4801 tcaatgcaca aggcattcaa agccaacaag acaataccta cctgttcact ataacaatac
     4861 aggatattct aaatatcgtc cgctattact ataagattac atttgatgaa gattcagtcc
     4921 atttttttcg attcgtcaca cacttacgtt tttttctgac acgcgtccta gatggagaaa
     4981 aatcagaagt gggagaagtt tctgaggaat tgtttcaggt tattcaaaaa aaatataagg
     5041 atacgtttgg ttgcgttgag aaaatagcag actttttagc cgagacgttc aactaccaga
     5101 tgtccacgga cgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc
     5161 gttgtaaata aataaaaaaa ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg
     5221 acattcagtt ggctaaacct tccactaata aattggaggg aatagaaatg ggaaaatacc
     5281 aagaattagc gagaaaaatc gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga
     5341 cacattgtat cacaaggctt cgtttcaaat taaaaaacga agaaaaagca aatgatgaga
     5401 tcttgaaaaa tatggacggt attgtaacgg taatgcgagc gggagggcag tatcaagttg
     5461 tcatcgggaa tcatgtgcca gtcgtgtttg aagaggttat aaaagcagga aaccttactt
     5521 ttgatgaggc tgtttcaaca gaaaaaatga gaccgtttga tatgcttatt gatggaatat
     5581 cgggctgttt ccaaccattc cttgctatcc tagctgctga cgggatgatt cgtggactga
     5641 cagcattcct tgtcttctta ggcgcgtttg accgcggttc tggcacattt gttatgtttg
     5701 ataatatcgg tgattctgta ttccaattta tgcctgttat tatcggttta acagctgccc
     5761 gtaaatttaa agtaaatgaa ttcgtaggga tgttgcttgg cgcagcatta atgaatccta
     5821 gcttgtcgct ggaagctttg agtggtgcag cagaagcacc actaacgacc atattttctg
     5881 gcactatttt tgaagcacct atttatcaaa cagtattcgg cattccctgg attgcgcgta
     5941 actacgcttc ttctgtcatt cccattatct ttattgtgtt gttagctagc caaattcaga
     6001 aaccaattaa aaagttagta cctgagatga tcgcgaattt cttcgttcca ttcttcaccg
     6061 ttcttattac aatgccttta ggttttttac tcgttggacc agtattcaca ttcgcaactg
     6121 atattttaat ggctggattt gaaacacttt tagcactttc gccagttatt tatggagcaa
     6181 tcgtaggctt cttctggcaa attctggtga tgtttggtct ccattgggca atcgtaccaa
     6241 tgggattgat gcaattttct gtgaatggtt ggcaaaatat tatgacacct gttgcagtag
     6301 tgtcttttgg acaaactgca gcacttaccg cactctactt taagcttaga aatccaaaag
     6361 acaaagcaat tgcaatacca gccattgttt ctggtattgt ggggattaca gagccagcta
     6421 tttatgggtt tacattgcca cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt
     6481 tagggggcgc gtatagtggg ttaatgaatt taacttcttg gaatcaaggt ggactaggca
     6541 tctttacaat tcccaactat attagaccag atggtgattt aacagatgtg attaatgtat
     6601 tgattgggat cgccattgcg atggtggtat cctttgcgct gacttttttc ttttggaagg
     6661 aggaagcaga tgaaacagat gatagacaga aaagatcagg taaagaaatt gtaaaaacac
     6721 cgatacaagg acaaatcgct ccactgaatg cagcaaagga tgcagctttt gcacaaggaa
     6781 cactaggtcg aggaatcttg atctaccctg aaaaaggaga agtacgtgcc ccttttgacg
     6841 gaacagtcat gtccctgttt ccgacgaaac atgcaatcgg gatggtttct gaagcaggtc
     6901 tggaattgct gattcatgtc ggattggata ctgttcagtt ggaagggaaa tattttgaaa
     6961 gcttggtgca gcaaggagaa agagtgaaac aaggcgatct gttgcttcgc ttcgacaaaa
     7021 agaaaatcga agaggaagga tacagtttag aaacacctgt gattgtcatc aattacatgg
     7081 attatttgga tattttggaa aatcaagggg aaacggtcgg gccagacgat gcacttatca
     7141 ccgcactgac ctgaacggtt aggtgaaaga atggactagg cttaactatt ctttttagtc
     7201 cacttcttta ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt
     7261 atcaaatttt tgtcctacag aaattagatg gactaaattc tgttgataat tttctgcaaa
     7321 aacacaacta tataacacgt ctagaagata acaaatcgat gtattgatcg taaaattacc
     7381 aattttcgaa tacagtcgtt cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgaaga
     7441 tagcgttgaa tcaccgatac tggtcaaagc taaaattggc acatgatttt gtttcaatat
     7501 ttctgcggta cgaaggatca cattgttttc acccgtataa gaaatcagaa tcccacacat
     7561 atcacttgtg caattgtacg cttcatagaa cgattcacca aaagtggtga caatcgtgac
     7621 atccattttg atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt
     7681 actggcgaaa atcacgattt tttttgcttg aagcattagt tgtttggctt tttgcagttg
     7741 gtcatgatga atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt
     7801 atttgcgatc gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg
     7861 aaaattccct tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc
     7921 atcaaaaccc attttttttg ctacacggat gagtgtggac gggtggacat aacatcgctc
     7981 agcaatctct tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactgaat
     8041 cacttcattt tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta
     8101 gataccttct tcttgttttt ttacgtaaat cataactttt ttgaaaat
//
LOCUS       DAZKMZ010000096         7990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000096 DAZKMZ010000000
VERSION     DAZKMZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.099"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..454)
                     /locus_tag="ACG8M5_002379"
     CDS             complement(<1..454)
                     /locus_tag="ACG8M5_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359602.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKL"
     gene            613..930
                     /locus_tag="ACG8M5_002380"
     CDS             613..930
                     /locus_tag="ACG8M5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359603.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            863..1342
                     /locus_tag="ACG8M5_002381"
     CDS             863..1342
                     /locus_tag="ACG8M5_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0359604.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1359..1535
                     /locus_tag="ACG8M5_002382"
     CDS             1359..1535
                     /locus_tag="ACG8M5_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359605.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1792..2223
                     /locus_tag="ACG8M5_002383"
     CDS             1792..2223
                     /locus_tag="ACG8M5_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359606.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2229..2546
                     /locus_tag="ACG8M5_002384"
     CDS             2229..2546
                     /locus_tag="ACG8M5_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0359607.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2665..3351
                     /locus_tag="ACG8M5_002385"
     CDS             2665..3351
                     /locus_tag="ACG8M5_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0359608.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3326..4675
                     /locus_tag="ACG8M5_002386"
     CDS             3326..4675
                     /locus_tag="ACG8M5_002386"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0359609.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4666..5139
                     /locus_tag="ACG8M5_002387"
     CDS             4666..5139
                     /locus_tag="ACG8M5_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0359610.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5141..5641
                     /locus_tag="ACG8M5_002388"
     CDS             5141..5641
                     /locus_tag="ACG8M5_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0359611.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5656..7968
                     /locus_tag="ACG8M5_002389"
     CDS             5656..7968
                     /locus_tag="ACG8M5_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0359612.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         2828 a         1349 c         1769 g         2044 t
ORIGIN      
        1 ttagctttaa cttaaatttt ttttgataat tagctgttct tatgaccatt acaagttttt
       61 caccaggtaa tatatctttc atatctaaaa cttctgaaaa agcgactgag tagccaggag
      121 agattgcttg tgccaacggt ataggagata aaaggctact tttatgaatg cttcccatat
      181 caaaaccatt ggcgtgataa tgtgaacaat taattttctt accgttgtaa agatacgtac
      241 attcgataat tgagtttggc atttttgatg ggtttgcaat tacgccagta agaatcattc
      301 gattacttct atcatgctta actaaaaaat aggagagcgt aatttttctt ttatctttaa
      361 ataagttata gagggataaa aaaaatcctg aaatagaaat gattaaagta atagcgactt
      421 ggtaatcgct caaaattttt atcaagtaat tcaccacctt tttactcaaa ttatatcaaa
      481 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaaaaaa gacacgaacg
      541 ccggcaagca tgatcgtgtc agggaaataa actatctttc tatattttac cacagaaagg
      601 aatgaaccgt aaatgctaat tgcaacggat acactagacc gcatcttttt aaaagacgaa
      661 tacaaactgc gcaatataga tgcgtcagga attttagtat ttgatcttta tgacaatgga
      721 aaaattggta tctatcaagc aagtgatatt gaagaaacaa accttgcttt cgaacaaatt
      781 gatgattctg tggaattgga tttagatgag gcaatcctag cttttgaaca aattgcaaaa
      841 ttattaaagg aggcacaaaa gaatggcaac tctttaccaa ctcagcgagt catatatcaa
      901 agtcctggaa ctggcagaac aattggatga agaaattctt cgcgatactc tcgattcgat
      961 taatgaagcg atcgagtata aggcagaaaa cttagcaaaa atagttaaag aagtagaagg
     1021 gaaagctgag ttaatcgata gtgaaattaa acgtctgcag gaacgtaaga catcgctttt
     1081 gaacaatgct aagagtatca agcactattt acaagaggaa atggaaaaga ctggtaaaac
     1141 gaagattaaa ggtgaattat tcaacattgg aattcaaaat aatccagtat cggtcaatgt
     1201 aatcaatgag aacttaattc caaaaggatt ctttacccct gtgcctccca aattggataa
     1261 aaagcaattg aaggaggaac tgaagcacgg agatattcct ggtgctgaac tcgtccaaac
     1321 aaaaggtttg aggataagat gatgaaggag gagaaataat ggaacacgaa aacgtaaaag
     1381 acgctttgaa agctgctatc gaaatcgcgg aagctaaagg aataaaggtt gacggcaaac
     1441 cagcaactgt ccatgacatt caaaatttga cgaaagaaca tctttatttt atcgccgact
     1501 tgcttggttt gtcggaattg tatttagaca aataacccca cacggaagga gtggctaggg
     1561 tggttgtatt acaagtaata gtctgcgtaa cattcatttg gtcgtgcata ccggtttgta
     1621 tatatatcat gaagaagctt cgccctgagt cttacaaatt atacgcaatt tacagcataa
     1681 cagtagctct tgcatttaca ttgtttgcaa tttctaggct tccgttttaa tcgtgggaat
     1741 gaatttttaa tgaactaaat tatctataaa gcaaaaaaag gagaaacaac attgaaaaac
     1801 aaactagcaa aaacaacagc aatcatcgga ctagcactag gtagcggcgt tatcggctac
     1861 gcagcaagca atgcgtttca agatttggac acgatcaaag caaatttcaa cacagtccta
     1921 caatacggtc aaacgaaatc gcaacgtgtg tcagaacttg aatcacagct atccaacaac
     1981 actcgcacac aggagcagct gaaagccgaa attgagcaaa tcaaatcgga caagcagaag
     2041 gaaattgaag ccaagcaacg agaagtcgaa caaaagcaac aggagatcgc tacaaagcaa
     2101 caggaagccg atagcttgcg ccaacaacta tccacagtgc aaaacgacaa ggagcagtta
     2161 gaacagcgcg tgagcgagtt acggcagtat acggatcaaa aggtagggga gttggggaaa
     2221 tgaaagagtt gactatttat gacccaaata tttcatgggc taaacacaca attaaagttt
     2281 cttttatgat ttggggttac aaaggatatg ttacttacaa agtgggcggt aacactaagg
     2341 gtttatcatt aatagctatt gattcagatg atctatatga tgctaatttc gaggataacc
     2401 cagttaattt ccgtgacctt gatgaagatt ggttctctat ggaacttact aacgataaag
     2461 gcgacagtac acttgtagaa gatgaattcg acagactagg tgattatatt gtcggtgttg
     2521 aaattattgc gcacgaacca gagtaggaga gtgaatacaa aatgattaaa agaatcacta
     2581 tatcagttga agaagatagt ggatcaacaa tgcagcatac cgacaatcga aaaacttttt
     2641 taaccaatag aaaggagaat tttgatgcag attaagaagg cgacggatat cgaacaaacc
     2701 aagggaacat atttgatcta cggtgctcca ggaaaaggta aaacttcaac ggctaaatat
     2761 tttccaggga aaacactgat ccttgatatt gatcggactt caaaagtcct tagaggtgag
     2821 aaaaatgtcg atatcgtata tatcgacaac gaagatacct ggaacgactg gggcaacacg
     2881 ttggctgatt taactaccaa ttatgtaggt gtttatgaca acgtggttgt cgataatgtc
     2941 agcgaattgg aacggtgtat tctgtccagt ttaggcgcag aaggaaaaaa caacggcgtt
     3001 ccttcgcaag gcgattatca atatatgcaa tttagaatgg tgaacagcct acgctatatg
     3061 aaaaatttag acagcaacct tatctggacc gcatgggaag aaatcgattt gtggacggat
     3121 tcggatggat cttcttacaa catcgctttg ccgcagatca atcgaaagat tcgaaacaat
     3181 attttgggat tatgtgacgt tgttggtcga ttgatggtaa aggaagacga tgaacgcgga
     3241 tttattttac aagccacaga ttcaacctat gccaaaaatc aattggataa tagatctggc
     3301 tgtaagcaaa gtgagctgat tttagatgga attgtacgac taccagaatg atttagtcga
     3361 tcgtgcaaga caagcctatg ttgatggtta caaggcacca tgtatcgtat caccgtgcgg
     3421 ttctggaaaa tcagtcatgg ttgcagagat cgctaaacga accacacgaa aaggcaacag
     3481 agtgttgttc ttggtccatc gtcgtgaatt gattgatcag atcaaagata cgtttcaaaa
     3541 gatgggcgta gataatcagc ttgtaaattt cggcatggtt caaacagtcg tacgacatct
     3601 taaaaccatc aagaaaccac aactgatcat cactgacgaa aaccatcacg ggttagcagc
     3661 gtcctacaga aaaatctatg agtattattc ggacgttccg cgattaggtt ttaccgcgac
     3721 acccatccgg ttaaacggta gtgggttggg ggatgtgaac gacttgctaa ttgaaggagt
     3781 atcggctaaa tggttgattg agaatcatag attagctcct tatgaatatt atgcacctaa
     3841 attgatcgat acagccgaat taaagaaagc ttcgactgga gattttacaa agaaatcaat
     3901 ggataaggca gtgaagaata ctatttatgg cgacgtgttg aaacattata aaacgttagc
     3961 agaaggtgaa caggcaatag catactgtca cagtatcgat gcaagtaaac acactgctga
     4021 aatattcaac gatgctggtt ataaagcaac acatatcgat gcaaaaactc caaaagatga
     4081 acgtgcagat attatcgaag cgttcagaaa tcatgaaatt aaaatcctat gcaatgttga
     4141 tttgatcgga gaagggtttg atgttcctga ttgttcgact gtaattatgt tgagacctac
     4201 tcaatcgtta tcattgtaca ttcagcaatc gatgaggggg atgcgttacc gaccagataa
     4261 aacgtcaatc atcattgacc atgtgggtaa tgtgaaccgt tttggattgc ctgatatgga
     4321 tcgtgaatgg agtttagata caaaaaagaa aacaaaaact gatagtgatt tatctatcgt
     4381 tcaatgcgcg ttttgttttg gtgcttacga acggccaaaa ggcgataata tttgtccgta
     4441 ttgcggagag ttgcagccga ttgaagaacg aaagtcagag ttagaaatcg atgaatcggc
     4501 tgaattaatg aaagtcggag aaacgaaaat tactttgaat tttgagaaca ataaatatta
     4561 caacatgacg gaagatgaag cagaaagtat agaggactta tatgccatag caagagctaa
     4621 aggatttaaa cctggttggg cttatatggc tgctaagagg aaggggtggt tgtaggaatg
     4681 ggcggtattt ttggtaaaaa agcagaagat gttcaaggtt caataataaa tggcgtaaca
     4741 ataataggtg atactggaaa aagagatagt tcaggcaatc aaattgtaat cgctagaaat
     4801 aaaaataacg aagtttttga atcttctcta tctaatataa aaagaggtgt tgttaacggg
     4861 acaggcgaag agagtggcta cgttaattta aaaaaagcta atgaaaaata ccatgttgaa
     4921 ggaacttacg ttaaggcttt gtctgtcggt ggaacaaaag gcgttaattg ggataaacga
     4981 agaaataaat ggaaagcacg attatatttc aaaggagaat atgtcttaca aaaggatttc
     5041 aacaccaaag aagaagcaat ccaagcacga cttgcagcag aagaaaaata tttcaaacct
     5101 atcttagaaa aatatgaaaa taaaaaggag aatgattaac atgacaggat ttaacttaga
     5161 cttttcaaaa gcgcaacaag ggaacgaaat caaagacggt acgtacgaag tggtcgtaaa
     5221 caaagctgta gaaaatgcaa ctaaatcagg cgcagaattt atcgatatcg atttgatcgt
     5281 tcgtaacgat gtggatcaac cgttccaaaa taaacacatt ttcgcaaaaa tctggaaagc
     5341 caaagctacc ggcaaatata acgaaggtat gatcatggca attgcacaag cgctacagtt
     5401 ggaagacgga aaatcatata acgggtttga tgaattgctg gcggactttg ttttgaaaac
     5461 cgcctcagta cgcgtgaaaa ctgaagagtc aaatggatat aaaaacgtca atgtgaagtc
     5521 ttgggataaa acgaacacac ggggcgtaat gaatcaccaa ttcaaaaatg gtgatgaacc
     5581 atccttcgga ccggaacgtt caagagcgac aacggttaga aatgacaatc ttccatttta
     5641 attgaggtgt aatcaatgta tgaaaaaatt ccaaaagagc ttaaaaatct caaacaatgg
     5701 tgtgtttata agctagtttg ggatgaaaag cggcagaagc acacgaagat cccttataac
     5761 gccaacaacg ggcataaagc caaatccaat gacgagagta cctggtctga ttttcagacc
     5821 gctctcgctg ctattgatca ctacggaatg agtggactgg ggttcttttt caaaccacct
     5881 tatttcggca ttgatatcga taatgctgaa ggtgaagttg agcgatttaa atcaggtgat
     5941 atagaagaaa atattatcta tgaatttatc gaaagcatga gatcctatgc tgagtattct
     6001 caatcaggta ctggcatcca cattattgct cgcggagaat tgcccggcgg aagacgcagg
     6061 aaaggtgacg tagaaatgta ccagaacggc cgattcttcg ttatgactgg aaatgccgca
     6121 tcaaaatatt tagaaataac tgagccgaat ccaaaggata tcaaacgcct atatgatcgc
     6181 tacgttggag atgaaaaaat catccaattc aaagaagaaa atccgctgat gaataccgtt
     6241 gatttaccgg ttgaagaaat tatcaaacgt gctgaatcgt cttcccaagg ggcaagattc
     6301 aaagtcttta tgaacggcgg ttgggaagct ttttattcct cgcaatccga agcggatatg
     6361 gcgttcgcca acgacttggc tttttggacc ggtagagact ttgaaaaaat ggatgagatt
     6421 ttcagagcgt ctgcaatgat ccgtagcaaa tatgaccaaa aaaggggatc ggcaacttac
     6481 ggagaatctc tactaaataa ggcaattgct gatacaaatt ccgtctacaa ccccaaacgg
     6541 aaatctgatt ttaagatttt tatcaaagac caggaacagc caaaagagga aaaatactat
     6601 tcgtacgacg acaccggcaa cgcggatcgg tttactgata tctacggaac gttagtcaaa
     6661 tattcgtaca ttgataaatc ttggtattac tacgatggga aagtttggct acaggacaat
     6721 accggcgaag ttcggaaaat gatcgatacg acagtagaca tcatgggaaa agaaccgtta
     6781 acgatccctg aaggcgcaga cgatgaaacc aaagaagctt tgatgaaagc gaaagaaaaa
     6841 cacgtcaagc gttctcgcag caatgccgga aaaaacgcta tgatggacga attgaaacat
     6901 cgactatccg tattgccgga agagttcgac aaagataaaa cgctgttcaa cacacaaagc
     6961 ggctatctgt ctttgcatga cggtcttcta cacgagcatg agatagacaa aatgtttaca
     7021 cgtgtttcaa atgtcgagta cacagaatcg gtcgattgtc ctatgtggga agaatttatt
     7081 aatcagattt ttgacaatga tcaagaatta atcagatata tccagaaatg tgtcggatat
     7141 tccctgaccg gatctaccag agagcaatgt atgttcatcc tctacggtca tgggtcaaat
     7201 ggaaagtcgg tatttttgga aattatttct gaattgatgg gaaactatgc gatgactatg
     7261 caggcacaaa cgatcatggt caagcaaagc caatcatcag caaacagtga tattgcacga
     7321 ttgaaaggtg cgcggttggt cacttcgtca gaaccgaatg aaggcgtccg actggacgaa
     7381 ggtctggtca aacaattgac tggtggtgat aaagtcacgg ctcggcacct ttatggaaaa
     7441 gaattcgagt tcgaacctga attcaagttg tggctggcaa cgaaccacaa gcccatcatc
     7501 cgtgggaccg atgacggcat ctggcgacgg ttaaatttaa ttccattcac tgtccaaatc
     7561 ccggatcaca aaaaggataa aaatctgaaa tttaaattgc agacggagtt gcaagggatc
     7621 cttaaatggg caatcgatgg ttgcctgttg tggcagagag agggcttgga gaaaccaaag
     7681 tcggttgttg cagcgagtca ggattatcga aacgaaatgg atcaaatagg tacatttatc
     7741 gaaacgtgtt gcgaaacagg tccaggacta aaaatatcag gcggagaatt atacaaagtt
     7801 tatcgtgaat gggcatcaga taatggcgag catactttca ctaacacgaa gtttggcaga
     7861 gagatttcaa agaaatatag caaagaaaaa gctggtggat ttatggttta taaaggaatc
     7921 acattaaaac caagaaaata cgataacgtt agagaacttt ttaaataaat ctggagggtt
     7981 ctggagggtt
//
LOCUS       DAZKMZ010000097         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000097 DAZKMZ010000000
VERSION     DAZKMZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.100"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8M5_002390"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8M5_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0359613.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8M5_002391"
     CDS             1483..1935
                     /locus_tag="ACG8M5_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359614.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8M5_002392"
     CDS             1949..2638
                     /locus_tag="ACG8M5_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359615.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8M5_002393"
     CDS             2763..5114
                     /locus_tag="ACG8M5_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0359616.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8M5_002394"
     CDS             5194..5589
                     /locus_tag="ACG8M5_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359617.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8M5_002395"
     CDS             5914..6531
                     /locus_tag="ACG8M5_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359618.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8M5_002396"
     CDS             6572..6871
                     /locus_tag="ACG8M5_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0359619.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8M5_002397"
     CDS             6905..7072
                     /locus_tag="ACG8M5_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0359620.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8M5_002398"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8M5_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359621.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKMZ010000098         7476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000098 DAZKMZ010000000
VERSION     DAZKMZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.101"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..434
                     /locus_tag="ACG8M5_002399"
     CDS             <1..434
                     /locus_tag="ACG8M5_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0359622.1"
                     /translation="MFGEETYTYTAQAMTDIETFYLPVSYYEKLARKNPEQLIYMIQK
                     ISKVVEIHELRIQIGLTSSAFDRVKQNLFILKEELGVPTKDGHITIPYPITLKELAVN
                     SGTTRETAGQVIKQLRDSGLLDYDKKRFTFCKTATTPAMRE"
     gene            complement(1043..2059)
                     /gene="tsaD"
                     /locus_tag="ACG8M5_002400"
     CDS             complement(1043..2059)
                     /gene="tsaD"
                     /locus_tag="ACG8M5_002400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0359623.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2056..2520)
                     /gene="rimI"
                     /locus_tag="ACG8M5_002401"
     CDS             complement(2056..2520)
                     /gene="rimI"
                     /locus_tag="ACG8M5_002401"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0359624.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2527..3069)
                     /gene="rimI"
                     /locus_tag="ACG8M5_002402"
     CDS             complement(2527..3069)
                     /gene="rimI"
                     /locus_tag="ACG8M5_002402"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0359625.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3053..3775)
                     /gene="tsaB"
                     /locus_tag="ACG8M5_002403"
     CDS             complement(3053..3775)
                     /gene="tsaB"
                     /locus_tag="ACG8M5_002403"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0359626.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(3966..4946)
                     /locus_tag="ACG8M5_002404"
     CDS             complement(3966..4946)
                     /locus_tag="ACG8M5_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359627.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4970..6454)
                     /gene="galT"
                     /locus_tag="ACG8M5_002405"
     CDS             complement(4970..6454)
                     /gene="galT"
                     /locus_tag="ACG8M5_002405"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0359628.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6466..7455)
                     /gene="galE"
                     /locus_tag="ACG8M5_002406"
     CDS             complement(6466..7455)
                     /gene="galE"
                     /locus_tag="ACG8M5_002406"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0359629.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2066 a         1637 c         1332 g         2441 t
ORIGIN      
        1 gaatgtttgg agaagagacc tacacttata cagctcaagc gatgactgat attgaaacat
       61 tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa aaatcccgaa cagttgatct
      121 atatgataca aaaaatttct aaagtagttg aaatacatga attgcgtatc caaatcggat
      181 tgacctctag cgcctttgac cgggtaaaac aaaatttatt cattttgaaa gaagaattgg
      241 gcgtaccaac aaaagatggt catatcacca ttccttatcc gatcacctta aaagaattag
      301 cagtgaatag cggcacgaca cgtgaaactg ctggacaagt aatcaaacaa ctacgtgatt
      361 ccgggctgtt agattatgac aaaaagcgtt ttactttctg taagacagct actactccag
      421 ctatgagaga ataacgcgaa caaaaataag cagtgaccct gataaatatc agggtcactg
      481 cttattttcc tccctaaaac ttctgaaaga tccggcagtt ttagatcact ggacaagact
      541 caaagatttt ctatgtgtgt ttcagttatg taatattcac tgtttgcaaa aaatactgtt
      601 agattaacta gatgaagcgt cttgtcctca tccgatctag attattcgat attcctcgac
      661 aataaacgat tactcagtga gggcaatcag aggaacggat aatgataaat aagctgtaaa
      721 aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa atagggcttt gacaaaaacg
      781 tgtcacagcc ctatttcgat taaacggtgt gtacctgtca actcttcggt cacaagtcat
      841 aatgttcata aacatgagga gctgtttttt gaaattgttc cttgtgaatc agagttaaac
      901 gacaggctta cgaactcttg attaaacggc gttcaaaagc ttgcatacgc ggtaataaga
      961 acagccaggt caaaaacatg agaagccgtt tttcctgggc tgttcttatt acttgatgca
     1021 gggcagcttt ttcacaacct ctttattcag ataaaaccaa gcttggatca gcattcaact
     1081 gatagctagc aaaatttctt ttttgcattt ccacatgtgc tgctgctcca atcattgcag
     1141 cattatctcc acaaagtcga agagggggaa tcactagttc tacctcaggg agtttagttg
     1201 ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc cgctacacct ccagctataa
     1261 tcagttgctt gactggataa ttttgacaag ctcgaagtgt tttagtaatc aatacatcga
     1321 tcacacttgc ttgaaagcta gctgctaaat catttttgtc tagatcttct ccacgctgtt
     1381 gtgcattatg caccaaattg ataaatgcac tttttaatcc gctaaaactg aaatcataat
     1441 tatcttcatg gatcatcgct cgcggaaaat ggtaattatc ttttccctga tgagctagct
     1501 gatcgatttc ttttccgctt gggtaagaaa gacctaatac tcttccaact ttgtcataag
     1561 cttctcctgc tgcgtcatct cttgtctccc caatgatttc atatgatcca tcttcttgca
     1621 tatagactaa ttctgtatgt cctccgctaa caagaagtgc catcaatgga aactggaatg
     1681 gtttaaccag acgggctgca tagatatgcc ctgccatatg gtttacagga ataagcggca
     1741 attggtgtgc ccaagcaaaa gccttagctg cagaaatacc aatcagcaac gagccgacaa
     1801 gtcctggtcc ataagtgaca gcgactgccg ataaatcttc tgccgacaca ccagcttcta
     1861 ccagtgcatc ttccaagcat aaggtgattt gttctacgtg atggcggcta gctacttcag
     1921 gtactacgcc accaaaacgt ttatgactgt ttatttgaga agcaacgatg ttagataaga
     1981 tctctgttcc atttctaacg accgctacac tcgtttcatc acaacttgat tcaatcgcca
     2041 gaatcaattc ttcattcatt tcatcgtctc cgtttttaac tttctgctca taatgatcgc
     2101 atcttccgtc ggattgcgat aatatgattt ccgtttacct aatatacgaa acttttctga
     2161 ttcataaagt tttcttgctg cttcattaga tagacgtact tctaaaaaaa catgcagcga
     2221 atcttttatt tgttccgtaa tcagtaattg taacaaatgc cgtgcatgcc cttttccttg
     2281 ttctgaaact gcaacagcaa tatttgtgat ctcgacttca tctattactt tcgagtatcc
     2341 aataaagcca cagatctggt tatcttcagc taaaagcaga taatcagagt gaggctgttg
     2401 gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca tgtggataag cattttcgct
     2461 aatcatccat acgcattctg ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa
     2521 ctaatctcac tcattttcta aaagttttat catatccaac gcatcttctt ttgtttcagt
     2581 gtaatatctt tttttgatcg ttgttgcttg aaaccccaat ttccgataaa gtctttgtgc
     2641 atcttgatta ctcatacgca cttctaatga catcgtctca cagcgattca ttatagcaaa
     2701 gttctcgatt tcgtcaataa gcagcgaacc gattcgttta cgctgatatg ccggtcgaac
     2761 ggcaatattc gtgatatggc aatctaaacc gaacgctctt gaaccgataa aaccaatcat
     2821 tcttccatta tcaaagatac ctagatatag atgaggaagc ggtgacttca attctgatat
     2881 aaaggcactt tttgtccatg gcaattcgcc attatagact tctcgttcga catccagcag
     2941 ctctttgata tcttctgtag tcaattctct tagtacgtaa cgacgatcga tttgttcgac
     3001 tgttttttct gtataaatca ttttatcttg aaaaaagctt ctggcatacg ctctaaattt
     3061 tttcaacata actttcgtct ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta
     3121 ctcgttttaa atagcgagga agaaaagctt gtttctctgc aactggttca actaaagcac
     3181 ctaattgagc cacagttaca ccacttggaa tatcccatgc tggaactgta ttaatcatcg
     3241 cactaggcaa tatttccttt atttcagaga agaatttgtg acaatcactc ccgacgaaat
     3301 aaatagcttc attcagcttt tgtagttgtg tgaacaaatc agaagcagaa atatgtacat
     3361 cttcaagtac agtttctaat ctattttctt tccagcggta tgccccagcg tatacatttt
     3421 ttcttcgagc atcaaacatg ggcacaataa tattatccac accgatgcaa ttagccgcga
     3481 tggttttcag actagatact cctactagct ctttatctaa tgtataagca atcgtttttg
     3541 ctgtcgttac acctatacga agccctgtat aagaaccggg accatcagaa accgcgattc
     3601 ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca
     3661 gtgttacact gtggttgcgt ttgatatttg tttgtatttg tccaagaatt tgttcgtctt
     3721 ctaccacacc gatcgccaaa gtttggtttg cagtatctat tcctaatgta atcatatatt
     3781 tgatcctttc tgctatgaca ttctcgcctt ccattgtagc atactttttt attctgagga
     3841 tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat tatctgctgg cgggcaaatt
     3901 ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc cattttccca cagcttttcc
     3961 atcttttatt tcgttgattc tcgaataatc aactctgttt ccacaataat ctttcttgaa
     4021 accggtgctt tggaattact taattgaaga atcgtctcca ctgctagctc tcccatccat
     4081 tcggtataaa cttttacagt agaaagcgag ggcgttacat atttcgctac actgacatca
     4141 ttgaagccca tcacagaaat atcttctggt accactaaac ctttttcttg aatcgctttc
     4201 agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg catcaggaac atgcggattc
     4261 gtttttaaat actgcttcac tgcttgatat cctgactcga cagaaaaatc tgcttcgata
     4321 attggacctt cgtcgtagcc tttttctgcc aaaacttgtt gaaatgccat aattctggga
     4381 tcttttagcc gctgatggtt tttcttcgta tattctgttc cagcaagcat agcgatatgt
     4441 tgcgctttat gggagacaaa gtgttcaatg actttttgaa cacttgactt aaagtcgaca
     4501 actagagagt cgattccttg agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg
     4561 gatcctttca tttctaataa ctgagcttcg tcgaattttc ctagtgcgat cgttccatct
     4621 gccggaagat cactaatgga atcccacgtt tctttcaata aagtaacgtt tgcttcttct
     4681 gccttttttt cgattcctag gcgaatagat aaataataaa gatcttcaag ctcttccgta
     4741 tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt
     4801 ttcgtataat ttaagcgttc tgctgcttca aaaatttttt gctttgtggc taagccaacg
     4861 gaaagatcag gatcatagtt gagaactctt gaaacagtag caggagaaac tcctgccaac
     4921 tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct tttttacgtt tataaaatcg
     4981 cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg cttgaatact ccagcgtctt
     5041 ccagtacacg tgcaaaaacc tgacctacag atttttgtac gacatcggtg accgtatctt
     5101 tcgtcaaatc aggatgttct ttttttaatt gttctgccca cggacgatga tattcagcca
     5161 tctggttttc ttctcctaat aagaaatgct ctacttctac tagttctggt ttcaaacgag
     5221 gtggtaaaac agcaagcccc attacttcga tcaacccaat gttctccttt ttgatatgtt
     5281 ggacatcttt atgtggatga aaaatcccat caggatgttc ttctgaaaca ttgttgtctc
     5341 gaaggacaag gtccaactca aagtaattgt cccttcttcg tgcaattggc gtaatcgtat
     5401 ggtgtggtgt accatctgct gaaaaagctc tgatagatac tgtttcatcc gaatatgctt
     5461 tccatttttc caatactgag ttagctgctt caataagcaa gtttttctct gttgattgca
     5521 aacggatcac agacatcggc catttcacga ctcctgcgat gacttgcgga taatcttcta
     5581 gtttgataag tttttctatc ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat
     5641 aatgatcatg agaaagaatc gatcctccaa caattggtaa gtcagcattc gaaccaacaa
     5701 agtaatgagg caagacttca gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt
     5761 tcatcgggcg gtgttgttct gataaaatga tcgaatgttc attgtaataa gcataagggg
     5821 aatattggaa tccccagctt tcaccgtcta gattcattcg aataatccgg tgattggtgc
     5881 gggctggata gttcaagcga cctctatatc cttcgttttc catgcaaagc atgcaggtag
     5941 gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg ttttggatct ttttctggct
     6001 tagataaatt gattgtgatt tccagttcac catactccga ctctgccgga aatttgatgt
     6061 tttttgcgat ggctcttgtt ttgatgtagt catttttctt acttaaatta aagaaatact
     6121 cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag
     6181 gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat ctcttgttct gctggcaatt
     6241 ctgaaagaac accattttct cttgccttag cgacaagctg atccaataag tctacagatt
     6301 cttccttaac tggtcttact tcaatgcttt ctagagcatc ttctcctacc atccctaaaa
     6361 gacgattctg taaatataag cggtccattt ccatccatcc gccagcttcg atcgcaagtg
     6421 tggcaaaatc acaaatcgtt tgactgattg acatatctca ttcccctatt cttcataacc
     6481 atttggatgg ctgacatgcc aatcccatgc tgttttgata atcgctttga tatctgtgta
     6541 ttcaggtttc cagcccagaa tctctctcgc tttttcactt gaagcgacta atcggctagg
     6601 atctccagct cttcttggtg ctatttctgc tggaatttct tttcctgtca cttctctagc
     6661 tgcttctagc atttccttga ctgagtatcc tttgctgctg cctaaattga agaagttgct
     6721 ttcatttcct tctttcaagt attccaaagc taaaatatgt gcagcaatca aatcttctac
     6781 ttgaacataa tctctgatac atgttccatc tggtgtatca taatcgtctc cataaacagc
     6841 caaggctttt ctttgaccaa gagctacttg taaaatgatt gggacaagat gcgtttctgg
     6901 tgtatgatct tctccgatag atgcatctgc ttttgctccg gctacattga agtagcgcaa
     6961 agcgacatag cgcatgccat atgcttggtc acaccacttc atcatttttt ccatcatcaa
     7021 tttactttca ccatacggat ttttaggatt agctggtgta tcttctgtga ttggagactc
     7081 tttcggctct ccataagttg ctgcagtaga agagaatacg attttattca cgttgaactc
     7141 gtgcatgact tcaagtaaga tttgcatacc gtatacgttg ttgttgaaat acatcaatgg
     7201 tttttcgaca gattctccta ctaaagagct tgcagcaaaa tggattactc cttcaatcgg
     7261 ttccttttca aatacggaac gtagaaattc tttatcccgg atgtctcctt cataaaaatg
     7321 agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa ttatctacca cagctacttg
     7381 ataccccttt tgaactagct ggtctacagc gtgcgaaccg atataaccgg ctccgcctag
     7441 tactaaaatt gtcataaaaa acactccttt gattag
//
LOCUS       DAZKMZ010000099         7416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000099 DAZKMZ010000000
VERSION     DAZKMZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.102"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..2724
                     /gene="clpB"
                     /locus_tag="ACG8M5_002407"
     CDS             115..2724
                     /gene="clpB"
                     /locus_tag="ACG8M5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0359630.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2875..3561)
                     /locus_tag="ACG8M5_002408"
     CDS             complement(2875..3561)
                     /locus_tag="ACG8M5_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359631.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3539..4033)
                     /locus_tag="ACG8M5_002409"
     CDS             complement(3539..4033)
                     /locus_tag="ACG8M5_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0359632.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4053..5273)
                     /locus_tag="ACG8M5_002410"
     CDS             complement(4053..5273)
                     /locus_tag="ACG8M5_002410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0359633.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5420..7255
                     /gene="lepA"
                     /locus_tag="ACG8M5_002411"
     CDS             5420..7255
                     /gene="lepA"
                     /locus_tag="ACG8M5_002411"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0359634.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
BASE COUNT         2452 a         1405 c         1508 g         2051 t
ORIGIN      
        1 aaagtcaaaa aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt
       61 tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat
      121 attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc
      181 actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat
      241 cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa
      301 attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa
      361 aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc
      421 ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat
      481 ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc
      541 gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca
      601 ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc
      661 atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat
      721 aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct
      781 caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta
      841 gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa
      901 gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc
      961 cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa
     1021 ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt
     1081 caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa
     1141 ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac
     1201 catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat
     1261 attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg
     1321 attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg
     1381 cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc
     1441 ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg
     1501 cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat
     1561 aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta
     1621 ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca
     1681 aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc
     1741 gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta
     1801 attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg
     1861 gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt
     1921 tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct
     1981 gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa
     2041 aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt
     2101 ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc
     2161 gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg
     2221 actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat
     2281 atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg
     2341 gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc
     2401 gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa
     2461 atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat
     2521 gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg
     2581 aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat
     2641 gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca
     2701 gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg
     2761 acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc
     2821 gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt
     2881 atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga
     2941 ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt
     3001 atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag
     3061 attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag
     3121 tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc
     3181 atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac
     3241 cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa
     3301 aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag
     3361 tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc
     3421 cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt
     3481 atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt
     3541 aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg
     3601 aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa
     3661 attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct
     3721 gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt
     3781 ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag
     3841 ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt
     3901 cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga
     3961 tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa
     4021 aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc
     4081 atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg
     4141 attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata
     4201 tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc
     4261 catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga
     4321 aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc
     4381 cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct
     4441 aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa
     4501 ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga
     4561 aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag
     4621 tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa
     4681 tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg
     4741 tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc
     4801 tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga
     4861 tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta
     4921 tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt
     4981 ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga
     5041 tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact
     5101 tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg
     5161 atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt
     5221 tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca
     5281 tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct
     5341 gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta
     5401 tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg
     5461 taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt
     5521 agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga
     5581 tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc
     5641 gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac
     5701 ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc
     5761 acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga
     5821 gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca
     5881 agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc
     5941 agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg
     6001 agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg
     6061 tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact
     6121 gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt
     6181 tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt
     6241 ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt
     6301 accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc
     6361 tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca
     6421 attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact
     6481 tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac
     6541 gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga
     6601 taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa
     6661 agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa
     6721 acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa
     6781 tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg
     6841 ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga
     6901 catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc
     6961 ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt
     7021 tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc
     7081 cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact
     7141 cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc
     7201 gcaagaagcc ttcatggccg ttctgaaaat ggatgagcaa gatagtgtga aataacttat
     7261 aatcaatcga aaaacctatt aaataaacct ttttgagtag atgcaacact gaggattatc
     7321 tgtataaaag attttaaact gtcatttggt tatcacggcg atactaaaat taacaaaatt
     7381 ggaatatttt tcttgaagat ttttagctgt cagtat
//
LOCUS       DAZKMZ010000100         7409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000100 DAZKMZ010000000
VERSION     DAZKMZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.103"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1699
                     /locus_tag="ACG8M5_002412"
     CDS             113..1699
                     /locus_tag="ACG8M5_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0359635.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(1765..3540)
                     /locus_tag="ACG8M5_002413"
     CDS             complement(1765..3540)
                     /locus_tag="ACG8M5_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0359636.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3751..4653)
                     /locus_tag="ACG8M5_002414"
     CDS             complement(3751..4653)
                     /locus_tag="ACG8M5_002414"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0359637.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4932..7277
                     /locus_tag="ACG8M5_002415"
     CDS             4932..7277
                     /locus_tag="ACG8M5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0359638.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         2290 a         1451 c         1433 g         2235 t
ORIGIN      
        1 accagcaaga cacaccacga aaaaaaatta ggcataccga atctttttct ttaatttttt
       61 ctttctgagg tgtagaatgt aatcgtagat aaataggaaa ggtggttttt tcttgaataa
      121 tcaatccaac aaacaatttc ataagcatcg acttattgaa catacaaatg gtttatcttt
      181 agctgaagta aatggaacag tggatgtacc aaagggaaaa agttttttta aaacacttct
      241 tgcttactct ggaccaggtg cattagtagc agttggttat atggatccag gtaactggtc
      301 cacttcgatc actggtggac aaaatttcca atatttattg atgtcagtta ttttgatgtc
      361 tagtctaatt gctatgcttt tacagtatat ggcagcaaaa ttaggaatcg tgacccaaat
      421 ggatttggcc caagcaatcc gagcacggac aagtaagaca ttgggtgtca ttttatggat
      481 cttgactgaa ttagccatta tggcaacaga catcgcagaa gtcattggtg cagcaatcgc
      541 cctctactta ctttttgata ttccattggt gattgccgtc tttattactg tattagatgt
      601 tttcgttctc ttactactga caaaagtcgg gtttcgtaag atcgaagcac tggttgtctg
      661 cttaattttc gtcattttat tcgtttttgt ctatcaagtt ggcttgtcta acccagattg
      721 gcacagttta tttgctggct ttatccctaa cagtcataca ttctcagact caccggtcat
      781 cggcggcgaa actccgctga ctggagcttt agggattatt ggtgcaacag tcatgccgca
      841 taacttatat cttcattcag cagtttcaca aactcggaag atcgatcacc aaaacgaaga
      901 agatgtagca caagctgtac gtttttctac ttgggattct aatatccagt tgtcaatggc
      961 ttttctagtc aatgcactgc tattgatcat gggtgtcgct gtcttcaaaa ccggttctgt
     1021 ccaagatcct tccttttttg gtctgtatga agctttatct gatccatcag ctatgagcaa
     1081 tggtattttg attgctgtag caaaatctgg tatcttgtcc actttatttg ctgtagcact
     1141 tcttgcttct ggccagaact ctactattac tggtacgttg accggtcaag tcatcatgga
     1201 aggattcatc catatgcgta tcccgatttg gctaagaaga ctggtcacac gattgctttc
     1261 tgtgattcct gtattgattt gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca
     1321 tgccgctttg aataacttaa tgaatgaatc ccaggtgttc ttggcttttg cgttgccatt
     1381 ttcgatgatt cctttactga tgatgaccaa cagccaagcg gagatgggta gccgatttaa
     1441 aaattcattc atcgtaaaac ttcttggctg gttctctgtg attagtctaa cttacttgaa
     1501 cttacgagga ttaccaggac agatcgaaag tttcttcggc gagcaagcca cttccgctca
     1561 actagcattg gctgatcaca tcgcttatgt aatcattgtt cttgtccttc tcctgttggc
     1621 atggacgatt ggtgatttat ataaaggaaa caaaaaatat gaacagcttt tgcaggaaac
     1681 ggtagaagaa atcgaataaa aactgatttt tccacacaaa gacccttgat agttaggtta
     1741 aaaatctagc taccaagggt ctttttattt agaagataaa gcttttttca gatttaattt
     1801 actgaaaact agcatgcttg ttactacgat gaattgcgtt aatggcacgc ctgcaccagt
     1861 gtagacactt aaaaaatagt cctttttttc tggcgcataa gaacaaaacc atgagacacc
     1921 gaaaagaaaa accaattgtg tcagtattaa tccggtgacc acaatcaaag aggaactata
     1981 ataggcacga tcagcttttg caaatacggt tcctagtatc ataagtaata tccctgctat
     2041 tgaagcaatc agcacgggaa tagccagaac ttggtaaatt tttatgatga cattcgcgac
     2101 ttttgctgat tttgttaatt ggaaattagg tgttctcggt tcattactat cgatccaatt
     2161 ctccattagc ggaaggttta ataattcttg agcctgtttg acttgttctt gcgttccgaa
     2221 actataattt cctccttgat ggaaatcttt atatcgccat gcataagcgt atcctgattg
     2281 aaggaaatcg aatacgattg gcagatcact catcgtcttt ccatctcctg atcccatcag
     2341 atagaactct tttttctttt tgatggttcc cttgtgatag gcttcttcta attcttggtt
     2401 gacttttttc caaaaatcat tggtttcttt tgcattgttt tggtaataac cagattcgct
     2461 taccacatct cgcaatgccc aaaagatgat atctccgggt aattctttta cttttccacc
     2521 ttgagtccac gcgtgagtcg tatataattc gtcaatacgg gtagaaacag tatggaatgt
     2581 aggagatgcg gctaatgctt tatttagtgc agcatgtgag acccatatct cactgttttc
     2641 tttattcaag ttcgtttcat catctattcg gatcagttgt ttcatcaaac gtccaaattc
     2701 tccactggaa cggtcattga tggttgcaat accgtatgtt gtctcatttt tatattcgat
     2761 ccactgatag acgatggaaa agccaactat aggaagtaaa aacaatgaaa gtttcagcca
     2821 ttcccgtttc gagagaagaa agagtcgttt ttttaacttc tttacttctt tgaaagaaaa
     2881 gtcttgccct agcaagatct ggacgaaact gatacccaaa gctactacag taaaaggaag
     2941 aagccagata gaatcttcgc gaatgaacca aaagaaagga aaagcgacag aaagccctaa
     3001 aaaccatggc agcaattttc tgctagaacc gtatttgcgg taataaatag ctaataaaca
     3061 agcgataatg atctcaacag ccggaatcac gagggcatta cgatatgttc gagtggaata
     3121 ttcatgtgta aaactaatcg gagaaaacaa aaaataaaaa tagatactta aaagaagata
     3181 cttgttcttc acaatcggtt taaacgcgag tgcaaccaat atactggcaa aaatactgaa
     3241 catgccgaac aaaactgcat aagaaagatg cagtttatgt gcaagtacta aaaataagct
     3301 gtaggagatt cctttggaaa gtgttgtagt cgtatactcc cctagccatt taccatccaa
     3361 aatattttga gcatagtgaa tcagcagctg atcatcatag gtcgccgaca tatctgttcc
     3421 atagattgct ttactatata aatacaaccg aataattgta aataatacgg ctatgccaag
     3481 aataaagtat gtatgtttct tttttagttg gtcgatcaag gggtttaaaa tgttcagcat
     3541 cttttcgtcc tttcaatcac gttttgctac ttgtttgtga aacttttcgt tttttacttt
     3601 tctatcagta taccctgttt gtctgaattt aaaaagaggg ggcaaaagga gcatggcgaa
     3661 aacgaatgca aaaagcactc ctttgtttct taaacaaaaa aggacaaggc agactgccca
     3721 atcctttttt gttgctgtca ggtagaaaac ttaaagtgtg acttcttctg ctaataactc
     3781 agggaacact tcgggatcga atgcttgcgc tttgaacatg gaaatttctt ttccatatgg
     3841 tgcgtaagct ttctttttat ctttaccgac atatggggtt tctaatattt tcgaaatgtt
     3901 gtcaaacatt ggatcatgga cgattttgtt caatgtgtca aatccaattg ttccaaaacc
     3961 gatattggca tgccgatctt tatgagaacc ttgtggattt ttagaatcat tgatatgcag
     4021 tactttaagc cgatctaatc cgatgacctt atcaaactct tccattacac ctgaaaaatc
     4081 ttcttttata ttatatccgg catcattcgt atgacatgta tcaaaagtaa cggataattt
     4141 ttcattcaat tttactccat cgatgatttg cgctaattcc tcaaatgttc gaccgatttc
     4201 cgtacctttc cctgccatcg tctccaaagc gatctgtgcc gtctgttctt ttgtcaaaac
     4261 ttcattcaac ccctcaataa ttctagcaat cccagcttct gcccctgctc ctacatgagc
     4321 gcctgggtga agggtgattt gagtagcacc taaagcttcc gcacggacaa tctcttctct
     4381 cagaaattgt acagcaaatg gaaacatttc tggtttgatc gtatttccca aattaatgat
     4441 ataaggcgca tgaacgacga tatttgttag tccgttttct gccataaatg cttttccggc
     4501 atcaatattc atctcttcga tagcttttct tctagtattt tgaggagcgc ctgtatagat
     4561 cataaaggtc gaagatccgt aactggctgc ttcttctgcc gcgcctaaca gcattttctt
     4621 tccgctcatg ctgacatgtg agcctaataa catggatgga ttcctccttg aaaggcaatt
     4681 gtttcgaaca attcctgtat attgataaga tgatgtacaa agaaaatggt ttgtttttct
     4741 ttgccctgtt ctccatagta actgaaactt tccctaaaga aaagcttaag aactctttca
     4801 taaaaaagat catttcctca cgttttatct cattcgaaaa agaaattcct gtcaaaaaca
     4861 gccttgtctg tttttttgtc ctattatttg ctataattag cgaaataaaa ccattagttg
     4921 aggtgaccct tttgccacgc aaaaatactc gaaaaaaaca gaatagaaga aaaaaagaaa
     4981 aatggtttgt tcctaagatt atttttagag tgtttcaatc attgactgtt tttattactg
     5041 ttcttctttt gttgttcgca gctctaggta tcggaatagg tgctggctac tttgcctatc
     5101 ttgtcgaaga cacacaattg ccgactaaaa aagaactgca gacagaacta ggaaacatta
     5161 cggaaacgtc aaagattgtt tatgcagata atacagagat atcaaaaatc cagacagatc
     5221 tgatgcggac gactatttca tctgataaga tttcgccttt attgaaaacc gcgattatca
     5281 gtacagaaga tgaatacttc gataaacatc aaggttatgt ccctaaagct gttttacgag
     5341 cactagtttc agaagcgact ggcatcggct cgtctggagg atccactttg acacagcaat
     5401 tagtcaaaca acagatccta accgatgaaa caacattcaa acgtaaagca aacgagatcg
     5461 ttctttctgc acaagtagaa aaacactttt caaaaaatga gatcattact acgtatttga
     5521 atgtctcacc atttggtcgg aataataagg ggcaaaatat tgctggagtc caggaagctg
     5581 ctagaggaat ctttggtgta gatgcaaagg atgtgactct gccacaagct gcttatatcg
     5641 ctggtttgcc tcaaagccct atcacgtata gtccatacac gaatacaggc gcgctaaaag
     5701 aagatctttc tgccggtctt gccagaaaag attttgtttt attcagtatg taccgtgaag
     5761 gacaaattac gaaagaacaa tacgaagaag cgaaagccta tgatcttaca aaagactttc
     5821 tcccacaaca aatcgcggaa cagactgaca gagaattctt gtattatacc gtgatgaacg
     5881 aagcaacaag gattattgca caacaattgg cagaaaaaga taatgctgat atgtcagatt
     5941 catcggtcag tgaagcttat tatcaaaaag cacagcaaac gatccaaaac aaaggatata
     6001 cgatccattc gacgatcgat aaagatatct atgctgcatt acaaaccggc gtagaaaatt
     6061 atggctacat gttagatgat ggtgctggta gccaagtgga gactggaaat gtattgatgg
     6121 ataacagaac cggacgtatc tatggcttcg tgggtggtcg gaattacagc cagaatcaaa
     6181 ataaccacgc ctttgataca gaacgtcaag ctggttcatc catcaaaccg gttcttgttt
     6241 acggtcctgc aatcgatatg ggacttgtcg gatcagaatc ccgtgtatca gattatgcaa
     6301 cgacttggca agaaggaaaa aatgctggtg agaaaatcgt caatgcgacc aatgaagggt
     6361 cgaacacctt ccaaaccgtc cgtgagtcac tggaatggtc caacaatatc ccagcttatc
     6421 acttatatca agatgtgttg aattccggtg gatcgaaaca atacgcttac gagcactact
     6481 tggcgaagat gaactaccca gcaaatgata attggggcgt tgaatcggct cctttaggga
     6541 cagtagatgt aacgacgctt caacagacaa atggattcca ggccttagca aatggtggag
     6601 tctacgaaga aggctatatc attgattcca tcacagataa tgcaggtaat gtgatttata
     6661 aacatgaaag taattctgtc cgtatttatt ccgaagcaac ggcatcgatc atgaatgaca
     6721 tgatgcgtag tgtgatcaat gaaaaaatca ctacaccatt caaagatgtc atctcaagct
     6781 taaacggaaa tctaggaaat gcagattggg tcggaaaaac tggttctacc aatgaatacc
     6841 gagattcatg gctgatcgtc tcaactccat ctatcactat cagtagttgg gcaggacacg
     6901 atgacaatac tggaatggac agccaagcaa gaatacgttc ggctaattat cttgccaatc
     6961 taatcaatca agcttaccaa gcaaaccctg agattttcgg cactgatgag aaatttgact
     7021 tatcatccga tgtgatgaag aaaaaagtat ctgggttcac aggacaaaca gctggaaaag
     7081 ttactgtcaa taaaaaatcc atccaaacgc caggaaaaac agtggagtca ctttgggcaa
     7141 aaaatggtcc gaagaaaagc gcctttaaat tcggcgtagg cggtactgat gaaaactata
     7201 cagattactg gaacaccgct tcagcttatg ctagagcgaa tccagagacc aaagaatcag
     7261 acgataaaga tgattgatct aatagattaa tcagaattaa agggagctgc gacaaaaatc
     7321 ttgtcacagc tccctttttc cgaataaatg gtgttcaaaa gcttacatct acgatattaa
     7381 gacaaattta tgtaaaaatg tgaaaaacc
//
LOCUS       DAZKMZ010000101         6954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000101 DAZKMZ010000000
VERSION     DAZKMZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.104"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            266..862
                     /locus_tag="ACG8M5_002416"
     CDS             266..862
                     /locus_tag="ACG8M5_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0359639.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(920..1402)
                     /locus_tag="ACG8M5_002417"
     CDS             complement(920..1402)
                     /locus_tag="ACG8M5_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359640.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1508..1705)
                     /locus_tag="ACG8M5_002418"
     CDS             complement(1508..1705)
                     /locus_tag="ACG8M5_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359641.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2000..2758
                     /locus_tag="ACG8M5_002419"
     CDS             2000..2758
                     /locus_tag="ACG8M5_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0359642.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2818..3246
                     /locus_tag="ACG8M5_002420"
     CDS             2818..3246
                     /locus_tag="ACG8M5_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359643.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3292..3364
                     /locus_tag="ACG8M5_002421"
     tRNA            3292..3364
                     /locus_tag="ACG8M5_002421"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3325..3327,aa:Gln,seq:ctg)
     gene            complement(3514..3885)
                     /locus_tag="ACG8M5_002422"
     CDS             complement(3514..3885)
                     /locus_tag="ACG8M5_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0359644.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4100..4285
                     /locus_tag="ACG8M5_002423"
     CDS             4100..4285
                     /locus_tag="ACG8M5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359645.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4368..4556)
                     /locus_tag="ACG8M5_002424"
     CDS             complement(4368..4556)
                     /locus_tag="ACG8M5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359646.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4580..4843)
                     /locus_tag="ACG8M5_002425"
     CDS             complement(4580..4843)
                     /locus_tag="ACG8M5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359647.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5001..5258
                     /locus_tag="ACG8M5_002426"
     CDS             5001..5258
                     /locus_tag="ACG8M5_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0359648.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5329..5520)
                     /locus_tag="ACG8M5_002427"
     CDS             complement(5329..5520)
                     /locus_tag="ACG8M5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359649.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5661..5981)
                     /locus_tag="ACG8M5_002428"
     CDS             complement(5661..5981)
                     /locus_tag="ACG8M5_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359650.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6004..6438)
                     /locus_tag="ACG8M5_002429"
     CDS             complement(6004..6438)
                     /locus_tag="ACG8M5_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0359651.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6773..>6954
                     /locus_tag="ACG8M5_002430"
     CDS             6773..>6954
                     /locus_tag="ACG8M5_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0359652.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2175 a         1359 c         1067 g         2353 t
ORIGIN      
        1 ggcaatatca taaaaaaaag aagatagaga tttaataatt atatatcagc tgtttttttc
       61 aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa atccttacat
      121 aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg agcatatctg
      181 tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa
      241 cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga aaaaccgccg
      301 ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa caactttgat
      361 caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac gcgcagttat
      421 ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac ttcgcccatt
      481 gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca aaaatggtta
      541 tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag aacaatttga
      601 attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg caacagctaa
      661 tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt acaacccagt
      721 tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac tacgttcgca
      781 attagtattc ggttcaccag aaacaccggc tgaggaaaaa gaatacatga acgatgccga
      841 tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg tgacaaacat
      901 cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct gcctcatcgt
      961 ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat ttcagcatcg
     1021 gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct tttggctgat
     1081 ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa
     1141 caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa atcattgctg
     1201 acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta taccaaatga
     1261 ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca gtccgataaa
     1321 taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat aaaaacaata
     1381 gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca gaaaatagga
     1441 aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt
     1501 gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt tcatctaata
     1561 attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt gtcatttttg
     1621 atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca tattgttctc
     1681 ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac
     1741 attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca gttaaaaatc
     1801 aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag ctgttgtatt
     1861 tagaagaatt tttttcaaaa aattaaaaca tattccttaa aaactaatta tttcctacat
     1921 attaaaatat aatacttcca tttccaataa aaatacattt ataataggaa agataaacag
     1981 ctgaaaaggg agatttcaaa tgaagaaaaa aacgttcaaa aaactattgt ttctaggggg
     2041 cacagctgct cttttattaa gcggtacaag ttattaccta catatccatt ctccagaaca
     2101 tacaatgaac actgcacaaa aaagttcaac acaaaaaacg aaaaagacaa aagagaaagc
     2161 agaagtttta ctagatgttc cattagagag ccagtttgat tctccttctt tagaaaatgg
     2221 ctgtgaagtc acttcgcttt ctatgctttt atctttttat ggttatgaaa caactaaaaa
     2281 ccagttagct gagcaattgg attacgtccc tgttttcaat gcagatggga ctcatggcga
     2341 tcctaatgaa ggatttgttg gtgatatcag tggcggagac tgggctatgg tcgtctatgt
     2401 tcccccagtt gcctcccttg ctcagcaaat cgtccaaacg gattatcaca cttttccaaa
     2461 aaccgatgct caactcgatg atatcaaaaa agcactatct aaaggaaatc ctgtctggac
     2521 ttctgtgact atcaattttg aagtcccaga tgaaactgat tttatgactt ggaccacaaa
     2581 taatggagaa gtaagagtca ctcctcttgt tcatgcttgt gtagtcactg gatatgacaa
     2641 agaaaatatt tatgtcaacg atccctatgg tgtaaaaaat cgagcagtac cgatcaacga
     2701 tttttcggcg attttcactg caatgggtgg acagatgcta actttggaga aaagctagta
     2761 attcaaacaa aaatccctta tactcaagat accaaacctt tgaagggagc gtagataatg
     2821 gaaaaattca cacataaaaa aatggatcca aatgaaattc cgattatttt tgtccgtgac
     2881 tgcaagggaa atgttcaagg taaagtctct attaatgagt ggaacgagcg tcgcagacct
     2941 gctaccctca atgaactgga aatcaaattg tatcgtcaat ctcttgttta ttacgcagat
     3001 caagagtacg agaaagccac cgatttattg aaatttctaa ttgctcgaac agagtatact
     3061 cgctttgaat atattgaacg tttagcaaac atctatcata ttatgaatga acctgtcaaa
     3121 gaatatcagt tattggacac cgtcttgtca gtagcagaac tgattgccct tcctgctgga
     3181 ttagaaaaga aactagtacg ccgcctttta cgagtaaagc aacaactatc cgatcaagaa
     3241 aaataacttg cgttttttta ggaaagtgct atgattattt tgttgaataa atgggatgta
     3301 gccaaactgg taaggcactt gactctgaat caagcaattg taggttcgaa ccctgccatc
     3361 ccaatttgat gtgcttgctg aaaaaatgaa ccccctgtaa aagaacaatc tttttgcagg
     3421 gggattattg tattattttt actacttgta tttagtttac ataattaaat tatgaataat
     3481 aaaatcgggc gtattcttaa tacaaaaact gatttaccca ataattgcta atgctttctc
     3541 cggaactttt tctctaggaa cttcctcata cgtttctatg tgtgctcctt tattgctaat
     3601 tttcaaataa tgattttctt ttaacacaga aagaccatta aattcaaccg ttcgttcttt
     3661 cccattggca tcagcagctt tctgcacata ccttgctgta ccatattcat ttacttcctc
     3721 tggtttttgt gtctttacat agacctctcc tttttcaact aatggattta gctgatcaag
     3781 tataccaggg atttctccat aacttccttc agtataatat ttcagtccaa atagcaaaat
     3841 accactaatc gccgccagtc caatcaaata tttaatcact ttcatctttc tacctccttg
     3901 ttatatattc agtatatggg tcagttaaag aaattccatc cgtcccaagt tggagatttt
     3961 tctcctactt tagaagcgag aatcgagtgt ttttttagta aaaatgatcg ttatttgata
     4021 ttattgacgt gtggataaat cagtttttct cgttttgtcc atgtaaaaaa tcatgttgat
     4081 agcaaaggat gtgttcaaaa tgaatcaaga agaattattt caaaaagtca aggaaatgat
     4141 taagaacggc aatttcgatg gcgcaaaacg atttatcgaa gaacataaag aacaattagg
     4201 accttataaa gagaaagccc aaaatctttt aaaagatgta aatatcgaca gtgtcaaaaa
     4261 caaatttaaa aatctattca aataagtaaa actttcactt ttgtaaaatc aaaggaggac
     4321 aatgctgctg catcgtcctc ctttggttct tttctgaata cttcatacta aaataaacgt
     4381 gtcggatcgt aatgataagt cacattgccg ttcagtgagg cttttcccac cacatattta
     4441 cgattctcca acttcagcca cgcttctcga aaagccatta attcttctct ttttacatca
     4501 atcttttcga tttttccatc gatcaaatct tgaataagtt gttcatatgg attcattttt
     4561 ttcctccatc tgctgtcaat caataagttg taaaaatagg ttcatatgct tttttggtat
     4621 ctagatcttc tctgactttg actgtcgtat aaataatttt ttctactggg atactataat
     4681 aacttccttc accattattg aatcggtgaa cgtctttctt tgctaaaata ccatttattt
     4741 tattcgtcag tacacgttgt tcgacactgt tacttacaac atagtaaatc acccgaatat
     4801 tattgatcaa atgcaaatcg atttctaaca ctacatgatc catgaaaatc ccactccttc
     4861 tttcactttg attatagcaa gtttctcctc taatttcaaa atcgatacag attttaccgg
     4921 ataaatccac aaaactgctt gcgccatttc gcacaaagtg ttattttgta tcatggactt
     4981 tatttatcgg aggcaatagt atgatcgcga tcggtcaatt tgttttttat attccttttt
     5041 ttattatgat aagcattctt ttctattaca taaaatggac aaaaaagaag ttttcagttt
     5101 tactggcctc tttacctgct gtatatttta cttatcaaat tttttctttt aggcattggg
     5161 aaacaacgtc tgtattaata acacacatca tcgaattgac gctttcagta atttttctta
     5221 ttatttggat ctatttttta tataagaatc agaattgaat ccctcgcatg tttaaaaaga
     5281 aaacgattaa agatgcagca aatttctgcc gatctttaat cgttttttct attctgttat
     5341 accaaaaaaa tgtttgaatc ggtgaaggat cgttccttgt tttaattcta taaattcttg
     5401 tgcgatcggg tacttttctt ccaaattttc tttgttcttt tcgatcaggc ttttcgcttc
     5461 ttcatatttt ttatctgcac atagtgcttt tacttcctgt aatacttttc ctgcttccat
     5521 ttgcctcacc ctcttgccat tttattgctt taagttttat ttaagttata cgcattattc
     5581 tagtttcatc ccaaataacc ttttttcatt tttactcctc gaactaatca gttttctaaa
     5641 ggctgattgg ttcgtctttt ttatttttcc tccatttcat tctgctcttc tgttaattta
     5701 tctaatgctt tgattgatgc tgcggctctt gcataatcat agctgtataa ttccataaat
     5761 ttatcaggca gaacaaacca cgctggatga taaccgttag ctgtgacagg gaatccttta
     5821 tcttgttggt tatctccatc ccatctcaga gcaacacctt tttttccttt ccactttact
     5881 aaagcgatcg agtatccctt ctttgtttgt gtgccgtctt caataattgc taaaatttct
     5941 attttttctt ttggcgcacg gacttcttga ggacgaacca ttttctattc ctcccttatc
     6001 tcattacttc acgaccaaca cactgcaagg ggcatgtgat acgacataag aagcggtcga
     6061 tccaactaat gcttgttgta tagcaccttt tcctgtcgct cccataacga tcaaatcaat
     6121 attttcttgt ccagggatga cattggcaat caaatttctt ggatctccag tttcaactat
     6181 tgcgtggatc tcagcaatgc cgtactcttt ggcatcattg atttttttca gcatggctac
     6241 ttctgttctt tgtttttcat ctgcatagat ttttgaaaag gcataggcac tatgggtcag
     6301 ctcaactttg ttgatgattg acacgataaa taagtgggct tcattgtcct ttgctaagtc
     6361 aagggcttca gcgaacgctt tgatcgattg tcttgatccg tcaactgcta ccatgatgtt
     6421 ctggtatctt ttactcattg gaaccgcctc ctttttcttt tattgtaaca tctcaagcaa
     6481 aaatacaaaa aatatacgca atatgtaaat aaaatgatga acaaaagcaa tttctttcaa
     6541 gttatttata catttctttt ctcatcagaa cacctgttct aatcccatta tttctgcatt
     6601 atgtgaaatt cctcatcatt tttcacatcg agcgaacatc attctgttac aaaaaacgtg
     6661 gatttgtcat aaattgtcat gcttctttta cctatgaaat attttcacgt gttaaagtat
     6721 tcctcgttga acacaacaaa taaaactttt taacgaataa aggagatttt tcatgacatc
     6781 acttaaaact ttactttttg gaacaacttt ggctgccggc gctgcattct tcatgggaac
     6841 gactgctcat gcggacgaag cttataccgt tcaatcaggt gatactctat caacgatttc
     6901 tcaaaaatac gttggtgata actccttgat caatgccatt gcagaatcaa attc
//
LOCUS       DAZKMZ010000102         6808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000102 DAZKMZ010000000
VERSION     DAZKMZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.105"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..592)
                     /locus_tag="ACG8M5_002431"
     CDS             complement(134..592)
                     /locus_tag="ACG8M5_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0359653.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(605..1936)
                     /locus_tag="ACG8M5_002432"
     CDS             complement(605..1936)
                     /locus_tag="ACG8M5_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0359654.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1947..3389)
                     /locus_tag="ACG8M5_002433"
     CDS             complement(1947..3389)
                     /locus_tag="ACG8M5_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359655.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3376..4140)
                     /locus_tag="ACG8M5_002434"
     CDS             complement(3376..4140)
                     /locus_tag="ACG8M5_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0359656.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4244..5119)
                     /locus_tag="ACG8M5_002435"
     CDS             complement(4244..5119)
                     /locus_tag="ACG8M5_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0359657.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5374..5458)
                     /locus_tag="ACG8M5_002436"
     tRNA            complement(5374..5458)
                     /locus_tag="ACG8M5_002436"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5422..5424),aa:Leu,seq:caa)
     gene            complement(5476..5547)
                     /locus_tag="ACG8M5_002437"
     tRNA            complement(5476..5547)
                     /locus_tag="ACG8M5_002437"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5513..5515),aa:Gln,seq:ttg)
     gene            complement(5557..5629)
                     /locus_tag="ACG8M5_002438"
     tRNA            complement(5557..5629)
                     /locus_tag="ACG8M5_002438"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5594..5596),aa:His,seq:gtg)
     gene            complement(5691..5775)
                     /locus_tag="ACG8M5_002439"
     tRNA            complement(5691..5775)
                     /locus_tag="ACG8M5_002439"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5739..5741),aa:Leu,seq:caa)
     gene            complement(5796..5866)
                     /locus_tag="ACG8M5_002440"
     tRNA            complement(5796..5866)
                     /locus_tag="ACG8M5_002440"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5832..5834),aa:Cys,seq:gca)
     gene            complement(5912..5983)
                     /locus_tag="ACG8M5_002441"
     tRNA            complement(5912..5983)
                     /locus_tag="ACG8M5_002441"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5949..5951),aa:Gln,seq:ttg)
     gene            complement(5995..6067)
                     /locus_tag="ACG8M5_002442"
     tRNA            complement(5995..6067)
                     /locus_tag="ACG8M5_002442"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6032..6034),aa:His,seq:gtg)
     gene            complement(6070..6143)
                     /locus_tag="ACG8M5_002443"
     tRNA            complement(6070..6143)
                     /locus_tag="ACG8M5_002443"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6109..6111),aa:Trp,seq:cca)
     gene            complement(6150..6230)
                     /locus_tag="ACG8M5_002444"
     tRNA            complement(6150..6230)
                     /locus_tag="ACG8M5_002444"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6194..6196),aa:Tyr,seq:gta)
     gene            complement(6232..6307)
                     /locus_tag="ACG8M5_002445"
     tRNA            complement(6232..6307)
                     /locus_tag="ACG8M5_002445"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6272..6274),aa:Phe,seq:gaa)
     gene            complement(6343..6415)
                     /locus_tag="ACG8M5_002446"
     tRNA            complement(6343..6415)
                     /locus_tag="ACG8M5_002446"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6380..6382),aa:Asp,seq:gtc)
     gene            complement(6425..6497)
                     /locus_tag="ACG8M5_002447"
     tRNA            complement(6425..6497)
                     /locus_tag="ACG8M5_002447"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6462..6464),aa:Val,seq:tac)
     gene            complement(6513..6584)
                     /locus_tag="ACG8M5_002448"
     tRNA            complement(6513..6584)
                     /locus_tag="ACG8M5_002448"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6549..6551),aa:Glu,seq:ttc)
     gene            complement(6599..6688)
                     /locus_tag="ACG8M5_002449"
     tRNA            complement(6599..6688)
                     /locus_tag="ACG8M5_002449"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6652..6654),aa:Ser,seq:gga)
     gene            complement(6728..6801)
                     /locus_tag="ACG8M5_002450"
     tRNA            complement(6728..6801)
                     /locus_tag="ACG8M5_002450"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6765..6767),aa:Asn,seq:gtt)
BASE COUNT         2064 a         1501 c         1106 g         2137 t
ORIGIN      
        1 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
       61 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
      121 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
      181 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
      241 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
      301 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
      361 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
      421 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
      481 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
      541 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
      601 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
      661 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
      721 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
      781 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
      841 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
      901 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
      961 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
     1021 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
     1081 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
     1141 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
     1201 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
     1261 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
     1321 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
     1381 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
     1441 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
     1501 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
     1561 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
     1621 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
     1681 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
     1741 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
     1801 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
     1861 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
     1921 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
     1981 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
     2041 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
     2101 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
     2161 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
     2221 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
     2281 ctcttattga tcaaataata aaaagtaacg taatacaaag caaaaaagaa aattccgatt
     2341 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
     2401 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
     2461 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
     2521 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
     2581 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
     2641 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
     2701 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
     2761 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
     2821 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
     2881 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
     2941 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
     3001 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
     3061 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
     3121 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
     3181 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
     3241 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
     3301 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
     3361 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
     3421 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
     3481 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
     3541 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
     3601 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
     3661 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
     3721 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
     3781 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
     3841 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
     3901 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
     3961 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
     4021 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
     4081 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
     4141 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
     4201 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
     4261 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
     4321 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
     4381 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
     4441 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
     4501 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
     4561 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
     4621 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
     4681 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
     4741 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
     4801 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
     4861 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
     4921 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
     4981 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
     5041 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
     5101 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
     5161 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
     5221 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
     5281 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
     5341 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
     5401 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
     5461 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
     5521 cttgccttac cgcttggcta taccccaaga ataaaaggcg aatgatggga atcgaaccca
     5581 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
     5641 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
     5701 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
     5761 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
     5821 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
     5881 aatctataaa ataaagaata aattatagaa actggggtag ctggattcga accaacgcat
     5941 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
     6001 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
     6061 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
     6121 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
     6181 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
     6241 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
     6301 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
     6361 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
     6421 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
     6481 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
     6541 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
     6601 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
     6661 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
     6721 atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt catgcgctac
     6781 taccaactga gctatgccgg aataacat
//
LOCUS       DAZKMZ010000103         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000103 DAZKMZ010000000
VERSION     DAZKMZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.106"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8M5_002451"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8M5_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M5_002452"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M5_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0359658.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8M5_002453"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8M5_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8M5_002454"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8M5_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0359659.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8M5_002455"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8M5_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0359660.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8M5_002456"
     CDS             3582..4271
                     /locus_tag="ACG8M5_002456"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0359661.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8M5_002457"
     CDS             4285..5787
                     /locus_tag="ACG8M5_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359662.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8M5_002458"
     CDS             5800..6276
                     /locus_tag="ACG8M5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0359663.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8M5_002459"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8M5_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKMZ010000104         6217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000104 DAZKMZ010000000
VERSION     DAZKMZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.107"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACG8M5_002460"
     CDS             <1..271
                     /locus_tag="ACG8M5_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359664.1"
                     /translation="VKDIALDLNYTLAKMNSVIQQAESFCERYPEYKLSFLSENKMIK
                     VEFSSQFLLSKVYSILLEGTIGYILLDSLYKGTYQSLENLSQKII"
     gene            403..1431
                     /locus_tag="ACG8M5_002461"
     CDS             403..1431
                     /locus_tag="ACG8M5_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359665.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1457..1897)
                     /locus_tag="ACG8M5_002462"
     CDS             complement(1457..1897)
                     /locus_tag="ACG8M5_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0359666.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2057..2563
                     /locus_tag="ACG8M5_002463"
     CDS             2057..2563
                     /locus_tag="ACG8M5_002463"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0359667.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2731..4644
                     /locus_tag="ACG8M5_002464"
     CDS             2731..4644
                     /locus_tag="ACG8M5_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0359668.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4649..5650
                     /locus_tag="ACG8M5_002465"
     CDS             4649..5650
                     /locus_tag="ACG8M5_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0359669.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5852..6058)
                     /locus_tag="ACG8M5_002466"
     CDS             complement(5852..6058)
                     /locus_tag="ACG8M5_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0359670.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2282 a          945 c         1239 g         1751 t
ORIGIN      
        1 ggtgaaggat atcgctttag atctaaacta caccctggct aagatgaatt cggtgattca
       61 acaagcagaa tcgttttgcg aaaggtatcc agaatataaa ttatcttttc tttcagaaaa
      121 caagatgata aaagtcgagt tttcttctca gtttttactg agtaaagttt attctatact
      181 tttagaggga acgattggtt atattttact ggattcactt tacaaaggta cttatcaatc
      241 cttagaaaat cttagtcaaa aaattatata agtttgcgta cgattcaaag aaaattaaga
      301 gaacttaata ctattttgaa aaactatcat ttatcttgca gcttgaaaaa aggtaatccg
      361 atagaaggag aagaataccg tatccgatac ttttttcact tgatgtattg gcagtcattc
      421 gacgaaaatc attctgatat attatcaagt ataaaaaata aacgtcaact attagaaaaa
      481 tttgcgaaac atgcgccata tacgcgtagt atagatagaa agaaatggat cagcctacta
      541 agaatttcgt tgcaacgaat gaaacaaggc cactcgatta cgtatgtccc agaagagatg
      601 aacaaatttg tgcatccatt gataacaaaa gaagagttta cgcattacat actagagcct
      661 ttttttgaag caaacttctg gtcgatgaat cacatcactc caacagagca agcttacctt
      721 tattttatgt ttggtgtaat gaacacctat ttggaagaag atttgttgga tactccagat
      781 aaagtacaag aaagttgggg ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac
      841 ttcaagattt gttttacaac ttcagaaaaa aattatctat atgtcaattt ggcaatgatc
      901 catttgtatt caagggtttt tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca
      961 gaagacagag attttcagca tatatttcca gtgatgtatc caatcatgca acagtttttt
     1021 agtggggtag ctcgcagaat tccagagctt aatacttatt atgaaaagaa cagacggttg
     1081 atttttcaat actgtatgtt agtcagggat atttttataa aacacgaaca acctgtcata
     1141 ttctatattc aatcaaagta tggtaaaaca caggaattat gggcaaagaa aaggatatta
     1201 tctatgactg aacgcccaat aaaatatagt gcatccgctg atcagttacc tgatattgtc
     1261 attagcgatt atcctatcga caaaaaggca tatgatcaaa cgaatacaca gatattttac
     1321 tggaatggac aacctacaaa aaatgattgg gcgcgtctga aagctgaaat cataaaaatc
     1381 aggaatgaga aaaatagatc catattggaa aaattggatt tcgttaatta agaaaagcag
     1441 attgtttatg ccaaaattaa gacattgcaa aaagattatt ttctttaaaa tcgacaagat
     1501 gaccagaaag caagagttcg ttgagctctt gctttctttt agttttagaa aggaacatcc
     1561 tgattttatc ttcgctaaat ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt
     1621 ttttcaataa agaaggggat gtacgaatct caatatatag ttcactcaaa ctgagttcat
     1681 ctattctgtg aacaagattt ttataactaa tagaatgagt agagagcatt tcggtcacgc
     1741 cgttgtttgt caattttagt aagtggataa attcattttt ggttagtggt tgacgaatta
     1801 agttctgttc gataaacggt atatcatgga cgataagcca acgttttgcg cgtcttgaag
     1861 acgagcagcc agaacctgta tacaatttaa tcatcataca ttcctccaat agctaagtat
     1921 ttatgaaata ataacatgaa gaaatagtcg aaaagtgcca tttagaggtg taaaataaga
     1981 aaatgttgtc acgtataatg gaactagcta gataagaaat actattaaat caagaaaaaa
     2041 gaaagaaggc acaagtatgt caaacacagc cgacgcaata gtagtgatcg atttacaaaa
     2101 aggggtatgt tttgattctt cgccgataca cgaattagaa acagtgacag cattagtgaa
     2161 taaaagaatc aaaacatacg aaacgattgg gaaaccagtt attttcgttc aacacgaaga
     2221 tgctcaatta gtgccacaaa caaaaccatg ggagctattg tcggaattat ctattcctga
     2281 gcacagttat tttgtgagaa aaacccatgc gaattcattt tataagacta atttgagtgt
     2341 tttattaact gaattggact gtcattcgat cgaattatgt ggggcacaag cagagtattg
     2401 tatagatacg actgtgaagt ttgctcatgg attaggatac cagcttatga tgtgtagagg
     2461 agcaacgaca acttatgata atccttttat gaaagcaaag gaaacaatcg ctttttacga
     2521 gaaaatttgg gataaacgat ttctgacatt tttggagcaa taagttgttt cttcgtttaa
     2581 aaaaagcaga atggatactg gtttatccat tctgctttat tacgtaaaat gagaaaataa
     2641 tcctgttcta tttttatggg ataaaccatt tattttttgt ataaaaaatg tttgcgttta
     2701 catataattc tcctagaaac ataaaaagga gtgaatagct tgagaacaaa acaagtatta
     2761 aagggagcgt tggtcatctt atttgctgta agtacaagcg tcccgcaatg gacagtatat
     2821 ggagagagca tttcttccga atcggaatct cgggaggtag aaaccacaga aactactgat
     2881 gctggaaatg aaacgaaatc gagtacttca caaacagaaa caaatactga aacaacggtt
     2941 gagacatctg ttactagcac agatcaagaa acgaccgcta ccacagataa ccaacaaaca
     3001 gaagaagcaa cagctacaaa aatgtcaaac gacgaatcct cttctgctaa agtaagtgac
     3061 aaagagaatg ggacagaaaa tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa
     3121 tatttgaatt acttcggtaa acagatgaca gaaatactta atggaccaac agaagacatc
     3181 aagtcaaaat taatagagtt attagtcact gagcttgctg ttaatgatta tggtgtagat
     3241 aaagagatta tcaaacatct gattgctact tatctattgt acgatatcgc ttttgcaaaa
     3301 aacagtgtgt tgatgtcgat gcttggaggt acagatgatg ggacaacgcc tacagaaaac
     3361 acaggcttct atccaaatac aaaacaactt catcaaactg tactaaacaa acaagatggc
     3421 aaaatgatgg atgtatatgc atattatgtc gatcaaggtt cagataaaac agtcatgatc
     3481 catggcggat ttcgagggaa ttggaataac gggatcgtta cagaagaata caatgatttt
     3541 tataaggccg ggtataattt attatttgtt gattccagag cgactggtaa tagcggaggc
     3601 gattatgtga cctatggaca atatgagtca gatgacgtcc tttactggat aaatcaagaa
     3661 gttcgagagc ggccatctca aaagatttta ctttatggtg gttcgatggg agcagctacg
     3721 atgatgtctg tattagcgaa agatattcct gtcaatgtca aaggaattat tgaaaattgc
     3781 ggatttgcat caattgatga acaactacgt tttacgtaca gccagacagt agcccccgca
     3841 ctaccggatg ccataaaaaa tcagttagac atcattgggg atcaagagca tgaagattta
     3901 tttatgggcc ttttgaaaca atactatttt gatcaggaga tgcatttgga tacaaaagct
     3961 gctctaccta cgattggaat gtcagattca ttgcctaaat tgattatcca tggaacagca
     4021 gatgatgtag tacctgtgtc taacgcgcaa aaattatatg agttatcagg agggtataaa
     4081 gatcttttat tagtagaagg agcagggcat ggaaaggcac aggaagttga ccatgctgcc
     4141 tacacgaaac atgtaacgga ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag
     4201 tatgtggatg aaaacaataa aagtctactc aaagaccagg acgaaatcca gctttacgga
     4261 gcttatggag aaaattatat gacagagcaa aagacattcg aaggatacga attagcaaat
     4321 gttgaaggac cgactgaagg gatttttaat gagacgatac caactattat tttctattac
     4381 aaaaaaatcc cagtagctcc accaaaaaag caagatccga cagagaatgc agacaataag
     4441 ggaaagaatg aacctcgaga ggatcaatca agtgatgata cgcaaccgat tcagatgaca
     4501 gtgaaagtac aaaaaaagac agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag
     4561 aaacattcgc ttctcattta ttcaggtgga tttttatctg ttagcacgat tatcacatgg
     4621 atttggcgaa gaaagagagg gtaaatgaat gaaatggttc aagcgaatag tgattgcagt
     4681 accagttatc atcgtagtta ttttagtcac tgtatttttg attaatcaaa tcacaccaaa
     4741 gccgattagc ttgcttgtta gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc
     4801 ccgaccagat gatttccagc aaaaaatcga acaaatcgat ataaaaaaag aaatatccta
     4861 tccttcaaag tacaaaaata ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa
     4921 attaccgatt cttttctgga tgcatggagg cgcatatgta ggaggggata aaaatgattg
     4981 tcgtgattat ttggaatatc tatgttcaga cacgcagcaa atcatcgtaa atatagatta
     5041 tgaacgatca ccggaagcaa aacatcctac accagttatc caattgaacg aagccattca
     5101 agcagctaaa aaagagataa aagatcaagc agattggtcg aatatttcga ttggtggaaa
     5161 ttcggcaggt gcacaaatta gtggagaata tttgttagca cttcaaaatg aagaaattaa
     5221 aaaagctgat catcttgatc cgactttgga gaacaagcag attacgaagt ttatttcttt
     5281 gtctggtctg ttggatcctt ctgcacttac tcaagtaagt gataaaatct ctagctttct
     5341 ttatgaaaaa tgtggatggg cttactttga taataaggac tttgaacaat cagagaaggt
     5401 caaaagttta gccttggtca gacatgcgga tcgttggacg caaaacacat tcttaacgga
     5461 tggaaataca aatacgttta cgaaacaaat ggaagaagta acttctgtat ttgagaaagc
     5521 gggggtgaaa gtcacaaaag tagattatgc ggagaaaaat gctgttttga accatgaata
     5581 tcagtttgat ttcagcaata aacaagcgca agaaacatat caaaagttag taatgttttt
     5641 caaggagtaa agtcttatga tgcaaaagag gttgtaatct gtcacttagc tccaagtcac
     5701 aaggagcaat ttcaataaac aagcttttca tggtttatga atattgtaac ttgtgactga
     5761 ggagttagag atggcaaggg gcacctttta ttcgaaattt gggaatatca cacgattatt
     5821 gtcacgatct cttttacgta aattttataa gtcagatttg ttgttttgat cgtttaaaaa
     5881 aagcaacaaa tccgaagcct gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa
     5941 agaatgctgg ttccactaat attccgttgt tattgatata aggctgggaa caaagatgaa
     6001 tagcaaataa aatcaatgca ctagcgaaaa aaagcaaaga aatatggata gctttcataa
     6061 gcagcacctc cttttttata attgtatgat aaatagatta agaatctata aatgtgacaa
     6121 gtttgttagg atgtgaataa aatgatcatc tagcgaactt gaaacaaaga tggctaatga
     6181 taagcaatta atgattcagc aatcagatag gtacaaa
//
LOCUS       DAZKMZ010000105         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000105 DAZKMZ010000000
VERSION     DAZKMZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.108"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8M5_002467"
     CDS             725..811
                     /locus_tag="ACG8M5_002467"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0359671.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8M5_002468"
     CDS             complement(932..1621)
                     /locus_tag="ACG8M5_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359672.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8M5_002469"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8M5_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359673.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8M5_002470"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8M5_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0359674.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8M5_002471"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8M5_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0359675.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8M5_002472"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8M5_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0359676.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8M5_002473"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8M5_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0359677.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8M5_002474"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8M5_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359678.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8M5_002475"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8M5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0359679.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8M5_002476"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8M5_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0359680.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZKMZ010000106         6071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000106 DAZKMZ010000000
VERSION     DAZKMZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.109"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1246)
                     /locus_tag="ACG8M5_002477"
     CDS             complement(<1..1246)
                     /locus_tag="ACG8M5_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359681.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIK"
     gene            complement(1257..1925)
                     /locus_tag="ACG8M5_002478"
     CDS             complement(1257..1925)
                     /locus_tag="ACG8M5_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0359682.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2055..2639)
                     /locus_tag="ACG8M5_002479"
     CDS             complement(2055..2639)
                     /locus_tag="ACG8M5_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0359683.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2970..3203)
                     /locus_tag="ACG8M5_002480"
     CDS             complement(2970..3203)
                     /locus_tag="ACG8M5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0359684.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3218..4168)
                     /locus_tag="ACG8M5_002481"
     CDS             complement(3218..4168)
                     /locus_tag="ACG8M5_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0359685.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4165..5007)
                     /locus_tag="ACG8M5_002482"
     CDS             complement(4165..5007)
                     /locus_tag="ACG8M5_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0359686.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5009..5728)
                     /locus_tag="ACG8M5_002483"
     CDS             complement(5009..5728)
                     /locus_tag="ACG8M5_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359687.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         2063 a         1084 c          752 g         2172 t
ORIGIN      
        1 ctttaataag gtcagcaatc ctttccatat taatataatc caacccactt gtaggttcat
       61 caaatagaaa taaattagtt tttgacaatg aagctataga aaccaataat ctttgttttt
      121 ctccaccgga taaatcaaac ggtaattgat ctttcatttt ataaagttcc atttttttta
      181 aaattttttc ttgctgatct ttactaacta tttcattccc cagggctaac tcgctcctca
      241 ctgattctgt gaaaaattgg taatccattt cttgcattac taaaaacggc gatgacaaat
      301 tttttttatt ttcgattatc tttcctttgg tagggcgaat aatcttcatg agtattctta
      361 gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc aataacatct ccaactttga
      421 catcaaaatt tatgtctttt agaatatttt cataagtaat ttcttcaact tttaatacat
      481 ctgcacttac cttttcgata tagggaactt caatcgaact attaaaaatg tcaaatttcc
      541 ttaatgcgtt attgttttca gtttttaaca actgaatagt tgtagttttt acagttctat
      601 ttttaacaaa tagaacttga tcaattaatt ctttcaaata gtaaaaacga tgttcagcta
      661 taacaatagt aaatccttta ttttttaaat cacaaagtaa gtcagcaagt ttcttagtcg
      721 ctttgaagtc caaatttgaa gacggttcat ccaatataat taatttagag tttagcgata
      781 aactacatcc aatagaaatt ttctgccgtt caccacttga tagcgaaaaa atgtttttat
      841 ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta tccatatgct
      901 cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta tttgttgtaa
      961 agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata ctttcttgtg
     1021 ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt ccgtcataaa
     1081 gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc ccagaagaac
     1141 cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca agcgcttgtt
     1201 ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct ttcacctcaa
     1261 atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc taataagctc
     1321 atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt tgttattgca
     1381 ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac taacagaaat
     1441 tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt taaagattct
     1501 ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac tgtaatactt
     1561 aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc attgctagta
     1621 tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa tacaatatat
     1681 aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat aattttcatt
     1741 actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat tttacgaaac
     1801 atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt tccctttcct
     1861 aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg attgattttt
     1921 gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga taaaaatgca
     1981 ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca attctcaatg
     2041 caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa ttgatacaat
     2101 cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat atcatagaat
     2161 gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac taaattttga
     2221 ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt cgtcacaaat
     2281 gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa taaaatcaaa
     2341 tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc
     2401 tctctccaac ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct
     2461 tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct
     2521 tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caggcacagt
     2581 tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc actttctatt
     2641 aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc ctaataagat
     2701 gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt ttattactga
     2761 actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca aaaagcaatc
     2821 ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga
     2881 tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc caatagccat
     2941 tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta atcgtcaata
     3001 aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc atatctaagt
     3061 ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc taaatcatta
     3121 ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata aattagtata
     3181 gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg ccttcagcaa
     3241 ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt cctttttctg
     3301 caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt gatactgttt
     3361 tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt gttgtgcgta
     3421 atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg ttgatttccc
     3481 aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat ccttcacttg
     3541 taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct tctttgtcta
     3601 gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt ttattatcag
     3661 gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg ttttctaaat
     3721 ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag taaataacat
     3781 ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca tttttaacta
     3841 attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa aaaatcaaat
     3901 ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg ggatctttcc
     3961 caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg gtaatatctg
     4021 ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact ttatctttgt
     4081 ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc cccagacttc
     4141 ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta aaaatttttg
     4201 ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa caatacttga
     4261 tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg aacttgctcc
     4321 gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat atgctgtagc
     4381 tgctggtgta attaacattg caacaattaa tatagttccc acactttgca tagctgttac
     4441 tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat tcatccctat
     4501 tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa atagaactac
     4561 tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat cttgtacagc
     4621 taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa ctcctataag
     4681 gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat ctccttttat
     4741 tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa atacaatcgc
     4801 accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca aaacagcatg
     4861 agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac ctactgttcc
     4921 agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt gaaagtcata
     4981 cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat atagtatctc
     5041 cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg tgagcaatta
     5101 gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat
     5161 gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc ataattattc
     5221 gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt
     5281 gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt
     5341 gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt
     5401 ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata gatacacatt
     5461 cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct
     5521 tcaccagttt tttattgata tcttcattat taatggagac ctctccctga tgagggataa
     5581 tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca ataatacctg
     5641 ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac
     5701 aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca
     5761 cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata gtttagattt
     5821 ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta tagtaagtag
     5881 ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta aaactacttt
     5941 attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt tgataagcat
     6001 caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat atgaaaatca
     6061 gtaaactcca t
//
LOCUS       DAZKMZ010000107         5888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000107 DAZKMZ010000000
VERSION     DAZKMZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.110"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..454
                     /locus_tag="ACG8M5_002484"
     CDS             <1..454
                     /locus_tag="ACG8M5_002484"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0359688.1"
                     /translation="VLELQTLKPNDLVVIRLELNGKKVSLSNASAGQKTSAILSMILA
                     YGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVTHNANIPVNGDSEWLISM
                     GDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYGFKQIVE"
     gene            504..893
                     /locus_tag="ACG8M5_002485"
     CDS             504..893
                     /locus_tag="ACG8M5_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0359689.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            880..2379
                     /locus_tag="ACG8M5_002486"
                     /pseudo
     CDS             880..2379
                     /locus_tag="ACG8M5_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3127..4953
                     /gene="ltrA"
                     /locus_tag="ACG8M5_002487"
     CDS             3127..4953
                     /gene="ltrA"
                     /locus_tag="ACG8M5_002487"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0359690.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5066..5888
                     /locus_tag="ACG8M5_002488"
                     /pseudo
     CDS             5066..5888
                     /locus_tag="ACG8M5_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1989 a         1010 c         1285 g         1604 t
ORIGIN      
        1 agttcttgaa ttacaaaccc ttaagccaaa tgatttagta gttatccgac ttgaactaaa
       61 tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa aagacatctg caattctttc
      121 tatgattttg gcgtatggaa attcaccact catattggat cagcctgaag atgatttgga
      181 tagtcaatta attaataact taatcgtaag aagtatcatt acaaaaaagg aaaacagaca
      241 aattttaatt gtaactcata atgcaaatat cccagttaat ggtgattcag aatggttgat
      301 tagcatggga gattcaaaag aaatatcggc tgatcagtgc gggtcggtag atgaacccaa
      361 tatcaagcca agaatatgta ctgtgatgga aggtggcgag gatgctttta caaatagagc
      421 gaaacgctat ggttttaaac aaattgtaga ataacactag agggtgagac atattattat
      481 gtccgccctt tttaggagga aaaatgaaaa aaataaaaag ctataccagt atctgggcag
      541 ttgaaaaagt catttacgca atcaatgatt ttcagttgcc ttttcctgtg acttttaacc
      601 aaatggcttg gttcgtttta tctttactgt ttgtcatcgt atttgcccat gtgcctccac
      661 tatctatgat agaggaggtc tttttaaaat acttaggcat accggtggct gtcacttggt
      721 ttatgtcgca aaagacgttt gatggcaaga aaccactggg atttttacga tcctttatca
      781 gctatcactt gcgatttaag gtgacttttg caggaaagaa agtcaaagaa cagaagaaac
      841 gatgggatga acccagtact cttgtaagga gtgtgaacta tgtatccgat taagtatatt
      901 gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg cctactatga attagtgcct
      961 tacaattact cctttttgtc acccgaacag aaatttcaag tgcatgataa ctttcgccaa
     1021 ctaattgcgc aaaatcgtga aggcaagatc catgcgttac aaattgccac agaaagtagt
     1081 attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg
     1141 aaacaacgaa ttgatataca gacagatgca ttagtatcca tgattggcga tagtcagatt
     1201 gattatcgct tttttattgg ctttaaacta attgcgacag atgaagaagt caatctaaag
     1261 agtctcaaga aatctttctt ttctgggctt caagaatttg tctatggagt gaatcatcat
     1321 ttgatgggcg attttgtttc cttatccaat gaagaaattc gctgctattc taagcttgaa
     1381 aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca ggcgagtcac accgagtgac
     1441 ctgacgtatt tgattgaaca tatttatggc gaaaaaggga taccgtttga agaatacgaa
     1501 tttcagctac caaagaaaaa gctaaaatca gaaacactgg tgaaacgtta tgacttatta
     1561 cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct tacggatgga acatgagaat
     1621 catgagtcgt acgtagcgta tttaacaatt aatacaattg tgggggagat ggaatttcct
     1681 tcctcagaac ttttctatta ccagcaacaa caatttacat tcccaatcga cacaagtatg
     1741 aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa
     1801 ctgaaagatt tagacaatca tgcttaccaa tctgacaatg aaacgaattc caatgtctta
     1861 gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcaaa agaatctatg
     1921 tacaaactga gttatgtggt tcgagtgagt gccgaatcag tggatgaact aaagcgacgc
     1981 tgtgatgaag tgctcgattt ttatgatgat accaatgtaa aactagtccg gccctttggc
     2041 gatatgatgg ggctacatga agaattctta cccttaagta aacgatatat gaacgactat
     2101 attcagtatg ttacgtctga ttttttggcg gggcttggtt ttggggctac tcagatgcta
     2161 ggagaattag aagggattta ctttggctac aatgtggata caggtcgtaa tgtttacctg
     2221 aaaccggcat tggcttcaca aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct
     2281 ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca atctcttagt ctactatgct
     2341 gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag ggggtgcgaa acgttcctac
     2401 ttgaaaagag taggcacagc acttcattaa atcaatattt aatgtgtgtt ggagaactaa
     2461 acacacgaat agtcgaatga cggggtaacg ccctgaaagg ctactttaat actctgactg
     2521 gcagaaactt gagtaaacaa ggctctgtca gaagctcggt gaagtcggca gacagacacc
     2581 gtaacagata atgctgacga aagattccca gaggtgggcg ttgtgtccta tatgccggaa
     2641 gtctataaaa tatctatggt tagaatgtag atgagaggtc tactgacgaa tccttgaatg
     2701 tacaggtcta tatcggggct ggatagaaat gtccagtgac acaaacagtg tcgctactgt
     2761 gggtgagtaa gactccgcat ttatgaaaat ccatataacg ttaaaggcgt tatcaaggta
     2821 gcaggcttat agaggaaacc taaggatata tgtacagata gtgtgtttgg aacgttgaaa
     2881 gctgacaaca tggagatttt actgtctgtg aagtgttcac atcagaaagt tattgtgaga
     2941 ttagcaatga tataaggtgt gttaatgtca gtgagagtgg tggcacagta cctatgaaac
     3001 cgtgataaca agcggtggag ggatagccac tagtcatatg aagaatagaa ttactaagaa
     3061 aaatggtatg ggttcgagta agactaagag atgtacttgc tcctgcaaac aaggagggta
     3121 cagaccatgc taaagaaaaa caagctccgc tataacgagt attatgatat gcaacatatt
     3181 tatgacgagt tatacgcaca gagcaagaac ggtaataact tttataagtt acttgaaatt
     3241 attggttcgg aacaaaatat ttgtttagct taccgtaatc tgaaatcaaa cagtggcagt
     3301 aaaacggctg gaacggatgg aatgacgata gacgatataa aacaactcag caatgctgag
     3361 attgttgcta ccgttcggga aagccttagc aactaccgcc ccaaatcagt cagacgtgtt
     3421 ttcattccaa aagctgggag cgataaaatg cgtccattgg ggattccgtg tatctgggac
     3481 aggctggtac aacaatgtat cctgcaagtc ctagagccga tatgcgaacc aaagttccat
     3541 aatcattcct atggattccg tgcgaatcga agtgcccacc atgcagtcag cagagtaacg
     3601 acactgataa atttatcaaa atatcattac tgtgttgacg tagacattaa aggatttttc
     3661 gataatgtca atcatggaaa actattaaaa caaatctgga cacttggtat tcgggataaa
     3721 agattgattt gcattatcag caaaatgctg aaagccgaaa ttgacggcga gggtgtccca
     3781 gaaaaaggaa cgccacaagg cggtctgtta agtccactgc tctccctcat agtccttaat
     3841 gaattagact ggtgggtaag cagtcagtgg gaaacattcc agccgaagaa ccgcagtaaa
     3901 aatgggtggc ttcaatacgc aaagaaatat accaaactga aaagcggttt tattgtacgt
     3961 tacgctgatg attttaaaat catgtgttca acctatggag aagcacaaag attttaccac
     4021 agtaccgtag attttcttaa taaaaggctg aaattagaaa tcagtccaga gaaatcaaag
     4081 gtagtaaatc tgaagaaaaa ttcatcagat tttctggggt tcaaaatcaa agtcattcca
     4141 aaaggaagaa cgaaacacgg ttatgttgcc aaaacagata tgaaccaaaa agcattaaag
     4201 aaagcgaaaa caaatctaaa gttaaaggta aaagatatag ccagacatac gactggcttc
     4261 aatatcagca gatataatct gacagttatc ggtatgcaga attactactg catagcaact
     4321 aacgtatata acaacctgac agaagtaagt tacgctctct taccgaccat acgaatccgt
     4381 ttaaggaaca ttgcaaaatc agttccattt gaaagtacaa gttcagaatt tcagagcagg
     4441 acaaaaggaa tcagaccaaa aacaaaaata gtgatgattg cggataatcc gctattgccg
     4501 attcaaggag tacaacacaa aaatcctatg aatttctcgc aagatatttg taattttaca
     4561 aagcaaggga gaaacaaggt gcatgaggac gttgtagttg taacaaaaga agaaatccga
     4621 gcgttgttgg aaaatgaaaa ccctgccgat agcgtagagt ttaacgacaa tcgtatttcg
     4681 gcatatatcg cacaacaagg caactgctat gtaatgaatc gtcgaggaac tccgtccact
     4741 ctgatatgta ttcacaaatc agacaaagga gataaccttg acagatattc taatctggca
     4801 tttgtggaaa ttcccattgc aaaggcaatc ctaacagaat cagcagacga agcaaaatca
     4861 ctgttaaaag gttatgttct taatagtcag cagaagaaaa aactgaacag aatacggacg
     4921 aattacggat accagacaat aactgggaaa taacttataa atctatttgg atatgatgga
     4981 acgccgtatg aagcgaaagt ttcatgtacg gtgtgaagcg ggggaaaact tggagataac
     5041 atcaaaaagt tacctatcgc tatagcgagg agggtggaaa gaaaccttgc cagaaattgc
     5101 ggatgaaatc agtatcctca acttgacgag taagtcagaa aatcaaggtc tgcttgatcc
     5161 ttatgtgatt atgaagaaaa agaaagactc ggaaagttta gcaattgata ttctgacttt
     5221 tttaacagga atttcaagtc gagacgggga gaagtttcct gtgttacgta aagccattcg
     5281 aacggtgact caaagtgatc aacgggggtt attacaagtt attaacgaat tgcgaaaaga
     5341 cgggtcacca atcgcggaaa atatcgccga tcatatcgag tcctttaccg attatgattt
     5401 tgctcatttg cttttctcag acggctcgat tacccaatcg attagtttag ataaacagct
     5461 aaatatcatt caagtggcgg atttggtttt accggatgca gaaacgactt ttgaagaata
     5521 taccaccatg gaattgctta gtgtttcgat gctaatcgtc atttccactt ttgccttgga
     5581 ctttatccat agtaaccgag aaatcttcaa aattgtcgat ttggatgagg cttggagttt
     5641 tcttcaagtg gctcaaggaa aaacgttatc gaataagctt gttcgtgctg gtcgggccat
     5701 gaatgctggg gtctatttcg tcactcaaaa tagcgacgat ttattagatg aacgtttaaa
     5761 aaataatatc gggttgaagt ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac
     5821 cttggaattc tttggtttgg atcaggagga tcaaagcaat caaaaacgcc tgcgagattt
     5881 ggaaaacg
//
LOCUS       DAZKMZ010000108         5865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000108 DAZKMZ010000000
VERSION     DAZKMZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.111"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..2134
                     /locus_tag="ACG8M5_002489"
     CDS             2..2134
                     /locus_tag="ACG8M5_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0359691.1"
                     /translation="MQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAF
                     GLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWI
                     ADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFH
                     IVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSY
                     LLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSI
                     FLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVN
                     SSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLIS
                     SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNE
                     IFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIV
                     GVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTI
                     IENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLAL
                     ARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILV
                     LHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2103..3518
                     /locus_tag="ACG8M5_002490"
     CDS             2103..3518
                     /locus_tag="ACG8M5_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0359692.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3551..4585
                     /gene="sppA"
                     /locus_tag="ACG8M5_002491"
     CDS             3551..4585
                     /gene="sppA"
                     /locus_tag="ACG8M5_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0359693.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4587..5108
                     /locus_tag="ACG8M5_002492"
     CDS             4587..5108
                     /locus_tag="ACG8M5_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0359694.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5105..5491
                     /locus_tag="ACG8M5_002493"
     CDS             5105..5491
                     /locus_tag="ACG8M5_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0359695.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2094 a          800 c         1101 g         1870 t
ORIGIN      
        1 tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga ttttaaaata
       61 ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg atttagaggg
      121 tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt gtatggctat
      181 tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa ttgcacatat
      241 attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg gaaacgtact
      301 atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt ttcaaaaaga
      361 atggacaggt attcttttat tacctatgcc tagagaaact tatattccta ttaaagaaaa
      421 agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat tagtattcca
      481 catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat attattttca
      541 aggtattctt gattttttca tccctaatca agcacgttca actttaaata taatttccat
      601 tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta gttatcttct
      661 tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca aacatgtgtt
      721 aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt ctcgttttct
      781 tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat ttttagatat
      841 tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt tgtttttaat
      901 cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga aaagctatga
      961 aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta tcatagaaag
     1021 tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat ataaccgtat
     1081 agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc ttgataatat
     1141 tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt tatggctagg
     1201 ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat acaatgcttt
     1261 actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga aaatgcaaac
     1321 ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag aaaaatcgaa
     1381 ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca ctgtaaaaaa
     1441 cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat gtacaattcc
     1501 ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa ctcttgcaaa
     1561 gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa acattaatca
     1621 cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc aagagtcatt
     1681 tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc caccaagttt
     1741 cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta atcaacagcc
     1801 attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag ggcaacgaca
     1861 acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt tagatgaagc
     1921 aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc tcaatttaaa
     1981 agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat gtgatcaaat
     2041 tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac ttcgttttgc
     2101 cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt atgcagaata
     2161 ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc taccgttggg
     2221 tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc tttgctaaaa
     2281 aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag aagttacaag
     2341 tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt gttcataagg
     2401 acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct cagatagaac
     2461 aagaaaacca acgtattact caacaaataa agatggctca gctatttatt gaaagtataa
     2521 gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat caattaaaga
     2581 gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa ttaaatgatc
     2641 aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa attgagagtt
     2701 cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat aatcaatcat
     2761 taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa caactaaata
     2821 atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc ataaatgaac
     2881 aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct aaattagtta
     2941 aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag caagagctag
     3001 aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa caagagaaga
     3061 accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta aaagctcctg
     3121 tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta gccaaaggaa
     3181 cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt acagcacttt
     3241 tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt aaattggatc
     3301 aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca gaaaattcta
     3361 ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca actcaatcgt
     3421 ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa aaaacatatt
     3481 ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta agaatatagg
     3541 aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg gattattgtt
     3601 actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat caaatacaac
     3661 cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag aaacgttaga
     3721 agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta ttgcagatac
     3781 aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt taacacagct
     3841 aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa attcacctgg
     3901 gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta aaaagctaga
     3961 tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact acatttctgc
     4021 tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg gtgtcattat
     4081 ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg cgacgtataa
     4141 aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg aagagaataa
     4201 agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg tagcaaaagg
     4261 aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt atgatggtaa
     4321 ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg cattagattc
     4381 cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa atgacacaac
     4441 tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc taaaggcaac
     4501 tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata ctccaaaacc
     4561 aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa actttgccta
     4621 attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat ttaatttgta
     4681 ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga ctggaatctg
     4741 acaaatccat tattactttt tatggtatag cgtcattagc tatttattta acctattttg
     4801 tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt gggataagag
     4861 tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt gagtgtatat
     4921 gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct gctttcacgc
     4981 ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt attcgttcat
     5041 tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat ctggagagaa
     5101 ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa taacagggat
     5161 tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg attcaactac
     5221 tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc taagcgggaa
     5281 tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca taataggttt
     5341 tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca ttttatctat
     5401 tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag gtattattgc
     5461 gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa cgtgaaacga
     5521 ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg atggaagaca
     5581 tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa aaagacactc
     5641 tctgattgtt gtattaatgg aaaaggggaa agcagcaagt aaatttagtt tttctattaa
     5701 atattcttca ctttttaacc ttttatagta cttagattta atcctatatg tttagagagt
     5761 tcattggcag catgattttg caaagcttct cgaattattg ttatattaga aactttactg
     5821 ttcataatta tgcttctttt ttaactcaaa gtccaaaggg ctaca
//
LOCUS       DAZKMZ010000109         5700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000109 DAZKMZ010000000
VERSION     DAZKMZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.112"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..169
                     /locus_tag="ACG8M5_002494"
     CDS             <1..169
                     /locus_tag="ACG8M5_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HFQ0359696.1"
                     /translation="GAQQTLIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELS
                     LPVKLIKKNTA"
     gene            291..1709
                     /locus_tag="ACG8M5_002495"
     CDS             291..1709
                     /locus_tag="ACG8M5_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0359697.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1780..3249
                     /locus_tag="ACG8M5_002496"
     CDS             1780..3249
                     /locus_tag="ACG8M5_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0359698.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3259..3726
                     /locus_tag="ACG8M5_002497"
     CDS             3259..3726
                     /locus_tag="ACG8M5_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0359699.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3762..4634
                     /gene="scrK"
                     /locus_tag="ACG8M5_002498"
     CDS             3762..4634
                     /gene="scrK"
                     /locus_tag="ACG8M5_002498"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0359700.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4716..>5700
                     /locus_tag="ACG8M5_002499"
     CDS             4716..>5700
                     /locus_tag="ACG8M5_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0359701.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKL"
BASE COUNT         1934 a          815 c         1152 g         1799 t
ORIGIN      
        1 tggagctcaa caaacattga tttataatcc agaattatca actattcaac agcctataga
       61 tcaaatatct aaagtagcag taaaaaaatt attgaatatg attaagggcg aaaaggagac
      121 ggatgaactt tcacttccag taaagctcat aaaaaaaaat acagcataag ttaaatcggc
      181 gaaaaattcc ttcgtcgatt taaaaatatt tttatgaaac gggttgacat atgaaacggg
      241 ttgacatata ttagttgtga agataaaaac cttttcactg gaggaagaaa atggagaaat
      301 tattaattaa aagcaataat catcaacttt actaccaacc taaggatgtt tgggtcggag
      361 atgttatgcc ttacgggaaa gaaggaaaat tctttattta tcatcaacgt gatactagaa
      421 atccagttcc ttttggagaa ccgtttggct gggctttagc aactacgaaa gattttgttc
      481 attttgaaga ctttggggaa tctcttttaa gaggggatga caatgagaga gaccagttta
      541 tctacgctgg ttctgtattt gaggcaaatg gaaaggttca cgcattttat acagggtata
      601 atcgtgaatt cttagcgcag ggaaagacat ctcaagtgtt gcttcatgct gagagtgatg
      661 attttgttca ttgggaaaaa tcagaaacag cattggagtt agaaccacaa gaagggtatg
      721 atattcgaaa ctggagagat ccatttgtcg tttgggatga tgaaaaagaa gaatatttgc
      781 taattttagg ggcgcgaaag ggtcaggata aacatctaca aaccggacgt ttagtggaat
      841 ttacatcaaa agatttaact aattgggaat tcaaaggtga tttttgggct ccgggattgt
      901 ataccatgtt tgaaatgcca gaacttttca agattggtga ctggtggtat cttgtctatt
      961 cagaatactc agatggaaat aaaatactct atcgaatgag tcaaagtttg gaaggtcctt
     1021 ggattaagcc taaagatgaa gcttttgatg gacgggctta ttatgcaggt agaacagctt
     1081 atgatggaaa acgtcgagtc ctatttggat gggtaccaac aaaagatcaa gaaaatgata
     1141 aaaacaatta tttatgggga ggtactttgg ttcctcatga agtctatcaa aaagaggatt
     1201 tttcacttgg ggtaaaaccc atagacgaag tgtggaatgc attcataaat ttacatgata
     1261 ttgccgatgt agagatagaa acagcagaca caagagaaga agtaacgctt gtcaaaaata
     1321 gtggaacttt gtttaattta gaaacaacaa tttcatttga tgagaatctt agtgactttt
     1381 cactgagatt atataaaaac cttgagacag atgaatctta cgaatatcgc ttcaatttag
     1441 atgaggggcg tcttacattt gataaaaacc cttgttaccc ttggtttcaa tatatggata
     1501 aaggacttat tagacctata tctttaaagc ctaaccaatc ttataaacta aaagtgattg
     1561 ttgatgacaa tataatgaca ctttatattg atggaatcgc tcttaacgct cgtgtacatg
     1621 gttgttttgg tcaagcaatt actattacag caacaaatgg atcaatgact gcaataaata
     1681 ccaagttttc aaatgaaata aagaaataat aaacgtcaat catgtggcag gtctgaaaat
     1741 caaacatgac ctgtcacatt taaaagggag gataaatgaa tggactacaa aaaaattgcc
     1801 gaagatgtat tggaaaacgt tggaggaaaa gagaatatct caagcgctac acactgcatt
     1861 actcgtttac gtttaattct taaagacaaa agcaaagcga atatggaaat gcttgaggaa
     1921 atagaaggtg caaaaggtgt tcttttcaat agtggacagt tacaagtaat tttcggaaca
     1981 ggtataattg aaaaggttta caaagagttc gttgaattga caggagctaa ggaagtatcc
     2041 gtttctgaaa tgaaagacga aggagtcaat aattcaggaa atagacttca acgagctttt
     2101 aaagttttct cggatatctt tattccgatc attccagctt ttattggggc agcaatggtt
     2161 ttaggaattc gttcgttgat taccactcct ggaatgtttg gaatggatgg ctcattcgct
     2221 gatcacaatg tattcctaaa tgatttatcc aaatttctgg gtattattgc aaccacattt
     2281 gattatttac cagttttagt tatgtattct gcaacaaaac gttttggcgg aaaccctatt
     2341 ttaggattac ttgtaggatt tgttatgatt catccaggac tagccaatcg gaatgatttc
     2401 gtcatgggaa ctataaagcc tgattattgg catttatttg gattaagcat tccagcagtt
     2461 gcgtttcaag ggggtgtttt tccagcaatt ttgacttctt ggtttttagc aaaagttgag
     2521 aaaattacaa ataaatattc tcctcaagta cttgcttaca ttttagtccc aactgtgaca
     2581 attgttcttg ccaactttgc actattttta gtgtttggtc cattggggaa ttttatcggt
     2641 aatgttttag gtggaatcat tgatttccta tatatgaaga tgggtgcctt tggtgctttt
     2701 gtttttgcag gcgcgttaca accattagtt gtaacaggaa cacaacatgc tattcaaggt
     2761 attgaggcaa acttagtcgc aacaactgga ttcaattata tccaacctat ttggtcagtt
     2821 tcaattattg cccaaggtgg cggttgtatt ggtatgtatt tactctttaa gaaaaaatct
     2881 aaagatagag atattgcgat ttcaagcttt attccaacac tggtaggtat tactgaaccg
     2941 gctatctttg ctgttaattt gaaatattca atcgttcctt ttgtttgctc gtttattggt
     3001 gctggttttg gtggagcatt tatgaaaatt tttgatgtaa aagccattgg acaaggattg
     3061 actgtattgc caggtctgac tattgctaat ccagttcttc cttatatatt tggtaatctc
     3121 atagcattca ttttaccaat catttttatt ctcgcttatg ataaatataa agtgtttatc
     3181 cctgaagcta aacaccgtaa atttgcagaa aaaactgact caaaaaaatt gccagttcaa
     3241 gtaaagtgag atgagagcat gttaaatttt tttagaaaaa atgaaataag cgttgtagca
     3301 cctgcaaccg gtgtttttga aaaactggag aaattaagtg atccagtctt ttcaaaagga
     3361 atgatgggac aaggatttgc aattgatgca aaaaacgaaa cgattgtatc tccaatcgat
     3421 ggttttgttt caagtatttt tcctacaaaa catgccttag gaataaaaac aaaatcagga
     3481 attgagatac ttgttcatgt aggaatagat acagttgagc taaatggtga gggatttgat
     3541 attaaagttc aagaaggaca aaaagttaag gctggggata ttcttatgac cattaatttt
     3601 gtagtttttg aaaagaatca caagactaaa gatgtcattg ttgtttttcc agactaccaa
     3661 gacgaaattt tcattccgaa acttgaagaa gtaaaacaag gacaagaatt gtttgttctt
     3721 ggctaatgat aagtaattaa taatagaatc gaggaaaatg tatggttttt ggggctattg
     3781 aagctggtgg aacaaaattt gtttgtgcca ttggagataa agatttgaac atatttgaaa
     3841 aagtcagctt tcccacgact acacccaaag aaacaatgaa tttagtttta gattttttta
     3901 atcatcataa agatgattta gaaggtatcg gagtgggatc gttcggtcct attgatattc
     3961 attgtgaatc taaaacgtat ggcttcatta catcaacgcc aaaacttgct tggcaggatt
     4021 ttgattttat tggaagttta aaaaagcaac ttaatattcc aactgcttgg acgactgatg
     4081 taaatgcctc ttgctatggt gaatatgttt ctggaaatgg cagaggcctt tcaagtgttg
     4141 tttactatac cgtaggaacg ggcataggag gtggcgctat ccaaaacgga aacttcattg
     4201 aaggttttag tcatccagaa atgggacata tgcttgtaaa acgacacaat aaagacaact
     4261 tttctgggac atgtccattc cacggagact gtttagaggg cttagctgct ggtccggcaa
     4321 tcgaaagaag atcagggaaa aagggacaag aagtgactga aaatgacgtt acttgggaaa
     4381 tagaagctta ttacattgct cagtgtgcat ataatacaac attgatgtta tcacctgatg
     4441 tcattatatt tggtggcgga gtaatgaaac aaaaacactt aaaaaagaaa gtacaagata
     4501 agtttattga gttgatcaac ggatatgtcg ctattcccga tgttgacaat tatattatga
     4561 caccaaaatt gttagataat tctggtattg taggttgttt agctttagca agagacgtta
     4621 aagaaaaatc ttaatttgga aaaaagatcg tctcgactat ttgtttggtt gatgcgatct
     4681 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     4741 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     4801 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     4861 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     4921 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     4981 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     5041 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     5101 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     5161 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     5221 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     5281 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     5341 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     5401 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     5461 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     5521 aagatgaagc aattacaatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     5581 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     5641 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
//
LOCUS       DAZKMZ010000110         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000110 DAZKMZ010000000
VERSION     DAZKMZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.113"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8M5_002500"
     CDS             complement(488..1297)
                     /locus_tag="ACG8M5_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0359702.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8M5_002501"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8M5_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0359703.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8M5_002502"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8M5_002502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0359704.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8M5_002503"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8M5_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359705.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8M5_002504"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8M5_002504"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0359706.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8M5_002505"
     CDS             4179..4913
                     /locus_tag="ACG8M5_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359707.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8M5_002506"
     CDS             4897..5607
                     /locus_tag="ACG8M5_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359708.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKMZ010000111         5646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000111 DAZKMZ010000000
VERSION     DAZKMZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.114"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG8M5_002507"
     CDS             complement(<1..1128)
                     /locus_tag="ACG8M5_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0359709.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG8M5_002508"
     CDS             complement(1164..1874)
                     /locus_tag="ACG8M5_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0359710.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2802)
                     /locus_tag="ACG8M5_002509"
     CDS             complement(1942..2802)
                     /locus_tag="ACG8M5_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0359711.1"
                     /translation="MFVAIWGKWSTRRIKMKVLGLGDNVVDKYENLGIMYPGGNSLNF
                     AVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGD
                     RIFLGSNSGGVTREYPIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYD
                     FSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIG
                     YDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVD
                     GSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG8M5_002510"
     CDS             complement(2768..3736)
                     /locus_tag="ACG8M5_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0359712.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5646
                     /locus_tag="ACG8M5_002511"
     CDS             4499..>5646
                     /locus_tag="ACG8M5_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0359713.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA"
BASE COUNT         1735 a         1158 c          901 g         1852 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgc
//
LOCUS       DAZKMZ010000112         5538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000112 DAZKMZ010000000
VERSION     DAZKMZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.115"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG8M5_002512"
     CDS             complement(<1..302)
                     /locus_tag="ACG8M5_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0359714.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACG8M5_002513"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACG8M5_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACG8M5_002514"
     CDS             complement(1016..1306)
                     /locus_tag="ACG8M5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359715.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACG8M5_002515"
     CDS             1708..1944
                     /locus_tag="ACG8M5_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359716.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACG8M5_002516"
     CDS             1951..3903
                     /locus_tag="ACG8M5_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0359717.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG8M5_002517"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG8M5_002517"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0359718.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="ACG8M5_002518"
     CDS             complement(4799..5491)
                     /locus_tag="ACG8M5_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0359719.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1801 a          965 c          899 g         1873 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtga
//
LOCUS       DAZKMZ010000113         5467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000113 DAZKMZ010000000
VERSION     DAZKMZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.116"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..2403
                     /locus_tag="ACG8M5_002519"
     CDS             76..2403
                     /locus_tag="ACG8M5_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0359720.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2400..3314
                     /locus_tag="ACG8M5_002520"
                     /pseudo
     CDS             2400..3314
                     /locus_tag="ACG8M5_002520"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3387..3902
                     /locus_tag="ACG8M5_002521"
     CDS             3387..3902
                     /locus_tag="ACG8M5_002521"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0359721.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3899..4345
                     /locus_tag="ACG8M5_002522"
     CDS             3899..4345
                     /locus_tag="ACG8M5_002522"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0359722.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4495..5310
                     /locus_tag="ACG8M5_002523"
     CDS             4495..5310
                     /locus_tag="ACG8M5_002523"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0359723.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1940 a         1043 c         1083 g         1401 t
ORIGIN      
        1 atcaataaaa taataaaaat taaatttaga ataacgtgta aaggattatt tgatgatttt
       61 ttattgagag gagggatgaa aatgattaag aaaactcaaa ataaaatcca acgtatatgt
      121 caaaaacatt ttgtttctag actcaaagct gtatctaaaa gaaaatactt acatcccatt
      181 atgatttcga tcatcttgat ttttactttc ttagggagct tatcaagtat ttatgtaaac
      241 gtttatacag aaacgtcaaa ttttgctaac caaacgacag aggagacaaa tagtccaccc
      301 tattctgaaa tacagtccat agaaagcacc aatcagtcta gcaacaacga gacaacctca
      361 accacatcct cgactcaact gattgatccg acagaaaaag aaatcacagc agcaaaagca
      421 gcaggggaga aagcttattc agaaacaggt agaagccaga catttagtac tgttgcggcc
      481 actgcgaatt tcaacggaca taccatacca gatggatata cagtgatata tgccaaagat
      541 ggagtcattg ttgcggttaa cggatcgatg agtgcggata aaactgcgtt acaaggcgta
      601 accaaagcaa tcgcttttat tgataaccgt ccagatatcc ataatctgtt tattggctcc
      661 acagtaaatg gtactactat cacattccca aaaagtatct atatgggggt ggctaccgct
      721 ggaaatacga tcgattcagt tacacccaat gcggataaaa gttattcttt tacaccctca
      781 acatggggag ggcttcctgc cggaggaggt atgagtactt tttcaatcgg tatacagtta
      841 gcaaatgata ttccattcac ggggtactcg atcaaaaatg ggcagatcgt tgatggtaag
      901 ggaaatgttt caaattttcc aacctatcaa gcagcaacgg ttcaggtcaa tgcgaatcgg
      961 acaggaactt tttcttatac agtcaatggt ggagcagcaa aaatcggaac gtacgggact
     1021 acattatctg gaatccatgt gggtgacaaa gtggttatca ctccaacagc agtaacgggc
     1081 tattcttttg cacaaacaaa cggttctttc acagccctag aaagtaacgt cgatacggtg
     1141 acctatacag gcaaacaaac accaatcact attaagtatc aagactattt tggacagatg
     1201 attgctcctt ctaaaataat gaatcttact tacggaagtg ctgcacaaga cctgacgact
     1261 aatacgccta ctatcaacgg atataccttt acagctgttt cagcgataga aacaaagaac
     1321 caatcagcaa tgagtgtttt agctagttta gatacaaatg gaaatgtaat cgtaaaggac
     1381 gcaaatggaa aacagatttc tgaagtcatc ctatattata agacaaatgc aacagtatca
     1441 atcaatgcaa atggcagtaa gtactatgat ggtctttctg ttgtaccgat cgttaaatat
     1501 gcctttaatg ataaaacaga atcaacatcc cttttaaaca atgatctcag tattgatcaa
     1561 atcacatgga atccaactga tttcaaagcg atgaatgaaa agggagttga agtaactgcg
     1621 cctactgaaa ttggaaccta tacgtcttgg caattgacaa aaagcggact agctaaacta
     1681 gctgccagaa caaactattt atttactatc gtgcagacat ctgatgtcta tacgattaag
     1741 caaatcagcg gtgcggtaga gctcagtggc agtaagacat atgatggaaa agcaggtgtc
     1801 ccgtcaatcc atgtcaagct agcagagggt gtcactagtt cgcttactcc agtacaagtg
     1861 gcacttagca gtatggatta cacagcagat gcacaatcag caaaaaatat ggttaatgct
     1921 ggcagctata caattaaact aacgaatagc ggaatagaca aagtcaaagc agcaaatccc
     1981 aagttgtcat ttactgatat agccaacaca tcaggaactt ataccatcta taaagctaac
     2041 gcagtgatta ctgtcgatga cgctgccttt aattatgatg cccaaagtca ctcgatttcg
     2101 gttggaaatc tccacgttac cggtgttgtc ttaggcgaat cattggatta taccctaaca
     2161 gttaacagcc ggacagatat tggatcacaa atagtaacga ttagtttagg cgaaggaagt
     2221 gtaaacaata attatacgat tatcgcaaca gataccgcta agttgacgat caatccgact
     2281 ctcttgcttc cttcgacagg tgggataggt ctactgccct ttgtcttttt aggactgatc
     2341 tttatttttt ctggcttctt ttattttatc catcgtaaga aaaaagcagg tgaacagcga
     2401 tgaaaaaaac aaaaaatgta cgtgtggata tatttgctgt tctctttttt atcctactgg
     2461 tcttttttat aggttctccg actgtgtcag ctagagaatc acctattaca tttactttgc
     2521 ataatcgaat gttcgaaagc gataagatgc caagtatcag tcaaaacgac ggaataaata
     2581 tcccggataa ctttcaagga gtaaaagggt tgaacggcgt tactttcgct atatatgatg
     2641 tctcggatga attttacaag ctgcggtcag aaggaagtag tgtggaagac gcacaaagaa
     2701 aactagcaca aaaaagtgat agtgaaaaaa tactggcaga aaatgtgaca aaaacagtag
     2761 acgaggaaga gggcattgct tcttttagtg catcggacaa agatgagcaa ggtcgtgacg
     2821 ctgtttaccg atttacggaa ataaagactt cagattaagt aaaagaaaaa tcagcaccat
     2881 ttgtcgttgt gcttcctgtg acagattcat ctaatcataa attgacgacc atccacttgt
     2941 atccaaagag cgaacaaaaa atacctgctg cactcacatt aacaaaaaca gtagagaata
     3001 agcagacaga ttttgcagat ggggataaag tcccttatct catcaccgta acaattcctg
     3061 agaacatcaa cgagatagga acctatacga tcaaagatac agctgatcct cagttatggc
     3121 tagaaccaga aacgatcgat tttttgattg gtggagacaa gattcataca tttcacacac
     3181 aaaaaataaa acatggtttt gtcttatcag attctggaaa agatcttagt tcgtttgctg
     3241 gaaaaaaatt aacgatccgt taccaaatga cattgaaaga aaacagtgaa aaaacgacat
     3301 ttgataacga tgtacgtttg acgactgaca atcaaacagt agaaacacat cttgccatcc
     3361 aaacaggagg caaacaattt gtcaaagtgg atcggcaaac gaaacaaaaa ctagcagatg
     3421 ctcaatttct agtcaaaaaa aatgaccaat acttagcgca agaaaacggg ataaatcatt
     3481 gggtagccaa agatgattca agagcaacga tttttacttc cagtaaagat ggaacatttt
     3541 ctgtccatgg attgtctttt ggaagttacg aactagtgga gattaaaccg cctaaaggat
     3601 atatattgag tcaatcaacc attctctttg aagttgaaaa aggcagttat gatccgcttc
     3661 atcctatacc attggaaatc atcaacgaac caaaagcaaa tccgacgaat aatggaaaaa
     3721 cgccagttca gatgcagaca aatggtccat caaatgaaaa aaatggagga agctttccaa
     3781 aaacaagcga agagatgctt ggaggtttct caattttagg gttattgcta gtcctttcaa
     3841 cagggacagc ttggttttat aaaaaacaac aaaaaggaaa caataggagg gaaataaaat
     3901 gaaaaacatc aaaacaaaaa tatggacgtt tttaggaact gctatcatgc tgcttccttt
     3961 cgttctcggc ctaggaactg cagaggtttc tgcagccgta tcacctacac cagaaaatgt
     4021 tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa taaacaacgg
     4081 aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca acgtatatga
     4141 catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg ctacaccctt
     4201 cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag gtaaatctga
     4261 tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg aaacgtcgcc
     4321 aaatatcgtg ttaagcttac cctagaccct gacaaacctt tagacacgat tcacctatat
     4381 gctaaaaatg tcatcaaaga aagcaatatc aaaatccaga aagctgggaa cttttcaggg
     4441 gtagataagt tagcaggggc gcaattccag atcaaaaatt cagagaatca attcgtgacc
     4501 ggttttgatg aaaacactgg tgtcgctctc tatgatcctg ctgagtcagc gttgacattc
     4561 acaactgata ctgaaggatc catcagtgtc aatggattaa tagacggcaa ttatacttta
     4621 gttgaaacaa aagcaccaga tggttatcaa ttaaccaatc cacaaaaaga aactactttt
     4681 atagtaaaaa acggaaaatt gcaacaaaca accggtatca tccatggcgc agatgaagcc
     4741 aatcttggga atccaaacgg tgtagtcaat cgggaaaata caactacgga taacacgatt
     4801 actgtagaaa atgtccaaaa ctacggtgat ttccacttca tcaaacaaga tgtgaacacg
     4861 caagaaaaat tagccgatgc agaattttct attctaagag caaaagatcc aactgttaaa
     4921 ttaatgaaaa aaatagcaaa ccttgcctca ggagaatacc catatgcttg gtcagatgaa
     4981 gtcacaggtt ctgactggga agaagtgaaa ttgatttctg attcagaagg gaaattttca
     5041 atcgaagggc tgaaacaagg ggcatactgc cttcaagaaa caaaagcacc agaaggctac
     5101 gcattaccta agaatgaagc tgcgtacact gaatttacag tagatgatga tccaacaact
     5161 tctgatgaaa caacgatcga taaccaacct aaaggaatct tgccttcaac tggtggtatg
     5221 gggatcatcg tattcatctt ggtaggtact gctttagttg gaggagcagt gatctatttc
     5281 aaaaaacgcc atcaagaaac agaagcttaa actattggaa acaaaaatca tacgataaat
     5341 aaacgatagt gattcaaatg gggagaaagg ttgtgacaag tggtcggtct cttggtattc
     5401 gctcaacaaa cggcaaaata ggaggaacta ttttgccgtt tgcttcgcta atactcaggt
     5461 caaggcg
//
LOCUS       DAZKMZ010000114         5386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000114 DAZKMZ010000000
VERSION     DAZKMZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.117"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..953
                     /locus_tag="ACG8M5_002524"
     CDS             96..953
                     /locus_tag="ACG8M5_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0359724.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1068..1988
                     /gene="pstC"
                     /locus_tag="ACG8M5_002525"
     CDS             1068..1988
                     /gene="pstC"
                     /locus_tag="ACG8M5_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0359725.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1988..2872
                     /gene="pstA"
                     /locus_tag="ACG8M5_002526"
     CDS             1988..2872
                     /gene="pstA"
                     /locus_tag="ACG8M5_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0359726.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2887..3693
                     /gene="pstB"
                     /locus_tag="ACG8M5_002527"
     CDS             2887..3693
                     /gene="pstB"
                     /locus_tag="ACG8M5_002527"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0359727.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3709..4467
                     /gene="pstB"
                     /locus_tag="ACG8M5_002528"
     CDS             3709..4467
                     /gene="pstB"
                     /locus_tag="ACG8M5_002528"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0359728.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4480..5157
                     /gene="phoU"
                     /locus_tag="ACG8M5_002529"
     CDS             4480..5157
                     /gene="phoU"
                     /locus_tag="ACG8M5_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0359729.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         1698 a          954 c         1199 g         1535 t
ORIGIN      
        1 gagaatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
       61 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
      121 caataatagg attagccatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
      181 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
      241 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
      301 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
      361 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
      421 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
      481 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
      541 tgaaccgcgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
      601 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
      661 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
      721 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
      781 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
      841 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
      901 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
      961 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
     1021 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
     1081 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
     1141 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
     1201 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
     1261 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
     1321 tgatcacagg ttcgtttatt gtgacctttt tatctgctat cgttgccacg ccatttgcca
     1381 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
     1441 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
     1501 ttatcgtgcc ttttgtcaga agtatttttg gcgggacagg gtttggtatc ttagctggta
     1561 catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa
     1621 aggcagtacc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
     1681 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
     1741 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
     1801 tgatgccaac aggacttact actcctgctt ctacattgac gtccatctta acaatgggaa
     1861 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
     1921 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
     1981 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atatagtttc
     2041 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
     2101 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gtggtggaat
     2161 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
     2221 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctaacaga
     2281 tgtgattcgc acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt
     2341 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
     2401 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
     2461 ggtccattac acacaacgag aagcaggatt ggccttaggt ttgtcacgct gggaaacagt
     2521 gatgaaagtg gtggttccag aagcgacacc agggattttg actggtgtga tcctaagttc
     2581 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
     2641 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
     2701 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
     2761 tgtttctgtt tcggctggcg cttctgccgt gttgatcatt gctgttttac tgttcaactt
     2821 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
     2881 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
     2941 gaagtagcac tatatacgga tgatatacat gtctggtatg gcgacaatga agcaatcaaa
     3001 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
     3061 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
     3121 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
     3181 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
     3241 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
     3301 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
     3361 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgcat tgctcgtgcg
     3421 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
     3481 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
     3541 gtgacgcata acatgcaaca agcagctcgt atcagcgact atactgcctt tttctatcta
     3601 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
     3661 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagaa
     3721 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
     3781 gatctaagtt tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcggaaaa
     3841 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
     3901 ggaagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
     3961 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
     4021 aatgtcatct atggattgaa attaaaaggg gaaaaagata aaaaagtcct tgatcaagta
     4081 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
     4141 agtgctttat ctttatccgg cgggcagcag cagcgtgtat gtatcgcacg tgttttggca
     4201 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
     4261 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
     4321 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
     4381 ttgatcgaat acaacgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
     4441 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
     4501 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
     4561 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
     4621 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
     4681 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
     4741 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
     4801 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
     4861 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
     4921 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
     4981 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
     5041 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
     5101 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
     5161 gaagctgtga ttatgccgtt tgtttatgaa aagaggttgt gaaaaagctg ttctacatca
     5221 agtaataaga acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat
     5281 taccgcagat gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg
     5341 tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa tgcaaa
//
LOCUS       DAZKMZ010000115         5313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000115 DAZKMZ010000000
VERSION     DAZKMZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.118"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8M5_002530"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8M5_002530"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0359730.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG8M5_002531"
     CDS             complement(1298..2134)
                     /locus_tag="ACG8M5_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359731.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG8M5_002532"
     CDS             complement(2318..3535)
                     /locus_tag="ACG8M5_002532"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0359732.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG8M5_002533"
     CDS             complement(3684..3845)
                     /locus_tag="ACG8M5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359733.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG8M5_002534"
     tRNA            3918..4001
                     /locus_tag="ACG8M5_002534"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5313)
                     /locus_tag="ACG8M5_002535"
                     /pseudo
     CDS             complement(4241..5313)
                     /locus_tag="ACG8M5_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DAZKMZ010000116         5275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000116 DAZKMZ010000000
VERSION     DAZKMZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.119"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACG8M5_002536"
     CDS             171..515
                     /locus_tag="ACG8M5_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0359734.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACG8M5_002537"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACG8M5_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACG8M5_002538"
     CDS             complement(1027..1377)
                     /locus_tag="ACG8M5_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359735.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACG8M5_002539"
     CDS             complement(1343..2674)
                     /locus_tag="ACG8M5_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0359736.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="ACG8M5_002540"
     CDS             2930..3496
                     /locus_tag="ACG8M5_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0359737.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="ACG8M5_002541"
     tRNA            3641..3728
                     /locus_tag="ACG8M5_002541"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="ACG8M5_002542"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="ACG8M5_002542"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0359738.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         1901 a          870 c         1011 g         1493 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaaagat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gaggggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agaga
//
LOCUS       DAZKMZ010000117         5263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000117 DAZKMZ010000000
VERSION     DAZKMZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.120"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..263)
                     /gene="prgO"
                     /locus_tag="ACG8M5_002543"
     CDS             complement(<1..263)
                     /gene="prgO"
                     /locus_tag="ACG8M5_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0359739.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRR"
     gene            complement(235..1188)
                     /locus_tag="ACG8M5_002544"
     CDS             complement(235..1188)
                     /locus_tag="ACG8M5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0359740.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1800..3293
                     /locus_tag="ACG8M5_002545"
     CDS             1800..3293
                     /locus_tag="ACG8M5_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0359741.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3428..3724
                     /locus_tag="ACG8M5_002546"
     CDS             3428..3724
                     /locus_tag="ACG8M5_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0359742.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3825..4676
                     /locus_tag="ACG8M5_002547"
     CDS             3825..4676
                     /locus_tag="ACG8M5_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359743.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4697..4909
                     /locus_tag="ACG8M5_002548"
     CDS             4697..4909
                     /locus_tag="ACG8M5_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359744.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1797 a          820 c          996 g         1650 t
ORIGIN      
        1 cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc gtccattgca
       61 agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg aatggttttt
      121 agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt tcgttcccct
      181 aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt cttcttatct
      241 ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta ggatatgatt
      301 ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac catgttgttc
      361 atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag cccaccatct
      421 tcgatctaca taatcattta attggatacc attctcacca tacatttcta accgatccga
      481 gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg ttttttgata
      541 gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc caatgatttg
      601 aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa taaaatcttc
      661 tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat ctgcagcagt
      721 taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga tataatcgaa
      781 attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac gacgatcagg
      841 atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag ccataatata
      901 taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga ttgctgcaga
      961 catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag tagtatttct
     1021 ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag aaacaatcgc
     1081 taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa gcattacaac
     1141 ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag ttccctccaa
     1201 tttcctatac taaatatatt tagtatactt catatactta atatattcga tatatctaat
     1261 atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat actaaatata
     1321 tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg tgcgttttgc
     1381 gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca gataattcac
     1441 tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca taaaaaaaag
     1501 agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg ttaggacatg
     1561 tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt aaaagttgat
     1621 cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact caacagaaga
     1681 acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga acacatacta
     1741 tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg ttacaatcga
     1801 tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac aaatatcgaa
     1861 acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct atctttgcgt
     1921 accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg gaagaagcta
     1981 ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc tttggtactt
     2041 atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta agtcaaatca
     2101 acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt attttagcct
     2161 gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat ggttatcaag
     2221 tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc aaggtgatcg
     2281 atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa ttgtccggag
     2341 tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat aatattgttt
     2401 ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg atgaagcttg
     2461 aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt cctgagaaaa
     2521 aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa gcaaacatcg
     2581 tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct ttagcttatt
     2641 actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc aacgaacggt
     2701 tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct tattctggta
     2761 attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg gtttcaccaa
     2821 ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt aaaaagccac
     2881 gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg gcttatctaa
     2941 gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa ttggcttcac
     3001 aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga gaggagaata
     3061 aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct tcagtcaaaa
     3121 cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat tcaacaagga
     3181 tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga caattatcaa
     3241 cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt tgaaaaataa
     3301 aaggtgcaaa ttgccctata caatatatct atgatacttc tgtaggtgtt gctccctttg
     3361 cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt atcatggagg
     3421 aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt ttatcattgg
     3481 tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc aggggacttt
     3541 gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt ttcttcatgg
     3601 gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt gtgtgttaga
     3661 gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga agtatatcca
     3721 gtagtagttt ttatttactt aaaaaattgg ttcattgagc aacctatcgc tgctaaagta
     3781 gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa tcggacgact
     3841 ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa ttttgggtat
     3901 tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt cttgatttaa
     3961 ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat cccaatagga
     4021 ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg ctattgtcta
     4081 tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga aaacttttca
     4141 aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata aaacaaattg
     4201 aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt attcactttg
     4261 taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc gggtatgtat
     4321 ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg attccgacac
     4381 ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc aatacggata
     4441 tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt ttaaaaaatg
     4501 gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat gaagtcgaag
     4561 caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt cgtcaaaaca
     4621 aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat aaataattat
     4681 ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg gcatggagtt
     4741 aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc agttactatg
     4801 tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat taacgaatca
     4861 aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat ggctacttat
     4921 agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga acgtctctaa
     4981 aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa aaagtgttcc
     5041 aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata tttaaaaata
     5101 ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc aaaaaggata
     5161 ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa gaataagaaa
     5221 tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gac
//
LOCUS       DAZKMZ010000118         5192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000118 DAZKMZ010000000
VERSION     DAZKMZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.122"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..502)
                     /locus_tag="ACG8M5_002549"
     CDS             complement(44..502)
                     /locus_tag="ACG8M5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359745.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            complement(809..1906)
                     /locus_tag="ACG8M5_002550"
     CDS             complement(809..1906)
                     /locus_tag="ACG8M5_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0359746.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(2014..4041)
                     /locus_tag="ACG8M5_002551"
     CDS             complement(2014..4041)
                     /locus_tag="ACG8M5_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0359747.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4186..4914)
                     /locus_tag="ACG8M5_002552"
     CDS             complement(4186..4914)
                     /locus_tag="ACG8M5_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0359748.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1469 a         1049 c          818 g         1856 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatattgt tataaaaaga gcccaaccat cctacaataa
      781 taaaaattta ctatttgtta tcttaacctt acttttcttt ctcttctttt tttctcaaaa
      841 caaacaaaag tatgcataat aaaataaaca ataagaaaag tgaagctact aagatataaa
      901 tccaattatt gtctttttta atcgtaacat ccgtcgtgtt caattccttg gctacttttc
      961 cgtctattgt aaaatctttt tcaaataccc attgattggt atagttgatc gtagtatttc
     1021 ctttttttct cgaaaatttt ccattttcat tttcatttcc taaaacagtc attgtaaggt
     1081 gatattcacc tggggttagc ggttgaccat ttaaagcagt tggaaaagaa aaatttgtat
     1141 tcggcgcaat ctgcaatcct tctttttcct cttgatataa aacttcagaa tgtccttttt
     1201 ttgtaatttt tgtttcaata actacttgat tgatataggt tttttgatca ttttgaacgt
     1261 ttgctaagat gacatttctt gcattgattt gatctggttt aacttcatga agagtaaggt
     1321 tcggagcaac ttcgttcagt gtctgttgca tcaacaaagc aataatatag gtgtattcat
     1381 tctcaatcga taagctttga tccttagttt ctgtttggtc cttttccgtc tggatttctt
     1441 ggaatgtgat cccaccagcc aatacaccat caaatcgctc attcggcatc ctgacatgaa
     1501 aagcaacggt ttggacactt ttaggtttta gcgtcactat ctgtggatag ctcacataat
     1561 ctaccagatc aaagatcagg ctcatgtctt tttcaatctc gttttttccg tattcaacca
     1621 tcacatttga attggtggtt gcgctattca cagaaatatc gattttcact tctttttctg
     1681 tatcatttct caattccgct tttacttcta cttcctgatc cggatcaagt tgaagatcga
     1741 agtaagtttt tctcttgtcg atttgattct ctgaagttat cggcgtcact gaaaaattaa
     1801 atgcactagc tgacactgat atattgttaa aaagtgcccc taaaataact aggagtgtta
     1861 taaataattg ttgttttggt ctcaagaaaa tcgctccttt aatcatatgt aagagaagac
     1921 tataacaaaa tcttgtctct gtgggacaca agagcctatg gtacctgtgc ccttcttgaa
     1981 actcaataat tttgttatat tcttctcttc ctgttaattt cctgggacat ctgcaagtat
     2041 ccaagatagt ttcccctcaa aggttccgat tctttggttt tctacaggaa tatttattct
     2101 gattccacga tgagctgtag aatctaaaag agaggacaaa tcgctttcac ccttttgatc
     2161 actttcgaaa atcgtaacag cggatgtccc tataaccgtt gtattttcag aggtaacaaa
     2221 ggaaagagct tctggaagct caaaatctcc atttgtaaga actttctctt gtttcaattg
     2281 taatttccaa cttccaaaag cccgttcatc tataattttc aacccacctg aaaactgtcc
     2341 gaattcactt aatggtctac tggatatttt atgttttcca aaattgacat attgaggagc
     2401 agttgagaga taaagtttgc cttttctgta ataaaaatag atatcctctg tattttcgga
     2461 gaagatgcct tctgtattcg ctgaatccga gtcaaaaaca tacccttcaa tatcaatagg
     2521 agtttcttta tacttctctc ctattttccc agtgattgtt ttcatttcag caatatcaat
     2581 ctgactgtct ttatctttat aataaacatg gatatctatc tccttcagct ttgcgatctt
     2641 taggtttatt tcattcgatt gctcaatcaa attcccgttt tcaaggtatt caaaatgcat
     2701 agtgagtact ttttcaggaa ttacttcctg caaagttgtc tccaattcga ttattaacgt
     2761 atctccagca ataaaataat ctgtcagatt tagcgtgact cgatttccgg ttatcaagat
     2821 atctttggca tcaatttgat ttccattgat caagatatgc tcgggaatag ttaattcttg
     2881 tgggaggtcg atactagtct gcattttctt atattcaaga aaatccccct cgaccttcaa
     2941 tgtatttctg atagttatct tatcgtttcc tccagcttca gcgatggccc cataatcact
     3001 atcgttgact tttacatcag ctgttgatgt gacattcgat ggaagtcgcg tgacagcgac
     3061 agcattttct tgaaaaaaag tacctgttga agaagtaaac ccccaattta cttctgtaga
     3121 accaaactgc ctaatgagat caggtataat ccgagaagag cgaccgacac cttccaaatc
     3181 ataagtaaga cttctttgct ccgcgtccca tttaattatt aacaagcgcc atgtgccatt
     3241 agaaagaaat gtaggagcta ccgtcacgcc agtatgctct ccagaataat tattattatt
     3301 tggtttaggc gtgacaaaag cgacatgtcc atgccctcta ttatcagcag ttatctctcg
     3361 atccattcga tcagaagaac cactattttt atacgtatca aactcaatcg agagcgcatt
     3421 cgcaatgtaa ggttggccag aacgagtaga atatgagcca attcccatac ctggcgatcc
     3481 aataacttga ctgggattac tagccattct aggatcgcca gtcaatgtaa aggtcatgcc
     3541 atctcccgct ctatctcgtt gatttcctaa gtataaataa gatttaaatg taaaatccga
     3601 gagtaaacta atcttcttat ttgaccaaat agctcctctt tgagaccttt ctgctgggtt
     3661 caactgcagt aacttcccat tttccaatat ggacgtgcta gtaccgatag gtgtttgaaa
     3721 aattttatcg atagaaataa tattgggatc gtcaggcagc tcaaccgcag aaattggtgc
     3781 atcttctagt ccttgtttgt ccgaaccctc agcttctacg ttgtcagtgg tctctccact
     3841 actcgaaata cttaaatcct cactgctttc ttgtgtctgg ttggattcta ttaaactgtc
     3901 gaatgtcgaa gtgagtttgc tttcttcgtc tcttctattt ccatgctcca actgattcaa
     3961 ttccaaagag taccctatga tcggaaagat attagtcact aagagactgc ttaaaatgag
     4021 cacgatcctc ctctttttca atatatcttt tcctcctgat cgttaaattc ctagatgaac
     4081 tattcgtatc caaacaaatt tagcctagcc tttgacaatt tttgctcgat tacctcgcaa
     4141 gacaatatga ctacgtattt atttttaaca gttcttttca gtaaactagt tgttaggtgt
     4201 atcttctaat gtccaaacta atttagtgga atattgttgc gctaacttga cagatttacc
     4261 gggtaccgaa agctgtaccg catcttgatt ttcattttca tttgcaccaa atcgatagac
     4321 ccaagtcccc acacctgttc ctgatttagc attaattgct aacgtattgt ttcctcccgg
     4381 tgtcaacgtt aattcagaat tcaccgtttg cggcgtatat gttggatcaa ccaaagaaac
     4441 agcctgccct ttcgtaaatt tcagctgtgc tccaactagt tcatccccac tggcagtttt
     4501 aaactgttct ggttgagaga cacttaattt ccacccagat aaggtacttc tcctatctgt
     4561 cacttggaca tatgtcggtt ttctagaccc atcgttcatc acatcagcag ctgcataata
     4621 cgttttatcc gcagtagaaa ttttttgagt cccaaatttg aattttgatc cataatcaat
     4681 cgataatgcc cctcctgttc cagggcttgg aggatctgat ggatcgacag gtttattggg
     4741 atcaggattc tctggatcaa caggaggagt tacaccctca tctcctgctt caaatgtaat
     4801 cgttccttct gtgttatatt cctttgggta tacttcagtt gcagaaacaa aagtcgtact
     4861 tgctaccatg ctgaataaca tcattgctgc tgttaatacg atttcttttt tcatcattga
     4921 cattcctctt tctttattta ctaaacatat cagtcgattg gattaatcca tagcaaaatt
     4981 ctgttttacg atacttatga aataaaagaa catagaccta tatttggtcc tatgtatcga
     5041 ttagttataa atgattatca gcccgatttg atttctagtt tttattgcaa cttgatgcaa
     5101 acacaatcta gaaatacagc tgtcatagcc tttaaaataa ttcgtaagtc cccctcctat
     5161 ctatcaaaat gtgacttgac gagagtgtaa aa
//
LOCUS       DAZKMZ010000119         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000119 DAZKMZ010000000
VERSION     DAZKMZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.123"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8M5_002553"
     CDS             137..748
                     /locus_tag="ACG8M5_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0359749.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8M5_002554"
     CDS             821..1750
                     /locus_tag="ACG8M5_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0359750.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8M5_002555"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8M5_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8M5_002556"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8M5_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0359751.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8M5_002557"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8M5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8M5_002558"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8M5_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0359752.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1638 a          879 c         1004 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 gtgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKMZ010000120         5184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000120 DAZKMZ010000000
VERSION     DAZKMZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.124"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..1387
                     /locus_tag="ACG8M5_002559"
     CDS             218..1387
                     /locus_tag="ACG8M5_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0359753.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1513..2592)
                     /locus_tag="ACG8M5_002560"
     CDS             complement(1513..2592)
                     /locus_tag="ACG8M5_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0359754.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2701..3141)
                     /locus_tag="ACG8M5_002561"
     CDS             complement(2701..3141)
                     /locus_tag="ACG8M5_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0359755.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3198..3608)
                     /locus_tag="ACG8M5_002562"
     CDS             complement(3198..3608)
                     /locus_tag="ACG8M5_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0359756.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3789..4223)
                     /locus_tag="ACG8M5_002563"
     CDS             complement(3789..4223)
                     /locus_tag="ACG8M5_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0359757.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4323..5171)
                     /locus_tag="ACG8M5_002564"
     CDS             complement(4323..5171)
                     /locus_tag="ACG8M5_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0359758.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1889 a          987 c          871 g         1437 t
ORIGIN      
        1 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
       61 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
      121 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
      181 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
      241 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
      301 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
      361 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
      421 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
      481 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
      541 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
      601 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
      661 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
      721 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
      781 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
      841 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
      901 agaagaaaaa ccggatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
      961 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaactag aggtagatcc
     1021 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
     1081 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
     1141 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
     1201 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgcta gtcctgaaac
     1261 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
     1321 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
     1381 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
     1441 aacgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
     1501 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
     1561 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
     1621 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
     1681 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
     1741 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
     1801 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
     1861 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtgtt
     1921 taaaagagcg ataacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
     1981 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
     2041 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
     2101 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
     2161 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
     2221 atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt
     2281 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
     2341 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
     2401 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
     2461 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
     2521 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
     2581 cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa
     2641 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
     2701 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
     2761 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
     2821 tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc
     2881 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
     2941 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
     3001 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
     3061 ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt
     3121 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
     3181 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
     3241 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
     3301 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
     3361 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
     3421 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
     3481 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
     3541 tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
     3601 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
     3661 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
     3721 cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat
     3781 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga
     3841 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
     3901 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
     3961 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
     4021 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca
     4081 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
     4141 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
     4201 tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
     4261 attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca
     4321 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
     4381 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
     4441 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
     4501 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
     4561 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
     4621 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca
     4681 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
     4741 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
     4801 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
     4861 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt
     4921 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
     4981 acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat
     5041 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
     5101 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
     5161 gtttctttca tttatccact tcct
//
LOCUS       DAZKMZ010000121         5182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000121 DAZKMZ010000000
VERSION     DAZKMZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.125"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..366)
                     /locus_tag="ACG8M5_002565"
                     /pseudo
     CDS             complement(1..366)
                     /locus_tag="ACG8M5_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            531..2893
                     /locus_tag="ACG8M5_002566"
                     /pseudo
     CDS             531..2893
                     /locus_tag="ACG8M5_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3261..4184
                     /gene="truB"
                     /locus_tag="ACG8M5_002567"
     CDS             3261..4184
                     /gene="truB"
                     /locus_tag="ACG8M5_002567"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0359759.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4188..5129
                     /gene="ribF"
                     /locus_tag="ACG8M5_002568"
     CDS             4188..5129
                     /gene="ribF"
                     /locus_tag="ACG8M5_002568"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0359760.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         1801 a          905 c         1098 g         1378 t
ORIGIN      
        1 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
       61 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
      121 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
      181 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
      241 gaattgctcg atccagcccc agttttccag caatgtgtta actggcccag cgtcattcag
      301 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
      361 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
      421 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
      481 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
      541 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
      601 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa cccgaagatg
      661 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
      721 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatattgaa gggccaggac
      781 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
      841 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caaacgattt tcatttcctg
      901 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
      961 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
     1021 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
     1081 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
     1141 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
     1201 ttaacggact tggctgggaa cctttcttta ttgaaggaaa tgattcagaa aaattaaatc
     1261 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat taaatccatc caaaaagaag
     1321 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
     1381 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
     1441 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
     1501 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
     1561 aagaaatcag agaaatcgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
     1621 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
     1681 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
     1741 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
     1801 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
     1861 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
     1921 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt ttgcaagtta
     1981 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatgcggaa
     2041 atcacatgac ctttcttggc gaaatgatta tccttcgcta aatctcattg caagctcaac
     2101 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
     2161 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
     2221 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
     2281 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
     2341 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
     2401 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
     2461 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
     2521 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
     2581 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
     2641 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
     2701 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
     2761 caaaggaagt caagcagaag attttgcgaa aaaaatggat cagaaattac aagaacatca
     2821 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
     2881 tcttgaccaa taaaatgata aaatcgatcc cgcaaaatag aattttttac attgattaag
     2941 aaactgaaaa aataaataaa aaaaacggaa aaaagactgg aagaatggcc agtctttttt
     3001 ttcgtttttg ggaagtgaaa accaaactat ttacctgtaa gccacccata aatccaatca
     3061 aatggtgcaa agaaaaaatc ttccattcta ttccctcctt taaaaaagca taacatgatt
     3121 tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa aaaccgttca
     3181 ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca gttaaatcaa
     3241 aaatgaaatg gagaaaagac atggacgggt tattaccttt atggaaagaa cgcggaatga
     3301 caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa atcggacatg
     3361 ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga aaagccacaa
     3421 aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata actctaggtt
     3481 tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca gtagatatgt
     3541 ctttatctga aaaaaaaata gatgaagtga tggcttcatt tattggtgag atcacccaga
     3601 taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa tatgcccgca
     3661 ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt atccggacaa
     3721 gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa gtagaatgcg
     3781 ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt ggttaccctg
     3841 cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaag gatgctctga
     3901 cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt ttgctgccaa
     3961 tagaaaaagg agtggaacgg ttcaaacgag tagatattac tgatgaagta tggcagaaag
     4021 taaaaaacgg tatgagactt gattatactg tatttgcgct cccagaaatg ccgtcagaag
     4081 aaattgcttt gttttataaa ggaaaagttg tcagtattta tcagccaaac ccgaaagaaa
     4141 aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg aatatcatca
     4201 agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt ttagtgctgg
     4261 gatttttcga cggcgtgcat atcggacacc agaaagtcat aaagacaggt atggaaattg
     4321 cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca tctattgtct
     4381 ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa aaagaagaaa
     4441 aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct tcttttgctc
     4501 atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca aaatatgctg
     4561 tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct cattttcctg
     4621 aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa caaggtgaaa
     4681 aaatcagctc tacccgtatt cgtggattac tagacagcgg acaagtaaaa gaagcagcaa
     4741 aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat gcacgaggca
     4801 gactactagg attccctaca gcaaatgtca aagtaaagag cacggttcat ttgcctaaag
     4861 aaggtgttta tgtgtcggaa ataaaaattg gggaaacatg gtatccagcc atgggttcaa
     4921 ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagagttg tatattttgg
     4981 actttaatca agatatctat ggtgaacatg tcgcggtacg ctggaatcaa tttttaagag
     5041 atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaggag gatgaacaag
     5101 caacagctga atatttttct cagcaataaa acattatgca taaaatctct tgtttatgta
     5161 taactagcaa ttacaataaa at
//
LOCUS       DAZKMZ010000122         5176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000122 DAZKMZ010000000
VERSION     DAZKMZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.126"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8M5_002569"
     CDS             complement(136..1098)
                     /locus_tag="ACG8M5_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359761.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8M5_002570"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8M5_002570"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0359762.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8M5_002571"
     CDS             2724..4670
                     /locus_tag="ACG8M5_002571"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0359763.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACG8M5_002572"
     CDS             4934..>5176
                     /locus_tag="ACG8M5_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0359764.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZKMZ010000123         5172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000123 DAZKMZ010000000
VERSION     DAZKMZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.127"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..700)
                     /locus_tag="ACG8M5_002573"
     CDS             complement(38..700)
                     /locus_tag="ACG8M5_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0359765.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(710..1525)
                     /locus_tag="ACG8M5_002574"
     CDS             complement(710..1525)
                     /locus_tag="ACG8M5_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0359766.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1787..2230)
                     /locus_tag="ACG8M5_002575"
     CDS             complement(1787..2230)
                     /locus_tag="ACG8M5_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0359767.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2364..2702)
                     /locus_tag="ACG8M5_002576"
     CDS             complement(2364..2702)
                     /locus_tag="ACG8M5_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0359768.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2690..3067)
                     /locus_tag="ACG8M5_002577"
     CDS             complement(2690..3067)
                     /locus_tag="ACG8M5_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0359769.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3291..4484
                     /locus_tag="ACG8M5_002578"
     CDS             3291..4484
                     /locus_tag="ACG8M5_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0359770.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4650..5072)
                     /locus_tag="ACG8M5_002579"
     CDS             complement(4650..5072)
                     /locus_tag="ACG8M5_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359771.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1568 a          986 c          890 g         1728 t
ORIGIN      
        1 actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca
       61 atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc
      121 attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac
      181 tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg
      241 acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag
      301 caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac
      361 aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta
      421 gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg
      481 ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa
      541 aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca
      601 agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga
      661 attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca
      721 ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa
      781 caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc
      841 caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga
      901 atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg
      961 ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga
     1021 tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca
     1081 caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca
     1141 cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc
     1201 cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag
     1261 cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat
     1321 tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc
     1381 aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag
     1441 cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca
     1501 tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat
     1561 ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac
     1621 ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt
     1681 tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc
     1741 atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag
     1801 cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc
     1861 agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg
     1921 aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg
     1981 caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc
     2041 ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat
     2101 tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga
     2161 gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg
     2221 gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac
     2281 ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta
     2341 atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga
     2401 ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt
     2461 cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg
     2521 cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt
     2581 ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc
     2641 caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac
     2701 attccatttt atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt
     2761 ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct tcaatcaaaa
     2821 gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca
     2881 aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt
     2941 cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga
     3001 ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga
     3061 tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat
     3121 tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta
     3181 tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt
     3241 tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt
     3301 atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat atggatatta
     3361 tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag
     3421 cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca
     3481 tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga
     3541 ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta
     3601 tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt
     3661 tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg
     3721 cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt
     3781 atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac
     3841 caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg
     3901 aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg
     3961 ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt
     4021 cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga
     4081 tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa
     4141 tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag
     4201 cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga
     4261 ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg
     4321 gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt
     4381 taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa
     4441 ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa
     4501 tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa
     4561 aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca
     4621 actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat ttcaaagtac
     4681 tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca
     4741 taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat
     4801 gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg cttttccata
     4861 gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat
     4921 tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt
     4981 agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc
     5041 ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta
     5101 tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca
     5161 taaacctgtt at
//
LOCUS       DAZKMZ010000124         5123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000124 DAZKMZ010000000
VERSION     DAZKMZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.128"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1011
                     /gene="gnd"
                     /locus_tag="ACG8M5_002580"
     CDS             <1..1011
                     /gene="gnd"
                     /locus_tag="ACG8M5_002580"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0359772.1"
                     /translation="KDTRRVGKNQTKNIFVKSIYKKIFLYNEGIRNGGAIMEIGVIGL
                     GKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAYQEISEFLATFEQKKIIWLM
                     LPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRAEEFQKNEIGYLDCGTSGGI
                     EGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGASGSGHFLKMIHNGIEYGMM
                     QAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEIMEDQLNEHPNLSNYRGIVS
                     ASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFSAKVVSALRNGFGGHEIVEK
                     GE"
     gene            1013..2014
                     /locus_tag="ACG8M5_002581"
     CDS             1013..2014
                     /locus_tag="ACG8M5_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0359773.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            2011..2295
                     /locus_tag="ACG8M5_002582"
     CDS             2011..2295
                     /locus_tag="ACG8M5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0359774.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2328..3125
                     /locus_tag="ACG8M5_002583"
     CDS             2328..3125
                     /locus_tag="ACG8M5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0359775.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3139..3972
                     /locus_tag="ACG8M5_002584"
     CDS             3139..3972
                     /locus_tag="ACG8M5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0359776.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3990..4847
                     /locus_tag="ACG8M5_002585"
     CDS             3990..4847
                     /locus_tag="ACG8M5_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0359777.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4869..>5123
                     /locus_tag="ACG8M5_002586"
     CDS             4869..>5123
                     /locus_tag="ACG8M5_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0359778.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIA"
BASE COUNT         1660 a          820 c         1175 g         1468 t
ORIGIN      
        1 aaagatacaa ggagagtagg aaaaaatcaa acaaaaaata tttttgtaaa atctatttac
       61 aaaaaaattt ttttgtataa tgagggtata agaaatggag gcgctatcat ggaaattggt
      121 gtaattggtt tgggaaaaat gggattgaat ttagctttaa acttaaaaga gcaccatcac
      181 aaagtacaag gactcgacat ttcaagtaca gctgttagtc aggcaagaga aaatgacatt
      241 cctgcttatc aagagatctc tgaatttcta gctacttttg agcagaaaaa aatcatctgg
      301 ttaatgcttc ctgctggaga gataaccgaa aacatgttaa gaaaactttt tccaaaactc
      361 ggaccaggag acattattat tgaaggcgga aactcttatt ataaagattc aatccgtcga
      421 gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt gtgggacttc tggaggaatc
      481 gaaggagcac gacatcaagc ctgcttaatg attggaggag ataaagaaac ttttctatcc
      541 gtggaacaat tattcaaaga tgtagcaatt gaaagaggat atctgtatgc tggtgcatcc
      601 ggaagcggac acttcttgaa aatgatccat aatggaatcg aatatggaat gatgcaagca
      661 atcggtgaag gattccaact tgtacaaaaa agtgagtatg cttttgactt ggaacaagtc
      721 gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc tgatggaaat catggaagat
      781 cagttaaacg aacatcccaa tttaagtaat tatcggggaa tcgtttctgc atcaggtgaa
      841 gcaaaatgga cggttgaaac agcgctagaa atggctgttc cagttccagt aattgcaact
      901 agtttgttca tgaggaatct ttcacaggaa gaagatagtt tttcagcaaa agtagtttca
      961 gcactaagaa atgggtttgg cggacatgaa atagttgaaa agggtgagta aaatgccaga
     1021 ttatcatata gcaatcgtta attccagcag tttcggaaga gtctttccac accacttgga
     1081 aagattaaag tcagttggag acgttcattt ctttactgtt gatcaagaga ttccaggaaa
     1141 agaactagcg gagttactta aaggattcaa tatcattatt gcaagtgtta cgccattttt
     1201 tcgacgagat ttttttgaaa acaaagatga actgctattg atttcccgac atggtattgg
     1261 ttacaacaat attgatttaa aagcagcaaa agaacaccag acaatcgtat ctatcatacc
     1321 tgctttagtt gaacgaaatg ctgtagcaga aaataatata actaatttgt tgtccatgct
     1381 tcgacgcacg acacaagcac aagcaagagt caaggacgat cgatgggaag atcgggcgga
     1441 gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg attggtgcag gaaataccgg
     1501 aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt gatgttctag cttatgatcc
     1561 ttacaaatca gatttataca tcgaaagctt tggtgcaaaa aaagtagaac ttcaagaatt
     1621 actggaacaa tcagatatca tttgtttatg tgcgaatttg acagaagaga actatcacat
     1681 gatttctgat aaagaagtcg atcagatgaa agaaaatgtt tatctatcta atagtgcaag
     1741 aggcgctcta gttgatgaag aagcgatgat taatggtctg aaaagcggaa agatcgcagg
     1801 atatgccaca gatgttttag aagaagaacc gggacgtcaa gatcatccgt atttagcgtt
     1861 tgaacaagta gtgattacgc cacatatatc ggcctacaca agggaatgtt tagaagcaat
     1921 gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag caccttcttc ctgaacgttc
     1981 tgtccagaca aagagcaact atataaaagg atgatgaaga aaatggtgaa cggtttaaaa
     2041 gtgaaaacag gccctcaatt ttatttatat gaagaaggtg gtatttcaaa agtttctgat
     2101 ctcttgaaat catacggcgc gaaaagagta ctggtcaccc acggaacggt atcatgggaa
     2161 aaggcgctac ctaaattagt atttttgaat gacgaaacga ttcaattttt ttatcatcgt
     2221 tattctggag aatgtagtta tgctgaagcc agacgaatag ctacaatcat caaaaaaatg
     2281 aaatcgattt cgtagttggc gttggcggag gaaaactgac cgatctagtg ggttatagtg
     2341 ccaatcttgc aagaatcgat ttcggagtca tccctacttt agctagtaat tgtgctccat
     2401 ggacacctct tgctgtcatg tataaggaga atggacaagc agaagggaaa acagaacact
     2461 tttacagaca agctgctttt tttataacag atccccaatt agtgattgat tcccctgttc
     2521 catatttcat tgccgggtta gcagatacac tagcaaaatg gtatgaatct gagctcatct
     2581 tagaacaaga ttttttgcaa gatgaatcat ttttgaagct agcacaagat actgcgaaga
     2641 tctgcaaaga agagattttg aatcattctg ctaaagcaat ccaagatatg gcacaaagaa
     2701 aactgacatc ggagttcaaa cagctttcag agattatctt tgctgttgct ggtttagttg
     2761 gaggattagg agataaatac gcaagaaatg tggcagcaca tgctatgcat gacgctatca
     2821 gtaaatatat tccggaaagc catcgttttc tacatggaga aaaagtagcg tatgggattt
     2881 tctatcaact ggcactagaa ggtcgctggg aaaaaataga cgagttatat cctttgtatc
     2941 gggaattaga actgccggtt tctctagaac aaatgggatt atttccgaaa gataaacaga
     3001 taatagataa aatggctgaa tttattgatt cgaaagagaa agtgcatttg attcctatag
     3061 aggtaacaca gaaatcactg atacagtcaa tgatggaact ggaaggttat atcagaaaga
     3121 tctgaaggag gaggacacat ggaaacatta agtgtagtac aaagtttgtt gattgcttta
     3181 tgggtagccg cggttatgtc ccgctggtta ggtggaggag ccacgttgac cttacgtttt
     3241 tctcctttga tgactggatt agtcgtcggg ttgatcatgg gggaagttgc acaagcgatg
     3301 attgttacag ccgctttgca aatgatttat atgggggttt tttctccagg aggatcaatg
     3361 ccagctgaac catctattgc agcagctatc gcagtaccag tcgcattatt aggggatctg
     3421 tcgccagaag cagcgattgc cgttgcggtg ccagtcggat tgctcggaag ttacttgtat
     3481 cagtttcgtt ttttcatcaa tactttttta ggtaaatata cagacaaagc tgtagaagat
     3541 ttgaatacaa aagctatggc ccgatcaatt attctttatc caacggttgc ctcattttta
     3601 ttattcgttc cgcttgtttt cattgcttta tatttagggg cgccattgat cgctgatgta
     3661 atcaaagctt tagagggaac agtcgttatc cacattcttg aagtagtagg tggagggtta
     3721 gcggcaatcg gtatcgcgac aactgtatac gttatcggtc gaaaagatta tctggtcttt
     3781 ttcttccttg cttattttat gagtgttgtt ttgaaatctt tagatattac gatggttact
     3841 tatgccattt ttgggattat tatcgctttg atctttgtcc agtcgcaaaa agggaaacaa
     3901 gtacagcctg ccacaagcaa taacaattca gcatcttttg atgacgaaga tgactatgat
     3961 gacggatttt aatttaggag gaatgggaaa tggctgaaat aatggataat caaacaggtg
     4021 ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta tctgagatgg cattttgcaa
     4081 acgaaattcc tcactctttt gaacgttact tggcgccatc actactatac ggaatgatgc
     4141 caatcttgaa aaaactttat aaagatgaag actctttaaa agcagcatat aaacggcagt
     4201 tactgttctt caacactcaa ttaagctggg gtggcggtgt gattactggc ctaatgtctt
     4261 ctatggaaca gcaaagagca gaagaagagt atgaagaaaa agagatcttg atgcaggatg
     4321 acttgatgta taacacgaaa gcgggattga tgggggcact agcaggaatc ggagatgcaa
     4381 tcgattcagg aaccgtccaa tatattttta tcgcaatcgc ggtaccttgg gcacaacagg
     4441 gaagtccttt aggcgcactt tttcctttta tctgctttgg gttgtatcag gttttactag
     4501 gggtattttt cgcaagacaa gctttcacta tgggccgtaa tgctacaggc ctaaagcaaa
     4561 acactggtgt tcagacagcg attgaaatgc tatcagtttt agggttgttt atgatggggg
     4621 tgcttgcggg aaactatgta aaagtagaat cttcattgaa atttgcgata tccggacgtg
     4681 agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg gatattacct ttagctgtcg
     4741 taatgggtgt gtacttgttc tatacgaaaa aaggattgaa agtcacacaa gcattattat
     4801 ggctgactgg aatattaatt gtgctagcag gaatcggtat tttataaaaa gaagggaacg
     4861 tgaataagat gggtgtagtc aatttagcgc gtgtggatga gcgtttgatc catgggcaag
     4921 taatggtaac gttatctcaa aaaagtggag tgaattccat ttttgttgta gacgaagttg
     4981 tagcaaaaga taaatttatg agagatcttt acaaaaatgc aggaagccgt acaggacaaa
     5041 aaacaattgt cattacacca gagaaagcta aattctattg ggatgaatat caatttaaag
     5101 aatacaattg tattttgatc gca
//
LOCUS       DAZKMZ010000125         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000125 DAZKMZ010000000
VERSION     DAZKMZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.129"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8M5_002587"
     CDS             85..972
                     /locus_tag="ACG8M5_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0359779.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M5_002588"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M5_002588"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0359780.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8M5_002589"
     CDS             2274..2720
                     /locus_tag="ACG8M5_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0359781.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8M5_002590"
     CDS             2993..4345
                     /locus_tag="ACG8M5_002590"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0359782.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8M5_002591"
     CDS             4516..4803
                     /locus_tag="ACG8M5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0359783.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKMZ010000126         4892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000126 DAZKMZ010000000
VERSION     DAZKMZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.130"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8M5_002592"
     CDS             complement(287..649)
                     /locus_tag="ACG8M5_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359784.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8M5_002593"
     CDS             complement(723..1103)
                     /locus_tag="ACG8M5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0359785.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8M5_002594"
     CDS             1185..2360
                     /locus_tag="ACG8M5_002594"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0359786.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8M5_002595"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8M5_002595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0359787.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8M5_002596"
     CDS             3222..4220
                     /locus_tag="ACG8M5_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0359788.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8M5_002597"
     CDS             4332..4841
                     /locus_tag="ACG8M5_002597"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0359789.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAZKMZ010000127         4665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000127 DAZKMZ010000000
VERSION     DAZKMZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.131"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..1644
                     /locus_tag="ACG8M5_002598"
     CDS             154..1644
                     /locus_tag="ACG8M5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0359790.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            1992..2162
                     /locus_tag="ACG8M5_002599"
     CDS             1992..2162
                     /locus_tag="ACG8M5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359791.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2176..2793
                     /locus_tag="ACG8M5_002600"
     CDS             2176..2793
                     /locus_tag="ACG8M5_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359792.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2793..3731
                     /locus_tag="ACG8M5_002601"
                     /pseudo
     CDS             2793..3731
                     /locus_tag="ACG8M5_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4424..>4665)
                     /locus_tag="ACG8M5_002602"
     CDS             complement(4424..>4665)
                     /locus_tag="ACG8M5_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0359793.1"
                     /translation="NTIPISMIPWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIY
                     IPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
BASE COUNT         1710 a          728 c          852 g         1375 t
ORIGIN      
        1 aaaataattt attcgattag tggaaaaaaa ttgacttata aaggaaaaaa atctttttca
       61 aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc aagttaatta aacaacctat
      121 tttataggat ttataggaaa ggagaacagc tgaatgaata tcccttttgt tgtagaaact
      181 gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata gtaaaattcg ctcaatcact
      241 accaagccag gtaaaagcaa aggggctatt tttgcgtatc gctcaaaatc aagcatgatt
      301 ggcggtcgtg gtgttgttct gacttccgag gaagcgattc aagaaaatca agatacattt
      361 acacattgga cacccaacgt ttatcgttat ggaacgtatg cagacgaaaa ccgttcatac
      421 acgaaaggac attctgaaaa caatttaaga caaatcaata ccttctttat tgattttgat
      481 attcacacgg caaaagaaac tatttcagca agcgatattt taacaaccgc tattgattta
      541 ggttttatgc ctactatgat tatcaaatct gataaaggtt atcaagcata ttttgtttta
      601 gaaacgccag tctatgtgac ttcaaaatca gaatttaaat ctgtcaaagc agccaaaata
      661 atttcgcaaa atatccgaga atattttgga aagtctttgc cagttgatct aacgtgtaat
      721 cattttggta ttgctcgcat accaagaacg gacaatgtag aattttttga tcctaattac
      781 cgttattctt tcaaagaatg gcaagattgg tctttcaaac aaacagataa taagggcttt
      841 actcgttcaa gtctaacggt tttaagcggt acagaaggca aaaaacaagt agatgaaccc
      901 tggtttaatc tcttattgca cgaaacgaaa ttttcaggag aaaagggttt aatagggcgt
      961 aataacgtca tgtttaccct ctctttagcc tactttagtt caggctattc aatcgaaacg
     1021 tgcgaatata atatgtttga gtttaataat cgattagatc aacccttaga agaaaaagaa
     1081 gtaatcaaaa ttgttagaag tgcctattca gaaaactatc aaggggctaa tagggaatac
     1141 attaccattc tttgcaaagc ttgggtatca agtgatttaa ccagtaaaga tttatttgtc
     1201 cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac gtcaacgtgt tcatttgtca
     1261 gaatggaaag aagatttaat ggcttatatt agcgaaaaaa gcgatgtata caagccttat
     1321 ttagtgacga ccaaaaaaga gattagagaa gtgctaggca ttcctgaacg gacattagat
     1381 aaattgctga aggtactgaa ggcgaatcag gaaattttct ttaagattaa accaggaaga
     1441 aatggtggca ttcaacttgc tagtgttaaa tcattgttgc tatcgatcat taaagtaaaa
     1501 aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt cttttgactt agagcataca
     1561 ttcattcaag agactttaaa caagctagca gaacgcccta aaacggacac acaactcgat
     1621 ttgtttagct atgatacagg ctgaaaataa aacccgcact atgccattac atttatatct
     1681 atgatacgtg tttgtttttt ctttgctgtt tagcgaatga ttagcagaaa tatacagagt
     1741 aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact tttagttggc
     1801 ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg agagcgaagc
     1861 gaacacttga ttttttaatt ttctatcttt tataggtcat tagagtatac ctatttgtcc
     1921 taatatgatt ttagcagtat aattgacttg gtgaataggt catttaagtt gggcataata
     1981 ggaggagtaa aatgaaaaaa tttatttatc gagttttaga aaatgacgaa gtggtggcta
     2041 tttttaatga gcaacaatat gcgcaagatt ttatcgctta cgaaaagaca atttctgata
     2101 agcaatttga aattgaaaaa gtagatattg ctgattggtt attgcaaccg agagaatttt
     2161 agaggttggt tgaaaatggc taaaattggt tatgcacgtg tcagtagcaa agaacagaac
     2221 ttagatcggc aattacaagc gttacagggc gtttctaagg tcttttcaga caaattaagc
     2281 ggtcaatcgg tcgaacgccc acaattacaa gctatgctta actatattcg tgaaggggat
     2341 attgttattg ttactgaatt agatcgatta ggacgaaata ataaagaatt aacagaattg
     2401 atgaatcaaa ttcaaattaa gggggcaacc ctggaagtct taaatttacc ctcaatgaat
     2461 ggtattgaag atgaaaattt aaggcgtttg attaatagcc ttgtcattga attgtacaag
     2521 tatcaagcag aatcagaacg aaaaaaaatt aaggaacgtc aggcacaagg aatcgaaatt
     2581 gctaagaaaa aaggcaaatt caaaggtcgt cagcataaat ttaaagaaaa tgatccacgt
     2641 ttaaagcatg ctttcgattt gtttttgaat ggtttatccg ataaagaagt tgaagaacaa
     2701 actggaatca atcgccgaac gtttagaagg tatcgagcaa gatataacgt gacagtcgat
     2761 caaagaaaaa acaatgaaaa gagggatagt taatgagtac ggttatttta gctgaaaaac
     2821 caagccaggc attagcctac gcaagtgctt taaaacaaag caccaaaaaa gacggttatt
     2881 ttgagatcaa agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa agcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtaatc cgaagcggtt
     3241 ttcaaaattt gcaacctgga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctagtataaa agtaaaccaa gggtcgttta agggcgttct aagccccaca cagcgtttta
     3541 aaacccaaga ggagctttta gcttttgttt cttctaaaca agctaaaata ggcaatcaag
     3601 aggggataat tgctgatgtt caaaccaaag agaagaaaac gaatagtccg agtttgtttt
     3661 ctttaagtag tttgcaatca aaagtcaatc agctttataa agcgacagcg agccaaactt
     3721 taaaagctat ttctttttta ataacttaaa aataaactta atgtaacagc aagcacagtc
     3781 aaggtataca cctttgacaa aaaatagcac attctctatc gaaaattttt gcttattttt
     3841 taaattattt tgggaaattt tcccaatccc tttttctaac tcaaaaaata taatcactca
     3901 aaatttaaaa gggcgcactt atacatcatt ttaaaaaatt gatgtaacgt gctaagttca
     3961 aaacaaaggg cgcacttata cacgattttc aatcttgtat atttctaacg aaaagcgtgc
     4021 gccaaaaaac ccccttcgtc aattttgaca gggggctttt tgatgtaaaa atttctatcg
     4081 aaatttaaaa attcgcttca ctcatgttat aaagacttaa aataaaataa ctctttaaaa
     4141 tcttttgcta gttgttcttc aatatttttt attcggtgca tcttccaagt aaagtataac
     4201 acactagact tatttactac gtttcataag tcattaatgc gtgtgctctg cgaggctagt
     4261 ttttgtgcaa gcacaaaaaa tggactgaat aaatcagtcc ataagttcaa aaccaaattc
     4321 aaaatcaaaa ccacaagcaa ccaaaaaatg tggttgttat acgttcataa attttatgat
     4381 cacttacgtg tataaaatta aattcacttt caaaatctaa aaactaaatc caatcatcta
     4441 ccctatgaat tatatcttga aattcattca taaatagtga agcatggtaa ccatcacata
     4501 cagaatgatg aagttgcaga gcaactggta tataaatttt attattctca ctataaaatt
     4561 tacctatcgt aataataggc aataaaaagc tgctattgtt accaatattt aaattaaatg
     4621 aactaaaatc aatccaagga atcattgaaa tcggtatggt gtttt
//
LOCUS       DAZKMZ010000128         4659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000128 DAZKMZ010000000
VERSION     DAZKMZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.132"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..877
                     /locus_tag="ACG8M5_002603"
     CDS             <1..877
                     /locus_tag="ACG8M5_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0359794.1"
                     /translation="WLVLDSAKPEEIEQAIEEFELPDDIFVGTEYPEEVSRMERLYET
                     KLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDRKTEFVDRLLTKHGNHL
                     NSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTENEELYKQADLERDIVYI
                     DHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEKMIEIYRDLLESIGDLF
                     NGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGKSSALGFILVVFGSLIA
                     AVVLGYHLYRKDYTN"
     gene            955..1848
                     /locus_tag="ACG8M5_002604"
     CDS             955..1848
                     /locus_tag="ACG8M5_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0359795.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            1886..2449
                     /gene="amaP"
                     /locus_tag="ACG8M5_002605"
     CDS             1886..2449
                     /gene="amaP"
                     /locus_tag="ACG8M5_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0359796.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            2463..2651
                     /locus_tag="ACG8M5_002606"
     CDS             2463..2651
                     /locus_tag="ACG8M5_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0359797.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            2664..3566
                     /locus_tag="ACG8M5_002607"
     CDS             2664..3566
                     /locus_tag="ACG8M5_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0359798.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            3598..3840
                     /locus_tag="ACG8M5_002608"
     CDS             3598..3840
                     /locus_tag="ACG8M5_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0359799.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(4096..4467)
                     /locus_tag="ACG8M5_002609"
     CDS             complement(4096..4467)
                     /locus_tag="ACG8M5_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359800.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         1680 a          809 c          939 g         1231 t
ORIGIN      
        1 ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga
       61 gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg
      121 cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc
      181 agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt
      241 gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa
      301 tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta
      361 cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa
      421 aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat
      481 cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt
      541 catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac
      601 agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg
      661 gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat
      721 ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa
      781 aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt
      841 aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc
      901 aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca
      961 gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat
     1021 caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc
     1081 tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg
     1141 atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc
     1201 ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt tgcaggacgt
     1261 aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat
     1321 aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt
     1381 tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac
     1441 atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca
     1501 atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca
     1561 gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa
     1621 ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa
     1681 ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt
     1741 cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt
     1801 acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt
     1861 ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg
     1921 aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt
     1981 gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata
     2041 tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct
     2101 gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt
     2161 ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca
     2221 ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga
     2281 gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa
     2341 tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg
     2401 ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa
     2461 aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta cttggattga
     2521 tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg
     2581 cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt
     2641 ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga
     2701 acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg
     2761 tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta
     2821 acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc
     2881 caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg
     2941 tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc
     3001 acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt
     3061 ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa
     3121 tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct
     3181 ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag
     3241 tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg
     3301 aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt
     3361 tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa
     3421 aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg
     3481 cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag
     3541 aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg
     3601 ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg
     3661 aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca
     3721 cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca
     3781 tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa
     3841 aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca
     3901 aacaaaaata gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt
     3961 ttgaacactg tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt
     4021 tatgcgggca aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga
     4081 tacagagaag cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt
     4141 gaacatacaa aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg
     4201 atactgatga agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa
     4261 gaaataatat ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc
     4321 tagacaaaca aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc
     4381 tgctagacga ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga
     4441 aagaaaaaag taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca
     4501 ttttaaaata acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat
     4561 gtatttttaa ataaataatc attattatca aaataatgtt aaaaataaaa aagaattgtc
     4621 catcactgtt attcaaatca ttttatcaaa actacatag
//
LOCUS       DAZKMZ010000129         4620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000129 DAZKMZ010000000
VERSION     DAZKMZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.133"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1192
                     /locus_tag="ACG8M5_002610"
     CDS             152..1192
                     /locus_tag="ACG8M5_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0359801.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2016..2348
                     /gene="traE"
                     /locus_tag="ACG8M5_002611"
     CDS             2016..2348
                     /gene="traE"
                     /locus_tag="ACG8M5_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0359802.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2967..3308
                     /locus_tag="ACG8M5_002612"
     CDS             2967..3308
                     /locus_tag="ACG8M5_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0359803.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(3545..>4620)
                     /locus_tag="ACG8M5_002613"
     CDS             complement(3545..>4620)
                     /locus_tag="ACG8M5_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0359804.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1603 a          672 c          884 g         1461 t
ORIGIN      
        1 gtagtacata attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta
       61 atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta attttacata
      121 aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga
      181 tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata
      241 caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat
      301 cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa
      361 tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt
      421 ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa
      481 taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact
      541 cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat
      601 acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat
      661 cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa
      721 taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca
      781 attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc
      841 tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac
      901 cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtgata tttcgatagt
      961 ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt
     1021 aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt
     1081 ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg
     1141 tttgaattat gcaagctccc caagcactat tacgaaagac tggttagagt gaaactttac
     1201 tgacaaagtg attgttgctt catggagtaa cattaaaatt aaaaattttt tattattcta
     1261 attatattac gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat
     1321 tatgataaaa atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt
     1381 tcaaatatag aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac
     1441 gcgaagagag ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca
     1501 ggcaatacga atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg
     1561 tgatgaattg attttcgttt tgcacttttg gatgataaat ttggacaatt gaaaatacaa
     1621 aaaaaagaaa ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg
     1681 cattaagatt tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga
     1741 tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa
     1801 tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct
     1861 ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt
     1921 ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg
     1981 ttaaaaatat ttaaagaaat tgggtggttt attttatgtc atatacagga attttgagtc
     2041 tcaaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac
     2101 tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca
     2161 aagtctcaga ggaaatgatt tattacattg ggaaacaaaa gcaaataatt ttaaaagatc
     2221 ctatcccctt aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac
     2281 tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga
     2341 tcggctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata
     2401 ttgtcgagac ttctaaagga acttagagtc actagaaaaa taaggagtag acttgatcta
     2461 tcaacaattc ccttcgggga gtgacggggt aggttttaac gattggtaac gtaaaaaatt
     2521 ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa tgaatttgtg taaataataa
     2581 gcgcagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg tctagattaa
     2641 tcatgagcag ctactaaggg atctagactt tgacgcaaac tcatggaata aggagtaggt
     2701 cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata
     2761 cttgcgagga cgttgattat tggatcaatc gagcatttac cgctgtctac tagggtttct
     2821 cgtttcggac gccataaacg agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg
     2881 ataaaatatg aacaagtcct gaataaagtg aaaaaattaa tataaagcat agagaaaatg
     2941 aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata cgattgggca
     3001 cacatactat taaggtttct ttccaacgtt gggattacaa aggatttgtg acgtttagaa
     3061 ggggcggaaa ttgtaaagga ttagatgttt tagcattaga cgaggatgat ttatacgatc
     3121 aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat gaaggtaatg
     3181 aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt gaagatactt
     3241 ggtcatattt gagtgattat attgtcagct ttgaaatcat tgaatttgta gcggataaag
     3301 aagaataaaa actggaggaa taaattttac agataaaggc ttagaaggag tcacgacgat
     3361 taataaaaag gtagtattac cttaggcatt gagtaaggaa tttatttagt ctaatggtga
     3421 taagtcaaga tgaaatttaa gattttcttt attgagtaat atgttttgaa gcgcacgaaa
     3481 ctagacccga tcaaaatcaa atttttttcg ggttgattca aggatttgcg tcaataacgg
     3541 attgttattt ctctatcaca ctccttggtg gcgtatagag ttggtgttta cgtagtagcg
     3601 tatccaccag acgcgtaaat tttcttgcgg ttaggacgag tgcacgtttg tgttgatgct
     3661 ttggaacctc cttatattta ctttgataaa aagctttgta ttcaggaaga taatttttta
     3721 cagagttggc agcttcaact aagtagtatc taaaatatcg atttccttgt tttgtcagag
     3781 gtgtattttg cgactgatag tttccagatt ggttcacttt ccaacttaat ccagcgtatt
     3841 ttgccaattt tgtctgatct tcaaatcttt cgatttgacc aatttcagcg attaaaccgg
     3901 cagcatagac tttaccaacg cctggaatac tggttaagca ttgatattct ggaatgacaa
     3961 caattacttg ttcgatggct ttatctaatt ctttgatatt tttttcaagt gctcgtattt
     4021 ctcgtacgag aacacttaaa acaacattaa ttgattcttg tgcaagagca cctaggcggt
     4081 aactcatggt aattgcacgt tgaattgctt tagcgatttt ttctggttgc ttgaaacggc
     4141 cttttccttt ttcttgaagt aaatcacaga aagcttctaa aggaagctca gcaagttcat
     4201 ccaatgtgaa atcttccgtc atgagtgaaa tgagggttgc actaaaaaga ctcgtcgatt
     4261 cgtctcgcat ttctcgagca agcgtattac atttgtacgt tagattttca agaaaatgtt
     4321 gctttgtacg aatcaattgc ttaataagtt gatagcgagt acgtgttaaa cgctgtaagg
     4381 ccatgtattt ctcttctttg ataggggaag ttgtgaatcg ttgaatacgc aagaaatcag
     4441 cgattcttaa tgcatcgttt cgatcagttt tattctcatc aaacatgcga ctaaattgtt
     4501 tcacccgaaa aggattttca atcgttacaa gtgtgttgag cttctttagt ttctcatctt
     4561 catgaaaaaa catggaagga tggaagctgt acatggacgt tgactccatg ccaatcacaa
//
LOCUS       DAZKMZ010000130         4542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000130 DAZKMZ010000000
VERSION     DAZKMZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.135"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..530)
                     /locus_tag="ACG8M5_002614"
     CDS             complement(9..530)
                     /locus_tag="ACG8M5_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0359805.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8M5_002615"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8M5_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0359806.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
BASE COUNT         1458 a          839 c          992 g         1253 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca ta
//
LOCUS       DAZKMZ010000131         4484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000131 DAZKMZ010000000
VERSION     DAZKMZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.136"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M5_002616"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M5_002616"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0359807.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8M5_002617"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8M5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0359808.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8M5_002618"
     CDS             2067..2819
                     /locus_tag="ACG8M5_002618"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0359809.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACG8M5_002619"
     CDS             3683..4216
                     /locus_tag="ACG8M5_002619"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0359810.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1488 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DAZKMZ010000132         4434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000132 DAZKMZ010000000
VERSION     DAZKMZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.137"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8M5_002620"
     CDS             complement(543..2048)
                     /locus_tag="ACG8M5_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0359811.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8M5_002621"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8M5_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0359812.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8M5_002622"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8M5_002622"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0359813.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKMZ010000133         4385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000133 DAZKMZ010000000
VERSION     DAZKMZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.138"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..209
                     /locus_tag="ACG8M5_002623"
     CDS             <1..209
                     /locus_tag="ACG8M5_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIB"
                     /protein_id="HFQ0359814.1"
                     /translation="EELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKN
                     IYFQATPSAPKTGLKDVLNKFDLS"
     gene            246..647
                     /locus_tag="ACG8M5_002624"
     CDS             246..647
                     /locus_tag="ACG8M5_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359815.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            759..1112
                     /locus_tag="ACG8M5_002625"
     CDS             759..1112
                     /locus_tag="ACG8M5_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359816.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            1146..1589
                     /locus_tag="ACG8M5_002626"
     CDS             1146..1589
                     /locus_tag="ACG8M5_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359817.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            1606..2691
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002627"
     CDS             1606..2691
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002627"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0359818.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            2684..3397
                     /locus_tag="ACG8M5_002628"
     CDS             2684..3397
                     /locus_tag="ACG8M5_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0359819.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(3604..3753)
                     /locus_tag="ACG8M5_002629"
     CDS             complement(3604..3753)
                     /locus_tag="ACG8M5_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359820.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            4149..4349
                     /locus_tag="ACG8M5_002630"
     CDS             4149..4349
                     /locus_tag="ACG8M5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0359821.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1544 a          665 c          936 g         1240 t
ORIGIN      
        1 tggaagaatt gaatattgga gggattgctc aaaaaaatcc tgatacagat cttttagtga
       61 cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt gaaagaaatg aatgaacaat
      121 atggcgtaaa gaatatttat tttcaagcca ctccatctgc acctaaaaca ggattaaaag
      181 atgtattgaa caagtttgat ttgtcataaa caaatgctta gaaaaaagat aaagagggcg
      241 aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt tcgttatcgc tatatttacc
      301 gtaagcgccg tacaaaaaag agtaaacagc gttttctttc agcattagta tcagatattt
      361 acagtatgag aacagatgtg acagtcattg cttacgatac gctagcctac cgatcaaaaa
      421 atatttatgt tggagatatc gaaaaagcag agaaagttat ctgtacttat tacgatacac
      481 ccgttcatgc attaggaagt tatttcatgt ttgattggaa agaccaaagg aaaaaaacaa
      541 tctactctat attattgtcc ttcattttgt tgttcagttt aggttggtgg ggaatgatga
      601 tatataacaa aaatcctcat cacgtattcg atctgctatc cgtataaaca ggtatcactg
      661 tactagcatt tgggagctac tttttcttat tgggaaaagc agctcgcgga tggcctagca
      721 gacaaacttt tattcgaaat acttcatcca tattaacgat gctagaaatg atccgtacga
      781 ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg ctgttatgga gaaaagggct
      841 tagacagcgt gagatcgggt atgaaaaaag aggctattct tttttatcta gactctgttg
      901 gggcagatac accgctacag ttttcgggaa attatttttc aaataaagaa cagtggctga
      961 aacaagttga taagttgaaa gaaaaaaacg tcaattatat ttttagtgca agaaaaaagc
     1021 aagcgcagtt cttctatttg accaaaacag atttacgagg gaaaacattt aattggcaaa
     1081 atgccaatca gattattgct ctttttcgat aagtaaaaag tgcaaaacaa ggagtgagaa
     1141 gactaatgtt ggaaatcatg atcgttacac atggtaagtt gagcgaagga ctgaaagatg
     1201 cggcggaagt aattgtcggg acatcgagcc atattgaaac aatgtgctta gagtctgggg
     1261 aggacatcca aacattcgga gagaaggtca aagaaaaatt aactatgatc aacacagcag
     1321 aacccatgct agtatttgtt gatctaatta gcgcaagtcc gtacaatcaa gcgctcttgg
     1381 cagtcaatga actgactgaa gaaaagaaag cagccactta tgtcataggg ggcgtgaatc
     1441 tgccaatgtt gttagaagca atcaatcatc agatgcttca cacaccgata gaagaagcgg
     1501 tggaacaaat tgtcaaacaa ggaaaagata atttaactgt ttggcatgct tcagcggaag
     1561 aaaaaaaatt agatgaggac gatttttaga aggaaggctg aaaagatgaa gtggggattt
     1621 agatggtatg gtgaaaaagg ggattcgatc ccattaaaat atgttcgcca aataccggga
     1681 ataaatggga taattggaac tttgctgact aaaatgcctg gagatgtttg ggagatttca
     1741 gaaattgagg agttgaaagc atctgttgag agccaaggac tggaactgct tggaattgaa
     1801 agtgtagcga tccacgatgc aatcaaggca gggacagatg aacgagatta ctatattgat
     1861 aactatatac gaacaatcaa aaacttgtct cgatgtgggg ttcgattgat ttgttatagc
     1921 ttcaaaccaa tttttggatg ggcgaaaact gatttgttta atgaaaacaa cgatggaagc
     1981 tattctcttt tatacgatca gaaaatcgtc gatcagatgg agccaggaga aatgtatcaa
     2041 ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt gggaagaaga acggttgaaa
     2101 aaattcgctc agctggaaaa actgtatgaa ggaatgaccg aagaagaatt atttgaaaat
     2161 ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa aagcagatgt aaaaatggct
     2221 atccatccag atgatccgcc atgggaactt tttggttttc ctagagtgac gaaaaatctt
     2281 ggcgacttga agaaaattct aaatgcagta gattctccat ataatggact aacgctgtgc
     2341 acaggatctt taggggcaaa tccggaaaac gatttagttg aaatcatcca tgaggtaggt
     2401 tcgcgcatcc attttgtcca cttcagaaac gtggagtttt taggagaaag aaagttcaaa
     2461 gaaacggcac atccctctac agaaggctcc cttgatatgt atcatattat gaaagcgttg
     2521 gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg gtagaaccgt gtggggtgaa
     2581 gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg gactcactta catgcaaggt
     2641 ctatatgagg cgataagtaa ggaaaaacca ctgggagagc attatgaata aaatcaagct
     2701 cttcatcttt gatatggatg gccttttatt agaaactgga cgtttagctt atcgtgctta
     2761 tgtaaaatca gcacaaaagt atgattatga aatgcgtaag gaagtctatt atttactaac
     2821 tggccaaaca gaaatggcga taagagaaca aatgggcatt ttgtacggag aagatgttcc
     2881 atatatcaaa tggcgagaag caatcaatca atataaagaa aaaatcgtga aagaagataa
     2941 gagagtatat acgaaaaaag gagcagaaga aattttatct tttgcaaaag aaagaggaat
     3001 ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta gaaatgtatt taaaaatgga
     3061 aaatctatat gccttgtttg atcaaatcat ttcaggggat gatgtaaaaa aaggaaagcc
     3121 ggaaccagag atctttttga aagcttgttc aaaaatgaat attcctccaa gtgaagcact
     3181 tgtatttgaa gactcgattg cagggatcga agcagcaagg cgagcgggca ttttgtcttt
     3241 tctcatcaaa gatcatttga tagggttgcc cgatcataat gggaagcata agttgaaagt
     3301 agatgtaagt ttatatgaat ccaataaagc agactatgaa ttttatgacc tgcatcaagc
     3361 aaaacgattt ttagaaagta aagggttgta tctctaataa agaggactat tttctagtcc
     3421 tctttattta ttgtcctgca cgactgaggg aacagatgaa aaagaagtaa ttttttattt
     3481 tgaagtatct accttatttg tagtgaacaa tttagaacaa acacaaaacg catctactgt
     3541 tatctaccag tagatgcgtt taaagagaag aacgagaatc gtccatatga gggtttccac
     3601 tccttatgca gattttttta attcttgcac tgcttgttcg atcgtaacac ctgtcgctac
     3661 atgattggta ttttctttgt caaaaacgac aaatagattc aaatctgtat ccaaggttac
     3721 tcgatattgt atatctttgt tatagaatgt catagccatt ctcctttctt taaatacttg
     3781 ctgaaaggca acgaacgcgt tgataacgaa attccattta taagtatacc accaattgaa
     3841 aggttttcat agttaagaaa ttttaaagtc aaaagttgca gataaaagca aataaacaag
     3901 aaaacaaaaa gtgaatcaat cgcggttatt caactttttc ttcgattcga agacaataga
     3961 agacaatgaa atttatattt cattgcatta tccttgtgtt attagaaaaa gcatgatata
     4021 ttaaatgagt aatctatttt gaaacgtaat ttgagcgata tatctacata gataaaactc
     4081 cttttgccta tcaaatattt attgcaacga aaaaacgtac attgatacgt atcaggagga
     4141 atttatttat gaataacggt acagtaaaat ggttcaacgc agacaaaggt tttggcttta
     4201 tcactggtga agatggaaat gatgtatttg cacatttttc agctatccaa ggtgacggct
     4261 tcaaaacttt agaagaaggc caagcagtgt cttacgatgt tgaagatggt caacgtggcc
     4321 ctcaagcaac aaacattgtt aaagcttaat aaaaattgac ttttaaccgt cttttcgaaa
     4381 gaaaa
//
LOCUS       DAZKMZ010000134         4343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000134 DAZKMZ010000000
VERSION     DAZKMZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.139"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..580
                     /locus_tag="ACG8M5_002631"
     CDS             143..580
                     /locus_tag="ACG8M5_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359822.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            675..1559
                     /locus_tag="ACG8M5_002632"
     CDS             675..1559
                     /locus_tag="ACG8M5_002632"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0359823.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1781..>4343
                     /locus_tag="ACG8M5_002633"
     CDS             1781..>4343
                     /locus_tag="ACG8M5_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0359824.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1419 a          726 c          967 g         1231 t
ORIGIN      
        1 caacagtagt ttaattatta cttgatttag ttctttttat tttaaaaatt ttttttactt
       61 agaatccagc caccctagat atttttctgt ataatcttaa ataaagaaaa cgatattaaa
      121 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
      181 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
      241 tttgtatata aaatatattc atcaattttc cgttttggta ttaggcacac taattatagc
      301 ggggattgtt gcatttatta ttcaaggtcg tgacatatta acagtacatc aagagttttt
      361 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
      421 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
      481 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
      541 cagtttcata gtatgggtat tttatcttgt caaaaaataa atgaatgttt catgaacagt
      601 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
      661 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
      721 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
      781 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
      841 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
      901 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
      961 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
     1021 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
     1081 tcgcaattgg ctctatccct gaagcaatca atgatgtaga tgcggagaag aagacgaaat
     1141 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
     1201 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtatc ttgctgatag
     1261 gactaactaa ctcagtaggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
     1321 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
     1381 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctatctt acttgtgagc
     1441 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
     1501 gggataaatt acctgtgaca atcaaagtgt taccctcaca tttacaggtt tattgttaaa
     1561 atggaggttg tgaccgaaga gttgtcagga ggacatcggt tgttcggaat aagggactgt
     1621 gacaagactt ttgtcacagc ctttttttgt acaaaaaaga aattttcacc taacagaaaa
     1681 atttgccata aaaatacata ttttaaatat gaaagtacaa ttacaaataa attaaaacgt
     1741 taaaataaaa ttgtcaaaac gtcagagagg atggtggaga atgaatcaat atgtaaaaaa
     1801 gctgattgcg tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga
     1861 tgtagagaaa acaagcgaat caacgcaatc agagaatcaa agttttgaca agactacttt
     1921 ttcagaagaa tcaattactt ctgaaactac agaatcccaa agtgaggcgc ctacttctaa
     1981 tacaaggcaa gaagaaaata agacaagtga gacctcagaa gtagctgaac aaacgtctga
     2041 tagcagtgaa gaggtacagg ttagagttgt ttcagcggat gggtacagcg aagctgccac
     2101 aacagaagaa ctgacgcagc ttctcgcaga cgaatcagta gctaagattc gtttaactca
     2161 gcctttgact ttagacaggg aattggagat caagcgagat atcgttatcg attttggcgg
     2221 acttgctcat aatttcggca cacatcacat ctatatcaat gagacacctc agtcgatcga
     2281 gtttcagaat tttaaaggaa cggcagctca tccaggtggg ttgataccaa atatcgatgg
     2341 aaatgccatt atcattgctt atatgtccga tttttgggga aacagatatc attttacggg
     2401 agaaataaaa tttaccggga cacttgactt atatgatggt tctaaattag gcctgattta
     2461 cgcccctcgt gcaactgtca cattagatgg tgtttccggt gtattggatg tccaaccagt
     2521 gaaagaaggg atgaatgccg ctccgggtaa agcttatttt tgtagaagct atcagctaaa
     2581 tgtaatcaat ggtagtcagc tttatggacc atatctagga aaattctatg gttttctaga
     2641 ttcaggtgat gagacgggca gtggaaaaac agcaccagga ctccatatct tgagtggttc
     2701 aaaggtctct cttgactatg atcgggcaga tggcgtcagc gatgaaggag aggctgtcga
     2761 tacgctaccc gggaatgtca catttaaagt cagtggctca ggaagtgaat tttcggtaaa
     2821 tacaaagatc aatattacca acgacaacaa tcgcggaatt atccaaatga gaggctcagg
     2881 atcaagagtc gctgtgtcga atgatgggaa aatcacaatc aatacagcga ctactggcgg
     2941 atttcgcctg caaggagatt ctagtcaaat ccatgtatca agtggtggac aaatctcggt
     3001 caaaatggct ggagatggcg accaacctgg aaacaatggg atgcgttttg tagggaaagg
     3061 actttctttg atcgtagaag gagccggatc agtcatcgat attgataagc aaagtggtcg
     3121 ttctagcgca gttcgttttg agaacggtac gcaaaaacta acagtaacta atggaggctc
     3181 attgaaggta agaaatgcag gggatggcaa atcttatgtg aacagactca atcaaggaaa
     3241 ccaagcgatc caattttatg acgcttctgg ttttctagaa agtccagggt cagctgattt
     3301 tacggttact ggagaaaaat caaatatcga tatacgcgca gattccggag ctaccgtaga
     3361 tacaactgga gatattgatt tgaactttat ggctggcgaa aaaacttatt tagtcatggt
     3421 cgggaaaact ggggaagata agactggcat catcagtggg gatgtgttga atgtagactt
     3481 agaatctcct acctatttcg atttccaaaa caaacggatc ggaagcgaga ctaatggcat
     3541 aggtggctgg gtatttcaag gaaatggcga ttcttctttg aagatcgctc attcagaaat
     3601 tgctttatgg cgaaaggcag gagtaacgtt cgataacttt gaaaaagatc cggattattt
     3661 ttctgatatg acagacttat cgatcaatgg aaccgactta ggtaattttg tatcaagccc
     3721 tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc ggaatgaaaa attataatcg
     3781 aatcagtgcc aataaccagc ttccgatgat tgaatcctta agagtaccaa cgaatgcaga
     3841 taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc
     3901 ttggacaggc gaagtagaag tgactttacg tattacttat gcagatacga cgaaatcacc
     3961 tgtagaaatt actgctctca cacaaggaca aacagatgaa aatgaaggga caggatataa
     4021 tccgtgggga gaaggcgaac aaggcggttt gtttgaggtt caagtaccca acgaagaaag
     4081 actaacaacg ggggatcaag tgcaaatagt tgctgctaaa aaagtaagag gcggccaaga
     4141 agtgacagaa ctggactctg ccaaaacgac agtcgatgtg attcctcctg agcctgctaa
     4201 attaagtacc acattgatca atgcagcttc tcgctcaatc acaggtacag gcgaagcagg
     4261 agctttggtt tcattgaaaa aaaatcaagc tgttttgtcc gaaacaacta ttgatgatac
     4321 aggtaaattt gttctttcta tcc
//
LOCUS       DAZKMZ010000135         4336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000135 DAZKMZ010000000
VERSION     DAZKMZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.140"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8M5_002634"
     CDS             <1..288
                     /locus_tag="ACG8M5_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359825.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG8M5_002635"
     CDS             347..787
                     /locus_tag="ACG8M5_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359826.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG8M5_002636"
     CDS             799..1467
                     /locus_tag="ACG8M5_002636"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0359827.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG8M5_002637"
     CDS             1531..2154
                     /locus_tag="ACG8M5_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0359828.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG8M5_002638"
     CDS             2210..2596
                     /locus_tag="ACG8M5_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0359829.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG8M5_002639"
     CDS             2809..4200
                     /locus_tag="ACG8M5_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0359830.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1442 a          723 c          925 g         1246 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttg
//
LOCUS       DAZKMZ010000136         4297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000136 DAZKMZ010000000
VERSION     DAZKMZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.141"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8M5_002640"
     CDS             <1..792
                     /locus_tag="ACG8M5_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0359831.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8M5_002641"
     CDS             889..3138
                     /locus_tag="ACG8M5_002641"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0359832.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4297
                     /locus_tag="ACG8M5_002642"
     CDS             3141..>4297
                     /locus_tag="ACG8M5_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0359833.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1395 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagt
//
LOCUS       DAZKMZ010000137         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000137 DAZKMZ010000000
VERSION     DAZKMZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.142"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8M5_002643"
     CDS             complement(37..1257)
                     /locus_tag="ACG8M5_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0359834.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8M5_002644"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8M5_002644"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0359835.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8M5_002645"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8M5_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0359836.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8M5_002646"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8M5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0359837.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKMZ010000138         4260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000138 DAZKMZ010000000
VERSION     DAZKMZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.143"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(381..878)
                     /locus_tag="ACG8M5_002647"
     CDS             complement(381..878)
                     /locus_tag="ACG8M5_002647"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0359838.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(918..1727)
                     /locus_tag="ACG8M5_002648"
     CDS             complement(918..1727)
                     /locus_tag="ACG8M5_002648"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0359839.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2061..2918
                     /locus_tag="ACG8M5_002649"
     CDS             2061..2918
                     /locus_tag="ACG8M5_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0359840.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2911..3813
                     /locus_tag="ACG8M5_002650"
     CDS             2911..3813
                     /locus_tag="ACG8M5_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0359841.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3846..4196
                     /locus_tag="ACG8M5_002651"
     CDS             3846..4196
                     /locus_tag="ACG8M5_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0359842.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
BASE COUNT         1427 a          734 c          781 g         1318 t
ORIGIN      
        1 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct
       61 tatgttttta cttggctgtt cttattactg cagatgcaag cttttgaata ccgtttaagg
      121 aagaggttgt gaaatcagcg ttttgacctg agtattagct taacaaacgg aaaaatagtt
      181 cctcatattt tgccgtttgt taagctaata ccgagggtca gctgattgaa caccatttaa
      241 gaaagaggtc gtgagcctgc cgtttcactc tgtgtcataa aaaagccaat ctactttgat
      301 agaaaaagca cgtaggacta aaacagttct gcgtgctttt tttattgtgt gttaaatatc
      361 gtaaatgaag atagatattt ttatgattcg acgatagaga cgttgaaatc tttcaacagc
      421 tctaaagcgg attctccgtg ttttctactg gagactaaca ttggatcaac aaaaaagtct
      481 gcttcaggaa aagcactctg caggcctatc agattagaga gtacacacaa gtttgtttca
      541 atcccgacta cttcgatctc tttttgttct tctttgcttt caaataaagt ttcctggatt
      601 tcagctaaag tttcaggtgg aatcgtaaag taatactttt taatcactcg ttcattttct
      661 tgtggagaaa gaagagggat caattgcaaa tcttctcgtt cttcttcttt gtccttcagt
      721 tcaactggta tatcccgtgt gaagagaata tattcattat tttggcgtgc tttagctaat
      781 ctttgagcaa tacgttctgc caattgatct gactctggaa tataaaattc gctgtttgga
      841 tcaagaatat gattttgcat atcaataata actaacattt tctttcctcc tacattcata
      901 tcgtcatttt attcttttta atagaagttt tttaaaatat aagcaacacc agcttcttca
      961 ttcgtcagtg ttacttggtt tgcaatttct tttacttgtc gctgtgcatt ccccatcgca
     1021 atactcaacc cgctgtaatt caacatagca atgtcgtttt cttgatcgcc aaaagccatg
     1081 atgtcactcg attcgattcc aaggtaatcc gccagaaaaa caagtgaatg agctttagtg
     1141 atacctgtag gcgtgatttc taagaaatgc ggatcagaga atactgattg gataccatct
     1201 gcttgcagat ctctttgtag cttctgtagt ttttcttcac tcttatctgt taatgctaat
     1261 ttaagatagc taagagaagg atcttccaac atatttaaaa tttgatcaaa agaatggttc
     1321 tgtaaattga aatgttcata ataagggtca tctttgacta gatttgattc atcgaatgtc
     1381 agctgatcat cttgggtttc tcggctagca atgatggata ttttcgcttc ttgagcatag
     1441 aagaaggctt ttctcagtgt atcgctatct atttttgaac gtctgagaac tttcgccatt
     1501 tttccatcct tcatttcagc aatcagtgaa ccattggaag aaatcaaata gccaccgatt
     1561 cctaattcgg aaaaaatatc tttagcagaa atgctgttcc ttccggtagc gacggttacg
     1621 attccgccat tttcatcaaa tttccgaagt gcttggatat tttcttctgg tatctttcca
     1681 tttcgatcaa gcaaggtacc gtctaaatca atcgctgcta gtttcatttt atcttcctac
     1741 tttcttttta catatctatt ttcctgttac cttaagatta aatgagaatt taaaaaaatg
     1801 aaactaactt gctttgtcat tatgaatttt ttcaaaaaac attcttaaga aaaaggtggc
     1861 tatatatagt agtttttggt caataccttt gaataaaaag ccatgaaaaa cgaatagtta
     1921 gcgcttaccg atgcgttttg tgataaaaaa attttaaaat attcaatgaa aacaaagcat
     1981 atattttgct ttaaaaccaa atgaggaata ttctagatgt agaacaggtt agaaaaagat
     2041 tagaaaaagg agtgattttg atggagaaga gatacggcgt agttcctgtg gtaactgctt
     2101 cagacgaaaa ttatgctcct tacttgagcg tgatgatcgc aactgcatta gaaaattgca
     2161 ataaaacaag acgcataaaa ttttatgtga ttgacgatgg tctatcagaa tatagtaaag
     2221 aaggattgga agaaacggta aataaatatt caagtaatgc aagtatccaa ttcctgacag
     2281 tggaaaaaga tatctacgaa gacttcctag tcagtgacca tatcacaact accgcttact
     2341 taagaatttc tttaccaaac ttattagcta aagaggatta taagaaagtc ctttacttag
     2401 attcagatgt gttagtacta gacgatatcg taaaactata tgatgagccg ttaaatggaa
     2461 aaacaatcgg cgctattatc gatccaggcc aagtgaaagc tttggaaaga ttaggaatcg
     2521 attccgatga cttgtacttc aattcaggag tgatggtgat tgacattgat caatggaata
     2581 aaaaagaaat tacagaaaaa actatccatt acctcagtga aaatggcgat cgaatcatct
     2641 atcacgacca agatgctttg aatgctgtac tttatgaaga ttgggaacaa cttcatccaa
     2701 aatggaacat gcagacttcg ctgatttttg aacgtcatcc agcaccaaat gaaaaatatg
     2761 aacgtcagta taaagaagga aatgaaaaac cttcgatcgt tcactttaca ggacatgata
     2821 aaccttggaa tacgttgaaa gatcatccgt ataccaattt atacttaaaa aaattagcac
     2881 acagtactct aacgaaagta ggcgagatca atgaataaaa aagaaatagc agtagtcgca
     2941 agttgtaaca caaaattcgt accacattta gctgcattat ttgtctcagt attagataat
     3001 tgcaacccgt ctaaatttgt tcggttctat gtgattgacg atgatatcga ttttgaaagt
     3061 aaacagcttt tacgcttctc agtgaaaaac gcacgaatga actcagatgt ggaattcttg
     3121 aaaatcaata aagaattctt tacaaatgtg gtaatcagcg atcgtattcc agaaacagct
     3181 tattatcgta ttgcgattcc ggaattgttc cgcggcacag aagttgaacg tatcttatat
     3241 atggattgcg acatgatcgc tttacaagat atctctaaat tatggcgtct tgacttcggt
     3301 gattcaatcg tagcagctgt tgaagacgct gggttccatc aacgactgga aaaaatgaaa
     3361 atttcagcta aatcaatgcg ttatttcaac tcaggtttga tgctgatcaa tgtgaaaaaa
     3421 tggttagatg aaaatattac gcaaaaagta ttagatttca tcgaacataa tccagaaaaa
     3481 ttacgattcc atgaccaaga tgcattaaat gctatcttac atgaccgttg gttaccactt
     3541 catccgcgtt ggaatgcaca aggctatatc atggcaaaag caaaaaaaca tccaacagca
     3601 gcaggtgaac gtgaatacga agaaactcgc aacaatcctt acattatcca tttttcagga
     3661 catgtgaaac cgtggagcaa ggattttgaa gggccaacga agaaatatta cgaaaaatat
     3721 gcagggatga cggctttccg ttgtgtagct aaattcccta aatatccaaa atatgcaaaa
     3781 gttcaaccaa aaacagatgt agttgttaaa taatagaaga caacaactga agagggggag
     3841 taggaatgga aattacaatg tctaaaaatg cagtagagac gttaattgaa aaggttgggg
     3901 aaaatacaaa aatcgcactc gcgcttatca atgattccga tccatttcta agggacaaag
     3961 gtgcttttgc aaaaggcagc ttttttcaaa tcattccttt tgtttcggaa tttggagaat
     4021 atgcgactaa aatcgagcat ccattattgg acatctatac atctaaatta gagcaaaatt
     4081 atttcggtaa acgattgaac atggacttca acaaacagct tgacagcttt tcattagaaa
     4141 atgaaatcgc agtactcgat tataatatca aattaaagaa ctgcttcttc agctaatagt
     4201 ttcgagaaag tccaaaaagg aggtatacat tgagatacta ctttgtggat gaagaaaaga
//
LOCUS       DAZKMZ010000139         4208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000139 DAZKMZ010000000
VERSION     DAZKMZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.144"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..233)
                     /locus_tag="ACG8M5_002652"
     CDS             complement(<1..233)
                     /locus_tag="ACG8M5_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359843.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALS"
     gene            complement(441..1367)
                     /gene="glsA"
                     /locus_tag="ACG8M5_002653"
     CDS             complement(441..1367)
                     /gene="glsA"
                     /locus_tag="ACG8M5_002653"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0359844.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1390..2817)
                     /locus_tag="ACG8M5_002654"
     CDS             complement(1390..2817)
                     /locus_tag="ACG8M5_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0359845.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2848..4062)
                     /locus_tag="ACG8M5_002655"
     CDS             complement(2848..4062)
                     /locus_tag="ACG8M5_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0359846.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1306 a          903 c          755 g         1244 t
ORIGIN      
        1 gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct
       61 tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta
      121 gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt
      181 tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat
      241 acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt
      301 tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt
      361 caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc
      421 ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct
      481 ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca
      541 cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca
      601 ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata
      661 attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca
      721 attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg
      781 gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta
      841 cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag
      901 atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt
      961 cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct
     1021 gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa
     1081 ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg
     1141 tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca
     1201 tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat
     1261 tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa
     1321 tagtggaaat tttttccaat caattgttct aattctttta cttccatttg aataaccagc
     1381 tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg
     1441 aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc
     1501 agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag
     1561 acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc
     1621 aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat
     1681 caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc
     1741 gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg
     1801 agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc
     1861 tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc
     1921 taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct
     1981 taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac
     2041 tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac
     2101 aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga
     2161 tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt
     2221 cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc
     2281 gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa
     2341 accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc
     2401 ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat
     2461 acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt
     2521 tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact
     2581 gacccaagta tagacaccgc ctttttccca tcctttcacc gttgccatct ctgctgcgca
     2641 caatgccaca gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa
     2701 gcctgaggtc gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc
     2761 caaaaagcca aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa
     2821 tcttcctctt ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc
     2881 caatccttct tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt
     2941 taaaatcact gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg
     3001 aatccctaag aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat
     3061 ccctatcaga atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc
     3121 atcatcccat tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat
     3181 gatcccaatc agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc
     3241 caatccagca acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt
     3301 tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc
     3361 attgactgct tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa
     3421 ccaaagcagt cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc
     3481 taaaggaatc tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt
     3541 cgtgtggatg actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc
     3601 accccatatc cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat
     3661 aatatagcct tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat
     3721 cacgataaac atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa
     3781 catgttgggg aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac
     3841 accgccgata tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt
     3901 tatcccaacg gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa
     3961 tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat
     4021 catgaatgct gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct
     4081 acgattacga gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata
     4141 cttgcattta taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt
     4201 tttcacac
//
LOCUS       DAZKMZ010000140         4183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000140 DAZKMZ010000000
VERSION     DAZKMZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.145"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8M5_002656"
     CDS             75..503
                     /locus_tag="ACG8M5_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0359847.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8M5_002657"
     CDS             490..732
                     /locus_tag="ACG8M5_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359848.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8M5_002658"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8M5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359849.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8M5_002659"
     CDS             1354..1668
                     /locus_tag="ACG8M5_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359850.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8M5_002660"
     CDS             1772..2950
                     /locus_tag="ACG8M5_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0359851.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG8M5_002661"
     CDS             complement(3131..3316)
                     /locus_tag="ACG8M5_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359852.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG8M5_002662"
     CDS             3353..3646
                     /locus_tag="ACG8M5_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0359853.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG8M5_002663"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG8M5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1425 a          625 c          817 g         1316 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taa
//
LOCUS       DAZKMZ010000141         4072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000141 DAZKMZ010000000
VERSION     DAZKMZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.146"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1780)
                     /locus_tag="ACG8M5_002664"
     CDS             complement(14..1780)
                     /locus_tag="ACG8M5_002664"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359854.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1740..3494)
                     /locus_tag="ACG8M5_002665"
     CDS             complement(1740..3494)
                     /locus_tag="ACG8M5_002665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0359855.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3604..>4072)
                     /locus_tag="ACG8M5_002666"
     CDS             complement(3604..>4072)
                     /locus_tag="ACG8M5_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0359856.1"
                     /translation="HSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMG
                     GAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRF
                     RQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1364 a          776 c          579 g         1353 t
ORIGIN      
        1 aaagcctatt tcatcatcgt gattgagctt ctttttcata gacgaatttc tcagatagtt
       61 tagttttctt tatgagattt ttgtatattt ctgaccttcg atacaactca ttatgagtac
      121 cttgatcaac tagttttcct tgatccatca ctacaatttt atttaccttt tcaatagact
      181 tcaagcgatg cgatataatt agtactgtct tatcttgtat taaacgattt aaactttcct
      241 gaatacgttt ttcattttcc gcatctaaac tggcagtaat ttcatctaaa attaaaattg
      301 gcgcattttt caaaaaagcc cgtgctatgg ataggcgttg tcgctctcct ccagaaagtg
      361 aacttccgtt ttctccgacc aatgtatgat aaccgtcagg tagtttttca ataaagtctt
      421 cgcagttagc taaacgagct gcttttttca cttcctcatc tgacgcttga ctatttccaa
      481 tacgtatatt ttccaaaata cttgtattaa acaaagttac ctcttgaaaa acaatagcga
      541 ttttgctaaa caaagaagct ggagatacac gtttaatatc atacccatca attaaaatac
      601 agccttcatc ataatcatac agtctagaca ctaatttcaa gatactagtt ttcccggaac
      661 cactagcacc tactaacgca gtcacttctc cttgtttagc cgtaaaagag atatgatcta
      721 aaattggggt attgttatca tatgagaaag agacatctcg caactcaatg tcaaaagatt
      781 tcagaggact atctgatccc tcttggatac tggtttcctt catagctcta attcgctgta
      841 ttttaggagc taaataaaat atttcaagca ctgcttcttt catagaatcg aaggaatcct
      901 ttatttttat tgcggctagt aagtaaccaa cgacgtagag tatagtcact tcacccgtta
      961 acagtaagtg aacacctact agaatgacta ctgctaaact aacatatgag aaaatagagg
     1021 ataaggccat gactgaaaaa gtacttaatt ctacctttaa atgaatcctt tcactctctt
     1081 ccattttttt gaaaagtctg tcttgaactt ttttagatag attaaagcta ttaatctctt
     1141 gctgcaattc tatagtttct tgaaattctt ccgagttttc tctcaaagta tcgtaatatt
     1201 tagtattggc tttcgtttgg gatttctttg ataacaagat taagacaaaa ctaaataacg
     1261 ttggcaaaat aacagctaat cccattttga cattaccaac caaaagcatc actgaaataa
     1321 aagggaaaaa cagagccata ccgccggctt taggtattgc atgactcatc gcatgctcaa
     1381 taccttcaac gtcagacatg atagtttgag ataaatctga taaattatgt tttgaaaaat
     1441 aggataatgg taaatttgac aaatcgtctg caatttgcac tcttaaatgc gcactttctt
     1501 tataagtact attatataag ttttcatact ccctatctaa caaccaatac atgaaaagca
     1561 aagtaacaaa cgatacggca aaataaagcc aacggggctt tgtattttct aaaactaatt
     1621 cgtctatcag catcattata atgaatgctg gtaccatatt aatgcagtat acaaaaaaac
     1681 tagaaataga agctttagtt aaagcttctg cgccctgatc acttaaagca aatttgtttt
     1741 tataaaaact tttcattgtc aagcctccaa tcatttgcat tctcataatt agtgaccaat
     1801 tgtttataca cttgatgttt agccattaat tcattatgat ttccacgttc tactattttc
     1861 ccttctgaca agacaagaat ttcatctaca gatttaatgc tggataatcg atgagctatc
     1921 ataataactg ttttttcttt catcagatgt tgaaaagcct tttgtagttc atactcatta
     1981 tctggatcaa tagatgcact ggcttcatcc ataataacaa tctttgcatc ttttaaaatt
     2041 gctctggcaa tagctatacg ttgtttttct cctcctgaca gataaactcc tttactacca
     2101 attaaggtat gctctctttc gggaaatttt tctaaaatcg attcgcaacc ggctaatctt
     2161 agcgcgtcaa atactttact tgctggcgct tgaggatttg ctatagaaac gttatcgtaa
     2221 atactctgat tgaatagttt cgtatcttga aagacaaaag caattgtgtt aataattgtg
     2281 tcttttgaat agtgatttaa aggcttacca ccaattttaa tctgtccctt atccacattg
     2341 tagaaacctg aaattagttt agctatcgta ctctttccgc ttcctgattt tcctactaat
     2401 gcataaattc ttttttgttc taaaactaag ttgaaatttt ctaaaacaca ctcttctttg
     2461 tatgaaaagc tgacattttc aaatgcaatg tcacaatttt taatctgtgt ctcgttccca
     2521 tattttagtt ggtcttgctt catttctaaa taaagggagt ctagtgtttc gactgcatat
     2581 cttgcttcaa acaagtgcat acttacatac attattctca tgaaagaaac aaaaataatt
     2641 ccacttaaaa aaaacaacat caacaagtca acagctaata tcttgggagt ggtaagagaa
     2701 gttataaaaa aggttatagg aactatcaaa atgcaaacta tgccaaaaaa tagccattga
     2761 aaaagaacaa aaggtgtttt cccactcaaa gaatacttat atgcaaactt agcataatcg
     2821 ttaattgctt tattcaaggc tttaaatgat gttactttag caccaaatat ctttataact
     2881 tgaattccgc ggatatattc taccgtttca gagcttaaca catctaacga ttcttgatat
     2941 ttttgaataa aatttgtatt ctctgtcatt ttctttaaca agaatacacc aattactaac
     3001 atggcgatta gaaccaatcc aattttaaag ttaatgaaaa agcctaaggc aactgataaa
     3061 acaggtgtta ctatggcttg agcactatct ggaatcaagt gagcaattgc catatgtgtt
     3121 ttagccgcgt tgtcatcaat tgtctttcga atatatcccg aagaatttaa atcaaaaaaa
     3181 cgaaaacttg cttctgttaa accgctaatt cccttttttc tcaaattagt ctctaagcga
     3241 aacccaagga tatgagaaaa taagccagaa aaaatataaa ataaagctcc taaagttaac
     3301 caagcaacag tttgaaccgc ataactctta gctaaatatt catctcctac aataattaga
     3361 gactgaagaa atttgtaaat actgtaatat ccatagactg ttaatacgac agaaaaatca
     3421 gaaaataaag tggctaacca tccgtagtat ttcattttag ggacataact aaataatttt
     3481 ttgtatactt tcattttttc ctcccccgca ttcaatttct tctcttattc gtttacaact
     3541 gttaatgctt tccgtaatag cgtaaattag agatgtacaa acagataaaa ggtaaactac
     3601 gttctatttt ttgtattttt ttaagacttt tcttccaatc aatacaccaa tcatagataa
     3661 acttgctaca actaacactg aaatgattcc ccatatacct accgcatagt ggatcattgc
     3721 atccattgtt gcagcagatt gtcctttatc tatccaatct tgacgaaaac gatctgtaaa
     3781 gaaccatata ggaatccact gtccggcagc actgcctaat tgcattagag cccaagacgc
     3841 aacttgagga attgctcgat catatccaaa tacagataga ataatatcag ctaagatcgc
     3901 catactaatc gaactgataa accaaggtaa gtacgccccg cccattaacg taaaaaataa
     3961 caataaaaca attgtgtaaa tagtaaaaat tccttttcta gggtagttat agctaatata
     4021 aacaaatatg attccagata ataatccaaa aatcccgggg gatatgctgt ga
//
LOCUS       DAZKMZ010000142         3990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000142 DAZKMZ010000000
VERSION     DAZKMZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.147"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..621)
                     /locus_tag="ACG8M5_002667"
     CDS             complement(7..621)
                     /locus_tag="ACG8M5_002667"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0359857.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(768..1451)
                     /locus_tag="ACG8M5_002668"
     CDS             complement(768..1451)
                     /locus_tag="ACG8M5_002668"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0359858.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1560..2018)
                     /locus_tag="ACG8M5_002669"
     CDS             complement(1560..2018)
                     /locus_tag="ACG8M5_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0359859.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2102..3865)
                     /locus_tag="ACG8M5_002670"
     CDS             complement(2102..3865)
                     /locus_tag="ACG8M5_002670"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0359860.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
BASE COUNT         1089 a          845 c          652 g         1404 t
ORIGIN      
        1 tcctttttat tcgggacgtg caatcgtgaa aatttctcct attgctgcat aatcatttcc
       61 tgccaaacgg cttacagcat tcaattttct tggatcgatc cttcctgtct tatcatcata
      121 gactgtttca tttaaatgat atttctgtac ttctagcaaa aataaatcag ctgttacttt
      181 atttttattc cgtatctcga catgttcata taaggatgtt tcaaaacgaa cttttgcttc
      241 tgtcaatgct ggtactgaaa tgattactga atcagttaaa gtcaagtcag tcaggtacag
      301 ttcgctttcc tcattaggca gattggctgc tgttttattt acttcttcta cgttgtcctc
      361 atcaaggata tggataacag cttctcttgt ctcaagaata ttctttgctg tgtcttttac
      421 tgcatcttgt tttctctgga tcgccaagga taagatgggt cgattcgaac tgacgatatt
      481 gaagtaactg aagggtgcga tattcactat atcttttgct gatttagtcg ttactactgc
      541 tactggtcta gggatgactg aaccaatcag caacttgtaa ttttcacgtt ctgtcaattc
      601 tgaaggactc cgttcaaaca tggtttcgcc ttctttctat aaaattcgtt aaaaagcaga
      661 tcttgaagac aaaatgaaaa gtaatgcttt tttcatcttc ttagaatctg ctttaaacta
      721 tctatcaatt tttctttttt gtcaagaatc tgtgccgtgc gtgtttttta gttatttgct
      781 ttacttgcgg cttcgacata tcttttagtg atcaattgcg gacgggtgat aatagatcca
      841 actacgactg aaaaagcacc taattcatat gctttctcta cttgttcagg agagtcagta
      901 tgtccttcca taattactgg gatatcgact tgttctatga tttgattcaa taaatcaaaa
      961 tcaggtgtct gcgtctttcg tgaatattct gtatagcctg ctaaagtcgt agacaaaaga
     1021 tcaaaaccat cttcttgcgc ttgaagcgct tcctcaaaag tcgaaatatc tgccatagct
     1081 aaccgaccgt gttcgtgaat catttggaca agttcagcag ttgttttttc tgtcggacgt
     1141 ttacgagaag ttgcatccag tgcaatgatt tctgcttgcg tctccaaaag ttcttccact
     1201 tctttttctg tcgcagtaat aaacacttct gacccatcgt aatctctttt gataatccct
     1261 attaccggta aatcaacgat tcgcttgatt tcattgatat cttctgtcgt atttgctcga
     1321 ataccaactg ctccgccttc ttttgcagcc aaagccattc tccccataat aaaagaactg
     1381 tgtagcggct cgttttccaa agcttgacaa gaaacgacga ctccgccttt gatttgttcc
     1441 agtgtatcca caatatctcc tccagtaatg ttatataaaa atacagcttc atataaaaat
     1501 gtaacccttt tctattcagg agacaataat gagctatttc tttcagcaga cctttcttac
     1561 taaaagaaaa ttacttcaaa ttcttcgtat acgaccagct tctcttctgt aaattctatg
     1621 ttcaacgctt ctttataagc atgtttaaaa aactcagcat ggactttccg ccaataagct
     1681 aatgtccgat ccccttctcc ttctttccaa gcatgctctt tcgtgacttc actaaaaggc
     1741 aagatggtca catttatatt tttgatgata caaactggtt cattttttcc attcaggacc
     1801 acgtctaatt gaccaacttt tggtaaaggc tcattctctt gtttatacag atcgtaacca
     1861 cttgatgtac cagtctttat cttttttaca actagttcgc tcaactcgtc tgccattttt
     1921 tctgtatccc caaatgaaaa gaaacctaat ttttctgatt gataactagg atatttttct
     1981 tggaactttt tgacatattc ttctacttgc tgatacataa catccacccc tttgcttttt
     2041 ataatgaaaa aaccatccag atactagtat aaacaattat ctggatggtg agcataatga
     2101 attattgaat attcaagaca cgcagactat cttcaacgat ttgttttggt gttaaatgat
     2161 aacgttcata aagctgccct acaggaacgc tatccgtgaa ttctttctct gcaccaaaat
     2221 ttaatacgtt cattgaagaa tcaccataat aacgactgat attttcgcca aatcctcctg
     2281 acaaactacc atcttctaat gtgatgacta attcgtgtgt ttcttttaat ttatctaatg
     2341 tttctacgtc attttctgtg atcgttcctg gattgatcaa cgttgcttcg attccttttt
     2401 ttctaaaagc ttttactgtt tcttttccta attcaagaaa accgcctaaa gctaaaattg
     2461 ctacttttcc tccattattt actgtttgat aggctggaat actataatca tttgcgatag
     2521 ctgtaccatg ttctacacca tgtgtaggaa ttcggattgc tacaggctgc gctgtttgtt
     2581 cgaacgccca atctagcata gagatcatat cctcttttgt tgctggagca agatatttga
     2641 tgttcgggat acttgataag taaggaatgt caaatgttcc ttggtgtgtt cggtcactag
     2701 ctgagatcgt tccgccaccg actaaaataa tcactggatt ctcgttgatc gctaaatcat
     2761 gtgataattg atcatatgcc cgttgcaaga aagtacttga ttggaagacg actggacgta
     2821 ctccctgaga agccaaagca gaagcgaaag tgatagagtg ctgttcagcg atccccacgt
     2881 caaaataatg atctggatgt ttttcttgga atgttttcag tccaaaggcg cctggtatcg
     2941 ctgcattgac tgcggcaata ggagctgtac ttgtttgcat tttcttatcc aaataatcca
     3001 tgatgatcat attaaatgtt tctccagaag ttacttgctt tgcctccccg gtggacagat
     3061 caaatggtac atgccaatga tatttcattt tattatcgat tgccggctga taaccgcgac
     3121 ctttttccgt atggacgtga agtacaatcg gatgatcaat atcttttact tcttcaaata
     3181 aacggatcac cgtttccaga tcgtttccat tatccacgta acgataatca aatcccattg
     3241 ctttaaatag gttattgctg cttttaccgt tggtttgacg aagctcagct aagtttttat
     3301 ataaactgcc atgattctca tcaatagaca tctcattatc gtttactaat acgattagat
     3361 tggattgtaa aacaccacca ttgttcaatc cttcaaaagc taagccacca cttagggagc
     3421 cgtcaccgat aaatgctacc acatttcctg attctttctt cagatctctc gctttagcta
     3481 aacctgttgc taagctgata gatgtagatg tgtgccccac tgtaaaatag tcatgtttac
     3541 tttcatgttg gttggaatat ccgctcactt cacggtaatg tcctttttca aaaccatttt
     3601 ttcgaccagt taaaagctta tgaccataag actgatggga aacatcccaa acgattttat
     3661 cctttggtga atcaaataca taatgaaacg caatcgttaa ttctactaca cctagattcg
     3721 gaccaacgtg cccacctaca tcgctgtcct tttgcaagat tttttggcgc atctcttctg
     3781 ccaactgttt catttcctca acagataatt ttttgacatc tgcggggcta ttgattgcat
     3841 ccaatacatg taatactgta ctcataatac attctctcct ccgtactatc ttcttttaat
     3901 gaaaatagat ttgttacact ttatctattc tactacttag agttgactct aagtcaatag
     3961 cattttaaaa aaatttcaga gaaagcttgt
//
LOCUS       DAZKMZ010000143         3955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000143 DAZKMZ010000000
VERSION     DAZKMZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.148"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACG8M5_002671"
     CDS             complement(243..392)
                     /locus_tag="ACG8M5_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359861.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACG8M5_002672"
     CDS             854..1066
                     /locus_tag="ACG8M5_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359862.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ACG8M5_002673"
     CDS             complement(1344..3143)
                     /locus_tag="ACG8M5_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0359863.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ACG8M5_002674"
     CDS             complement(3267..3863)
                     /locus_tag="ACG8M5_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0359864.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1416 a          701 c          682 g         1156 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaaca
//
LOCUS       DAZKMZ010000144         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000144 DAZKMZ010000000
VERSION     DAZKMZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.149"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8M5_002675"
     CDS             complement(245..466)
                     /locus_tag="ACG8M5_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359865.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8M5_002676"
     CDS             complement(564..809)
                     /locus_tag="ACG8M5_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359866.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8M5_002677"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8M5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8M5_002678"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8M5_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0359867.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8M5_002679"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8M5_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0359868.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8M5_002680"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8M5_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0359869.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKMZ010000145         3733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000145 DAZKMZ010000000
VERSION     DAZKMZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.150"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..863
                     /locus_tag="ACG8M5_002681"
     CDS             111..863
                     /locus_tag="ACG8M5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0359870.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            860..1774
                     /gene="pfkB"
                     /locus_tag="ACG8M5_002682"
     CDS             860..1774
                     /gene="pfkB"
                     /locus_tag="ACG8M5_002682"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0359871.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1792..3723
                     /locus_tag="ACG8M5_002683"
     CDS             1792..3723
                     /locus_tag="ACG8M5_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0359872.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1198 a          663 c          846 g         1026 t
ORIGIN      
        1 gttttttatt tttttttgac tgatttataa tttttatgaa cgtttttgac tgatttatat
       61 tgctagtaga aaaaaaatat gctattattt gtattgaggt gatcgtgaaa atgcttacag
      121 aggaacgcag acaaaaaatc ctagacttgc ttgaacagca gaaaatcgtg aaatcacaag
      181 atcttgtaaa ccttttgaat gcttctgaat caacgataag acgtgattta caagagctgg
      241 agaatagtgg gtttctagaa agagttcatg gcggagctaa gaaagagcaa ttattgaatt
      301 ttgagcagaa tatgagtgaa aaatcactca aaaacgttca cgaaaaacag caaatcgcca
      361 aatcggcagc tgaaaccatt aatgacgaag atgtcattta tttagacgct ggctctacta
      421 cgttagaaat gattcctttt ctgcaaggaa aacagatcac agtagtgaca aattccgttc
      481 aacatgcggc aaatttagtt gatttggagc ttgctaccat tattttaggt ggaacgataa
      541 agctggcaac taatgctgtt ttaggatcta atgcgataga acaactaaaa aattttcatt
      601 ttaataaagc atttatggga atgaacggtg cccatcttga acaaggattc acgactccgg
      661 atccagaaga ggcagctgtt aaacgtttag ccatgaaaaa ttccgaagag acatttgtat
      721 tgattgatca taccaaattc aaccagttgt ccttcacctc tgttgcacct ttggaagcag
      781 ccacgatcat aaccgagcgt tgtccattag aattcgttaa tgaattccgt gaaaagacaa
      841 caattaagga ggcaatacaa tgatttacac agtaacactt aatccatcaa ttgattatat
      901 cgtacgcgta gatgggttaa aactaggcga tttgaaccga atgaaggaag acttcaagtt
      961 acctggagga aaagggatca atgtttcccg tattttgaaa cggatcgatt ctcaatctac
     1021 agcactaggg tttttaggag ggttcactgg cgaatttatt tcgaagtggc ttgaaaaaga
     1081 acaaatccag tctttgttca caaccgtaaa agaagataca agaatcaata tcaaactaaa
     1141 atcagacact gaaactgaga tcaatggctt aggcccatct atttcagatg aagaaatcga
     1201 tgagttgaag caagcattag gaacagtagg acaaggagat atcgtcgtat tgtcaggaag
     1261 cgcgccagct agcctgcgaa aaggatttta tcaagaactg attgatatca ttcgagaaaa
     1321 aggtgcagat ttcgtgattg atacaacagg tgatgaccta aaaaatgcac ttgttaagaa
     1381 accgttgttg atcaaaccaa acaatcacga attagctgag ctttacaaca caaccttcca
     1441 atctgtagaa gaaatcattc cgtatgggaa aaaattacta gctgaagggg cacagaatgt
     1501 gctgatctca atggctggag acggtgcttt gctcttcacg gaacaaggaa cttataaaac
     1561 gaatgtatta gttcgcccat tgaaaaattc agtaggtgca ggggattcaa tgattgctgg
     1621 ctttattggg gcttacagcc gtacgaaaga tccaatcgaa gcctttaaat ggggagttgc
     1681 ttgcggaagc gcaacagctt tttctgatga cttggcagct cgcgaattga ttgatgagtt
     1741 gctgccagaa gttgaaatca caaaaattga ttagtttcaa ggaggataat catgaatatc
     1801 aaggatttaa tggtcaaaga agcgatgatc atggatttgc aagcgactga taaaaaaggc
     1861 gcaatcgatg aaatggtcca aaaaatgttt gatgccggcc ggatttccga tatcgaaaca
     1921 tataaagaag gaattctagc ccgcgaagca caaacttcta caggtcttgg tgatggaatc
     1981 gccatgcctc acgcgaaaaa cagtgcagtt aaagaagcaa cagttttatt cgcaaaaagc
     2041 caaaaaggag tagattatga agccttagac ggacagccaa cttatctgtt tttcatgatc
     2101 gcagctccag aaggcgcaaa cgacacacat ttgcaagcat tagcagcact atctcgattg
     2161 ctgattgatg cagatttcgt aggaaaatta aaagaagcaa atacaccaga agaagtccaa
     2221 gacttgttcc aaacagctga agagcaaaaa gaagcagaag aaaaagcaga acaagaaaca
     2281 caagctgcag aagcttctgc tcatccagaa agaaaattcg ttgttgccgt tacagcttgt
     2341 ccaacaggga tcgctcacac ttacatggca gaagatgctt tgaagaaaaa agcgaaagaa
     2401 atgggtgtgg agatcaaagt cgaaacgaac ggttcagaag gaatcaaaaa ccgattgact
     2461 gctgaagaca tcgcccgtgc agaaggtgta atcgttgcag cagacaaaaa agtagagatg
     2521 aatcgctttg acggaaaaga actgatcaat cgtcctgtca gtgatggtat ccgtaaacca
     2581 gaagagttga tcaatctagc ccttagtggt actgcaccag tattccatgg tgatggaaaa
     2641 gaaagcagtt cagaagaagg aaatgcggat ggtactatcg gacaaagaat ttacaaagat
     2701 ttgatgaacg gcgtatcaca catgttacct ttcgttattg gtggaggaat cgctattgcg
     2761 ctttctttca tgatcgacca atttattggc gtgccacatg atcaattatc aaatctaggg
     2821 aattacaatc aagctgctag ctggttcaac caaatcggac aagctgcatt tggtttcatg
     2881 ctgccggtat tagcaggatt tattgcttca agtatcggtg accgccctgg tttgatcgtt
     2941 ggatttgctg ctggtgcatt agctaataca ggcggtgcag gattcttagg tgcactgatt
     3001 ggtggattct tagctggtta tgtcatcgta tttttacgta aattattcaa gaacttacca
     3061 aaatcattgg atggaattaa aacgatcctc ttttatccag tgtttggctt actgattact
     3121 ggtttcttga tgttgctggt caatgtccct atgaaagcaa tcaatgatgc attgaacggt
     3181 ttcttgacaa gtatgagtgg ttcgaatgct gctttacttg gtgcattatt agctggaatg
     3241 atggcggcag atttaggcgg tccaatcaac aaagccgctt atgtgtttgg tacagcaact
     3301 ttagctacga ctgttgcaac tggcggaagt gtcgtgatgg cttctgttat ggctggagga
     3361 atggtacctc cattggcaat tttcgttgct acacgtttgt ttaagaataa attcagcaaa
     3421 acagatcaag atgctggttt gacgaacatc gtgatgggac tatcgtttgt cacggaagga
     3481 tcgattccat ttgcagcagc tgacccgatt cgtgccattc caagttttat tattggttct
     3541 gctttaactg gcggattagt cggagctttc ggaatcaaat tactggctcc tcacggcggg
     3601 attttcgttg tattcttact gagtcacccg gtcatgtatt tggtgtttat tgctatcgga
     3661 gcagtcgtat ctggtgtgat ttatggttta ttgagaaaag ctcctgcagt agcagaagca
     3721 tagaaataaa aaa
//
LOCUS       DAZKMZ010000146         3710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000146 DAZKMZ010000000
VERSION     DAZKMZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.151"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8M5_002684"
     CDS             131..505
                     /locus_tag="ACG8M5_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0359873.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8M5_002685"
     CDS             complement(619..1248)
                     /locus_tag="ACG8M5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0359874.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8M5_002686"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8M5_002686"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8M5_002687"
     tRNA            1668..1741
                     /locus_tag="ACG8M5_002687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8M5_002688"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8M5_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359875.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8M5_002689"
     CDS             2338..3666
                     /locus_tag="ACG8M5_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0359876.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT         1266 a          577 c          693 g         1174 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct
//
LOCUS       DAZKMZ010000147         3697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000147 DAZKMZ010000000
VERSION     DAZKMZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.152"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..1111)
                     /locus_tag="ACG8M5_002690"
     CDS             complement(407..1111)
                     /locus_tag="ACG8M5_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359877.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1298..1543)
                     /locus_tag="ACG8M5_002691"
     CDS             complement(1298..1543)
                     /locus_tag="ACG8M5_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359878.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1560..2411)
                     /locus_tag="ACG8M5_002692"
     CDS             complement(1560..2411)
                     /locus_tag="ACG8M5_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0359879.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2577..3581
                     /locus_tag="ACG8M5_002693"
     CDS             2577..3581
                     /locus_tag="ACG8M5_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0359880.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1156 a          627 c          719 g         1195 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaaccg cttttttctc tatcagaaaa tatgtttatt attagaattt
      181 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
      241 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
      301 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
      361 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
      421 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
      481 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
      541 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
      601 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
      661 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
      721 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
      781 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
      841 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
      901 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
      961 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     1021 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     1081 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     1141 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     1201 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aaaaatggaa
     1261 cggattatgt atatatacat aatccgttcc attctttcta ttttttcggt atcaaggcaa
     1321 aaacaggtag ttcaggtgaa taaatgactt catgttcgtg ccagggtttg accattagcg
     1381 aatgatcgaa ccgttttttc agttcttcga tgatttgatc atgtgtccag tttcttgcag
     1441 gaaaatgttg gattttctct gcatcgatca aaaagacaaa atcggaatga aaagtctggg
     1501 taacttcttc cgagagagaa ttcaagcgtt cgattggatc cataaaatca cctttcgact
     1561 taaaattggg tagtatctgg gtcttttgct ttgccgacta caccatctaa ctgatcgatt
     1621 tgtttcattt cttcttctgt taattcaaaa tcaaataatt cagtattttc tttgattctg
     1681 cttggtgtca ctgacttagg aagaggaagg aattcgtgtt gcagcgacca acgcaaagca
     1741 acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt tcatttcagg cacatcgaag
     1801 attttaccag tgcctaatgg actatatgct tcgaggatca tatcatgttt ttttgcatac
     1861 tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa cttgattgac catcggcata
     1921 atttttgctg tttttgctag tgtatctaga tggtggggta agaaattgct cacaccgatt
     1981 gctttgattt tacctgcttc atacagttct tcaaatgctc gccatgtatc tgcatttgct
     2041 tgttcccaat tatcacgaaa agcaacaggg tttggccagt ggatcaaaaa cagatctaaa
     2101 taatctgttt ggagcttctt caatgattct tcaaaagaag acatcacaag ttcatagctg
     2161 tggtttgcat tccataattt tgttgtcaag aatatctctt ctctaggaat tccactttcc
     2221 ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt gggcagtatc gatatggcga
     2281 taacctgcag ccagcgcttc tttaacagag gaaacagcga catccccatc aggtgtttgc
     2341 catgtgccga atcctactac cgggatttcg taaccatttg atagacgata agtgcttgtt
     2401 aatgaattca tactttcagc tcctttttta ttcgttcaac tataagtgta gcgcattttt
     2461 cgaaaaaaag aatccatttt gtttctactc ttgaaatttt tgtttaaaag aaaatgattt
     2521 tgattctggt atttttgatt ttttttccgt aaactaagac agaagggaat gagaaaatgg
     2581 atatctattt aaaaaaagca attctgcata ttattgacag agaaactggt tctccagtct
     2641 tttcacaaaa agagctggat ctgacaaaag aatatattcg tgattttctt caaaagaaga
     2701 tccaaaaaat ctcttctgct caaacaaaaa ctggacaact agcagaagaa agcatgatcg
     2761 ttgaatcact taaaatcgct caagaaaatt ttgtggaagc aagtgaagca atcgttcagc
     2821 ggtggttcga tatctaccaa gaaagtgaag aagcacctag tgcagatgta tttgttgtct
     2881 tgtatgaatt agatacagtg atgtacttat ctttattgaa agtcaattat cgtgaggctt
     2941 acactcactt tgttgaagca gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag
     3001 caattctagg ggcgaaaaca caaaaagcag atgaagcatt cgctgtgaat ttgactgatt
     3061 ggacctacga attaattgaa aaaaaatatg agttttctgg caaaaaagag ctttattttt
     3121 ctggaagagt gatcgaaagt gttcctgcac cttctttaga agaaaatgta aaagtgatcc
     3181 aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga agaatttgat atcatggctg
     3241 atttgaaaga agctgtctat gatacgatag aagaaaaagg tcggttggat catgagatga
     3301 ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac
     3361 aagagcaagg atttgtccca gaagcacctc ctgtaaaaga agtacaagaa atttctgaga
     3421 aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat cgaactgatc gtaccagtag
     3481 atgtatatcg caacccagat ttgattgaat ttgtgaataa tccggatggt acgatatccg
     3541 tgatgatcaa aaatgtggat gaagtattga atcgtttata aaaatgacta gaatattagg
     3601 tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga
     3661 atataacaaa aaaagagcgt gagacaaaag taaagaa
//
LOCUS       DAZKMZ010000148         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000148 DAZKMZ010000000
VERSION     DAZKMZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.153"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8M5_002694"
     CDS             <1..277
                     /locus_tag="ACG8M5_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0359881.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG8M5_002695"
     CDS             420..641
                     /locus_tag="ACG8M5_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359882.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG8M5_002696"
     CDS             657..1499
                     /locus_tag="ACG8M5_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359883.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG8M5_002697"
     CDS             1562..3364
                     /locus_tag="ACG8M5_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0359884.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG8M5_002698"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG8M5_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0359885.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKMZ010000149         3660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000149 DAZKMZ010000000
VERSION     DAZKMZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.154"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..922)
                     /locus_tag="ACG8M5_002699"
     CDS             complement(<1..922)
                     /locus_tag="ACG8M5_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0359886.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKII"
     gene            complement(926..1336)
                     /locus_tag="ACG8M5_002700"
     CDS             complement(926..1336)
                     /locus_tag="ACG8M5_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0359887.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1354..1845)
                     /locus_tag="ACG8M5_002701"
     CDS             complement(1354..1845)
                     /locus_tag="ACG8M5_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0359888.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1855..2646)
                     /locus_tag="ACG8M5_002702"
     CDS             complement(1855..2646)
                     /locus_tag="ACG8M5_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0359889.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2639..3412)
                     /locus_tag="ACG8M5_002703"
     CDS             complement(2639..3412)
                     /locus_tag="ACG8M5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0359890.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1137 a          770 c          501 g         1252 t
ORIGIN      
        1 caattatttt ttcaggcagt ttaacaccaa gagttttgtc tgtcttttga ataatttgat
       61 gtggaacaag tgcaccgccc caatcccagt tattttgatc gttctcccct tcttttgttg
      121 ccacccagcc agttagaaaa cggccagttt cattagttcc gactgttctt gctgcataat
      181 aggcttttcc gtcgaaagct tcgtctactg gtgatttcca atctgaaaat aatcctgctc
      241 cgactcgata tttagtcttt ttatcttcac aatactctga aaaaagtaaa taccacgttc
      301 catcaatttc aaatatatca ggcatttcaa tcatgttaaa ttcattatta gcccagtagt
      361 ctcctttgaa attccaatct tttaaatctt cggattcaaa atagaccaat ctccccgtcg
      421 gctttttctt acttgctggt tttctagctc ctagtatcaa aacgtatttt ccatgatctt
      481 gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc atatccttct tgaggaacaa
      541 gttttgctgc ttcgccatcc tttttccaat gaattaaatc tttagaactt gcaatcatta
      601 acacttctga agtttttcca tccaacttag ctttgcgatt atgtccagta tacaaagcta
      661 aatactcatt gattccttgg acaatacttc cagcgtaaat gtattgatca atgtcatcgt
      721 tgccaccacg atgaattact tccccgtatt cttcaaattt cacaaaatca gtggttgtaa
      781 ctagtgacca cccaaaagga tcagttaaag gtttaggatt tctcgtatct cgttgatgaa
      841 atagataaaa agttccatct ttataaaaag gcatgcaatc cccaaaccaa aaatctttag
      901 gcttgtaaaa aattttttgc atttatcact catcctcttc gttagtatgt ctttgcatat
      961 ctgcaagaca cgcaattgta ttcatatttc ttagattttc gatgatatca tcgctttttt
     1021 ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc taaattcatg ccaccaagaa
     1081 gttgaaaccc attttgaaat acttcacttc ttttagcaaa ttcgttgaaa ggtgttccac
     1141 caatcaagtc agcaaatacc ataacttctt gattttttac ttgagaaact attttttcaa
     1201 tgttttcacg gaattcttca atgcctgcat ctccaagtgt taccacatga caatttaaat
     1261 tatcgccaaa aaacattttt aatgtatcta caaatccaat ggcaatattt ccatgagatg
     1321 taattataaa ctccatccac tcatcctctg ttcttaataa ataaaatcct ttaaaaattc
     1381 ttttgaatct ctaggagttt gtatcactgt cacttcaact ccatcactaa ttagttcttt
     1441 aaaagcctct ctctcttctt cagtaatatt tatcgtcgga cggatgcgtt ctgttccatt
     1501 ccttcttgat aaatttccta cgtttacact tgttattggt aaaccgttat caatcaaaaa
     1561 tttaacatcg attggagaaa ctgcaacaat aagaactttt tgtccttcat atttaccaga
     1621 aagaatattt ttaactgcta cttcccttgt aattattgaa gttctaacat taggtggtgc
     1681 agcgaggcga actacagctt tttttacgtc atcattggct acttcattat taataaccat
     1741 aattctgtta gcactcaatt gatttgtcca ttgtgttgct acttgtccat gaatcattcg
     1801 atcatcaata cgaacatgta taataccttt cattttttca tccatattca acacctactt
     1861 caaaatattt aacgctctta acgcaataga aactactaag attaatagaa ttacataaac
     1921 tggtttaacc tttcgtccta acaaataata cacaattgct actacacatg caggtaataa
     1981 accaggcata acttgatcaa agatttcatt tccggtcatt gttaagtcgc cttgtttaaa
     2041 ttcaaagcca acattcactt ttacaactgt ggcaatgagt gcaccgacca ccattaaccc
     2101 taaaattgat actgcatcag taatagactt taattttgtt tcaatttgag ttagtaggct
     2161 cgcacccgat tctttcccag caaggtataa aggataagca gtaatcctta ataaaattga
     2221 tgccagtatc cacaaaataa ttccaacaaa actaccttcc aatgccatat tagctgcaat
     2281 tgctccaaaa atagtccatg gaataactac aaatacacta tctccaattc ccgctaaagg
     2341 acccattaga cctgttttca gagcacttac tgcttccaaa gacttatttt tttcttcttt
     2401 ttcaattcca acattcatac caataatgta aggcgctgta tacggcgtag tgttaaaaaa
     2461 ttgtaggtga gctaaaacgg attgtttcat tccttcagga tcatctttat atgtgtcctt
     2521 gataaaagga agcatagaat acgcataacc caacccttcc attttttcat aatttaatga
     2581 agttgcacca ttgagaatcc accggaaaat tacttgtttg tcgatttttg tcagtttctt
     2641 attcatcttc gtcaaaacct cctgttgcag ctggtgctac ttgagtatta tttgatctat
     2701 ttttatatac gattagtgcg attgcagcgc caagtaacga aatccctaaa attggtattt
     2761 ggaaataaac tgccaaaacg aaaccaacta acaaataggc aaagtaatct tttgtaggta
     2821 aatatttaag tagcattcct atcccaacag caggtaatag tccccctgct acctttaaac
     2881 caccggtaac ccattcagga atataattga ttgcacttcc cactaacgtt gggcctaata
     2941 tcacagttaa caaaacagga attccagata caaagcttgt attaattgat gccaaatatt
     3001 gcatccactc taatttttga taatcttctt tttgtgcgaa tttttctgcc ttttgttgaa
     3061 aaaaaatgtt tgttgaccac tttagaacat ctagttgtac aattaacatt gctaccggta
     3121 ttgctaaagt tattcctata gaagcatcct ggttagttga gattgctaaa taagtaccga
     3181 tcaatgctgc tgtttgataa tccggtactg atgcaccacc aaaactagta atacctagtg
     3241 tcattaattg caaagtccca ccgatataaa gtccagtttg aatatctccc ataataagtc
     3301 cggtcaagaa acctgcaacg acaggttggg aggttccgat tttgggccct tccttatccc
     3361 agttaatgaa aaatccatac aacacaacta aaaatgtttg ccaaaacgcc attttgttct
     3421 ctcctttctt cttatacttt tatataagca attactgtgc caactaacat tatccaataa
     3481 agcgggaaaa tacagtaaat attaattaaa tgagcgtcta aatatacttt ggacaccctt
     3541 tcattttttt agacgctagt atgctattta gaaaatagtc tgctcaaaaa ttgtatagat
     3601 taactcatac tctcctttgg gtatttcaat acagtaaata tctttgattt ctgaaaaaaa
//
LOCUS       DAZKMZ010000150         3643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000150 DAZKMZ010000000
VERSION     DAZKMZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.155"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8M5_002704"
     CDS             <1..342
                     /locus_tag="ACG8M5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0359891.1"
                     /translation="FSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWRLAQ
                     KTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKYDLE
                     TGGNDNEYFND"
     gene            323..1039
                     /locus_tag="ACG8M5_002705"
     CDS             323..1039
                     /locus_tag="ACG8M5_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0359892.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            1039..2487
                     /locus_tag="ACG8M5_002706"
     CDS             1039..2487
                     /locus_tag="ACG8M5_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0359893.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2557..3066)
                     /locus_tag="ACG8M5_002707"
     CDS             complement(2557..3066)
                     /locus_tag="ACG8M5_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359894.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(3056..>3643)
                     /locus_tag="ACG8M5_002708"
     CDS             complement(3056..>3643)
                     /locus_tag="ACG8M5_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359895.1"
                     /translation="NQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLF
                     IFFFYYSVRQNKQELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVY
                     EALLQRINFLVLKTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFA
                     QTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1265 a          625 c          750 g         1003 t
ORIGIN      
        1 ttcagtattg gtatcttatt cttaataatt ggcagtcttt ttcggattct gccctcaaaa
       61 ggaaatctgc ctttttatgg gtatcattcg cctcttgccg caaaaacaga cgctcattgg
      121 cggctggctc aaaagacaag cggaaactgg ttttttctta tgggcttact gatggcattg
      181 atcggctatt atttaaagac atctgggcat acgaattatt ttctgattga aatgcttctg
      241 ctagtatttc caattatgcc tatttttatt atgacagaaa agaaattaca gaaatatgac
      301 ctggaaactg gagggaacga taatgaatat tttaatgatt gaagataatc aatctgtttc
      361 tgaaatgatg cagatgtttt tcttaaatga aggttgggaa gcaactttca agtatgatgg
      421 aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat tgggatatga ttacactaga
      481 cctgaatttg cccacactgg acggtatgct agtaggaaga gagatccgca aagtttcaag
      541 tactgtgccg attatcatgc tgacagctag agattctgaa agcgatcagg tgatcggctt
      601 agagatgggg gcagatgatt atgtcacaaa acctttcagt cctctgacgc ttattgctcg
      661 aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca agagaagaag cagtagtttc
      721 agatgatcat tttgatatcg agactagtca tttcaagatg aatacgaaaa cacgagaagc
      781 gtatctggac aaccggccaa ttgaaggact gacgcctaaa gagttcgatc tattgtttac
      841 tttagcaaaa aaaccacgtc aagtattctc tcgggagcag ttgcttgaat tagtctggga
      901 ttatcagtac tttggtgatg aacggacagt ggacgcccat atcaagaaat tacgacaaaa
      961 aatcgaaaag gtcggaccac aagtcatcca aactgtttgg ggagtcggct acaaattcga
     1021 tgattctggt gttgcctaat gcgttatttg tatcagcaac tcttagcttt ctgcggaatg
     1081 gtcgcgttga tcttattgat cgttggagtt tcttttacac aactgacaaa acaaacgatt
     1141 gaagaaaaca attaccagca actgtttgga tatgctgaat cagttgaaaa gacaacgcaa
     1201 acctatgccg atacgctccc gcagtttagt caagatcagg cgtttcaaaa tgcattgctg
     1261 ctgacagagc aagtactgac agaacaaaat gtgaacttta tatttatcga taaaaacgaa
     1321 cgtgtcattt atccgactaa tacagcttta ctgaatttta ccttaacagc tgatcaatgg
     1381 aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga ataaaaatat tttaggacaa
     1441 gcacaggcga cctcttacgc acttgtgcca tttaatttga atcatgaatt ctatggagca
     1501 cttgtagtta gtcaacctgc gcgaaatatc gataacagcg ttcgtccaat tacactgaac
     1561 ctgttcaaag gatttatttt ttccagtatc gttgcagtta ttgccagcta tgcttttgca
     1621 gcattccaag ttaagcgtat caatcggtta aggaatgcga cgaaagaagt gacgaatggt
     1681 aattttgatg tacagctccc cgtacatgac aaagatgaat ttgatgagtt agcagatgat
     1741 ttcaataaaa tgacgaattc attgaaagaa tctcaagcag aaattgaaga acaagaaaat
     1801 cgtcgaagac aatttatggc agatgcttct cacgagatga gaacacctct tacgacaatc
     1861 aacggacttc tggaagggtt agaatacaat gcaatccctg aaaatcagcg agaaaacgcg
     1921 attaaattga tgaaaaatga aacagagcgt ctgatccgtt tggtgaatga aaacttggat
     1981 tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta aaaaattcaa tggaacagaa
     2041 acattgaaaa atattgttgc acagttagaa gctaaagcag aagcagcagg agatacattg
     2101 acattgaaag ccgttgatga tattgatgtt tacgctgatt atgaccgatt tgtccaaatc
     2161 atggtcaata ttattcaaaa cgcgatacag tttactgaaa acggccaaat cacgattact
     2221 ttagaaaaag gctatcttga aacgatcatc acgattgaag atacgggaat tggaatgtct
     2281 gaacaacaaa tgaaaagtat ctgggatcgt tattataaag tagatccttc aagaaaaaat
     2341 acaaaatatg gtgaatcagg attaggttta ccaatcgtgc agcaacttgt acgcttgcat
     2401 aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa caaaattcgc tgttcgtttg
     2461 cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta taaaagatca tgaatcgatc
     2521 ttctataccg cttgttttta ctataaaatg attgatttag cccgcgggct ctaaacgatc
     2581 aatataagga ctttgagcaa tagtagcatc atgagtaaag agtaagatac cttgattttc
     2641 ttcatgagct aaataagaaa gcaagtggag tagctcttgg atctccaata cactaagttc
     2701 atcaaatgta tcttcaataa caatattgtt tcgcttggaa agcaaggcat gaagtaattg
     2761 cagctttact ttttcaaaag acgtcaactc agctgccgca caattaagca aggaaggaga
     2821 gatatcaagt ttttcttgat aggccaaacg atcttgtttg atttcttttt ccggtacatc
     2881 caacaggaca tggtctttaa gattcagata aggaaacatc ggccaatttt tttggatgat
     2941 cgcaaaatta tccagttttg caacaagaac ctcttttttc aagtgctcca atattttatg
     3001 ctgtttttcg agaggacaca aaataacggt caagtgttca gcatgaatca aatattcagg
     3061 tttcattacg tccaggcctc ctactttgca aatgatggat aaaaaaagtg aaagggaata
     3121 gactaagaat gagacagcat ccactgagcc caataagagt ttgggcaaat cccttaagta
     3181 tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa aaaagtctgt tgatccatcg
     3241 gaattgtaag tccgcttacc gaagtcaaga tatcacggat atctggcagt tcagtagtct
     3301 ttaagacaag aaaattgatc ctttgaagaa gcgcttcata aactgactgg aagcttacga
     3361 tccacaataa aaccaagata caacaaagaa agagaggaaa gaacagctgc cagaaaataa
     3421 aaaggaataa tttggtttta gaaagaccag tcaggtgcca attgatcaat tcctgcttgt
     3481 tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa ggcaaataga aaaaataaac
     3541 tgaatatgcc ttttatataa aatcggctag ctaattctaa agccggaaga agctctgtag
     3601 atgcagaagc gccaataaat gaaagccgat cttggacttg att
//
LOCUS       DAZKMZ010000151         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000151 DAZKMZ010000000
VERSION     DAZKMZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.156"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8M5_002709"
     CDS             <1..186
                     /locus_tag="ACG8M5_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359896.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8M5_002710"
     CDS             329..553
                     /locus_tag="ACG8M5_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359897.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8M5_002711"
     CDS             568..1437
                     /locus_tag="ACG8M5_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0359898.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8M5_002712"
     CDS             1418..2152
                     /locus_tag="ACG8M5_002712"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0359899.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8M5_002713"
     CDS             2185..3093
                     /locus_tag="ACG8M5_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0359900.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8M5_002714"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8M5_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKMZ010000152         3593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000152 DAZKMZ010000000
VERSION     DAZKMZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.157"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACG8M5_002715"
     CDS             complement(<1..1838)
                     /locus_tag="ACG8M5_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0359901.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACG8M5_002716"
     CDS             complement(1835..2575)
                     /locus_tag="ACG8M5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0359902.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3593)
                     /locus_tag="ACG8M5_002717"
     CDS             complement(2565..>3593)
                     /locus_tag="ACG8M5_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0359903.1"
                     /translation="MIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNIWTGFKLT
                     LINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWIDLKYNAIT
                     TWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWT
                     NLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNI
                     KNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVN
                     GISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGA
                     VGGTLYAT"
BASE COUNT         1137 a          683 c          426 g         1347 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atattccaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtggtaatct tccgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattga gtgcagcaaa ataaatattt gtaaaataac ttacaaatat attctttata
     3541 gattcccaaa tatttatggc actttcttta atattattcc aaacagcaat cat
//
LOCUS       DAZKMZ010000153         3520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000153 DAZKMZ010000000
VERSION     DAZKMZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.158"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..455
                     /locus_tag="ACG8M5_002718"
     CDS             138..455
                     /locus_tag="ACG8M5_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0359904.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            452..613
                     /locus_tag="ACG8M5_002719"
     CDS             452..613
                     /locus_tag="ACG8M5_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0359905.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            616..915
                     /locus_tag="ACG8M5_002720"
     CDS             616..915
                     /locus_tag="ACG8M5_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359906.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            915..1229
                     /locus_tag="ACG8M5_002721"
     CDS             915..1229
                     /locus_tag="ACG8M5_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0359907.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1222..1752
                     /locus_tag="ACG8M5_002722"
     CDS             1222..1752
                     /locus_tag="ACG8M5_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0359908.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1749..2018
                     /locus_tag="ACG8M5_002723"
     CDS             1749..2018
                     /locus_tag="ACG8M5_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359909.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1948..2151
                     /locus_tag="ACG8M5_002724"
     CDS             1948..2151
                     /locus_tag="ACG8M5_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0359910.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2148..2435
                     /locus_tag="ACG8M5_002725"
     CDS             2148..2435
                     /locus_tag="ACG8M5_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359911.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(2468..2719)
                     /locus_tag="ACG8M5_002726"
     CDS             complement(2468..2719)
                     /locus_tag="ACG8M5_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359912.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            2922..3335
                     /locus_tag="ACG8M5_002727"
     CDS             2922..3335
                     /locus_tag="ACG8M5_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0359913.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1314 a          517 c          786 g          903 t
ORIGIN      
        1 taataaagta ttaaagtaaa aaataaaaag taaattatat aaaaaagaat aggtttttgg
       61 gtctgaccct ccaaactctc caccaaaatc ttcctaaatg ctttgatacc aacgattaaa
      121 cagtctggag ggttacattg aaatcagaac atcaaataca agacgagatc agaattgcct
      181 tatcaaaaca tggttgcacg atttttcgat ccaatgcggg acaagtgaca acgatcgaag
      241 ggcggcaatt ttacgggatg ccaaaagggt tcccagatct atgtggacat cgtaattctg
      301 atgggaaagc aatttatata gaagtaaaaa atgaacgcgg taagttgcgt gaagatcaaa
      361 aaaggtttgc tgaattttta aacggtcaac ctgtactata cggtgttgca cgatcagtgg
      421 aagaagcatt gaaaattgtg gaggaatcat catgacaaca gaagaagtga ttcaaatgcg
      481 tattcgaagc cttcagcgtg agattgacga tctggaacgg acaaaggcag tgatggtcaa
      541 tgaaacggcg aggaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccatcg
      601 attggaggaa tgagcgtgga taagaaagca gcaatgaaac gaatcatcga actgacacat
      661 tctgagaatt ggcaagaaga caaagaaata gttgcagaag tccaaaagct cggtaaatca
      721 atgtggagtg aaaaaaccaa acggagaaca ccgagaaaaa ttgcaatctg gcatggtgac
      781 cgaattctag taacaggtac tgctgaacag ttatctgaaa ttactggatt aagcaaaaac
      841 attatctggg atagagctaa acgcgagaac gttgattcga aaggtcgtca atttaaacat
      901 tgggagaaga aataatggag gaactaatca caaaagtaga gcagtgggca aaagataagg
      961 gattggatca agctgattcc agcaagcaaa tgttaaaaac gatcgaagag attggggaag
     1021 ttgccgcttc tctagctaga aaagatgaat atggtttaag agacggaatt ggagacgtag
     1081 tagtaacctt gattatttta gctatgcaaa atgatatgga tttgtacgag tgtctgaacc
     1141 aagcatacaa cgaaatcaaa ggacgcacag gaaaaatggt agatggtgta ttcgtgaagt
     1201 cgagtgattt ggaggacagc gatgaataaa aaagtattaa ttgataaaca agcattgatt
     1261 gatgaattaa tgaaaatacc tggtgtggga tctaatagtg acgctttaga aacgattaaa
     1321 cgtttcccat cgtatgaacc gcagaagcca gttgtgccta agtttgtggc ggaactactc
     1381 gactattatc gtcagtcaac agatgttgat ttattagctt tattgataac tttccatgat
     1441 tggtattatc gcaaaactaa agacggtgag catgaagaag caattgattg gctagttgat
     1501 catcctgaaa tttatatgcg tgcatggctg tttggctacg agctcgagaa agagccgttg
     1561 tattgcgtaa taatcaatgg tagatatctt gtcaaagtat tcagcaatac aaacgttgtc
     1621 atattagttc cagcagatga attttcacag tacgttactc aaaaatttca attaactgaa
     1681 aaacaaatca aagcaattga tgaaagatac tggccgtttg ctgtgccagt ggatgaggtg
     1741 gtagaaggat gagcgaccca tacattacta aattagaaaa gttgtccgaa gattttgaga
     1801 agaataaaaa taaactaact aaagataaac ttatagaact ggttaggttg gttatcagag
     1861 gagctattac aagagaaaag gcgcagcaaa acatagaaat ttttgctcgt agtgtccgag
     1921 atggattaga gaagaaagtt aaacaaaatg aacaacagac accgcagaat aacaaaacta
     1981 agaaaacggg aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca
     2041 gaagaaacat ataaagtggc aagtcagtgt gttgctgatg cgagcggcgc tattcgtaag
     2101 tttggaattt cgatattaag tgatgatcgt aaatgggagg aaaaagaatg aaactaaaag
     2161 acggatttta ctccagcagt catggtatcg gcggtttaat gctagatatg ccgacaaaga
     2221 accctaaaac acgtgagaaa ccaaaattca aagtcggtga catggttcgc tgtgaagcag
     2281 aaggattcat ctatccattt tgtggatatg tagaacatct ctataatcac tcagcaatca
     2341 ttcgtattga aaacacgatg gaatgtgaca agtggttagc gaaaagcaaa gagaatttag
     2401 cagtagcgag attggtggat atggaggtta tataaacagc gaaaaaatgt acttctaaga
     2461 gaaaaattta ttttttataa aaataggaaa gtatcattat gaaaaagacc acagagataa
     2521 ataaacctac cacataaaaa atgaatattt cttgcgattc aatataggta tatttaacaa
     2581 tttgtacaat aaaaaatact ataggtaaaa aagataacat gaggagctta tttgttctag
     2641 agtgtttgac aaagtaacaa aatgcgacga taccaataca aacgggaatc caaaataaca
     2701 gcatgctcca catagtcact caaccatctc ctatacgctt tattatcaat agataatatc
     2761 atttttcgta ataaagataa aaaaataagt aaggcttctc attaaaatat aaaaaagaca
     2821 gccgaccact gactgcctat ataagagtat tgaaataaaa agctgctgat ataataaatt
     2881 ccacaagttt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     2941 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     3001 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     3061 aggcgccgaa gcacggcaat aaggcagaag acgcaatcat tcagatgatg gatatagaag
     3121 cggagagaga cgcgattcta gcagccttga tggctcttag tctgattagt cgtcagatac
     3181 tctactatag cttttgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg
     3241 gttattcaga aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag
     3301 catataaaca cggaagaata attgcttata aataatttgg cggctttttg gcggaatgat
     3361 ggcggttttt agctatttac cagtgatatt atggtagtgt cgaaagatta gtgataggtc
     3421 taagacaaaa taataataaa aggaacatcg ttttattatt gtttcacaat taagcttcga
     3481 tagacagcaa cggaaatatt aagaataagg atgtgaattt
//
LOCUS       DAZKMZ010000154         3463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000154 DAZKMZ010000000
VERSION     DAZKMZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.159"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..912
                     /gene="cdaA"
                     /locus_tag="ACG8M5_002728"
     CDS             25..912
                     /gene="cdaA"
                     /locus_tag="ACG8M5_002728"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0359914.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            909..2078
                     /locus_tag="ACG8M5_002729"
     CDS             909..2078
                     /locus_tag="ACG8M5_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0359915.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2102..3457
                     /gene="glmM"
                     /locus_tag="ACG8M5_002730"
     CDS             2102..3457
                     /gene="glmM"
                     /locus_tag="ACG8M5_002730"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0359916.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         1106 a          639 c          830 g          888 t
ORIGIN      
        1 ctcaaaagag aagagaggct ttttatgtca tttcaaatcg gggaactttt taatttggac
       61 tattggcagc aaatgttttc cggtgacttg ttctctctga attttttggt gaacatactt
      121 gatattctgg ttgtctggta tcttgtatac aaactgatcc aactgctgcg aggcacaaaa
      181 gcgattcagc ttctgaaagg tgtagctatt tttattgtta tccgaatcgt agcggaaatc
      241 attggtctgc acaccttgtc ttggctgatg aaccaagtca ttacgtacgg ggtgatagct
      301 gctatcgtga tttttcagcc ggaaatccgg agaggactgg aacatcttgg acgcagttcg
      361 cttttcaaac aatcaaaaag cgaacagcag gaagatgaga agatgatctt gtcttttgac
      421 aaagccatcc aatatatgtc caagcgaaaa atcggtgcgt tgatcactat cgaacgtcat
      481 actggattag atgagtatat tgagacaggg attgctttag atgcagatat cactggggag
      541 ctgctgatca atatctttat tcctaataca ccgctgcatg acggtgcagt tattgtaaaa
      601 gaaggaaaga tcgcagtagc aagcgcctat cttcctctat cagaaagtat gttgatccct
      661 aaagaattcg ggactcgaca ccgggcagct gtcggtataa gcgaagtcag tgacgcgatt
      721 accatcgttg tctctgaaga aaccggggac gtcagcatca cgttagataa tgaactgatg
      781 gctggcttat cccagcaaga atatttggca atcttgcgta gagaactaat tgctgaagag
      841 aaaacaaaaa acaagaaaaa cgtgattcaa tctttcctag acggtatgac taagggaggg
      901 gatcgcaaat gatctcaaaa gagaaacgga caagcattct ttacagtttt attgcattgc
      961 tgttcagtat tgcgctgtac ttcaatgcaa atggccagtc cgttccaaat acgctttctg
     1021 gaaatgaagc gtataatcaa acagttacgg gggtgccgat caagatttct tatgattcaa
     1081 agcgctacta tatccatgga tatgaaaatg cagtgactgt caaactttcc agtgcaaacc
     1141 gagttcagtt gaatgcagag gcaaacgaag atacacggat gttccgcgtc acagcagata
     1201 tcactaagct gggcgaagga acacatgaag ttccgctaaa aattcaaaat ttgagcagtg
     1261 ctgtcacagc aaagatcgaa ccatcgacta ttacagtaac ggttgagaaa aaggtaataa
     1321 aagatttcga tgtggaaacg ttgattcctt caacgttcac accatctgga tatgaacttg
     1381 atgatacttc cgttagtcct aaaaaagtct ctatcactac aggtgacaaa acattggcag
     1441 aaatcaaacg agtagtagct gatgtggacg cttcgatggt cacagatgat gggattaata
     1501 gtgaggtccc aatacaggcg ctgaatgcag caggggaagt attaagtatc gtctctgatc
     1561 cagtgcaagt aacagtgaga gctgattcga tcaagccaag caagtcggta cgactttatg
     1621 ggatacagca aggaacacca gctgccgggg tagaaagcta tgatttcagt ttttcaaaac
     1681 tggaagcaga agtaagcggc tctagcgatt tgcttgcatc gatcggtgac agtattgctg
     1741 taccgatcaa tgtctcaggt atcaatcatc gaacaactcg taaaattgaa ataccagtag
     1801 aagaggggtt aagtatcaag cccgaatcag ttagtgtgga gattacaccc gtgttgcaaa
     1861 cgtcaagcag tagctctgct tcgacgagca gtcggacaca agaaaataca agtacgagta
     1921 gaagtatttc atctgctgat tcaggaacgg aaccatcgac aacaaccatt tcttccagtt
     1981 cggaatctgg gacaagcagc tcgaccacaa gtacagaaga aacatcagaa acaagcgaat
     2041 caacacaaat gagcagaaca aattcctcag aaaattaagt taaaggagta attttacaac
     2101 tatgggtaaa tattttggaa cagacggcgt acgcggtgaa gccaacaagg aacttacgcc
     2161 agaacttgca tttaagctgg gacggtgcgg aggttatgtc ctaagccagc atgaagaaga
     2221 aggaaaacgc ccacgcgtgt tagtagggcg ggatacaaga atctcaggtc aaatgttaga
     2281 agaagcgctg atttcaggac ttttatcggt gggtatcgaa gtgttccagc taggtgtgat
     2341 ctctacacca ggtgttgctt atctgacacg tttgcaaaaa gcaagcgcag gggtcatgat
     2401 ttctgcttct cataatccag cacaagataa tggaatcaaa tttttcggtg cagatggttt
     2461 taaactagta gatgaccaag agctagaaat tgaagcatta ttagatgctg aagtggatga
     2521 attgcctcgt ccatcagctg aaggattggg aactgtagaa gaatttccag aaggcttatt
     2581 gaaatattct caattccttg tacaaacgat taacggtgac ctttccggtt tgaccatttg
     2641 tgtcgatgca gcaaacggag caacttcaac aagtgttaat cgtttgtttg ccgatcttga
     2701 aacagatttt tatacaatgg gcacaaatcc agacgggtta aatatcaatg acggcgtagg
     2761 ctctacccat ccagaacgtt tagctgagat ggtcgttgaa aaaggagcag atgctggact
     2821 agctttcgat ggcgatggcg atcgtattat tgcggtggat gaacttggaa acattgtcga
     2881 cggtgataaa atcatgttta tctgtgctaa atacttggca gaaaaaaatc gattgaaaaa
     2941 agatacgatc gttacgactg tcatgagtaa cttaggattc cacaaagcgg tggaaggaat
     3001 cggtttgaaa gatgtcgtta cacaagttgg tgatcgttac gttgtagaag aaatgcgcaa
     3061 aaacgattat aactttggcg gcgagcagtc tgggcacatg attttcttag attttaacac
     3121 aactggagat gggatgcttt caggaattca gttactgaat atcatgaaac agacaggtaa
     3181 gaaattatca gagcttgcag cagaggtaac aatctatccg caaaaattag tgaatatccg
     3241 cgtaagtgat aaaaacggtg cgatggatgt tccggcaatc aaaaaagtga ttgaagaggc
     3301 agaagctgaa atgggatcag aaggaagaat cctagttcgt ccttccggta cagaaccatt
     3361 acttcgagtg atggcagaag cgcctactga tgagaaagtt aattactatg tcgataaaat
     3421 cgcggcagta gtcaaagaag aaatcggact ggattaagag ata
//
LOCUS       DAZKMZ010000155         3449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000155 DAZKMZ010000000
VERSION     DAZKMZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.160"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8M5_002731"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8M5_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0359917.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8M5_002732"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8M5_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          979 a          761 c          539 g         1170 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatcca
//
LOCUS       DAZKMZ010000156         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000156 DAZKMZ010000000
VERSION     DAZKMZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.161"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8M5_002733"
     CDS             301..585
                     /locus_tag="ACG8M5_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359918.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8M5_002734"
     CDS             588..1004
                     /locus_tag="ACG8M5_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359919.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8M5_002735"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8M5_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0359920.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8M5_002736"
     CDS             2522..2608
                     /locus_tag="ACG8M5_002736"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0359921.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG8M5_002737"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG8M5_002737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359922.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKMZ010000157         3198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000157 DAZKMZ010000000
VERSION     DAZKMZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.162"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8M5_002738"
     CDS             complement(<1..339)
                     /locus_tag="ACG8M5_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359923.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="ACG8M5_002739"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="ACG8M5_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0359924.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1119..1373
                     /locus_tag="ACG8M5_002740"
     CDS             1119..1373
                     /locus_tag="ACG8M5_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0359925.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1374..1691
                     /locus_tag="ACG8M5_002741"
     CDS             1374..1691
                     /locus_tag="ACG8M5_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0359926.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            2250..3185
                     /locus_tag="ACG8M5_002742"
     CDS             2250..3185
                     /locus_tag="ACG8M5_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359927.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT         1100 a          469 c          585 g         1044 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaagctt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat gggacaaaat aaaaaggcaa tacgccttag ttgtcggtct
      841 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
      901 ggcgacttct gcctaattct gttaaatgga tgttaacgga agtgtatggt gagtagttca
      961 ctcgtatttt gatatcgttt gtatatgttt tcagcgttct aagtggctta ttttgtccaa
     1021 taataatatg gagtggaatc ccttaagaaa caccgttggc aattgttgtt ataacatgtt
     1081 ataatttatt catcaaaaac taaaaggagt gtgataacat gggaaaagaa ctaaaggtta
     1141 gaaagatcgg caatagcgtg ggagtgatcc ttccaagctc tcttggatta aaaagcggag
     1201 acattatcca agccaaacaa gaaggcaact tattcatatt agatactact caaatagcaa
     1261 aagaacacga tcgcaaattg attgaagaaa gtttccaaga ctttgagaaa ggtttaacag
     1321 tttcagaaat tgaaatggta aaagctttcg gaaaatacgg ctggagtgaa tagatgaaag
     1381 agtacacgct agtttattct aaaagttttc agaccagcct tgaaacgata atagaagaat
     1441 gggaaaatga gcttgctctt tctccagcca gcataaaaag atttgtaggt gcgatccaga
     1501 aggctttaga actaaccaaa aattttccaa ctatgcatga ggaagtttcc gaaatctatg
     1561 gaatggatat cccaacatat cggatcgtga tcggcaaaag ctatgcctta ttttatcgga
     1621 tcgaccatga aaaaaaagag atacttgtag gacgtatttt ccaatctaaa caaatgaaac
     1681 taggctttta acaataatta ggaaaaagtc gctcatcctc tacaggtggg tgattaatgc
     1741 cgctcgatat ggttgttaac cgtcggttac tcgaagattg atatataaga cttagtacct
     1801 ttttattttg ctccatgtta tggccgaagg tctaaagata caatcattag tctagagaaa
     1861 aattaagaac cgtaggaact acggggatcg cttggtgaat aagagacact gctattagtg
     1921 aaaaaataag atatcaagta cgctctattc ccaaaaattt cgtgacttta ctcatgagag
     1981 gttcaagtag tatattttta atcaaaattg acgtaacttt taataaatac tacctaaata
     2041 aggtagtatt tatttttgtg ttttaaaaga ggaagtccaa tatatctatg ggaaacttct
     2101 taaatattat gttggtacat gaaaatatac atccctgcta gactatttac aacattttac
     2161 tttgtacgta ttcttgtttt aaaaggaatg ggataaaata taggtacaat aactatttga
     2221 tgctaaatat aattaatgga ggagagatag tgaacatact taatgaaaaa agatatgtat
     2281 ggtttgctgg taaaaaatat attactactt tcaaaagatt atccgagtta atgcagcaga
     2341 ctgttaaaag tgctatgcct gaaggataca agtttaattt tgttcaatct tcagtatcta
     2401 ctgaaagtat ctatttttca attctttctg agagtaggct ttttcatttt agaataagtt
     2461 ctcatgtaaa gaaaactgat gttgatatat atacgttccg tacaaataat tataaaaatt
     2521 taaatgattt gagtagaagt atcagtgaat atctcaaggg gaatagctat ctcaagattc
     2581 gattttcaca tatattaatt ttactaccat atttgaatga gaatttcacc ctaattcaag
     2641 tagataagtc tttcattgcc tcaggtaatc aaggaaatat gaatattaca agtaacaaaa
     2701 cgtcacgtgt gctattggcg ttgatgggtt acggaattct ttatagagat gaccaaaatg
     2761 gccagattct aataaatcgg gctgggcgtt cattattaat gaaatataga aaatttatac
     2821 cggaagattt tcaaaactta cagttagaaa acatgcgttt agaagattta tttagattag
     2881 cagaagatga taagtataga tttgaagttt tgaaaattca aaagaatgaa ctattgaata
     2941 agttatatcc tgagaatgcg ataaaagcac attttggtga tgctaatcat atgtatagtt
     3001 ggttcattcc taaaagattt agagggaaaa tcaatgctgg agatcaagtg aaagtactca
     3061 atcgaaatag tacagatgtc gtaatgataa cagatatcta tgtagaagat gaagatataa
     3121 tcaaatatat gaagcctgtg ataaaacttg ttaataaaaa taaaggaacc cgtggtgcta
     3181 gttaattttt tcctagag
//
LOCUS       DAZKMZ010000158         3182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000158 DAZKMZ010000000
VERSION     DAZKMZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.163"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(335..1309)
                     /gene="sgrA"
                     /locus_tag="ACG8M5_002743"
     CDS             complement(335..1309)
                     /gene="sgrA"
                     /locus_tag="ACG8M5_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0359928.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1505..2344)
                     /locus_tag="ACG8M5_002744"
     CDS             complement(1505..2344)
                     /locus_tag="ACG8M5_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0359929.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2529..>3182
                     /locus_tag="ACG8M5_002745"
     CDS             2529..>3182
                     /locus_tag="ACG8M5_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0359930.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIAFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     "
BASE COUNT         1036 a          670 c          471 g         1005 t
ORIGIN      
        1 cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct ttttatctat
       61 tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt
      121 tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt
      181 gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag
      241 ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg
      301 ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac
      361 gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt
      421 tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt
      481 tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt
      541 tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat
      601 tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca
      661 tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc
      721 ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc
      781 tactttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt
      841 cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca
      901 aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac
      961 tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg
     1021 tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat
     1081 cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga
     1141 ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa
     1201 atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa
     1261 ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc
     1321 tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac
     1381 gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt
     1441 tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca
     1501 tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta
     1561 acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa
     1621 agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt
     1681 cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat
     1741 gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac
     1801 ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa
     1861 gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat
     1921 gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa
     1981 aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca
     2041 aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta
     2101 aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga
     2161 ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa
     2221 ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa
     2281 ttcctaaaac aaaaccaact aagactatta aaacagatcc aatcaaccga ataatcctga
     2341 tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa
     2401 aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac
     2461 agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata
     2521 aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg
     2581 caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg
     2641 gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac
     2701 tattaaaaaa aatgattgcc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt
     2761 caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg
     2821 aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc
     2881 tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat ctagacactg
     2941 cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg actaaagaag
     3001 aagcagataa agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag cttgttcaag
     3061 cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca
     3121 atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt
     3181 ct
//
LOCUS       DAZKMZ010000159         3148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000159 DAZKMZ010000000
VERSION     DAZKMZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.164"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..966)
                     /locus_tag="ACG8M5_002746"
     CDS             complement(28..966)
                     /locus_tag="ACG8M5_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0359931.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1457..2674
                     /locus_tag="ACG8M5_002747"
                     /pseudo
     CDS             1457..2674
                     /locus_tag="ACG8M5_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2808..3047
                     /locus_tag="ACG8M5_002748"
     CDS             2808..3047
                     /locus_tag="ACG8M5_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0359932.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT         1143 a          506 c          529 g          970 t
ORIGIN      
        1 agcaattttt atcaaataaa acttatttta cttccaaaac ctaaattcac gttgccaaaa
       61 atcaatctgc ttttgcaatt gtttttcgtt cgcttttaaa gtcgatttca ttaattccgt
      121 taaatcaatt ggagatattt ctctaatcaa ttttttaaat ttagtcttag tattcttact
      181 tagctttccc cacatacttt cttcatgcaa caaagtataa accatagctt gctcattaat
      241 tttttctaaa gtagcccacg caggtttcaa gatgtgtaaa tcattaaaac aatcattcca
      301 gtaatcaacc atatctcttt ttaattcaac ttctacacgc cataaatgtt cagacacaac
      361 ttcaacatct gcgttatctt tacgttcttg ttttttatta taaattctaa taaatctatc
      421 actatcacgg acaccaaaat attttgtttc tggcttgcca ttacgaccat aaaaaacagt
      481 tttcttaact gctttatcag tcattgcata gtaatcgctc aaatcatctt caaaatcaaa
      541 agctaagtct aatcttgtaa aaccgtcatc ttccatgtag tcgataatat tttgttttaa
      601 ccaaatcatt tcttcatgtg tgagtttatt gggattaaat tcaacacgca tattacgtct
      661 atcccaagta tctgctttta ctttgtcata ttcgatataa actttttctt gaagtgcctt
      721 agctttaaac tttgtttgaa gtatatccca aagtcgtatt tgtggctcta cactcataaa
      781 gtcagatagc tttttagcat tagttttgtt caaatttcca acgattgtca tggcatcaaa
      841 acttaatgcg ggttgagatt ttcccaaagt ttgaccactt aaccggctat tacttaaccg
      901 gctattagag acggccggtt tttttagtga gatttctgca gctaattttt catcaatttt
      961 cttcattttt tatcgctcct aaaataattt ttggaacgaa aaaaggacga gtggaatcgc
     1021 aaaataaatt tcgcctgtat ccattcgtcc attttgtttt gtattcaaaa tcaaaaattg
     1081 atatactaat catcgaatga ataacaagtg tggtttgtta ttccgggcga gaacgttggt
     1141 agcgttgctc gtcttttttt atgtcttttt ttcgttaaaa ctattttaaa actaaatata
     1201 tcttaaagca aataaattca aggggctaaa agcgcccctt aaaaacccct tgcctctgaa
     1261 aagttttgtg tttcctttcc gtcggtttca aaagctaatt tactcaaaaa aatagatcaa
     1321 gggtaaatgc actcactgcg ttcgcccttg agctatttta tttcgcaaat tttaacgcat
     1381 ttgccgacga aaagataaaa cacataaaaa tatacgcaga tatatttaaa acgtcttaga
     1441 acgcaaaatt ttacgtttaa aagctatgca aggactgtat gaagcaaaat tattgagtta
     1501 tccaagaaca gatacaccat ttattacaga gaacgaattt gcttatttaa aagcgaattt
     1561 tggcaaatat agcggttttt taggacttga tcttgaaatg gttcaaacag agcctagaaa
     1621 gcgttatgtg gacggtagta aggtacagga acaccacgcc attatcccaa caaaacaagt
     1681 acctaccgaa tctgcattag cgaaaatgga cgatttacaa cgaaaaattt atgctttagt
     1741 cgttaaaacg accgttgcca tgtttttacc tgattacttg tatgaagaaa ccaagataca
     1801 aacgaaagta gctgatttgc tgtttcaatc gatcggaaag acaccaaagc aagaagggtg
     1861 gaaaattctt ttcaaacaac aaaccaaaga agaaaaagag gacgttcaaa cgttaccact
     1921 cgttatcatt ggcgaacgtg ccgaagttgg tgttaagagt gttgaaaaag aaacgcaacc
     1981 gccaaaagct tttacagagg gtacattatt aactgctatg aaaacggcga ataaaacggt
     2041 tgatgatgaa gaagcaatca agattttaca agaagttgag gggattggaa cagaagcgac
     2101 aagagcaagt attattgaag cgttaaaaca aaaagaatat atccaagtga ttaagaataa
     2161 gcttgttgta actgaaaaag gaaaattatt gtgccaggca gttgaaagtc agcacctttt
     2221 aacgagtgct gaaatgacgg ctaaatggga aacgtattta aaaaaaatcg gtaaaagaga
     2281 aggcaatcaa gagaacttta ttacgaatat caaaaaattc attgttcatt tactggaagc
     2341 tgtacctaac gatatagaaa aactaaattt ttctgattac caggaacaga aagaaaaaga
     2401 agcagaaaaa agtattgtag gaaaatgtcc taagtgtggc aacaatattg tattaaaaaa
     2461 atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg aacattttag
     2521 aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag aaaccctggt
     2581 aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa ttggggaaaa
     2641 gggatatatt gattttatat ctttctcaaa ataaacataa aagcccttta aagagggctt
     2701 ttatatatta atcacaaatc acttatcaca aatcacttat cacaaatcac aagtgatttg
     2761 tgattgttga tgataaaata agaataagaa gaaatagaaa gaagtgagtg attgtgggaa
     2821 atttaggcgc acaaaaagaa aaacgaaatg atacaccaat cagtgcaaaa aaagatataa
     2881 tgggagataa gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag
     2941 caaagaatgg cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga
     3001 gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt taaattagat
     3061 gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgtaatg
     3121 tagataaaac atctactgtt ttgaaaca
//
LOCUS       DAZKMZ010000160         3111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000160 DAZKMZ010000000
VERSION     DAZKMZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.165"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8M5_002749"
     CDS             278..787
                     /locus_tag="ACG8M5_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0359933.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8M5_002750"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8M5_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0359934.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..>3111
                     /locus_tag="ACG8M5_002751"
     CDS             2322..>3111
                     /locus_tag="ACG8M5_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0359935.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPK"
BASE COUNT         1097 a          504 c          620 g          890 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa g
//
LOCUS       DAZKMZ010000161         3102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000161 DAZKMZ010000000
VERSION     DAZKMZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.166"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            776..>3102
                     /gene="esp"
                     /locus_tag="ACG8M5_002752"
     CDS             776..>3102
                     /gene="esp"
                     /locus_tag="ACG8M5_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0359936.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSYLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1133 a          460 c          601 g          908 t
ORIGIN      
        1 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
       61 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
      121 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
      181 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
      241 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
      301 ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt tataattata
      361 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
      421 gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt
      481 aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
      541 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
      601 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct
      661 ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca
      721 gatttatctt ttattcttac taggaaagta attattaagg gagttgattt gaataatggt
      781 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
      841 tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg
      901 aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc
      961 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
     1021 tggttatacg aaaaattata acttttcgta tttacaattt agtcctcaag aactaactgg
     1081 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
     1141 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
     1201 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
     1261 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
     1321 tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac
     1381 gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga
     1441 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg
     1501 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
     1561 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc
     1621 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
     1681 tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg
     1741 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
     1801 catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc
     1861 tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa
     1921 taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg
     1981 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
     2041 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
     2101 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
     2161 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
     2221 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
     2281 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
     2341 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
     2401 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
     2461 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
     2521 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
     2581 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
     2641 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
     2701 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
     2761 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
     2821 accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata ataagtactt
     2881 cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt
     2941 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
     3001 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
     3061 aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DAZKMZ010000162         3081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000162 DAZKMZ010000000
VERSION     DAZKMZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.167"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(511..795)
                     /locus_tag="ACG8M5_002753"
     CDS             complement(511..795)
                     /locus_tag="ACG8M5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0359937.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(1314..1799)
                     /gene="vanZ-A"
                     /locus_tag="ACG8M5_002754"
     CDS             complement(1314..1799)
                     /gene="vanZ-A"
                     /locus_tag="ACG8M5_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0359938.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1952..2863)
                     /locus_tag="ACG8M5_002755"
     CDS             complement(1952..2863)
                     /locus_tag="ACG8M5_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0359939.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          933 a          568 c          435 g         1145 t
ORIGIN      
        1 aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac tctttcttaa
       61 acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta ttttttatat
      121 aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa tacaaaaaaa
      181 gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag cttagatact
      241 tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc ttcaaacaaa
      301 gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata agcgttggtt
      361 tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat aaatgtgtca
      421 tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat accgctttta
      481 aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt tttcttcttc
      541 catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt ctgttggctc
      601 atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt gttctagtgt
      661 ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc tactcatttc
      721 taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta agctttctct
      781 aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac gctttattta
      841 aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt tgtgattatt
      901 ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat tattggactg
      961 gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg taattctctt
     1021 taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac cgtcaggaaa
     1081 atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag cactaaaatt
     1141 gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt atcaccggtt
     1201 ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca tctaatccaa
     1261 ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac atcttacacg
     1321 taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag tatacctaca
     1381 aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa accatataat
     1441 ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc tgtcgctcca
     1501 atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac aaaagcaaac
     1561 ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa tggaataaag
     1621 attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg agtcaagtta
     1681 agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt gaataacact
     1741 aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat ttttcccaat
     1801 ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg tcctagttaa
     1861 cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa taatgctgtt
     1921 cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta tgtgttgatc
     1981 cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca taacgaagat
     2041 tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc tcatattttt
     2101 ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata tattcctcga
     2161 gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag cgaatatgcc
     2221 atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt aaaatgaacc
     2281 cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc attttcgtca
     2341 agcttgatcc tacatctagt gataaacctg aattatgctc actataacct gctggtaagg
     2401 catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct cgatagccac
     2461 tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc tctgaaaatt
     2521 tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat ccatttatta
     2581 attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg cgaacaggat
     2641 atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta attgttttct
     2701 cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt tgatcataat
     2761 tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca taacctaagt
     2821 atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt ctcctcctta
     2881 actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg cacaatctcg
     2941 tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc tactggattt
     3001 actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa tttaacgagg
     3061 ctgaactgcc tgttgacaat g
//
LOCUS       DAZKMZ010000163         2989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000163 DAZKMZ010000000
VERSION     DAZKMZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.168"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1727
                     /gene="alsS"
                     /locus_tag="ACG8M5_002756"
     CDS             63..1727
                     /gene="alsS"
                     /locus_tag="ACG8M5_002756"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0359940.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1740..2450
                     /gene="budA"
                     /locus_tag="ACG8M5_002757"
     CDS             1740..2450
                     /gene="budA"
                     /locus_tag="ACG8M5_002757"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0359941.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2580..>2989
                     /locus_tag="ACG8M5_002758"
     CDS             2580..>2989
                     /locus_tag="ACG8M5_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0359942.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGY"
BASE COUNT          993 a          500 c          683 g          813 t
ORIGIN      
        1 caattatttg tgattatttc taactaataa ttaaaaataa agcataaaaa ggacgatgtt
       61 caatggacga aaaaatgaaa caaggttcga aatctgtagt agaaagtttg attaatcatc
      121 atgttgatta tgtattcggt ataccaggtg caaaaatcga tggtgtattt aatgaattag
      181 aggatcaagg tcctgaatta atcgtgactc gtcacgaaca aaatgctgca tttatggcac
      241 aagcaatcgg acgtattaca ggagagccag gagtagttat cgcaacaagt ggtcctggtg
      301 ctagtaactt agctacaggt ttggtaacag cgacagcaga aggtgaccca gttttagcaa
      361 ttgccggaca agtaaaacga agcgatctat tgaagttgac ccatcaaagc atggataatg
      421 cagcactgtt ccagccaatc acaaaataca gtgcagaaat ccaagatcca gaaacaattt
      481 cagaagtgat tgccaatgct tatcgtatgg ctaagagttc aaaaaaaggc gcgagcttta
      541 tcagtatccc gcaagatgta gtggatgcac ctgtccaagg gaatgtcatc aagccattga
      601 gcgatcctaa attaggttct gcttctgcag atgatatccg ttacctagct gaacgcatta
      661 gagaagcaaa actaccggta ctattagtag gtatgcgagg atcaagtgaa aaagaaacgt
      721 tagccatcag gcaattagtt ggaaagacag cattgcctgt tgtagagaca ttccaagcag
      781 ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg tcgtgtgggt ttatttagaa
      841 accaaccagg agatatgctt ttgaaacgaa gtgatctagt tattgctatt ggctatgatc
      901 caatcgaata tgaagcaaga aactggaatg cggaaaaaga tgcgcggatc attgtgatcg
      961 atgaagcgcc agctgaaatc gatcctttta tgcagcctga aagagaattg atcggtgata
     1021 tctcagccac tcttgattta ttgacaggtt cattagagcc tcaacaagtg tcagaagatg
     1081 caaaagaata tttagcatct cttcaagcag aattaacgga gcgggacatt gttcaatcaa
     1141 aaggagaagc aggtatcttg catcctttag aagttattaa cacgcttcag tcaaaagtaa
     1201 cagatgatat gactgttacc gtagatgtag gtagtcatta tatttggatg gcacgccact
     1261 ttagaagtta tgaacctcgt catttgttat tcagtaatgg tatgcaaacg cttggtgttg
     1321 cattgccatg ggcgatttct gctgcattgg tgcgtccaaa tacacaaatc gtatcagttt
     1381 ccggcgatgg gggattttta ttctctgccc aggatttaga aacggcggta cgtaaaaaat
     1441 tgaatatcgt tcatttgatt tggaatgatg gacactacaa tatggtagag ttccaagaaa
     1501 aaatgaaata tcagcgggcg tcaggtgtag actttggtcc agttgatttt gtgaaatatg
     1561 cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt agaggaatta gaaaaagctt
     1621 tagaagaagg atttgcgaca gaaggacccg taattattga tattccgatt gattaccgtg
     1681 ataatgagaa attgggagaa acaatcttac cagatcaatt ctattaagga agtgtcgaca
     1741 tgactgaaaa aatcttatac cagcacggga cattaggagc tttgatggct ggcttgatgg
     1801 atggaacaga aacgattgct catatattag aaaaaggaac tctgggattg ggaacattgc
     1861 atgggcttga tggtgaagtc atctttttag atggcgtagc ttatcaagga agatcagatg
     1921 gctcagttgc ccaattagat ggaagtgaac ttacccccta tgcagcaatt acagatttta
     1981 cgccagatac tagtttttct gtctcaaaaa ctgcagatgg agaacaattg aaaaaagaca
     2041 tattagtaag agaagccggg gaaaacctat ttttggcagt gaagataact ggattattca
     2101 aaaatatgca catccgtatc atgccgaagc aagaaaaacc ttacagaaga ctagcaaaaa
     2161 tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca aggaacatta gtcgggttct
     2221 ttacgcctga attattccaa ggagttgctg cagcgggatt tcacttgcat tttatcgatg
     2281 atacacatac atttggcgga catgtaatgg attttgaagt ggctgaagga aaagttgaga
     2341 ttagccgcat ctcgtccctt gtacagcatt ttccagttga tgatccgact tttgttcacg
     2401 aaacgattga ttatgctgat ttagcagcag aaatccaaca agcagaatag aaaaagacag
     2461 cgagatgcgt tcaaggtgat taacctgacg tatctcgttt ttttgaggag gaaacctagc
     2521 gtttacaaaa aaactgagct ataatcttct ccatagatca aaaaggagcg gtttttatca
     2581 tgtggacaga aaaacagaaa aaagatgcat acgaaatttt attattacag caacaaggat
     2641 tattagaagc agaagataac tggttagcaa atttagcaaa ttcatctgca ttgttgaatg
     2701 agactttgcc tgagactgtc tttgcaggat attatttatt cgatggtaat gaattgattt
     2761 taggaccgtt ccaagggcga gtttcttgca cacgtatcaa gatgggaaaa ggagtttgtg
     2821 gagaatcagc tgaaaaacaa gaaacattga tcgttgatga tgtcaaaacc catgcaaatt
     2881 acatttcctg cgattcagcc gcacaatcag aaatcgtagt gccaatggta aaagaaggta
     2941 atttaatcgg tgttttagat atcgattgcg gagtaacgaa agggtatga
//
LOCUS       DAZKMZ010000164         2948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000164 DAZKMZ010000000
VERSION     DAZKMZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.169"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8M5_002759"
     CDS             <1..189
                     /locus_tag="ACG8M5_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0359943.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ACG8M5_002760"
     CDS             complement(275..1369)
                     /locus_tag="ACG8M5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0359944.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="ACG8M5_002761"
     CDS             1558..2319
                     /locus_tag="ACG8M5_002761"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0359945.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8M5_002762"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="ACG8M5_002762"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0359946.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          944 a          583 c          496 g          925 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatattt
//
LOCUS       DAZKMZ010000165         2934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000165 DAZKMZ010000000
VERSION     DAZKMZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.170"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..1821
                     /locus_tag="ACG8M5_002763"
     CDS             190..1821
                     /locus_tag="ACG8M5_002763"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0359947.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1824..2903
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002764"
     CDS             1824..2903
                     /gene="uxuA"
                     /locus_tag="ACG8M5_002764"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0359948.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
BASE COUNT         1025 a          396 c          612 g          901 t
ORIGIN      
        1 agaaaaaata agtttcgata aaatcagtcc aaaaagaagg agtattttat ttttctgaat
       61 aaaaaataca atcattctga tatggtagaa aaaagcaagt ttattccctt tttatattga
      121 caacggttac aatgggtttt aaaatataaa atgtatcaaa actaacatat cagtttgagg
      181 aggttaatta tggtttcgat taaaaattgg caaaattcta tcgaagaatt caatgaaatg
      241 cagattctac tgccgtgttt tgatgtcaaa gaagttaagc aagaaggcca gcaacatcca
      301 gtatggcttc attttggtgg aggaaatctt tatcgaggat ttcatgcaga aattgctcaa
      361 tgcttagcag atcaaaatga attagaaaca ggagtagttg tgtgtgaaac atttgatgaa
      421 gaagtagtgg aaaaagctta tcatgactat aacaatgaca ttttggaaat tgttatgagt
      481 ggagatggtg aactcaaaaa aagacttttg gcagtaacgg ctgatagttt atattgcaat
      541 ccgaagaata tagcggacta tcaaagagtg gttaaatatt ttgaagatcc taaattacag
      601 tttacaacgt ttactattac tgaaaaaggt tacagtttaa aagattctaa agggcaattc
      661 ttttcggtag tcaaagctga tatcaaatct ggacctagca atcctcaaca tacgatgagt
      721 attgtaacat ccttgttata tagcagattt aatgctggag agttgccgat tgcgatggtt
      781 tctactgata atttctcaca gaatggtcaa cgttttcaag atagtatttt tacgattgca
      841 gaagcttggc aaaaagaagg atatgtttct gaaaaattta ttgcttattt aaaggataaa
      901 gaaaaagtga cttttccttg gagtatgatt gatcgaatta cgccaaatcc ttcagagaat
      961 gtaaaggaag atctattgat gatggggctc gaggatttag atatcattca cacaaaaaaa
     1021 cataccaata ttgctgcttt cactaatact gaggtagttc attatttagt tattgaagat
     1081 agttttccaa atggccggcc taaattggaa aaggctggtg tgattatgac agatagagag
     1141 acggttgata aagcagacat catgaaggtt acgacaagct tgaatccact tcatactgct
     1201 ttggcagtaa caggttgtct gctaggatat caatcaattt cagcagagat gcaggatgat
     1261 gatttggttg gtttaatcaa aggaataggt tatttagaag gtttaccagt agttgaaaat
     1321 ccaggcatta ttgatccaaa acaatttatt gatgaagtaa ttaataaacg tttgcctaat
     1381 ccctatattc cagatacacc acaacgaatt gcaacagata cttctcaaaa agtagcgatt
     1441 cgctatggtg aaacaattaa aaagtatgtc gaaaaagacg gaaatgcaga aaatctaaca
     1501 tttattccta tcgctattgc tgcttggtta cgttatttac tggcagtcga tgatcaaggg
     1561 gaaaattttg tgccaagtcc tgatccgtta ttggtagagt tgcaggataa attatcagta
     1621 ttgaaattag gtgaacaaga tccttcaaag attcattgtg cggtacagga aattttagaa
     1681 aataaagaaa tctttggttt agatttatat caagttgggg taggaaagaa aactgaaaaa
     1741 atcttttgcg aaatgctagt aggtcctcaa gcagttcgta aaacaatcaa taaatatata
     1801 acagaagttg gaggaaagta actatggaaa tgacttttag atggtatggg gcaaatgaag
     1861 aaaaaatctc actacaagaa attcgtcaga tcccagcaat gaaaggaatt gttggaacat
     1921 taatggatat accagccgga gaggtttggc cgagagaacg aattaaatct ttaaaagatg
     1981 aagtcgaagc caatggactt accttgaaag ttattgagag tgttaatgtt catgatgata
     2041 tcaaaattgg tttaccaaca cgagatcaat acattgagaa ttataaacag acgattcgca
     2101 atttggcaga atttgggatt gaggttatct gttataattt tatgccagta tttgattggg
     2161 taaaatcgga tttggattat cgtttagatg atgggtcttc tacgttagcc tatatttcaa
     2221 aaaatatccc tactgatccg gaggaaatta ttagagaagt agaaaattct actaataatt
     2281 ttgaattgcc gggttgggaa cctcatcggt tatcacaaat taagcaatta ttagaagcct
     2341 ataaagaagt tgatgaagag aaattacgag agaactttgc atattttctc aaagcaatta
     2401 tcccgacatg tgaagaagtt gggattaaaa tggcagttca cccagatgat ccaccatatt
     2461 ccattttcgg attacctcgt attgttaaaa atcgtgacga tttagattgg ttgtgtaatg
     2521 tagtggattc tccttctaat ggtattacat tatgtactgg aagtattgca gaacgaccag
     2581 ataatgatgt ctatgaaatt ctatctgaat tttgtcaacg ggatcggatt ccttttgcac
     2641 atgttcgtaa tatcaaatat ttaagtgaca atggactcga tttttacgaa gcgccgcata
     2701 aatcagaata tggttctcta gatatgtata agatattaaa aacaatgtac gataatggat
     2761 ttaatggcta tattcgtcca gatcatggtc ggatgatttg gggagaaacc ggtagacctg
     2821 gttatggatt atatgaccga gctctaggtg cagtttattt aaatggtatt tgggaagcgc
     2881 ttgaaaaggg ggcgagtaaa taattatata agggagttta tttagaggaa aaat
//
LOCUS       DAZKMZ010000166         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000166 DAZKMZ010000000
VERSION     DAZKMZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.171"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8M5_002765"
     CDS             85..981
                     /locus_tag="ACG8M5_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0359949.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8M5_002766"
     CDS             1073..1288
                     /locus_tag="ACG8M5_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0359950.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8M5_002767"
     CDS             1306..1578
                     /locus_tag="ACG8M5_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0359951.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8M5_002768"
     CDS             1580..2443
                     /locus_tag="ACG8M5_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0359952.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKMZ010000167         2920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000167 DAZKMZ010000000
VERSION     DAZKMZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.172"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..951
                     /locus_tag="ACG8M5_002769"
     CDS             136..951
                     /locus_tag="ACG8M5_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359953.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1077..1334
                     /locus_tag="ACG8M5_002770"
     CDS             1077..1334
                     /locus_tag="ACG8M5_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359954.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1586..2158)
                     /locus_tag="ACG8M5_002771"
     CDS             complement(1586..2158)
                     /locus_tag="ACG8M5_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0359955.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2174..2779)
                     /gene="fic"
                     /locus_tag="ACG8M5_002772"
     CDS             complement(2174..2779)
                     /gene="fic"
                     /locus_tag="ACG8M5_002772"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0359956.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          956 a          529 c          455 g          980 t
ORIGIN      
        1 agtgactaga gaattaaata aaaaataatt actatctagt acgaaattaa ttacatagga
       61 taatatatta aggtgctgga ctacaagtaa aaaatactgt atgatcatga cagattcaag
      121 aaaggagcaa taagattggt taagatagca ggactttcta aaggtggaaa aaccgtttca
      181 caactttata aacaaaacta cagagactgg agacagttca gaaaaaacat taaggattct
      241 tactttattc taccaacaga acttgaaaaa tatttaccga ataacaaagc tattaattta
      301 tatctgtatt actgttttaa ggcaaagaat gaaggcgggg attcctggca ttcggtcaat
      361 accatagcag agtctctagg agtgacaact aaaactataa acacctggaa taaaacctta
      421 gttgatttag gtatgattgc acgtatagac gataaccatc tcagtaaatc gacaatttta
      481 cttcctctct ctagttacta taaattagcc cttgatagtt ctttaaagga tgttaccgag
      541 agcactgtcc atgaaattga tggcaacttg gttgcagttt atcatttgtt tgaatggaga
      601 aaaaatgagg ctgatgaaaa ttataacgtt ccttataaca cactctgttg tgtttttaaa
      661 agaaaaacac aaaaagatac tatctataaa tttatcttgg tccaagatga gaaaataagt
      721 aatttcgaat taagcactcc ttctagtaag atttatgacg atgcatatag atttgaatgt
      781 caagatctag aagccctcct ttccgcaaat aatctaaaca atatagaaca tgaaaacttt
      841 gttgtaaaca ctagatttaa tttagtttct gaaaaggact cagatttgtt agatttgctg
      901 atagagctta ctaaaaatct agataagaaa gaaaacatta ctgtactata actagcatcg
      961 gtccatcatt aagtttggac tattagaaca cttgctatta cagaaaaagg tatgctagcc
     1021 acgcttacta tttattggta tcaaacaagt atcttaattt gaaaggaaga tgatttatgg
     1081 ctaatttata tatagagaac aaaggagact tgaatgccat tattacttta atcgtagtta
     1141 tggtaaaatc aataggtaat atcacaatag gaatgctgtt tattatatta gcagcagtgc
     1201 tgatttttat gatagttatt tggttcttaa atgaaagatc taaagacaat caacaccgta
     1261 gggatatgga gataaaatca atagagtcaa tagattattt atcgataata acactaaaaa
     1321 agccacgttc ctaaacgtga cttacgctga acgatatgtg ttgtttggta gacgaacaaa
     1381 tcgttcatta taaatatatg atggtatcct aatatagtaa gcgtggctag cataccttta
     1441 attactttac cttttacagt taccatacac attttttgag cagaaatcaa gtaaataaaa
     1501 aaatattata tttcacaaca tgctaataga ctcatatcta gaacgcatta agtatttacc
     1561 tgtctggttc gctgagactt cttttttaac tttcttcaat tttcttcttt attctaaaaa
     1621 gcgtactgcg actgaaaccg gttatagctt caacttcctt atacgattta cctgaagtaa
     1681 gcagttcata tgcgtgcctc ttcttcggtg tgattgttgc tttcggacgt ccttctctga
     1741 aattaggatt attctttctt gcaaacattt ttccttcttg ggtacgttcc acaatcatat
     1801 cacgttccat ttctgctaca ctcaacaacg tccgtgtcac cattcgcccc atggacgtat
     1861 tttcaatcgt tcctagattc aaaaccttga tcgttacgtt atcatcgagt aaaggttcaa
     1921 tgatctctaa tgcttctctc gtgttacgag caaatcgatc aagcttcgta acaactattt
     1981 catctccagg cttcagacca gccaacagct gattgaattt tggccgatcc cgttttgtac
     2041 ccgtaaattt ttctgaaaaa atattcttct tctctacgcc gttctcaagc aactgttgaa
     2101 tttgttcttt tgtactttga gaagcggttg agacacgtgc atatccatat ttcgtcattt
     2161 ggtccaccta cttttacaaa tcatctatcg tatactcaga atacccttca tagaaatagc
     2221 tgacatcgat tcctttcata tataactcgc gatcgtcaat tttatcagtc agagcattgg
     2281 aaattagata gcgtatttcc aaatcgttta tgggacttcg ctccattgca gaaagatagt
     2341 ctgctttatc gattaagttc caatctacaa ctttttgcaa ttcttccttt aaaatgagat
     2401 ccagccaaat tctagtactt cggccatttc cttctcgaaa tgggtgagcg atattcatct
     2461 ccacatattt cttgataatc tcttcaaaag aagtttgtgg catctgatca atatgctgta
     2521 acgaatgttc taaatacata actggtgcaa atcgaaaatt tccttttgca atattgaccg
     2581 atcggatttt tccggcaaaa tcgtaaatat ccgaaaatag aaattcatga atttcagata
     2641 aacctttaaa tgtaccaact tcgatttcat ttatttttcc acaatcgtac aattctttcg
     2701 ctttttgttt actgagtttt tcttcaacct tagccaactc aacttgattg gttaatccca
     2761 atttattctc taaaatcatg cgaaatctcc ttaactttat gacataagtt tatgaaacac
     2821 tctcaacccc attatatcgc gattttgttt atgtgtcaaa aactattaag tttctgaccc
     2881 ccgatagatt tttcaagtgt ctctattttg cacagtctag
//
LOCUS       DAZKMZ010000168         2771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000168 DAZKMZ010000000
VERSION     DAZKMZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.174"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..410)
                     /locus_tag="ACG8M5_002773"
     CDS             complement(<1..410)
                     /locus_tag="ACG8M5_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFQ0359957.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQP"
     gene            complement(415..600)
                     /locus_tag="ACG8M5_002774"
     CDS             complement(415..600)
                     /locus_tag="ACG8M5_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0359958.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(954..1199)
                     /locus_tag="ACG8M5_002775"
     CDS             complement(954..1199)
                     /locus_tag="ACG8M5_002775"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359959.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1193..1594)
                     /locus_tag="ACG8M5_002776"
     CDS             complement(1193..1594)
                     /locus_tag="ACG8M5_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0359960.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1781..>2771)
                     /gene="uidA"
                     /locus_tag="ACG8M5_002777"
     CDS             complement(1781..>2771)
                     /gene="uidA"
                     /locus_tag="ACG8M5_002777"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0359961.1"
                     /translation="FGIRSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNI
                     KDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNV
                     EKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVEL
                     TKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKE
                     LDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVG
                     EQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          875 a          537 c          352 g         1007 t
ORIGIN      
        1 aatggttgct caatgccctt ttttacaaca tggattttcg ataaaaactg ttgaaattcc
       61 tgtatatgaa ttggtcgaat ctttacaacc tgcatttctc caaaaaaatc caatgcacgt
      121 tgtaaaatat gatctgtttt catatatgtt ttttctacag ttcccaactt ataccaagga
      181 aaaaaatact ctcgataaaa atcatgaaac gttattcctt gcagataaat tccttgtgaa
      241 tcagaaaaac tttgttttaa ctcttcgagt ccccttctag cttctccttg agttttgaag
      301 cctcttctgg tcacttgaat tctttttcca aagcgatctt gtcctaaagt tgcagaaaaa
      361 aaccaagtgc ctctttttct gtctttataa acattcgcaa ttttcctcat tttactattc
      421 ctcccaagaa atatggagaa cttcttctaa aattgaaata ggaattcttt ttatttttga
      481 attacgataa aaaaagtatc ctttctcaac taaaagttct ttacattcct tgaagatttt
      541 attagcatag tactgagaaa aacctagatt aatcaaatct tttcgagtag ctgttttcat
      601 tatagcacct tccttacttt ttttcattta tttctatcag ctgcttttgt caaaagcaca
      661 taggaatttc acctctcccc aagtttacgg cgagccttac ggaagtatca ttatcccaac
      721 tttaaatcat cgctttttat tgactataaa aattatttta gaatatttat cgctccttct
      781 ttacagcaag aaatcatggc gcaactaaaa tatgctcctt aaagtgttaa cgtactgata
      841 gaatctattt aattttcaat gaacaatagg ttttctacct aaaagaatct actaattcct
      901 taactattag aagattcttt taaacagaaa aagtggaaag gggatttttt taattagtta
      961 attttaaatt ttaagatcgc ttctattaat ttaatttcta gctgacttat catatattca
     1021 tctacatagg taacgaattg acctacctcg ttaaatccat ttgtcaaaca taacttagcc
     1081 atataagaat gataatgttt gagtatcatc ttgatcgaaa ttggatctcc ttctagagca
     1141 gataagataa tggatagtgg aactaatagg tactgccgct ttttagattt atctaccata
     1201 ggtcataccc cattttcaat tgttcaactg ccttctttcg ttgataccag ataccacttg
     1261 tgctcatatt gagaatttta ccgatttcag catcattaaa acctgcaaaa taataaagta
     1321 ggataatctt tcttcttatc tccgataact gatcaattgc tgctgataac ttttgatctg
     1381 aaattaatat ttccattcca aataagaaat agacatcttg ttcatcaaaa tgagtttggg
     1441 aatacctgcc ttcatttgaa tctgccaatg gttcctcatt atcctttatg ttagcgtttc
     1501 gttttcgaat agacttagct tcgtttcgta atactttttt acagtaggca tcaaattggt
     1561 gttgtataga tatgtcattg taatgttgtt gcatgataat atgctccttt ttcccccttt
     1621 ccactaatac aacaatcaga aagccccttt ttttcgaaaa aactagaaaa aaaacataaa
     1681 aatatttatc attctttaaa cagaaaatct ttctataaaa aacaaaaaaa aacgattagg
     1741 ctaggctaat cgttttttga acattcttaa aaaatccaaa ttatttttta taactgaaat
     1801 tgggtattga tgaccatcgt ttctgtagat agtaagcaat cattttaggt tttctatctc
     1861 tagtaaagat tcctttctta tttccttgca ctcttaaaat cccttgactt gttgcaaaat
     1921 ctgcaaaatt ccacacttgt tcaccaacaa aattttcaca tgtatctatt acttcgtgat
     1981 ttgcttcgta ataagccact tgatactctt ctgtaaacat tgttggtact gtatcatgaa
     2041 atccagctac tgtatctgct ccatattctg taaacattat tggcgtatca ggacatcttt
     2101 ccatccatcc atctagctct tttttcaaca gatattttgc atgtgccaga tcccctcccg
     2161 aaacatacca accataataa cgatttaaac ataaaacatc gacaatatct cctaccttac
     2221 aaacatctgg cgttgatttt agataagtaa cgattgttac aggtcttttc tgcggatcaa
     2281 gttctttagt taattcaact aaaggttcaa aatattcttt agcaccttcg gaatccgaat
     2341 ctggttcatt tgcaatagac cacattacaa cacttgggtg gttcttgtcg cgttctatca
     2401 attccttgat caccgtttta tgagaatttt ctgttcgtat ctctttccac gtattatgtt
     2461 tttccacatt atcatctaat aaagtggcca tgaaatttaa atgtaaccct actgcaggta
     2521 cttcatcaat aactacaata ccttctcgat ctgctaatcg cataatttct tctgagtaag
     2581 gataatgtga tgttctaaaa gaattagcac ctatccattt cattaaatta aaatctttaa
     2641 tatttacagc ctcattcaaa ccacgtccat taacatacga atcctcatgc ttaccaaatc
     2701 ctttaaaata aaaaggttta ttattaatca aaaatttgcc attggtaacc tgaacactgc
     2761 gaataccaaa t
//
LOCUS       DAZKMZ010000169         2742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000169 DAZKMZ010000000
VERSION     DAZKMZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.175"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1046
                     /locus_tag="ACG8M5_002778"
     CDS             78..1046
                     /locus_tag="ACG8M5_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0359962.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1039..2070
                     /gene="vanA"
                     /locus_tag="ACG8M5_002779"
     CDS             1039..2070
                     /gene="vanA"
                     /locus_tag="ACG8M5_002779"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0359963.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2076..2684
                     /locus_tag="ACG8M5_002780"
     CDS             2076..2684
                     /locus_tag="ACG8M5_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0359964.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          811 a          528 c          679 g          724 t
ORIGIN      
        1 agagaaagat ttgacaagat taccattggc atccccgttt tatttggtgc ctttcacaga
       61 aagggttggt cttaattatg aataacatcg gcattactgt ttatggatgt gagcaggatg
      121 aggcagatgc attccatgct ctttcgcctc gctttggcgt tatggcaacg ataattaacg
      181 ccaacgtgtc ggaatccaac gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac
      241 ataaatcaga gatttccgcc tctattcttc ttgcgctgaa gagagccggt gtgaaatata
      301 tttctacccg aagcatcggc tgcaatcata tagatacaac tgctgctaag agaatgggca
      361 tcactgtcga caatgtggcg tactcgccgg atagcgttgc cgattatact atgatgctaa
      421 ttcttatggc agtacgcaac gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca
      481 ggttggacag cgaccgtggc aaggtactca gcgacatgac agttggtgtg gtgggaacgg
      541 gccagatagg caaagcggtt attgagcggc tgcgaggatt tggatgtaaa gtgttggctt
      601 atagtcgcag ccgaagtata gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata
      661 gcgatatcgt tacgcttcat gtgccgctca atacggatac gcactatatt atcagccacg
      721 aacaaataca gagaatgaag caaggagcat ttcttatcaa tactgggcgc ggtccacttg
      781 tagataccta tgagttggtt aaagcattag aaaacgggaa actgggcggt gccgcattgg
      841 atgtattgga aggagaggaa gagtttttct actctgattg cacccaaaaa ccaattgata
      901 atcaattttt acttaaactt caaagaatgc ctaacgtgat aatcacaccg catacggcct
      961 attataccga gcaagcgttg cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt
     1021 ttgaaaggag acaggagcat gaatagaata aaagttgcaa tactgtttgg gggttgctca
     1081 gaggagcatg acgtatcggt aaaatctgca atagagatag ccgctaacat taataaagaa
     1141 aaatacgagc cgttatacat tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa
     1201 ccttgcgcgg aatgggaaaa cgacaattgc tattcagctg tactctcgcc ggataaaaaa
     1261 atgcacggat tacttgttaa aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca
     1321 ttttcagctt tgcatggcaa gtcaggtgaa gatggatcca tacaaggtct gtttgaattg
     1381 tccggtatcc cttttgtagg ctgcgatatt caaagctcag caatttgtat ggacaaatcg
     1441 ttgacataca tcgttgcgaa aaatgctggg atagctactc ccgccttttg ggttattaat
     1501 aaagatgata ggccggtggc agctacgttt acctatcctg tttttgttaa gccggcgcgt
     1561 tcaggctcat ccttcggtgt gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt
     1621 gaatcggcaa gacaatatga cagcaaaatc ttaattgagc aggctgtttc gggctgtgag
     1681 gtcggttgtg cggtattggg aaacagtgcc gcgttagttg ttggcgaggt ggaccaaatc
     1741 aggctgcagt acggaatctt tcgtattcat caggaagtcg agccggaaaa aggctctgaa
     1801 aacgcagtta taaccgttcc cgcagacctt tcagcagagg agcgaggacg gatacaggaa
     1861 acggcaaaaa aaatatataa agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt
     1921 ttacaagata acggccgcat tgtactgaac gaagtcaata ctctgcccgg tttcacgtca
     1981 tacagtcgtt atccccgtat gatggccgct gcaggtattg cacttcccga actgattgac
     2041 cgcttgatcg tattagcgtt aaaggggtga taagcatgga aataggattt acttttttag
     2101 atgaaatagt acacggtgtt cgttgggacg ctaaatatgc cacttgggat aatttcaccg
     2161 gaaaaccggt tgacggttat gaagtaaatc gcattgtagg gacatacgag ttggctgaat
     2221 cgcttttgaa ggcaaaagaa ctggctgcta cccaagggta cggattgctt ctatgggacg
     2281 gttaccgtcc taagcgtgct gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata
     2341 acctgacaaa ggaaagttat tatcccaata ttgaccgaac tgagatgatt tcaaaaggat
     2401 acgtggcttc aaaatcaagc catagccgcg gcagtgccat tgatcttacg ctttatcgat
     2461 tagacacggg tgagcttgta ccaatgggga gccgatttga ttttatggat gaacgctctc
     2521 atcatgcggc aaatggaata tcatgcaatg aagcgcaaaa tcgcagacgt ttgcgctcca
     2581 tcatggaaaa cagtgggttt gaagcatata gcctcgaatg gtggcactat gtattaagag
     2641 acgaaccata ccccaatagc tattttgatt tccccgttaa ataaactttt aaccgttgca
     2701 cggacaaacg gttctgttgc aaagttttaa ataaagaata aa
//
LOCUS       DAZKMZ010000170         2718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000170 DAZKMZ010000000
VERSION     DAZKMZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.176"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..453)
                     /locus_tag="ACG8M5_002781"
     CDS             complement(<1..453)
                     /locus_tag="ACG8M5_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0359965.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLS"
     gene            complement(573..1220)
                     /locus_tag="ACG8M5_002782"
     CDS             complement(573..1220)
                     /locus_tag="ACG8M5_002782"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0359966.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1304..>2718)
                     /gene="uxaC"
                     /locus_tag="ACG8M5_002783"
     CDS             complement(1304..>2718)
                     /gene="uxaC"
                     /locus_tag="ACG8M5_002783"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0359967.1"
                     /translation="NFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQYKNLT
                     QVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPIFEWSH
                     LELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDPTDDL
                     WYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGLKQALS
                     QRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTGIQ
                     LHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFDTA
                     EKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTT
                     MAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVE
                     NICYRNAYNYFNFFEIGSRK"
BASE COUNT          850 a          572 c          371 g          925 t
ORIGIN      
        1 cgatagcatt gttctttctt ctgtaataga agtaatagct gatgccattg atccataagg
       61 aatatttata gatgtgtaaa aaatactccc ccataatata tacgtaacat acatgtatac
      121 taccttaacg ctcatagaag ctgaaatcat agaagtttga tacattaaaa aacttgctaa
      181 agcgactgga ccactcattc ttaaaatcca cggtttaaac ttaccctgct tagttgcttt
      241 cgatctatca acaatacgcc ccatagttac atcagtaact gcatcgataa atcttgctac
      301 taaaaataat gttccaacgg cagctccgct tatccccaat acttttgtat aaaagatcat
      361 taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca aaatctccaa acatatatcc
      421 tattttgtct cttataccaa attttctttc catacaattc tctcccttct gttgttaagc
      481 gttttcattc tgatgaaagt acgtactata ctctgaagcg taaagagtca gtatttattt
      541 tttacatttc agagtattat ttgttttttc tcctaataat tctttatctc ataaaactta
      601 tctatatatt tactagctac tcgacttact tcttcaaaat cacctttttc aacagaagcc
      661 aataaattcc cacctacacc aactgcaaca actcctactt caaaccaatc ttctaagttc
      721 tctatactta ctccgccagt aggcattata tttacgtgtg gtaatggagc ttgataggct
      781 tttacaatgg atggaccaaa agcagaacct gggaatagtt taatgatatc tacgccagca
      841 gtaagtgcct cctttatctc agtaattgtc atacaacctg gtaaataagg aatttgatat
      901 aaattacaaa tctcagctgt ttgtcgatca aaagatggac taacgatata ttctgcccca
      961 gccataattg cgatacgagc agtaaacgca tctaaaaccg taccggctcc gattattaca
     1021 tctttttgat cagcatattt tttagcaatt tctttgatta tttcttcagc attaggaact
     1081 gtaaaagtta gttcaatccc tttaattcca cccgcaataa ttgaatttat tgctttaatg
     1141 gcctcatcct tatttttccc acgtacaacc gcaatgactc cagccgcttc taacttactt
     1201 aaaatttcta cttttttcat atctatctcc ttcctttatg gatacatact ttgtctagct
     1261 gtactaactt acaaagaata aatttaagct tttatttcaa gtattatttt ctacttccga
     1321 tttcaaaaaa attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac
     1381 taagatacat ttcatcatct ggaattcggc ctgctttaac ccactcgctt agtaattgac
     1441 ataatactcg acgaaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca
     1501 acatacctgt aaaattcgac aacaaacttt gttgtgccat cgtagtcaac tgttgacgca
     1561 ttccttcagc tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac
     1621 ccccttggaa gctttgcatt gctgttgcta gtggaagcca atcagttgga tttaatgaat
     1681 ataaaattgt ttttggtaag cacccactct tttcagcggt atcgaacaat gctacaacat
     1741 tttgtgtcaa actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt
     1801 cctttaatat acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat
     1861 atcttgtata ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg
     1921 ataaatggaa atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt
     1981 catctgctat ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt
     2041 gatgaaaatg atccactcgt tggctcaatg cttgcttcaa cccattaaag tctttgattg
     2101 gtatagccgc tgtttgacta agttttaaaa tatagttcga aaagcctgat gatttgatac
     2161 tccagacctt atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa
     2221 gctcagtatg ataccacaag tcatctgtag gatcatccgt tgtacagatg gctttcacat
     2281 ccatcatctc caacaattct ttcgggcgat aggaatccgt ttgaattttt tcgttagcac
     2341 gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa atcaatatca aagtaacgtt
     2401 gtaattccaa atgagaccat tcaaaaatag gattacctgg cgccttctcc actgttttta
     2461 caaaagctaa aaatttttca taatcatcac ctggaccact gatcaactct tcttctatac
     2521 cattagctcg catcaatcgc catttataat gatctccagc tcctttatca tttaaccata
     2581 cctgagtcaa attcttgtat tgcttatttt ctagcaaata tgctgggtct aaatgacaat
     2641 gatagtcgat aattggttgg tgttttgcaa atttatgaaa aagtctttta gctatttcat
     2701 tctctaataa aaagttct
//
LOCUS       DAZKMZ010000171         2704 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000171 DAZKMZ010000000
VERSION     DAZKMZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.177"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..187)
                     /locus_tag="ACG8M5_002784"
     CDS             complement(<1..187)
                     /locus_tag="ACG8M5_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0359968.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPM"
     gene            411..1211
                     /locus_tag="ACG8M5_002785"
     CDS             411..1211
                     /locus_tag="ACG8M5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0359969.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1230..>2704
                     /locus_tag="ACG8M5_002786"
     CDS             1230..>2704
                     /locus_tag="ACG8M5_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0359970.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQ"
BASE COUNT          998 a          416 c          529 g          761 t
ORIGIN      
        1 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
       61 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
      121 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
      181 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
      241 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
      301 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
      361 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
      421 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
      481 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
      541 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
      601 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
      661 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
      721 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
      781 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
      841 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
      901 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
      961 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     1021 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     1081 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     1141 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     1201 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     1261 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     1321 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     1381 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     1441 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     1501 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     1561 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     1621 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     1681 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     1741 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     1801 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     1861 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     1921 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     1981 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     2041 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     2101 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     2161 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     2221 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     2281 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     2341 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     2401 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     2461 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     2521 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     2581 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     2641 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     2701 aaga
//
LOCUS       DAZKMZ010000172         2641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000172 DAZKMZ010000000
VERSION     DAZKMZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.178"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..358
                     /locus_tag="ACG8M5_002787"
     CDS             <1..358
                     /locus_tag="ACG8M5_002787"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0359971.1"
                     /translation="IIVSSANKEAVQEEWERHELLSLVDELCCQDKGKKEDIIRSVIE
                     NGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKEKESWENLRTDVLFKCLSGDYVD
                     IEQKYIDTFWKNLTNK"
     gene            371..2137
                     /locus_tag="ACG8M5_002788"
     CDS             371..2137
                     /locus_tag="ACG8M5_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0359972.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            2160..>2641
                     /locus_tag="ACG8M5_002789"
     CDS             2160..>2641
                     /locus_tag="ACG8M5_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HFQ0359973.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     "
BASE COUNT          965 a          313 c          537 g          826 t
ORIGIN      
        1 aattattgta agctcagcta ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt
       61 attatcatta gttgatgaac tgtgttgtca agataaagga aaaaaagaag atattattcg
      121 aagtgtaatt gaaaatggtt gtgatttgga taagatatta atgattggtg attctcctgg
      181 tgatctagaa gctgcaaata aaaataaagt tttcttttat cctattttgg tcaataaaga
      241 aaaagaatca tgggaaaact taagaactga tgttttattt aaatgtttat cgggagatta
      301 tgtagatatt gaacaaaaat atatagatac tttttggaaa aatcttacta ataagtagag
      361 gagaaagaaa atgactataa aagtagattt acgaaaaaaa ccatataatt tagatgaaga
      421 tgctatttct tggattgaag agacgattga ttctatgact ttggaagaaa aaataggtca
      481 attgtttgtt aatatgggat ctagtcgtga taaaaattac ttgagtaata tggtcaatca
      541 gtaccatatt ggagctgtac gatataatcc aggaactgct gatgaggtat atgatcaaaa
      601 ctatatctta caaacagaaa gtaaaattcc tctattaatt gctgctaata cggaagcagg
      661 tggtaacgga gcttgtacag atggtactga agttggtgta gaagtaaaaa ttggtgcaac
      721 aaatgatgcg aaatatgctt atgaattagg gagagtttct gggattgaag catcggctat
      781 tggatgtaac tggagttttg ctcctattgt agatattaat agaaattgga gaaatcctat
      841 catttctagt cgatcttttg gacaaaaccc tgataaagtt ttagaactta gtttagctta
      901 tatgaaaggt attcaggaga gtggaatttt gcctgcagct aaacatttcc ctggtgacgg
      961 tattgacgaa cgagatcagc atctatcttt tagtattaat agtttaggta cggaagaatg
     1021 ggataattct tttggaaaag tatactcagg cttaatagaa gctggattac cttcaattat
     1081 ggcaggtcat atccatatgc cagcttattc aaaacatttt aatcctgatc tgaaagatga
     1141 agatttactt cctgcaactt tatcaaaaga attaatcact gacttactta gaggaaaact
     1201 cggatttaat ggattagtag ttactgatgc tagtcatatg ttaggaatga ctggggcaat
     1261 gaaacgatca gaattattgc ccacttctat tgcagctgga tgtgatctat ttctattttt
     1321 taatgatcca gatgaagact ttaattatat gatggaaggc tatcgtaaag gaattatcac
     1381 tgatgatcgg ttattagatg ccttaaaaag aattttaggg ttaaaagcta aattgggatt
     1441 acacaaaaaa gcaaaagaac aaattttatt acctaagcaa gaagctctgg aaaaaattgg
     1501 tttaattgaa aataaatcaa tttttaaaga ggtggctgac caagcaatta cattagttaa
     1561 agataaacaa gatatttggc ctattgtacc agaaaaatat aaaaggattt tactagtaga
     1621 tgtacatggg gtaaaaggtg ggtttggtga agtagttgct ggaaatagac ctcaagccat
     1681 tgatttattg aaacagattt tgtcagcaaa aggatttgaa gttgaaaaat acatttcacc
     1741 tgaagaagaa ttgaaaggat tatcgcaaga agaaaaaatt aaaaatatag ctggaatata
     1801 tgctgctaag agaccaatta aaaatttaac taaaaaatat gacttgatca ttaatatagc
     1861 taatgttaat ccaggcacag ttcaacgaat tgtctggcca gcaagtaaag gaactccgga
     1921 tataccgttt tacattcatg aaattccaac aatttttgta tctgtacagt gtccgtttca
     1981 tttggctgat gtacctcaag taaagactta tattaatact tatgatggaa aacaagaaac
     2041 tttagaaata ttggtggaca aactaatggg tctttctgaa tttaagggag taagtccaat
     2101 agatgcttat tgtggtttaa aggatacatt tatttaatat ttagatagga gtggtttata
     2161 tgttgtatcc cattatgaca gagacgaggc aagtaattga cttaagcggt ctctggagat
     2221 ttaaattaga tacaaatgat gacttacagg aagaacttgc tttagaacca ataaatgaaa
     2281 aagagagtta tccgatttca gttccctctt cttataatga tttatttgaa acagagaaca
     2341 ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gactcttcct aaaagattat
     2401 tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa gtttttttga
     2461 atggaaagtt tattactcaa cacaaaggtg ggtttacacc atttgagtct gaaattaatc
     2521 ggtttgtaaa agacgggaat aatcgaatta cagttgctgt aaacaatatt attgacgata
     2581 gtactttacc tgtggggatt atgaaggaaa cagtacagga aaatggggat ataaaaaagg
     2641 a
//
LOCUS       DAZKMZ010000173         2636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000173 DAZKMZ010000000
VERSION     DAZKMZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.179"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1060
                     /locus_tag="ACG8M5_002790"
     CDS             275..1060
                     /locus_tag="ACG8M5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0359974.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1073..1924
                     /locus_tag="ACG8M5_002791"
     CDS             1073..1924
                     /locus_tag="ACG8M5_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0359975.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2048..2500)
                     /locus_tag="ACG8M5_002792"
     CDS             complement(2048..2500)
                     /locus_tag="ACG8M5_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0359976.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT          825 a          435 c          561 g          815 t
ORIGIN      
        1 gggctgtgac aaaagtcttg tcacagcctt tttttccaaa taaatggttt ttcagaagta
       61 gcttcttcgg aaataagccg aaatttcttg aagctgacca cttctgtcac aacctctttc
      121 tttatagaaa taaaaggaca ctagcaattc atgaaaacga ttgctagagc gtatcatttg
      181 ctttagacta tatatcttgg tgtacactca ctttaaggcg gaaataaaag ttatccgtaa
      241 taacaggttt aggtatgaaa aaggagtggg tactatgtac aaggaattag aaaacaaagt
      301 cgcagtagta actggtggct caaaggggat tggaacagcg attgctcaac gattcgggaa
      361 agaaaaaatg aatgtagttg tcaactataa ctcggataaa gaaggagcag aaaaagcagt
      421 caaagcaatc gaagaggcag gcggaaaagc agtctctgta caagcagatg tcagttctga
      481 agaaggaata cagcgtttac ttgatacggc tttagaaaat tttgggacac tggacgtttg
      541 ggttaataat gctgggatgg aaaatcagca tccaacccat gaacttagcc ttgaagattg
      601 ggaaagagtg ttaaaagtca atttgactgg cgtttttatc ggaacgaaag cggctttaaa
      661 ttactttaca gagaataata aaaaaggaaa tatcatcaat atgtcttctg ttcatgaaca
      721 gatcccatgg ccaacgtttg ctcattacgc tgcttcaaaa ggcggggtaa aattatttac
      781 cgaaacggta gcaatggagt atgcaccgaa gggaatcaga atcaatagta taggccctgg
      841 tgctatccgt acacctatta atgcggcaaa atttgatgat cctgaacaaa aagaactctt
      901 ggaaagtatg atcccactag gacgcgtagg tgaaccaaaa gaaatagcgg cgacagctgc
      961 atggttggct tcagacgaat ccagttatgt tacaggtatt acactatttg cagatggtgg
     1021 gatgacttta tatccttctt tccaaggtgg aaaaggataa gggtgaataa aaatgggttt
     1081 attgattgca ttgatcccgg cagttggctg ggggatccag ccactgattt tgaaaaagat
     1141 tggtggcaga ccaacaaatg agatattagg aactggtatt ggagcggtgc ttgtcggctt
     1201 actcgttcaa ttattcgctt ctccagctgg tatttcagtg actacatttt tgattagttt
     1261 actttctggt gcattttggg ttattggaca aacaggacag tatacatctt tcaacttgat
     1321 tggtgtttct aaaacgatgc caatttcgac tgcattacag ttagtcggaa cctcgttgat
     1381 tggtgtcttc gcttttggag aatggtctgg aagtatgggg aaaatcattg gagcagcagc
     1441 aatcattctt cttgtatttg gttctgcttt aacggcaatc agtgatgatc ctggaaaaaa
     1501 aggcggtatc acaaaaggaa ttagtctatt agcatcaaca agtattggtt attgggtata
     1561 cagtgcattg cctaaactag ttgatgcaag cggactttcc attttctttc cgcaaatgat
     1621 cggtgtcttt ttaggggcag tgatttatgt gattttccgt aaaccaaccg cattttctga
     1681 agacaagagt tggaaagcaa cgatcgttgg agccatcttt agtgtgtcag cattggctta
     1741 tattttttca gcaaatgaaa atggggttgc tactgcatat atcattacac agctaaacgt
     1801 agttatctct acattaggcg gtatgtacat cctgcatgaa aataagacac caaaagaaaa
     1861 aaaattaacg attatcggat tattgctgat cgtagtagga agcatggtca cggtctttat
     1921 ttgaggataa agatggtaaa atttcattta ttaaaaaaat atttgtttga accaaacagt
     1981 aagtacttat aaaaaaatag cgctagaatt tccaacatct ctggaatttc tagcgctttt
     2041 taattgttta agatacaagg gaaatatctg tctggatcaa taatttttca ttgatccatt
     2101 gactaaagtt tacgacataa ttgaagttac caccgattag tatctccgca tgattgtctt
     2161 tataaagatc gtattgtaaa agacggtctt ttttatttgc gtaaacatcg ttatggtact
     2221 ggactaattg ttctcggaat tgattgattg ctgtaaaatc acgatattga aggttgaaac
     2281 gcatcgtgat tggtgcattt agcatgaatc gctgcatttc ttttgctgct tcctctgctt
     2341 gatctccgta taggaactca aaaagattac gttcagaaaa tttccattgc ccgccaacct
     2401 ttgttccttt tagttttcct tcatgaagat aatttctgat agaacgtgta gtaacaccta
     2461 attgttcggc taattcattt actgagtaaa aatctttcaa tgttcaccct cctatgtgaa
     2521 tggttctact gacttagttg atatttgctt aatcacgaaa tgtagaaaga acgattttaa
     2581 ttaaggtcat tgtaaaaatc aagtcagaaa attctaactt ttatttctct aaagaa
//
LOCUS       DAZKMZ010000174         2619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000174 DAZKMZ010000000
VERSION     DAZKMZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.180"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACG8M5_002793"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACG8M5_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACG8M5_002794"
     CDS             complement(349..672)
                     /locus_tag="ACG8M5_002794"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0359977.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACG8M5_002795"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACG8M5_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAZKMZ010000175         2611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000175 DAZKMZ010000000
VERSION     DAZKMZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.181"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..349)
                     /locus_tag="ACG8M5_002796"
     CDS             complement(<1..349)
                     /locus_tag="ACG8M5_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359978.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMY"
     gene            complement(356..904)
                     /locus_tag="ACG8M5_002797"
     CDS             complement(356..904)
                     /locus_tag="ACG8M5_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0359979.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1158..2477)
                     /locus_tag="ACG8M5_002798"
     CDS             complement(1158..2477)
                     /locus_tag="ACG8M5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0359980.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          813 a          498 c          421 g          879 t
ORIGIN      
        1 aatacattct tttccagtta catttaattt ttaaacaaaa ttctccccaa aaaggaactt
       61 tttctagtga aaacttggta atctctttgt aattaattct tgttaaagaa taattaaaca
      121 cttcattttt acttgttatt ttttcattga ctataactag ttcatctggt aaaaaaatta
      181 accaacaaac atcgacaaac agtaaatgat ataatccatt tccacctcca agcatattca
      241 atactggtga acatgcctta gaaacacgaa tatagttatg gtttaaatag atattttctt
      301 taaacattgc ttgtttaaaa gccatatgct cttgttctaa acttttcata aacttttact
      361 ctaatgcaca gctagcagca gctgctgcaa ttcctgctac aatatttacc ttagctaact
      421 ctttagctgc agcttttcct aattgttttt ctgcaatttt taataataaa tccgcagctt
      481 tttcaaaatt aaaagctttt aatgcttcaa taaaatctcc ctgcgtacct atagcccaag
      541 ccactcctcc tccaggaaga gaattaatgg ctacacatcc agcatatcgt ttccaatcaa
      601 ctttctccat tctaggaaca ataatatgtt cataaaagac tcttgctgct cttccttgtt
      661 cgttatcttg actgattcgt tcacgaatag caccaaaatc ctttatatgg tatacaccat
      721 gttcgtctaa aaaaccaact gtatcaaaat aaaaagttaa ttcatctact aatttattct
      781 ttatttctaa atcaactgta ttcacttttt ctgtggcata cacacttgtt ccagtactaa
      841 caaaagtact taaacctaat aagaaaacac ctaaaacacc aaatatttta attattcttt
      901 tcataatttc tcctccataa aaaataagtt actttttaaa tatcttgttt atgacctgaa
      961 taattcatag ctttaattct ttggcacatt ttattgaaat atgtatcact aatatcccta
     1021 tcaactgatt ttgactaaaa agttacttca aacattcact gttcaaaaat attgaggaat
     1081 aaattttggg tgtaaggcat tttgagcata cttctccctg gataacttcc cagtaaaaaa
     1141 atcgttagat tgttggatta gatccattga ctggctcgcc tcaggcgagc aaagaccctg
     1201 tagaattcat tctgatacac atgataacta gataatttga gatagactcg tctacccgtt
     1261 tggactaatt tcccagcaac tttcagaaac ttcaatcgaa tagaatgaat ggtcattccc
     1321 ttttggactt cttcaaagcc aatcgttctt aagaagttga ctaagttgta agctatcaag
     1381 ctgagcatca ttcggacatg attctccaaa aagcgaggac tgtctgtttt gtcaaagtaa
     1441 aagccagctt tcgcttcttt aatgaagttc tccattgtgc cacgtttggc atagatagaa
     1501 aagatggtat caggagaaac attttctgat agattcgtca cgataaattc atgtcgaaag
     1561 agtagttcgc ccgcttcacg tgttgagcgt atacacacgc gacgactttg tgaccaggat
     1621 tgtgcttgat aagtggtcga gaagtactga acttctcgtt cttcccactt ttgattatcg
     1681 ccataaagta ctgatttctc agctatttga cctagtctac gattattctt cagtcgaata
     1741 acataatgac tcttttttga ttcacacgaa tcatacacac caggtgtagc gaacccgctg
     1801 tctccacgaa ccaagatgtc agtgtttggt agagagtgat tatagtgctc taataaaggt
     1861 gtgagaaact cctttacgcc ttttgatgta tattgatttc ctgaacgtag ttcagctttt
     1921 aagaagtcac cagtcaatcc gtcaaaagct accaatggat ggtaaccata ggtttggtag
     1981 tgtgtattat aattcgtttg ttcttggtga ccaaatgtat ctgaatgggt cgaatctaaa
     2041 tcaatgatta attccgtatc attacggatg aggcgtgctt tatcgataag ttcttggttc
     2101 aaagcttgaa gttcatggat attctcctca gatagtcgat ctaaaaatcg ggaaagtgaa
     2161 gattgagaag cgagttcttt cctatctaaa acagctttaa acacaggatc ttgtctcagg
     2221 agattagctg cagaatcagc tgagtaacca gcaattaatt gcataatgaa ctgctcaagt
     2281 atggataagt tgtcatgagt aaaatacgct cgttcgtctt caatgtgaat gtgttgctta
     2341 gccagttcag aaaaaccaaa gatgttcatc agctctttca ctaggacgag acccgaatca
     2401 ctcgataatt gaccacctgt atgagaaata gtgatatttg aattgaattt tacttggttt
     2461 ttgtgtaagc tagtcatgaa aagaactcct ttcttttggt tggtttagtc actttaacca
     2521 tagcagaaag gggttctttt ttcatcactt aacgggtgaa gatgaaaaag taattgtaag
     2581 ctgccgtgag gcagtttcac atggttttaa t
//
LOCUS       DAZKMZ010000176         2606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000176 DAZKMZ010000000
VERSION     DAZKMZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.182"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..136
                     /gene="glyA"
                     /locus_tag="ACG8M5_002799"
     CDS             <1..136
                     /gene="glyA"
                     /locus_tag="ACG8M5_002799"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0359981.1"
                     /translation="KEEDAVKVAELVVKALQAKDDNAQLDEVKTGVRELTEKFPLHKK
                     "
     gene            289..1236
                     /locus_tag="ACG8M5_002800"
     CDS             289..1236
                     /locus_tag="ACG8M5_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0359982.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            1370..1999
                     /gene="upp"
                     /locus_tag="ACG8M5_002801"
     CDS             1370..1999
                     /gene="upp"
                     /locus_tag="ACG8M5_002801"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0359983.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2138..2380)
                     /locus_tag="ACG8M5_002802"
     CDS             complement(2138..2380)
                     /locus_tag="ACG8M5_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359984.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            2501..2573
                     /locus_tag="ACG8M5_002803"
     tRNA            2501..2573
                     /locus_tag="ACG8M5_002803"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2534..2536,aa:Thr,seq:cgt)
BASE COUNT          859 a          455 c          544 g          748 t
ORIGIN      
        1 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
       61 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
      121 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
      181 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
      241 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
      301 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
      361 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
      421 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
      481 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
      541 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
      601 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
      661 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
      721 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
      781 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
      841 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
      901 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
      961 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
     1021 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
     1081 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
     1141 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
     1201 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
     1261 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
     1321 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
     1381 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
     1441 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
     1501 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
     1561 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
     1621 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
     1681 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
     1741 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
     1801 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
     1861 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
     1921 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
     1981 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
     2041 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
     2101 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
     2161 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
     2221 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
     2281 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
     2341 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
     2401 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
     2461 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
     2521 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
     2581 agaagacaga tatatcaagg attttt
//
LOCUS       DAZKMZ010000177         2511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000177 DAZKMZ010000000
VERSION     DAZKMZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.183"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..330
                     /locus_tag="ACG8M5_002804"
     CDS             <1..330
                     /locus_tag="ACG8M5_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0359985.1"
                     /translation="ESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILA
                     DEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVN
                     KMNLEVE"
     gene            330..1706
                     /locus_tag="ACG8M5_002805"
     CDS             330..1706
                     /locus_tag="ACG8M5_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0359986.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1836..2249
                     /locus_tag="ACG8M5_002806"
     CDS             1836..2249
                     /locus_tag="ACG8M5_002806"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0359987.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2274..2511)
                     /locus_tag="ACG8M5_002807"
                     /pseudo
     CDS             complement(2274..2511)
                     /locus_tag="ACG8M5_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          929 a          350 c          455 g          777 t
ORIGIN      
        1 gaaagtatct tgtttgaact aggtttagat aaaaaacaaa taaaaagaaa tgttatgaaa
       61 ttatctggtg gacagcaaca aagagtagct attgctagag cattggtatc agatgctcca
      121 ataatactag ctgatgagcc tactggtaat ctagacagtg ttactgctgg agaaataatt
      181 aatatattaa agacattagc taaagataga aataaatgtg taatagttgt aacacatagt
      241 aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa gttgaaaaaa
      301 gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct tatgtaacta
      361 gaaaaagttt aaaatcatta attatcatat tagttatttt atctatgtca actttaagtc
      421 tcattagttt atctattaaa gatgctacgg acagagcttc aaaagaaaca tttgctaata
      481 taacaaatag tttttctatg gagataaata gacaagtaaa tccaggaaca cctagaggtg
      541 ggggaaatgt aaaaggtgaa gatattaaaa agatatctca aacagatagt atagactcct
      601 atgtaaaaag aataaacagt gttgcagatt tagttgatta tgatattatt gaaactcaag
      661 agactcttgc gaatcaatca cctgaaagag caaagaattt taaaagaaca gttatgttaa
      721 ctggggttaa cgactcaaca aaagaaacaa aatttgtatc agaagcatat aaattagtag
      781 aaggaaagca tttagaaaat gaagataaaa ataaaatctt aatgcataaa gatttagctg
      841 aaaaaaataa ccttaaagtt ggagataaga taaaaataaa atctaattta tttgatgctg
      901 ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta ttcgatgggc
      961 ataatagcgg tgtagtaagt gcagcacaag aactatacga aaatacatta atcactgata
     1021 ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac caagatgcaa
     1081 cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt gggaaattag
     1141 atataaattg gagagaatat aatttgatta aaagttcatc aaattaccct gcattacaac
     1201 aatctatatc aggtatttac tcaatttcaa ataaattatt ttttggctca ttaatatttg
     1261 caggagttgt agtttcatta ttattattct tatggatgaa tgctagaaag aaagaaatag
     1321 cagtattact atcattaggt atatcaaaat tagaaatttt tggtcaattc ctaattgaga
     1381 tggtatttat atcaatcccc gcattcgttg gttcctattt cttagctcaa tatacagctg
     1441 acaagcttgg gaataatata ttgaataagg ttactggtga tatagctaaa caaatagcta
     1501 aacaatctgc atctagccaa ttaggtggtg gggcagaagc tgaaggattt aacaagacat
     1561 tatcaagttt agatatcaat gtattaccta aattcatgat ttatgtagtt atatttatga
     1621 gtttagtact tcttgtatca ttgattattt cttcattcaa tatattaaga aggaatccaa
     1681 aagaattatt aattgacaat aattaaaatt ttcggtgctt tttagcacca aaaatgagag
     1741 tgtaatataa accgtgtaag taaccatcaa tcctaggcgg gtggttgctt cacggttttt
     1801 atttttctaa aataagatca agaggtgatt acacgatggc tagaaagaaa agaaatcctg
     1861 atgctgagaa gttagctgaa tccattttga atacttatca acccgaatct gtagaggata
     1921 tgcaagatgt tttaaaagat gtgtttggtc ccttatttga aaaaatgctt caaggagaat
     1981 tgaataacca tttagggtat gacgcccatt ctaaagagcc aaagaaacat gacaatcgac
     2041 gaaacggtta tggcaataag acgcttaaaa cgagctttgg taaagtaaac attgatgttc
     2101 ccagagaccg ggaggcttct tttgaacctg agttgattcc taatagaaaa cgagatgtct
     2161 ccgacatcgc agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc
     2221 tttttatcta cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat
     2281 agccaggtgg ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg
     2341 actttttcca agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca
     2401 aatattcgtt caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg
     2461 cgaagatgtt ccacttcatc catcagatct ccccaaacat gtctcgtgat g
//
LOCUS       DAZKMZ010000178         2455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000178 DAZKMZ010000000
VERSION     DAZKMZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.184"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..385
                     /locus_tag="ACG8M5_002808"
     CDS             149..385
                     /locus_tag="ACG8M5_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0359988.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            404..619
                     /locus_tag="ACG8M5_002809"
     CDS             404..619
                     /locus_tag="ACG8M5_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0359989.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1322..2074)
                     /locus_tag="ACG8M5_002810"
     CDS             complement(1322..2074)
                     /locus_tag="ACG8M5_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0359990.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2089..>2455)
                     /locus_tag="ACG8M5_002811"
     CDS             complement(2089..>2455)
                     /locus_tag="ACG8M5_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0359991.1"
                     /translation="ICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDREL
                     SLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNM
                     FVNYQNDPYQFTTQVILLF"
BASE COUNT          800 a          391 c          408 g          856 t
ORIGIN      
        1 ttgaacacta actttttcat tcctgaatca aatttatttt ggaaatttta ctattcaaga
       61 ataaacttga taagtttcag gaagattttt actgttaatc aaatacgtat gttacagtag
      121 caccgtaaaa aatattattt aaagaagcgt gtttaaacat gcaaaatgta aaggaactaa
      181 gtattgtgga aatgcacaaa ctgttggtgg aggcaataat gcttggggaa aattggggca
      241 agttgtaggt ggacttacca caggggcagt aggtggtgca ggtttaggaa cagcaatttg
      301 cggaccagct tgcggtgttg ttggagggtt atatggagct gtcgctggag gtgctgcagc
      361 tggctgggat gctcgaaaaa aataaaaaag gagaataagt agtatgcata ttaaaaacac
      421 aaaaacaaca tttatattat cttcagaaga gttgaaaaat attcaaggtg ggagtgccgt
      481 gggagtttta ggcactactt tttctggggc aacagcagga gtaaaacttt gttcagccgg
      541 aggaccttat gcaatagctg cttgtggtgt aggtggagct ttattaggtg ctggattcag
      601 catgtggacc ggtgcgtaaa caattaaaaa atatcaaacc tcaagattgg tttgtgtcga
      661 ttacgaacat aggtatgttc tttatctttt caaaagtttg gaatggacag aactttttac
      721 taaagcttct tgtgctagtt tttataatta tattaaattt ttatctttta ttaaagtcag
      781 ataaaaattc tttggaaatg taatggtaga gatattttta caaaaggact tgcagcgctg
      841 tagttggtgc tgcaggagga cctatttcgg ccgttacagg aggtattggt aatgtacttg
      901 tcggatgtgc attaaagcct gttcctgcaa gttaaattta taaaaacagg aaagtaatta
      961 tctcaaatcc tatcaagtac aatcatagga ttgcacttga caggacaaat tttttaacta
     1021 aaaaatgaaa aacactttct aaaaattagt agaatttgcc aaatctttcg catcttccac
     1081 catacattta attttaattc ccctttatgg tacttaataa aaacaagaaa agaactattc
     1141 atttggccct tttttggttc cagaccatag aaatattcct ccagtaacta atattgctgt
     1201 tatgcacgta actaataaca gctataaaaa ttggtacaac tttaatagga ttcagaaaaa
     1261 tttgcactag tgaaggaatc tattttatta gaaaaatgca atctccctaa agaaaaaacc
     1321 actaaacagt gctttctttc aacttctcct taattttttg tgctttccct aaagtaaagt
     1381 ttctggccaa ctctgaatca aaatatagtg aacgtttttt aaaatctatt tctttaacgt
     1441 taaagatatt tactaaacac gctcgactaa ttcttgttaa cataggatat ttcttttcaa
     1501 gctcactaat tcttccataa aattcgtact gcccatcttt agtacacaat gataatctat
     1561 gtggtaattt tgaagcttct acaaaataaa tgtcatttat atcaaaagta aatgtttgag
     1621 aacctatgga aaaaacaaat gcttgattcg taacctgttt tgttgcatct atcctttctt
     1681 gcgctaataa caataactca acaatctcgt ttcgatactc atctagtgtt tgatcttttg
     1741 ttacaaatcc taacgtttca actcttcttt ttattgttac gggtaacatc tcatcatgag
     1801 tagttgtaaa aatgattttt gcttgaacat catattttct gattacttct gctaattcta
     1861 taccgttaac ttcatgattt aaatcaatat ctaagaaata tattccttgc tttggtcgga
     1921 attgtttgag gtatttttta acttctaacg gactttgtgt tttcaatact atcttaaata
     1981 ctttatcgtg aaaaagaatg aaattatcaa taattcgttc taactgattt aattgtataa
     2041 tttgatcttc acataatatt attggataac tcataaatcc tccttccctc aaaacaacaa
     2101 aataacttga gtagtaaatt gatacggatc gttttgatag ttcacaaaca tattgggatt
     2161 ctttttattg atttcctcta tactagataa acctaaccct tgatgtccct tttttgttgt
     2221 tattcctttt ttcattagag tatctataga gagtagtatc tctctacaac tattcgatat
     2281 cgaaatttct atctgtttat catctcggta aatagctaga gacagttctc tatcttgact
     2341 ctctatagat gcttcaattg cattatcgag aataattcca agcaccctaa tacagtcaaa
     2401 tattggaaca ggaacttcaa taatagtact tggacattcg aatttacaac aaatc
//
LOCUS       DAZKMZ010000179         2441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000179 DAZKMZ010000000
VERSION     DAZKMZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.185"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8M5_002812"
     CDS             complement(573..1130)
                     /locus_tag="ACG8M5_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359992.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8M5_002813"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8M5_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359993.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8M5_002814"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8M5_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0359994.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          714 a          526 c          322 g          879 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc t
//
LOCUS       DAZKMZ010000180         2419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000180 DAZKMZ010000000
VERSION     DAZKMZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.186"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG8M5_002815"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG8M5_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG8M5_002816"
     CDS             1321..1779
                     /locus_tag="ACG8M5_002816"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0359995.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG8M5_002817"
     CDS             1938..2408
                     /locus_tag="ACG8M5_002817"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0359996.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          456 g          699 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DAZKMZ010000181         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000181 DAZKMZ010000000
VERSION     DAZKMZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.187"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8M5_002818"
     CDS             207..2069
                     /locus_tag="ACG8M5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0359997.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8M5_002819"
     CDS             2069..>2398
                     /locus_tag="ACG8M5_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0359998.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKMZ010000182         2396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000182 DAZKMZ010000000
VERSION     DAZKMZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.188"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(263..562)
                     /locus_tag="ACG8M5_002820"
     CDS             complement(263..562)
                     /locus_tag="ACG8M5_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0359999.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            913..1545
                     /locus_tag="ACG8M5_002821"
     CDS             913..1545
                     /locus_tag="ACG8M5_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0360000.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1668..2306
                     /locus_tag="ACG8M5_002822"
     CDS             1668..2306
                     /locus_tag="ACG8M5_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0360001.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          809 a          377 c          339 g          871 t
ORIGIN      
        1 ttttcaatct atatttccaa tttttaactt acaaaaatgc aacattaata aataacgata
       61 gtttcataaa atacactttt aattaacatt attagtttat actagctgtt tttgtgttaa
      121 ttaaaggata aaatacaata taaagaaaca tctgtttctt tatttagaat aaaaaaatac
      181 agactctgtt caaataaatg aacctagcct gcattatgag attgactata ctttttaatt
      241 cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc gaagccattc
      301 ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa aacggtgata
      361 ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc aaattattta
      421 tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc aaaagtatac
      481 gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt accatgtttt
      541 ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct tcccactaca
      601 ttctataaac aaagtatacg ctacatctag atttttttaa atcattactg gattgttaaa
      661 acctttcaat acactgttat ggcaacgatt tttattttaa aaacattcta aattaaatca
      721 gtaaaaattt ttattctaag atttttatta gaattacgcc taatttgtgt ctctcagggc
      781 aattctaaga acgtttcaag agatagcgac actatcgtta tcgaaaaagg cgaaaaccta
      841 tctcattttc tgctaatgta agttggttta gcttttgtta atatgacttt atgaaaatgg
      901 aggtaattaa atatgatttt tcctaaagtt aatgaagcaa gcttgaataa aggatccttt
      961 tttcatatct atgaaaacga agattttgaa ctgtataata tcctttcaca aaaagaggtc
     1021 atcgaaaacc aaataatctg ccatccaatt atttacaaat ctattttcaa gtatgcattt
     1081 tttgcttcca atgattttca aagaatggag attaccgact gtatattcga aaactgtgat
     1141 ttttcaggct gtatattgac aaaaagcatg ttgcatcgcg tgaaatttgt caattgtaaa
     1201 ttaacaggta ctaaattgat ggaaagttat attgggaaca cgttattcga gaattgtaaa
     1261 atggactatg taaatttatc tggttcaaac ataaaagaaa gtacctttga gcactctgtc
     1321 ttatcaagcg cagattttgt agactgttcc ctcactaaaa taatgttttc tactaatgat
     1381 ttagaaaatg ctagcttttt tgatactgat ttaaaaaata tcgatttaag tagcaactcc
     1441 tttgaaatga ttgaagtacg ctcggaacat ctaaaaaatt gtacgcttaa tcagttgcaa
     1501 gcacttggtt ttgctagaaa atttttacaa ataaaagtag tataatcaaa atgaatatat
     1561 aagataaatt tttagcaata caaatatcca taaaaaaaca ttgcctattt taactcttca
     1621 tgtatactga ctctatcttc aaaaactgaa tagtaggagt tattatcatg aataacattt
     1681 taggaaaaaa aatagaactt aaaaaaggga tattgggcac tattgtcgtt gtactcggtt
     1741 tgttagggtt gctatttgcg ccatccatta ttagcatttt ttctttaact atcactggat
     1801 ttatagcatt tacacttgtt tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg
     1861 tcgctcctgt caaaaatata gccaaatatt tttttatcaa tgtagtaatc agtgccgcag
     1921 tctcggtatt tttgcaacaa attttgaaat ggggtttaac aggcaatcca ataaatgagg
     1981 ctttcagccc gttattattt attattttgc ctatcatgat acttggagaa gaattgttct
     2041 cagtgtattt tctcgctatt ttttctagta agttcagcat tcctgtcgct agtttcttgt
     2101 cagcaatcat ctttggattt atccattaca gtacttatga taatggaaat attttacaca
     2161 ctgtagcaca catccttttg atacaagggg tggcaagatt gttattcaac caagctgcca
     2221 ttaaaagcaa ctcgatcatt acaagttggg cagttcatgt gatatttgat ttcacagcta
     2281 tattgctagt cgttttattc tcctaaattt ttatagaaat aatacatgct ctattgctaa
     2341 gaaagcatac tttatttcct ttaaacaaaa agacagatgt atcaatggat taaaaa
//
LOCUS       DAZKMZ010000183         2386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000183 DAZKMZ010000000
VERSION     DAZKMZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.189"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..996)
                     /locus_tag="ACG8M5_002823"
     CDS             complement(1..996)
                     /locus_tag="ACG8M5_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0360002.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1014..1568)
                     /locus_tag="ACG8M5_002824"
     CDS             complement(1014..1568)
                     /locus_tag="ACG8M5_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0360003.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(1556..2047)
                     /locus_tag="ACG8M5_002825"
     CDS             complement(1556..2047)
                     /locus_tag="ACG8M5_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0360004.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            complement(2040..>2386)
                     /locus_tag="ACG8M5_002826"
     CDS             complement(2040..>2386)
                     /locus_tag="ACG8M5_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFQ0360005.1"
                     /translation="EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITD
                     QGLAETGGNGVIFLFAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFL
                     KLIKSKGERLHE"
BASE COUNT          681 a          522 c          394 g          789 t
ORIGIN      
        1 tcagctatat aatccaaaac ttgctaccca agcaatgata actcgtggga tgccgttaag
       61 aaatcttgaa taaagaactg atggtacacc tacttctact gtctcagctt ttgcttcttc
      121 caaacctaga ccgactggaa taaaatcaca tgcattttga gtattgatag caaatagtgc
      181 cggtaatgct aattggggag gaatatttcc tttcccaatc tctacaccga tcaatgttcc
      241 aatcacttgg ctaataactg cccctggacc aagcaaagga gataaaaatg gcagtgaaca
      301 aataaagcca atgattgtta atccaactgc atttcctgct aaaggtgcca taatgttagc
      361 aaatacatta ccgatcccag aaccttgtat cacaccaatc aaaagggaaa cgaaagccat
      421 aaaagggatc actgtccgga tcatcgtatc tactgcttct cttgctgatt gattgaatgt
      481 agcaactact tttcccgcgc ccattccaat ttttgctatc cagttttgct gctctgcttt
      541 ttgctcggtt atctttttag aagtatcata tttgtacttt ttatctggag atgcagtatt
      601 tactgtttct ttgttatcat cagtttccat tagtggctgt atttgttcta accctactgc
      661 tgaaacataa atatcttcag tgatatattg tgctaatggg ccactttttc ctgtggaaac
      721 gatattgatg gttggtatcc cttttttagg ataaattcca catctaagag tccctccaca
      781 gtcaacgatt gcaagcgcga tttcactatc aggtattgat gttttgaacc cattgataga
      841 ctccatacct gtcagttctt ctattttatc tacgatcatt ggcctctcac ctccacctgt
      901 gacatagatg aacttgtgtt tatcttttgt cggcctaata atcaaaggac ctccataccc
      961 gccgtttcct cttacgatct taatatcacc atacatttaa aactctcctt tctctaatca
     1021 actgaaagct ctactttttt gctaagaacg attccttgct gtctgcatac aaatccagta
     1081 gtaaaatcag ttatccaacc accaataaag ttcatcagca gtcccacaag caagtatcgg
     1141 atagccagct ccatcgaatt tagccctaag gtttcgatcc cttgagcaat ccccatccat
     1201 acaaacaatt ctccaggatt aatatggggg aatacaccat tgctcgtatg acaaaattga
     1261 gcttgagcag cataatagct tggtttgtag tattctggaa ggaaacgagc cattgtaaat
     1321 gacataggat ttcccaacat aaaagcagat aagaacggta acacaaggta acgagtaagc
     1381 ggatttttcg ctgataattt ggctactttc gttactcggt cttcacctaa aaaagcaatc
     1441 agcgtattca ttgcaaccat cagcatcaag acaactggta cgattcctgt catccagctt
     1501 atgaatgttt ctgcacctgt ttggaacagc ctcataaatc cctcagcaaa ttttgttatg
     1561 atatccatcg tttgtcactc cttttttctt tgatcgtact tttccatatc cgaaaattat
     1621 tgagcatagc aataaaaggc aatgaaggtt gttcactttt ttcatttcct gcctctattc
     1681 gtaagtatac ttctctggca ttttctaaag ctgcttgtgt caaacgatct tccgctcgaa
     1741 ctgagggatg gtaacgatcg atgtaatgga tatcttcacc cgcatagaca ggtaatgaat
     1801 gaaattttgc caaagatgta accccttgca tttttctgac ttctaaaact tgatcatgat
     1861 cgtctatagc tagcataaca atcgtgcctg aacggatttt cccaggtcgt ttaccaatcg
     1921 ctacttttcc ttttcttcgc agttcgctat atgtttgatt gaaatgcttt agttgcttcc
     1981 aactgataac tgcttgcagc acataagctg aagctgcgaa aactatgaat agatacatat
     2041 cattcatgaa gtctctctcc tttacttttg attagtttta aaaatgattc ttgtgtcggt
     2101 gctaattcaa taaactcatc tattcccatt tttatgagtt caaataggat atcgtatagc
     2161 tcaaataatt cattctcaac ttcagccgtc atttcttcag gtatggcaaa taaaaaaata
     2221 actccatttc ctcctgtttc tgctaaacct tgatcagtta ttagccctac tgataaaaca
     2281 atgtgttgag atagctggtt ggttgtgtga ggaaaagcaa attctttgtt aacaaatgtg
     2341 ctttgctgat attctctttt caagatcctt ttttcaaatt cttcat
//
LOCUS       DAZKMZ010000184         2335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000184 DAZKMZ010000000
VERSION     DAZKMZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.190"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8M5_002827"
     CDS             complement(51..1598)
                     /locus_tag="ACG8M5_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0360006.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8M5_002828"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8M5_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0360007.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG8M5_002829"
     CDS             complement(2043..2237)
                     /locus_tag="ACG8M5_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360008.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          653 a          483 c          420 g          779 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga agacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttaaaaatgt tttgc
//
LOCUS       DAZKMZ010000185         2295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000185 DAZKMZ010000000
VERSION     DAZKMZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.191"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..351
                     /locus_tag="ACG8M5_002830"
     CDS             <1..351
                     /locus_tag="ACG8M5_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0360009.1"
                     /translation="EFTNFFVSEFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFII
                     KTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRK
                     EHYYSEKNTKGEIL"
     gene            348..1067
                     /locus_tag="ACG8M5_002831"
     CDS             348..1067
                     /locus_tag="ACG8M5_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0360010.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1082..2167
                     /locus_tag="ACG8M5_002832"
     CDS             1082..2167
                     /locus_tag="ACG8M5_002832"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0360011.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          835 a          247 c          454 g          759 t
ORIGIN      
        1 gagtttacca atttttttgt cagtgagttt tataaatcgt ttggtaatct tcattatcag
       61 tattttcatc atatttggtt atttatagaa gttggaatat taggagtttc gatattccta
      121 ttttttataa taaagacatt tttcaatgca aaaagatctt ttatgaagga taaagatgca
      181 agtcttgtta aagttattgc aattatgtcg gtattctttt ttgtgtataa cacaacactt
      241 aggggtgaaa caacggggcc actgatttat atgattttag ccatgcctga agttgtgaaa
      301 aggaaagagc attattatag tgaaaaaaat acaaaaggag agatattatg attcctaaga
      361 agatacatta ctgttggttc gggggaaatc cacttccgaa aaaagataga aagtgcatag
      421 aaagctggaa aaagtattgc cctgattatg agattattga atggaatgaa tcaaattatg
      481 atttaaataa gaaccgttat atggccgatg catataaaga acgtaaatgg ggttttgttc
      541 cagattatgc aagatttgat attatttata atcatggtgg attttattta gatacagatg
      601 ttgagctaat taagtcgtta gatatacttc gtgaaaatca tgcctatatg ggatttgaag
      661 gggaaatctg ggttaatggc ggtattggtt ttggtgcaga agaaggacat tctgttatta
      721 aaggtttaag ggatatttac gattcattaa gtttttatca aaatgatggt acattgaact
      781 tgaaaccgag tccgtattat attacagatt atcttgaatc tattggattg gttcgcgata
      841 acagcgaaca aataataaat ggcctgcata tctatcctac ggattatttt gctgccaagg
      901 attatgatac tggaaaaatt tataaaacta gtaatacaat aagcattcat caatataatg
      961 catcttggaa aacacctaaa caaaaatttg tttatagatt aaaatcaaca attggacaaa
     1021 aaaaatttaa caggttactt gcactgaaaa aatttttaca cttataaagg agaaaagtac
     1081 tttgaagaaa atttgtttca ttagtggtgt aatttctcgt tctgggggaa ctgaaagagt
     1141 agggagtatt attgccaata gcttgtctga tttaggttat gatgtcacta ttttaagctt
     1201 ttggaatagt ggagagcctt attttgggtt agataagacg attaaggttg attatatgtt
     1261 aaatcctaaa acggagggaa aattatatag aacttattta tatccggttt ggaaattgca
     1321 caagtatata tgcaaaaatg aatttgatat agtaattgat attgatactt tgttgtcttt
     1381 gtatactaca agggcaatta aaggaacaaa gtgcaaattg atttcttggg aacattttaa
     1441 ttactggaca atggaaatgc tgaaagaaaa gaagagattc aaggctaaaa aattgataaa
     1501 aaaatatgca aagaggcttg tggttctcac agaagaagat cgtcaaaaac atattgatga
     1561 atatagttta tcagaagatt ttgttataac tatgccaaat ccgtgtatat ctaatatcga
     1621 gtacgaatat cattatgata aaaagagatt tcttgcagta gggcgattag tggaagagaa
     1681 aaattttgct gacatgataa gtgcttggaa aattgtagaa aaaaaagtag atgattggga
     1741 tcttatcatt gtaggaaaag gtgaattaga aaaagatctt cgccagcaga taaaagattt
     1801 aaatctgcaa agagtaacta tagcagggca ttcagaccat gtggaggaat attataaatc
     1861 tgcatcttgt tatgtgctta gttctgttta tgaaggattt cctatggtta tattagaagc
     1921 acagtcttat ggattaccag ttatttctta tgattgtaaa actggtccga gagatttggt
     1981 acatcataat tttaatggga tgttggttga agataagaat atagaccaat tagctaagag
     2041 catgataatg tttactaaga atactgattt agcaatgaga atgtcactga acgcatataa
     2101 taatgtacaa aaatttaatt tgaaagaaat tacaaaacaa tgggttgctt tgattgaaaa
     2161 tgtatagttt tttgtggttg tccaaacatg aaaattagcg taattaatca aagagctaga
     2221 agaaacctat tacatttcaa gattttagaa attatatagt attaggtaac aataaaaaat
     2281 agaaagtttg gagcc
//
LOCUS       DAZKMZ010000186         2246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000186 DAZKMZ010000000
VERSION     DAZKMZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.193"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8M5_002833"
     CDS             <1..1125
                     /locus_tag="ACG8M5_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0360012.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8M5_002834"
     CDS             1474..2106
                     /locus_tag="ACG8M5_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0360013.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          836 a          307 c          451 g          652 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaatt
//
LOCUS       DAZKMZ010000187         2244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000187 DAZKMZ010000000
VERSION     DAZKMZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.194"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..200
                     /locus_tag="ACG8M5_002835"
     CDS             <1..200
                     /locus_tag="ACG8M5_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0360014.1"
                     /translation="VTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGI
                     TRQGATNLKHRLYKKIQKMLV"
     gene            294..485
                     /locus_tag="ACG8M5_002836"
     CDS             294..485
                     /locus_tag="ACG8M5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0360015.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            999..1331
                     /locus_tag="ACG8M5_002837"
     CDS             999..1331
                     /locus_tag="ACG8M5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0360016.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1241..1435
                     /locus_tag="ACG8M5_002838"
     CDS             1241..1435
                     /locus_tag="ACG8M5_002838"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0360017.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            1474..1620
                     /locus_tag="ACG8M5_002839"
     CDS             1474..1620
                     /locus_tag="ACG8M5_002839"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360018.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2058..>2244
                     /locus_tag="ACG8M5_002840"
     CDS             2058..>2244
                     /locus_tag="ACG8M5_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0360019.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT          807 a          376 c          383 g          678 t
ORIGIN      
        1 atgtgactct ccttgttgaa gatataaaat tagcaaaaat ccttcctttg ttgaaagaac
       61 ctgaacaaat ttttcttatc gaaaaattta tctttgataa aactgataaa gaaattggaa
      121 aggttctggg gattacaaga cagggtgcga caaatttaaa acatcgactt tacaaaaaaa
      181 ttcaaaaaat gctggtttaa tatctgcata ataactaatt ttgtatgctt ctttgctcgt
      241 tctcctcctt cccaaagaaa tagattataa gtccatggga agaaggtaga tttatgaatt
      301 ctttaaaaga agatttcatt ttagcaaagg ctggaaatga agaagctgtt gaggcaattc
      361 tcaaaagatt tagctctttg attcataagc aatcctggag aactggaaaa tatgatcaag
      421 attgttatca agaatgtatg cttgcaatat atctagctat ctcgaaattc gaaattaaag
      481 aataactcaa ctaagggagt ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa
      541 taaatttgaa actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg
      601 attcatagaa tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct
      661 taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg
      721 caggaggtcg aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt
      781 gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc
      841 aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta
      901 tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc
      961 ttgactatca gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta
     1021 aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg
     1081 ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat
     1141 acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca
     1201 ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga
     1261 tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa
     1321 acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg
     1381 caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga
     1441 taatttgaac agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt
     1501 aatgacggtc gcctctattt gtccacctat attgacttag caactcgtat tctacgttac
     1561 tatatggtag attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa
     1621 ggagaattgc ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat
     1681 caagagctgt tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt
     1741 gacaatgcag taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac
     1801 aaatataaaa gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac
     1861 ccaaacaaaa gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa
     1921 ataagggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     1981 ttctaataaa cgcaaacaga caccttaaaa tcctttagaa taaagtcgac gaaaaccatt
     2041 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     2101 acctaaatct tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat
     2161 gttttgtttt tgatggcaca ttgacctatc aaccggagga atgctttcat cgccactatc
     2221 aaaatgaaca aacgatcatt aaat
//
LOCUS       DAZKMZ010000188         2229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000188 DAZKMZ010000000
VERSION     DAZKMZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.195"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1508
                     /locus_tag="ACG8M5_002841"
     CDS             <1..1508
                     /locus_tag="ACG8M5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0360020.1"
                     /translation="KRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNNLFTSIF
                     QMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLGLLCIPF
                     LRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVSMLITFT
                     FQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELEKETIDD
                     IIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIFAIIYES
                     CIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVGSDNLMS
                     ISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILVNWFGLY
                     GVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYLSYFACS
                     YVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            1686..1907
                     /locus_tag="ACG8M5_002842"
     CDS             1686..1907
                     /locus_tag="ACG8M5_002842"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0360021.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          746 a          235 c          426 g          822 t
ORIGIN      
        1 ctaaaaggaa tataattact ggaacaattt ttaagttata tcagattatc attccctttt
       61 ttatgcgtac acttatgatt tatcagatgg gaatggagta tgctggattg aacaacctgt
      121 tcacatctat atttcaaatg ttaaatttag cggaattggg aataggtgct gcactaactg
      181 taagtatgta taaacctata gcagaagaca atagaaaaga gatttgtgct ttactgaacg
      241 catataaaaa gtacttttca attattggat ggatagtgtt tacattagga ttgttatgca
      301 ttcctttttt gagattttta gttaaaggtt cgattcctgg aaatttaaat atgacagttt
      361 tgtatttaat gtatttgata aatactgttt tgtcgtattg gttattttca tatagaaaga
      421 gtctaatata tgcacatcaa agaaatgatg taattaacgt gtctatgcta attactttta
      481 cattccaatt tgtaatgcag gcatatgttc tgattttctt aaaaaattac tatttgtatt
      541 tattggcttc tattttggga caaatagttc ttaacttaat gtgtgctgtt gtttctcaaa
      601 agatgtatcc tgattatact cctgttggtg agttagagaa agaaactatt gacgatatta
      661 taaaaaaagt aaaaggatta gttacaaaca aggtaggcgg tacaatatta cgatcttcag
      721 attcaattat tatttcatct ttcttaggtc tttcagtact tgcgatttat cagaattatt
      781 actttattat ttctgccata atgagtatat ttgcaataat ttatgaatca tgtatagcag
      841 gtataggtaa cagtcttatt gtagaaagtc aagaaaaaaa ttatatagat ttttgtgata
      901 ttacaacaat tataggttgg tttctggcaa ttagttgtgc atgctttatt acattatttc
      961 agccgttcat gattatctgg gtgggaagtg ataatcttat gagtatttca tttgttatat
     1021 ttatgatagt tcattttttc ttatatgaaa ttgatcagtt aattgggtta tacaaagatg
     1081 ctgctggaat atggtacgca gacaggttta gaccattaat tacagcaatg ataaacattg
     1141 tattaaatat catcctcgtt aattggttcg gattatatgg agttttggcg gcaaccattg
     1201 tttcagtatt gttggttgat ttaccctggt tattatataa tgttttccaa aatatattta
     1261 aatttgaatc ggcaggtaaa tatgtttgga agctcataag tttgtgtgta atagcttttg
     1321 ttgcttctta tttaagttat tttgcttgtt cgtatgttaa tatcggaaat ttcaaagtga
     1381 taagtttagt gataaattta gttatatcgg ttgtcatatc gattttagtt attcttctgt
     1441 ttaatatccg taataagaag tttaagtata ttttgaaagt tcttacgtcg gtattggtaa
     1501 aaagatgaga agagacatca gaatgatata taaagtaact acaattttta ggctgccgaa
     1561 taggcgaacg atgtatagtt tttgttccaa cgaagactct tgttgaaatt ggagatgata
     1621 tgaaggagtt acaattttga ctcatgttta tgattgatcg gttttaaaag tagtatggag
     1681 aagctttggg atcatcggag aggatcaaaa taggtaataa tgtttttata ggaatgaagt
     1741 ctagaatact aaagggtgta catataggaa ataatgtagt tataggtgca aatagtttag
     1801 taaacaagga cgtatcagac gattgtgttg ttgcaggaaa tctagcaaaa gtaattatgt
     1861 ccctagatga atattataaa aagataaaca atatgaagaa tccgtaaaac ttgtgaaact
     1921 ataccgagaa taatatggga aaaatattga tgaaaaagca ttatatgagt ttttagggct
     1981 gtttacagat ggagatgaca atttgtcgaa gagttgggag caatgcaaaa tttagtaggg
     2041 gattataaac agactgacaa ggtatttaaa gaacatgcac aagtatttca taatattgaa
     2101 gaatttttga atactgtgag ataggagttg ctacattgag aaaagtatgt atttttccaa
     2161 tgtaaaagat tacgagtttg aaaacgacac cataatatta ttttttaaga agatattata
     2221 gagcttagg
//
LOCUS       DAZKMZ010000189         2134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000189 DAZKMZ010000000
VERSION     DAZKMZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.197"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..236)
                     /locus_tag="ACG8M5_002843"
     CDS             complement(66..236)
                     /locus_tag="ACG8M5_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360022.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(233..583)
                     /locus_tag="ACG8M5_002844"
     CDS             complement(233..583)
                     /locus_tag="ACG8M5_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360023.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(576..1901)
                     /locus_tag="ACG8M5_002845"
     CDS             complement(576..1901)
                     /locus_tag="ACG8M5_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0360024.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT          622 a          436 c          326 g          750 t
ORIGIN      
        1 ataggtaatt ttatcagaat tgatacaaaa tgtaaaacat aaaagaaata taattttatt
       61 gcactttaat agccaatata gtattcgtat ttatggtatc ctgctgcagc ttctacatag
      121 aaagagaagc gttcaacacc aacctcattt atcaaacgct ggccataacc ccaaatctct
      181 tctgatagct gatcaaaggt tatatacttt agctcatatt gctggataat gttcattcta
      241 atcgacccca tttattatcc tggtaaaatt cgatatgacg tatttcgtcg tagtgaactt
      301 tgttttttcc aacgtagatc cccagctcat cactacctaa gatcttgcta acaatatctt
      361 ctggatagta tccttcagcg tttcgttctt ctttttgaat acatactgat ctatttttta
      421 tgactgcagc gttcaatact tccgcaacct cttcagtcgt catttgttct tttgccaaat
      481 tttgattggc tcgttgttca ttgtcggcat caattttttc agtatgatca gataaataaa
      541 aacctgccca ctttaaacgt ccacgatcat tataatcaat catttttaag ccctcctgcg
      601 ccgcctgcat ggccaccaac caatgtactt ctagtaatac tacgagcgcc ttctaacata
      661 gaagaagcat gtacaatcga cgtaaaacca tatttttgcc gaatcttgtc aataatttta
      721 tctaaatttt cttcgtcaat ttgcttttgt ggatcactaa ataaatccag ctgaagcgaa
      781 tcagtgtaca caagcttgct ataggttatt ccaacgtgcc ttacttcttg tccttcataa
      841 tacttatcaa acaagaaaag acagtaattc tgcaattcct ttgtattatc tgttataggg
      901 atcttcatct ggtgtgaaaa acctggtctc gtttctaaga tcgacgtgcc aatgtataaa
      961 tggacacaac ctgttttaca gttatgttgc ctgatacgaa tagctacttg ttccgccatt
     1021 tcttttacca ccagttcgat ttcatttctc ctggtataat ctcgtggtaa cacttgtgaa
     1081 ttaccatatg atttttcctt tgtcggttct ggtgggatcg ctatatctgt tcgatcgatc
     1141 ccgttcgcat gaaaatacag ctgtaagcca atcacaccta aatggctctt aatagtgtat
     1201 ggattccaat tagctaagtc tttaatactc atgataccaa tttggttgag tctttttttc
     1261 attcgagatc caatacccca aaggtctgtc atttcaggaa tattccatac tttatcagga
     1321 acattttgat acgtccattc agcaaggaat ccatcattat gttttgcttc attgtctaaa
     1381 gcaagtttag ctaataaggg attatctccg acacctactg tagcaagcaa tcctagctct
     1441 tctttaatat tctcctggat catttgagcc agcttctttc tccgttgact tcttgttcct
     1501 tcagtcgtaa aaagattcat tgatctggtc acttttaaga tcgattcatc gatcgagtaa
     1561 atcaataaat cttcgtctgc tacatacctt ctgaaaatat tgtttacctg catatttcgc
     1621 ttaatataga gattcatgcg tggtggaacg acatgtaacg ttttaggaaa aggttgcggt
     1681 agatctcgtg gtcgactgac attagaaata ccgtacttct tctttgcagc tggtgaagaa
     1741 gctaaaatca accctgatcc tgtattatcc gctcgactca tgacgactaa ttcagtcgtt
     1801 aatggatcta aattcctttc tatacattcg acacttgcgt aaaagctttt catatcgatc
     1861 aaaaagtaat catttactgg ctcttttgaa taatccatca taaataacac tccattcttc
     1921 cacattatac aaacatacgt tcgtttttgt aaagaagaat cgattaaatg tacaaaaaaa
     1981 gaagcatagg taagttattt ccctacttct tctgttgatt tgtaaaatca acacattgta
     2041 tcactttatt aaatggatat ttactattag tcttgtgaaa atttgaaaat aagtgtcgta
     2101 ttatccagtt ttgcttagtt atattagaaa aaaa
//
LOCUS       DAZKMZ010000190         2116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000190 DAZKMZ010000000
VERSION     DAZKMZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.198"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..1198
                     /locus_tag="ACG8M5_002846"
     CDS             26..1198
                     /locus_tag="ACG8M5_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0360025.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(1360..1854)
                     /locus_tag="ACG8M5_002847"
     CDS             complement(1360..1854)
                     /locus_tag="ACG8M5_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360026.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1851..2045)
                     /locus_tag="ACG8M5_002848"
     CDS             complement(1851..2045)
                     /locus_tag="ACG8M5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360027.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT          628 a          386 c          430 g          672 t
ORIGIN      
        1 acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac tggacatctt
       61 catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg tagaagattc
      121 actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag catcgaataa
      181 gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc aacagttggc
      241 agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg caaaagcatt
      301 aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca tcgaaatccc
      361 aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt tgcaaaaagg
      421 aacgtcatta ttggaagaaa gttcttatcc gactggcggg aaaaacaccc atgccgttct
      481 ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta agttgaaaaa
      541 aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag tagaaaaaat
      601 ccaagtggtc ttacctgatg aagtagattc tttgggtatc caaaaaggca gagatctcgt
      661 gactttgctt acctgtactc cttatatggt caatacacat cgactgcttg tgacggggca
      721 tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg atcaatggaa
      781 aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg gcttagtttg
      841 gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc ctttatcttt
      901 ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg taaaaacgct
      961 caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt ctgatgaata
     1021 cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac atgaggagct
     1081 cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga aattgaaaaa
     1141 aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga aagaatgaat
     1201 gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa gctgacgaaa
     1261 tggacgtgta aactgttatt tttggtttac tatctggaac gccatcttct atttttttat
     1321 gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat aacatttgga
     1381 gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat acgtaccatt
     1441 tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag tttgttcatt
     1501 attttgctca ggagctgcgg cttgtgcctg ctgagaggcc tgttcagctg ctgattgctg
     1561 tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt ctgcttgttg
     1621 ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt cttcttgctt
     1681 tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag caagacggct
     1741 tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag ttgcatagtt
     1801 ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg tcactttttt
     1861 tctagtgcag ctttttccgc tttttgtttc tcttcttttt tcttttcaat ttgttcttgt
     1921 tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga agcctgatct
     1981 gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa acttcctgcg
     2041 accaaaaaca atgcaactaa ttttttcgct aaatttcctc cttaagtaaa aaacaaccgt
     2101 tttcaataaa tgtcct
//
LOCUS       DAZKMZ010000191         2065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000191 DAZKMZ010000000
VERSION     DAZKMZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.199"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8M5_002849"
     CDS             <1..156
                     /locus_tag="ACG8M5_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0360028.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8M5_002850"
     CDS             169..1077
                     /locus_tag="ACG8M5_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0360029.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACG8M5_002851"
     CDS             1077..1694
                     /locus_tag="ACG8M5_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360030.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1698..>2065
                     /locus_tag="ACG8M5_002852"
     CDS             1698..>2065
                     /locus_tag="ACG8M5_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360031.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          745 a          298 c          444 g          578 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcggt gtacaggttt
     1741 atccacaaac tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag
     1801 gcgatcctgg ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt
     1861 ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag
     1921 ataaaggtga tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg
     1981 ggttgatgtc gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa
     2041 atccaggtaa tgctacaact acgac
//
LOCUS       DAZKMZ010000192         2064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000192 DAZKMZ010000000
VERSION     DAZKMZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.200"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8M5_002853"
     CDS             278..820
                     /locus_tag="ACG8M5_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360032.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG8M5_002854"
     CDS             976..1425
                     /locus_tag="ACG8M5_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0360033.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2064)
                     /locus_tag="ACG8M5_002855"
     CDS             complement(1455..>2064)
                     /locus_tag="ACG8M5_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0360034.1"
                     /translation="AHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKK
                     WHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQT
                     IHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVH
                     WWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          741 a          325 c          386 g          612 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcg
//
LOCUS       DAZKMZ010000193         2063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000193 DAZKMZ010000000
VERSION     DAZKMZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.201"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..536
                     /locus_tag="ACG8M5_002856"
     CDS             3..536
                     /locus_tag="ACG8M5_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360035.1"
                     /translation="MSFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSIL
                     TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCS
                     NRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAF
                     SFAREDEDNPKTYDILA"
     gene            complement(597..962)
                     /locus_tag="ACG8M5_002857"
     CDS             complement(597..962)
                     /locus_tag="ACG8M5_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0360036.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1038..1283)
                     /locus_tag="ACG8M5_002858"
     CDS             complement(1038..1283)
                     /locus_tag="ACG8M5_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0360037.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1353..1607)
                     /locus_tag="ACG8M5_002859"
     CDS             complement(1353..1607)
                     /locus_tag="ACG8M5_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360038.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1771..2061)
                     /locus_tag="ACG8M5_002860"
     CDS             complement(1771..2061)
                     /locus_tag="ACG8M5_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360039.1"
                     /translation="MLFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMMLSY
                     KGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          759 a          352 c          367 g          585 t
ORIGIN      
        1 ttataagctt ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag
       61 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
      121 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
      181 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
      241 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
      301 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
      361 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
      421 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
      481 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
      541 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
      601 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
      661 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
      721 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
      781 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
      841 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
      901 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
      961 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
     1021 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
     1081 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
     1141 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
     1201 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
     1261 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
     1321 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
     1381 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
     1441 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
     1501 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
     1561 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
     1621 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
     1681 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
     1741 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
     1801 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
     1861 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
     1921 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
     1981 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
     2041 cgcaactaaa tcgaataaaa tcg
//
LOCUS       DAZKMZ010000194         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000194 DAZKMZ010000000
VERSION     DAZKMZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.202"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG8M5_002861"
     CDS             100..570
                     /locus_tag="ACG8M5_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0360040.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG8M5_002862"
     CDS             792..1895
                     /locus_tag="ACG8M5_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0360041.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKMZ010000195         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000195 DAZKMZ010000000
VERSION     DAZKMZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.203"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8M5_002863"
     CDS             <1..378
                     /locus_tag="ACG8M5_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0360042.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8M5_002864"
     CDS             426..1826
                     /locus_tag="ACG8M5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0360043.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKMZ010000196         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000196 DAZKMZ010000000
VERSION     DAZKMZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.204"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8M5_002865"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8M5_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8M5_002866"
     CDS             complement(728..1744)
                     /locus_tag="ACG8M5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0360044.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKMZ010000197         1908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000197 DAZKMZ010000000
VERSION     DAZKMZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.205"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8M5_002867"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8M5_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0360045.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8M5_002868"
     CDS             852..1040
                     /locus_tag="ACG8M5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0360046.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8M5_002869"
     CDS             1053..1610
                     /locus_tag="ACG8M5_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0360047.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8M5_002870"
     CDS             1642..1884
                     /locus_tag="ACG8M5_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0360048.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          699 a          295 c          392 g          522 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgtt
//
LOCUS       DAZKMZ010000198         1899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000198 DAZKMZ010000000
VERSION     DAZKMZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.206"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG8M5_002871"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG8M5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG8M5_002872"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG8M5_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          662 a          266 c          251 g          720 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaatta
//
LOCUS       DAZKMZ010000199         1896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000199 DAZKMZ010000000
VERSION     DAZKMZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.207"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1137
                     /locus_tag="ACG8M5_002873"
     CDS             10..1137
                     /locus_tag="ACG8M5_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0360049.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1355..>1896)
                     /locus_tag="ACG8M5_002874"
     CDS             complement(1355..>1896)
                     /locus_tag="ACG8M5_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0360050.1"
                     /translation="EEFYKRLMRFIFVTGVRSEELLALQWKDFDLINGRCSVNKSIYI
                     RTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSIGKNIELVFSFDGLPPSPR
                     TILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYAISKRLGHASIKTTLDKYG
                     HLYPETNQALANEITRFDI"
BASE COUNT          647 a          336 c          336 g          577 t
ORIGIN      
        1 gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcga agatatcaat
       61 gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc agaggccgaa
      121 gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt tttaggtacg
      181 gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa actaaatcta
      241 aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa cggttattta
      301 ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat caatgctgaa
      361 tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct taagaatgcg
      421 ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct gtatgtaaaa
      481 ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat ttctcttgag
      541 gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat tcctttgaat
      601 attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg ggatgatgtc
      661 gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga cggcgcatgg
      721 gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg acaaaccttg
      781 ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa gtatggaaag
      841 ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt tacgccaagt
      901 gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt taacttccat
      961 tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat gaaagaaatc
     1021 tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc gcatgtaaca
     1081 gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa ttcttgattt
     1141 ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa acatggttta
     1201 ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct cgtccatttt
     1261 cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata aaaataggct
     1321 atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc gtaatttcat
     1381 ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa gtagttttaa
     1441 tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt tcaattaaaa
     1501 aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct gcaatttcag
     1561 ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca tcaaaagaaa
     1621 aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt tttaaatctt
     1681 caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt gtttcagcaa
     1741 attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat ctgccattta
     1801 ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca cctgtaacaa
     1861 aaataaaacg catcaaacgt ttataaaatt cttcat
//
LOCUS       DAZKMZ010000200         1872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000200 DAZKMZ010000000
VERSION     DAZKMZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.208"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8M5_002875"
     CDS             73..234
                     /locus_tag="ACG8M5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0360051.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8M5_002876"
     CDS             253..1530
                     /locus_tag="ACG8M5_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0360052.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..>1872
                     /locus_tag="ACG8M5_002877"
     CDS             1540..>1872
                     /locus_tag="ACG8M5_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0360053.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADES"
BASE COUNT          773 a          172 c          308 g          619 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gt
//
LOCUS       DAZKMZ010000201         1815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000201 DAZKMZ010000000
VERSION     DAZKMZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.209"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8M5_002878"
     CDS             5..403
                     /locus_tag="ACG8M5_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0360054.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8M5_002879"
     CDS             387..1082
                     /locus_tag="ACG8M5_002879"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0360055.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..>1815)
                     /locus_tag="ACG8M5_002880"
     CDS             complement(1676..>1815)
                     /locus_tag="ACG8M5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HFQ0360056.1"
                     /translation="YKSELEKIATEAKTLDTEFMDKTIRKVMKKDNVTIKDPYVKNIF
                     K"
BASE COUNT          664 a          265 c          348 g          538 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatt
//
LOCUS       DAZKMZ010000202         1743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000202 DAZKMZ010000000
VERSION     DAZKMZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.210"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1691)
                     /locus_tag="ACG8M5_002881"
     CDS             complement(225..1691)
                     /locus_tag="ACG8M5_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0360057.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          551 a          355 c          308 g          529 t
ORIGIN      
        1 aaacaaggga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa atggtgttca
       61 atcagctgac cttcagcatt agctcaaaaa acggcaaaat atgaggagct atttttgttt
      121 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
      181 agaagtagct tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact
      241 gtttctggtt cagtaatttc tttgtttttg tctcgacgag caattgctgg caagatcata
      301 cctaataaga tcaagacaat tggtgtgatg atattcgtga ataattggaa ccaccatgct
      361 ttaggatcag cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg
      421 aaggcaaagc accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat
      481 tcagaattga atttcttata agctttattc aacatcatat atgccaagaa cacccatagg
      541 taacgcattg gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata
      601 tctttgatac caaagatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga
      661 taaccattca ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct
      721 ggatccgcat ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag
      781 atttgtccaa tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag
      841 taattaccta aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttagggata
      901 ttatcgctag caaacagcat acccattgcg actgagccta atacagcaca aatccctacc
      961 attgctgcta agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg
     1021 acataagggg atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg
     1081 aaataactga aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca
     1141 aattccatcg ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac
     1201 atgacgaaca tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt
     1261 gttgataaat ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact
     1321 gacatcgtat ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt
     1381 gcttgaggct tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag
     1441 tatgccagtc gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta
     1501 tccttgaagg ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc
     1561 aaaatcaata gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca
     1621 ttccccatgc cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt
     1681 ttgttgttca ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt
     1741 caa
//
LOCUS       DAZKMZ010000203         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000203 DAZKMZ010000000
VERSION     DAZKMZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.211"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8M5_002882"
     CDS             <1..156
                     /locus_tag="ACG8M5_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0360058.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8M5_002883"
     CDS             169..1077
                     /locus_tag="ACG8M5_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0360059.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACG8M5_002884"
     CDS             1077..1697
                     /locus_tag="ACG8M5_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360060.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DAZKMZ010000204         1726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000204 DAZKMZ010000000
VERSION     DAZKMZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.212"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..1119)
                     /locus_tag="ACG8M5_002885"
     CDS             complement(100..1119)
                     /locus_tag="ACG8M5_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0360061.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1116..1340)
                     /locus_tag="ACG8M5_002886"
     CDS             complement(1116..1340)
                     /locus_tag="ACG8M5_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0360062.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1337..1630)
                     /locus_tag="ACG8M5_002887"
     CDS             complement(1337..1630)
                     /locus_tag="ACG8M5_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360063.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          484 a          354 c          291 g          597 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcatt tgtttcctcc ttcttcgctt
     1141 tcagccgaga acattttgta ggttcgattt gatacaccca acacactccc taaaaacgcg
     1201 ccaaaaccag taatgatgac aacacagata tctgtgtact gccaattgag cgctttacca
     1261 actaacccca cgaaagtagc tagtgcggga ataattacca gtgcgaacca ttttagtact
     1321 tcgaacgttt tattattcat tttcttctct ccctaaacaa agttttgatt tgttgcgtgt
     1381 gttccaccaa tttttctgta tgtgtatcta atctttcatc gtgtttcttt agttcttcat
     1441 gaatcatcaa tcgatctgat ttgctcgatt ctaaatcttt agtcagcaaa tctaaattgt
     1501 ggcttacttt tgaaagagtc tcagtaattt tcgagaaaga tgcagcaatc ggtctaatta
     1561 ctaataaaat caaagaaacg atagcggtta ttgatcctgc tatcattccc cattccccta
     1621 aattaatcat gtgacaactc ctttacctta aataaaaacg catcaattaa gatgcgctct
     1681 tatctttatt aatgatttta tctgcttctt cgtctgtaat gcataa
//
LOCUS       DAZKMZ010000205         1718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000205 DAZKMZ010000000
VERSION     DAZKMZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.213"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..1643)
                     /gene="cls"
                     /locus_tag="ACG8M5_002888"
     CDS             complement(195..1643)
                     /gene="cls"
                     /locus_tag="ACG8M5_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0360064.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          514 a          370 c          290 g          544 t
ORIGIN      
        1 gctttgaaat gtgtctcttt taatagcata tattagctta tttcttgctt tccccaaaaa
       61 acaagagata ccccaaagtt aatttcgctc atgcgagacg ctttctccat ttctgacgta
      121 ctctcagaaa tggccccgtt agaaaaggac acgagatctc catctgttga tggttctcat
      181 gtccttttta tgttttaaag gagcggcgaa atcaatcttg ctaatccttc tttcatcttg
      241 atgattattc ctcggttttc atacatttct tttgtgagat gcgtagaaat tttcgagtct
      301 tcaaaaaaag cttttcttac acgtgaagcc attcgttcat cataaaccag tgtattgacc
      361 tcaaagtcca agcgaaatga acgaacatcg atatttgctg agccaactga aacaatgcca
      421 ccatcgataa tcatcgtctt tgcatgaata aatccattct catatgtttc gatccttgct
      481 ccatactcga tcagctctgc agcgaaagaa tacgttgccc agtaaacgag aagatgatct
      541 ggtttgttag gtatttggat atgaactttt acgcctgaaa gaagagccaa cttcagtgct
      601 tcatgaatcg atccatccgg tatgtaataa ggcgtttgga tcaatatctc ccgtttggct
      661 aacgaaatca ttttcaaata agtcattttg atttgttcat gttcggtatc tggtccgctt
      721 gtcacgattt gaacagctat atcaccagta gtgtgaatgg acgggaaaaa tttgggagaa
      781 tagcctactt cttttgtatg ctgagaattc cagtccatca aaaaacggtt ttgcaaggta
      841 tacaccgctt ctccgtaaat acgcaaatga ttgtctcgcc aataaccaaa tttctttacc
      901 aatcctagat attcatttcc aacattgaac ccacctgtat atccgatttt cccatcaata
      961 actacgattt tacgatgatt gcgataattg atccgaggat taatatacgg aacaaataga
     1021 ggaaagaaaa atgcgacttc tccgcctgct tttttcaaat tctgaaaaaa gctggaagaa
     1081 acttgtgtgg aaccccatgc atcaagaagg actctgactt ttacccctcg tcttgccgcc
     1141 tctgtcaatg catctcgaac ttcttcacct aatgcatcac tgcgataaat ataatactgg
     1201 aagtggatat ggtcttctgc ctgcttgata tcttcaatca gcgcatcgaa tttttcacga
     1261 ccatctgtaa ataaagtgat ttcattttta gtcgtataga gcgtactttc aaataccgtg
     1321 agcatataga tcaattgttt cacatctact tgtccagtag gcggatgagg ataaagccct
     1381 cgcatcaatg cctgtttgtc ttcttcaatt tccaggttct taccgatttt cccttggatc
     1441 ttcaaatcaa agattttctc tttggaaatc cctcttccta aaaaaatata taaaataaat
     1501 cctacaactg gaatgaacat caaaacgagc agccatgccc atgtttgagc agtttccttt
     1561 ctttctcgaa aaacgatgat aaaggaaagc aaaatattca taatcaaaag gatcgtaaaa
     1621 aaattgtcaa cgataatttt cacggcttca cctcttcttc taccaactta agaataccca
     1681 agttgttttt taaagtaaag ggaaaaaagc cgcttcct
//
LOCUS       DAZKMZ010000206         1695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000206 DAZKMZ010000000
VERSION     DAZKMZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.214"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8M5_002889"
     CDS             <1..1412
                     /locus_tag="ACG8M5_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0360065.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1695
                     /locus_tag="ACG8M5_002890"
     CDS             1546..>1695
                     /locus_tag="ACG8M5_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFQ0360066.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIK"
BASE COUNT          670 a          254 c          363 g          408 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaa
//
LOCUS       DAZKMZ010000207         1681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000207 DAZKMZ010000000
VERSION     DAZKMZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.215"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..942
                     /locus_tag="ACG8M5_002891"
     CDS             292..942
                     /locus_tag="ACG8M5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360067.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            1129..>1681
                     /locus_tag="ACG8M5_002892"
     CDS             1129..>1681
                     /locus_tag="ACG8M5_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0360068.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGV"
BASE COUNT          543 a          201 c          343 g          594 t
ORIGIN      
        1 ataactggag attaattaaa aactggaagg aactgctcga tcaaccttga gaactgtttt
       61 tcttacttct ggtcaagatt ctcgtggttg gtggataagg atggttgata agttcattgg
      121 attagctagt cgaaaacaac caggtgtaat taatgtagaa gttttaacaa ggggatggag
      181 tgatttattc agtgataatc ataagttcat aaatagtaat tgggatgtat ggttggtata
      241 cgttatacct gaacttgaac tatcaagatt tggtttagaa tttgagaatc gatgtttata
      301 aagggagggc aaaaaatgtg cttttttaca acttttacta tcattatgat tgcagtactt
      361 tttatttttc tagatatagc aaagcgaaat acagcatttt tattatatcg ggttttactt
      421 cgagcaggtc tcataacatt tatatcgatc gttggatttt ttctattcac agttattgta
      481 tttatttgga gaactacagc accaccattg ccagagatta catatggaga atttccgttt
      541 agtcttgaat atgaaataga tgaggaaaga attttgattg aagatacggt gattgcagaa
      601 tttataggaa gttttagagg gaatgtgaca actcaaccta gacgaagttg gagtataaaa
      661 cttttaaata atagtggaga gttgaaagca tttcattatg aattcttaat aatagaaagt
      721 gaagatgtaa caatcacttt aagtagtggc tctgctggat attttatgaa tgatggtgga
      781 agcaagccac agctcacagt ccgtgatcgt attgcaggtt ttatgcattt ttctcactat
      841 gacttagaag aagctcgtaa aatacttgca gaatatggga tcacattaat caattgggaa
      901 tatgcgccac cgataagaaa ttttttcaat gattattatt agtcttataa aaaacagaat
      961 gtaaaattta tgtgcgcgag tatttagttt tccatttttt gtttgataga tatatagcat
     1021 tgttgaagca tgggatagtg gtgccaattt tatcaatgag gttggtcagg cagttaacat
     1081 aattggtgat gctataaatc catttaattg gaattggagg aattaggtgt ggaaacagtg
     1141 agattatgga tattcatgaa aaattttcgt tcggctttga gatatgttat tctggaatca
     1201 aaatcaggtt attatctgat tgacacagat cactatactt tttggggagg ctaccttttt
     1261 ccgattttta attggataat cccccataag gttatgaaaa ttaacttgcc tgcagaagaa
     1321 ataaatagat taatactcaa caaagattta caaaggaaaa gagagaaaga acattctaat
     1381 gttgcttgga taggtccagc atttgcagta gtgacttctt caattgtcgg tcctatgcta
     1441 gagagtattt atagtgattt ttctattgga atacgttatt tatttgttac tttaagtata
     1501 gctgttcttt tgtgcataaa gggttatgtt agcaagaaga ataaagatat tttaacacta
     1561 atcggtgtaa acaatttatc tagtactaaa attatactgt ttcctgaatc tataaaagaa
     1621 ggatcaaggt tgttgataat ttttataatg cttatactgt tttctgtttt atttggggtg
     1681 a
//
LOCUS       DAZKMZ010000208         1647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000208 DAZKMZ010000000
VERSION     DAZKMZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.216"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8M5_002893"
     tRNA            complement(20..91)
                     /locus_tag="ACG8M5_002893"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8M5_002894"
     tRNA            complement(113..200)
                     /locus_tag="ACG8M5_002894"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8M5_002895"
     tRNA            complement(225..298)
                     /locus_tag="ACG8M5_002895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8M5_002896"
     tRNA            complement(312..382)
                     /locus_tag="ACG8M5_002896"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8M5_002897"
     tRNA            complement(384..459)
                     /locus_tag="ACG8M5_002897"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8M5_002898"
     tRNA            complement(478..551)
                     /locus_tag="ACG8M5_002898"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8M5_002899"
     tRNA            complement(564..653)
                     /locus_tag="ACG8M5_002899"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8M5_002900"
     tRNA            complement(677..750)
                     /locus_tag="ACG8M5_002900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8M5_002901"
     tRNA            complement(764..837)
                     /locus_tag="ACG8M5_002901"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8M5_002902"
     tRNA            complement(865..937)
                     /locus_tag="ACG8M5_002902"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8M5_002903"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8M5_002903"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG8M5_002904"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG8M5_002904"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG8M5_002905"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG8M5_002905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG8M5_002906"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG8M5_002906"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG8M5_002907"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG8M5_002907"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG8M5_002908"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG8M5_002908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG8M5_002909"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG8M5_002909"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG8M5_002910"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG8M5_002910"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZKMZ010000209         1606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000209 DAZKMZ010000000
VERSION     DAZKMZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.217"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACG8M5_002911"
     CDS             <1..483
                     /locus_tag="ACG8M5_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0360069.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            520..1530
                     /locus_tag="ACG8M5_002912"
     CDS             520..1530
                     /locus_tag="ACG8M5_002912"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0360070.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          572 a          253 c          347 g          434 t
ORIGIN      
        1 atgattgact tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat
       61 cgagccattt atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt
      121 acagagacca aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact
      181 tttactacga aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct
      241 attgtttcat cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg
      301 tttggttatg aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa
      361 cttgtttttg caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac
      421 aaaatcatcc agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat
      481 taaccaagta aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta
      541 gaaagaaatg caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat
      601 tcgttactcg aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga
      661 aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa
      721 ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa
      781 ttattgattg agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta
      841 ggttattcag atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact
      901 gcacatggaa ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg
      961 tgggaaagtg tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag
     1021 tatcaaaaag tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca
     1081 acaaattggc aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt
     1141 tgtattgata cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa
     1201 agaaagtata atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca
     1261 gttgatttaa gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg
     1321 gggatcatgt tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta
     1381 tacgaagatc tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat
     1441 gtaccgccac aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga
     1501 aaagcgactg ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa
     1561 agaccaataa atctaaatag aaaagaaagt gaaagaagct gacaaa
//
LOCUS       DAZKMZ010000210         1589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000210 DAZKMZ010000000
VERSION     DAZKMZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.218"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1493
                     /locus_tag="ACG8M5_002913"
     CDS             <1..1493
                     /locus_tag="ACG8M5_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0360071.1"
                     /translation="VGLLTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLAICC
                     ILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSMNWFSVLWKKFIGGVLSVGAGLFL
                     GREGPSIQLGASIGQGAGQLFRSPSSEEKILISSGASSGLAAAFNAPIAGLLFVLEEI
                     HHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLLGI
                     LGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFGHA
                     SLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDPIY
                     IKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGGNP
                     IYESLLERLLGNTQTASLKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRRGN
                     QEILTHGDTIMNVGDILVILTDEANLPRIKQEIEAKSSITNLKQSF"
BASE COUNT          407 a          305 c          341 g          536 t
ORIGIN      
        1 ttgttggact gctgacagga atcgtcgtta gcctgtttcg attatctatc gaaaagctaa
       61 gcgatttagt tcgaagtatc tatgaaatga gtcgggaaca gccgatttac ttaattggcc
      121 tagctatttg ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa aatgaaccgg
      181 atatcaaggg aagcgggatt ccacaagtag tgggacagct tcgcggggaa ttatcaatga
      241 actggttctc tgttttatgg aaaaagttca ttggcggtgt actttcagtt ggtgcgggcc
      301 tttttctagg tagagaagga ccttctatac agcttggcgc atccattgga caaggagctg
      361 gacaattgtt tcggagtcca tcttcagaag aaaagatttt aatttctagc ggtgcaagta
      421 gcggacttgc agcagcattc aatgctccta tcgcaggatt actctttgtt ttagaagaaa
      481 tccatcactc cttttctcca cttgtttggc tgacatcatt tgcttcagca atcactgcta
      541 acttcgtctc tctttacttt ttcggcttga gacctgtttt atatctaggt gaccttcctt
      601 ctttgccgtt gaaatattat ggttccttag tgatcttagg agtacttcta ggcatcttag
      661 gttttattta tcagaatgtc ttgctggctt tgccaaaatt ttacaaccga ttgcctttac
      721 cagcttattt tcacgggttc gttcctttct tgctgatttt gccaattggt tatttatggc
      781 ctagtactct aggcggaggc aatcaaatcg ttttagcttt tggacatgct tccttgccaa
      841 tcattatgat tatcggtttg tttgttcttc gattcgtttt ttccatgatt tcttacggtg
      901 ggggtcttcc aggggggatt ttccttccga tattgacatt gggggcttta atcggtagct
      961 tttatggaaa cattatggtt gatttcggga tggatccaat ctacatcaag gattttgtcg
     1021 ttattgccat ggctggttat ttcacagcga ttggaaaagc accgctgaca gcaattattt
     1081 tagtgacaga aatggttggc tctcttaatc atctgatgcc tttaggattg gctgctcttg
     1141 ttgcttatat cgtcaatgac cttctcggcg gtaatccaat ttacgaatca ttattggaac
     1201 gtctacttgg aaatactcag accgcctcac taaaaggtgc aaggacaact tttgattttc
     1261 cagtaacggc agagagttct ttagacggat taatggtacg agacttcaat tggcctaaaa
     1321 atatgctgct aatctctata cgcagaggaa atcaggaaat cttgactcac ggtgatacga
     1381 tcatgaatgt aggagatatt ttggttatct tgactgatga agcaaacttg ccaagaatca
     1441 agcaggaaat cgaggctaaa tcttctatca caaatttgaa gcaatcattt taacgaacat
     1501 ggaaaaacta ttttacaaca actattcatc tacactactt gattctatat gattctataa
     1561 aaaaagaaga ggctgggaaa acccagcct
//
LOCUS       DAZKMZ010000211         1577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000211 DAZKMZ010000000
VERSION     DAZKMZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.219"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG8M5_002914"
     CDS             <1..189
                     /locus_tag="ACG8M5_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0360072.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..>1577)
                     /locus_tag="ACG8M5_002915"
     CDS             complement(1055..>1577)
                     /locus_tag="ACG8M5_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0360073.1"
                     /translation="GLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITRKSR
                     GKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKNAVL
                     DIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNNKIV
                     IGKHCWIGAKSVI"
BASE COUNT          491 a          311 c          190 g          585 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccg
//
LOCUS       DAZKMZ010000212         1570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000212 DAZKMZ010000000
VERSION     DAZKMZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.220"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(354..1472)
                     /locus_tag="ACG8M5_002916"
     CDS             complement(354..1472)
                     /locus_tag="ACG8M5_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360074.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT          457 a          347 c          251 g          515 t
ORIGIN      
        1 tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag aaataatttt
       61 tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct tccttaaacg
      121 gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat gaagagctat
      181 ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctcatacacg
      241 gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg tgaaactatt
      301 tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct cttttaataa
      361 cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa aataaacact
      421 ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg ccgatcatat
      481 tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat aaataaatgt
      541 gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat gaatttctta
      601 tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga cagcttcttc
      661 gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat cgcaacgtct
      721 gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc tggtaatgca
      781 aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta cgtatcaaca
      841 gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt tacgccatgg
      901 aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa accgccacct
      961 gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg tagagatact
     1021 tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt caatcccaat
     1081 aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata aggaagatca
     1141 cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt ctggtcagca
     1201 gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc tcctaacaaa
     1261 ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt ctcaaaacgt
     1321 ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac atcttgatgg
     1381 gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc tgaagtcaat
     1441 tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa aaaatcattc
     1501 ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa acgtatctaa
     1561 aaaaataaaa
//
LOCUS       DAZKMZ010000213         1556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000213 DAZKMZ010000000
VERSION     DAZKMZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.221"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            332..1438
                     /locus_tag="ACG8M5_002917"
     CDS             332..1438
                     /locus_tag="ACG8M5_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0360075.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          437 a          255 c          339 g          525 t
ORIGIN      
        1 tgttaggtga ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa
       61 tgggtaaaac agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta
      121 cgttgtatag tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa
      181 aaggtttctg accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc
      241 tcctcacctg acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg
      301 gtggattttg ggtccttatt tgactttgtc catgatcggt cttttagaag tatacagtgc
      361 cagttcttat cgtttattgc aagccgatga aaatacaaaa agtctattat tgcgtcaact
      421 aattttcata tttttgagtt ggagcgtgat cttcttagct cgttcagtca aactacacta
      481 tttacttcac cctaaaatag caggatacgg tttagcctta tcgattttct ttttagtatt
      541 agtaagaata gggatattcg gtgtcactgt caacggcgca caacgttgga tctctctgtt
      601 tggcattcaa ttccagcctt ctgaactggc aaatcttttt ttgatttttt atttaagctg
      661 gttttttcgt gacggaaata gtagcccaaa agatctaaaa aaaccattcc tgattacagt
      721 aggtataact tttctgattt tatttcagcc aaagattgct ggagcattga tgatcctttc
      781 gattgcgtgg gtcatatttt gggcagcggc ggttccattt aaaaaaggga tctatctaat
      841 cgttactttt tctgcattgc tgattggagc agcaggcggg gtattatatt taggaaataa
      901 aggttggctt ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt taagagattc
      961 ttttatcgat agtcatggtg cgggctatca aatgacccat tcgttttatg cactatataa
     1021 tggtggcata tttgggcggg gattaggaaa tagtatcaca aaaaaagggt atcttccaga
     1081 gacagaaaca gattttattt tttcgattat tacagaagag cttgggctaa tcggtgcatt
     1141 gtgtgtgctt ttcttattgt tctcattatg catgagaatc ttttgtttaa gcagtcgctg
     1201 taaaaatcag caagctggat tgtttctttt aggattcgga actttgctat tcgttcagac
     1261 gataatgaac gtgggaagta tcgcggggct gatgccaatg actggtgtgc cattgccatt
     1321 tgttagctac ggaggaacaa gttatctgat cctttcttta ggaatcggaa tcacgttgaa
     1381 tatttcttca aagatacagg ccgaagagtt ggcgctctat cgacctgaaa aacaataaag
     1441 agaaactgag aaactctcag tttgaattat cataaagtac aatcttttat ttttcaagcg
     1501 agaggttctt ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc atatta
//
LOCUS       DAZKMZ010000214         1541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000214 DAZKMZ010000000
VERSION     DAZKMZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.222"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8M5_002918"
     CDS             complement(52..1518)
                     /locus_tag="ACG8M5_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0360076.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          504 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa a
//
LOCUS       DAZKMZ010000215         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000215 DAZKMZ010000000
VERSION     DAZKMZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.223"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            788..1261
                     /locus_tag="ACG8M5_002919"
     CDS             788..1261
                     /locus_tag="ACG8M5_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0360077.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1270..>1479
                     /locus_tag="ACG8M5_002920"
     CDS             1270..>1479
                     /locus_tag="ACG8M5_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0360078.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSN"
BASE COUNT          485 a          243 c          228 g          523 t
ORIGIN      
        1 gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat ttggtatcag
       61 taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca tcttcgtatt
      121 tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg cacatgtttt
      181 aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct tcttaatacc
      241 tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat taagctattt
      301 cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat aacattgtat
      361 aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga aaaattctga
      421 tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc
      481 ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata aaaaatcctc
      541 caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa gagacacaca
      601 tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca
      661 ctccacacct tcatactcta tcatttttta taaacgatat atttttattg tttttcgata
      721 aaaaatagtt gttttacaat aattattctg ctatatttta gctatcaaag ataaaagagg
      781 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
      841 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      901 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      961 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
     1021 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
     1081 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
     1141 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
     1201 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
     1261 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
     1321 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
     1381 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
     1441 caaccaggtg atttgttgac ctataaaaat gactcgaat
//
LOCUS       DAZKMZ010000216         1458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000216 DAZKMZ010000000
VERSION     DAZKMZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.224"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M5_002921"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M5_002921"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8M5_002922"
     CDS             complement(580..1353)
                     /locus_tag="ACG8M5_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0360079.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
BASE COUNT          447 a          284 c          272 g          455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaatt
//
LOCUS       DAZKMZ010000217         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000217 DAZKMZ010000000
VERSION     DAZKMZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.225"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8M5_002923"
     CDS             complement(150..1337)
                     /locus_tag="ACG8M5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0360080.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKMZ010000218         1413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000218 DAZKMZ010000000
VERSION     DAZKMZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.226"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..725
                     /locus_tag="ACG8M5_002924"
     CDS             474..725
                     /locus_tag="ACG8M5_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360081.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="ACG8M5_002925"
     CDS             759..1037
                     /locus_tag="ACG8M5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360082.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          504 a          195 c          264 g          450 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gat
//
LOCUS       DAZKMZ010000219         1387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000219 DAZKMZ010000000
VERSION     DAZKMZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.227"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..365)
                     /locus_tag="ACG8M5_002926"
     CDS             complement(75..365)
                     /locus_tag="ACG8M5_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360083.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(483..743)
                     /locus_tag="ACG8M5_002927"
     CDS             complement(483..743)
                     /locus_tag="ACG8M5_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360084.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(880..1293)
                     /locus_tag="ACG8M5_002928"
     CDS             complement(880..1293)
                     /locus_tag="ACG8M5_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0360085.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          508 a          234 c          205 g          440 t
ORIGIN      
        1 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
       61 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      121 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      181 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      241 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      301 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      361 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      421 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      481 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      541 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      601 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      661 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      721 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      781 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      841 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      901 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
      961 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1021 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1081 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1141 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1201 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1261 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1321 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1381 gctttgt
//
LOCUS       DAZKMZ010000220         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000220 DAZKMZ010000000
VERSION     DAZKMZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.228"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..>1364
                     /locus_tag="ACG8M5_002929"
     CDS             80..>1364
                     /locus_tag="ACG8M5_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0360086.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DAZKMZ010000221         1353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000221 DAZKMZ010000000
VERSION     DAZKMZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.229"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1353
                     /locus_tag="ACG8M5_002930"
     rRNA            <1..>1353
                     /locus_tag="ACG8M5_002930"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          359 a          298 c          416 g          280 t
ORIGIN      
        1 tttgagcaat ggagggacgc aggaggctaa ggaatgcaga cgatcggaaa tgtctgtcca
       61 agcagtaagt ctgaagagga gtcaaatgct tcttttctta aggacaagct gtgatgggga
      121 gggaaataat agtaccgaag ttcctgatgt cacactgccg agaaaagctt ctagtgagaa
      181 aacagcggcc cgtaccgcaa accgacacag gtagtcgagg agagaatcct aaggtgagcg
      241 agagaactct cgttaaggaa ctcggcaaaa tgaccccgta acttcgggag aaggggtgct
      301 gatcatacga tcagccgcag tgaataggcc caagcgactg tttatcaaaa acacaggtct
      361 ctgcaaaatc gtaagatgaa gtataggggc tgacgcctgc ccggtgctgg aaggttaaga
      421 ggagtgctta gcgcaagcga aggtacgaat tgaagcccca gtaaacggcg gccgtaacta
      481 taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacccgca cgaaaggcgt
      541 aacgatttgg gcactgtctc aacgagagac tcggtgaaat tttagtacct gtgaagatgc
      601 aggttacccg cgacaggacg gaaagacccc atggagcttt actgtagttt gatattgagt
      661 gtctgtaccg catgtacagg ataggtagga gccgtagaaa tcggaacgct agtttcgatg
      721 gaggcgctgg tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt
      781 ggtgggagac agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg
      841 gaggcgccca aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa
      901 gggagcttga ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg
      961 gtggttccgc atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc
     1021 ttatctcccc caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg
     1081 catcctgggg ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg
     1141 agctgggttc agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt
     1201 tgagaggagc tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt
     1261 gttctgccaa gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct
     1321 aagtgtgaag cccacctcaa gatgagattt ccc
//
LOCUS       DAZKMZ010000222         1340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000222 DAZKMZ010000000
VERSION     DAZKMZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.230"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..897
                     /gene="erm(B)"
                     /locus_tag="ACG8M5_002931"
     CDS             160..897
                     /gene="erm(B)"
                     /locus_tag="ACG8M5_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0360087.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1067..1327
                     /locus_tag="ACG8M5_002932"
                     /pseudo
     CDS             1067..1327
                     /locus_tag="ACG8M5_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          513 a          222 c          219 g          386 t
ORIGIN      
        1 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
       61 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      121 gtatttgttt atagatgtaa tcacttcagg agagattaca tgaacaaaaa tataaaatat
      181 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      241 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      301 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      361 tcagaaaaat taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt
      421 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      481 caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct gattgttgaa
      541 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      601 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      661 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      721 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      781 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccattact
      841 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
      901 tatgagtcgc ttttttaaat ttggaaagtt acacgttact aaagggaatg gagataaatt
      961 attagatata ctactgacag cttccaagaa gctaaagagg tccctagcgc ctacggggaa
     1021 tttgtatcga taaggggtac aaattcccac taagcgctcg ggaccccttg taggaaaatg
     1081 tcctaagtgt ggcaacaata ttgtattaaa aaaatcgttt tatggttgtt caaattatcc
     1141 tgaatgtaag tttactttag ctgaacattt tagaaagaaa aaactaacca aaacgaatgt
     1201 aaaagaatta ctggagggaa aagaaaccct ggtaaaagga atcaaaaaca aagagaaaaa
     1261 gccctacaat gccgttgtaa aaattgggga aaagggatat attgatttta tctctttttc
     1321 aaaataaaca taaaagccct
//
LOCUS       DAZKMZ010000223         1322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000223 DAZKMZ010000000
VERSION     DAZKMZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.231"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8M5_002933"
     CDS             85..624
                     /locus_tag="ACG8M5_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360088.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          499 a          169 c          206 g          448 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ct
//
LOCUS       DAZKMZ010000224         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000224 DAZKMZ010000000
VERSION     DAZKMZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.232"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1218
                     /locus_tag="ACG8M5_002934"
     CDS             <1..1218
                     /locus_tag="ACG8M5_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0360089.1"
                     /translation="EEWLIEKEYKGVQAQFIKGRLDDSPSHCEHCGSIRIIRNGSYTT
                     RTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYEN
                     QSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMKSVSGSMSFIC
                     VDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAE
                     IVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPL
                     KRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRDTEGFQETLKTIS
                     STLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIAFGYRNFYNFR
                     ARIYLQQGLIFEN"
BASE COUNT          399 a          274 c          254 g          357 t
ORIGIN      
        1 gaagaatggc tgattgaaaa agaatataaa ggcgttcaag cgcagtttat taaagggaga
       61 ttggatgact caccttccca ttgtgaacac tgtggatcga ttcgtattat ccgcaacggt
      121 tcctatacga cacgcactca gatgttgaaa gtcaaagaaa agttgactat tttagaatta
      181 aaacgtaccc gttttctctg ccatgattgt ggacggacct tttctgcgaa aacggactta
      241 gttgatgaac atcatcaact aacaaaggaa ctgaagcagg cgattctcat ggaactgtac
      301 gaaaatcaat cacgtaaact aatcgctaag aagtacttcg tctcggatgg gacggtgaca
      361 cggatcttgc gtgaagcaac gaagcattac cagccacgta tgaacttcct tccgacggtg
      421 ttgtgtatgg atgagttcaa atcaatgaag tctgtttctg gttccatgag ttttatttgt
      481 gttgatggga caaccaatca gctatttacg atccttgaag atcgcagact ttacaaacta
      541 actcaatact tcctgcgctt tcctagaaag gcccgtttaa aggtcaagta cctagtgatg
      601 gacatgaatg ccagctattg tcagttgctt aaaacagtct ttccaaatgc ggagatcgta
      661 actgaccgct ttcatattgt ccaacacatc aatcgcagct tcaatcaatt acgtgtccag
      721 atcatgaatc gttttcgaac ctcacactct gaaaatcaga agaagtaccg tcggctcaag
      781 cgatattgga aactcttact caaagattcc acgacattgg agccactcaa acggcattat
      841 catcgactgt ttaaacgacc aattagtcaa acagaaatcg ttgatgagct gttaagctac
      901 aacgaagaat tgcgaactgc gtatcatttt tgtcaattat tacgctatta tttcgttaaa
      961 cgagatacgg aaggctttca agaaaccctt aaaacaattt catctacgtt acctcagtcg
     1021 tttaaaaaga aattcacgat cttccatcgc taccaatcgg gtatttcaaa tgcctttaag
     1081 gtgccccact caaacggcgt cactgagggg ctgaataaca agatcaagct gattaaacga
     1141 attgcgtttg gttatcgcaa cttttataac tttcgggcac ggatctattt acaacaaggt
     1201 ctgatctttg aaaactaaaa aaatatgcgg cgcacagaac gcgccgcata tcggactaag
     1261 ttttattcaa atttgtcttc gcca
//
LOCUS       DAZKMZ010000225         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000225 DAZKMZ010000000
VERSION     DAZKMZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.233"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..842
                     /locus_tag="ACG8M5_002935"
     CDS             <1..842
                     /locus_tag="ACG8M5_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0360090.1"
                     /translation="SIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLASELG
                     IADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESS
                     ISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHS
                     TDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAK
                     LGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISE
                     IKE"
BASE COUNT          429 a          211 c          213 g          431 t
ORIGIN      
        1 tctccataag actacaaatc aaaacaatgt acaacgactt cagcccaaaa gaacaggcta
       61 tcgccgatta tattttagaa aatccaagca aagtaagtca tagtcctatt agcaatttag
      121 caagtgaact gggcattgct gactctactt tttttcaatt tacaaaaaaa cttggttata
      181 atggatttaa agattttaaa atggccatgt tgatccaaga aaatgatcta tctgctattt
      241 ctatccacga aaatattcag aaaacagata ctgagctcac tatggcgcaa aaggtgttcg
      301 agtctagtat ttcaacttta aatgacacaa aaaagttgtt acaacaaaag gatctaaaaa
      361 aagcggctac tattatcaat gaatctaaac gtgtctattt tttcggtgtt ggcggttcag
      421 agattgtagc aactgacgct tatcataaat ttttgcgttc ccctatcgca acttctcaca
      481 gcacggatta ccatattcaa ttgatggaag cttctttatt gacggaaaaa gactgtgctg
      541 ttttgatttc tcacacaggg caatcaaaag aaacaatcca tattgctgaa accgtaagaa
      601 aaactggagc aaaaacaatc gttattacta gccaagctaa ttcttccctt gctaaacttg
      661 gcgatgtcgt ttttatttct atttctgaag aaacagaatt ccgatctgaa gctttagctt
      721 cccgtatttc tcaactaagc attttggact ctctatatgt gattttgatg ttttacaacg
      781 aaaaagaatc acgtcgttct atcgctaaag ttcgacatac tatttctgaa atcaaagaat
      841 agcaccataa cagaaaaagc gaaaaatcca taaaacaaaa aaagccacta ccaaatattt
      901 ggtagtggct tttttggttc tgcaagggtg gtacagaaca tcaatagtcg gtgggttaga
      961 acgtatgtca tcgcatcttt ggagaaggtt gtattggtat tattcgagtt acttgttaga
     1021 tgcgataaca aagttagtat acagtagtaa aaaatttatc ttttcttttt tttattattt
     1081 ttgtttctta ttttctaaaa aattaaaaaa aattttggtg aattaagtat ttccttgata
     1141 acactggctt ttggcctcat tttaggttat catttgtaat tttatttcac agttgatatg
     1201 attgttatgt aaaataaaaa agtttaggat acatcgaatt tttggggaaa ttcttttggg
     1261 ggtatcctaa atcttttcta ttat
//
LOCUS       DAZKMZ010000226         1233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000226 DAZKMZ010000000
VERSION     DAZKMZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.234"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..340)
                     /locus_tag="ACG8M5_002936"
     CDS             complement(41..340)
                     /locus_tag="ACG8M5_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0360091.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(346..576)
                     /locus_tag="ACG8M5_002937"
     CDS             complement(346..576)
                     /locus_tag="ACG8M5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360092.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(835..948)
                     /locus_tag="ACG8M5_002938"
                     /pseudo
     CDS             complement(835..948)
                     /locus_tag="ACG8M5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          430 a          206 c          182 g          415 t
ORIGIN      
        1 gataaaatcc gttccgggct acctttttag gtagcctact ttaatctttg tatctttctg
       61 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgcttcga gcgtctagca
      121 catcagtcac gatatcaaac aaaaaatata cgtctttctt cattctagtt ttcgcagcag
      181 ggattttaaa gtagttctta ttagaatagt agagattaat taataagcta tcttcgattg
      241 ctaaaaagaa aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag
      301 gatcaaattt aaaccataat tgcgtctcat taaaaagcat aaccattact ccgatctatt
      361 tttaaactta gtctctacct ctaatatata tcgagttttt attttgcctt cagagaatac
      421 tgtttctttt tttgcagtta caggttgttt attttcggaa aaagctaata tagctaaaat
      481 tgaaacatcc atctggaatt tgtctttttc gttgctttgc tcataaaaat ctgcatattc
      541 atcactaata ttttttctaa taaattcttc catcataaag atcacctcga aaagagtata
      601 cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata ggtgaacaag
      661 tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa acaaaaagaa
      721 atcctattat gataacattt cttataataa caaacacaac agaagacgtg taatagttag
      781 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
      841 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
      901 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag
      961 agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
     1021 tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca
     1081 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
     1141 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
     1201 caactttcct tttcaaagta ccatataaaa aaa
//
LOCUS       DAZKMZ010000227         1220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000227 DAZKMZ010000000
VERSION     DAZKMZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.235"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..884
                     /locus_tag="ACG8M5_002939"
     CDS             294..884
                     /locus_tag="ACG8M5_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0360093.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            911..>1220
                     /locus_tag="ACG8M5_002940"
     CDS             911..>1220
                     /locus_tag="ACG8M5_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0360094.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     I"
BASE COUNT          435 a          134 c          246 g          405 t
ORIGIN      
        1 atttttggag atttatattc cagttatata ttaatgattg tgatacttgt actattgtaa
       61 aaattcgtta ggtttaaata actggaaata tcggaaaatg ggcattatac atcacggaca
      121 tcccttcttt tggaaaaaat gttacgattt ttataagtaa tattgtatcc ggtggaatca
      181 acgatgaaga taacatatcc atcgttgtag attcagtagc attgaatgat gctcttgttg
      241 caagaaatcc gaataaaatt gttaagtata aattgaatta ataggagata tgaatggaaa
      301 ttagcagtaa agaatattta aaagcggttc ttcgtatgga aaagaatcta tatatagaat
      361 ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc acatccggac tacaacgctt
      421 ggcggtatac tcgtcgaatg agaattacat cttattatta ttcaaagaga aagaaaaata
      481 tcatttatgc gattatgtat attgtgtctt gcagaagaat gaatagtttg ggaagaaaat
      541 gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt aagattttac catacacaag
      601 gaattgttat taatggtaac gccataatag gaaaaaattg ttacttatat gggaataact
      661 gtattggaaa tgatggaatc gatcctaagt gtcctgtaat tggaaataat gtacgtgtat
      721 gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa caatgtagtt gttgcggcag
      781 gagctattgt tatcaaagat tgtctagaga atggtgcaat tcttgcaggt gtaccggcaa
      841 aaataatagg gtatgttaat ttaacaaaag tatttttaga ataattttta gcgaaagaag
      901 gtgaataacg atgcgtattt caataaaaag atggtcagga gcagattgga cattgttcac
      961 gttggcattt tttgcaatct ccatagttct tgtatatact tttaacgccc caagagcaat
     1021 tttatatttg ggagatataa ttaatatctt tgtgttatta aatgcactta gctttagacg
     1081 cagaaaagga atatatgcta gcaaaaatga gatgctgata gttttgtttt tactagtttc
     1141 tatttgtagc tcagttatta atttagaaaa tataggattg attatctggg ggatacgaaa
     1201 taacactcgt atgttcatag
//
LOCUS       DAZKMZ010000228         1203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000228 DAZKMZ010000000
VERSION     DAZKMZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.236"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..>1203
                     /locus_tag="ACG8M5_002941"
     CDS             134..>1203
                     /locus_tag="ACG8M5_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0360095.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDK"
BASE COUNT          472 a          169 c          203 g          359 t
ORIGIN      
        1 aatattgcag gcttttttat tgatttgaaa taatcaagaa tattagaaac ttctacatta
       61 atcaagaact ttcttctgta tgaaaataga gataataatt gtacacttaa gctgtattca
      121 ggaggtgaag ataatgggca tcgaatttag cgaacggcaa atgaaaatac tatacttttt
      181 gattgaaaat gagcacacaa cttacaaaga attatcagaa caattatatg tatctacgaa
      241 aacaattagc aaagatattt taattttgaa agaaataatc caaaaaatgc cagatgtaca
      301 aatgttggtt caacctaaaa aaggcgtttc gcttattgga aaaaaagaaa cacttcgaaa
      361 tttattatcc agtcataaaa tcaatatcgc agactctccg gaggaccgtc taacttatgt
      421 gtttgctaaa ttattgaata ctaatgattc tctaaagatt caaaatcttg ctgacgaatt
      481 atacgtgagc aggtcgacgg tagaaagcac aatgaaagat gttagaaaaa gactaaacaa
      541 gcatgggatg tccttagttt cagatcgaaa tggcgtgcgc atcaaagctg atgaaaaaag
      601 taaaagaagg ttgatgtcag aagttgtgaa ctattattgg ggtggcttta cagcatctaa
      661 cagtaaaaca gatctcttag acttgaatat caatttcatt ggtgaagcga ataatttctt
      721 aaataaggac atattgaaaa aagtagccga attgcttaat cgatacatga ataagacaca
      781 attgaaattt acagattatg aatttcaaac tttagctatc caccttgcta ttgcgataga
      841 aaggattgaa acaagcaatt tcttatcacg tagtaataat tctgaagaag aaatctcaaa
      901 gaatacactt attttaatca aaatgattga aaaagcattt tcaattattg tgccaataga
      961 tgaacaagct tatgttgaca ttcacattac ttcgattgaa aaaaatgcta tgaatcacga
     1021 cttagatcaa acgaagcttt cctatttaga tatgggtgtt gagctacaag agttagtaag
     1081 aattaaatta aatgaatttt atcctgatga agaattgatc aataatctag tattacatct
     1141 caatgcagca gttaaaagac tcaaattggg agtaaatatc tataaccctt atacggacaa
     1201 aat
//
LOCUS       DAZKMZ010000229         1186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000229 DAZKMZ010000000
VERSION     DAZKMZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.237"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..257
                     /locus_tag="ACG8M5_002942"
     CDS             <1..257
                     /locus_tag="ACG8M5_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0360096.1"
                     /translation="NILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSP
                     SISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          425 a          129 c          245 g          387 t
ORIGIN      
        1 agaatatact tccaaaagat gaaagagtta gcgattttaa aaagataaat tcttttagta
       61 tgggattaag gatgaggaaa attaggaaag attattatct agaaagggag gagtttggaa
      121 agcttttttc tccttcaatt tcaaaatggt ccatagatag gtatgagaat ggtagagata
      181 tacccaatac taatcgaatt atccaatatg cttatatagg aaatctttct ttagaatttt
      241 tgatttacgg gatataaaaa tagtttacgt cgatacttat taagttatcg acgtttttta
      301 ttatagaaaa aatttataaa aggaggtttc ttgatatgat aagaaacaaa aagggtttgt
      361 tgaaagttgc tttactggca gcttttttct aagtaacttt ttgacaagct caatggcaac
      421 acaagcggaa gaagtaatac aagaactaac tgttatgaat aacagctaga taaaatgggc
      481 tatgtatgtt atccaacaat ttagtaatca ctagaagcct agcgaaagtt cgcttgataa
      541 gttatagatg agtattgatt tgtagtgttt ccaaatcaaa agggtacaga gccacatgaa
      601 atatgttgtc ctctgcagcg tcaggatggg aatccatacc atggagattc ggagatctgt
      661 gagggaatac ttacgtggtt aatatgtcag ttgtgttgga cgtagtaatg gtcatagtgg
      721 gtagattgac tgaattctta tatgagaaaa tgaaaccaac aagaagatag ggttatttat
      781 acagaattgg ggatcttttc tggaaaagta atcggataat gcgagattcg tacggtcttt
      841 tttaaatttc tctaaaaaaa acggcatcga atgggttggg tgattgataa gcaatgaatc
      901 atagactgat atagaataaa aaatgattct atttatcttt ttacatctat cgttttttca
      961 agtagccgca aattatatca gcattcaaca atttttgtat acaagttcta gtaaaaggat
     1021 ctgaagaaaa agttggttgt tgtgaagata aaaaaagaat caggttttac tgagtttttc
     1081 ttcaaaattt gtgttattag gttaggacaa agataagaag aagtatgaag atttaaaaaa
     1141 ggaatgagga aaagatagta atttccgatt attggagttt ttttat
//
LOCUS       DAZKMZ010000230         1155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000230 DAZKMZ010000000
VERSION     DAZKMZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.238"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..214)
                     /locus_tag="ACG8M5_002943"
     CDS             complement(<1..214)
                     /locus_tag="ACG8M5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360097.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHIN"
     gene            complement(231..>1155)
                     /locus_tag="ACG8M5_002944"
     CDS             complement(231..>1155)
                     /locus_tag="ACG8M5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0360098.1"
                     /translation="FKLKMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFF
                     TGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDI
                     RNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDAL
                     IQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVK
                     EIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSD
                     SSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          395 a          200 c          131 g          429 t
ORIGIN      
        1 cattaatatg actgatagat tctctagctt cttcacataa actttttact ttattaatta
       61 ctactttgtc atattcattt aacacactat tattaccatt acttagacgc tcagattcta
      121 cgaaacaact caaatcagaa atttcccaaa aaaatttttt gttaaaatcc aatttcctca
      181 actcctccaa actacgtaaa tgatatagtc tcataataaa ataaaacctc tcagctaatg
      241 ttgttgaaat tgtccactaa acctaattga tattgcttag gagatacacc tgcgtacttt
      301 ttgaatactc tcgaaaagtg tgaagaatct gaaaatccta aacctaaaga aacttcactg
      361 atagtatggt ttgatttcaa cattatttta gccgtagaaa tttttcgctt agagatataa
      421 ttataaatcg taatatgcat ttcattctta aagctttctc ttaatttagt ttcacttaca
      481 gaaaaataat tagctatttc ctttacagta attcttttat agatatttaa gccgatgaat
      541 tgaacgacag aagaaattaa gggagaaaga tgctctatct tagtttttcc gatttcctta
      601 gtataataaa caatcccaga atccctcact tttaagacct caggtaacga tacagctaat
      661 tcgttttctt gtatcaaagc atctctactt tgatacgctt ctattatatc cattcctaat
      721 gttatagacg tttgtgctaa cttttcaaaa acaatgatag aataattttt ttcagaccga
      781 atcgtatctc cactaacact tggaattatt ccattactca gattaaaaac catgttttct
      841 aaatttcgaa tatctccatt tttaacatat tccagtattt tattttcata aagaaacaga
      901 tatatttctg gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt
      961 ttacttagat tttctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
     1021 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
     1081 aaattttgca atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc
     1141 atttttagtt taaaa
//
LOCUS       DAZKMZ010000231         1113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000231 DAZKMZ010000000
VERSION     DAZKMZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.240"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8M5_002945"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8M5_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          391 a          202 c          222 g          298 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataaaat ttgtcaaaaa aactgttgcc att
//
LOCUS       DAZKMZ010000232         1113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000232 DAZKMZ010000000
VERSION     DAZKMZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.241"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1064
                     /locus_tag="ACG8M5_002946"
     CDS             <1..1064
                     /locus_tag="ACG8M5_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360099.1"
                     /translation="YLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPYVEEACNEL
                     IDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVCQG
                     LEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLNDLSLNELIL
                     QIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILE
                     EWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHL
                     YAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGA
                     NIHLYHIAQELHLLKKHIS"
BASE COUNT          407 a          156 c          211 g          339 t
ORIGIN      
        1 attatttgaa aaataaagaa atcattatcc cagaatttga ctcgcggaac atcttgctag
       61 ctttagtacg ttatgtcaat tgggaaggct atgaaaataa aaatccgtat gtagaagaag
      121 cttgtaacga gttgattgat tatgtagaga ctcatttttt taaaagaaga tattcggaag
      181 atgaaaagtc ctttatcata cgaggaatag agatcgcaat cggaagaaag gcatttcccc
      241 ttggactgaa agaagaagat aagaaaagcg cagaaacgaa gccgttattt caattagtct
      301 gtcaaggatt ggaagagcaa aaaggaattc tagacttaca agaagaagat gtctattata
      361 ttttttcttt gttcaacagt agaaattata ccaatgagaa catggagctg ttaagaaaag
      421 atttcgaagt tgtttataaa aattttgttc tcaatgacct atcactgaat gaattgattt
      481 tacagattat tagtatcgct gaaatcgacg atgcagaaaa ttttattttc aagcaatcct
      541 ttttacaatt tgtccgtacg ttgtgggcag atggacaagt atttttaccg gaaaggatct
      601 atcttctatc agaaaaagaa caacttctgt acggaaaaat tgtagaaata ttggaagagt
      661 ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa tttcattcga aaattcgtca
      721 aaagtctttc tttgatcaac agtgaacaag cggggagaca ggaaatagaa atttttgttg
      781 tttcagaaag ttcagtcaaa caattttttt atcgaaccca atttcatttg tatgcggaag
      841 aaggcatcgc tgaaataaat cctattttat ttcgttcatt agaagaattg cctgatgaat
      901 gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa aatggattag
      961 aaactgaaaa gacaaagatc tatccaatct cattaaaagg tgcaaacatc catttgtatc
     1021 atattgctca ggaacttcac ctattaaaaa aacatatttc ttgatgagac cttaattagg
     1081 agaccttcta caaagctttt taattgtctg tag
//
LOCUS       DAZKMZ010000233         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000233 DAZKMZ010000000
VERSION     DAZKMZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.242"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..179
                     /locus_tag="ACG8M5_002947"
                     /pseudo
     CDS             1..179
                     /locus_tag="ACG8M5_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
     gene            352..1065
                     /locus_tag="ACG8M5_002948"
     CDS             352..1065
                     /locus_tag="ACG8M5_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360100.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          373 a          206 c          183 g          339 t
ORIGIN      
        1 cgtaatttga ttactgcgaa tggaacagct cctatagaat tcacagactt gattcttcaa
       61 gcagtccatt ttgaaacaga agaaataaga gaaaaaacga ttcgtctata caaacttgga
      121 ttctatgatt actataaaaa gtacggagat cctttctcct aatcattctc ttcaattaaa
      181 tactccttat caatagtgtg acaagtaatt ttactgatga ttatttgtca cactttttct
      241 gacatttatc aaagaagata cactctattt atatccatag acaaggtttt acggattcaa
      301 aaattcagga ttatgctata ctggtattaa aaaaataatg aggtatttta tatgaaaaag
      361 ttaggtttta ttttattgtt ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa
      421 gaaaatagtg atccatctgc gaccacagac aaaagttcaa tcagacaaac cagttcagct
      481 gaaaaatcaa gtactcagaa atcagcttct actgaaacga atacatcttc aacaagcagt
      541 aaagtctcag agcccaatac ttcttctaca tctagtagta cagttgagac aaatatttct
      601 tcttcaacaa tagacacaac ctcctcacag tcttctcagt caatagatcc gttagcagga
      661 tattctgatc tacaaattga gtacgcacgt gtttggctag ctattagagg taatactttt
      721 agagatacat cgggtgattt cgaattacat gttattcatt atcctgcagg aacacctatc
      781 gccccttatg atgcagggag tgcagtctat ccgaaagatg tggttatgct cactgggaaa
      841 tacagttacc agggagtttt agtgtattca agtaaccatg acgggacaat cactagttat
      901 ccagttcctt cacattggca aatgcctgca gatcaaacat cttatccagc gttcattaga
      961 gaaacaactc aagaaatcat tgataatgca tctgtagtag ctattcctac aggaaatccc
     1021 aatgacgtga aacagttaat cgatgtgaca gttatagata attagacttt ctaatatgtt
     1081 atacaaaaag aagctgacag c
//
LOCUS       DAZKMZ010000234         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000234 DAZKMZ010000000
VERSION     DAZKMZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.244"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8M5_002949"
     rRNA            <1..>1073
                     /locus_tag="ACG8M5_002949"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKMZ010000235         1071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000235 DAZKMZ010000000
VERSION     DAZKMZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.245"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1071
                     /locus_tag="ACG8M5_002950"
     CDS             <1..>1071
                     /locus_tag="ACG8M5_002950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0360101.1"
                     /translation="LNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILA
                     TGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAI
                     RKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKI
                     VTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCA
                     TTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGL
                     NLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSG
                     LLSMFSLAIEEQITLDKLKMLDY"
BASE COUNT          417 a          126 c          185 g          343 t
ORIGIN      
        1 ctaaattctg agataaaaaa gttatttcta gagacagaag ttgacaaaat tgatattcaa
       61 aataaaaaga ttagttgtgt aaaaaaagat ggtacaagat tctctcaaga atatgacaaa
      121 gttatattag ctacaggttc aaagcaaatt atgctagata taccggggaa taatttagct
      181 aatatttttt cgataaagaa ttttccaaat gcaaaagaga tttttaaagc tttggatgat
      241 agtactatta aaaaagtagg aattattgga gcaggatata ttggagttga aattgctgaa
      301 gccataagaa aaagagggaa agaagtatat cttttcgatg ttgcagatag agtgttgtcg
      361 acttattacg accgtagttt ttcagataaa gttgaagaaa tactatcaaa aaatggaata
      421 catctttgtc tatcagaaag tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata
      481 gtcactacaa gtggtaccca atactctatg gatatggtcg tacaagctat aggatttcta
      541 tctaatagtc caattgggga aaaggaactc ttgagagatg ttagtggagc atatctaact
      601 aatgaatacc agcaaacaaa tataaaagat atatatgcta ttggggattg tgctacaact
      661 ttttttacgt ccattaatag aagtagtgtt gatttttcta tttctaatgc tctaagaaca
      721 gcttatattg caactcaaca tattaacaat caagatttta caccttctgg ttcccaattt
      781 acaaatggct tttctatatt taattacaat ttttacgcag caggtttgaa tttaaaaaca
      841 gcacgattat taagtgagtc tatagattat atagaaatag aagatttcat tagacctgct
      901 tttttgagaa aaaatagtcg tgtaaaatta agaattagct ttgaaaaaaa tacaaagaga
      961 atagtaggtg tacaactttg tagtcaagaa gatttatctg gactattatc aatgttttct
     1021 cttgctatag aagaacaaat tacattagac aaattgaaaa tgttagatta t
//
LOCUS       DAZKMZ010000236         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000236 DAZKMZ010000000
VERSION     DAZKMZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.246"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG8M5_002951"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG8M5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKMZ010000237          980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000237 DAZKMZ010000000
VERSION     DAZKMZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.247"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..250
                     /locus_tag="ACG8M5_002952"
     CDS             <1..250
                     /locus_tag="ACG8M5_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360102.1"
                     /translation="TIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHDVDRN
                     EILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(612..851)
                     /locus_tag="ACG8M5_002953"
     CDS             complement(612..851)
                     /locus_tag="ACG8M5_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360103.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          345 a          143 c          182 g          310 t
ORIGIN      
        1 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
       61 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
      121 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
      181 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      241 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      301 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      361 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      421 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      481 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      541 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      601 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      661 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      721 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      781 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      841 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      901 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      961 catatatgga gagggtgata
//
LOCUS       DAZKMZ010000238          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000238 DAZKMZ010000000
VERSION     DAZKMZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.248"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>961
                     /locus_tag="ACG8M5_002954"
     CDS             <1..>961
                     /locus_tag="ACG8M5_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFQ0360104.1"
                     /translation="ESDEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDV
                     IEETSRNFLLSKFRKFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVK
                     IPETHCLIVMHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSID
                     TTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQ
                     MDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNAL
                     KKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTL"
BASE COUNT          354 a          108 c          177 g          322 t
ORIGIN      
        1 gaaagtgacg aatttatagc attaagaaac tatgttgtta ataaaatatc accaattatt
       61 gattcgacag gtctatgttt attagatgaa ttcttggcgg atatcagtct atttataatg
      121 tttattgatg taattgaaga aacatcaaga aattttttgc tgtctaagtt cagaaaattt
      181 cgtatgaaaa atgataaaca aaaattattg gcaaatgaga tttggagtag tttagaagtc
      241 gaagaaagta aacaagaact tttgttgaaa tggatacttt ttcttgtaaa gaagttctat
      301 gtgaaaatac cagaaaccca ttgtctgata gttatgcacg gtaaaataac agcttctgcg
      361 atcgcaaggg aaacaaataa gttgctgaat acatatgttt atgaggcttt cgatatgcca
      421 attgaaggtg agactagtga cttaattaac ctaatcaaca atttttgtaa aagtatagat
      481 acgacgaatg gactcattct gctagttgat atgggatcat tggaacaaat gtatgaaaaa
      541 attgaatcca atgtaatagg tgatttggta attttaaaca atgtttctac agctttagcg
      601 attgaatgtg ggattcaagt ttgccaaaaa aagccaatta gtcattttta ccagatggat
      661 tttgattcgt ttcaagttaa agttcagtat tataaaggat tatcacaaaa gaaaaatgta
      721 atagtatctt gtctctctgg tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg
      781 tatctcgata atcaagtgga gattttgatt tttgatttta acgcattaaa gaaaatagca
      841 aaggaaaaag atactatgat ttttaagaat acaatttgtg ttctttcaac cactgaattt
      901 tacattcaag gaatcgattg tttaaattta gaaaatctaa ttaatgggaa tcaaacctta
      961 g
//
LOCUS       DAZKMZ010000239          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000239 DAZKMZ010000000
VERSION     DAZKMZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.249"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8M5_002955"
     CDS             <1..459
                     /locus_tag="ACG8M5_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360105.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8M5_002956"
     CDS             473..904
                     /locus_tag="ACG8M5_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360106.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKMZ010000240          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000240 DAZKMZ010000000
VERSION     DAZKMZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.250"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG8M5_002957"
     CDS             <1..878
                     /locus_tag="ACG8M5_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0360107.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          301 a          212 c          192 g          256 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 a
//
LOCUS       DAZKMZ010000241          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000241 DAZKMZ010000000
VERSION     DAZKMZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.251"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..447
                     /locus_tag="ACG8M5_002958"
     CDS             262..447
                     /locus_tag="ACG8M5_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0360108.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            460..708
                     /locus_tag="ACG8M5_002959"
     CDS             460..708
                     /locus_tag="ACG8M5_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360109.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            739..909
                     /locus_tag="ACG8M5_002960"
     CDS             739..909
                     /locus_tag="ACG8M5_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360110.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT          342 a          128 c          187 g          281 t
ORIGIN      
        1 aaagttatat ttttggttga caattagctt ttaagctagt atatttaatt cgtaagctaa
       61 attgttagct aaataagagc aacaaaaaac tctactaatt taaaacattc tctcggtcgc
      121 caaacttaga aatgttattt tagaggcttt ttataagtct tatttaacta tgtattcata
      181 atagcttaaa agctaatagg tgtcaacgat ttagctaatt ttttagctta caaattattt
      241 gtttagaaag gagctatttt tatgtctgag aatttagact taaaaattcg agcggagatg
      301 agaaaaagaa gaatgacttt caaagaacta gctgcgctta tcggtatttc aggagcttat
      361 ttatcagata ttctaaacgg caatcgtgat ggaaagaaag cacaacagca tatcgaaaca
      421 gtgaaaaaaa tattggacat ccgatagggg gggtaggcga tgacaaaact aaaaaaacaa
      481 gattttgtaa aaaaatataa ttattctcca tctacttatc aacgtcgaat gtcggaacta
      541 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
      601 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
      661 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
      721 tttgagaggt gaaggttaat gggtaaattc aacagagcat tagtattcag cgcaccgcta
      781 atcgtctatg ctttagggct ttggggaagt agacaagcgt tgataggaac gattgtgtac
      841 atggtttgga tttttatagg gctggatgaa gctgagtaca aaacaaaaaa gccagtcggg
      901 agggactgac taatgagtaa ggacgatatt gtatttct
//
LOCUS       DAZKMZ010000242          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000242 DAZKMZ010000000
VERSION     DAZKMZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.252"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8M5_002961"
     CDS             <1..355
                     /locus_tag="ACG8M5_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360111.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8M5_002962"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8M5_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKMZ010000243          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000243 DAZKMZ010000000
VERSION     DAZKMZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.253"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..875
                     /locus_tag="ACG8M5_002963"
     CDS             252..875
                     /locus_tag="ACG8M5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0360112.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aaga
//
LOCUS       DAZKMZ010000244          885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000244 DAZKMZ010000000
VERSION     DAZKMZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.254"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>885
                     /gene="bsh"
                     /locus_tag="ACG8M5_002964"
     CDS             <1..>885
                     /gene="bsh"
                     /locus_tag="ACG8M5_002964"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0360113.1"
                     /translation="CTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDL
                     DTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIP
                     WILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIY
                     DNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLS
                     SVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCC
                     NVDKGIYYYRTYEDSQIT"
BASE COUNT          298 a          120 c          163 g          304 t
ORIGIN      
        1 tgtgtacgtc tattacttat gtaacaagtg atcattattt tggaaggaat tttgattatg
       61 aaatatctta caatgaagta gtcactgtta ctccaagaaa ttataagttg aattttcgaa
      121 aggtaaatga tttggatact cattatgcaa tgattggtat tgccgctggt atagctgact
      181 accctcttta ttacgatgcg acaaatgaaa aaggattgag tatggctggg ctaaattttt
      241 ctgggtatgc tgattataaa gaaatacaag aagggaaaga caatgtatct ccttttgaat
      301 ttattccttg gattttagga caatgctcaa cagtaggaga agctaaaaaa ttgttaaaaa
      361 atatcaattt agcaaatata aattatagtg atgaacttcc tttatcccct ttacattggc
      421 tattagctga taaagaaaaa tcaattgtca ttgaaagtat gaaagatgga cttcatatat
      481 atgataaccc tgtgggcgtt cttaccaata atccttcatt tgactatcaa ttatttaatt
      541 taaacaatta tcgtgtctta tcgagtgaaa ctcctaaaaa taatttttca aatcaaataa
      601 gtttgaatgc ctatagccgc ggtatgggag ggataggctt gcctggagat ttatcctcag
      661 tatctcgttt tgttaaagcg acttttacga agctgaattc tgtatctgga gattcagagt
      721 cagaaagtat tagtcaattt ttccatatct taggttcagt agaacaacaa aaaggtttgt
      781 gtgatgttgg tgatggaaaa tatgaatata caatttattc ttcttgttgc aatgttgaca
      841 aaggaatcta ttattatcga acatatgaag acagtcaaat tactg
//
LOCUS       DAZKMZ010000245          872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000245 DAZKMZ010000000
VERSION     DAZKMZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.255"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACG8M5_002965"
     CDS             <1..>872
                     /locus_tag="ACG8M5_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0360114.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DAZKMZ010000246          855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000246 DAZKMZ010000000
VERSION     DAZKMZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.256"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..810
                     /locus_tag="ACG8M5_002966"
     CDS             <1..810
                     /locus_tag="ACG8M5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0360115.1"
                     /translation="QEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRT
                     IDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYIN
                     ECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNN
                     YILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNM
                     DKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          383 a           58 c          121 g          293 t
ORIGIN      
        1 caagaaataa aaattgttac tgataaaatg atgaaacttg ataaaaaaat gagtttaaaa
       61 gttagctcta atgatgaagt tggagaatta aaacaacaaa ttaatgattt gtattctact
      121 ttattaagaa caattgacga tttggaattt aaaaataaag aaattttaaa attagaaaag
      181 ttaaaatatg acttttttaa aggtgcatcc catgaactta aaacccctct tgctagtctt
      241 aaaataatac tggaaaacat gaaatacaat attggaaaat ataaagaaag agatatttat
      301 attaatgaat gtattgaaat cgttgatagt ttaacaaaaa atatttctca aatattatca
      361 gttcattcta tagaaaattt gaataatgat gaagaatatt tgaaaataaa tgatacatta
      421 gaagacatat taaaaaagta tgaaatatta gcaaatcaaa agaaaataac tgtcaataat
      481 tatatattag acgagaatgt ttatatagga aaaacagctt taaagattat tctatctaat
      541 ttgattagta atgcggtaaa atatactgat gtaaatgggg taattaatat tgggatagtt
      601 aatgattggt tatatataga gaattcatac ggtaacaata aaatttcgaa tatggataaa
      661 atatttgatg ttaaatttga tttaaataag gaaaatagta atggattagg tttatatatt
      721 gtaagtaata ttctgaataa ttacaatata gaatataaag tattgcagga tgaagaattt
      781 tttatcttta aaataaaaat attttcttaa ttaagatata attttgcaat agttattgaa
      841 aaatagagac ttatt
//
LOCUS       DAZKMZ010000247          824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000247 DAZKMZ010000000
VERSION     DAZKMZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.257"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..>824
                     /locus_tag="ACG8M5_002967"
     CDS             76..>824
                     /locus_tag="ACG8M5_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0360116.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          298 a          148 c          149 g          229 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      361 cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      421 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      481 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      541 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      601 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      661 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      721 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      781 aaccaacatg atattgatat atattttgcg gaccctggaa cacc
//
LOCUS       DAZKMZ010000248          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000248 DAZKMZ010000000
VERSION     DAZKMZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.258"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8M5_002968"
     CDS             <1..672
                     /locus_tag="ACG8M5_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0360117.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKMZ010000249          777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000249 DAZKMZ010000000
VERSION     DAZKMZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.259"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>777
                     /locus_tag="ACG8M5_002969"
     CDS             <1..>777
                     /locus_tag="ACG8M5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0360118.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVA"
BASE COUNT          261 a          135 c          152 g          229 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgca
//
LOCUS       DAZKMZ010000250          759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000250 DAZKMZ010000000
VERSION     DAZKMZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.261"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..428
                     /locus_tag="ACG8M5_002970"
     CDS             21..428
                     /locus_tag="ACG8M5_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360119.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            442..>759
                     /locus_tag="ACG8M5_002971"
     CDS             442..>759
                     /locus_tag="ACG8M5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360120.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSD"
BASE COUNT          284 a          101 c          136 g          238 t
ORIGIN      
        1 gtaaaaatta ggagaaatca atgtcttata gagtacaatt aataatttca gaagatgtag
       61 aaagtcagca atttggcact aatgttatta ataaagtaat taatccagct ctttctatta
      121 atgcgccttt aattcctact gctttatcat tttctgtaac agctgtggtg tctgagatag
      181 aggatacaga aaagataaaa atagtagaaa ttgaagtttt aaacaagaat gaaaaacaaa
      241 ttttttctac gggtgaagta tcggtaagct tgcctcctca agttaacgat ataaacttta
      301 atataaacgc cagaaatgtc ttggttgaag aagcaggaga gcattatgct gttgttaaat
      361 ttaatggaac tgagattgga aggcaaatct ttgatatcaa ggtcaacaaa ccagtggaaa
      421 aaaattaagg agacgatacg gatgcttgat atagtaccat caaatacaca tcggagtggt
      481 agcttaataa attttgctgc gatttttgct tgcgcatcgg taattctagc atctccagtt
      541 gttgtggatg caagttctac tccgaaaact aaaaatgata atcaacttgt aattactaca
      601 aaaaatgaga ttaacacaat ttcaagtaat gatggcaaaa tttttgatgt gataaatacg
      661 gttttaaaaa gaaaagctga aatcaatata aaatatgatg aagatttaaa cttgtatttc
      721 tttgttatca aaacaacatc tgaattgttt tcctcagat
//
LOCUS       DAZKMZ010000251          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000251 DAZKMZ010000000
VERSION     DAZKMZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.262"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..475
                     /locus_tag="ACG8M5_002972"
     CDS             107..475
                     /locus_tag="ACG8M5_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0360121.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          269 a          125 c          113 g          247 t
ORIGIN      
        1 tataaatata gcggttacaa cattacttgt gttttgttcg ttttttatac ataactattg
       61 tttttctggt aaaataaatc aaaccactta atggaggcgt tttagaatgg aaaaatttca
      121 gccgatcatc aatcaaatca aagaaaaaga acatcaacaa aagatcaaag acctttttgc
      181 taagataaca gaaacgtatc cccagctaga cacaactgta aaatggaacc aacctatgtt
      241 tactgaccat ggtaccttta ttattgcatt tagttttgct aaaaatcatt tttctatcgc
      301 accggaaaaa gcagcgattc gtgcccttga aaaaaatatc caagaagcag gctacgtata
      361 tacggataac gtcatcaaag ttccttggaa aagtgtaatc aactgggaat tgattgaaca
      421 attgatttca tttaatattg aagataaaaa aggtcatgat aaattttggc gataaagctt
      481 tatacgaata aaccttttac tggcgaggaa actattcatc accttataca ggttttgttc
      541 aatcctatcc tttttatcat ccctggttaa acacttaatt gacaaagtat ctgctatttt
      601 tataaggagt ctttcttgtg ctgaaaaaaa tcactgattg aatggctttg aattttctac
      661 ttagtaaaat tattttttca gcaattttcc tgcaaaactt ttgaataagg aaaaaatacg
      721 agaaaatatc agtgatcttt cataatactg ccca
//
LOCUS       DAZKMZ010000252          732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000252 DAZKMZ010000000
VERSION     DAZKMZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.263"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8M5_002973"
     CDS             132..344
                     /locus_tag="ACG8M5_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360122.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>732
                     /locus_tag="ACG8M5_002974"
     CDS             506..>732
                     /locus_tag="ACG8M5_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0360123.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSK"
BASE COUNT          265 a          105 c          126 g          236 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aa
//
LOCUS       DAZKMZ010000253          732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000253 DAZKMZ010000000
VERSION     DAZKMZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.264"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          267 a          124 c          108 g          233 t
ORIGIN      
        1 ttgaagcata ctagagctaa ctaaaatagc actagaaagc aatgttaaac ccgtccaaca
       61 aacttgattt aacattgaga ccagcttatt cagctaaata tagttgaata agcttctttt
      121 gttttaaaaa aacaaaaggt gtactacgaa taacgcaagt aaaagaaata ctattacaac
      181 cagtaggcta caagtaagta ttactaaaca ttgcgtagtt agaacctttg ctctattgtc
      241 taaagccaca aaattagaga tcagctttat gatgctaaga gcgagctgca tccatgcgga
      301 cataggttct tttgacacta ttaattctcc tttttaggat aagggaaatt gctagtagtt
      361 ttttcattag caccatcccc cttcatttga ttgagtctag tgctgctgtg atagtttgct
      421 gtttaatatg cttacattct tattataaca taaatgaaac aaacacatgg ttagaggtcg
      481 tatcaatcaa aatagtgtct cacaaacgat ttattactta ctcctgctga ctaacggact
      541 gttcttcagt aactgtaact atatgtttgt taaaaccacc attacctgta gataaaaacc
      601 aatatattta ctatactcac tatattaaat atatttagta tagtaaaaac tagaaacaat
      661 aaaatttgtg aatgttgtat acccccaaac aatacacata ttttgaagga ttaggattaa
      721 taaagctaaa aa
//
LOCUS       DAZKMZ010000254          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000254 DAZKMZ010000000
VERSION     DAZKMZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.265"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..509
                     /locus_tag="ACG8M5_002975"
     CDS             24..509
                     /locus_tag="ACG8M5_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360124.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          223 a          135 c          123 g          250 t
ORIGIN      
        1 aatctatttt aggaggagct atcatgcata aagggattta cgcaggcttg ttgggaactg
       61 taatactttt ttctgcctgt ggatataaaa caaagaattc tgttaaagaa tcttcagcag
      121 ccatttcaac aactgaggtt ggttcaacta aaagtacttc tactacttcc attactaata
      181 cttctactgg taatgtagat tcgcaacata gccaatcaag tattcagtcg tttagcattt
      241 cttcaacatc tgaaatgcaa gaaagcactg taccttctct tccattagaa aactctgaca
      301 aagcacttct atttcttatt gcccaccgtt ccgaattaca aagtgacgat attgttatta
      361 gcttttatca aaaaattgat cgagattatt tgttcacagc ttccagcaaa caaatccgtt
      421 cccaaggagg atctggaagc gttggttttt atcgagtgtc acccgaagga tcgattacga
      481 tgacagatgc aaatggaaca ccattttgat tagatgatat tatacggatt tacagtacta
      541 gcaagtcatt attggtatca ttttttctga gaaagttata cggagtataa ataagcattt
      601 gcattaacct aataattcgt tacagctatt tctcttatga atatactcac ttctctactt
      661 gctctcttga taaatttttg gtatctactt cattttcggt caactgagtt aatgaaattt
      721 attgtcgggc g
//
LOCUS       DAZKMZ010000255          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000255 DAZKMZ010000000
VERSION     DAZKMZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.266"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8M5_002976"
     CDS             <1..>725
                     /locus_tag="ACG8M5_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0360125.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKMZ010000256          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000256 DAZKMZ010000000
VERSION     DAZKMZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.267"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG8M5_002977"
     CDS             <1..600
                     /locus_tag="ACG8M5_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0360126.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKMZ010000257          702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000257 DAZKMZ010000000
VERSION     DAZKMZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.268"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..425
                     /locus_tag="ACG8M5_002978"
     CDS             <1..425
                     /locus_tag="ACG8M5_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0360127.1"
                     /translation="KKERVIVPKIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYE
                     FRISLINNQLTFGKTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNV
                     ALSTRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
     gene            448..>702
                     /locus_tag="ACG8M5_002979"
     CDS             448..>702
                     /locus_tag="ACG8M5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0360128.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDL"
BASE COUNT          260 a           79 c          141 g          222 t
ORIGIN      
        1 gaaaaaaaga aagagtaatt gttcctaaaa tcgaagatta ttttctattt gaaacaactt
       61 tgactattac atcgttaaca aaggagtttg ctttaaaatt atttgtagat agtgatactc
      121 aagaaggata cgaatttcgt atttcattga ttaataatca gcttactttc ggaaaaactc
      181 cgaactatcc ttggcctcaa atgtttgata aggggctgca acgaccacta tttttggaaa
      241 taggcaaaga atatgaacta aagttaattg ttgatgatac gatagctgtt ttatatattg
      301 ataatgttgc tttaagtact agaatgtatg ataaagtcgg aacagcatta agtatgactg
      361 ttacggaggg agcaatcaag gtagataaac tagactattc aataaattat gaatgtggat
      421 tttaaaacaa ttgaagggat gatgtaagtg aaattaaatg gtttacaaca tattggaatt
      481 ccaacaaaag attttggagc ttcaaaaatc ttttatgaat ccttagggtt tagtctgatt
      541 aatgaagaaa tgaatgtggg aagtaaagtc ggctttttcg agttgaatgg aacgataata
      601 gaaatatggg aagataaaac tactgagtgt agaggagcca ttgatcatct agcattagat
      661 acggatgaca tagatgaggt attcaattca attcaagatt ta
//
LOCUS       DAZKMZ010000258          698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000258 DAZKMZ010000000
VERSION     DAZKMZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.269"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..629)
                     /locus_tag="ACG8M5_002980"
     CDS             complement(<1..629)
                     /locus_tag="ACG8M5_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360129.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLIS"
BASE COUNT          247 a          102 c          100 g          249 t
ORIGIN      
        1 gaaatcagtt gaatgggtat gtgattgatc aataataata aataactgaa aaaaagaaaa
       61 aatttatctt gaagcaactc tttgcgtttt gacgttagat catttatata ctctatcaaa
      121 tagcaataaa atgctggata gttttctcta aaaaaagaaa catcaagtgg aaattcatca
      181 gctttattaa tagtgtaatg caaaagttta aaatgtattt tggttaactc tcttcggatc
      241 aattggctcg tctcttctcg caacagatta aactgatttt caaaactttc aatgaatcgt
      301 tcattcaacc attcgattgt ttcatcttca ttttttctca agtatttttg atccaattta
      361 tcatgaagat aaagccaaga gagtgtctta ttgataaaaa taacggattt ttctcctaat
      421 actcctttta agtggtaaaa tcctatatcc gaaaaaaata gcgactgatt gctagcaata
      481 tctaaagaat cagcttgttc agccaacagg ttgattgcta aaaagtggaa taatttcttt
      541 ttttcataag cagacagagg ataatactgt tttaaatctt ttaacatttt ttctgttttt
      601 ataattatag gtagcgtata caattccaca aaacttatct ctgttgagtt aaaaagatta
      661 tataaaaaaa gccgaatgtt tttaggattc cctgtcaa
//
LOCUS       DAZKMZ010000259          691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000259 DAZKMZ010000000
VERSION     DAZKMZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.271"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG8M5_002981"
     CDS             complement(<1..461)
                     /locus_tag="ACG8M5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0360130.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          220 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga c
//
LOCUS       DAZKMZ010000260          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000260 DAZKMZ010000000
VERSION     DAZKMZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.272"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8M5_002982"
     CDS             154..417
                     /locus_tag="ACG8M5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0360131.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACG8M5_002983"
     CDS             417..>670
                     /locus_tag="ACG8M5_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0360132.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAZKMZ010000261          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000261 DAZKMZ010000000
VERSION     DAZKMZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.275"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..557)
                     /locus_tag="ACG8M5_002984"
     CDS             complement(<1..557)
                     /locus_tag="ACG8M5_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0360133.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFI"
BASE COUNT          272 a           92 c           68 g          178 t
ORIGIN      
        1 ctaataaata aaaatagaac aacaaacatt tgaatgatta aaaatattaa gacaccaaac
       61 acaaaatgat caaatacttg atattgatga gcaacataag aaacaaataa accaattgta
      121 agtaaataaa tcatgaatat agcagtaagt ttagaactgg actgttcaac aatcctattt
      181 atagctaatt ttctatataa ccaaataaac atcagcaaaa acaaagaatc aagcattatc
      241 tggaatacta cataaccata cccttttatc ccttcaaatg aaaacatata cgtcataacc
      301 atggaagtaa taatagatct gaaatcatca ataagcatac ataatagtaa tttatttaaa
      361 agcactatat tagttttctt cttttccttt aatataaaat aacttccgat tataggtata
      421 gctgcggaaa catcagtaaa taattcaaca aaaaaagtga gtggtatcaa taaacataca
      481 aaaagatatt ttcttttgtt aagatcagta ttagtcatat aaaataagag aaaaaagaaa
      541 aagtttccca cgaacattct taatagataa gcttttgtat cgatagtaat caagagatcc
      601 cctcctgatt
//
LOCUS       DAZKMZ010000262          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000262 DAZKMZ010000000
VERSION     DAZKMZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.276"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG8M5_002985"
     CDS             <1..580
                     /locus_tag="ACG8M5_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0360134.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKMZ010000263          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000263 DAZKMZ010000000
VERSION     DAZKMZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.277"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8M5_002986"
     CDS             complement(38..>583)
                     /locus_tag="ACG8M5_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0360135.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKMZ010000264          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000264 DAZKMZ010000000
VERSION     DAZKMZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.278"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..>561)
                     /locus_tag="ACG8M5_002987"
     CDS             complement(489..>561)
                     /locus_tag="ACG8M5_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0360136.1"
                     /translation="NGIRYFNFYGPNHEIIEISQMKK"
BASE COUNT          199 a           91 c           85 g          186 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt t
//
LOCUS       DAZKMZ010000265          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000265 DAZKMZ010000000
VERSION     DAZKMZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.279"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..524
                     /gene="spxA"
                     /locus_tag="ACG8M5_002988"
     CDS             126..524
                     /gene="spxA"
                     /locus_tag="ACG8M5_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0360137.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          158 t
ORIGIN      
        1 taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga
       61 tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt
      121 gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt
      181 ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca
      241 gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga
      301 cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat
      361 tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata
      421 aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac
      481 gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac
      541 ttggaaaaga c
//
LOCUS       DAZKMZ010000266          529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000266 DAZKMZ010000000
VERSION     DAZKMZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.280"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..199
                     /locus_tag="ACG8M5_002989"
     CDS             77..199
                     /locus_tag="ACG8M5_002989"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360138.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            172..303
                     /locus_tag="ACG8M5_002990"
     CDS             172..303
                     /locus_tag="ACG8M5_002990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360139.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          198 a           81 c           93 g          157 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttaaatcta tattttgata tacaaattac
       61 agcttcagat actaagttgc ataaaccttt tccggacccc atacaaaagg caattgatga
      121 attgggcata aaaaataaga aacgatttat attggtgatt cattatatga catgcaaagt
      181 gcgaaagctt gtgggatagc atttggttta gctaaatggg gagccaaaaa tatagaaaat
      241 tttcaaagtg tagatataat tattgattct cctcaagaaa tacttgattt gttagaaaaa
      301 tagacaaaga gcgtgagata aggatagggt ctcacgctct ttgtttttcg ccatttaaag
      361 attgaaattt ttgccatcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg
      421 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
      481 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaat
//
LOCUS       DAZKMZ010000267          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000267 DAZKMZ010000000
VERSION     DAZKMZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.281"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8M5_002991"
     CDS             <1..354
                     /locus_tag="ACG8M5_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0360140.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKMZ010000268          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000268 DAZKMZ010000000
VERSION     DAZKMZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.282"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>521
                     /locus_tag="ACG8M5_002992"
     CDS             25..>521
                     /locus_tag="ACG8M5_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0360141.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          190 a           95 c           86 g          150 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt a
//
LOCUS       DAZKMZ010000269          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000269 DAZKMZ010000000
VERSION     DAZKMZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.283"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8M5_002993"
     CDS             complement(<1..132)
                     /locus_tag="ACG8M5_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0360142.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKMZ010000270          481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000270 DAZKMZ010000000
VERSION     DAZKMZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.285"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..312)
                     /locus_tag="ACG8M5_002994"
     CDS             complement(10..312)
                     /locus_tag="ACG8M5_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0360143.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          169 a           82 c           65 g          165 t
ORIGIN      
        1 tagcctactt taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata
       61 acgccgatga tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac
      121 atctttcttc attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata
      181 gagattgatt aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt
      241 ataaaaatct ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc
      301 ttccatcaac ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag
      361 taggaatatc attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg
      421 ctattttgaa tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa
      481 a
//
LOCUS       DAZKMZ010000271          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000271 DAZKMZ010000000
VERSION     DAZKMZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.286"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8M5_002995"
     CDS             <1..>468
                     /locus_tag="ACG8M5_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0360144.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKMZ010000272          467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000272 DAZKMZ010000000
VERSION     DAZKMZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.287"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>467
                     /locus_tag="ACG8M5_002996"
     CDS             <1..>467
                     /locus_tag="ACG8M5_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0360145.1"
                     /translation="WKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLR
                     KKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLN
                     NLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          159 a           91 c           95 g          122 t
ORIGIN      
        1 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
       61 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
      121 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
      181 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      241 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      301 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      361 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
      421 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctact
//
LOCUS       DAZKMZ010000273          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000273 DAZKMZ010000000
VERSION     DAZKMZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.292"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8M5_002997"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8M5_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKMZ010000274          436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000274 DAZKMZ010000000
VERSION     DAZKMZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.294"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..86)
                     /locus_tag="ACG8M5_002998"
     tRNA            complement(13..86)
                     /locus_tag="ACG8M5_002998"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
     gene            complement(253..>436)
                     /locus_tag="ACG8M5_002999"
     CDS             complement(253..>436)
                     /locus_tag="ACG8M5_002999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360146.1"
                     /translation="IKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQK
                     ILYFSTFLSLTKIKKR"
BASE COUNT          144 a           71 c           86 g          135 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatg
//
LOCUS       DAZKMZ010000275          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000275 DAZKMZ010000000
VERSION     DAZKMZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.295"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACG8M5_003000"
     CDS             74..403
                     /locus_tag="ACG8M5_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0360147.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAZKMZ010000276          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000276 DAZKMZ010000000
VERSION     DAZKMZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.296"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8M5_003001"
     rRNA            <1..>411
                     /locus_tag="ACG8M5_003001"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKMZ010000277          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000277 DAZKMZ010000000
VERSION     DAZKMZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.297"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M5_003002"
     CDS             <1..>386
                     /locus_tag="ACG8M5_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0360148.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMZ010000278          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000278 DAZKMZ010000000
VERSION     DAZKMZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.298"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M5_003003"
     CDS             <1..>386
                     /locus_tag="ACG8M5_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0360149.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKMZ010000279          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000279 DAZKMZ010000000
VERSION     DAZKMZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.299"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACG8M5_003004"
     CDS             98..>369
                     /locus_tag="ACG8M5_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360150.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAZKMZ010000280          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000280 DAZKMZ010000000
VERSION     DAZKMZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.300"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8M5_003005"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8M5_003005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKMZ010000281          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000281 DAZKMZ010000000
VERSION     DAZKMZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.302"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8M5_003006"
     CDS             <1..>344
                     /locus_tag="ACG8M5_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0360151.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKMZ010000282          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000282 DAZKMZ010000000
VERSION     DAZKMZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.303"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..>343
                     /locus_tag="ACG8M5_003007"
     CDS             191..>343
                     /locus_tag="ACG8M5_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0360152.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQLPP"
BASE COUNT          122 a           61 c           50 g          110 t
ORIGIN      
        1 tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca ttatcaacta
       61 aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag
      121 ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct
      181 ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca
      241 gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca
      301 agaagaaatt caaaaacatg aacaacttga tcaactccca cca
//
LOCUS       DAZKMZ010000283          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000283 DAZKMZ010000000
VERSION     DAZKMZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.304"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8M5_003008"
     CDS             <1..>341
                     /locus_tag="ACG8M5_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0360153.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKMZ010000284          334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000284 DAZKMZ010000000
VERSION     DAZKMZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.306"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..>334
                     /locus_tag="ACG8M5_003009"
     CDS             162..>334
                     /locus_tag="ACG8M5_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0360154.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          144 a           49 c           47 g           94 t
ORIGIN      
        1 cctataattt tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa
       61 atgaaatcaa gaaatatact gtattactca attcattgaa aatatttatc ataattttta
      121 atagaaaaca ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac
      181 ttgcatcagc agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg
      241 caagcgaaaa ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta
      301 attccgatgt aacagcagag agtagtgaac tttt
//
LOCUS       DAZKMZ010000285          331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000285 DAZKMZ010000000
VERSION     DAZKMZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.307"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          131 a           47 c           65 g           88 t
ORIGIN      
        1 atatatagaa ggctttacag tatatatggg agcttactaa aaaagaatgg agtaagagtt
       61 aacacaatgg gccattagct gaagttttaa gcacttcaag gtatgaaaca gaatggcctg
      121 taatactaca tgattattta gactgctcaa actgctatca gtctggtgga atgaataaag
      181 tattatacta aaagcagatc caactacaac gtaacaggaa accagttgta tcaggaaaag
      241 tgatagtaca tggagaagaa aaggccccct ttgtatgagt cagtacatat cttatttata
      301 gataaaacgg tattcaatcg aaataaaaaa t
//
LOCUS       DAZKMZ010000286          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000286 DAZKMZ010000000
VERSION     DAZKMZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.308"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG8M5_003010"
     CDS             <1..288
                     /locus_tag="ACG8M5_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360155.1"
                     /translation="AYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESA
                     SQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          120 a           53 c           68 g           84 t
ORIGIN      
        1 gcctataatc aatttatcaa gaaaaattta agcttgtaca ttaatctgaa cacggaagtg
       61 atagaggcag gaaagcctga gttcccggat gttataatca cgaataggaa taatctatat
      121 gaagaaagtg catctcaagt agtggtttgg ctcgatccgc caagagcagt agactggggg
      181 aatcttacaa agattttgtt gactatccaa gaagagaaat acaaagacag caaaattgac
      241 aagcaaatag acgattttcc tgaagaaatc atcgacagga tcgaatagtg aacttctttt
      301 agaaagtccg actgcttttt ctaaa
//
LOCUS       DAZKMZ010000287          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000287 DAZKMZ010000000
VERSION     DAZKMZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.denovo.309"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG8M5_003011"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG8M5_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZKMZ010000288        11562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.080, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000288 DAZKMZ010000000
VERSION     DAZKMZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.080"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..1365)
                     /locus_tag="ACG8M5_003012"
     CDS             complement(25..1365)
                     /locus_tag="ACG8M5_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0360156.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1634..2530
                     /locus_tag="ACG8M5_003013"
     CDS             1634..2530
                     /locus_tag="ACG8M5_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0360157.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2680..3621)
                     /locus_tag="ACG8M5_003014"
     CDS             complement(2680..3621)
                     /locus_tag="ACG8M5_003014"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0360158.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3747..4868
                     /locus_tag="ACG8M5_003015"
     CDS             3747..4868
                     /locus_tag="ACG8M5_003015"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0360159.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5126..6628
                     /gene="eat(A)"
                     /locus_tag="ACG8M5_003016"
     CDS             5126..6628
                     /gene="eat(A)"
                     /locus_tag="ACG8M5_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0360160.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6695..7222
                     /locus_tag="ACG8M5_003017"
     CDS             6695..7222
                     /locus_tag="ACG8M5_003017"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0360161.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            7433..8494
                     /locus_tag="ACG8M5_003018"
     CDS             7433..8494
                     /locus_tag="ACG8M5_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0360162.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            8497..9393
                     /gene="murQ"
                     /locus_tag="ACG8M5_003019"
     CDS             8497..9393
                     /gene="murQ"
                     /locus_tag="ACG8M5_003019"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0360163.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            9407..11389
                     /locus_tag="ACG8M5_003020"
     CDS             9407..11389
                     /locus_tag="ACG8M5_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0360164.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11454..>11562)
                     /locus_tag="ACG8M5_003021"
     CDS             complement(11454..>11562)
                     /locus_tag="ACG8M5_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0360165.1"
                     /translation="QYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3863 a         2087 c         2451 g         3161 t
ORIGIN      
        1 ataaaataaa attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt
       61 ttgataactg aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga
      121 gttcgtgaac aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat
      181 tcctttatcc cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat
      241 gatccagatc aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc
      301 accgacaaaa taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc
      361 acgttgcggt acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc
      421 cttcaaacgg aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa
      481 caaagcaagg acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc
      541 aaataccatc acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat
      601 caaaagcaaa aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat
      661 tgctttagga atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc
      721 agaaccgccg taagaataga taactacaag caaagaactg atgattccct tcataccatg
      781 aggagtaaac ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa
      841 catttgctga ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt
      901 agcaaaagcc agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat
      961 cgtagttaaa acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa
     1021 ctgcaaaaag gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca
     1081 gtagacccaa tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga
     1141 taatccgtga tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat
     1201 atataaaatc agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat
     1261 cgcttcgcca cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt
     1321 gatatgtctg gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct
     1381 tttaaattca caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat
     1441 gataacaaat tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga
     1501 agaatgattt atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa
     1561 acgttttact ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag
     1621 tgaggcggaa aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta
     1681 ttttttgcct tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga
     1741 attcattaaa tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta
     1801 tggatttgat atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt
     1861 atttttttgg aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga
     1921 accggcaatc gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa
     1981 acataatagt tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga
     2041 tctatttttt ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga
     2101 atttataaaa agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca
     2161 acctggatat cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct
     2221 ggaactgttg cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt
     2281 tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga
     2341 aaatattttt ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc
     2401 atttaagcca atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg
     2461 atttatcact aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga
     2521 aaacaaataa aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc
     2581 ttatggcatc atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga
     2641 accagctgct gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc
     2701 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     2761 ttgtcctttt cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     2821 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     2881 agcaatatgt tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga
     2941 agcaaattca gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc
     3001 agcaatatta caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt
     3061 tcgccaaatg gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa
     3121 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3181 ttctccatca cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3241 gatcccaaat aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa
     3301 tccatttagt aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa
     3361 catatcatct aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct
     3421 ttcgatgatt tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa
     3481 gtcagcaatc aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa
     3541 gaggacctca tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc
     3601 cccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     3661 tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt
     3721 acttaagaag aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag
     3781 gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag
     3841 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     3901 acgcgctcag gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa
     3961 aaatcagcta tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac
     4021 aagcattggg gttagatcta cttgataaac caacggatca aactatccga cttgcttcca
     4081 gttatgcctt aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg
     4141 tcactttagg aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg
     4201 cttctttaga agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg
     4261 atcgaagaaa cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc
     4321 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata
     4381 cgacgctagc tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct
     4441 gggaggtgga tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc
     4501 tttttttgtt gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat
     4561 tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc
     4621 aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac
     4681 caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc
     4741 caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc
     4801 aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc
     4861 cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt
     4921 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     4981 tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg
     5041 ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg
     5101 gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5161 ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg
     5221 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca
     5281 aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca
     5341 acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga
     5401 gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag
     5461 agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt
     5521 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggtag
     5581 aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga
     5641 ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga
     5701 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     5761 aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga
     5821 aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat
     5881 cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat
     5941 gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact
     6001 attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa
     6061 gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat
     6121 tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa
     6181 gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata
     6241 ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac
     6301 gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg
     6361 aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca
     6421 acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga
     6481 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa
     6541 cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac
     6601 tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt
     6661 taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     6721 aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa
     6781 aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac
     6841 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg
     6901 tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga
     6961 tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt
     7021 cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg
     7081 catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat
     7141 caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac
     7201 agaattggtt tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc
     7261 actgcttttg cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact
     7321 caacctttag aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag
     7381 atatatactc tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg
     7441 cttttcaata tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat
     7501 ggcatccaat ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc
     7561 ggcatatcaa aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt
     7621 gatggtcgat atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac
     7681 gcctttgaaa gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca
     7741 gattgccgac tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa
     7801 agatatcgat gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa
     7861 ttattatcca cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg
     7921 gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg
     7981 cccactatat caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg
     8041 cgctctagat ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc
     8101 agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac
     8161 ggaacctgta gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga
     8221 cgatgcaaga gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc
     8281 agcaagaaat acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt
     8341 gcgctatatg ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa
     8401 tgtagtagct aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat
     8461 gaactatcaa tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac
     8521 ctaacaacgg aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa
     8581 gccgttcaac taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg
     8641 acaagtatcg aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg
     8701 atttatatgg gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta
     8761 ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca
     8821 atgactgtag ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc
     8881 aagctgaatt tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca
     8941 tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct
     9001 tgcaataaac aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt
     9061 ccagaatttt tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg
     9121 aatatgattt ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc
     9181 gatgtcaaac caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg
     9241 acagaagctg attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag
     9301 ctggctatcg tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct
     9361 gaagcaaacg gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga
     9421 actgactttg gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt
     9481 agaaagcatt gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt
     9541 aaatgtctct gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct
     9601 tcaagtcatc atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc
     9661 aggcgtgaaa ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc
     9721 atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa
     9781 acaaaaacca tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat
     9841 gattcctgca tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt
     9901 agtcacagcc ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat
     9961 cttgaaaaac gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt
    10021 ttttggtgca acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat
    10081 gaatccggaa gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg
    10141 tgggatccta ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca
    10201 taaagtagta cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat
    10261 cggtctgatt gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt
    10321 cggtgggatc aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac
    10381 attattcttg ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca
    10441 aatgattgat gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg
    10501 acaagtgggt gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga
    10561 aatgatcaaa ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg
    10621 agtaacatta cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg
    10681 ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc
    10741 tttgatccca ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc
    10801 ttatgctggt ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa
    10861 agaagaatta gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca
    10921 aacttcagct gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt
    10981 agataaagca agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga
    11041 accagaaaac ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac
    11101 gaaacacgct attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat
    11161 caacacagtt gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt
    11221 gacacctgat acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga
    11281 aacatccatg atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa
    11341 aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa
    11401 caatagaaac gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta
    11461 ttgttttctg ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca
    11521 taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt gt
//
LOCUS       DAZKMZ010000289         9991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.086, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000289 DAZKMZ010000000
VERSION     DAZKMZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.086"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1519
                     /locus_tag="ACG8M5_003022"
     CDS             17..1519
                     /locus_tag="ACG8M5_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0360166.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1521..2258
                     /locus_tag="ACG8M5_003023"
     CDS             1521..2258
                     /locus_tag="ACG8M5_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0360167.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(2429..3622)
                     /locus_tag="ACG8M5_003024"
     CDS             complement(2429..3622)
                     /locus_tag="ACG8M5_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0360168.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3701..3907)
                     /locus_tag="ACG8M5_003025"
     CDS             complement(3701..3907)
                     /locus_tag="ACG8M5_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0360169.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(3900..5042)
                     /locus_tag="ACG8M5_003026"
     CDS             complement(3900..5042)
                     /locus_tag="ACG8M5_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0360170.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            5554..6189
                     /locus_tag="ACG8M5_003027"
     CDS             5554..6189
                     /locus_tag="ACG8M5_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0360171.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            6343..6420
                     /locus_tag="ACG8M5_003028"
                     /pseudo
     CDS             6343..6420
                     /locus_tag="ACG8M5_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6734..6781
                     /locus_tag="ACG8M5_003029"
                     /pseudo
     CDS             6734..6781
                     /locus_tag="ACG8M5_003029"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6797..8716
                     /gene="tet(M)"
                     /locus_tag="ACG8M5_003030"
     CDS             6797..8716
                     /gene="tet(M)"
                     /locus_tag="ACG8M5_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0360172.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            8835..9002
                     /locus_tag="ACG8M5_003031"
     CDS             8835..9002
                     /locus_tag="ACG8M5_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0360173.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(9062..9415)
                     /locus_tag="ACG8M5_003032"
     CDS             complement(9062..9415)
                     /locus_tag="ACG8M5_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0360174.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         3079 a         1793 c         1973 g         3146 t
ORIGIN      
        1 atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt tcttaatgac
       61 aggacttgtg acacttgcgt ttggtcatgt tgcacaagct gacaacacta caacggcaga
      121 gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat acgtaattgc
      181 cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt acgaaggcat
      241 cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc gaatggacta
      301 ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg gaatgattgc
      361 cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt cttactttga
      421 tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt atgaagattt
      481 gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt taagcgacca
      541 tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat tgtacgacgg
      601 gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg gttatgcgat
      661 caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa aatatgcatt
      721 tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag gattgaaaga
      781 attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg atggttcaga
      841 aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta tccaaaacaa
      901 ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga tctcctttgc
      961 tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa tacgcgctct
     1021 tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga tggttcttgc
     1081 gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg acttccttgc
     1141 tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg ttcgaggcgg
     1201 gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc tttcttataa
     1261 tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc catcattcgt
     1321 caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg gtgtagtcga
     1381 attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt atgtttactt
     1441 gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag caaaagtttt
     1501 agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta gtcaaaaaat
     1561 acggcgataa cacagtatta aacgacatca atgtatccat caacgaagga gatgtcgtct
     1621 gtatcattgg cccttcaggt tcaggaaaaa gtacattctt gcgttgcttg aatcgtttag
     1681 aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat aaaagcacag
     1741 atatcaactt ggtgagacaa catatcggaa tggtattcca acactttaac ctattccctc
     1801 atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa gaatctagag
     1861 aagaagcaga gaaaaaagca atcaaattac tgcagacagt tggtctagaa gataaaaaag
     1921 atatgtatcc tgaaatgctt tctggtggac aaaaacagcg tgtggctatt gctcgtgctc
     1981 tagcgatgaa ccctgacatc atgctttttg atgagcctac ttctgcactc gatcctgaaa
     2041 tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg acgatggtta
     2101 tcgttacgca cgaaatgggg ttcgctaaag aagtggccaa tcgcgtcatg ttcattgacg
     2161 acggaaactt tttagaagat ggaacgcctc aacaaatctt tgaaaatccg caaaatgaac
     2221 ggacaaaaga tttcttgaat aaagtattaa atatttaaaa taaattatat atgtcttttt
     2281 gactatcttt ttatatcttc ttaaatgtcc gcaaagcctt attttatagg ctctacgggc
     2341 attttgcttt tgtggtaaac ctcacatatc taggtctatc ttcttatatt ttcactatca
     2401 agcgtggtta aaatcgtggt aaatacttct atgcaattag acgctgaacc tctgattttg
     2461 cggtatctat ggacgcatga gcataccagt tcattgtgat actgatattt gaatgcccca
     2521 tgatatactg taaatctttt gggttcatgt tcttgcttgc cagtcttgtg cagaatgtat
     2581 ggcgtagcgt atgcggtgtg atatgtggca atggattgtc cttgtggtgc ttgttgtatt
     2641 tctttaccat acggataaat aaggcgttat aatcaatcgc aactttgggc ttgcccttat
     2701 gattgacaaa taggaagttg ccccgtccgt ctatcacaaa tggttctgcc tttggacgtt
     2761 ttttcataac ctgttgaaat gcctgtatcg tttctttgct taatggtact tgccttattc
     2821 cgctttttgt tttaggcgtt tcaatataat agccctgttc cttgctcttt agtaactggt
     2881 ggtcgataat cacaacttca ttcttgaaat caatgtcggc tactgtcagt ccgcacagtt
     2941 ccgaaatacg aagtcctgtc tttaacagta tcagcacatc atcataatgc ttgtgataca
     3001 cattgtccgt cttgatgaat gagagtaagg cttgttcctg ttcctctgtc aatgcgactt
     3061 tctttttggt atcattttct aggacttcac tcaacttgaa atcaaaggga tttttcctca
     3121 cataatcatc ttgaatggct atgtagaatg acgcttttaa cgagcgttta tggttgttaa
     3181 tggtattgta ggaaaagcct ttgtctttca tgcgtaacgc ccattcctta gcgtcagagg
     3241 gtttgattgt atcaatgctc ctagcaccta acttgtcctc tttcaataac cgcatgagtt
     3301 gttcccgttg tttctgtgtg cttttcttca catttgccct ctgtgcgttc tgtttggcat
     3361 aaagttggca aagcgtcatt ttcttgccag tgctgtcgat accgtcctca atatcccgtc
     3421 ttatctgctg ttccagttct cgaagtgagg gtttttcccg ttttcccttt ggtgtcgggt
     3481 ctgtgggtgt caatctccaa gcatacacat attttgtgtt tccaaatgcg tctacatatt
     3541 tatataagta ttttctgtct gttcgttggc tctctccagt atgcaggata cgacctttgt
     3601 tatcccgtct tttttctttc atggtgtctg ctcctttcct tgttggaaag agccttgata
     3661 tgacttgtgt tcatcataac acatacaagg ctcatttgca ttagatagcg tccaatttat
     3721 caatgacctg ttcaaactgt cggcgtttaa tctgtatgcg gttgccattc ataatgagcc
     3781 agccagcgtc cttgttttcc tctgccaatc gtcttaactt gttttctccg atacggaaat
     3841 actttgacgc ttcttcaatg gtaagggtgt atttttccca tacgggaata tcctgtgcat
     3901 tactcataaa acaccccctc actttcgatt acgtctgcta tgtccgtaca catctgttcc
     3961 acaagcattt cctgcttttc agagaggtgg gtacattcat caatcatttt catgtagttt
     4021 gagcctgtca gcctgtcgat ttcctctatc atcacgattg acggcatgac ctgtctttcc
     4081 agccattgca gagtgcgttc catattgaca ggtacggggt tcatttcaaa aggtaaactg
     4141 tcatgggaaa tgaatatctc ccacagcgga tagtcgggaa attgcacaaa atcagtaata
     4201 aggtaaaaca ccagcccatg agggttatat gtcaataaca attcctcaac tgcctgtact
     4261 gcttttgtac cacgcagacg aatttcaaaa cgattgataa tatccgtatg tttctttttg
     4321 gttgcctgtt ccttttgttt ttcatacagg cagaagtatt ttgtgcttgc ctttgaaccg
     4381 atataaaggg tgcttgctaa attacggttt tttccgctta gttttccacc ctgcacattt
     4441 tcataatttt tacagcgaca atccgcaccg ccatttctgt atttttcaga caaaacggga
     4501 atatccaaca gaccacacat atcattgatt gccaagtcaa aacgtcggat aacaccgtca
     4561 cagtcaagac agcggtttaa aaatgaatac caatctctgt tctgtgcctg caagacatat
     4621 tccatgttgc gtgagcctgc cccttttaat tccaagaata cgcccatgtg ttcgtcatca
     4681 gaagccatga ccttgatttc tccgtaggcg taatgctctt tatagccata atacccatag
     4741 tcataatgga taaaataatc tgctttcatg ccaagcacct ttcggataat ttccaatgcg
     4801 tcagtcgttg gataacggac agacagataa tcaacgagca gataaaaagg cttatcgcag
     4861 atataataat tcagacaacg caggataata ttttgtgttt ccttgctggc gttggctttt
     4921 ccgttctcaa agcggttgat actatatctt gatacatggg taagacttgc aagttctcct
     4981 tgtgaaagac cgcttgcaag acgtttgttc ttcatttcaa gtgcaaattc taaattgttc
     5041 attgtattcc tccaatccca caatagcctt gaaatacctg taaacctcta ttcaaaaagt
     5101 gtgatatgca ctatcttctt aaaacttctc attactactc acaactttgg gattgtatgg
     5161 tttttgactt gtcttttgtg aaatagtgcc cccctgttag ataacggggg cacagacaag
     5221 gctcgcaggc tcgccagccg acaaagcatt tcttcccctt gacacaaggt gtcatggcgg
     5281 taagcctgag aggcttccgc caccgtgtca gaaatacttt gtcagcaagt tctgttttgc
     5341 cttttctaac aagttccatt tcaaccgata ttcatatcta cggatttaat cagataggta
     5401 tttctttttg aatgttttta tatttcattc tattgtgctt attcgattac aagaacatag
     5461 taattcttat atgcttattt gtcaatatct ttttttgcct ttacaaatca tattaagcat
     5521 gatagactta taatagcgaa gatataacga gatatggagg gttatgcaat gaaaataagc
     5581 catttaggaa ataatataca gaccataaga aaatttaggg gaatgaaaca acaggaactt
     5641 gcggacaaaa tcggtatcaa tatgcagagc ctttccaaga ttgaaagagg gttgaactat
     5701 cctgcctatg aaacgctgga aaagataatg gaagtgctgg acgttacgcc caatgaattg
     5761 ttgtcgggag aatggaagta tgtcaatcaa tctgaaaagg aagtctgtca gtttttaaga
     5821 gccgaagaac gtctaaatgc agaactgaaa catggacact atgataactt ctttgacagc
     5881 gaggaagaat ggctggaata tgagttagaa aagttacggg aatatattac agactatatc
     5941 aacgggaaaa gcattgaagc gtccgacctt tacccaatca aagaatttat ccagcatttg
     6001 aagtttcaaa aattattaga ccgctatgat gatttataca gtatggatat gtttggggaa
     6061 agcatagagg gacataagta taagacacct tatcaagtcg tgaaaatgat aaacccgaac
     6121 tcaaaagaag atatggaact gttacgagaa gtattaagga ataacaattt tgatgatgag
     6181 gacgagtaac ttttttcctc ttgcgagaag atacagcaaa caaaaagccc gatcaagagt
     6241 gcaaagcacc accaatggtg gcaaagctct tgacagggct ttttcttttc tgttgtgggc
     6301 aatcaagagg aagaaaggga taaaatctga tttgtggtaa atcaggcaaa ggcaacgcag
     6361 gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa
     6421 caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt
     6481 ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag
     6541 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat
     6601 aattggatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
     6661 tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca
     6721 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
     6781 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
     6841 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
     6901 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
     6961 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
     7021 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
     7081 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
     7141 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
     7201 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
     7261 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
     7321 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
     7381 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
     7441 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
     7501 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
     7561 aattgaatat acaaaaaaaa gacaacgtct tgcatatata cgcctttata gtggagtact
     7621 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     7681 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     7741 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
     7801 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
     7861 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
     7921 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
     7981 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
     8041 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
     8101 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
     8161 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat cgtttcaaaa
     8221 tgcagttatg gaggggatac gctatggctg tgaacaagga ttgtatggtt ggaatgtgac
     8281 ggactgtaaa atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga
     8341 ttttcggatg cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt
     8401 gttagagcca tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata
     8461 caacgatgct cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt
     8521 cattcttagt ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt
     8581 ctttacaaat ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg
     8641 tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt
     8701 caataaaata acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag
     8761 atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata gttattataa
     8821 actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag ataagggaag
     8881 atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa aatttaattg
     8941 aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg cagagccgat
     9001 aaaatgagat taatacaatc tcattttatc ggctctttcc gttatgtatg gattctttta
     9061 attagtcttc gatgtttctt gcttcgttga taccgctggc taaagattcc attaaggata
     9121 gttctttgtc tgtaaagcta tccatgtatt tctctatctg taatcgtcgg gtgcttttta
     9181 ccaagttatt agcaggtaag aaaaattcat caacggaaac atgaagtaac gatacaaggt
     9241 cataaagaac ttgtatgctg gggtgttgcc ctttattttc aatattagtt aagtaccgtg
     9301 ggtcaatttc aatcaatgct cccacttgtt cacgagttaa acctcgtttc aatcgagctt
     9361 ctttaatggc taaaccaaag gctctaaaat catatttatc ttctttttta cgcatagtag
     9421 accacctcta tacattttat tgttcctact gaattaaaaa caggtataga aaaacgtgtt
     9481 atatggttta taggtttata tttaataaaa agcactacta aacgccaata aaaaaaaccg
     9541 ttatatggta gtgctattta cgctgttaaa atattgtata ttacttccaa atggcggttt
     9601 gttggaggtc aacgtcgcca tgaagtacat catatacaat aaatttcctt acattgggtt
     9661 cttgtcaaaa aaagtcgtct atctgcaata gataagtacg tccaccaatg tggttttata
     9721 aatcatatag atagaataac agaagcatgt aaacagagaa ataaatctgt ttatatgctt
     9781 ttttggctat tcagaacttt tttacaaagt ttatttatca gtaatgcaac aaatccccct
     9841 ttcacattgg gactaagagt gaaaggagat aaacgagcaa ggctcacttc ctttcctaga
     9901 cagaaagggg gtgagattat gaggacatct tctttcgagc atatcgtcag attacagttc
     9961 aattctttaa tgctgattgt cattaagaac a
//
LOCUS       DAZKMZ010000290         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.098, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000290 DAZKMZ010000000
VERSION     DAZKMZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.098"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8M5_003033"
     CDS             128..1564
                     /locus_tag="ACG8M5_003033"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0360175.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8M5_003034"
     CDS             1753..4200
                     /locus_tag="ACG8M5_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0360176.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8M5_003035"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8M5_003035"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0360177.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8M5_003036"
     CDS             5487..5693
                     /locus_tag="ACG8M5_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360178.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8M5_003037"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8M5_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0360179.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8M5_003038"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG8M5_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0360180.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKMZ010000291         5238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000291 DAZKMZ010000000
VERSION     DAZKMZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.121"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..770
                     /locus_tag="ACG8M5_003039"
     CDS             78..770
                     /locus_tag="ACG8M5_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0360181.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1097..1594
                     /gene="dfrG"
                     /locus_tag="ACG8M5_003040"
     CDS             1097..1594
                     /gene="dfrG"
                     /locus_tag="ACG8M5_003040"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0360182.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1666..3618)
                     /locus_tag="ACG8M5_003041"
     CDS             complement(1666..3618)
                     /locus_tag="ACG8M5_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0360183.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3625..3861)
                     /locus_tag="ACG8M5_003042"
     CDS             complement(3625..3861)
                     /locus_tag="ACG8M5_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360184.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4263..4553
                     /locus_tag="ACG8M5_003043"
     CDS             4263..4553
                     /locus_tag="ACG8M5_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0360185.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4825..5174)
                     /locus_tag="ACG8M5_003044"
                     /pseudo
     CDS             complement(4825..5174)
                     /locus_tag="ACG8M5_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1763 a          868 c          909 g         1698 t
ORIGIN      
        1 atttaaaaac gcttgacatt gtgtttgaga tcacttactt ttagcatgta aaagtattgg
       61 attaaggagc tatgcaaatg aaaaagaaat ttattactgg tgggattgtc gttgcatttc
      121 tatgtctaat tggctacaca ggcacgcggt acgcaaatga aattattttg tggggtggag
      181 agacagatat tgatacaatc aatgagaatc tgacacagct tgatcataca ttgacagata
      241 aagagcaagc gattacagat cttaccgctc gactagcaca aaaagaacag acattgcaag
      301 attcaaaggc agatgtccaa gaataccaaa caaaagtaaa tgaattagaa aaccaaaaaa
      361 atcaatcggc agcagaaaaa caaaacttag aaagtcaatt agtcagcaag gatgcacaac
      421 tacaaagcaa acaaaatgaa atcaatgtca aactagatga gatcaaccaa aaaaatgatg
      481 aaatcaatca tctgaatgca gattggacag caagactaca agaagcacaa gcaaaacttt
      541 cagaattaca aaatactgta aatggtttga atcaacaaat cgcaacactg acacaagaaa
      601 aaacaaatgt agaaactcag ttaacagata cacaaaagaa attagacgaa gcaactgctg
      661 aaaactcaag cttgaaacaa tatattcaaa agttagaaaa agcaaaagat gaagtggaag
      721 atacagctga taaatctcag caaattgttg aagaacattt gcgtaaataa tctttcttaa
      781 caagtaaacc taaattagta aagaagcaac agtaatctat actaagtagt tcagttttgg
      841 agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta acttacatac
      901 caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga atgaaaattt
      961 gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt caactaacgg
     1021 gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta aattagttaa
     1081 aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga atagagtgat
     1141 tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg ttaaaaatac
     1201 tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg gaagagcctt
     1261 acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta atggttgtga
     1321 aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag aaatttttat
     1381 tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa tgtacatcac
     1441 aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt atgaggaatg
     1501 gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata actactattt
     1561 tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga agaagaacag
     1621 taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt tgaaataaat
     1681 tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag gtgtcaaaac
     1741 ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta gaattgtaat
     1801 cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct tatcaccacg
     1861 agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt gaataaagct
     1921 attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg gttccagagc
     1981 agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg ctaatggatg
     2041 tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa tagcctcttc
     2101 gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag tccgtagaaa
     2161 ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg cttgtccgaa
     2221 ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa aatgatgtgc
     2281 atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa tagagaagtc
     2341 atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta accaatagtg
     2401 agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac tcctattagt
     2461 aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag cggatggttc
     2521 ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat tgagatggtc
     2581 ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc aatcataggc
     2641 aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta tatacgtttg
     2701 catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct tacggacgtt
     2761 atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt tctttgtttg
     2821 aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca gccactccag
     2881 cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac tgactgaaac
     2941 ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt ggtgataagt
     3001 agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct tacattgata
     3061 gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct cattgaatgg
     3121 ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct caacatcttg
     3181 gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat tagataaagg
     3241 ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc agtacatacc
     3301 tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt tcccttttac
     3361 atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga tgcagtaatt
     3421 atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga acaacatttt
     3481 gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag ccctatcttt
     3541 taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct tcgtggctaa
     3601 tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca ttttttcaac
     3661 aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat cgtctaaaat
     3721 gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg
     3781 cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag cgttcttatt
     3841 ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa attgtaacca
     3901 ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt tttatataca
     3961 ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg
     4021 agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac tttttgtact
     4081 ccaaaactga actacttaaa tctatactgc tgcctctttt ttttagtaca tgtccttcat
     4141 cttcgcagga agtgccaata tgattttaag aaaggtaata gacaaaaagt caaaaagata
     4201 aaacagagtt tcttctttac aaagggaatg tatgttcgta taatggtatt gaggtgaagg
     4261 ttatggagaa aaaatatgga acagtagata agatccgtac tttgcaagtt agtgccagac
     4321 cgttgatccg tttcagttta aatggggtca attgtttgat tgcttcccat agtttgaact
     4381 tcctagctga agtagacgaa gggatgaagt tagtcgttac aggttattat aataatagaa
     4441 agcaatttgt tgtgagatcc tatcatgtgt tagggaaacc caaaattgtg gtagaatatg
     4501 agaaaagtct atacccaaga aaaaaggtcc gacaagatga aagaataaac tagaaaacaa
     4561 gcggctgaga caaaaatgtc ctagccgctt gttttggaaa gatggatgca gacgatggga
     4621 tttgaaccca cacgagcata cgctcacaag aacctcaatc tatatataac tacatgtatt
     4681 taagtatttg tgatgaatca ctaaactttc caaactcatt tgagggatat tgcttaaaga
     4741 ttgaacagat caattcgaag ccctattagt attcgctcaa tggggggatc agaggccata
     4801 aaaagagact agtcattact agtctcaggt agtggaattg taggatttta aacgttcctg
     4861 gagagtaccg gtatgtaact cgaagaggtg attatcatag tcgtaaaagt aaattgaaga
     4921 agcctcgcct ttcacacgag aacgtcctgg ttcgacagtt aaacctaaca tttggatttt
     4981 tgagacaaac gaatctatat cttgttcgtc aattttaaaa gcaatatgat tgtatgtctt
     5041 tggcagttta ttcgacgaat tctgcattac tgcaatccat aggtccttaa ctaagaaaaa
     5101 tttttctgga tataatgaaa atttttttgt tgactatcgt agatttcttt tgcatcaaat
     5161 aaggtattta acaattaagt atttaaaaga taacttttaa gttatcttta agtaatgctt
     5221 aaagctatgt tatactct
//
LOCUS       DAZKMZ010000292         4551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000292 DAZKMZ010000000
VERSION     DAZKMZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.134"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..147
                     /locus_tag="ACG8M5_003045"
     CDS             <1..147
                     /locus_tag="ACG8M5_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0360186.1"
                     /translation="GFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKI
                     TMFE"
     gene            501..1979
                     /gene="msr(C)"
                     /locus_tag="ACG8M5_003046"
     CDS             501..1979
                     /gene="msr(C)"
                     /locus_tag="ACG8M5_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0360187.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2026..2472
                     /locus_tag="ACG8M5_003047"
     CDS             2026..2472
                     /locus_tag="ACG8M5_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0360188.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2671..3186)
                     /locus_tag="ACG8M5_003048"
     CDS             complement(2671..3186)
                     /locus_tag="ACG8M5_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0360189.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3303..4505
                     /locus_tag="ACG8M5_003049"
     CDS             3303..4505
                     /locus_tag="ACG8M5_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0360190.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1370 a          756 c          990 g         1435 t
ORIGIN      
        1 ggattcgaag cagatcagtt ggcagaggca tttggtttgg ataaagaccg ttatgttcca
       61 gttattattt tatcaatagg aaaagcggta gaagaaggtt acgagtctgt tcgtttagca
      121 ccagaaaaga tcactatgtt cgaataaaag gttttcagtt actattcagt agctattgct
      181 ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg actgcatcga
      241 tgaaattacg tttcgaactt ttgaataaca actaaataaa ttcaaaagac atgtataaac
      301 aacaatttca tggatggagt atgtccattt tttcgaacga cagtagaata ggtgaaagct
      361 atgacagatt tggtgattta gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc
      421 agtttttttg caaacagatt tttctgctct tttttagtat cgataattgt tgttttacat
      481 gaaataggag tgtaaaacat atggaaaatt tagcagtaaa tataacaaat ctacaagtca
      541 gctttggcaa ccagctagaa ctatctattg attctcttcg tgtctatcag caagatcgta
      601 tagggatcat aggagaaaat ggtgtcggta aatcgacttt gctcaaacta atagccggtg
      661 aactttttcc cgatcatggg aaaatccaaa cagagatcac cttcaactac ctgcctcaat
      721 taacctatct cgctgaagca aaggacctaa atttggaatt agccagtcat ttccagttaa
      781 gactggaaga aacttcagag cggaaatgga gcggagggga agaacgaaag atcgagttga
      841 tacgtcttct ttcttcttat gaacaaggga tgcttctaga cgagccaaca acccatctag
      901 atagaaaaag tattgatcga ctgattgaag agcttcgtta ttattatggt acgctggttt
      961 ttgttagtca tgatcgctat tttctagatg agctagcatc gaaaatctgg gaagtaaaag
     1021 acggagaaat ccgagagttt tcggggaatt atagtgccta tctcactcaa aaggaattgg
     1081 agaaaaagac tcagctacga gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa
     1141 aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga
     1201 aaaagcaaca aatcagactg gatcggttgt cttcctctaa acaaaaagac agtgtacaaa
     1261 aagccatcca aaagaatgcg aaaacattag agagaagact ccaaaaaata ggagaaacaa
     1321 ccaagccgca gcagatgaaa caaatccgtt ttccagtacc aaaatctctt gaactccaca
     1381 gccgctatcc catcatggga caaaatgtcc aattggaaag aagtgggaga acattactgg
     1441 taaatggtga ttttcagttt tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt
     1501 caggtaagac aaccttatta gaacatatcc gcaaacaagg agaaggaatc cttctctctc
     1561 cgaaagtaag ctttcaagta tatcagcaaa agggttatca aatgacatct gaagaatcca
     1621 tcattcgttt tgtcatgaga caaacagagt tttcagaatc gcttgtccgt agtttgctga
     1681 atcacttagg gtttgctcag gaaactttga cgaaaccgtt atgtacatta agtgggggag
     1741 aagcgacccg tctgacgatt gctttgcttt ttactaagcc aagtaatgtg ttgctgttag
     1801 atgaaccgac taattttatt gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa
     1861 tatatccggg aacgattttg tttacttctc atgattctta ctttgtcgag cgtacggctg
     1921 atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga aatttttaaa
     1981 tgtgctacga tgattttcgt gacaaattta aaggagtggt acagattgcc tacaaataaa
     2041 aaagaaggaa taatttttac aacaatgatg tgttttttga tggtcttggg gatgagcgcg
     2101 tataatttat ggctgcatca agatcttcat ttatcaagtt tgttgattgg tctagtgcct
     2161 ggattcgtgg tggctttcat tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt
     2221 gcctttaaac taccagtaaa tccagaaagc aaggtccaaa tgatcttaac tatttcatgc
     2281 ttgatggttt taggaatggt gacatgtatg tctttattcg gtgtgatcat ggaacaagga
     2341 atacctgatc aattattctc tgcttatatg aatgcttgga aaatgaatgt gattgccgct
     2401 ttaccgttgc agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa
     2461 agtaatgaat gatggaacac tgttatttga aaaaaggcct tggcatgctt tttgtcatag
     2521 tctcttgaat gcagttctag aaaaatagtg atccgaacga taggttttgg cctgtcgttc
     2581 ggatcgcttt ttcttttcca actgtattgc tcttttattg aagataattt gctcttcaat
     2641 agatatttct agaaaggttc tgtttatccc ttactcaaaa aactgctgca tttgttctct
     2701 tacttgctga caccattgga gttcctgctg acaaagcata attctgcgct ggatcacaac
     2761 ataacgtcca aattcttgtt tcttgtctag cgagaggtca ttcaattttt tttgattttt
     2821 ttgcatgatt ttgttcagct gttgttctct agtgaatagc agtccagtag ctgttgtttt
     2881 atcgagcgtc gaaaaagcat agatttttgc tagaaattca tctttttgcg taggagcggg
     2941 agtttcctct ttgatccatt cctctacaag agcacgtcct tcatcagtca agtggtagat
     3001 ctttttttga gagtgctcgt ctttgtccaa aacggtcaca taaccctgct ggtctaactt
     3061 ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa aaaattgtga tcagctgctt
     3121 caattcatac ccagaacgtg gtttggataa aagcacgcat aataatatat aggctaaagt
     3181 attcataaaa atcctacctc tcgtcttatt ttctttatta tatcacagta aagtttccac
     3241 gttttacatg tgcatattga catgtaaaat aaatcatgta tgatgctaat agagagaaag
     3301 ggatggaaat attggagaat caaatagata tacaagaaca aacaaatgaa gaaacaaagg
     3361 ggaaaagttc agcgctagtt gtagcagcta cctgtatgat cgcttttatg ggagtgggtc
     3421 tagtagaccc aatcttaaaa acgattgctg cacagttaaa cgctacgcca gctgaaacga
     3481 ccctgttatt tactagttac atgttagtca ctggtgtggt gatgctattt acaggtttct
     3541 tatctagtcg gatcggtttg aaaaaaacat tgatgacagg attgtttatc atcgttgtat
     3601 tcgctggtct tggcggatta tctgggaata tcggaatgct gattgggtta cgtgccggct
     3661 ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc aacgatcgtc agtattttag
     3721 ttggtaacac agaaaaagca atcatgatgt atgaagcagc acttggctgc ggaatggctg
     3781 ttggccctct agtcggtggg gtcttgggca gtggttcatg gcgttatcca ttttttgggg
     3841 tagcattttt gatgttttgc ggttttatag ccctagcggt acttttacct gaaactgaga
     3901 aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat cataaacttc
     3961 gttcagttgg actaatcgca ttgttatata atttcggatt ctttacttta cttgcgtatg
     4021 cgccattttt attgattggc tattctgaat tagaagtcgg ttttgtattt tttggctggg
     4081 gtgtgcttct ggctattgct tcgattttcg ttgcaccggc tcttgagcgg aagtttacga
     4141 cttatcggac aattttagca gcatttgtct tattcattat ttgtttgctg ttattaggag
     4201 ctggaacttc tatgccagtc cttgtaccga tcagcattgt gttagcagga tttttccaag
     4261 gaattatcaa tacactgttg acgacgattg ctatggaaat accaggtctg caacgaaacg
     4321 ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg agctttagca ccatttattg
     4381 caggtgtgat tggtgaacat attggtgaaa gctatgcgtt ttattttgca ggtgccattg
     4441 ttcttatcag tgtatgttgt atcatttatc accgtaatta ttttgctaca aatgaagaag
     4501 aataagaaat cagaggttgt gaaaaagctg tcctgcatca agtcataaga a
//
LOCUS       DAZKMZ010000293         2789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.173, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000293 DAZKMZ010000000
VERSION     DAZKMZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.173"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1087
                     /locus_tag="ACG8M5_003050"
     CDS             119..1087
                     /locus_tag="ACG8M5_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0360191.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1080..2111
                     /gene="vanA"
                     /locus_tag="ACG8M5_003051"
     CDS             1080..2111
                     /gene="vanA"
                     /locus_tag="ACG8M5_003051"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0360192.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2117..2725
                     /locus_tag="ACG8M5_003052"
     CDS             2117..2725
                     /locus_tag="ACG8M5_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0360193.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          824 a          539 c          683 g          743 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccctt cagagaaaga tttgacaaga
       61 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
      121 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
      181 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
      241 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
      301 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
      361 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
      421 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
      481 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
      541 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
      601 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
      661 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
      721 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
      781 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
      841 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
      901 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
      961 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     1021 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     1081 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     1141 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     1201 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     1261 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     1321 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     1381 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     1441 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     1501 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     1561 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     1621 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     1681 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     1741 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     1801 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     1861 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     1921 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     1981 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     2041 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     2101 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     2161 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     2221 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     2281 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     2341 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     2401 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     2461 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     2521 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     2581 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     2641 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     2701 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac ggttctgttg
     2761 caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZKMZ010000294         2279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.192, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000294 DAZKMZ010000000
VERSION     DAZKMZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.192"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..788
                     /locus_tag="ACG8M5_003053"
     CDS             54..788
                     /locus_tag="ACG8M5_003053"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0360194.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            821..1729
                     /locus_tag="ACG8M5_003054"
     CDS             821..1729
                     /locus_tag="ACG8M5_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0360195.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1726..2206
                     /locus_tag="ACG8M5_003055"
                     /pseudo
     CDS             1726..2206
                     /locus_tag="ACG8M5_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          796 a          322 c          514 g          647 t
ORIGIN      
        1 agtgaatgaa attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag
       61 aaaacaaata tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga
      121 aaggactggc tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg
      181 gtaagcgtgt gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa
      241 acggtgcttc ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag
      301 gaaaaaccca ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc
      361 cagaggagag ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg
      421 agaatttaat aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag
      481 ttgaacatcc tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag
      541 aaatactgca tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt
      601 tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt
      661 caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac atgatggata
      721 ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga
      781 agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat
      841 gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg tgacccttga
      901 ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg atattacata
      961 ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa
     1021 tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt
     1081 ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga
     1141 agagttagat aattacctaa agggcgataa attaataaag gttctaattg ataaagattg
     1201 tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc
     1261 aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg
     1321 attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga
     1381 gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg
     1441 caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc
     1501 cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt
     1561 gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga
     1621 taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg
     1681 cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg
     1741 aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag
     1801 attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca
     1861 tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa
     1921 tatattgaca atactgataa gataatatat cttttatata gaagatatcg ccgtatgtaa
     1981 ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag aatgggcaaa
     2041 gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta tagcttgtaa
     2101 attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     2161 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     2221 tgaattggag ttcgtcttgt ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZKMZ010000295         2199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.196, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000295 DAZKMZ010000000
VERSION     DAZKMZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.196"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..1120
                     /locus_tag="ACG8M5_003056"
     CDS             209..1120
                     /locus_tag="ACG8M5_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0360196.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1273..1758
                     /gene="vanZ-A"
                     /locus_tag="ACG8M5_003057"
     CDS             1273..1758
                     /gene="vanZ-A"
                     /locus_tag="ACG8M5_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0360197.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          773 a          318 c          419 g          689 t
ORIGIN      
        1 aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg
       61 gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc
      121 acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc
      181 aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt
      241 attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt
      301 cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga
      361 aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat
      421 caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa
      481 acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga
      541 aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt
      601 tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg
      661 ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt
      721 aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc
      781 ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca
      841 atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa
      901 gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag
      961 tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca
     1021 tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac
     1081 agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga atgaaaccaa
     1141 cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag ctttttaatt
     1201 taaaacccac gttaactagg acattgctat actaatgata caacttaaac aaaagaatta
     1261 gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata tttagtgaca
     1321 ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt taattatcat
     1381 caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga gatgatagat
     1441 aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa agaaatcgga
     1501 tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat aattcaattt
     1561 atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt tggaggcttt
     1621 cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa attagacaga
     1681 gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg tacccattta
     1741 agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata cacataaaga
     1801 tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga
     1861 agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt aacaaagaaa
     1921 gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc cttaacgttg
     1981 taaatccgca ttttcctgac ggtaccccca ctagcagcca tgcttttttt gaatctttgt
     2041 aggacgtttt aaagagaatt acccacttta aaattaaaca tgcgcttaga atcgctttag
     2101 gaaacacgat ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta
     2161 cctacttttg gaataatcac aaatcacaag tgattaatc
//
LOCUS       DAZKMZ010000296         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3583
            SAMEA115706804-rid22575853.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZKMZ010000296 DAZKMZ010000000
VERSION     DAZKMZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706804
            Sequence Read Archive: ERR13196538
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287784.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287784.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:54:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,059
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 39 of 91
            Pseudo Genes (incomplete)         :: 47 of 91
            Pseudo Genes (internal stop)      :: 22 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706804-rid22575853.guided.239"
                     /isolate="VRE3583"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8M5_003058"
     CDS             26..76
                     /locus_tag="ACG8M5_003058"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0360198.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8M5_003059"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8M5_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0360199.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
